Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30676.t1 | RHN42279.1 | 57.4 | 408 | 18 | 2 | 1 | 252 | 1193 | 1600 | 1.90E-108 | 402.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30676.t1 | Q8BV79 | 33.8 | 222 | 125 | 5 | 45 | 252 | 1507 | 1720 | 9.4e-24 | 112.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30676.t1 | A0A072TGW6 | 57.4 | 408 | 18 | 2 | 1 | 252 | 1196 | 1603 | 1.4e-108 | 402.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050442 | MS.gene30676 | 0.81078 | 9.72E-51 | -1.69E-46 |
| MS.gene05510 | MS.gene30676 | 0.806227 | 9.11E-50 | -1.69E-46 |
| MS.gene057869 | MS.gene30676 | 0.814442 | 1.54E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30676.t1 | MTR_0189s0030 | 93.301 | 209 | 13 | 1 | 45 | 252 | 1395 | 1603 | 1.78e-128 | 401 |
| MS.gene30676.t1 | MTR_0189s0030 | 83.673 | 49 | 8 | 0 | 1 | 49 | 1196 | 1244 | 2.53e-19 | 87.8 |
| MS.gene30676.t1 | MTR_3g436400 | 58.294 | 211 | 84 | 3 | 45 | 252 | 1388 | 1597 | 4.09e-68 | 228 |
| MS.gene30676.t1 | MTR_5g080800 | 86.957 | 46 | 6 | 0 | 1 | 46 | 453 | 498 | 5.79e-18 | 83.6 |
| MS.gene30676.t1 | MTR_5g080800 | 84.211 | 38 | 6 | 0 | 83 | 120 | 630 | 667 | 2.30e-13 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 103 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTTTTGTTTATTTATTTTAA+TGG | - | 42993:18203-18222 | MS.gene30676:intron | 10.0% |
| !! | AAAAAATGAAGATGAACAAA+GGG | - | 42993:17480-17499 | MS.gene30676:CDS | 20.0% |
| !! | AAATCATGTCTCATATAAAA+GGG | - | 42993:17327-17346 | MS.gene30676:intron | 20.0% |
| !!! | AAAGTTTTGATTCAACAAAA+AGG | + | 42993:17817-17836 | None:intergenic | 20.0% |
| !!! | TTTTATATGAGACATGATTT+CGG | + | 42993:17327-17346 | None:intergenic | 20.0% |
| ! | AAAAATGAAGATGAACAAAG+GGG | - | 42993:17481-17500 | MS.gene30676:CDS | 25.0% |
| ! | AAATAAAGCAATTTGACTCA+TGG | + | 42993:17615-17634 | None:intergenic | 25.0% |
| ! | AATCATGTCTCATATAAAAG+GGG | - | 42993:17328-17347 | MS.gene30676:intron | 25.0% |
| ! | ACAAAATTAATCAGACAAGA+TGG | + | 42993:18176-18195 | None:intergenic | 25.0% |
| ! | ACAGGTTTGATTCATTATAT+TGG | - | 42993:17249-17268 | MS.gene30676:CDS | 25.0% |
| ! | ACTCAGAATTAACAAATATG+AGG | - | 42993:17544-17563 | MS.gene30676:CDS | 25.0% |
| ! | ATCACTAAATCTTTCGAAAA+AGG | + | 42993:17150-17169 | None:intergenic | 25.0% |
| ! | CTCAGAATTAACAAATATGA+GGG | - | 42993:17545-17564 | MS.gene30676:CDS | 25.0% |
| ! | GAAAAAATGAAGATGAACAA+AGG | - | 42993:17479-17498 | MS.gene30676:CDS | 25.0% |
| ! | GAAATCATGTCTCATATAAA+AGG | - | 42993:17326-17345 | MS.gene30676:intron | 25.0% |
| ! | TGTATATATTGATGAAGTTC+AGG | - | 42993:17583-17602 | MS.gene30676:CDS | 25.0% |
