AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31131

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31131.t1 MTR_2g097410 96.501 743 26 0 1 743 1 743 0.0 1499
MS.gene31131.t1 MTR_4g064150 57.823 735 251 12 21 743 48 735 0.0 804
MS.gene31131.t1 MTR_4g064120 56.897 696 206 10 54 743 21 628 0.0 764
MS.gene31131.t1 MTR_4g064180 56.466 696 209 10 54 743 21 628 0.0 753
MS.gene31131.t1 MTR_4g064160 48.892 722 313 17 35 743 8 686 0.0 625
MS.gene31131.t1 MTR_7g062120 44.585 711 330 17 61 743 68 742 0.0 566
MS.gene31131.t1 MTR_4g064200 52.677 467 192 6 294 743 198 652 5.65e-167 496
MS.gene31131.t1 MTR_2g097467 52.328 451 205 4 295 743 214 656 4.55e-165 491
MS.gene31131.t1 MTR_2g097463 50.531 471 209 5 284 743 200 657 5.58e-163 486
MS.gene31131.t1 MTR_2g097473 50.855 468 209 5 284 743 202 656 1.52e-162 484
MS.gene31131.t1 MTR_2g097310 52.516 457 200 8 291 743 197 640 3.33e-159 476
MS.gene31131.t1 MTR_2g097350 51.902 447 197 9 302 743 209 642 4.27e-154 462
MS.gene31131.t1 MTR_2g097390 51.454 447 199 9 302 743 210 643 2.66e-153 460
MS.gene31131.t1 MTR_2g097380 47.936 436 170 8 310 743 183 563 6.44e-133 405
MS.gene31131.t1 MTR_2g082090 32.979 376 245 4 367 740 208 578 7.76e-67 231
MS.gene31131.t1 MTR_3g056110 32.021 381 242 8 367 740 171 541 1.27e-60 214
MS.gene31131.t1 MTR_4g133660 30.607 379 255 5 366 741 170 543 1.52e-60 213
MS.gene31131.t1 MTR_5g094450 33.505 388 231 10 367 740 157 531 5.11e-60 212
MS.gene31131.t1 MTR_5g097480 30.851 376 253 4 367 740 173 543 1.30e-59 211
MS.gene31131.t1 MTR_7g027190 31.472 394 235 6 366 740 295 672 4.11e-56 204
MS.gene31131.t1 MTR_1g029420 29.679 374 256 4 369 740 223 591 1.24e-54 198
MS.gene31131.t1 MTR_2g026250 28.059 474 312 9 272 740 146 595 2.08e-54 197
MS.gene31131.t1 MTR_3g089055 30.851 376 249 7 369 740 201 569 2.78e-54 197
MS.gene31131.t1 MTR_3g089055 30.851 376 249 7 369 740 196 564 3.59e-54 196
MS.gene31131.t1 MTR_7g074650 31.793 368 235 6 375 742 442 793 3.15e-52 195
MS.gene31131.t1 MTR_8g442410 32.642 386 238 8 366 740 150 524 1.47e-50 186
MS.gene31131.t1 MTR_3g065980 31.218 394 231 6 366 740 167 539 2.41e-50 185
MS.gene31131.t1 MTR_4g104020 29.950 404 246 10 356 740 134 519 4.02e-50 184
MS.gene31131.t1 MTR_3g022830 26.555 418 250 6 372 740 26 435 3.86e-44 165
MS.gene31131.t1 MTR_4g102790 29.199 387 252 10 367 742 288 663 2.75e-43 167
MS.gene31131.t1 MTR_4g076140 26.402 428 250 5 371 740 48 468 3.56e-43 163
MS.gene31131.t1 MTR_4g076020 27.851 377 231 7 375 743 88 431 2.83e-42 160
MS.gene31131.t1 MTR_1g069725 27.518 407 264 12 343 741 80 463 3.24e-41 157
MS.gene31131.t1 MTR_4g097080 29.474 380 256 7 369 742 131 504 1.58e-40 156
MS.gene31131.t1 MTR_2g089100 26.290 407 259 9 368 743 49 445 2.94e-38 149
MS.gene31131.t1 MTR_2g034260 28.030 396 255 11 362 740 201 583 1.71e-36 145
MS.gene31131.t1 MTR_2g034250 28.354 395 256 8 362 740 201 584 3.69e-36 144
MS.gene31131.t1 MTR_4g077760 26.509 381 255 10 368 740 191 554 1.47e-35 142
MS.gene31131.t1 MTR_5g015490 28.125 384 258 7 371 742 114 491 4.11e-34 137
MS.gene31131.t1 MTR_5g058860 26.253 419 271 12 346 741 94 497 7.06e-32 130
MS.gene31131.t1 MTR_2g034280 26.020 392 266 7 362 740 194 574 1.20e-31 131
MS.gene31131.t1 MTR_8g065750 82.353 68 12 0 27 94 38 105 1.52e-31 119
MS.gene31131.t1 MTR_1g096030 27.456 397 243 14 371 740 80 458 1.67e-30 125
MS.gene31131.t1 MTR_3g072710 26.024 415 259 13 356 741 105 500 1.85e-30 126
MS.gene31131.t1 MTR_7g109580 28.093 388 248 11 366 740 184 553 2.83e-30 126
MS.gene31131.t1 MTR_6g047750 26.963 382 250 12 372 740 296 661 1.38e-29 125
MS.gene31131.t1 MTR_7g057230 25.867 375 259 8 372 740 295 656 2.35e-29 124
MS.gene31131.t1 MTR_5g015950 21.934 424 276 8 368 743 32 448 6.58e-27 115
MS.gene31131.t1 MTR_3g053270 21.934 424 276 8 368 743 32 448 6.58e-27 115
MS.gene31131.t1 MTR_4g095500 22.803 421 274 9 368 743 56 470 9.39e-27 114
MS.gene31131.t1 MTR_1g086970 25.505 396 255 13 366 740 101 477 3.19e-26 113
MS.gene31131.t1 MTR_5g009080 29.048 210 142 2 371 580 56 258 6.55e-26 112
MS.gene31131.t1 MTR_5g009080 28.188 149 107 0 592 740 329 477 2.23e-17 86.3
MS.gene31131.t1 MTR_3g087300 83.929 56 9 0 335 390 153 208 6.13e-24 100
MS.gene31131.t1 MTR_2g099110 26.733 303 204 6 371 667 74 364 1.11e-23 105
MS.gene31131.t1 MTR_8g093070 24.121 398 263 16 359 743 127 498 1.78e-22 102
MS.gene31131.t1 MTR_8g020840 24.365 394 242 14 358 727 163 524 4.02e-17 85.9
MS.gene31131.t1 MTR_0092s0100 23.016 378 259 8 372 740 376 730 1.01e-16 85.1
MS.gene31131.t1 MTR_0092s0100 23.016 378 259 8 372 740 373 727 1.26e-16 84.7
MS.gene31131.t1 MTR_4g026485 24.458 323 215 8 376 691 274 574 2.30e-16 83.6
