AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene29213


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29213.t1 MTR_0365s0010 95.814 215 9 0 16 230 153 367 4.93e-147 417
MS.gene29213.t1 MTR_0400s0020 96.894 161 5 0 70 230 1 161 8.74e-112 320
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29213.t1 AT3G19990 64.286 224 74 4 16 235 148 369 2.01e-93 280
MS.gene29213.t1 AT3G19990 64.286 224 74 4 16 235 173 394 5.17e-93 280

Find 56 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAAATGTTAGGAGTAAA+AGG 0.193080 1.1:-23781606 MS.gene29213:CDS
GATGGTAGACTTGAGTTTAA+AGG 0.227505 1.1:-23781753 MS.gene29213:CDS
CGATAAATGTACCAAGGTTT+TGG 0.239302 1.1:-23781523 MS.gene29213:CDS
GAGTTTAAAGGGAACAATTT+TGG 0.292010 1.1:-23781741 MS.gene29213:CDS
TTAGGAGTAAAAGGAGTTAA+TGG 0.324776 1.1:-23781597 MS.gene29213:CDS
CAAGGTCTTGTTCCTGCTGA+TGG 0.365492 1.1:-23781912 MS.gene29213:CDS
ATGGTAGACTTGAGTTTAAA+GGG 0.374370 1.1:-23781752 MS.gene29213:CDS
GAAGAAAAGAAGAAAATGTT+AGG 0.378990 1.1:-23781615 MS.gene29213:CDS
CTTGTTACGGAGGAAGGGTT+TGG 0.384705 1.1:+23782235 None:intergenic
ATGATTTGCGAAGAAGGATT+GGG 0.392847 1.1:-23781456 MS.gene29213:CDS
AGTCAAGTTTATTTGACAAA+AGG 0.397768 1.1:-23781363 MS.gene29213:CDS
GGGGAAGATTGAGGAGTTGT+TGG 0.413719 1.1:-23781436 MS.gene29213:CDS
ACTTGACTCAACACCACATA+TGG 0.426604 1.1:+23781378 None:intergenic
GTCAAGTTTATTTGACAAAA+GGG 0.433282 1.1:-23781362 MS.gene29213:CDS
TATGATTTGCGAAGAAGGAT+TGG 0.442913 1.1:-23781457 MS.gene29213:CDS
AGTTGCAAGAGATTTGTATT+GGG 0.443766 1.1:-23781484 MS.gene29213:CDS
GGAAATCAGAATTGTCTTTG+AGG 0.445520 1.1:-23781989 MS.gene29213:intron
TTGGTACATTTATCGAAAAC+CGG 0.448739 1.1:+23781531 None:intergenic
ACATATGGCTCTCCCACAAA+AGG 0.449156 1.1:+23781393 None:intergenic
GGTGCTGTTTGGCCTGGAAA+TGG 0.450409 1.1:-23781720 MS.gene29213:CDS
TAGTTGCAAGAGATTTGTAT+TGG 0.453285 1.1:-23781485 MS.gene29213:CDS
GAAATCAGAATTGTCTTTGA+GGG 0.456029 1.1:-23781988 MS.gene29213:intron
TTTGAGGGCTGCAAAGGAAA+AGG 0.459072 1.1:-23781973 MS.gene29213:CDS
AGTGAGCTTGTTACGGAGGA+AGG 0.459668 1.1:+23782229 None:intergenic
AAACCCGGCTTACCATTTCC+AGG 0.460541 1.1:+23781708 None:intergenic
ATGTTGTTTGAGAACTTTGA+TGG 0.465122 1.1:-23781771 MS.gene29213:CDS
AGAAGGATTGGGGAAGATTG+AGG 0.469108 1.1:-23781445 MS.gene29213:CDS
