Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29692.t1 | XP_003616694.1 | 95.6 | 205 | 9 | 0 | 1 | 205 | 1 | 205 | 2.90E-107 | 397.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29692.t1 | O80920 | 72.7 | 165 | 44 | 1 | 39 | 203 | 32 | 195 | 1.6e-61 | 237.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29692.t1 | G7K772 | 95.6 | 205 | 9 | 0 | 1 | 205 | 1 | 205 | 2.1e-107 | 397.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052795 | MS.gene29692 | 0.854289 | 1.36E-61 | -1.69E-46 |
| MS.gene054810 | MS.gene29692 | 0.806835 | 6.78E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29692.t1 | MTR_5g083270 | 95.610 | 205 | 9 | 0 | 1 | 205 | 1 | 205 | 2.50e-146 | 405 |
| MS.gene29692.t1 | MTR_3g071740 | 62.946 | 224 | 62 | 5 | 1 | 204 | 1 | 223 | 5.46e-84 | 248 |
| MS.gene29692.t1 | MTR_4g014460 | 64.327 | 171 | 54 | 4 | 39 | 205 | 42 | 209 | 1.17e-71 | 216 |
| MS.gene29692.t1 | MTR_7g070050 | 68.000 | 175 | 46 | 4 | 39 | 205 | 6 | 178 | 1.98e-70 | 213 |
| MS.gene29692.t1 | MTR_5g030500 | 55.488 | 164 | 66 | 3 | 46 | 203 | 44 | 206 | 2.16e-60 | 188 |
| MS.gene29692.t1 | MTR_8g027805 | 52.353 | 170 | 73 | 4 | 41 | 205 | 11 | 177 | 7.23e-58 | 181 |
| MS.gene29692.t1 | MTR_3g090980 | 51.534 | 163 | 73 | 3 | 44 | 203 | 19 | 178 | 2.94e-57 | 179 |
| MS.gene29692.t1 | MTR_1g028380 | 50.909 | 165 | 75 | 2 | 44 | 205 | 17 | 178 | 8.67e-57 | 178 |
| MS.gene29692.t1 | MTR_1g016480 | 52.795 | 161 | 69 | 3 | 46 | 202 | 39 | 196 | 1.86e-54 | 172 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29692.t1 | AT2G40330 | 58.571 | 210 | 79 | 3 | 1 | 203 | 1 | 209 | 1.57e-81 | 242 |
| MS.gene29692.t1 | AT5G05440 | 60.396 | 202 | 74 | 3 | 1 | 200 | 1 | 198 | 7.60e-77 | 229 |
| MS.gene29692.t1 | AT2G38310 | 65.285 | 193 | 60 | 3 | 13 | 203 | 8 | 195 | 2.19e-73 | 221 |
| MS.gene29692.t1 | AT1G01360 | 52.147 | 163 | 72 | 2 | 46 | 205 | 21 | 180 | 1.79e-60 | 187 |
| MS.gene29692.t1 | AT4G27920 | 50.909 | 165 | 76 | 2 | 44 | 205 | 12 | 174 | 1.12e-57 | 180 |
| MS.gene29692.t1 | AT4G01026 | 52.201 | 159 | 70 | 3 | 50 | 205 | 27 | 182 | 3.45e-56 | 177 |
| MS.gene29692.t1 | AT5G53160 | 50.909 | 165 | 75 | 3 | 44 | 205 | 17 | 178 | 3.84e-56 | 176 |
| MS.gene29692.t1 | AT4G17870 | 50.610 | 164 | 77 | 2 | 45 | 205 | 16 | 178 | 3.80e-53 | 169 |
| MS.gene29692.t1 | AT2G26040 | 49.383 | 162 | 75 | 3 | 46 | 202 | 22 | 181 | 1.34e-50 | 162 |
| MS.gene29692.t1 | AT1G73000 | 47.619 | 168 | 77 | 3 | 46 | 202 | 37 | 204 | 9.96e-50 | 160 |
| MS.gene29692.t1 | AT5G46790 | 51.765 | 170 | 75 | 2 | 42 | 205 | 40 | 208 | 8.96e-49 | 159 |
| MS.gene29692.t1 | AT5G46790 | 51.765 | 170 | 75 | 2 | 42 | 205 | 40 | 208 | 8.96e-49 | 159 |