MS.gene31131.t1 MTR_8g077940 22.691 379 262 12 372 740 183 540 9.43e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31131.t1 AT1G07530 49.000 700 315 10 68 743 86 767 0.0 634
MS.gene31131.t1 AT2G29060 47.323 691 267 14 62 742 53 656 0.0 584
MS.gene31131.t1 AT2G29060 47.338 695 298 14 62 742 53 693 0.0 579
MS.gene31131.t1 AT1G07520 45.586 691 295 12 69 743 70 695 0.0 560
MS.gene31131.t1 AT1G07520 45.586 691 295 12 69 743 71 696 0.0 560
MS.gene31131.t1 AT2G29065 44.695 754 282 18 1 743 1 630 0.0 556
MS.gene31131.t1 AT1G07520 51.337 561 218 10 194 743 15 531 0.0 548
MS.gene31131.t1 AT1G07520 51.337 561 218 10 194 743 15 531 0.0 548
MS.gene31131.t1 AT2G37650 40.591 744 344 16 27 742 41 714 1.43e-171 510
MS.gene31131.t1 AT2G37650 40.591 744 344 16 27 742 29 702 2.41e-171 509
MS.gene31131.t1 AT3G46600 50.783 447 207 5 297 743 116 549 1.93e-158 470
MS.gene31131.t1 AT3G46600 50.783 447 207 5 297 743 148 581 3.90e-158 471
MS.gene31131.t1 AT5G59450 47.410 502 246 8 257 743 102 600 1.68e-157 470
MS.gene31131.t1 AT3G46600 55.053 376 166 2 368 743 1 373 1.68e-150 443
MS.gene31131.t1 AT1G50600 31.915 376 249 4 367 740 155 525 3.92e-60 212
MS.gene31131.t1 AT1G50600 31.915 376 249 4 367 740 226 596 1.74e-59 212
MS.gene31131.t1 AT1G50600 31.915 376 249 4 367 740 230 600 2.33e-59 211
MS.gene31131.t1 AT5G48150 28.967 397 276 3 344 740 99 489 1.60e-58 206
MS.gene31131.t1 AT5G48150 28.967 397 276 3 344 740 99 489 1.60e-58 206
MS.gene31131.t1 AT5G48150 28.967 397 276 3 344 740 99 489 1.60e-58 206
MS.gene31131.t1 AT5G48150 28.967 397 276 3 344 740 99 489 1.60e-58 206
MS.gene31131.t1 AT2G04890 31.016 374 248 4 367 740 49 412 2.79e-58 204
MS.gene31131.t1 AT1G21450 29.825 456 303 9 292 740 147 592 5.05e-58 207
MS.gene31131.t1 AT1G66350 33.245 379 226 9 366 740 150 505 7.94e-52 188
MS.gene31131.t1 AT3G54220 30.095 422 277 7 324 742 245 651 1.45e-51 191
MS.gene31131.t1 AT4G17230 31.842 380 250 5 366 742 153 526 3.19e-51 187
MS.gene31131.t1 AT4G17230 31.842 380 250 5 366 742 153 526 3.19e-51 187
MS.gene31131.t1 AT2G01570 31.217 378 241 8 366 740 219 580 6.20e-49 182
MS.gene31131.t1 AT3G03450 29.894 378 251 8 366 740 178 544 6.51e-49 181
MS.gene31131.t1 AT1G14920 31.746 378 239 8 366 740 167 528 1.71e-48 179
MS.gene31131.t1 AT5G66770 33.231 325 203 7 375 696 224 537 1.64e-47 178
MS.gene31131.t1 AT1G50420 26.047 430 250 8 371 740 57 478 3.93e-44 166
MS.gene31131.t1 AT5G17490 27.632 380 251 8 366 740 155 515 2.64e-42 162
MS.gene31131.t1 AT3G50650 30.294 340 212 7 381 711 185 508 1.90e-39 154
MS.gene31131.t1 AT1G63100 31.871 342 214 9 367 701 273 602 4.97e-39 154
MS.gene31131.t1 AT1G63100 31.871 342 214 9 367 701 248 577 5.55e-39 154
MS.gene31131.t1 AT5G41920 27.559 381 251 7 366 740 38 399 5.89e-39 149
MS.gene31131.t1 AT3G49950 28.900 391 248 12 371 742 30 409 4.88e-36 141
MS.gene31131.t1 AT5G52510 25.773 388 258 10 369 740 266 639 1.37e-35 144
MS.gene31131.t1 AT4G37650 26.463 393 262 9 370 742 145 530 1.01e-33 136
MS.gene31131.t1 AT1G55580 27.187 423 239 12 368 740 41 444 1.03e-30 126
MS.gene31131.t1 AT4G08250 25.581 430 288 18 326 740 64 476 1.04e-24 108
MS.gene31131.t1 AT3G13840 23.934 422 275 14 330 740 112 498 4.64e-18 88.6
MS.gene31131.t1 AT4G00150 23.704 405 257 13 351 740 186 553 2.35e-15 80.1
MS.gene31131.t1 AT5G67411 30.846 201 125 8 488 677 1 198 1.33e-11 65.1

Find 173 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCCTTCACATTATTGATTT+TGG 0.112468 2.4:-6338973 MS.gene31131:CDS
CATCAAAGACAAAATCTTTA+TGG 0.121774 2.4:+6338263 None:intergenic
CCAGTTTCTGGTGGTGATTC+TGG 0.150366 2.4:-6339948 MS.gene31131:CDS
TCACCTGATACACCAGTTTC+TGG 0.198731 2.4:-6339960 MS.gene31131:CDS
TTGAATGGTTTCCCAGTTTC+GGG 0.204080 2.4:+6338750 None:intergenic
TTTGTTCAATCCATAGTAAA+TGG 0.225738 2.4:-6338583 MS.gene31131:CDS
TGTTGTGTTCCTACTGATTC+TGG 0.235374 2.4:-6340017 MS.gene31131:CDS
ATCTTCAAAGGTAGAATCTT+TGG 0.257325 2.4:+6340235 None:intergenic
CCTCATTGCTCTTAATATTA+AGG 0.263212 2.4:+6338719 None:intergenic
TTCAAGTTCAAATTTGAATT+CGG 0.265372 2.4:-6339610 MS.gene31131:CDS
TTTGAATGGTTTCCCAGTTT+CGG 0.285892 2.4:+6338749 None:intergenic
AGGCCATACCATCCCGAAAC+TGG 0.299357 2.4:-6338762 MS.gene31131:CDS
CAGCAGCACTAAACATCTTT+TGG 0.300712 2.4:+6339100 None:intergenic
AGGAAGCATTCTTCTCCTTC+AGG 0.315645 2.4:-6339216 MS.gene31131:CDS
TGCCGGTCGAAAGCCAGCTT+GGG 0.317241 2.4:+6338855 None:intergenic
CTCAAACTGAAACCATGATT+TGG 0.321027 2.4:+6339894 None:intergenic
TATCAAACATTGCAGAATAA+TGG 0.321158 2.4:+6338512 None:intergenic
ATTATAAGACCCATTTACTA+TGG 0.323109 2.4:+6338573 None:intergenic
AACTTCAGAACTGCATCTTT+AGG 0.332688 2.4:+6338631 None:intergenic
AAATCGTTAAGGTTTGGATT+TGG 0.332771 2.4:+6340323 None:intergenic