| ! | TTTAGTGATCAATCTTCTTT+AGG | - | 42993:17161-17180 | MS.gene30676:CDS | 25.0% |
| !! | AAGGAAATTTTAGACTATGT+TGG | - | 42993:18086-18105 | MS.gene30676:intron | 25.0% |
| !! | AATGCCATTGTAACTATTTT+TGG | - | 42993:17984-18003 | MS.gene30676:intron | 25.0% |
| !!! | TAACTATTTTGAAGTGCAAA+GGG | - | 42993:18126-18145 | MS.gene30676:intron | 25.0% |
| !!! | TTAACTATTTTGAAGTGCAA+AGG | - | 42993:18125-18144 | MS.gene30676:intron | 25.0% |
| AAAACGATAAAGAAGCTCAA+TGG | + | 42993:17877-17896 | None:intergenic | 30.0% | |
| AAACAACTATATGTGGCTAT+AGG | - | 42993:18405-18424 | MS.gene30676:CDS | 30.0% | |
| AATCTAGCATCTTGGTAAAT+TGG | + | 42993:17289-17308 | None:intergenic | 30.0% | |
| AGAACACAAAAAGTTGTGTT+TGG | + | 42993:18379-18398 | None:intergenic | 30.0% | |
| ATCATGTCTCATATAAAAGG+GGG | - | 42993:17329-17348 | MS.gene30676:intron | 30.0% | |
| ATCTAGCATCTTGGTAAATT+GGG | + | 42993:17288-17307 | None:intergenic | 30.0% | |
| GTTGCCAAAAATAGTTACAA+TGG | + | 42993:17991-18010 | None:intergenic | 30.0% | |
| TATGAATGAACAAGATATGC+TGG | - | 42993:18314-18333 | MS.gene30676:CDS | 30.0% | |
| TCAAAACATCAATGGAATGA+GGG | + | 42993:17902-17921 | None:intergenic | 30.0% | |
| TGAGCTTAAACAACTATATG+TGG | - | 42993:18398-18417 | MS.gene30676:CDS | 30.0% | |
| TTCAAAACATCAATGGAATG+AGG | + | 42993:17903-17922 | None:intergenic | 30.0% | |
| ! | ACCTTTGAAAAATCAATGGA+GGG | - | 42993:18278-18297 | MS.gene30676:intron | 30.0% |
| ! | CTTCACCTTTGAAAAATCAA+TGG | - | 42993:18274-18293 | MS.gene30676:intron | 30.0% |
| ! | TGTTTGGAATCATTGAAACT+GGG | + | 42993:18363-18382 | None:intergenic | 30.0% |
| !!! | TTGCTTTTGAGACTTTTACA+AGG | - | 42993:17207-17226 | MS.gene30676:CDS | 30.0% |
| !!! | TTTTGAGACTTTTACAAGGA+AGG | - | 42993:17211-17230 | MS.gene30676:CDS | 30.0% |
| AAAATCAATTCCCCTTGCAA+TGG | + | 42993:17697-17716 | None:intergenic | 35.0% | |
| ACAAGAGAGATTTGATATCC+TGG | + | 42993:17725-17744 | None:intergenic | 35.0% | |
| ACTAGGAAAGATAATCCTGA+CGG | + | 42993:17391-17410 | None:intergenic | 35.0% | |
| ATCCTTAAGCAAGCAGAAAA+GGG | - | 42993:17427-17446 | MS.gene30676:CDS | 35.0% | |
| ATTGAAACTGGGAAAAGACT+TGG | + | 42993:18352-18371 | None:intergenic | 35.0% | |
| CTCAGGTTTCAAAACATCAA+TGG | + | 42993:17910-17929 | None:intergenic | 35.0% | |
| GTAAATTGGGAGTTGAAATG+TGG | + | 42993:17275-17294 | None:intergenic | 35.0% | |
| ! | AAGAGAAGTTCTGATAACCA+CGG | - | 42993:17765-17784 | MS.gene30676:CDS | 35.0% |
| ! | AAGAGGGTTTCGTATTTTGT+GGG | - | 42993:17658-17677 | MS.gene30676:CDS | 35.0% |
| ! | CACCTTTGAAAAATCAATGG+AGG | - | 42993:18277-18296 | MS.gene30676:intron | 35.0% |
| ! | CTTTCACTTTATCTTTTCCG+TGG | + | 42993:17785-17804 | None:intergenic | 35.0% |
