Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28025.t1 | KEH20775.1 | 93.5 | 107 | 7 | 0 | 1 | 107 | 1 | 107 | 1.10E-49 | 205.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28025.t1 | A0A072U4M2 | 93.5 | 107 | 7 | 0 | 1 | 107 | 1 | 107 | 7.8e-50 | 205.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050060 | MS.gene28025 | 0.803759 | 2.99E-49 | -1.69E-46 |
| MS.gene052469 | MS.gene28025 | 0.805089 | 1.58E-49 | -1.69E-46 |
| MS.gene057396 | MS.gene28025 | 0.801684 | 8.02E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28025.t1 | MTR_8g089830 | 93.458 | 107 | 7 | 0 | 1 | 107 | 1 | 107 | 1.05e-72 | 211 |
| MS.gene28025.t1 | MTR_8g089825 | 75.238 | 105 | 21 | 4 | 1 | 103 | 1 | 102 | 2.15e-49 | 152 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTGTTGTGGTTTCGGTTT+TGG | 0.230297 | 4.4:-11288670 | None:intergenic |
| ACCCGTAATTGTTGTGGTTT+CGG | 0.263916 | 4.4:-11288676 | None:intergenic |
| AACCGAAACCACAACAATTA+CGG | 0.350207 | 4.4:+11288674 | MS.gene28025:CDS |
| GATTGATCTTCCTCAACTAT+TGG | 0.360870 | 4.4:-11288782 | None:intergenic |
| CCTCATTGATGACATGAGTT+TGG | 0.382010 | 4.4:-11288954 | None:intergenic |
| ACCGAAACCACAACAATTAC+GGG | 0.390353 | 4.4:+11288675 | MS.gene28025:CDS |
| AGATCAATCCTACTATTTAG+AGG | 0.394992 | 4.4:+11288796 | MS.gene28025:CDS |
| TTACGAACCCGCATACTATC+CGG | 0.409986 | 4.4:+11288718 | MS.gene28025:CDS |
| TACGAACCCGCATACTATCC+GGG | 0.436849 | 4.4:+11288719 | MS.gene28025:CDS |
| ACGACGAACCTCTAAATAGT+AGG | 0.482372 | 4.4:-11288804 | None:intergenic |
| AGACCGATCGACGAGGTTTG+AGG | 0.483942 | 4.4:+11288840 | MS.gene28025:CDS |
| GATCGACGAGGTTTGAGGTA+TGG | 0.517220 | 4.4:+11288845 | MS.gene28025:CDS |
| GCGTCGTGTTGCAATATATG+AGG | 0.518107 | 4.4:+11288757 | MS.gene28025:CDS |
| ATACCTCAAACCTCGTCGAT+CGG | 0.542925 | 4.4:-11288843 | None:intergenic |
| TTATTCAACACGAGCATAGG+AGG | 0.567055 | 4.4:+11288921 | MS.gene28025:CDS |
| CAAACTCATGTCATCAATGA+GGG | 0.571315 | 4.4:+11288955 | MS.gene28025:CDS |
| GACTCCACCCGTAATTGTTG+TGG | 0.580902 | 4.4:-11288682 | None:intergenic |
| GTGATGCCCCGGATAGTATG+CGG | 0.591042 | 4.4:-11288726 | None:intergenic |
| CCAAACTCATGTCATCAATG+AGG | 0.606593 | 4.4:+11288954 | MS.gene28025:CDS |
| TGATGCCCCGGATAGTATGC+GGG | 0.606955 | 4.4:-11288725 | None:intergenic |
| ATATGAGGAACCAATAGTTG+AGG | 0.635820 | 4.4:+11288772 | MS.gene28025:CDS |
| TCAACACGAGCATAGGAGGA+CGG | 0.662040 | 4.4:+11288925 | MS.gene28025:CDS |
| GAAACCACAACAATTACGGG+TGG | 0.673197 | 4.4:+11288678 | MS.gene28025:CDS |
| ACGAACCCGCATACTATCCG+GGG | 0.673282 | 4.4:+11288720 | MS.gene28025:CDS |
| GAGACTGAGACCGATCGACG+AGG | 0.700755 | 4.4:+11288833 | MS.gene28025:CDS |
| AAGTGTTGATGAAGAAGCTG+AGG | 0.703686 | 4.4:+11288895 | MS.gene28025:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGATCAATCCTACTATTTAG+AGG | + | chr4.4:11288796-11288815 | MS.gene28025:CDS | 30.0% | |