AGTGGCAGGTTAGGAATCTA+AGG 0.333559 2.4:-6338357 MS.gene31131:CDS
AATCTTCTTCCATTAGAATC+TGG 0.335576 2.4:+6340180 None:intergenic
CCACCAGAAACTGGTGTATC+AGG 0.337940 2.4:+6339957 None:intergenic
TCAAGTTCAAATTTGAATTC+GGG 0.339297 2.4:-6339609 MS.gene31131:CDS
TCAAGATGGAGGGTGGTGTT+AGG 0.341116 2.4:-6339653 MS.gene31131:CDS
GGAATCTAAGGGCTGGCTTT+AGG 0.342541 2.4:-6338345 MS.gene31131:CDS
GCAGTTTAGAAAAGGGTTAG+AGG 0.342843 2.4:-6339778 MS.gene31131:CDS
TTCTTCTGAGACTAGGGTTA+TGG 0.353283 2.4:-6339835 MS.gene31131:CDS
TGTGGATTTGAGGACCCTTT+TGG 0.355547 2.4:-6339304 MS.gene31131:CDS
CAGTTTATTGTTCCAAATCA+TGG 0.358564 2.4:-6339906 MS.gene31131:CDS
AAATATCTGATATGTTTGAT+AGG 0.361784 2.4:-6339461 MS.gene31131:CDS
TACGTATGCTTCGGCTGGTT+GGG 0.371904 2.4:-6338200 MS.gene31131:CDS
ATGAATTGTTGAGGCAGATT+AGG 0.382955 2.4:-6339236 MS.gene31131:CDS
CAGCCGAAGCATACGTAATT+CGG 0.384019 2.4:+6338206 None:intergenic
ACATCAAGAGGGTGTTGATT+AGG 0.390520 2.4:+6340062 None:intergenic
GTTTCGAATCCGACAAAGTT+CGG 0.391177 2.4:+6340392 None:intergenic
CATACAAACAGTGGCAGGTT+AGG 0.391689 2.4:-6338366 MS.gene31131:CDS
CTGCCGGTCGAAAGCCAGCT+TGG 0.397922 2.4:+6338854 None:intergenic
ATTAAGGTCTTCAATTTGAA+TGG 0.400958 2.4:+6338735 None:intergenic
TTACGTATGCTTCGGCTGGT+TGG 0.401437 2.4:-6338201 MS.gene31131:CDS
CAAGATGGAGGGTGGTGTTA+GGG 0.405701 2.4:-6339652 MS.gene31131:CDS
CAATGTTTCCTTCGAATACA+AGG 0.408276 2.4:-6338782 MS.gene31131:CDS
ATGGTGAAATCGTTAAGGTT+TGG 0.408700 2.4:+6340317 None:intergenic
ATGCAGTTCTGAAGTTAATC+AGG 0.410047 2.4:-6338624 MS.gene31131:CDS
GAATTGGATGGGCCCACATA+AGG 0.412630 2.4:+6340287 None:intergenic
CACCATGACCAGCCCTTATG+TGG 0.420581 2.4:-6340300 MS.gene31131:CDS
CTGCCACTGTTTGTATGTCT+CGG 0.422026 2.4:+6338372 None:intergenic
CCAGAATCACCACCAGAAAC+TGG 0.422848 2.4:+6339948 None:intergenic
CTGTGTCTGCTAATGACAAT+AGG 0.426533 2.4:-6339266 MS.gene31131:CDS
ATTTGGATTCTTCTGAGACT+AGG 0.434021 2.4:-6339842 MS.gene31131:CDS
ATATATAAGCCAGATTCTAA+TGG 0.435212 2.4:-6340189 MS.gene31131:CDS
AGTTCAAATTCGAATTCAAA+TGG 0.435366 2.4:-6339579 MS.gene31131:CDS
TAATGGCCAGATTTAGAGAT+AGG 0.438554 2.4:-6338300 MS.gene31131:CDS
TAGGCAACTCCCACTGGATA+AGG 0.439045 2.4:-6338326 MS.gene31131:CDS
TTTGGATTCTTCTGAGACTA+GGG 0.441086 2.4:-6339841 MS.gene31131:CDS
ACTCAGGTTGGTGAGCTTGA+TGG 0.441788 2.4:-6339369 MS.gene31131:CDS
TCTTAGCCTATCTCTAAATC+TGG 0.445660 2.4:+6338294 None:intergenic
TTCCCAGTTTCGGGATGGTA+TGG 0.447770 2.4:+6338759 None:intergenic
CGCATGGTTGGTGCTGGTAC+CGG 0.452661 2.4:-6339144 MS.gene31131:CDS
AATTCCTGTGATCCTCAACT+TGG 0.460110 2.4:+6338885 None:intergenic
TTGTTCAATCCATAGTAAAT+GGG 0.464694 2.4:-6338582 MS.gene31131:CDS
TCTTTGATGAAGATAACAAC+TGG 0.466798 2.4:-6338249 MS.gene31131:CDS
TTTAGTGTCTCATGAGCCTA+AGG 0.471760 2.4:-6339691 MS.gene31131:CDS
TGGCTTTAGGCAACTCCCAC+TGG 0.476205 2.4:-6338332 MS.gene31131:CDS
ACCATGGTCATCGAATTTGT+AGG 0.476706 2.4:+6340367 None:intergenic
GCAGAGAGAATAGAGGAAAC+TGG 0.479525 2.4:-6338835 MS.gene31131:CDS
GATAACAACTGGATGCTTCA+AGG 0.480041 2.4:-6338238 MS.gene31131:CDS
TCATATCCCGTGAAAATGAA+TGG 0.481425 2.4:-6338477 MS.gene31131:CDS
AGAAGGAAACTGTGGATTTG+AGG 0.481509 2.4:-6339314 MS.gene31131:CDS
GCTATGGATCCGAACTTTGT+CGG 0.481965 2.4:-6340401 MS.gene31131:CDS
TGGTCATGGTGAAATCGTTA+AGG 0.482527 2.4:+6340312 None:intergenic
TGCCACTGTTTGTATGTCTC+GGG 0.483709 2.4:+6338373 None:intergenic
AATTGGATGGGCCCACATAA+GGG 0.491814 2.4:+6340288 None:intergenic
TGGCTGTGAACTGTTTGGTA+AGG 0.497180 2.4:-6338693 MS.gene31131:CDS
CCCACTGGATAAGGAGGTAA+TGG 0.498395 2.4:-6338317 MS.gene31131:CDS
CGAATTACGTATGCTTCGGC+TGG 0.503997 2.4:-6338205 MS.gene31131:CDS
TTGAAGCGTGTGGAAAAGAA+AGG 0.504187 2.4:+6338547 None:intergenic
AATATCTGATATGTTTGATA+GGG 0.504258 2.4:-6339460 MS.gene31131:CDS
GGAACACAACAATTGCTACT+AGG 0.506690 2.4:+6340029 None:intergenic
GGGTGTTGATTAGGTGAAAG+AGG 0.507344 2.4:+6340071 None:intergenic
TATTAAGAGCAATGAGGTAG+TGG 0.510345 2.4:-6338713 MS.gene31131:CDS
TTATGTGGGCCCATCCAATT+CGG 0.511208 2.4:-6340285 MS.gene31131:CDS
ACAACTGGATGCTTCAAGGT+TGG 0.515568 2.4:-6338234 MS.gene31131:CDS
GTTTCCTCTATTCTCTCTGC+CGG 0.517130 2.4:+6338838 None:intergenic
GTCTCATGAGCCTAAGGGTA+GGG 0.518003 2.4:-6339685 MS.gene31131:CDS
TCCCCAAGCTGGCTTTCGAC+CGG 0.518500 2.4:-6338857 MS.gene31131:CDS
CCATTACCTCCTTATCCAGT+GGG 0.519924 2.4:+6338317 None:intergenic
ACGAGTGGCTCCGAATTGGA+TGG 0.521140 2.4:+6340275 None:intergenic
GCCGCTGTGTGCTGAACAAA+AGG 0.522967 2.4:-6339415 MS.gene31131:CDS
AGGCCGAATTACGTATGCTT+CGG 0.526366 2.4:-6338209 MS.gene31131:CDS
CTCTGTGATGCATCACCTGA+AGG 0.529263 2.4:+6339201 None:intergenic
TCAGTAACATATCATTATGC+TGG 0.533464 2.4:+6338175 None:intergenic
AGGTGATGCATCACAGAGAA+TGG 0.535230 2.4:-6339196 MS.gene31131:CDS