CTCAATATGTTCATCTCTCT+CGG 0.471671 1.1:+23781566 None:intergenic
GGAAAAGGGGATATTTGATA+AGG 0.478145 1.1:-23781958 MS.gene29213:CDS
GTTGAATTCAATTTCGAGGA+TGG 0.480600 1.1:-23781682 MS.gene29213:CDS
GAGAGATGAACATATTGAGT+TGG 0.498516 1.1:-23781562 MS.gene29213:CDS
GTGAAGACGTGAAGCTTTCG+AGG 0.504729 1.1:-23781863 MS.gene29213:CDS
TTGAGGGCTGCAAAGGAAAA+GGG 0.514403 1.1:-23781972 MS.gene29213:CDS
AAGGTCTTGTTCCTGCTGAT+GGG 0.516621 1.1:-23781911 MS.gene29213:CDS
TGTGGTTGAATTCAATTTCG+AGG 0.520872 1.1:-23781686 MS.gene29213:CDS
ATTGTCTTTGAGGGCTGCAA+AGG 0.521643 1.1:-23781979 MS.gene29213:CDS
GAAGCTATGATTTGCGAAGA+AGG 0.526042 1.1:-23781462 MS.gene29213:CDS
TTGAATTCAATTTCGAGGAT+GGG 0.526074 1.1:-23781681 MS.gene29213:CDS
TGGCCTGGAAATGGTAAGCC+GGG 0.526760 1.1:-23781711 MS.gene29213:CDS
AAAGTGAAGCATATTCGTAC+AGG 0.545228 1.1:-23781885 MS.gene29213:CDS
TTGGCCTGGAAATGGTAAGC+CGG 0.547055 1.1:-23781712 MS.gene29213:CDS
ATCCCCTGCATCCAAAACCT+TGG 0.551710 1.1:+23781512 None:intergenic
TTGATAAGGATGAAAGTTCG+AGG 0.559149 1.1:-23781944 MS.gene29213:CDS
ATTGAATTCAACCACAAACC+CGG 0.559263 1.1:+23781693 None:intergenic
CATATGGCTCTCCCACAAAA+GGG 0.560481 1.1:+23781394 None:intergenic
GTGAGCTTGTTACGGAGGAA+GGG 0.580144 1.1:+23782230 None:intergenic
TTATAATTTGATTCTGAGAG+AGG 0.581262 1.1:-23781652 MS.gene29213:CDS
GAAATGGTAAGCCGGGTTTG+TGG 0.595506 1.1:-23781704 MS.gene29213:CDS
TGACAAAAGGGAGAAAGAAG+CGG 0.619200 1.1:-23781350 MS.gene29213:CDS
TGATTTGCGAAGAAGGATTG+GGG 0.639426 1.1:-23781455 MS.gene29213:CDS
TGAGGGCTGCAAAGGAAAAG+GGG 0.639513 1.1:-23781971 MS.gene29213:CDS
AGGGAGAAAGAAGCGGAGAG+AGG 0.639645 1.1:-23781343 MS.gene29213:CDS
AGTTCGAGGAAGAAATTGCA+AGG 0.642974 1.1:-23781930 MS.gene29213:CDS
GCTTTCGAGGAGAGTTGTCG+CGG 0.664379 1.1:-23781850 MS.gene29213:CDS
TTGAACTTGATAACGTGACA+TGG 0.686592 1.1:+23782268 None:intergenic
GTACATTTATCGAAAACCGG+TGG 0.694188 1.1:+23781534 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAGAAAATGTTAGGAGTAAA+AGG - chr1.1:23781998-23782017 MS.gene29213:intron 25.0%
! AGTCAAGTTTATTTGACAAA+AGG - chr1.1:23782241-23782260 MS.gene29213:intron 25.0%
! GAAGAAAAGAAGAAAATGTT+AGG - chr1.1:23781989-23782008 MS.gene29213:intron 25.0%
! GTCAAGTTTATTTGACAAAA+GGG - chr1.1:23782242-23782261 MS.gene29213:intron 25.0%