| MS.gene29692.t1 | AT5G45860 | 58.389 | 149 | 58 | 3 | 59 | 205 | 10 | 156 | 1.16e-46 | 151 |
| MS.gene29692.t1 | AT5G45870 | 56.954 | 151 | 62 | 3 | 56 | 205 | 7 | 155 | 2.10e-45 | 148 |
| MS.gene29692.t1 | AT4G18620 | 46.914 | 162 | 79 | 2 | 51 | 205 | 1 | 162 | 1.19e-35 | 123 |
| MS.gene29692.t1 | AT5G53160 | 46.667 | 105 | 53 | 2 | 44 | 148 | 17 | 118 | 5.38e-28 | 103 |
Find 59 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGTGGTTTGCAACCGTTTC+TGG | 0.080678 | 5.3:+67470750 | None:intergenic |
| CGTGAAGTTCGACTTGTTTC+CGG | 0.131980 | 5.3:-67470552 | MS.gene29692:CDS |
| AAACGGAGGCCTTGATGGTT+TGG | 0.349101 | 5.3:+67470685 | None:intergenic |
| GTGTTGTTGGTGGTGTTCAT+AGG | 0.363891 | 5.3:-67470461 | MS.gene29692:CDS |
| TCTAACACCGCCATGACTAA+TGG | 0.405607 | 5.3:-67470843 | MS.gene29692:CDS |
| GTTATTAGTTTCAGTGTTGT+TGG | 0.408321 | 5.3:-67470474 | MS.gene29692:CDS |
| TTCATAGGTGTAGGAATTAC+CGG | 0.411086 | 5.3:-67470446 | MS.gene29692:CDS |
| AGGCCTCCGTTTCAACTGTC+TGG | 0.430513 | 5.3:-67470674 | MS.gene29692:CDS |
| CGACACGCTTGAATTTGTTT+CGG | 0.430906 | 5.3:+67470810 | None:intergenic |
| AGTTGAAACGGAGGCCTTGA+TGG | 0.442287 | 5.3:+67470680 | None:intergenic |
| TCGGTGTAATTTGCAGTCAC+TGG | 0.459352 | 5.3:-67470304 | MS.gene29692:CDS |
| GGTGGTTTGCAACCGTTTCT+GGG | 0.462922 | 5.3:+67470751 | None:intergenic |
| AACGTAGTTGCCTCCGGTGA+TGG | 0.485651 | 5.3:-67470594 | MS.gene29692:CDS |
| AACCGTTTCTGTCCCAGAAA+CGG | 0.488981 | 5.3:-67470763 | MS.gene29692:CDS |
| AGCTGCAACGTAGTTGCCTC+CGG | 0.500297 | 5.3:-67470600 | MS.gene29692:CDS |
| GCTTGAAACGGCAGGTAGAC+CGG | 0.502003 | 5.3:+67470533 | None:intergenic |
| CAACCTCTCCATGCTTGAAA+CGG | 0.516613 | 5.3:+67470521 | None:intergenic |
| GGATCACGACGGAGTAGCAC+TGG | 0.520709 | 5.3:+67470706 | None:intergenic |
| AACCGTTTCTGGGACAGAAA+CGG | 0.521454 | 5.3:+67470761 | None:intergenic |
| CGTTTCAAGCATGGAGAGGT+TGG | 0.529991 | 5.3:-67470520 | MS.gene29692:CDS |
| TCGAACCGTTGAAATTGAAC+CGG | 0.530260 | 5.3:+67470867 | None:intergenic |
| CGTTGAAATTGAACCGGTGA+AGG | 0.532412 | 5.3:+67470873 | None:intergenic |
| ACCGACACGAATGCCATCAC+CGG | 0.534492 | 5.3:+67470581 | None:intergenic |
| AACGGACCAGACAGTTGAAA+CGG | 0.535986 | 5.3:+67470668 | None:intergenic |
| CAAAATAGAAATGCCTTCAC+CGG | 0.537348 | 5.3:-67470886 | None:intergenic |
| CTTCACCGGTTCAATTTCAA+CGG | 0.537786 | 5.3:-67470872 | MS.gene29692:CDS |
| CTCTCCATGCTTGAAACGGC+AGG | 0.544665 | 5.3:+67470525 | None:intergenic |
| ACAAAGTGTTTGTAGCCTTG+TGG | 0.546590 | 5.3:+67470627 | None:intergenic |
| TCTACCTGCCGTTTCAAGCA+TGG | 0.550915 | 5.3:-67470529 | MS.gene29692:CDS |
