AlfalfaGEDB | MS.gene052050

M. sativa cultivar XinJiangDaYe / MS.gene052050

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052050.t1	XP_024631646.1	98.2	170	3	0	1	170	1	170	5.90E-90	340.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052050.t1	Q8W031	49.3	152	54	6	35	170	53	197	6.7e-26	118.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052050.t1	A0A396JHF9	98.2	170	3	0	1	170	1	170	4.2e-90	340.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050444	MS.gene052050	-0.8142	1.74E-51	-1.69E-46
MS.gene052050	MS.gene053776	0.811673	6.22E-51	-1.69E-46
MS.gene052050	MS.gene056678	0.805557	1.26E-49	-1.69E-46
MS.gene052050	MS.gene057246	-0.810374	1.19E-50	-1.69E-46
MS.gene052050	MS.gene058158	0.841686	4.09E-58	-1.69E-46
MS.gene052050	MS.gene058723	-0.802493	5.47E-49	-1.69E-46
MS.gene052050	MS.gene062332	-0.803372	3.60E-49	-1.69E-46
MS.gene052050	MS.gene065680	-0.816175	6.33E-52	-1.69E-46
MS.gene052050	MS.gene069871	0.820446	6.83E-53	-1.69E-46
MS.gene052050	MS.gene071307	-0.801135	1.04E-48	-1.69E-46
MS.gene052050	MS.gene071308	-0.800546	1.37E-48	-1.69E-46
MS.gene052050	MS.gene07301	-0.814581	1.43E-51	-1.69E-46
MS.gene052050	MS.gene07438	-0.802631	5.12E-49	-1.69E-46
MS.gene052050	MS.gene31088	0.99854	3.63E-268	-1.69E-46
MS.gene052050	MS.gene34093	0.856131	3.96E-62	-1.69E-46
MS.gene052050	MS.gene38127	-0.806404	8.36E-50	-1.69E-46
MS.gene052050	MS.gene40278	0.813759	2.18E-51	-1.69E-46
MS.gene052050	MS.gene40724	0.807693	4.46E-50	-1.69E-46
MS.gene052050	MS.gene45036	0.806932	6.47E-50	-1.69E-46
MS.gene052050	MS.gene45370	-0.800002	1.77E-48	-1.69E-46
MS.gene052050	MS.gene45555	0.911293	7.16E-83	-1.69E-46
MS.gene052050	MS.gene48360	0.80626	8.97E-50	-1.69E-46
MS.gene052050	MS.gene68120	-0.808966	2.39E-50	-1.69E-46
MS.gene052050	MS.gene68721	-0.80458	2.02E-49	-1.69E-46
MS.gene052050	MS.gene68946	0.800593	1.34E-48	-1.69E-46
MS.gene052050	MS.gene71692	-0.811722	6.07E-51	-1.69E-46
MS.gene052050	MS.gene73007	-0.809478	1.86E-50	-1.69E-46
MS.gene052050	MS.gene76160	0.813431	2.57E-51	-1.69E-46
MS.gene052050	MS.gene76536	0.808118	3.62E-50	-1.69E-46
MS.gene052050	MS.gene78380	0.988412	6.42E-174	-1.69E-46
MS.gene052050	MS.gene85152	0.808089	3.68E-50	-1.69E-46
MS.gene052050	MS.gene86099	-0.801008	1.10E-48	-1.69E-46
MS.gene052050	MS.gene92996	0.803146	4.01E-49	-1.69E-46
MS.gene052050	MS.gene94547	-0.820066	8.35E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052050.t1	MTR_1g093095	75.000	36	9	0	135	170	99	134	4.27e-14	68.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052050.t1	AT1G51220	44.505	182	73	8	6	170	27	197	7.98e-35	124
MS.gene052050.t1	AT3G20880	43.478	161	62	6	36	170	118	275	2.49e-28	108
MS.gene052050.t1	AT3G57670	38.596	171	60	3	44	170	67	236	9.88e-26	101
MS.gene052050.t1	AT1G34790	46.341	82	43	1	90	170	83	164	8.48e-19	81.6
MS.gene052050.t1	AT1G13290	45.783	83	31	2	88	170	52	120	1.19e-17	78.6
MS.gene052050.t1	AT1G08290	48.276	58	24	1	113	170	148	199	2.57e-13	67.0