TACCAAAATCAATAATGTGA+AGG 0.537288 2.4:+6338971 None:intergenic
GCGATTGAAGCGCGCATGGT+TGG 0.539201 2.4:-6339156 MS.gene31131:CDS
CATGAGCCTAAGGGTAGGGT+TGG 0.539617 2.4:-6339681 MS.gene31131:CDS
AAAGAAACAAGGGAGGAAGA+AGG 0.539726 2.4:-6339331 MS.gene31131:CDS
ACCAAAATCAATAATGTGAA+GGG 0.539883 2.4:+6338972 None:intergenic
TCAGTATGTTGAAGACTAAG+TGG 0.541144 2.4:+6340128 None:intergenic
GAAGCGCGCATGGTTGGTGC+TGG 0.541418 2.4:-6339150 MS.gene31131:CDS
GCCATTACCTCCTTATCCAG+TGG 0.541967 2.4:+6338316 None:intergenic
CCACCCAAGTTGAGGATCAC+AGG 0.542256 2.4:-6338889 MS.gene31131:CDS
CATATATAATATTTGTGTAC+CGG 0.543480 2.4:+6339125 None:intergenic
TAGGGTTGGAGTGATCAAGA+TGG 0.543815 2.4:-6339667 MS.gene31131:CDS
GGAATTGAGTATCCCCAAGC+TGG 0.546615 2.4:-6338868 MS.gene31131:CDS
GTATCTTATATGGTTTCCAG+TGG 0.547028 2.4:-6338951 MS.gene31131:CDS
GGTAGTGGCTGTGAACTGTT+TGG 0.549486 2.4:-6338698 MS.gene31131:CDS
AGTAATAGTAATATTAGTGA+TGG 0.551444 2.4:-6339873 MS.gene31131:CDS
GGATGGGCCCACATAAGGGC+TGG 0.552239 2.4:+6340292 None:intergenic
GGTTGGAGTGATCAAGATGG+AGG 0.554472 2.4:-6339664 MS.gene31131:CDS
TGAAGCGTGTGGAAAAGAAA+GGG 0.554500 2.4:+6338548 None:intergenic
CTTGTGCACAAAGAACCAAA+AGG 0.555209 2.4:+6339289 None:intergenic
CGAGACATACAAACAGTGGC+AGG 0.561859 2.4:-6338371 MS.gene31131:CDS
ACCATGACCAGCCCTTATGT+GGG 0.563485 2.4:-6340299 MS.gene31131:CDS
CAGGTTAGGAATCTAAGGGC+TGG 0.564666 2.4:-6338352 MS.gene31131:CDS
GGCCATACCATCCCGAAACT+GGG 0.564944 2.4:-6338761 MS.gene31131:CDS
ATTCCTGTGATCCTCAACTT+GGG 0.566789 2.4:+6338886 None:intergenic
TCGACCGGCAGAGAGAATAG+AGG 0.567756 2.4:-6338842 MS.gene31131:CDS
ACTGGTGTATCAGGTGAAAG+AGG 0.568315 2.4:+6339966 None:intergenic
ACTTAATAAGAATTGGCCAC+TGG 0.572363 2.4:+6338935 None:intergenic
CAAGAAAGTGACGATGAAGA+AGG 0.574577 2.4:-6339525 MS.gene31131:CDS
CTACAACATTCATAATCTCC+CGG 0.577244 2.4:+6338425 None:intergenic
ATGAATGTTGTAGCATGTGA+AGG 0.579064 2.4:-6338415 MS.gene31131:CDS
TTGTGCACAAAGAACCAAAA+GGG 0.580206 2.4:+6339290 None:intergenic
ATGGTTTCCCAGTTTCGGGA+TGG 0.581937 2.4:+6338754 None:intergenic
CTGTGTGCTGAACAAAAGGA+TGG 0.582319 2.4:-6339411 MS.gene31131:CDS
CAGGTTGGTGAGCTTGATGG+AGG 0.586798 2.4:-6339366 MS.gene31131:CDS
TTAGAGATAGGCTAAGAGAA+TGG 0.591729 2.4:-6338288 MS.gene31131:CDS
CCTGTGATCCTCAACTTGGG+TGG 0.597902 2.4:+6338889 None:intergenic
TGGTGAGCTTGATGGAGGGA+AGG 0.598425 2.4:-6339361 MS.gene31131:CDS
GGAATCTCAAGAACATCAAG+AGG 0.598639 2.4:+6340050 None:intergenic
TGGATGCTTCAAGGTTGGAA+AGG 0.598660 2.4:-6338229 MS.gene31131:CDS
CGAGTGGCTCCGAATTGGAT+GGG 0.599028 2.4:+6340276 None:intergenic
GTTGGAGTGATCAAGATGGA+GGG 0.607035 2.4:-6339663 MS.gene31131:CDS
GAACTATTACCAGAATCAGT+AGG 0.607462 2.4:+6340008 None:intergenic
GGAGTGATCAAGATGGAGGG+TGG 0.608714 2.4:-6339660 MS.gene31131:CDS
AAGTGACTCCTTGAAGCGTG+TGG 0.608768 2.4:+6338537 None:intergenic
GAATGCGATTGAAGCGCGCA+TGG 0.609128 2.4:-6339160 MS.gene31131:CDS
TGTCTCATGAGCCTAAGGGT+AGG 0.616168 2.4:-6339686 MS.gene31131:CDS
CAGTATGTTGAAGACTAAGT+GGG 0.617757 2.4:+6340129 None:intergenic
GCCCACATAAGGGCTGGTCA+TGG 0.617923 2.4:+6340298 None:intergenic
TGGTATGGCCTTGTATTCGA+AGG 0.618500 2.4:+6338774 None:intergenic
AGATATTTCACTCTCCTCAA+CGG 0.618626 2.4:+6339476 None:intergenic
AGTGGTGGAAAGTAGAACTC+AGG 0.620773 2.4:-6339385 MS.gene31131:CDS
AAGGCACATACAAAGAAACA+AGG 0.622167 2.4:-6339342 MS.gene31131:CDS
GCCGGTCGAAAGCCAGCTTG+GGG 0.629257 2.4:+6338856 None:intergenic
GCAGTCTGCGGTTTCCGTTG+AGG 0.630810 2.4:-6339490 MS.gene31131:CDS
ACAAAAGGATGGATCAGTGG+TGG 0.631801 2.4:-6339400 MS.gene31131:CDS
AGGTTGGTGAGCTTGATGGA+GGG 0.634801 2.4:-6339365 MS.gene31131:CDS
AGAGAATAGAGGAAACTGGC+CGG 0.638860 2.4:-6338831 MS.gene31131:CDS
GCAACTCCCACTGGATAAGG+AGG 0.639677 2.4:-6338323 MS.gene31131:CDS
CACAATAGTTAGCTAAACGC+CGG 0.640868 2.4:+6338812 None:intergenic
ACCTACAAATTCGATGACCA+TGG 0.643298 2.4:-6340368 MS.gene31131:CDS
ATCACTCCAACCCTACCCTT+AGG 0.645813 2.4:+6339675 None:intergenic
TTAGTGTCTCATGAGCCTAA+GGG 0.648279 2.4:-6339690 MS.gene31131:CDS
GTGGCAGGTTAGGAATCTAA+GGG 0.650267 2.4:-6338356 MS.gene31131:CDS
AGAGAAGAATTAGATTACCA+TGG 0.658943 2.4:+6340351 None:intergenic
GGACTGCTAATGAATTGTTG+AGG 0.659007 2.4:-6339245 MS.gene31131:CDS
CCTGATACACCAGTTTCTGG+TGG 0.666329 2.4:-6339957 MS.gene31131:CDS
GTGGAAAGTAGAACTCAGGT+TGG 0.667091 2.4:-6339381 MS.gene31131:CDS
AGTGGGTCATAACACATGCA+TGG 0.667506 2.4:+6340146 None:intergenic
AGGGTGGACCACCCAAGTTG+AGG 0.670566 2.4:-6338897 MS.gene31131:CDS
GAAGCGTGTGGAAAAGAAAG+GGG 0.671904 2.4:+6338549 None:intergenic
GGCCCGAGACATACAAACAG+TGG 0.676458 2.4:-6338375 MS.gene31131:CDS
CCTTAATATTAAGAGCAATG+AGG 0.678676 2.4:-6338719 MS.gene31131:CDS
