AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28675

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28675.t1 MTR_4g091530 95.853 434 15 3 1 434 1 431 0.0 835
MS.gene28675.t1 MTR_1g109470 47.959 196 89 6 6 191 24 216 3.81e-45 167
MS.gene28675.t1 MTR_6g082400 53.247 154 61 7 28 175 58 206 5.66e-39 149
MS.gene28675.t1 MTR_8g106245 47.340 188 75 7 16 190 30 206 7.01e-36 139
MS.gene28675.t1 MTR_2g010160 53.448 116 53 1 16 131 31 145 4.41e-35 135
MS.gene28675.t1 MTR_4g116520 45.390 141 70 3 28 166 151 286 1.69e-31 128
MS.gene28675.t1 MTR_4g092850 53.774 106 46 2 28 131 38 142 2.21e-31 125
MS.gene28675.t1 MTR_2g461180 45.255 137 68 3 28 162 214 345 2.16e-30 125
MS.gene28675.t1 MTR_6g033130 41.772 158 81 4 28 181 190 340 6.71e-28 117
MS.gene28675.t1 MTR_6g033130 41.772 158 81 4 28 181 190 340 6.97e-28 117
MS.gene28675.t1 MTR_5g067160 39.344 183 99 7 27 199 41 221 1.07e-27 114
MS.gene28675.t1 MTR_1g090020 37.186 199 113 5 2 192 160 354 3.35e-27 115
MS.gene28675.t1 MTR_8g442400 43.284 134 71 3 29 162 179 307 4.20e-27 115
MS.gene28675.t1 MTR_2g005700 40.237 169 84 5 28 183 12 176 1.20e-26 109
MS.gene28675.t1 MTR_5g067120 47.287 129 56 4 28 147 26 151 2.08e-26 110
MS.gene28675.t1 MTR_3g077030 54.545 88 39 1 29 116 9 95 6.41e-26 104
MS.gene28675.t1 MTR_4g128650 42.857 168 85 6 23 187 22 181 1.27e-24 107
MS.gene28675.t1 MTR_4g105020 52.222 90 42 1 29 118 9 97 1.31e-24 100
MS.gene28675.t1 MTR_5g032960 47.115 104 53 1 29 132 16 117 4.75e-24 100
MS.gene28675.t1 MTR_5g488180 43.443 122 63 3 28 145 3 122 2.15e-21 92.4
MS.gene28675.t1 MTR_5g088090 43.443 122 63 3 28 145 3 122 2.15e-21 92.4
MS.gene28675.t1 MTR_5g088140 43.443 122 63 3 28 145 3 122 2.15e-21 92.4
MS.gene28675.t1 MTR_5g088620 42.623 122 64 3 28 145 3 122 2.94e-21 92.0
MS.gene28675.t1 MTR_5g088120 42.623 122 64 3 28 145 3 122 4.09e-21 91.3
MS.gene28675.t1 MTR_5g488190 42.623 122 64 3 28 145 3 122 4.09e-21 91.3
MS.gene28675.t1 MTR_5g088590 42.623 122 64 3 28 145 3 122 4.68e-21 92.0
MS.gene28675.t1 MTR_5g088040 42.623 122 64 4 28 145 3 122 1.49e-20 90.1
MS.gene28675.t1 MTR_5g488200 46.392 97 48 3 50 145 183 276 2.13e-16 80.9
MS.gene28675.t1 MTR_5g067200 50.000 82 39 2 51 132 14 93 2.54e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28675.t1 AT4G05150 45.356 463 171 18 6 422 38 464 1.84e-92 287
MS.gene28675.t1 AT2G01190 50.649 154 70 5 28 178 74 224 3.87e-40 152
MS.gene28675.t1 AT5G64430 43.284 201 78 9 11 187 11 199 1.18e-33 132
MS.gene28675.t1 AT5G09620 45.685 197 72 10 11 187 11 192 3.80e-33 131
MS.gene28675.t1 AT5G09620 45.685 197 72 10 11 187 11 192 4.02e-33 130
MS.gene28675.t1 AT5G49920 56.481 108 40 3 25 132 6 106 3.80e-32 124
MS.gene28675.t1 AT3G18230 47.024 168 78 7 28 192 63 222 3.22e-31 126
MS.gene28675.t1 AT5G57610 45.946 148 72 4 23 168 18 159 1.49e-30 125
MS.gene28675.t1 AT3G46920 38.365 159 96 2 18 175 64 221 2.39e-29 121
MS.gene28675.t1 AT3G46920 39.597 149 88 2 28 175 8 155 5.78e-29 120
MS.gene28675.t1 AT3G26510 48.148 108 49 2 29 132 10 114 4.26e-28 110
MS.gene28675.t1 AT3G26510 48.148 108 49 2 29 132 10 114 4.26e-28 110
MS.gene28675.t1 AT3G26510 48.148 108 49 2 29 132 10 114 4.26e-28 110
MS.gene28675.t1 AT3G26510 48.148 108 49 2 29 132 10 114 4.26e-28 110
MS.gene28675.t1 AT3G26510 48.148 108 49 2 29 132 10 114 4.26e-28 110
MS.gene28675.t1 AT3G26510 48.148 108 49 2 29 132 10 114 4.26e-28 110
MS.gene28675.t1 AT2G35050 42.222 135 72 3 28 162 176 304 8.67e-27 114
MS.gene28675.t1 AT2G35050 42.222 135 72 3 28 162 176 304 8.67e-27 114
MS.gene28675.t1 AT1G70640 45.192 104 53 1 29 132 8 107 8.90e-27 106
MS.gene28675.t1 AT3G48240 49.038 104 46 2 29 132 14 110 1.20e-26 105
MS.gene28675.t1 AT1G79570 48.571 105 49 3 28 131 176 276 1.73e-26 113
