AlfalfaGEDB | MS.gene31088

M. sativa cultivar XinJiangDaYe / MS.gene31088

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31088.t1	XP_024631646.1	98.2	170	3	0	1	170	1	170	5.90E-90	340.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31088.t1	Q8W031	49.3	152	54	6	35	170	53	197	6.7e-26	118.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31088.t1	A0A396JHF9	98.2	170	3	0	1	170	1	170	4.2e-90	340.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050444	MS.gene31088	-0.81432	1.64E-51	-1.69E-46
MS.gene052050	MS.gene31088	0.99854	3.63E-268	-1.69E-46
MS.gene053776	MS.gene31088	0.813997	1.93E-51	-1.69E-46
MS.gene056678	MS.gene31088	0.804971	1.67E-49	-1.69E-46
MS.gene057246	MS.gene31088	-0.806132	9.54E-50	-1.69E-46
MS.gene058158	MS.gene31088	0.840756	7.18E-58	-1.69E-46
MS.gene058723	MS.gene31088	-0.804388	2.21E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31088.t1	MTR_1g093095	75.000	36	9	0	135	170	99	134	4.27e-14	68.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31088.t1	AT1G51220	44.505	182	73	8	6	170	27	197	7.98e-35	124
MS.gene31088.t1	AT3G20880	43.478	161	62	6	36	170	118	275	2.49e-28	108
MS.gene31088.t1	AT3G57670	38.596	171	60	3	44	170	67	236	9.88e-26	101
MS.gene31088.t1	AT1G34790	46.341	82	43	1	90	170	83	164	8.48e-19	81.6
MS.gene31088.t1	AT1G13290	45.783	83	31	2	88	170	52	120	1.19e-17	78.6
MS.gene31088.t1	AT1G08290	48.276	58	24	1	113	170	148	199	2.57e-13	67.0