AAGAAGTAACAAGCAGTCTG+CGG 0.680051 2.4:-6339502 MS.gene31131:CDS
GAATCTCAAGAACATCAAGA+GGG 0.681038 2.4:+6340051 None:intergenic
CACATACAAAGAAACAAGGG+AGG 0.681274 2.4:-6339338 MS.gene31131:CDS
ATGGATTGAACAAATATGCG+CGG 0.684340 2.4:+6338592 None:intergenic
AGGGAGGAAGAAGGAAACTG+TGG 0.685784 2.4:-6339322 MS.gene31131:CDS
TGAACAAAAGGATGGATCAG+TGG 0.692488 2.4:-6339403 MS.gene31131:CDS
AGGCACATACAAAGAAACAA+GGG 0.704355 2.4:-6339341 MS.gene31131:CDS
TATCCCGTGAAAATGAATGG+AGG 0.724072 2.4:-6338474 MS.gene31131:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATCTGATATGTTTGAT+AGG - chr2.4:6339126-6339145 MS.gene31131:CDS 20.0%
!! AATATCTGATATGTTTGATA+GGG - chr2.4:6339127-6339146 MS.gene31131:CDS 20.0%
!! AGTAATAGTAATATTAGTGA+TGG - chr2.4:6338714-6338733 MS.gene31131:CDS 20.0%
!! CATATATAATATTTGTGTAC+CGG + chr2.4:6339465-6339484 None:intergenic 20.0%
!! GACAAAAACTTAATAAGAAT+TGG + chr2.4:6339662-6339681 None:intergenic 20.0%
!!! AAATGTGCAAATTTTTTGAA+AGG + chr2.4:6339551-6339570 None:intergenic 20.0%
!!! AATGTGCAAATTTTTTGAAA+GGG + chr2.4:6339550-6339569 None:intergenic 20.0%
!!! ATTGATTTTGGTATCTTATA+TGG - chr2.4:6339626-6339645 MS.gene31131:CDS 20.0%
!!! TTCAAGTTCAAATTTGAATT+CGG - chr2.4:6338977-6338996 MS.gene31131:CDS 20.0%
! ACCAAAATCAATAATGTGAA+GGG + chr2.4:6339618-6339637 None:intergenic 25.0%
! AGTTCAAATTCGAATTCAAA+TGG - chr2.4:6339008-6339027 MS.gene31131:CDS 25.0%
! ATATATAAGCCAGATTCTAA+TGG - chr2.4:6338398-6338417 MS.gene31131:CDS 25.0%
! ATTAAGGTCTTCAATTTGAA+TGG + chr2.4:6339855-6339874 None:intergenic 25.0%
! ATTATAAGACCCATTTACTA+TGG + chr2.4:6340017-6340036 None:intergenic 25.0%
! CATCAAAGACAAAATCTTTA+TGG + chr2.4:6340327-6340346 None:intergenic 25.0%
! TACCAAAATCAATAATGTGA+AGG + chr2.4:6339619-6339638 None:intergenic 25.0%
! TATCAAACATTGCAGAATAA+TGG + chr2.4:6340078-6340097 None:intergenic 25.0%
! TCAAGTTCAAATTTGAATTC+GGG - chr2.4:6338978-6338997 MS.gene31131:CDS 25.0%
! TTGTTCAATCCATAGTAAAT+GGG - chr2.4:6340005-6340024 MS.gene31131:CDS 25.0%
! TTTGTTCAATCCATAGTAAA+TGG - chr2.4:6340004-6340023 MS.gene31131:CDS 25.0%
!! AAGTTTTTGTCAAAAGTAGA+GGG - chr2.4:6339671-6339690 MS.gene31131:CDS 25.0%
!! CTATTTTGCAGTTTAGAAAA+GGG - chr2.4:6338802-6338821 MS.gene31131:CDS 25.0%
!! GAAAATTTTCTTTGATGCTT+TGG - chr2.4:6338482-6338501 MS.gene31131:CDS 25.0%
!! TAAGTTTTTGTCAAAAGTAG+AGG - chr2.4:6339670-6339689 MS.gene31131:CDS 25.0%
!! TCTATTTTGCAGTTTAGAAA+AGG - chr2.4:6338801-6338820 MS.gene31131:CDS 25.0%
AAATCGTTAAGGTTTGGATT+TGG + chr2.4:6338267-6338286 None:intergenic 30.0%
AATCTTCTTCCATTAGAATC+TGG + chr2.4:6338410-6338429 None:intergenic 30.0%
AGAGAAGAATTAGATTACCA+TGG + chr2.4:6338239-6338258 None:intergenic 30.0%
ATCTTCAAAGGTAGAATCTT+TGG + chr2.4:6338355-6338374 None:intergenic 30.0%
CAGTTTATTGTTCCAAATCA+TGG - chr2.4:6338681-6338700 MS.gene31131:CDS 30.0%
CCTCATTGCTCTTAATATTA+AGG + chr2.4:6339871-6339890 None:intergenic 30.0%
CCTTAATATTAAGAGCAATG+AGG - chr2.4:6339868-6339887 MS.gene31131:CDS 30.0%
TCTTTGATGAAGATAACAAC+TGG - chr2.4:6340338-6340357 MS.gene31131:CDS 30.0%
TGAAAAATCAGCATCTTCAA+AGG + chr2.4:6338367-6338386 None:intergenic 30.0%
! ATGATTGAGAGAGAGTTTTT+GGG - chr2.4:6340139-6340158 MS.gene31131:CDS 30.0%
!! GATTTTGAAAACAGCTGAAA+AGG - chr2.4:6339586-6339605 MS.gene31131:CDS 30.0%
!! TAGTGATGGTGTTTTTGATT+TGG - chr2.4:6338728-6338747 MS.gene31131:CDS 30.0%
!!! ACCCTTCACATTATTGATTT+TGG - chr2.4:6339614-6339633 MS.gene31131:CDS 30.0%
AACTTCAGAACTGCATCTTT+AGG + chr2.4:6339959-6339978 None:intergenic 35.0%
AAGGCACATACAAAGAAACA+AGG - chr2.4:6339245-6339264 MS.gene31131:CDS 35.0%
ACTTAATAAGAATTGGCCAC+TGG + chr2.4:6339655-6339674 None:intergenic 35.0%
AGATATTTCACTCTCCTCAA+CGG + chr2.4:6339114-6339133 None:intergenic 35.0%
AGGCACATACAAAGAAACAA+GGG - chr2.4:6339246-6339265 MS.gene31131:CDS 35.0%
ATGAATGTTGTAGCATGTGA+AGG - chr2.4:6340172-6340191 MS.gene31131:CDS 35.0%
ATGAATTGTTGAGGCAGATT+AGG - chr2.4:6339351-6339370 MS.gene31131:CDS 35.0%
ATGGTGAAATCGTTAAGGTT+TGG + chr2.4:6338273-6338292 None:intergenic 35.0%
CAATGTTTCCTTCGAATACA+AGG - chr2.4:6339805-6339824 MS.gene31131:CDS 35.0%
CAGTATGTTGAAGACTAAGT+GGG + chr2.4:6338461-6338480 None:intergenic 35.0%
CTACAACATTCATAATCTCC+CGG + chr2.4:6340165-6340184 None:intergenic 35.0%
CTCAAACTGAAACCATGATT+TGG + chr2.4:6338696-6338715 None:intergenic 35.0%
GAACTATTACCAGAATCAGT+AGG + chr2.4:6338582-6338601 None:intergenic 35.0%
GAATCTCAAGAACATCAAGA+GGG + chr2.4:6338539-6338558 None:intergenic 35.0%
GTATCTTATATGGTTTCCAG+TGG - chr2.4:6339636-6339655 MS.gene31131:CDS 35.0%
TAATGGCCAGATTTAGAGAT+AGG - chr2.4:6340287-6340306 MS.gene31131:CDS 35.0%
TATTAAGAGCAATGAGGTAG+TGG - chr2.4:6339874-6339893 MS.gene31131:CDS 35.0%