MS.gene28675.t1 AT1G79570 48.571 105 49 3 28 131 176 276 2.46e-26 112
MS.gene28675.t1 AT1G79570 48.571 105 49 3 28 131 176 276 2.46e-26 112
MS.gene28675.t1 AT1G79570 48.571 105 49 3 28 131 176 276 2.76e-26 112
MS.gene28675.t1 AT1G79570 48.571 105 49 3 28 131 176 276 2.76e-26 112
MS.gene28675.t1 AT1G16270 49.524 105 48 3 28 131 164 264 8.16e-26 111
MS.gene28675.t1 AT1G16270 49.524 105 48 3 28 131 164 264 8.16e-26 111
MS.gene28675.t1 AT1G16270 49.524 105 48 3 28 131 164 264 8.16e-26 111
MS.gene28675.t1 AT1G04700 42.282 149 74 5 1 142 89 232 2.19e-25 109
MS.gene28675.t1 AT1G04700 42.282 149 74 5 1 142 89 232 2.19e-25 109
MS.gene28675.t1 AT1G04700 42.282 149 74 5 1 142 89 232 2.62e-25 109
MS.gene28675.t1 AT3G24715 45.045 111 58 2 28 138 175 282 1.48e-24 107
MS.gene28675.t1 AT3G24715 45.045 111 58 2 28 138 175 282 1.48e-24 107
MS.gene28675.t1 AT3G24715 45.045 111 58 2 28 138 175 282 1.48e-24 107
MS.gene28675.t1 AT1G25300 46.729 107 50 2 27 132 9 109 8.68e-24 100
MS.gene28675.t1 AT5G63130 47.115 104 48 2 29 132 15 111 9.76e-23 95.5
MS.gene28675.t1 AT5G63130 47.115 104 48 2 29 132 15 111 9.76e-23 95.5
MS.gene28675.t1 AT5G16220 42.276 123 65 3 13 132 18 137 9.31e-21 94.7

Find 151 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTTAAACGGTGGCGTTTC+GGG 0.121829 4.2:-20767292 MS.gene28675:CDS
CTTCGTGAATCTTCTCCTTC+TGG 0.147069 4.2:+20767373 None:intergenic
TTTGCAACCACAATTGAAAA+TGG 0.213362 4.2:-20766951 MS.gene28675:CDS
TGCTTTAAACGGTGGCGTTT+CGG 0.232989 4.2:-20767293 MS.gene28675:CDS
GAAGTACCTGATTATCTATT+CGG 0.242759 4.2:-20767229 MS.gene28675:CDS
CACAAACTCTCTAAACTTTC+CGG 0.264017 4.2:-20767636 MS.gene28675:intron
CAGGCGATGGCGGGGACTGG+TGG 0.270232 4.2:-20766539 MS.gene28675:CDS
AGATGTCGTTGTTGTTCTTT+AGG 0.270705 4.2:+20767172 None:intergenic
GTAGGACTGTAGGGCTGAAC+CGG 0.304171 4.2:+20766644 None:intergenic
GGTTCCGGTTTATTATATTC+AGG 0.315057 4.2:-20766834 MS.gene28675:CDS
CGGGTATCATCAAATGATTC+CGG 0.316503 4.2:-20766708 MS.gene28675:CDS
GCAGAGAGGTGGAACGGGTC+GGG 0.349671 4.2:-20766507 MS.gene28675:CDS
CCACAAGAAGGTGCTTATTC+TGG 0.354773 4.2:-20766872 MS.gene28675:CDS
TTGTTCTTTAGGTCGTGATT+CGG 0.357506 4.2:+20767183 None:intergenic
GTCCGCTTCATGTGCAGTTT+CGG 0.358673 4.2:-20767762 MS.gene28675:CDS
AAGATCAGAAGCTTCTTCTA+TGG 0.363823 4.2:-20767257 MS.gene28675:CDS
TTCGTGAATCTTCTCCTTCT+GGG 0.364740 4.2:+20767374 None:intergenic
AAACAACTCCGGTCCAGTTC+CGG 0.378337 4.2:-20766570 MS.gene28675:CDS
GCCTGGTGGTAAACCGGAAC+TGG 0.384934 4.2:+20766557 None:intergenic
TCATCATGCACAACCGGTTC+CGG 0.393242 4.2:-20766849 MS.gene28675:CDS
CGGAAAGTTTAGAGAGTTTG+TGG 0.396173 4.2:+20767637 None:intergenic
GGCGATGGCGGGGACTGGTG+GGG 0.397419 4.2:-20766537 MS.gene28675:CDS
AGGCGATGGCGGGGACTGGT+GGG 0.397436 4.2:-20766538 MS.gene28675:CDS
GGTTTATTATATTCAGGGTT+CGG 0.398190 4.2:-20766828 MS.gene28675:CDS
CACCAGTCCCCGCCATCGCC+TGG 0.401694 4.2:+20766540 None:intergenic
TAAAAGAGAAAACTGGTTCA+TGG 0.401787 4.2:-20767317 MS.gene28675:CDS
AGTGAAGTGCAGGGTTAATC+TGG 0.404619 4.2:+20766921 None:intergenic
TGTTGTTGAGATTGTGGTTG+TGG 0.406405 4.2:+20766896 None:intergenic
ACAAGATAATGTTTCCAATT+CGG 0.408274 4.2:-20767137 MS.gene28675:CDS
AAGTACCTGATTATCTATTC+GGG 0.410034 4.2:-20767228 MS.gene28675:CDS
TAATGTTTCCAATTCGGATC+CGG 0.413615 4.2:-20767131 MS.gene28675:CDS
TGTTCTTTAGGTCGTGATTC+GGG 0.416472 4.2:+20767184 None:intergenic
GTTCCGGTTTATTATATTCA+GGG 0.424050 4.2:-20766833 MS.gene28675:CDS
TGATAACCAAGTTTCTGAAC+AGG 0.424232 4.2:-20767008 MS.gene28675:CDS
AGGAGAACCCGGATCCGAAT+TGG 0.424926 4.2:+20767123 None:intergenic
TCCAACCCGAATAGATAATC+AGG 0.432366 4.2:+20767223 None:intergenic
CAGAGAGGTGGAACGGGTCG+GGG 0.432431 4.2:-20766506 MS.gene28675:CDS
TTCTGGTCATCATGCACAAC+CGG 0.433210 4.2:-20766855 MS.gene28675:CDS