Find 39 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCAATGACTTCAATGGTTAT+TGG	0.191789	2.4:-5904783	MS.gene31088:CDS
TATAGAATGGAGGGTTATTT+AGG	0.265203	2.4:+5904755	None:intergenic
GTTACTGTGGCTTTGCATCT+AGG	0.282488	2.4:-5904538	MS.gene31088:CDS
AAAGATATGTTGTTACTATA+AGG	0.326372	2.4:+5904727	None:intergenic
CATGTTGTTGTATCTATTGA+AGG	0.358064	2.4:+5904330	None:intergenic
CATCATTTCCATGGAACTAA+AGG	0.398872	2.4:+5904615	None:intergenic
CCAATAACCATTGAAGTCAT+TGG	0.409520	2.4:+5904783	None:intergenic
AGAATCTTCCTTTAGTTCCA+TGG	0.448392	2.4:-5904623	MS.gene31088:CDS
CTTCATATTCATCATTTCCA+TGG	0.449667	2.4:+5904606	None:intergenic
TTACTGTGGCTTTGCATCTA+GGG	0.458041	2.4:-5904537	MS.gene31088:CDS
GTCCACCAAATAAAATTAGC+AGG	0.460803	2.4:-5904429	MS.gene31088:CDS
ATTGAAGTCATTGGAGAAGT+AGG	0.466777	2.4:+5904792	None:intergenic
ACCCCTGCTCAGATTCTAAT+CGG	0.468227	2.4:-5904388	MS.gene31088:CDS
TCTCTTGGAGGTGGAGATGA+AGG	0.479256	2.4:+5904694	None:intergenic
AAAGGAAGAGCTTCTCTTGG+AGG	0.486389	2.4:+5904682	None:intergenic
AAATCAGATGAACTTATACT+TGG	0.487140	2.4:+5904511	None:intergenic
AGCAAAGGAAGAGCTTCTCT+TGG	0.490887	2.4:+5904679	None:intergenic
TACTTCTCCAATGACTTCAA+TGG	0.492298	2.4:-5904790	MS.gene31088:CDS
TTGAAGGTCTTGAAACAAAG+AGG	0.499110	2.4:+5904346	None:intergenic
AAATTAGCAGGGGTCAGTAT+TGG	0.506191	2.4:-5904417	MS.gene31088:CDS
TAAGGAAAATTATAGAATGG+AGG	0.508828	2.4:+5904745	None:intergenic
GGACCGATTAGAATCTGAGC+AGG	0.523985	2.4:+5904385	None:intergenic
CATGGAAATGATGAATATGA+AGG	0.524476	2.4:-5904605	MS.gene31088:CDS
GGAAGAGCTTCTCTTGGAGG+TGG	0.534319	2.4:+5904685	None:intergenic
CTATAAGGAAAATTATAGAA+TGG	0.547263	2.4:+5904742	None:intergenic
ATTAGAATCTGAGCAGGGGT+AGG	0.550899	2.4:+5904391	None:intergenic
AAGGAAAATTATAGAATGGA+GGG	0.551334	2.4:+5904746	None:intergenic
TTGAAGTCATTGGAGAAGTA+GGG	0.551474	2.4:+5904793	None:intergenic
AGAGGGCATGAAAACTGTGT+TGG	0.560971	2.4:+5904364	None:intergenic
TTAGAATCTGAGCAGGGGTA+GGG	0.569587	2.4:+5904392	None:intergenic
TCCACCAAATAAAATTAGCA+GGG	0.584942	2.4:-5904428	MS.gene31088:CDS
TGAAGGTCTTGAAACAAAGA+GGG	0.613383	2.4:+5904347	None:intergenic
GTAGGGCTTAGGTTAAGCAA+AGG	0.619991	2.4:+5904664	None:intergenic
GACCGATTAGAATCTGAGCA+GGG	0.623713	2.4:+5904386	None:intergenic
AGATGATGATGCCGTTACTG+TGG	0.634808	2.4:-5904551	MS.gene31088:CDS
CAATAGATACAACAACATGC+AGG	0.643534	2.4:-5904326	MS.gene31088:CDS
TAGATGCAAAGCCACAGTAA+CGG	0.646015	2.4:+5904540	None:intergenic
CCACCAAATAAAATTAGCAG+GGG	0.685279	2.4:-5904427	MS.gene31088:CDS
ACCGATTAGAATCTGAGCAG+GGG	0.690964	2.4:+5904387	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAGATATGTTGTTACTATA+AGG	+	chr2.4:5904396-5904415	None:intergenic	20.0%
!!	CTATAAGGAAAATTATAGAA+TGG	+	chr2.4:5904381-5904400	None:intergenic	20.0%
!	AAATCAGATGAACTTATACT+TGG	+	chr2.4:5904612-5904631	None:intergenic	25.0%
!	AAGGAAAATTATAGAATGGA+GGG	+	chr2.4:5904377-5904396	None:intergenic	25.0%
!	TAAGGAAAATTATAGAATGG+AGG	+	chr2.4:5904378-5904397	None:intergenic	25.0%
	CATGGAAATGATGAATATGA+AGG	-	chr2.4:5904515-5904534	MS.gene31088:CDS	30.0%
	CATGTTGTTGTATCTATTGA+AGG	+	chr2.4:5904793-5904812	None:intergenic	30.0%
	CTTCATATTCATCATTTCCA+TGG	+	chr2.4:5904517-5904536	None:intergenic	30.0%
	TATAGAATGGAGGGTTATTT+AGG	+	chr2.4:5904368-5904387	None:intergenic	30.0%
	TCCACCAAATAAAATTAGCA+GGG	-	chr2.4:5904692-5904711	MS.gene31088:CDS	30.0%
!	AGAAGATCTTCATGTTTTGT+AGG	+	chr2.4:5904477-5904496	None:intergenic	30.0%
!	GAAGATCTTCATGTTTTGTA+GGG	+	chr2.4:5904476-5904495	None:intergenic	30.0%
	ATTGAAGTCATTGGAGAAGT+AGG	+	chr2.4:5904331-5904350	None:intergenic	35.0%
	CAATAGATACAACAACATGC+AGG	-	chr2.4:5904794-5904813	MS.gene31088:CDS	35.0%
	CATCATTTCCATGGAACTAA+AGG	+	chr2.4:5904508-5904527	None:intergenic	35.0%
	CCAATAACCATTGAAGTCAT+TGG	+	chr2.4:5904340-5904359	None:intergenic	35.0%
	CCAATGACTTCAATGGTTAT+TGG	-	chr2.4:5904337-5904356	MS.gene31088:CDS	35.0%
	CCACCAAATAAAATTAGCAG+GGG	-	chr2.4:5904693-5904712	MS.gene31088:CDS	35.0%
	GTCCACCAAATAAAATTAGC+AGG	-	chr2.4:5904691-5904710	MS.gene31088:CDS	35.0%
	TACTTCTCCAATGACTTCAA+TGG	-	chr2.4:5904330-5904349	MS.gene31088:CDS	35.0%
	TGAAGGTCTTGAAACAAAGA+GGG	+	chr2.4:5904776-5904795	None:intergenic	35.0%
	TTGAAGGTCTTGAAACAAAG+AGG	+	chr2.4:5904777-5904796	None:intergenic	35.0%
	TTGAAGTCATTGGAGAAGTA+GGG	+	chr2.4:5904330-5904349	None:intergenic	35.0%
!	AGAATCTTCCTTTAGTTCCA+TGG	-	chr2.4:5904497-5904516	MS.gene31088:CDS	35.0%
!	TGACCCCTGCTAATTTTATT+TGG	+	chr2.4:5904699-5904718	None:intergenic	35.0%
	AAATTAGCAGGGGTCAGTAT+TGG	-	chr2.4:5904703-5904722	MS.gene31088:CDS	40.0%
	TAGATGCAAAGCCACAGTAA+CGG	+	chr2.4:5904583-5904602	None:intergenic	40.0%
!	CTTCATGTTTTGTAGGGCTT+AGG	+	chr2.4:5904470-5904489	None:intergenic	40.0%
!	TTACTGTGGCTTTGCATCTA+GGG	-	chr2.4:5904583-5904602	MS.gene31088:CDS	40.0%
!!	CCCCTGCTAATTTTATTTGG+TGG	+	chr2.4:5904696-5904715	None:intergenic	40.0%
	AAAGGAAGAGCTTCTCTTGG+AGG	+	chr2.4:5904441-5904460	None:intergenic	45.0%
	ACCCCTGCTCAGATTCTAAT+CGG	-	chr2.4:5904732-5904751	MS.gene31088:CDS	45.0%
	ACCGATTAGAATCTGAGCAG+GGG	+	chr2.4:5904736-5904755	None:intergenic	45.0%
	AGCAAAGGAAGAGCTTCTCT+TGG	+	chr2.4:5904444-5904463	None:intergenic	45.0%
	ATTAGAATCTGAGCAGGGGT+AGG	+	chr2.4:5904732-5904751	None:intergenic	45.0%
	GACCGATTAGAATCTGAGCA+GGG	+	chr2.4:5904737-5904756	None:intergenic	45.0%
	GTAGGGCTTAGGTTAAGCAA+AGG	+	chr2.4:5904459-5904478	None:intergenic	45.0%
	TTAGAATCTGAGCAGGGGTA+GGG	+	chr2.4:5904731-5904750	None:intergenic	45.0%
!	AGAGGGCATGAAAACTGTGT+TGG	+	chr2.4:5904759-5904778	None:intergenic	45.0%
!	AGATGATGATGCCGTTACTG+TGG	-	chr2.4:5904569-5904588	MS.gene31088:CDS	45.0%
!	GTTACTGTGGCTTTGCATCT+AGG	-	chr2.4:5904582-5904601	MS.gene31088:CDS	45.0%
	GGACCGATTAGAATCTGAGC+AGG	+	chr2.4:5904738-5904757	None:intergenic	50.0%
	TCTCTTGGAGGTGGAGATGA+AGG	+	chr2.4:5904429-5904448	None:intergenic	50.0%
	GGAAGAGCTTCTCTTGGAGG+TGG	+	chr2.4:5904438-5904457	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	5904315	5904827	5904315	ID=MS.gene31088
chr2.4	mRNA	5904315	5904827	5904315	ID=MS.gene31088.t1;Parent=MS.gene31088
chr2.4	exon	5904315	5904827	5904315	ID=MS.gene31088.t1.exon1;Parent=MS.gene31088.t1
chr2.4	CDS	5904315	5904827	5904315	ID=cds.MS.gene31088.t1;Parent=MS.gene31088.t1