!! TTATAATTTGATTCTGAGAG+AGG - chr1.1:23781952-23781971 MS.gene29213:CDS 25.0%
ACAATTCTGATTTCCAAAGA+TGG + chr1.1:23781610-23781629 None:intergenic 30.0%
AGTTGCAAGAGATTTGTATT+GGG - chr1.1:23782120-23782139 MS.gene29213:intron 30.0%
ATGGTAGACTTGAGTTTAAA+GGG - chr1.1:23781852-23781871 MS.gene29213:CDS 30.0%
GAAATCAGAATTGTCTTTGA+GGG - chr1.1:23781616-23781635 MS.gene29213:CDS 30.0%
TAGTTGCAAGAGATTTGTAT+TGG - chr1.1:23782119-23782138 MS.gene29213:intron 30.0%
TATCTTGAAAACCAAAAAGC+TGG + chr1.1:23781814-23781833 None:intergenic 30.0%
TTAGGAGTAAAAGGAGTTAA+TGG - chr1.1:23782007-23782026 MS.gene29213:intron 30.0%
TTGAATTCAATTTCGAGGAT+GGG - chr1.1:23781923-23781942 MS.gene29213:CDS 30.0%
TTGGTACATTTATCGAAAAC+CGG + chr1.1:23782076-23782095 None:intergenic 30.0%
! ATGTTGTTTGAGAACTTTGA+TGG - chr1.1:23781833-23781852 MS.gene29213:CDS 30.0%
! ATTGAGAAAAACCCTTTTGT+GGG - chr1.1:23782199-23782218 MS.gene29213:intron 30.0%
! GAGTTTAAAGGGAACAATTT+TGG - chr1.1:23781863-23781882 MS.gene29213:CDS 30.0%
! TATTGAGAAAAACCCTTTTG+TGG - chr1.1:23782198-23782217 MS.gene29213:intron 30.0%
!! GGTTTTTGTTATGATGACTA+TGG - chr1.1:23781775-23781794 MS.gene29213:CDS 30.0%
AAAGATAGCAAGGTAAACGT+AGG + chr1.1:23781580-23781599 None:intergenic 35.0%
AAAGTGAAGCATATTCGTAC+AGG - chr1.1:23781719-23781738 MS.gene29213:CDS 35.0%
ATCTTTGATCCTTCCATCTT+TGG - chr1.1:23781594-23781613 MS.gene29213:CDS 35.0%
ATTGAATTCAACCACAAACC+CGG + chr1.1:23781914-23781933 None:intergenic 35.0%
CTCAATATGTTCATCTCTCT+CGG + chr1.1:23782041-23782060 None:intergenic 35.0%
GAGAGATGAACATATTGAGT+TGG - chr1.1:23782042-23782061 MS.gene29213:intron 35.0%
GATGGTAGACTTGAGTTTAA+AGG - chr1.1:23781851-23781870 MS.gene29213:CDS 35.0%
GGAAAAGGGGATATTTGATA+AGG - chr1.1:23781646-23781665 MS.gene29213:CDS 35.0%
GGAAATCAGAATTGTCTTTG+AGG - chr1.1:23781615-23781634 MS.gene29213:CDS 35.0%
GGATGATTGTGATTAGGAAA+GGG + chr1.1:23781417-23781436 None:intergenic 35.0%
GTTGAATTCAATTTCGAGGA+TGG - chr1.1:23781922-23781941 MS.gene29213:CDS 35.0%
TCCATGTTTGTGCTAACATT+CGG + chr1.1:23781455-23781474 None:intergenic 35.0%
TGTGGTTGAATTCAATTTCG+AGG - chr1.1:23781918-23781937 MS.gene29213:CDS 35.0%
TTCTGATTTCCAAAGATGGA+AGG + chr1.1:23781606-23781625 None:intergenic 35.0%
TTGATAAGGATGAAAGTTCG+AGG - chr1.1:23781660-23781679 MS.gene29213:CDS 35.0%