TCAGTATGTTGAAGACTAAG+TGG + chr2.4:6338462-6338481 None:intergenic 35.0%
TCATATCCCGTGAAAATGAA+TGG - chr2.4:6340110-6340129 MS.gene31131:CDS 35.0%
TCTTAGCCTATCTCTAAATC+TGG + chr2.4:6340296-6340315 None:intergenic 35.0%
TTAGAGATAGGCTAAGAGAA+TGG - chr2.4:6340299-6340318 MS.gene31131:CDS 35.0%
TTGTGCACAAAGAACCAAAA+GGG + chr2.4:6339300-6339319 None:intergenic 35.0%
TTTGAATGGTTTCCCAGTTT+CGG + chr2.4:6339841-6339860 None:intergenic 35.0%
! ATGGATTGAACAAATATGCG+CGG + chr2.4:6339998-6340017 None:intergenic 35.0%
! CAAAAAGCTCAGCTTTTTAC+TGG - chr2.4:6338852-6338871 MS.gene31131:CDS 35.0%
! GATGATTGAGAGAGAGTTTT+TGG - chr2.4:6340138-6340157 MS.gene31131:CDS 35.0%
! GTAAAAAGCTGAGCTTTTTG+AGG + chr2.4:6338852-6338871 None:intergenic 35.0%
! TTTTTGTCAAAAGTAGAGGG+TGG - chr2.4:6339674-6339693 MS.gene31131:CDS 35.0%
!! ATGCAGTTCTGAAGTTAATC+AGG - chr2.4:6339963-6339982 MS.gene31131:CDS 35.0%
!! ATTTGGATTCTTCTGAGACT+AGG - chr2.4:6338745-6338764 MS.gene31131:CDS 35.0%
!! TTTGGATTCTTCTGAGACTA+GGG - chr2.4:6338746-6338765 MS.gene31131:CDS 35.0%
AAAGAAACAAGGGAGGAAGA+AGG - chr2.4:6339256-6339275 MS.gene31131:CDS 40.0%
AAGAAGTAACAAGCAGTCTG+CGG - chr2.4:6339085-6339104 MS.gene31131:CDS 40.0%
AATTCCTGTGATCCTCAACT+TGG + chr2.4:6339705-6339724 None:intergenic 40.0%
ACCATGGTCATCGAATTTGT+AGG + chr2.4:6338223-6338242 None:intergenic 40.0%
ACCTACAAATTCGATGACCA+TGG - chr2.4:6338219-6338238 MS.gene31131:CDS 40.0%
AGAAGGAAACTGTGGATTTG+AGG - chr2.4:6339273-6339292 MS.gene31131:CDS 40.0%
ATTCCTGTGATCCTCAACTT+GGG + chr2.4:6339704-6339723 None:intergenic 40.0%
CAAGAAAGTGACGATGAAGA+AGG - chr2.4:6339062-6339081 MS.gene31131:CDS 40.0%
CACAATAGTTAGCTAAACGC+CGG + chr2.4:6339778-6339797 None:intergenic 40.0%
CACATACAAAGAAACAAGGG+AGG - chr2.4:6339249-6339268 MS.gene31131:CDS 40.0%
CTGTGTCTGCTAATGACAAT+AGG - chr2.4:6339321-6339340 MS.gene31131:CDS 40.0%
CTTGTGCACAAAGAACCAAA+AGG + chr2.4:6339301-6339320 None:intergenic 40.0%
GATAACAACTGGATGCTTCA+AGG - chr2.4:6340349-6340368 MS.gene31131:CDS 40.0%
GCAGTTTAGAAAAGGGTTAG+AGG - chr2.4:6338809-6338828 MS.gene31131:CDS 40.0%
GCATCAGAAGAAAAAACGAG+TGG + chr2.4:6338330-6338349 None:intergenic 40.0%
GGAACACAACAATTGCTACT+AGG + chr2.4:6338561-6338580 None:intergenic 40.0%
GGAATCTCAAGAACATCAAG+AGG + chr2.4:6338540-6338559 None:intergenic 40.0%
GGACTGCTAATGAATTGTTG+AGG - chr2.4:6339342-6339361 MS.gene31131:CDS 40.0%
GGCAAGCAGAGATAAAAACT+TGG + chr2.4:6339529-6339548 None:intergenic 40.0%
GTTTCGAATCCGACAAAGTT+CGG + chr2.4:6338198-6338217 None:intergenic 40.0%
TATCCCGTGAAAATGAATGG+AGG - chr2.4:6340113-6340132 MS.gene31131:CDS 40.0%
TGAACAAAAGGATGGATCAG+TGG - chr2.4:6339184-6339203 MS.gene31131:CDS 40.0%
TGAAGCGTGTGGAAAAGAAA+GGG + chr2.4:6340042-6340061 None:intergenic 40.0%
TGGTCATGGTGAAATCGTTA+AGG + chr2.4:6338278-6338297 None:intergenic 40.0%
TTAGTGTCTCATGAGCCTAA+GGG - chr2.4:6338897-6338916 MS.gene31131:CDS 40.0%
TTGAAGCGTGTGGAAAAGAA+AGG + chr2.4:6340043-6340062 None:intergenic 40.0%
TTGAATGGTTTCCCAGTTTC+GGG + chr2.4:6339840-6339859 None:intergenic 40.0%
TTTAGTGTCTCATGAGCCTA+AGG - chr2.4:6338896-6338915 MS.gene31131:CDS 40.0%
! CAGCAGCACTAAACATCTTT+TGG + chr2.4:6339490-6339509 None:intergenic 40.0%
! TCGACCTCCATTCATTTTCA+CGG + chr2.4:6340120-6340139 None:intergenic 40.0%
! TTTCTTTTCCACACGCTTCA+AGG - chr2.4:6340042-6340061 MS.gene31131:CDS 40.0%
!! ACATCAAGAGGGTGTTGATT+AGG + chr2.4:6338528-6338547 None:intergenic 40.0%
!! TGTTGTGTTCCTACTGATTC+TGG - chr2.4:6338570-6338589 MS.gene31131:CDS 40.0%
!! TTCTTCTGAGACTAGGGTTA+TGG - chr2.4:6338752-6338771 MS.gene31131:CDS 40.0%
!!! AGCATGTGAAGGTTTTGAGA+GGG - chr2.4:6340183-6340202 MS.gene31131:CDS 40.0%
!!! TAGCATGTGAAGGTTTTGAG+AGG - chr2.4:6340182-6340201 MS.gene31131:CDS 40.0%
AAAAACGAGTGGCTCCGAAT+TGG + chr2.4:6338319-6338338 None:intergenic 45.0%
AATTGGATGGGCCCACATAA+GGG + chr2.4:6338302-6338321 None:intergenic 45.0%
ACAAAAGGATGGATCAGTGG+TGG - chr2.4:6339187-6339206 MS.gene31131:CDS 45.0%
ACAACTGGATGCTTCAAGGT+TGG - chr2.4:6340353-6340372 MS.gene31131:CDS 45.0%
ACTGGTGTATCAGGTGAAAG+AGG + chr2.4:6338624-6338643 None:intergenic 45.0%
AGAGAATAGAGGAAACTGGC+CGG - chr2.4:6339756-6339775 MS.gene31131:CDS 45.0%
AGGCCGAATTACGTATGCTT+CGG - chr2.4:6340378-6340397 MS.gene31131:CDS 45.0%
AGGTGATGCATCACAGAGAA+TGG - chr2.4:6339391-6339410 MS.gene31131:CDS 45.0%
AGTGGCAGGTTAGGAATCTA+AGG - chr2.4:6340230-6340249 MS.gene31131:CDS 45.0%
AGTGGGTCATAACACATGCA+TGG + chr2.4:6338444-6338463 None:intergenic 45.0%
CAGCCGAAGCATACGTAATT+CGG + chr2.4:6340384-6340403 None:intergenic 45.0%
CATACAAACAGTGGCAGGTT+AGG - chr2.4:6340221-6340240 MS.gene31131:CDS 45.0%
CCATTACCTCCTTATCCAGT+GGG + chr2.4:6340273-6340292 None:intergenic 45.0%