GGGGATGAGGCATGTGGCCC+CGG 0.434471 4.2:-20766663 MS.gene28675:CDS
GGCACCTGAGGTTGCATCAC+TGG 0.443860 4.2:+20766734 None:intergenic
GCCATCGCCTGGTGGTAAAC+CGG 0.444107 4.2:+20766551 None:intergenic
TCATCAAATGATTCCGGGTA+CGG 0.445583 4.2:-20766702 MS.gene28675:CDS
AGTATCAGTTCACTCTTAAA+CGG 0.446300 4.2:-20767346 MS.gene28675:CDS
GTTCATCTAAAAGAGAAAAC+TGG 0.446694 4.2:-20767324 MS.gene28675:CDS
GACGTAACGGAGCTGATTAT+CGG 0.447625 4.2:+20767716 None:intergenic
CACCAGGCGATGGCGGGGAC+TGG 0.448982 4.2:-20766542 MS.gene28675:CDS
GGTTGTGGGTAGTGAAGTGC+AGG 0.449200 4.2:+20766911 None:intergenic
AGAAACTTGGTTATCAAGCT+TGG 0.450096 4.2:+20767015 None:intergenic
GGTTGCATCACTGGATGATA+TGG 0.450911 4.2:+20766743 None:intergenic
AAGTGTATGGTGCGGGGATG+AGG 0.451803 4.2:-20766676 MS.gene28675:CDS
TAGGACTGTAGGGCTGAACC+GGG 0.455812 4.2:+20766645 None:intergenic
GTTGTTGAGATTGTGGTTGT+GGG 0.456391 4.2:+20766897 None:intergenic
AACGCGTCTAAGTCTTCGTT+TGG 0.459798 4.2:+20767502 None:intergenic
AATGTTTCCAATTCGGATCC+GGG 0.461143 4.2:-20767130 MS.gene28675:CDS
TGAATATAATAAACCGGAAC+CGG 0.462037 4.2:+20766836 None:intergenic
GTTTACCACCAGGCGATGGC+GGG 0.463152 4.2:-20766548 MS.gene28675:CDS
GAACCCTGAATATAATAAAC+CGG 0.464629 4.2:+20766830 None:intergenic
GGGTATCATCAAATGATTCC+GGG 0.465587 4.2:-20766707 MS.gene28675:CDS
AGGAGCGTCGTTGAAGTGAT+TGG 0.466489 4.2:+20767791 None:intergenic
GGTGGAAGATTCGGAATAGA+AGG 0.470280 4.2:+20767046 None:intergenic
ACGGGTCAAGTGTATGGTGC+GGG 0.473008 4.2:-20766683 MS.gene28675:CDS
TGTGGTTGTTGTTGAGATTG+TGG 0.475188 4.2:+20766890 None:intergenic
ATGCAACCTCAGGTGCCAAT+CGG 0.477046 4.2:-20766728 MS.gene28675:CDS
GAGCTGCAGAGAGGTGGAAC+GGG 0.478542 4.2:-20766512 MS.gene28675:CDS
GAAGAAGAAACAACCGGAGC+AGG 0.478610 4.2:+20767103 None:intergenic
CCGGGTACGGGTCAAGTGTA+TGG 0.478623 4.2:-20766689 MS.gene28675:CDS
TGGTTCATGGATGCTTTAAA+CGG 0.480187 4.2:-20767304 MS.gene28675:CDS
ATTCAGGGTTCGGTTCAACC+TGG 0.482983 4.2:-20766818 MS.gene28675:CDS
TTTGTGGACAAGAGCGGAGA+AGG 0.483623 4.2:+20767653 None:intergenic
GAAGGAGATGGAGCGGTTGA+CGG 0.488287 4.2:+20767671 None:intergenic
AGCGGTTGACGGCGACGATA+CGG 0.490801 4.2:+20767682 None:intergenic
AACGGTGAAGAAGAAACAAC+CGG 0.494392 4.2:+20767097 None:intergenic
AGCTGATTATCGGAGGGGCG+AGG 0.494867 4.2:+20767726 None:intergenic
TCCAGTTCCGGTTTACCACC+AGG 0.495490 4.2:-20766558 MS.gene28675:CDS
CCATGTCCTTGCATATGAAC+CGG 0.495902 4.2:+20766788 None:intergenic
TTCTATTCCGAATCTTCCAC+CGG 0.501262 4.2:-20767044 MS.gene28675:CDS
ATATGAACCGGTACATTCCC+AGG 0.501983 4.2:+20766800 None:intergenic
GCTGATTATCGGAGGGGCGA+GGG 0.505226 4.2:+20767727 None:intergenic
CGCCGAAACTGCACATGAAG+CGG 0.507791 4.2:+20767760 None:intergenic
TGCAACCTCAGGTGCCAATC+GGG 0.508511 4.2:-20766727 MS.gene28675:CDS
AATCTTCTCCTTCTGGGAAG+AGG 0.515496 4.2:+20767380 None:intergenic
CGCTTCATGTGCAGTTTCGG+CGG 0.517726 4.2:-20767759 MS.gene28675:CDS
ACCTGATTATCTATTCGGGT+TGG 0.519603 4.2:-20767224 MS.gene28675:CDS
CATCAAATGATTCCGGGTAC+GGG 0.524938 4.2:-20766701 MS.gene28675:CDS
GCGGTTGACGGCGACGATAC+GGG 0.525392 4.2:+20767683 None:intergenic
CGTCTCTTCCTCTTCCCAGA+AGG 0.527288 4.2:-20767388 MS.gene28675:CDS
AAGTGATTGGAACGAGGGGA+AGG 0.530356 4.2:+20767804 None:intergenic
AAATCAAACTGAAACTGAAA+CGG 0.530818 4.2:-20766975 MS.gene28675:CDS
TGCAGAGAGGTGGAACGGGT+CGG 0.531048 4.2:-20766508 MS.gene28675:CDS
GACAAGAGCGGAGAAGGAGA+TGG 0.532990 4.2:+20767659 None:intergenic
GTGGTAAACCGGAACTGGAC+CGG 0.543478 4.2:+20766562 None:intergenic
ACAACCGGAGCAGGAGAACC+CGG 0.546578 4.2:+20767112 None:intergenic
TCCGGTTTACCACCAGGCGA+TGG 0.551585 4.2:-20766552 MS.gene28675:CDS
TGATATGGTTGTTGAACATA+GGG 0.551634 4.2:+20766758 None:intergenic
ATCATTTGATGATACCCGAT+TGG 0.554784 4.2:+20766713 None:intergenic
GGATCCGGGTTCTCCTGCTC+CGG 0.555159 4.2:-20767116 MS.gene28675:CDS