Find 39 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCAATGACTTCAATGGTTAT+TGG	0.191789	2.2:-4544728	MS.gene052050:CDS
TATAGAATGGAGGGTTATTT+AGG	0.265203	2.2:+4544700	None:intergenic
GTTACTGTGGCTTTGCATCT+AGG	0.282488	2.2:-4544483	MS.gene052050:CDS
AAAGATATGTTGTTACTATA+AGG	0.326372	2.2:+4544672	None:intergenic
CATGTTGTTGTATCTATTGA+AGG	0.358064	2.2:+4544275	None:intergenic
CATCATTTCCATGGAACTAA+AGG	0.398872	2.2:+4544560	None:intergenic
CCAATAACCATTGAAGTCAT+TGG	0.409520	2.2:+4544728	None:intergenic
AGAATCTTCCTTTAGTTCCA+TGG	0.448392	2.2:-4544568	MS.gene052050:CDS
CTTCATATTCATCATTTCCA+TGG	0.449667	2.2:+4544551	None:intergenic
TTACTGTGGCTTTGCATCTA+GGG	0.458041	2.2:-4544482	MS.gene052050:CDS
GTCCACCAAATAAAATTAGC+AGG	0.460803	2.2:-4544374	MS.gene052050:CDS
ATTGAAGTCATTGGAGAAGT+AGG	0.466777	2.2:+4544737	None:intergenic
ACCCCTGCTCAGATTCTAAT+CGG	0.468227	2.2:-4544333	MS.gene052050:CDS
TCTCTTGGAGGTGGAGATGA+AGG	0.479256	2.2:+4544639	None:intergenic
AAAGGAAGAGCTTCTCTTGG+AGG	0.486389	2.2:+4544627	None:intergenic
AAATCAGATGAACTTATACT+TGG	0.487140	2.2:+4544456	None:intergenic
AGCAAAGGAAGAGCTTCTCT+TGG	0.490887	2.2:+4544624	None:intergenic
TACTTCTCCAATGACTTCAA+TGG	0.492298	2.2:-4544735	MS.gene052050:CDS
TTGAAGGTCTTGAAACAAAG+AGG	0.499110	2.2:+4544291	None:intergenic
AAATTAGCAGGGGTCAGTAT+TGG	0.506191	2.2:-4544362	MS.gene052050:CDS
TAAGGAAAATTATAGAATGG+AGG	0.508828	2.2:+4544690	None:intergenic
GGACCGATTAGAATCTGAGC+AGG	0.523985	2.2:+4544330	None:intergenic
CATGGAAATGATGAATATGA+AGG	0.524476	2.2:-4544550	MS.gene052050:CDS
GGAAGAGCTTCTCTTGGAGG+TGG	0.534319	2.2:+4544630	None:intergenic
CTATAAGGAAAATTATAGAA+TGG	0.547263	2.2:+4544687	None:intergenic
ATTAGAATCTGAGCAGGGGT+AGG	0.550899	2.2:+4544336	None:intergenic
AAGGAAAATTATAGAATGGA+GGG	0.551334	2.2:+4544691	None:intergenic
TTGAAGTCATTGGAGAAGTA+GGG	0.551474	2.2:+4544738	None:intergenic
AGAGGGCATGAAAACTGTGT+TGG	0.560971	2.2:+4544309	None:intergenic
TTAGAATCTGAGCAGGGGTA+GGG	0.569587	2.2:+4544337	None:intergenic
TCCACCAAATAAAATTAGCA+GGG	0.584942	2.2:-4544373	MS.gene052050:CDS
TGAAGGTCTTGAAACAAAGA+GGG	0.613383	2.2:+4544292	None:intergenic
GTAGGGCTTAGGTTAAGCAA+AGG	0.619991	2.2:+4544609	None:intergenic
GACCGATTAGAATCTGAGCA+GGG	0.623713	2.2:+4544331	None:intergenic
AGATGATGATGCCGTTACTG+TGG	0.634808	2.2:-4544496	MS.gene052050:CDS
CAATAGATACAACAACATGC+AGG	0.643534	2.2:-4544271	MS.gene052050:CDS
TAGATGCAAAGCCACAGTAA+CGG	0.646015	2.2:+4544485	None:intergenic
CCACCAAATAAAATTAGCAG+GGG	0.685279	2.2:-4544372	MS.gene052050:CDS
ACCGATTAGAATCTGAGCAG+GGG	0.690964	2.2:+4544332	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAGATATGTTGTTACTATA+AGG	+	chr2.2:4544341-4544360	None:intergenic	20.0%
!!	CTATAAGGAAAATTATAGAA+TGG	+	chr2.2:4544326-4544345	None:intergenic	20.0%
!	AAATCAGATGAACTTATACT+TGG	+	chr2.2:4544557-4544576	None:intergenic	25.0%
!	AAGGAAAATTATAGAATGGA+GGG	+	chr2.2:4544322-4544341	None:intergenic	25.0%
!	TAAGGAAAATTATAGAATGG+AGG	+	chr2.2:4544323-4544342	None:intergenic	25.0%
	CATGGAAATGATGAATATGA+AGG	-	chr2.2:4544460-4544479	MS.gene052050:CDS	30.0%
	CATGTTGTTGTATCTATTGA+AGG	+	chr2.2:4544738-4544757	None:intergenic	30.0%