| ! | GAAACCCTCTTCTACATTTT+CGG | + | 42993:17649-17668 | None:intergenic | 35.0% |
| ! | GTGTTTGGAATCATTGAAAC+TGG | + | 42993:18364-18383 | None:intergenic | 35.0% |
| !! | TTGCAAGGGGAATTGATTTT+AGG | - | 42993:17697-17716 | MS.gene30676:CDS | 35.0% |
| !!! | ACCCTCCATTGATTTTTCAA+AGG | + | 42993:18282-18301 | None:intergenic | 35.0% |
| !!! | GGTCATTTGTGTTATTTTGC+AGG | - | 42993:18224-18243 | MS.gene30676:intron | 35.0% |
| !!! | GTAGTGCTGTACAACTTTTT+TGG | - | 42993:18249-18268 | MS.gene30676:intron | 35.0% |
| AAGCAACAGATCTTACACCA+AGG | + | 42993:17193-17212 | None:intergenic | 40.0% | |
| CAAAACTTTCGAACTCATGC+TGG | - | 42993:17828-17847 | MS.gene30676:intron | 40.0% | |
| CATCCTTAAGCAAGCAGAAA+AGG | - | 42993:17426-17445 | MS.gene30676:CDS | 40.0% | |
| CATGACAGTGGGAAAATTGT+TGG | - | 42993:18017-18036 | MS.gene30676:intron | 40.0% | |
| CCTGAGACCAGTTTGATATA+CGG | - | 42993:17924-17943 | MS.gene30676:intron | 40.0% | |
| CTATGTTGGAAAACAAGCTC+TGG | - | 42993:18100-18119 | MS.gene30676:intron | 40.0% | |
| CTGAGACCAGTTTGATATAC+GGG | - | 42993:17925-17944 | MS.gene30676:intron | 40.0% | |
| CTTCCACATTCAAGAACAAC+TGG | + | 42993:17957-17976 | None:intergenic | 40.0% | |
| GGAAAAGACTTGGATTCTGT+AGG | + | 42993:18342-18361 | None:intergenic | 40.0% | |
| TAATTCTGAGTCGACGATGA+AGG | + | 42993:17535-17554 | None:intergenic | 40.0% | |
| TCTGGATGAATCTAGCATCT+TGG | + | 42993:17297-17316 | None:intergenic | 40.0% | |
| TCTTCTTTAGGTGAGAACCT+TGG | - | 42993:17173-17192 | MS.gene30676:CDS | 40.0% | |
| TGAGACCAGTTTGATATACG+GGG | - | 42993:17926-17945 | MS.gene30676:intron | 40.0% | |
| TGTGCCGAAAATGTAGAAGA+GGG | - | 42993:17642-17661 | MS.gene30676:CDS | 40.0% | |
| ! | AGAGGGTTTCGTATTTTGTG+GGG | - | 42993:17659-17678 | MS.gene30676:CDS | 40.0% |
| ! | CTTTTCTGCTTGCTTAAGGA+TGG | + | 42993:17428-17447 | None:intergenic | 40.0% |
| ! | GAAGAGGGTTTCGTATTTTG+TGG | - | 42993:17657-17676 | MS.gene30676:CDS | 40.0% |
| ! | TGAGACTTTTACAAGGAAGG+AGG | - | 42993:17214-17233 | MS.gene30676:CDS | 40.0% |
| ! | TGGAGCAAAGCAAGTGATTT+TGG | - | 42993:18043-18062 | MS.gene30676:intron | 40.0% |
| !! | AGTGGGAAAATTGTTGGCTT+TGG | - | 42993:18023-18042 | MS.gene30676:intron | 40.0% |
| !!! | GGGAATTGATTTTAGGTTCC+AGG | - | 42993:17704-17723 | MS.gene30676:CDS | 40.0% |
| !!! | GTAACTATTTTTGGCAACCC+TGG | - | 42993:17993-18012 | MS.gene30676:intron | 40.0% |
| AAAAGGGGGAATGCAATCTC+TGG | - | 42993:17343-17362 | MS.gene30676:intron | 45.0% | |
| AAAGAAGCTCAATGGTGCTC+TGG | + | 42993:17869-17888 | None:intergenic | 45.0% | |
| AAGAAGCTCAATGGTGCTCT+GGG | + | 42993:17868-17887 | None:intergenic | 45.0% | |
| AGCTTCCCCGTATATCAAAC+TGG | + | 42993:17934-17953 | None:intergenic | 45.0% | |