TCATCCAGTGATGCAACCTC+AGG 0.556282 4.2:-20766738 MS.gene28675:CDS
GGGTGTCGCCGCCGACGTAA+CGG 0.556591 4.2:+20767703 None:intergenic
GGTTTACCACCAGGCGATGG+CGG 0.559881 4.2:-20766549 MS.gene28675:CDS
TACGGGTCAAGTGTATGGTG+CGG 0.564439 4.2:-20766684 MS.gene28675:CDS
TTCAGGGTTCGGTTCAACCT+GGG 0.567318 4.2:-20766817 MS.gene28675:CDS
ACCACCGTGGTAGGACTGTA+GGG 0.569080 4.2:+20766635 None:intergenic
CCATACACTTGACCCGTACC+CGG 0.575911 4.2:+20766689 None:intergenic
CTGCACATGAAGCGGACACG+TGG 0.576780 4.2:+20767768 None:intergenic
GGGGATGAGCTGCAGAGAGG+TGG 0.577528 4.2:-20766518 MS.gene28675:CDS
GGTTCAACCTGGGAATGTAC+CGG 0.579359 4.2:-20766807 MS.gene28675:CDS
CCATCGTGAACCACCGTGGT+AGG 0.583743 4.2:+20766626 None:intergenic
CCGGTTCATATGCAAGGACA+TGG 0.585570 4.2:-20766788 MS.gene28675:CDS
CGGGTCAAGTGTATGGTGCG+GGG 0.586868 4.2:-20766682 MS.gene28675:CDS
AGAGAGTTTGTGGACAAGAG+CGG 0.587240 4.2:+20767647 None:intergenic
TTTACCACCAGGCGATGGCG+GGG 0.588535 4.2:-20766547 MS.gene28675:CDS
GAGCGGAGAAGGAGATGGAG+CGG 0.589074 4.2:+20767664 None:intergenic
GATTATCGGAGGGGCGAGGG+AGG 0.590762 4.2:+20767730 None:intergenic
CCAGAATAAGCACCTTCTTG+TGG 0.590779 4.2:+20766872 None:intergenic
GGAACGAGGGGAAGGAGAGA+CGG 0.593973 4.2:+20767812 None:intergenic
TGAGCTGCAGAGAGGTGGAA+CGG 0.596485 4.2:-20766513 MS.gene28675:CDS
ACCGATGAAGTAGAACAAAA+CGG 0.598459 4.2:+20767079 None:intergenic
AACCACCGTGGTAGGACTGT+AGG 0.600976 4.2:+20766634 None:intergenic
AATCAGCTCCGTTACGTCGG+CGG 0.603445 4.2:-20767711 MS.gene28675:CDS
TGGTGCGGGGATGAGGCATG+TGG 0.604646 4.2:-20766669 MS.gene28675:CDS
ATGATATGGTTGTTGAACAT+AGG 0.605177 4.2:+20766757 None:intergenic
CAAGCTGTGCAAAACAACTC+CGG 0.606932 4.2:-20766581 MS.gene28675:CDS
ACGGAATCGTCGTCTAAGTG+AGG 0.608186 4.2:+20767831 None:intergenic
AATGTACCGGTTCATATGCA+AGG 0.610445 4.2:-20766794 MS.gene28675:CDS
CGTTGAAGTGATTGGAACGA+GGG 0.612591 4.2:+20767799 None:intergenic
GGGTCAATTATGCGGCGAGT+TGG 0.613192 4.2:+20766481 None:intergenic
TCTCAACAACAACCACAAGA+AGG 0.617237 4.2:-20766884 MS.gene28675:CDS
TTCATGGATGCTTTAAACGG+TGG 0.619681 4.2:-20767301 MS.gene28675:CDS
GACGAAGACGAGCTCCTCGT+GGG 0.622004 4.2:+20767407 None:intergenic
TAACGGAGCTGATTATCGGA+GGG 0.629084 4.2:+20767720 None:intergenic
CACATGAAGCGGACACGTGG+AGG 0.629109 4.2:+20767771 None:intergenic
CGGAATCGTCGTCTAAGTGA+GGG 0.633230 4.2:+20767832 None:intergenic
AACGGAGCTGATTATCGGAG+GGG 0.640487 4.2:+20767721 None:intergenic
AGACGAAGACGAGCTCCTCG+TGG 0.640866 4.2:+20767406 None:intergenic
AAACACACGGGTCAATTATG+CGG 0.641392 4.2:+20766473 None:intergenic
GGCGTTTCGGGTTTAGAACG+AGG 0.645113 4.2:-20767280 MS.gene28675:CDS
CAGTCCCCGCCATCGCCTGG+TGG 0.647721 4.2:+20766543 None:intergenic
GATAATCAGCTCCGTTACGT+CGG 0.649927 4.2:-20767714 MS.gene28675:CDS
TCGTTGAAGTGATTGGAACG+AGG 0.653407 4.2:+20767798 None:intergenic
CGCGTTGATCACAGTAACAA+CGG 0.656379 4.2:-20767485 MS.gene28675:CDS
TGATACCCGATTGGCACCTG+AGG 0.660387 4.2:+20766722 None:intergenic
GTAACGGAGCTGATTATCGG+AGG 0.666207 4.2:+20767719 None:intergenic
CCTACCACGGTGGTTCACGA+TGG 0.666677 4.2:-20766626 MS.gene28675:CDS
AGGACTGTAGGGCTGAACCG+GGG 0.675018 4.2:+20766646 None:intergenic
GTTGAAGTGATTGGAACGAG+GGG 0.676892 4.2:+20767800 None:intergenic
GTTGTGGGTAGTGAAGTGCA+GGG 0.701572 4.2:+20766912 None:intergenic
GGTGGGGATGAGCTGCAGAG+AGG 0.703030 4.2:-20766521 MS.gene28675:CDS
TCAGCCCTACAGTCCTACCA+CGG 0.716211 4.2:-20766639 MS.gene28675:CDS
GCCCTACAGTCCTACCACGG+TGG 0.740967 4.2:-20766636 MS.gene28675:CDS
CAAACCATCGTGAACCACCG+TGG 0.762942 4.2:+20766622 None:intergenic
ACACAGAATGAAAACCCACG+AGG 0.829741 4.2:-20767421 MS.gene28675:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTATCCATTTTCAATTG+TGG + chr4.2:20767389-20767408 None:intergenic 20.0%