	CTTCATATTCATCATTTCCA+TGG	+	chr2.2:4544462-4544481	None:intergenic	30.0%
	TATAGAATGGAGGGTTATTT+AGG	+	chr2.2:4544313-4544332	None:intergenic	30.0%
	TCCACCAAATAAAATTAGCA+GGG	-	chr2.2:4544637-4544656	MS.gene052050:CDS	30.0%
!	AGAAGATCTTCATGTTTTGT+AGG	+	chr2.2:4544422-4544441	None:intergenic	30.0%
!	GAAGATCTTCATGTTTTGTA+GGG	+	chr2.2:4544421-4544440	None:intergenic	30.0%
	ATTGAAGTCATTGGAGAAGT+AGG	+	chr2.2:4544276-4544295	None:intergenic	35.0%
	CAATAGATACAACAACATGC+AGG	-	chr2.2:4544739-4544758	MS.gene052050:CDS	35.0%
	CATCATTTCCATGGAACTAA+AGG	+	chr2.2:4544453-4544472	None:intergenic	35.0%
	CCAATAACCATTGAAGTCAT+TGG	+	chr2.2:4544285-4544304	None:intergenic	35.0%
	CCAATGACTTCAATGGTTAT+TGG	-	chr2.2:4544282-4544301	MS.gene052050:CDS	35.0%
	CCACCAAATAAAATTAGCAG+GGG	-	chr2.2:4544638-4544657	MS.gene052050:CDS	35.0%
	GTCCACCAAATAAAATTAGC+AGG	-	chr2.2:4544636-4544655	MS.gene052050:CDS	35.0%
	TACTTCTCCAATGACTTCAA+TGG	-	chr2.2:4544275-4544294	MS.gene052050:CDS	35.0%
	TGAAGGTCTTGAAACAAAGA+GGG	+	chr2.2:4544721-4544740	None:intergenic	35.0%
	TTGAAGGTCTTGAAACAAAG+AGG	+	chr2.2:4544722-4544741	None:intergenic	35.0%
	TTGAAGTCATTGGAGAAGTA+GGG	+	chr2.2:4544275-4544294	None:intergenic	35.0%
!	AGAATCTTCCTTTAGTTCCA+TGG	-	chr2.2:4544442-4544461	MS.gene052050:CDS	35.0%
!	TGACCCCTGCTAATTTTATT+TGG	+	chr2.2:4544644-4544663	None:intergenic	35.0%
	AAATTAGCAGGGGTCAGTAT+TGG	-	chr2.2:4544648-4544667	MS.gene052050:CDS	40.0%
	TAGATGCAAAGCCACAGTAA+CGG	+	chr2.2:4544528-4544547	None:intergenic	40.0%
!	CTTCATGTTTTGTAGGGCTT+AGG	+	chr2.2:4544415-4544434	None:intergenic	40.0%
!	TTACTGTGGCTTTGCATCTA+GGG	-	chr2.2:4544528-4544547	MS.gene052050:CDS	40.0%
!!	CCCCTGCTAATTTTATTTGG+TGG	+	chr2.2:4544641-4544660	None:intergenic	40.0%
	AAAGGAAGAGCTTCTCTTGG+AGG	+	chr2.2:4544386-4544405	None:intergenic	45.0%
	ACCCCTGCTCAGATTCTAAT+CGG	-	chr2.2:4544677-4544696	MS.gene052050:CDS	45.0%
	ACCGATTAGAATCTGAGCAG+GGG	+	chr2.2:4544681-4544700	None:intergenic	45.0%
	AGCAAAGGAAGAGCTTCTCT+TGG	+	chr2.2:4544389-4544408	None:intergenic	45.0%
	ATTAGAATCTGAGCAGGGGT+AGG	+	chr2.2:4544677-4544696	None:intergenic	45.0%
	GACCGATTAGAATCTGAGCA+GGG	+	chr2.2:4544682-4544701	None:intergenic	45.0%
	GTAGGGCTTAGGTTAAGCAA+AGG	+	chr2.2:4544404-4544423	None:intergenic	45.0%
	TTAGAATCTGAGCAGGGGTA+GGG	+	chr2.2:4544676-4544695	None:intergenic	45.0%
!	AGAGGGCATGAAAACTGTGT+TGG	+	chr2.2:4544704-4544723	None:intergenic	45.0%
!	AGATGATGATGCCGTTACTG+TGG	-	chr2.2:4544514-4544533	MS.gene052050:CDS	45.0%
!	GTTACTGTGGCTTTGCATCT+AGG	-	chr2.2:4544527-4544546	MS.gene052050:CDS	45.0%
	GGACCGATTAGAATCTGAGC+AGG	+	chr2.2:4544683-4544702	None:intergenic	50.0%
	TCTCTTGGAGGTGGAGATGA+AGG	+	chr2.2:4544374-4544393	None:intergenic	50.0%
	GGAAGAGCTTCTCTTGGAGG+TGG	+	chr2.2:4544383-4544402	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	4544260	4544772	4544260	ID=MS.gene052050
chr2.2	mRNA	4544260	4544772	4544260	ID=MS.gene052050.t1;Parent=MS.gene052050
chr2.2	exon	4544260	4544772	4544260	ID=MS.gene052050.t1.exon1;Parent=MS.gene052050.t1
chr2.2	CDS	4544260	4544772	4544260	ID=cds.MS.gene052050.t1;Parent=MS.gene052050.t1