CTGCCACTGTTTGTATGTCT+CGG + chr2.4:6340218-6340237 None:intergenic 45.0%
CTGTGTGCTGAACAAAAGGA+TGG - chr2.4:6339176-6339195 MS.gene31131:CDS 45.0%
GAAGCGTGTGGAAAAGAAAG+GGG + chr2.4:6340041-6340060 None:intergenic 45.0%
GCAGAGAGAATAGAGGAAAC+TGG - chr2.4:6339752-6339771 MS.gene31131:CDS 45.0%
GTGGAAAGTAGAACTCAGGT+TGG - chr2.4:6339206-6339225 MS.gene31131:CDS 45.0%
GTGGCAGGTTAGGAATCTAA+GGG - chr2.4:6340231-6340250 MS.gene31131:CDS 45.0%
GTTGGAGTGATCAAGATGGA+GGG - chr2.4:6338924-6338943 MS.gene31131:CDS 45.0%
GTTTCCTCTATTCTCTCTGC+CGG + chr2.4:6339752-6339771 None:intergenic 45.0%
TAGGGTTGGAGTGATCAAGA+TGG - chr2.4:6338920-6338939 MS.gene31131:CDS 45.0%
TCACCTGATACACCAGTTTC+TGG - chr2.4:6338627-6338646 MS.gene31131:CDS 45.0%
TGCCACTGTTTGTATGTCTC+GGG + chr2.4:6340217-6340236 None:intergenic 45.0%
TGGATGCTTCAAGGTTGGAA+AGG - chr2.4:6340358-6340377 MS.gene31131:CDS 45.0%
TGGCTGTGAACTGTTTGGTA+AGG - chr2.4:6339894-6339913 MS.gene31131:CDS 45.0%
TTATGTGGGCCCATCCAATT+CGG - chr2.4:6338302-6338321 MS.gene31131:CDS 45.0%
! AGTGGTGGAAAGTAGAACTC+AGG - chr2.4:6339202-6339221 MS.gene31131:CDS 45.0%
! CGACCTCCATTCATTTTCAC+GGG + chr2.4:6340119-6340138 None:intergenic 45.0%
! TCCTTTTGTTCAGCACACAG+CGG + chr2.4:6339176-6339195 None:intergenic 45.0%
! TGTGGATTTGAGGACCCTTT+TGG - chr2.4:6339283-6339302 MS.gene31131:CDS 45.0%
!! AGGAAGCATTCTTCTCCTTC+AGG - chr2.4:6339371-6339390 MS.gene31131:CDS 45.0%
!! GGGTGTTGATTAGGTGAAAG+AGG + chr2.4:6338519-6338538 None:intergenic 45.0%
!! TGGTATGGCCTTGTATTCGA+AGG + chr2.4:6339816-6339835 None:intergenic 45.0%
!! TTGAGAGAGAGTTTTTGGGC+CGG - chr2.4:6340143-6340162 MS.gene31131:CDS 45.0%
!!! AAGGTTTTGAGAGGGTTGAG+AGG - chr2.4:6340191-6340210 MS.gene31131:CDS 45.0%
AAGTGACTCCTTGAAGCGTG+TGG + chr2.4:6340053-6340072 None:intergenic 50.0%
ACCATGACCAGCCCTTATGT+GGG - chr2.4:6338288-6338307 MS.gene31131:CDS 50.0%
AGGGAGGAAGAAGGAAACTG+TGG - chr2.4:6339265-6339284 MS.gene31131:CDS 50.0%
ATCACTCCAACCCTACCCTT+AGG + chr2.4:6338915-6338934 None:intergenic 50.0%
ATGGTTTCCCAGTTTCGGGA+TGG + chr2.4:6339836-6339855 None:intergenic 50.0%
CAGGTTAGGAATCTAAGGGC+TGG - chr2.4:6340235-6340254 MS.gene31131:CDS 50.0%
CCACCAGAAACTGGTGTATC+AGG + chr2.4:6338633-6338652 None:intergenic 50.0%
CCAGAATCACCACCAGAAAC+TGG + chr2.4:6338642-6338661 None:intergenic 50.0%
CCCACTGGATAAGGAGGTAA+TGG - chr2.4:6340270-6340289 MS.gene31131:CDS 50.0%
CGAATTACGTATGCTTCGGC+TGG - chr2.4:6340382-6340401 MS.gene31131:CDS 50.0%
CGAGACATACAAACAGTGGC+AGG - chr2.4:6340216-6340235 MS.gene31131:CDS 50.0%
CTCTGTGATGCATCACCTGA+AGG + chr2.4:6339389-6339408 None:intergenic 50.0%
GAATTGGATGGGCCCACATA+AGG + chr2.4:6338303-6338322 None:intergenic 50.0%
GCCATTACCTCCTTATCCAG+TGG + chr2.4:6340274-6340293 None:intergenic 50.0%
GGAATTGAGTATCCCCAAGC+TGG - chr2.4:6339719-6339738 MS.gene31131:CDS 50.0%
GGTTGGAGTGATCAAGATGG+AGG - chr2.4:6338923-6338942 MS.gene31131:CDS 50.0%
GTCTCATGAGCCTAAGGGTA+GGG - chr2.4:6338902-6338921 MS.gene31131:CDS 50.0%
TAGGCAACTCCCACTGGATA+AGG - chr2.4:6340261-6340280 MS.gene31131:CDS 50.0%
TCAAGATGGAGGGTGGTGTT+AGG - chr2.4:6338934-6338953 MS.gene31131:CDS 50.0%
TGTCTCATGAGCCTAAGGGT+AGG - chr2.4:6338901-6338920 MS.gene31131:CDS 50.0%
TTACGTATGCTTCGGCTGGT+TGG - chr2.4:6340386-6340405 MS.gene31131:CDS 50.0%
TTCCCAGTTTCGGGATGGTA+TGG + chr2.4:6339831-6339850 None:intergenic 50.0%
! CAAGATGGAGGGTGGTGTTA+GGG - chr2.4:6338935-6338954 MS.gene31131:CDS 50.0%
! CCTGATACACCAGTTTCTGG+TGG - chr2.4:6338630-6338649 MS.gene31131:CDS 50.0%
! GGTAGTGGCTGTGAACTGTT+TGG - chr2.4:6339889-6339908 MS.gene31131:CDS 50.0%
! TACGTATGCTTCGGCTGGTT+GGG - chr2.4:6340387-6340406 MS.gene31131:CDS 50.0%
!! ACTCAGGTTGGTGAGCTTGA+TGG - chr2.4:6339218-6339237 MS.gene31131:CDS 50.0%
!! AGGTTGGTGAGCTTGATGGA+GGG - chr2.4:6339222-6339241 MS.gene31131:CDS 50.0%
!! CCAGTTTCTGGTGGTGATTC+TGG - chr2.4:6338639-6338658 MS.gene31131:CDS 50.0%
!! GGAATCTAAGGGCTGGCTTT+AGG - chr2.4:6340242-6340261 MS.gene31131:CDS 50.0%
!! TGAGAGAGAGTTTTTGGGCC+GGG - chr2.4:6340144-6340163 MS.gene31131:CDS 50.0%
ACGAGTGGCTCCGAATTGGA+TGG + chr2.4:6338315-6338334 None:intergenic 55.0%
AGGCCATACCATCCCGAAAC+TGG - chr2.4:6339825-6339844 MS.gene31131:CDS 55.0%
CACCATGACCAGCCCTTATG+TGG - chr2.4:6338287-6338306 MS.gene31131:CDS 55.0%
CATGAGCCTAAGGGTAGGGT+TGG - chr2.4:6338906-6338925 MS.gene31131:CDS 55.0%
CCTGTGATCCTCAACTTGGG+TGG + chr2.4:6339701-6339720 None:intergenic 55.0%
CGAGTGGCTCCGAATTGGAT+GGG + chr2.4:6338314-6338333 None:intergenic 55.0%
GAATGCGATTGAAGCGCGCA+TGG - chr2.4:6339427-6339446 MS.gene31131:CDS 55.0%
GCAACTCCCACTGGATAAGG+AGG - chr2.4:6340264-6340283 MS.gene31131:CDS 55.0%
GCCGCTGTGTGCTGAACAAA+AGG - chr2.4:6339172-6339191 MS.gene31131:CDS 55.0%