| AACCGAAACCACAACAATTA+CGG | + | chr4.4:11288674-11288693 | MS.gene28025:CDS | 35.0% | |
| ATATGAGGAACCAATAGTTG+AGG | + | chr4.4:11288772-11288791 | MS.gene28025:CDS | 35.0% | |
| CAAACTCATGTCATCAATGA+GGG | + | chr4.4:11288955-11288974 | MS.gene28025:CDS | 35.0% | |
| GATTGATCTTCCTCAACTAT+TGG | - | chr4.4:11288785-11288804 | None:intergenic | 35.0% | |
| ! | AATTGTTGTGGTTTCGGTTT+TGG | - | chr4.4:11288673-11288692 | None:intergenic | 35.0% |
| ! | CTTTTATTCAACACGAGCAT+AGG | + | chr4.4:11288918-11288937 | MS.gene28025:CDS | 35.0% |
| ACCCGTAATTGTTGTGGTTT+CGG | - | chr4.4:11288679-11288698 | None:intergenic | 40.0% | |
| ACCGAAACCACAACAATTAC+GGG | + | chr4.4:11288675-11288694 | MS.gene28025:CDS | 40.0% | |
| ACGACGAACCTCTAAATAGT+AGG | - | chr4.4:11288807-11288826 | None:intergenic | 40.0% | |
| CCAAACTCATGTCATCAATG+AGG | + | chr4.4:11288954-11288973 | MS.gene28025:CDS | 40.0% | |
| TTATTCAACACGAGCATAGG+AGG | + | chr4.4:11288921-11288940 | MS.gene28025:CDS | 40.0% | |
| ! | CCTCATTGATGACATGAGTT+TGG | - | chr4.4:11288957-11288976 | None:intergenic | 40.0% |
| !! | AAGTGTTGATGAAGAAGCTG+AGG | + | chr4.4:11288895-11288914 | MS.gene28025:CDS | 40.0% |
| ATACCTCAAACCTCGTCGAT+CGG | - | chr4.4:11288846-11288865 | None:intergenic | 45.0% | |
| GAAACCACAACAATTACGGG+TGG | + | chr4.4:11288678-11288697 | MS.gene28025:CDS | 45.0% | |
| GCGTCGTGTTGCAATATATG+AGG | + | chr4.4:11288757-11288776 | MS.gene28025:CDS | 45.0% | |
| TTACGAACCCGCATACTATC+CGG | + | chr4.4:11288718-11288737 | MS.gene28025:CDS | 45.0% | |
| ! | TTGCAACACGACGCTTTTGA+TGG | - | chr4.4:11288751-11288770 | None:intergenic | 45.0% |
| GACTCCACCCGTAATTGTTG+TGG | - | chr4.4:11288685-11288704 | None:intergenic | 50.0% | |
| TACGAACCCGCATACTATCC+GGG | + | chr4.4:11288719-11288738 | MS.gene28025:CDS | 50.0% | |
| TCAACACGAGCATAGGAGGA+CGG | + | chr4.4:11288925-11288944 | MS.gene28025:CDS | 50.0% | |
| ! | GATCGACGAGGTTTGAGGTA+TGG | + | chr4.4:11288845-11288864 | MS.gene28025:CDS | 50.0% |
| ACGAACCCGCATACTATCCG+GGG | + | chr4.4:11288720-11288739 | MS.gene28025:CDS | 55.0% | |
| AGACCGATCGACGAGGTTTG+AGG | + | chr4.4:11288840-11288859 | MS.gene28025:CDS | 55.0% | |
| GTGATGCCCCGGATAGTATG+CGG | - | chr4.4:11288729-11288748 | None:intergenic | 55.0% | |
| TGATGCCCCGGATAGTATGC+GGG | - | chr4.4:11288728-11288747 | None:intergenic | 55.0% | |
| !!! | CGCTTTTGATGGTGATGCCC+CGG | - | chr4.4:11288740-11288759 | None:intergenic | 55.0% |
| GAGACTGAGACCGATCGACG+AGG | + | chr4.4:11288833-11288852 | MS.gene28025:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 11288662 | 11288985 | 11288662 | ID=MS.gene28025 |
| chr4.4 | mRNA | 11288662 | 11288985 | 11288662 | ID=MS.gene28025.t1;Parent=MS.gene28025 |
| chr4.4 | exon | 11288662 | 11288985 | 11288662 | ID=MS.gene28025.t1.exon1;Parent=MS.gene28025.t1 |
| chr4.4 | CDS | 11288662 | 11288985 | 11288662 | ID=cds.MS.gene28025.t1;Parent=MS.gene28025.t1 |
| Gene Sequence |
| Protein sequence |