Gene Sequence

>MS.gene052050

ATGAGTACTCATCCCTACTTCTCCAATGACTTCAATGGTTATTGGTTTGACCTAAATAACCCTCCATTCTATAATTTTCCTTATAGTAACAACATATCTTTTGCATCTTCTCCTTCATCTCCACCTCCAAGAGAAGCTCTTCCTTTGCTTAACCTAAGCCCTACAAAACATGAAGATCTTCTAGAATCTTCCTTTAGTTCCATGGAAATGATGAATATGAAGGAGAAAGAACTAAGCATATCATCAAATTCTTTAGATGATGATGCCGTTACTGTGGCTTTGCATCTAGGGCTTCCAAGTATAAGTTCATCTGATTTAGCTTCTTCAAATATTTACAAAGATGATGAAAAAGTAGTGACTGTTGATTCTTCTGAGTGTCCACCAAATAAAATTAGCAGGGGTCAGTATTGGATCCCTACCCCTGCTCAGATTCTAATCGGTCCAACACAGTTTTCATGCCCTCTTTGTTTCAAGACCTTCAATAGATACAACAACATGCAGGTATATATTTAA

Protein sequence

>MS.gene052050.t1

MSTHPYFSNDFNGYWFDLNNPPFYNFPYSNNISFASSPSSPPPREALPLLNLSPTKHEDLLESSFSSMEMMNMKEKELSISSNSLDDDAVTVALHLGLPSISSSDLASSNIYKDDEKVVTVDSSECPPNKISRGQYWIPTPAQILIGPTQFSCPLCFKTFNRYNNMQVYI