| CGGTCAGTGACGACGTTGCA+CGG | 0.551799 | 5.3:-67470426 | MS.gene29692:CDS |
| TTGGTGGTGTTCATAGGTGT+AGG | 0.559793 | 5.3:-67470455 | MS.gene29692:CDS |
| ATTAGTTTCAGTGTTGTTGG+TGG | 0.561597 | 5.3:-67470471 | MS.gene29692:CDS |
| CGTCGTGATCCAAACCATCA+AGG | 0.571418 | 5.3:-67470694 | MS.gene29692:CDS |
| ATACGTCGTTGATGTACCAC+AGG | 0.575028 | 5.3:-67470370 | MS.gene29692:CDS |
| CGTGCAACGTCGTCACTGAC+CGG | 0.576112 | 5.3:+67470427 | None:intergenic |
| CAACACTGAAACTAATAACA+TGG | 0.585088 | 5.3:+67470478 | None:intergenic |
| ACAGTTAATGCCATTAGTCA+TGG | 0.586782 | 5.3:+67470833 | None:intergenic |
| ACGTTGCACGGTGACGGTAA+CGG | 0.591404 | 5.3:-67470414 | MS.gene29692:CDS |
| GTGACGACGTTGCACGGTGA+CGG | 0.594931 | 5.3:-67470420 | MS.gene29692:CDS |
| AGCCTTGTGGATTGTCGAAG+CGG | 0.601019 | 5.3:+67470640 | None:intergenic |
| AACCACCACATGCACGTGGT+GGG | 0.602296 | 5.3:-67470735 | MS.gene29692:CDS |
| TCCGGTGATGGCATTCGTGT+CGG | 0.606951 | 5.3:-67470582 | MS.gene29692:CDS |
| TGTACCACAGGGTAACACTA+AGG | 0.609377 | 5.3:-67470358 | MS.gene29692:CDS |
| CGCCGCTTCGACAATCCACA+AGG | 0.611657 | 5.3:-67470642 | MS.gene29692:CDS |
| AAACCACCACATGCACGTGG+TGG | 0.622421 | 5.3:-67470736 | MS.gene29692:CDS |
| GTCCCACCACGTGCATGTGG+TGG | 0.623392 | 5.3:+67470733 | None:intergenic |
| CTGCCGTTTCAAGCATGGAG+AGG | 0.630256 | 5.3:-67470524 | MS.gene29692:CDS |
| ATTGTCGAAGCGGCGGACAA+CGG | 0.630831 | 5.3:+67470650 | None:intergenic |
| CTGCAAATTACACCGAACAA+TGG | 0.632705 | 5.3:+67470311 | None:intergenic |
| TGCAAACCACCACATGCACG+TGG | 0.642151 | 5.3:-67470739 | MS.gene29692:CDS |
| TTTGTCCCACCACGTGCATG+TGG | 0.645948 | 5.3:+67470730 | None:intergenic |
| TCTTCCTTAGTGTTACCCTG+TGG | 0.656284 | 5.3:+67470354 | None:intergenic |
| GACACGAATGCCATCACCGG+AGG | 0.657865 | 5.3:+67470584 | None:intergenic |
| CTTGTGGATTGTCGAAGCGG+CGG | 0.689521 | 5.3:+67470643 | None:intergenic |
| CTTGATGGTTTGGATCACGA+CGG | 0.697791 | 5.3:+67470695 | None:intergenic |
| GGACCAGACAGTTGAAACGG+AGG | 0.712986 | 5.3:+67470671 | None:intergenic |
| GTTAATGCCATTAGTCATGG+CGG | 0.726535 | 5.3:+67470836 | None:intergenic |
| TTGCACGGTGACGGTAACGG+AGG | 0.729282 | 5.3:-67470411 | MS.gene29692:CDS |
| TACGTCGTTGATGTACCACA+GGG | 0.754269 | 5.3:-67470369 | MS.gene29692:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CAACACTGAAACTAATAACA+TGG | + | chr5.3:67470682-67470701 | None:intergenic | 30.0% | |
| ! | GTTATTAGTTTCAGTGTTGT+TGG | - | chr5.3:67470683-67470702 | MS.gene29692:CDS | 30.0% |
| !! | GTTTTGTTGATACCATTGTT+CGG | - | chr5.3:67470834-67470853 | MS.gene29692:CDS | 30.0% |
| ACAGTTAATGCCATTAGTCA+TGG | + | chr5.3:67470327-67470346 | None:intergenic | 35.0% | |