! AAATCAAACTGAAACTGAAA+CGG - chr4.2:20767355-20767374 MS.gene28675:CDS 25.0%
! ACAAGATAATGTTTCCAATT+CGG - chr4.2:20767193-20767212 MS.gene28675:CDS 25.0%
! ATCATGAATTGAAGAGATAA+GGG + chr4.2:20766767-20766786 None:intergenic 25.0%
AAGTACCTGATTATCTATTC+GGG - chr4.2:20767102-20767121 MS.gene28675:CDS 30.0%
AGTATCAGTTCACTCTTAAA+CGG - chr4.2:20766984-20767003 MS.gene28675:CDS 30.0%
ATGATATGGTTGTTGAACAT+AGG + chr4.2:20767576-20767595 None:intergenic 30.0%
GAACCCTGAATATAATAAAC+CGG + chr4.2:20767503-20767522 None:intergenic 30.0%
GATCATGAATTGAAGAGATA+AGG + chr4.2:20766768-20766787 None:intergenic 30.0%
GGAAACTTTCTTAGAGAAAA+GGG + chr4.2:20766746-20766765 None:intergenic 30.0%
GGTTTATTATATTCAGGGTT+CGG - chr4.2:20767502-20767521 MS.gene28675:CDS 30.0%
GTTCATCTAAAAGAGAAAAC+TGG - chr4.2:20767006-20767025 MS.gene28675:CDS 30.0%
GTTCCGGTTTATTATATTCA+GGG - chr4.2:20767497-20767516 MS.gene28675:CDS 30.0%
TAAAAACCTGTTCAGAAACT+TGG + chr4.2:20767331-20767350 None:intergenic 30.0%
TGAATATAATAAACCGGAAC+CGG + chr4.2:20767497-20767516 None:intergenic 30.0%
TGATATGGTTGTTGAACATA+GGG + chr4.2:20767575-20767594 None:intergenic 30.0%
TTTCTTAGAGAAAAGGGAAA+AGG + chr4.2:20766740-20766759 None:intergenic 30.0%
TTTGCAACCACAATTGAAAA+TGG - chr4.2:20767379-20767398 MS.gene28675:CDS 30.0%
! GAAGTACCTGATTATCTATT+CGG - chr4.2:20767101-20767120 MS.gene28675:CDS 30.0%
! GTTTCTGAACAGGTTTTTAA+AGG - chr4.2:20767332-20767351 MS.gene28675:CDS 30.0%
! TAAAAGAGAAAACTGGTTCA+TGG - chr4.2:20767013-20767032 MS.gene28675:CDS 30.0%
!!! TTATCAAGCTTGGTTTTAAC+CGG + chr4.2:20767308-20767327 None:intergenic 30.0%
AAGATCAGAAGCTTCTTCTA+TGG - chr4.2:20767073-20767092 MS.gene28675:CDS 35.0%
ACCGATGAAGTAGAACAAAA+CGG + chr4.2:20767254-20767273 None:intergenic 35.0%
AGAAACTTGGTTATCAAGCT+TGG + chr4.2:20767318-20767337 None:intergenic 35.0%
CACAAACTCTCTAAACTTTC+CGG - chr4.2:20766694-20766713 MS.gene28675:CDS 35.0%
GGGAAACTTTCTTAGAGAAA+AGG + chr4.2:20766747-20766766 None:intergenic 35.0%
GGTTCCGGTTTATTATATTC+AGG - chr4.2:20767496-20767515 MS.gene28675:CDS 35.0%
TAATGTTTCCAATTCGGATC+CGG - chr4.2:20767199-20767218 MS.gene28675:CDS 35.0%
TGATAACCAAGTTTCTGAAC+AGG - chr4.2:20767322-20767341 MS.gene28675:CDS 35.0%
TTGTTCTTTAGGTCGTGATT+CGG + chr4.2:20767150-20767169 None:intergenic 35.0%
! AGATGTCGTTGTTGTTCTTT+AGG + chr4.2:20767161-20767180 None:intergenic 35.0%
! ATCATTTGATGATACCCGAT+TGG + chr4.2:20767620-20767639 None:intergenic 35.0%
! TGGTTCATGGATGCTTTAAA+CGG - chr4.2:20767026-20767045 MS.gene28675:CDS 35.0%
!! ACCGTTTTGTTCTACTTCAT+CGG - chr4.2:20767250-20767269 MS.gene28675:CDS 35.0%
!!! GAGGGTTTTGAAATTCTTAC+CGG + chr4.2:20766716-20766735 None:intergenic 35.0%
AACGGTGAAGAAGAAACAAC+CGG + chr4.2:20767236-20767255 None:intergenic 40.0%
AATGTTTCCAATTCGGATCC+GGG - chr4.2:20767200-20767219 MS.gene28675:CDS 40.0%
ACCTGATTATCTATTCGGGT+TGG - chr4.2:20767106-20767125 MS.gene28675:CDS 40.0%
AGAGAAAAGGGAAAAGGTGA+GGG + chr4.2:20766734-20766753 None:intergenic 40.0%
CGGAAAGTTTAGAGAGTTTG+TGG + chr4.2:20766696-20766715 None:intergenic 40.0%
CGGGTATCATCAAATGATTC+CGG - chr4.2:20767622-20767641 MS.gene28675:intron 40.0%
GGGTATCATCAAATGATTCC+GGG - chr4.2:20767623-20767642 MS.gene28675:intron 40.0%
GTTGTTGAGATTGTGGTTGT+GGG + chr4.2:20767436-20767455 None:intergenic 40.0%
TAGAGAAAAGGGAAAAGGTG+AGG + chr4.2:20766735-20766754 None:intergenic 40.0%
TCATCAAATGATTCCGGGTA+CGG - chr4.2:20767628-20767647 MS.gene28675:intron 40.0%
TCCAACCCGAATAGATAATC+AGG + chr4.2:20767110-20767129 None:intergenic 40.0%
TCTCAACAACAACCACAAGA+AGG - chr4.2:20767446-20767465 MS.gene28675:CDS 40.0%
TGTTCTTTAGGTCGTGATTC+GGG + chr4.2:20767149-20767168 None:intergenic 40.0%
TGTTGTTGAGATTGTGGTTG+TGG + chr4.2:20767437-20767456 None:intergenic 40.0%