Gene Sequence

>MS.gene31088

ATGAGTACTCATCCCTACTTCTCCAATGACTTCAATGGTTATTGGTTTGACCTAAATAACCCTCCATTCTATAATTTTCCTTATAGTAACAACATATCTTTTGCATCTTCTCCTTCATCTCCACCTCCAAGAGAAGCTCTTCCTTTGCTTAACCTAAGCCCTACAAAACATGAAGATCTTCTAGAATCTTCCTTTAGTTCCATGGAAATGATGAATATGAAGGAGAAAGAACTAAGCATATCATCAAATTCTTTAGATGATGATGCCGTTACTGTGGCTTTGCATCTAGGGCTTCCAAGTATAAGTTCATCTGATTTAGCTTCTTCAAATATTTACAAAGATGATGAAAAAGTAGTGACTGTTGATTCTTCTGAGTGTCCACCAAATAAAATTAGCAGGGGTCAGTATTGGATCCCTACCCCTGCTCAGATTCTAATCGGTCCAACACAGTTTTCATGCCCTCTTTGTTTCAAGACCTTCAATAGATACAACAACATGCAGGTATATATTTAA

Protein sequence

>MS.gene31088.t1

MSTHPYFSNDFNGYWFDLNNPPFYNFPYSNNISFASSPSSPPPREALPLLNLSPTKHEDLLESSFSSMEMMNMKEKELSISSNSLDDDAVTVALHLGLPSISSSDLASSNIYKDDEKVVTVDSSECPPNKISRGQYWIPTPAQILIGPTQFSCPLCFKTFNRYNNMQVYI