| TTCATAGGTGTAGGAATTAC+CGG | - | chr5.3:67470711-67470730 | MS.gene29692:CDS | 35.0% | |
| ! | ATTAGTTTCAGTGTTGTTGG+TGG | - | chr5.3:67470686-67470705 | MS.gene29692:CDS | 35.0% |
| CTGCAAATTACACCGAACAA+TGG | + | chr5.3:67470849-67470868 | None:intergenic | 40.0% | |
| GTTAATGCCATTAGTCATGG+CGG | + | chr5.3:67470324-67470343 | None:intergenic | 40.0% | |
| TCGAACCGTTGAAATTGAAC+CGG | + | chr5.3:67470293-67470312 | None:intergenic | 40.0% | |
| ! | ACAAAGTGTTTGTAGCCTTG+TGG | + | chr5.3:67470533-67470552 | None:intergenic | 40.0% |
| ! | CGACACGCTTGAATTTGTTT+CGG | + | chr5.3:67470350-67470369 | None:intergenic | 40.0% |
| !! | CTTCACCGGTTCAATTTCAA+CGG | - | chr5.3:67470285-67470304 | MS.gene29692:CDS | 40.0% |
| AACCGTTTCTGTCCCAGAAA+CGG | - | chr5.3:67470394-67470413 | MS.gene29692:CDS | 45.0% | |
| AACGGACCAGACAGTTGAAA+CGG | + | chr5.3:67470492-67470511 | None:intergenic | 45.0% | |
| ATACGTCGTTGATGTACCAC+AGG | - | chr5.3:67470787-67470806 | MS.gene29692:CDS | 45.0% | |
| CAACCTCTCCATGCTTGAAA+CGG | + | chr5.3:67470639-67470658 | None:intergenic | 45.0% | |
| CGTTGAAATTGAACCGGTGA+AGG | + | chr5.3:67470287-67470306 | None:intergenic | 45.0% | |
| TACGTCGTTGATGTACCACA+GGG | - | chr5.3:67470788-67470807 | MS.gene29692:CDS | 45.0% | |
| TCTAACACCGCCATGACTAA+TGG | - | chr5.3:67470314-67470333 | MS.gene29692:CDS | 45.0% | |
| TCTTCCTTAGTGTTACCCTG+TGG | + | chr5.3:67470806-67470825 | None:intergenic | 45.0% | |
| TGTACCACAGGGTAACACTA+AGG | - | chr5.3:67470799-67470818 | MS.gene29692:CDS | 45.0% | |
| ! | CGTGAAGTTCGACTTGTTTC+CGG | - | chr5.3:67470605-67470624 | MS.gene29692:CDS | 45.0% |
| ! | CTTGATGGTTTGGATCACGA+CGG | + | chr5.3:67470465-67470484 | None:intergenic | 45.0% |
| ! | TCGGTGTAATTTGCAGTCAC+TGG | - | chr5.3:67470853-67470872 | MS.gene29692:CDS | 45.0% |
| !! | AACCGTTTCTGGGACAGAAA+CGG | + | chr5.3:67470399-67470418 | None:intergenic | 45.0% |
| !! | GTGTTGTTGGTGGTGTTCAT+AGG | - | chr5.3:67470696-67470715 | MS.gene29692:CDS | 45.0% |
| !! | TTGGTGGTGTTCATAGGTGT+AGG | - | chr5.3:67470702-67470721 | MS.gene29692:CDS | 45.0% |
| AGTTGAAACGGAGGCCTTGA+TGG | + | chr5.3:67470480-67470499 | None:intergenic | 50.0% | |
| CGTCGTGATCCAAACCATCA+AGG | - | chr5.3:67470463-67470482 | MS.gene29692:CDS | 50.0% | |
| CGTTTCAAGCATGGAGAGGT+TGG | - | chr5.3:67470637-67470656 | MS.gene29692:CDS | 50.0% | |
| ! | AAACGGAGGCCTTGATGGTT+TGG | + | chr5.3:67470475-67470494 | None:intergenic | 50.0% |
| ! | AGCCTTGTGGATTGTCGAAG+CGG | + | chr5.3:67470520-67470539 | None:intergenic | 50.0% |
| ! | GGTGGTTTGCAACCGTTTCT+GGG | + | chr5.3:67470409-67470428 | None:intergenic | 50.0% |
| ! | TCTACCTGCCGTTTCAAGCA+TGG | - | chr5.3:67470628-67470647 | MS.gene29692:CDS | 50.0% |
