AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056631


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056631.t1 MTR_4g068710 98.148 54 1 0 1 54 1 54 4.71e-35 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056631.t1 AT1G49410 61.111 54 21 0 1 54 1 54 2.64e-23 82.8

Find 12 sgRNAs with CRISPR-Local

Find 13 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATCTCATGTCGCCATGTT+TGG 0.332693 4.3:+36486948 MS.gene056631:CDS
TTCAAAGCCACAGCCTTGTC+TGG 0.381218 4.3:-36486918 None:intergenic
TTGAGAAAATGAAGAATGTA+AGG 0.435892 4.3:-36486999 None:intergenic
AAGGTAGCGATCATGTTTCC+AGG 0.437874 4.3:+36486879 None:intergenic
ATGTCGCCATGTTTGGAACA+TGG 0.492135 4.3:+36486955 MS.gene056631:CDS
TGTCGCCATGTTTGGAACAT+GGG 0.532708 4.3:+36486956 MS.gene056631:CDS
GCTTGAGTTCCTCGTTCTCG+CGG 0.558292 4.3:-36487022 None:intergenic
TGAGAAAATGAAGAATGTAA+GGG 0.583076 4.3:-36486998 None:intergenic
GAGAAAATGAAGAATGTAAG+GGG 0.607716 4.3:-36486997 None:intergenic
GCGAAAACCAGACAAGGCTG+TGG 0.631734 4.3:+36486911 MS.gene056631:CDS
AAGAACCCATGTTCCAAACA+TGG 0.637331 4.3:-36486961 None:intergenic
GTTCATGCGAAAACCAGACA+AGG 0.672633 4.3:+36486905 MS.gene056631:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGAGAAAATGAAGAATGTAA+GGG - chr4.3:36487001-36487020 None:intergenic 25.0%
! TTGAGAAAATGAAGAATGTA+AGG - chr4.3:36487002-36487021 None:intergenic 25.0%
GAGAAAATGAAGAATGTAAG+GGG - chr4.3:36487000-36487019 None:intergenic 30.0%
AAATCTCATGTCGCCATGTT+TGG + chr4.3:36486948-36486967 MS.gene056631:CDS 40.0%
AAGAACCCATGTTCCAAACA+TGG - chr4.3:36486964-36486983 None:intergenic 40.0%
GTTCATGCGAAAACCAGACA+AGG + chr4.3:36486905-36486924 MS.gene056631:CDS 45.0%
! ATGTCGCCATGTTTGGAACA+TGG + chr4.3:36486955-36486974 MS.gene056631:CDS 45.0%
! GGTTTTCGCATGAACATTCC+TGG - chr4.3:36486900-36486919 None:intergenic 45.0%
! TGTCGCCATGTTTGGAACAT+GGG + chr4.3:36486956-36486975 MS.gene056631:CDS 45.0%
! TTCAAAGCCACAGCCTTGTC+TGG - chr4.3:36486921-36486940 None:intergenic 50.0%
! TTTTCTCAACCGCGAGAACG+AGG + chr4.3:36487013-36487032 MS.gene056631:CDS 50.0%
GCGAAAACCAGACAAGGCTG+TGG + chr4.3:36486911-36486930 MS.gene056631:CDS 55.0%
GCTTGAGTTCCTCGTTCTCG+CGG - chr4.3:36487025-36487044 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 36486891 36487055 36486891 ID=MS.gene056631
chr4.3 mRNA 36486891 36487055 36486891 ID=MS.gene056631.t1;Parent=MS.gene056631
chr4.3 exon 36486891 36487055 36486891 ID=MS.gene056631.t1.exon1;Parent=MS.gene056631.t1
chr4.3 CDS 36486891 36487055 36486891 ID=cds.MS.gene056631.t1;Parent=MS.gene056631.t1
Gene Sequence

>MS.gene056631

ATGTTTCCAGGAATGTTCATGCGAAAACCAGACAAGGCTGTGGCTTTGAAGCAGCTCAAATCTCATGTCGCCATGTTTGGAACATGGGTTCTTGTTATTCGTGTCACCCCTTACATTCTTCATTTTCTCAACCGCGAGAACGAGGAACTCAAGCTTGAGCTCTAG

Protein sequence

>MS.gene056631.t1

MFPGMFMRKPDKAVALKQLKSHVAMFGTWVLVIRVTPYILHFLNRENEELKLEL