| AGGAGGAAGTGACTTATGAC+AGG | - | 42993:17231-17250 | MS.gene30676:CDS | 45.0% | |
| ATACTGCACAGACCATTGCA+AGG | - | 42993:17682-17701 | MS.gene30676:CDS | 45.0% | |
| ATGATGCTTGATGGGACTGT+GGG | - | 42993:17119-17138 | MS.gene30676:CDS | 45.0% | |
| CATGATTTCGGTGAACACTC+TGG | + | 42993:17315-17334 | None:intergenic | 45.0% | |
| CCTTCCGAGCGTAATCATAA+CGG | + | 42993:18070-18089 | None:intergenic | 45.0% | |
| GCTCCAGTTGTTCTTGAATG+TGG | - | 42993:17951-17970 | MS.gene30676:intron | 45.0% | |
| GTGTGCCGAAAATGTAGAAG+AGG | - | 42993:17641-17660 | MS.gene30676:CDS | 45.0% | |
| TACTGCACAGACCATTGCAA+GGG | - | 42993:17683-17702 | MS.gene30676:CDS | 45.0% | |
| TGGTCCGTTATGATTACGCT+CGG | - | 42993:18063-18082 | MS.gene30676:intron | 45.0% | |
| ! | ATTTTCCCACTGTCATGTCC+AGG | + | 42993:18014-18033 | None:intergenic | 45.0% |
| ! | CCGTATATCAAACTGGTCTC+AGG | + | 42993:17927-17946 | None:intergenic | 45.0% |
| ! | CTCCCTTTTCTGCTTGCTTA+AGG | + | 42993:17432-17451 | None:intergenic | 45.0% |
| ! | TGGCGCTAGATTTTCAGACT+AGG | + | 42993:17408-17427 | None:intergenic | 45.0% |
| ! | TTTTCCCACTGTCATGTCCA+GGG | + | 42993:18013-18032 | None:intergenic | 45.0% |
| ACTGCACAGACCATTGCAAG+GGG | - | 42993:17684-17703 | MS.gene30676:CDS | 50.0% | |
| CCGTTATGATTACGCTCGGA+AGG | - | 42993:18067-18086 | MS.gene30676:intron | 50.0% | |
| GGAATGCAATCTCTGGATCC+TGG | - | 42993:17350-17369 | MS.gene30676:intron | 50.0% | |
| GTGCAAAGGGCTTGAGTTTC+AGG | - | 42993:18139-18158 | MS.gene30676:intron | 50.0% | |
| TGAAGAGAAGTTGAGCCGTC+AGG | - | 42993:17373-17392 | MS.gene30676:intron | 50.0% | |
| TGATGCTTGATGGGACTGTG+GGG | - | 42993:17120-17139 | MS.gene30676:CDS | 50.0% | |
| CGGCTCAACTTCTCTTCACC+AGG | + | 42993:17371-17390 | None:intergenic | 55.0% | |
| GCAACCCTGGACATGACAGT+GGG | - | 42993:18006-18025 | MS.gene30676:intron | 55.0% | |
| GGCAACCCTGGACATGACAG+TGG | - | 42993:18005-18024 | MS.gene30676:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 42993 | gene | 17119 | 18437 | 17119 | ID=MS.gene30676 |
| 42993 | mRNA | 17119 | 18437 | 17119 | ID=MS.gene30676.t1;Parent=MS.gene30676 |
| 42993 | exon | 18304 | 18437 | 18304 | ID=MS.gene30676.t1.exon1;Parent=MS.gene30676.t1 |
| 42993 | CDS | 18304 | 18437 | 18304 | ID=cds.MS.gene30676.t1;Parent=MS.gene30676.t1 |
| 42993 | exon | 17396 | 17837 | 17396 | ID=MS.gene30676.t1.exon2;Parent=MS.gene30676.t1 |
| 42993 | CDS | 17396 | 17837 | 17396 | ID=cds.MS.gene30676.t1;Parent=MS.gene30676.t1 |
| 42993 | exon | 17119 | 17310 | 17119 | ID=MS.gene30676.t1.exon3;Parent=MS.gene30676.t1 |
| 42993 | CDS | 17119 | 17310 | 17119 | ID=cds.MS.gene30676.t1;Parent=MS.gene30676.t1 |
| Gene Sequence |
| Protein sequence |