TTCAATTCATGATCGTGTGC+AGG - chr4.2:20766774-20766793 MS.gene28675:CDS 40.0%
TTCGTGAATCTTCTCCTTCT+GGG + chr4.2:20766959-20766978 None:intergenic 40.0%
TTCTATTCCGAATCTTCCAC+CGG - chr4.2:20767286-20767305 MS.gene28675:CDS 40.0%
! TGTGGTTGTTGTTGAGATTG+TGG + chr4.2:20767443-20767462 None:intergenic 40.0%
! TTCATGGATGCTTTAAACGG+TGG - chr4.2:20767029-20767048 MS.gene28675:CDS 40.0%
!! AATGTACCGGTTCATATGCA+AGG - chr4.2:20767536-20767555 MS.gene28675:CDS 40.0%
!! GTTTTAACCGGTGGAAGATT+CGG + chr4.2:20767296-20767315 None:intergenic 40.0%
AACGCGTCTAAGTCTTCGTT+TGG + chr4.2:20766831-20766850 None:intergenic 45.0%
ACACAGAATGAAAACCCACG+AGG - chr4.2:20766909-20766928 MS.gene28675:CDS 45.0%
AGAGAGTTTGTGGACAAGAG+CGG + chr4.2:20766686-20766705 None:intergenic 45.0%
AGTGAAGTGCAGGGTTAATC+TGG + chr4.2:20767412-20767431 None:intergenic 45.0%
ATATGAACCGGTACATTCCC+AGG + chr4.2:20767533-20767552 None:intergenic 45.0%
CAAGCTGTGCAAAACAACTC+CGG - chr4.2:20767749-20767768 MS.gene28675:CDS 45.0%
CATCAAATGATTCCGGGTAC+GGG - chr4.2:20767629-20767648 MS.gene28675:intron 45.0%
CCAGAATAAGCACCTTCTTG+TGG + chr4.2:20767461-20767480 None:intergenic 45.0%
CCATGTCCTTGCATATGAAC+CGG + chr4.2:20767545-20767564 None:intergenic 45.0%
CGTTGAAGTGATTGGAACGA+GGG + chr4.2:20766534-20766553 None:intergenic 45.0%
CTTCGTGAATCTTCTCCTTC+TGG + chr4.2:20766960-20766979 None:intergenic 45.0%
GATAATCAGCTCCGTTACGT+CGG - chr4.2:20766616-20766635 MS.gene28675:CDS 45.0%
GGTGGAAGATTCGGAATAGA+AGG + chr4.2:20767287-20767306 None:intergenic 45.0%
GGTTGCATCACTGGATGATA+TGG + chr4.2:20767590-20767609 None:intergenic 45.0%
GTTGAAGTGATTGGAACGAG+GGG + chr4.2:20766533-20766552 None:intergenic 45.0%
TAACGGAGCTGATTATCGGA+GGG + chr4.2:20766613-20766632 None:intergenic 45.0%
TCGTTGAAGTGATTGGAACG+AGG + chr4.2:20766535-20766554 None:intergenic 45.0%
TTCTGGTCATCATGCACAAC+CGG - chr4.2:20767475-20767494 MS.gene28675:CDS 45.0%
! AATCTTCTCCTTCTGGGAAG+AGG + chr4.2:20766953-20766972 None:intergenic 45.0%
! CGCGTTGATCACAGTAACAA+CGG - chr4.2:20766845-20766864 MS.gene28675:CDS 45.0%
! GACGTAACGGAGCTGATTAT+CGG + chr4.2:20766617-20766636 None:intergenic 45.0%
! GGTTTTCATTCTGTGTGACG+CGG + chr4.2:20766905-20766924 None:intergenic 45.0%
! TGCTTTAAACGGTGGCGTTT+CGG - chr4.2:20767037-20767056 MS.gene28675:CDS 45.0%
!! CCACAAGAAGGTGCTTATTC+TGG - chr4.2:20767458-20767477 MS.gene28675:CDS 45.0%
!!! TCAAGCTTGGTTTTAACCGG+TGG + chr4.2:20767305-20767324 None:intergenic 45.0%
AAACAACTCCGGTCCAGTTC+CGG - chr4.2:20767760-20767779 MS.gene28675:CDS 50.0%
AACGGAGCTGATTATCGGAG+GGG + chr4.2:20766612-20766631 None:intergenic 50.0%
AAGTGATTGGAACGAGGGGA+AGG + chr4.2:20766529-20766548 None:intergenic 50.0%
ACGGAATCGTCGTCTAAGTG+AGG + chr4.2:20766502-20766521 None:intergenic 50.0%
ATGCAACCTCAGGTGCCAAT+CGG - chr4.2:20767602-20767621 MS.gene28675:intron 50.0%
CCGGTTCATATGCAAGGACA+TGG - chr4.2:20767542-20767561 MS.gene28675:intron 50.0%
CGGAATCGTCGTCTAAGTGA+GGG + chr4.2:20766501-20766520 None:intergenic 50.0%
GAAGAAGAAACAACCGGAGC+AGG + chr4.2:20767230-20767249 None:intergenic 50.0%
GGTTCAACCTGGGAATGTAC+CGG - chr4.2:20767523-20767542 MS.gene28675:CDS 50.0%
GTCCGCTTCATGTGCAGTTT+CGG - chr4.2:20766568-20766587 MS.gene28675:CDS 50.0%
GTTGTGGGTAGTGAAGTGCA+GGG + chr4.2:20767421-20767440 None:intergenic 50.0%
TACGGGTCAAGTGTATGGTG+CGG - chr4.2:20767646-20767665 MS.gene28675:CDS 50.0%
TCATCCAGTGATGCAACCTC+AGG - chr4.2:20767592-20767611 MS.gene28675:intron 50.0%
TTTGTGGACAAGAGCGGAGA+AGG + chr4.2:20766680-20766699 None:intergenic 50.0%
! ATTCAGGGTTCGGTTCAACC+TGG - chr4.2:20767512-20767531 MS.gene28675:CDS 50.0%
! GCTTTAAACGGTGGCGTTTC+GGG - chr4.2:20767038-20767057 MS.gene28675:CDS 50.0%
! GTAACGGAGCTGATTATCGG+AGG + chr4.2:20766614-20766633 None:intergenic 50.0%