! ATGATTTGCGAAGAAGGATT+GGG - chr1.1:23782148-23782167 MS.gene29213:intron 35.0%
! CGATAAATGTACCAAGGTTT+TGG - chr1.1:23782081-23782100 MS.gene29213:intron 35.0%
! GAGAGAGGAAGAGATATTTT+TGG - chr1.1:23781967-23781986 MS.gene29213:CDS 35.0%
! GATTTTTCTGACCAGCTTTT+TGG - chr1.1:23781800-23781819 MS.gene29213:CDS 35.0%
! GGTTTTCGATAAATGTACCA+AGG - chr1.1:23782075-23782094 MS.gene29213:intron 35.0%
! TATGATTTGCGAAGAAGGAT+TGG - chr1.1:23782147-23782166 MS.gene29213:intron 35.0%
!! AAATGTCGATAAGGTGTTGA+AGG + chr1.1:23781483-23781502 None:intergenic 35.0%
!!! TATTGAGTTGGTTTTACCAC+CGG - chr1.1:23782054-23782073 MS.gene29213:intron 35.0%
ACTTGACTCAACACCACATA+TGG + chr1.1:23782229-23782248 None:intergenic 40.0%
AGCAAGGTAAACGTAGGAAT+TGG + chr1.1:23781574-23781593 None:intergenic 40.0%
AGTGTGGAGATTTCCAAAGA+TGG + chr1.1:23781516-23781535 None:intergenic 40.0%
AGTTCGAGGAAGAAATTGCA+AGG - chr1.1:23781674-23781693 MS.gene29213:CDS 40.0%
ATTTCCAAAGATGGAGAGTG+TGG + chr1.1:23781507-23781526 None:intergenic 40.0%
CGGATGATTGTGATTAGGAA+AGG + chr1.1:23781418-23781437 None:intergenic 40.0%
GAGTGTGGAAAATGTCGATA+AGG + chr1.1:23781492-23781511 None:intergenic 40.0%
GTACATTTATCGAAAACCGG+TGG + chr1.1:23782073-23782092 None:intergenic 40.0%
TCCGAATGTTAGCACAAACA+TGG - chr1.1:23781451-23781470 MS.gene29213:CDS 40.0%
TGACAAAAGGGAGAAAGAAG+CGG - chr1.1:23782254-23782273 MS.gene29213:CDS 40.0%
TGGAAGGATCAAAGATAGCA+AGG + chr1.1:23781590-23781609 None:intergenic 40.0%
TTGTGATTAGGAAAGGGAGT+TGG + chr1.1:23781411-23781430 None:intergenic 40.0%
! GAAGCTATGATTTGCGAAGA+AGG - chr1.1:23782142-23782161 MS.gene29213:intron 40.0%
! TGATTTGCGAAGAAGGATTG+GGG - chr1.1:23782149-23782168 MS.gene29213:intron 40.0%
! TTTTCCACACTCTCCATCTT+TGG - chr1.1:23781500-23781519 MS.gene29213:CDS 40.0%
!! TGATGGAAGTGAGCTTGTTA+CGG + chr1.1:23781385-23781404 None:intergenic 40.0%
!! TTCACTTTTATCCCATCAGC+AGG + chr1.1:23781707-23781726 None:intergenic 40.0%
!!! GGAACAATTTTGGTGCTGTT+TGG - chr1.1:23781873-23781892 MS.gene29213:CDS 40.0%
ACATATGGCTCTCCCACAAA+AGG + chr1.1:23782214-23782233 None:intergenic 45.0%
AGAAGGATTGGGGAAGATTG+AGG - chr1.1:23782159-23782178 MS.gene29213:intron 45.0%
ATCACAATCATCCGTTCCGT+TGG - chr1.1:23781424-23781443 MS.gene29213:CDS 45.0%