| ! | TGGTGGTTTGCAACCGTTTC+TGG | + | chr5.3:67470410-67470429 | None:intergenic | 50.0% |
| AAACCACCACATGCACGTGG+TGG | - | chr5.3:67470421-67470440 | MS.gene29692:CDS | 55.0% | |
| AACCACCACATGCACGTGGT+GGG | - | chr5.3:67470422-67470441 | MS.gene29692:CDS | 55.0% | |
| AACGTAGTTGCCTCCGGTGA+TGG | - | chr5.3:67470563-67470582 | MS.gene29692:CDS | 55.0% | |
| ACCGACACGAATGCCATCAC+CGG | + | chr5.3:67470579-67470598 | None:intergenic | 55.0% | |
| AGCTGCAACGTAGTTGCCTC+CGG | - | chr5.3:67470557-67470576 | MS.gene29692:CDS | 55.0% | |
| AGGCCTCCGTTTCAACTGTC+TGG | - | chr5.3:67470483-67470502 | MS.gene29692:CDS | 55.0% | |
| ATTGTCGAAGCGGCGGACAA+CGG | + | chr5.3:67470510-67470529 | None:intergenic | 55.0% | |
| CTCTCCATGCTTGAAACGGC+AGG | + | chr5.3:67470635-67470654 | None:intergenic | 55.0% | |
| GCTTGAAACGGCAGGTAGAC+CGG | + | chr5.3:67470627-67470646 | None:intergenic | 55.0% | |
| GGACCAGACAGTTGAAACGG+AGG | + | chr5.3:67470489-67470508 | None:intergenic | 55.0% | |
| TGCAAACCACCACATGCACG+TGG | - | chr5.3:67470418-67470437 | MS.gene29692:CDS | 55.0% | |
| TTTGTCCCACCACGTGCATG+TGG | + | chr5.3:67470430-67470449 | None:intergenic | 55.0% | |
| ! | ACGTTGCACGGTGACGGTAA+CGG | - | chr5.3:67470743-67470762 | MS.gene29692:CDS | 55.0% |
| ! | CTGCCGTTTCAAGCATGGAG+AGG | - | chr5.3:67470633-67470652 | MS.gene29692:CDS | 55.0% |
| ! | CTTGTGGATTGTCGAAGCGG+CGG | + | chr5.3:67470517-67470536 | None:intergenic | 55.0% |
| ! | TCCGGTGATGGCATTCGTGT+CGG | - | chr5.3:67470575-67470594 | MS.gene29692:CDS | 55.0% |
| CGCCGCTTCGACAATCCACA+AGG | - | chr5.3:67470515-67470534 | MS.gene29692:CDS | 60.0% | |
| CGGTCAGTGACGACGTTGCA+CGG | - | chr5.3:67470731-67470750 | MS.gene29692:CDS | 60.0% | |
| CGTGCAACGTCGTCACTGAC+CGG | + | chr5.3:67470733-67470752 | None:intergenic | 60.0% | |
| GACACGAATGCCATCACCGG+AGG | + | chr5.3:67470576-67470595 | None:intergenic | 60.0% | |
| GGATCACGACGGAGTAGCAC+TGG | + | chr5.3:67470454-67470473 | None:intergenic | 60.0% | |
| GTGACGACGTTGCACGGTGA+CGG | - | chr5.3:67470737-67470756 | MS.gene29692:CDS | 60.0% | |
| ! | TTGCACGGTGACGGTAACGG+AGG | - | chr5.3:67470746-67470765 | MS.gene29692:CDS | 60.0% |
| GTCCCACCACGTGCATGTGG+TGG | + | chr5.3:67470427-67470446 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 67470281 | 67470898 | 67470281 | ID=MS.gene29692 |
| chr5.3 | mRNA | 67470281 | 67470898 | 67470281 | ID=MS.gene29692.t1;Parent=MS.gene29692 |
| chr5.3 | exon | 67470281 | 67470898 | 67470281 | ID=MS.gene29692.t1.exon1;Parent=MS.gene29692.t1 |
| chr5.3 | CDS | 67470281 | 67470898 | 67470281 | ID=cds.MS.gene29692.t1;Parent=MS.gene29692.t1 |
| Gene Sequence |
| Protein sequence |