! TCATCATGCACAACCGGTTC+CGG - chr4.2:20767481-20767500 MS.gene28675:CDS 50.0%
! TTCAGGGTTCGGTTCAACCT+GGG - chr4.2:20767513-20767532 MS.gene28675:CDS 50.0%
!! AGGAGCGTCGTTGAAGTGAT+TGG + chr4.2:20766542-20766561 None:intergenic 50.0%
AACCACCGTGGTAGGACTGT+AGG + chr4.2:20767699-20767718 None:intergenic 55.0%
AAGTGTATGGTGCGGGGATG+AGG - chr4.2:20767654-20767673 MS.gene28675:CDS 55.0%
AATCAGCTCCGTTACGTCGG+CGG - chr4.2:20766619-20766638 MS.gene28675:CDS 55.0%
ACCACCGTGGTAGGACTGTA+GGG + chr4.2:20767698-20767717 None:intergenic 55.0%
ACGGGTCAAGTGTATGGTGC+GGG - chr4.2:20767647-20767666 MS.gene28675:CDS 55.0%
AGGAGAACCCGGATCCGAAT+TGG + chr4.2:20767210-20767229 None:intergenic 55.0%
CAAACCATCGTGAACCACCG+TGG + chr4.2:20767711-20767730 None:intergenic 55.0%
CCATACACTTGACCCGTACC+CGG + chr4.2:20767644-20767663 None:intergenic 55.0%
CGCCGAAACTGCACATGAAG+CGG + chr4.2:20766573-20766592 None:intergenic 55.0%
CGCTTCATGTGCAGTTTCGG+CGG - chr4.2:20766571-20766590 MS.gene28675:CDS 55.0%
CGTCTCTTCCTCTTCCCAGA+AGG - chr4.2:20766942-20766961 MS.gene28675:CDS 55.0%
GAAGGAGATGGAGCGGTTGA+CGG + chr4.2:20766662-20766681 None:intergenic 55.0%
GACAAGAGCGGAGAAGGAGA+TGG + chr4.2:20766674-20766693 None:intergenic 55.0%
GGTTGTGGGTAGTGAAGTGC+AGG + chr4.2:20767422-20767441 None:intergenic 55.0%
GTAGGACTGTAGGGCTGAAC+CGG + chr4.2:20767689-20767708 None:intergenic 55.0%
GTGGTAAACCGGAACTGGAC+CGG + chr4.2:20767771-20767790 None:intergenic 55.0%
TAGGACTGTAGGGCTGAACC+GGG + chr4.2:20767688-20767707 None:intergenic 55.0%
TCAGCCCTACAGTCCTACCA+CGG - chr4.2:20767691-20767710 MS.gene28675:CDS 55.0%
TCCAGTTCCGGTTTACCACC+AGG - chr4.2:20767772-20767791 MS.gene28675:CDS 55.0%
TGAGCTGCAGAGAGGTGGAA+CGG - chr4.2:20767817-20767836 MS.gene28675:CDS 55.0%
TGATACCCGATTGGCACCTG+AGG + chr4.2:20767611-20767630 None:intergenic 55.0%
TGCAACCTCAGGTGCCAATC+GGG - chr4.2:20767603-20767622 MS.gene28675:intron 55.0%
!! GGCGTTTCGGGTTTAGAACG+AGG - chr4.2:20767050-20767069 MS.gene28675:CDS 55.0%
ACAACCGGAGCAGGAGAACC+CGG + chr4.2:20767221-20767240 None:intergenic 60.0%
AGACGAAGACGAGCTCCTCG+TGG + chr4.2:20766927-20766946 None:intergenic 60.0%
AGCGGTTGACGGCGACGATA+CGG + chr4.2:20766651-20766670 None:intergenic 60.0%
AGCTGATTATCGGAGGGGCG+AGG + chr4.2:20766607-20766626 None:intergenic 60.0%
AGGACTGTAGGGCTGAACCG+GGG + chr4.2:20767687-20767706 None:intergenic 60.0%
CACATGAAGCGGACACGTGG+AGG + chr4.2:20766562-20766581 None:intergenic 60.0%
CCATCGTGAACCACCGTGGT+AGG + chr4.2:20767707-20767726 None:intergenic 60.0%
CCGGGTACGGGTCAAGTGTA+TGG - chr4.2:20767641-20767660 MS.gene28675:CDS 60.0%
CCTACCACGGTGGTTCACGA+TGG - chr4.2:20767704-20767723 MS.gene28675:CDS 60.0%
CGGGTCAAGTGTATGGTGCG+GGG - chr4.2:20767648-20767667 MS.gene28675:CDS 60.0%
CTGCACATGAAGCGGACACG+TGG + chr4.2:20766565-20766584 None:intergenic 60.0%
GACGAAGACGAGCTCCTCGT+GGG + chr4.2:20766926-20766945 None:intergenic 60.0%
GAGCGGAGAAGGAGATGGAG+CGG + chr4.2:20766669-20766688 None:intergenic 60.0%
GAGCTGCAGAGAGGTGGAAC+GGG - chr4.2:20767818-20767837 MS.gene28675:CDS 60.0%
GCCATCGCCTGGTGGTAAAC+CGG + chr4.2:20767782-20767801 None:intergenic 60.0%
GCCTGGTGGTAAACCGGAAC+TGG + chr4.2:20767776-20767795 None:intergenic 60.0%
GCTGATTATCGGAGGGGCGA+GGG + chr4.2:20766606-20766625 None:intergenic 60.0%
GGAACGAGGGGAAGGAGAGA+CGG + chr4.2:20766521-20766540 None:intergenic 60.0%
GGCACCTGAGGTTGCATCAC+TGG + chr4.2:20767599-20767618 None:intergenic 60.0%
GGTTTACCACCAGGCGATGG+CGG - chr4.2:20767781-20767800 MS.gene28675:CDS 60.0%
GTTTACCACCAGGCGATGGC+GGG - chr4.2:20767782-20767801 MS.gene28675:CDS 60.0%
TCCGGTTTACCACCAGGCGA+TGG - chr4.2:20767778-20767797 MS.gene28675:CDS 60.0%
TGCAGAGAGGTGGAACGGGT+CGG - chr4.2:20767822-20767841 MS.gene28675:CDS 60.0%
TTTACCACCAGGCGATGGCG+GGG - chr4.2:20767783-20767802 MS.gene28675:CDS 60.0%