CATATGGCTCTCCCACAAAA+GGG + chr1.1:23782213-23782232 None:intergenic 45.0%
CGGAACGGATGATTGTGATT+AGG + chr1.1:23781423-23781442 None:intergenic 45.0%
CTAACATTCGGATGCACCAA+CGG + chr1.1:23781443-23781462 None:intergenic 45.0%
GGAATAGGCTACAAGAGACA+CGG + chr1.1:23781539-23781558 None:intergenic 45.0%
TAGGAATTGGAGTGTGCTGA+GGG + chr1.1:23781561-23781580 None:intergenic 45.0%
TTGAGGGCTGCAAAGGAAAA+GGG - chr1.1:23781632-23781651 MS.gene29213:CDS 45.0%
TTTGAGGGCTGCAAAGGAAA+AGG - chr1.1:23781631-23781650 MS.gene29213:CDS 45.0%
! ATTGTCTTTGAGGGCTGCAA+AGG - chr1.1:23781625-23781644 MS.gene29213:CDS 45.0%
! TTTTGTGGGAGAGCCATATG+TGG - chr1.1:23782213-23782232 MS.gene29213:intron 45.0%
!! AAGGTCTTGTTCCTGCTGAT+GGG - chr1.1:23781693-23781712 MS.gene29213:CDS 45.0%
!!! AATTTTGGTGCTGTTTGGCC+TGG - chr1.1:23781878-23781897 MS.gene29213:CDS 45.0%
!!! GTACCAAGGTTTTGGATGCA+GGG - chr1.1:23782089-23782108 MS.gene29213:intron 45.0%
!!! TACCAAGGTTTTGGATGCAG+GGG - chr1.1:23782090-23782109 MS.gene29213:intron 45.0%
!!! TGTACCAAGGTTTTGGATGC+AGG - chr1.1:23782088-23782107 MS.gene29213:intron 45.0%
AAACCCGGCTTACCATTTCC+AGG + chr1.1:23781899-23781918 None:intergenic 50.0%
AGGAATTGGAGTGTGCTGAG+GGG + chr1.1:23781560-23781579 None:intergenic 50.0%
ATCCCCTGCATCCAAAACCT+TGG + chr1.1:23782095-23782114 None:intergenic 50.0%
ATTCGGATGCACCAACGGAA+CGG + chr1.1:23781438-23781457 None:intergenic 50.0%
CTTGTTACGGAGGAAGGGTT+TGG + chr1.1:23781372-23781391 None:intergenic 50.0%
GAAATGGTAAGCCGGGTTTG+TGG - chr1.1:23781900-23781919 MS.gene29213:CDS 50.0%
GGGGAAGATTGAGGAGTTGT+TGG - chr1.1:23782168-23782187 MS.gene29213:intron 50.0%
GTAGGAATTGGAGTGTGCTG+AGG + chr1.1:23781562-23781581 None:intergenic 50.0%
GTGAAGACGTGAAGCTTTCG+AGG - chr1.1:23781741-23781760 MS.gene29213:CDS 50.0%
TGAGGGCTGCAAAGGAAAAG+GGG - chr1.1:23781633-23781652 MS.gene29213:CDS 50.0%
TTGGCCTGGAAATGGTAAGC+CGG - chr1.1:23781892-23781911 MS.gene29213:CDS 50.0%
!! AGTGAGCTTGTTACGGAGGA+AGG + chr1.1:23781378-23781397 None:intergenic 50.0%
!! CAAGGTCTTGTTCCTGCTGA+TGG - chr1.1:23781692-23781711 MS.gene29213:CDS 50.0%
!! GGAAAGGGAGTTGGTGTTGA+TGG + chr1.1:23781402-23781421 None:intergenic 50.0%
!! GTGAGCTTGTTACGGAGGAA+GGG + chr1.1:23781377-23781396 None:intergenic 50.0%
!! TGGAAGTGAGCTTGTTACGG+AGG + chr1.1:23781382-23781401 None:intergenic 50.0%