CAGAGAGGTGGAACGGGTCG+GGG - chr4.2:20767824-20767843 MS.gene28675:CDS 65.0%
GATTATCGGAGGGGCGAGGG+AGG + chr4.2:20766603-20766622 None:intergenic 65.0%
GCAGAGAGGTGGAACGGGTC+GGG - chr4.2:20767823-20767842 MS.gene28675:CDS 65.0%
GCCCTACAGTCCTACCACGG+TGG - chr4.2:20767694-20767713 MS.gene28675:CDS 65.0%
GCGGTTGACGGCGACGATAC+GGG + chr4.2:20766650-20766669 None:intergenic 65.0%
GGATCCGGGTTCTCCTGCTC+CGG - chr4.2:20767214-20767233 MS.gene28675:CDS 65.0%
GGGGATGAGCTGCAGAGAGG+TGG - chr4.2:20767812-20767831 MS.gene28675:CDS 65.0%
GGTGGGGATGAGCTGCAGAG+AGG - chr4.2:20767809-20767828 MS.gene28675:CDS 65.0%
TGGTGCGGGGATGAGGCATG+TGG - chr4.2:20767661-20767680 MS.gene28675:CDS 65.0%
GGGGATGAGGCATGTGGCCC+CGG - chr4.2:20767667-20767686 MS.gene28675:CDS 70.0%
! GGGTGTCGCCGCCGACGTAA+CGG + chr4.2:20766630-20766649 None:intergenic 70.0%
!! AGGCGATGGCGGGGACTGGT+GGG - chr4.2:20767792-20767811 MS.gene28675:CDS 70.0%
CACCAGTCCCCGCCATCGCC+TGG + chr4.2:20767793-20767812 None:intergenic 75.0%
CAGTCCCCGCCATCGCCTGG+TGG + chr4.2:20767790-20767809 None:intergenic 75.0%
!! CACCAGGCGATGGCGGGGAC+TGG - chr4.2:20767788-20767807 MS.gene28675:CDS 75.0%
!! CAGGCGATGGCGGGGACTGG+TGG - chr4.2:20767791-20767810 MS.gene28675:CDS 75.0%
!! GGCGATGGCGGGGACTGGTG+GGG - chr4.2:20767793-20767812 MS.gene28675:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 20766484 20767868 20766484 ID=MS.gene28675
chr4.2 mRNA 20766484 20767868 20766484 ID=MS.gene28675.t1;Parent=MS.gene28675
chr4.2 exon 20767637 20767868 20767637 ID=MS.gene28675.t1.exon1;Parent=MS.gene28675.t1
chr4.2 CDS 20767637 20767868 20767637 ID=cds.MS.gene28675.t1;Parent=MS.gene28675.t1
chr4.2 exon 20766484 20767556 20766484 ID=MS.gene28675.t1.exon2;Parent=MS.gene28675.t1
chr4.2 CDS 20766484 20767556 20766484 ID=cds.MS.gene28675.t1;Parent=MS.gene28675.t1
Gene Sequence

>MS.gene28675

ATGGCGTCGTCACTCCCTCACTTAGACGACGATTCCGTCTCTCCTTCCCCTCGTTCCAATCACTTCAACGACGCTCCTCCACGTGTCCGCTTCATGTGCAGTTTCGGCGGCAAAATCCTCCCTCGCCCCTCCGATAATCAGCTCCGTTACGTCGGCGGCGACACCCGTATCGTCGCCGTCAACCGCTCCATCTCCTTCTCCGCTCTTGTCCACAAACTCTCTAAACTTTCCGGTAAGAATTTCAAAACCCTCACCTTTTCCCTTTTCTCTAAGAAAGTTTCCCTTATCTCTTCAATTCATGATCGTGTGCAGGTATGAGCAACATAACAGCGAAGTATCAACTACCAAACGAAGACTTAGACGCGTTGATCACAGTAACAACGGACGAAGACGTAGATAACATGATAGACGAATACGACCGCGTCACACAGAATGAAAACCCACGAGGAGCTCGTCTTCGTCTCTTCCTCTTCCCAGAAGGAGAAGATTCACGAAGCAACAGTATCAGTTCACTCTTAAACGGTTCATCTAAAAGAGAAAACTGGTTCATGGATGCTTTAAACGGTGGCGTTTCGGGTTTAGAACGAGGAAGATCAGAAGCTTCTTCTATGGTTTCTGAAGTACCTGATTATCTATTCGGGTTGGATAATAACTCTGAAGTACCCGAATCACGACCTAAAGAACAACAACGACATCTTCTTCAACAACAACAAGATAATGTTTCCAATTCGGATCCGGGTTCTCCTGCTCCGGTTGTTTCTTCTTCACCGTTTTGTTCTACTTCATCGGTGTTGTCTGTGCCTTCTATTCCGAATCTTCCACCGGTTAAAACCAAGCTTGATAACCAAGTTTCTGAACAGGTTTTTAAAGGAAATCAAACTGAAACTGAAACGGTTTTGCAACCACAATTGAAAATGGATAATTACCAGATTAACCCTGCACTTCACTACCCACAACCACAATCTCAACAACAACCACAAGAAGGTGCTTATTCTGGTCATCATGCACAACCGGTTCCGGTTTATTATATTCAGGGTTCGGTTCAACCTGGGAATGTACCGGTTCATATGCAAGGACATGGACATTATCCCTATGTTCAACAACCATATCATCCAGTGATGCAACCTCAGGTGCCAATCGGGTATCATCAAATGATTCCGGGTACGGGTCAAGTGTATGGTGCGGGGATGAGGCATGTGGCCCCGGTTCAGCCCTACAGTCCTACCACGGTGGTTCACGATGGTTTGAAACAACAGCAAGTGTTTCAAGCTGTGCAAAACAACTCCGGTCCAGTTCCGGTTTACCACCAGGCGATGGCGGGGACTGGTGGGGATGAGCTGCAGAGAGGTGGAACGGGTCGGGGCACCAACTCGCCGCATAATTGA
Protein sequence

>MS.gene28675.t1

MASSLPHLDDDSVSPSPRSNHFNDAPPRVRFMCSFGGKILPRPSDNQLRYVGGDTRIVAVNRSISFSALVHKLSKLSGMSNITAKYQLPNEDLDALITVTTDEDVDNMIDEYDRVTQNENPRGARLRLFLFPEGEDSRSNSISSLLNGSSKRENWFMDALNGGVSGLERGRSEASSMVSEVPDYLFGLDNNSEVPESRPKEQQRHLLQQQQDNVSNSDPGSPAPVVSSSPFCSTSSVLSVPSIPNLPPVKTKLDNQVSEQVFKGNQTETETVLQPQLKMDNYQINPALHYPQPQSQQQPQEGAYSGHHAQPVPVYYIQGSVQPGNVPVHMQGHGHYPYVQQPYHPVMQPQVPIGYHQMIPGTGQVYGAGMRHVAPVQPYSPTTVVHDGLKQQQVFQAVQNNSGPVPVYHQAMAGTGGDELQRGGTGRGTNSPHN