AGGGAGAAAGAAGCGGAGAG+AGG - chr1.1:23782261-23782280 MS.gene29213:CDS 55.0%
GCTACAAGAGACACGGAGTG+TGG + chr1.1:23781532-23781551 None:intergenic 55.0%
GCTTTCGAGGAGAGTTGTCG+CGG - chr1.1:23781754-23781773 MS.gene29213:CDS 55.0%
TGGAGTGTGCTGAGGGGAAT+AGG + chr1.1:23781554-23781573 None:intergenic 55.0%
TGGCCTGGAAATGGTAAGCC+GGG - chr1.1:23781893-23781912 MS.gene29213:CDS 55.0%
!! GGTGCTGTTTGGCCTGGAAA+TGG - chr1.1:23781884-23781903 MS.gene29213:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 23781338 23782288 23781338 ID=MS.gene29213
chr1.1 mRNA 23781338 23782288 23781338 ID=MS.gene29213.t1;Parent=MS.gene29213
chr1.1 exon 23782243 23782288 23782243 ID=MS.gene29213.t1.exon1;Parent=MS.gene29213.t1
chr1.1 CDS 23782243 23782288 23782243 ID=cds.MS.gene29213.t1;Parent=MS.gene29213.t1
chr1.1 exon 23781338 23782002 23781338 ID=MS.gene29213.t1.exon2;Parent=MS.gene29213.t1
chr1.1 CDS 23781338 23782002 23781338 ID=cds.MS.gene29213.t1;Parent=MS.gene29213.t1
Gene Sequence

>MS.gene29213

ATGTCACGTTATCAAGTTCAAGTGAATACTTCCAAACCCTTCCTCCGTAACAAGCTCACTTCCATCAACACCAACTCCCTTTCCTAATCACAATCATCCGTTCCGTTGGTGCATCCGAATGTTAGCACAAACATGGAATTTTCCTTCAACACCTTATCGACATTTTCCACACTCTCCATCTTTGGAAATCTCCACACTCCGTGTCTCTTGTAGCCTATTCCCCTCAGCACACTCCAATTCCTACGTTTACCTTGCTATCTTTGATCCTTCCATCTTTGGAAATCAGAATTGTCTTTGAGGGCTGCAAAGGAAAAGGGGATATTTGATAAGGATGAAAGTTCGAGGAAGAAATTGCAAGGTCTTGTTCCTGCTGATGGGATAAAAGTGAAGCATATTCGTACAGGTGAAGACGTGAAGCTTTCGAGGAGAGTTGTCGCGGTTTTTGTTATGATGACTATGGCTGATTTTTCTGACCAGCTTTTTGGTTTTCAAGATATGTTGTTTGAGAACTTTGATGGTAGACTTGAGTTTAAAGGGAACAATTTTGGTGCTGTTTGGCCTGGAAATGGTAAGCCGGGTTTGTGGTTGAATTCAATTTCGAGGATGGGAGCTGTTTATAATTTGATTCTGAGAGAGGAAGAGATATTTTTGGAAGAAAAGAAGAAAATGTTAGGAGTAAAAGGAGTTAATGGTGTTGATTCCGAGAGAGATGAACATATTGAGTTGGTTTTACCACCGGTTTTCGATAAATGTACCAAGGTTTTGGATGCAGGGGATCAAATAGTTGCAAGAGATTTGTATTGGGAAGCTATGATTTGCGAAGAAGGATTGGGGAAGATTGAGGAGTTGTTGGTGAAGAGTATTGAGAAAAACCCTTTTGTGGGAGAGCCATATGTGGTGTTGAGTCAAGTTTATTTGACAAAAGGGAGAAAGAAGCGGAGAGAGGATTGA

Protein sequence

>MS.gene29213.t1

MSRYQVQVNTSKPFLQLSLRAAKEKGIFDKDESSRKKLQGLVPADGIKVKHIRTGEDVKLSRRVVAVFVMMTMADFSDQLFGFQDMLFENFDGRLEFKGNNFGAVWPGNGKPGLWLNSISRMGAVYNLILREEEIFLEEKKKMLGVKGVNGVDSERDEHIELVLPPVFDKCTKVLDAGDQIVARDLYWEAMICEEGLGKIEELLVKSIEKNPFVGEPYVVLSQVYLTKGRKKRRED