GGAGTGATCAAGATGGAGGG+TGG - chr2.4:6338927-6338946 MS.gene31131:CDS 55.0%
GGCCATACCATCCCGAAACT+GGG - chr2.4:6339826-6339845 MS.gene31131:CDS 55.0%
GGCCCGAGACATACAAACAG+TGG - chr2.4:6340212-6340231 MS.gene31131:CDS 55.0%
TCGACCGGCAGAGAGAATAG+AGG - chr2.4:6339745-6339764 MS.gene31131:CDS 55.0%
TGGTGAGCTTGATGGAGGGA+AGG - chr2.4:6339226-6339245 MS.gene31131:CDS 55.0%
! CCACCCAAGTTGAGGATCAC+AGG - chr2.4:6339698-6339717 MS.gene31131:CDS 55.0%
!! CAGGTTGGTGAGCTTGATGG+AGG - chr2.4:6339221-6339240 MS.gene31131:CDS 55.0%
!! TGGCTTTAGGCAACTCCCAC+TGG - chr2.4:6340255-6340274 MS.gene31131:CDS 55.0%
AGGGTGGACCACCCAAGTTG+AGG - chr2.4:6339690-6339709 MS.gene31131:CDS 60.0%
GCAGTCTGCGGTTTCCGTTG+AGG - chr2.4:6339097-6339116 MS.gene31131:CDS 60.0%
GCGATTGAAGCGCGCATGGT+TGG - chr2.4:6339431-6339450 MS.gene31131:CDS 60.0%
TGCCGGTCGAAAGCCAGCTT+GGG + chr2.4:6339735-6339754 None:intergenic 60.0%
!! CGCATGGTTGGTGCTGGTAC+CGG - chr2.4:6339443-6339462 MS.gene31131:CDS 60.0%
!! GCCCACATAAGGGCTGGTCA+TGG + chr2.4:6338292-6338311 None:intergenic 60.0%
!! TCCCCAAGCTGGCTTTCGAC+CGG - chr2.4:6339730-6339749 MS.gene31131:CDS 60.0%
CTGCCGGTCGAAAGCCAGCT+TGG + chr2.4:6339736-6339755 None:intergenic 65.0%
GCCGGTCGAAAGCCAGCTTG+GGG + chr2.4:6339734-6339753 None:intergenic 65.0%
GGATGGGCCCACATAAGGGC+TGG + chr2.4:6338298-6338317 None:intergenic 65.0%
! GAAGCGCGCATGGTTGGTGC+TGG - chr2.4:6339437-6339456 MS.gene31131:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 6338189 6340420 6338189 ID=MS.gene31131
chr2.4 mRNA 6338189 6340420 6338189 ID=MS.gene31131.t1;Parent=MS.gene31131
chr2.4 exon 6338189 6340420 6338189 ID=MS.gene31131.t1.exon1;Parent=MS.gene31131.t1
chr2.4 CDS 6338189 6340420 6338189 ID=cds.MS.gene31131.t1;Parent=MS.gene31131.t1
Gene Sequence

>MS.gene31131

ATGGATCCGAACTTTGTCGGATTCGAAACAACCTACAAATTCGATGACCATGGTAATCTAATTCTTCTCTCTGATCCAAATCCAAACCTTAACGATTTCACCATGACCAGCCCTTATGTGGGCCCATCCAATTCGGAGCCACTCGTTTTTTCTTCTGATGCAACCAAAGATTCTACCTTTGAAGATGCTGATTTTTCAGAAACTGTGAAATATATAAGCCAGATTCTAATGGAAGAAGATTTTGAACAAAAACCATGCATGTGTTATGACCCACTTAGTCTTCAACATACTGAGAAAATTTTCTTTGATGCTTTGGAATCAAATTTGCCTCTTTCACCTAATCAACACCCTCTTGATGTTCTTGAGATTCCTAGTAGCAATTGTTGTGTTCCTACTGATTCTGGTAATAGTTCTAGTTCTAATGAGTTAAAGCCTCTTTCACCTGATACACCAGTTTCTGGTGGTGATTCTGGTTTTAATTCAGTGAAGTCTCAGTTTATTGTTCCAAATCATGGTTTCAGTTTGAGTAATAGTAATATTAGTGATGGTGTTTTTGATTTGGATTCTTCTGAGACTAGGGTTATGGCTCAGAATATTTTTAGTGATGCTGATTCTATTTTGCAGTTTAGAAAAGGGTTAGAGGAAGCTAGCAAGTTTCTTCCTCAAAAAGCTCAGCTTTTTACTGGTTTTGAGAATGCTAGTGATAGTTTAGTGTCTCATGAGCCTAAGGGTAGGGTTGGAGTGATCAAGATGGAGGGTGGTGTTAGGGAGAACTCGAATTCGAGTTATTCAAGTTCAAATTTGAATTCGGGTTATTCGAGTTCAAATTCGAATTCAAATGGTTTGTTGAAGAGTAGAAAGAATCACGAGCGTCAAGAAAGTGACGATGAAGAAGGAAGAAGTAACAAGCAGTCTGCGGTTTCCGTTGAGGAGAGTGAAATATCTGATATGTTTGATAGGGTTTTGCTTAGTGTTGAAAACGTGCCGCTGTGTGCTGAACAAAAGGATGGATCAGTGGTGGAAAGTAGAACTCAGGTTGGTGAGCTTGATGGAGGGAAGGCACATACAAAGAAACAAGGGAGGAAGAAGGAAACTGTGGATTTGAGGACCCTTTTGGTTCTTTGTGCACAAGCTGTGTCTGCTAATGACAATAGGACTGCTAATGAATTGTTGAGGCAGATTAGGAAGCATTCTTCTCCTTCAGGTGATGCATCACAGAGAATGGCTCATTACTTTGCGAATGCGATTGAAGCGCGCATGGTTGGTGCTGGTACCGGTACACAAATATTATATATGAGCCAAAAGATGTTTAGTGCTGCTGATTTCTTGAAAGCTTACCAAGTTTTTATCTCTGCTTGCCCTTTCAAAAAATTTGCACATTTCTTTGCAAATAAAATGATTTTGAAAACAGCTGAAAAGGCAGAAACCCTTCACATTATTGATTTTGGTATCTTATATGGTTTCCAGTGGCCAATTCTTATTAAGTTTTTGTCAAAAGTAGAGGGTGGACCACCCAAGTTGAGGATCACAGGAATTGAGTATCCCCAAGCTGGCTTTCGACCGGCAGAGAGAATAGAGGAAACTGGCCGGCGTTTAGCTAACTATTGTGAACGTTTCAATGTTTCCTTCGAATACAAGGCCATACCATCCCGAAACTGGGAAACCATTCAAATTGAAGACCTTAATATTAAGAGCAATGAGGTAGTGGCTGTGAACTGTTTGGTAAGGTTTAAGAATTTACATGATGAGACAATCGACGTGAACAGTCCTAAAGATGCAGTTCTGAAGTTAATCAGGAAGATAAATCCGCGCATATTTGTTCAATCCATAGTAAATGGGTCTTATAATGCCCCTTTCTTTTCCACACGCTTCAAGGAGTCACTTTTCCATTATTCTGCAATGTTTGATATGTATGATACTCTCATATCCCGTGAAAATGAATGGAGGTCGATGATTGAGAGAGAGTTTTTGGGCCGGGAGATTATGAATGTTGTAGCATGTGAAGGTTTTGAGAGGGTTGAGAGGCCCGAGACATACAAACAGTGGCAGGTTAGGAATCTAAGGGCTGGCTTTAGGCAACTCCCACTGGATAAGGAGGTAATGGCCAGATTTAGAGATAGGCTAAGAGAATGGTACCATAAAGATTTTGTCTTTGATGAAGATAACAACTGGATGCTTCAAGGTTGGAAAGGCCGAATTACGTATGCTTCGGCTGGTTGGGTGCCAGCATAA
Protein sequence

>MS.gene31131.t1

MDPNFVGFETTYKFDDHGNLILLSDPNPNLNDFTMTSPYVGPSNSEPLVFSSDATKDSTFEDADFSETVKYISQILMEEDFEQKPCMCYDPLSLQHTEKIFFDALESNLPLSPNQHPLDVLEIPSSNCCVPTDSGNSSSSNELKPLSPDTPVSGGDSGFNSVKSQFIVPNHGFSLSNSNISDGVFDLDSSETRVMAQNIFSDADSILQFRKGLEEASKFLPQKAQLFTGFENASDSLVSHEPKGRVGVIKMEGGVRENSNSSYSSSNLNSGYSSSNSNSNGLLKSRKNHERQESDDEEGRSNKQSAVSVEESEISDMFDRVLLSVENVPLCAEQKDGSVVESRTQVGELDGGKAHTKKQGRKKETVDLRTLLVLCAQAVSANDNRTANELLRQIRKHSSPSGDASQRMAHYFANAIEARMVGAGTGTQILYMSQKMFSAADFLKAYQVFISACPFKKFAHFFANKMILKTAEKAETLHIIDFGILYGFQWPILIKFLSKVEGGPPKLRITGIEYPQAGFRPAERIEETGRRLANYCERFNVSFEYKAIPSRNWETIQIEDLNIKSNEVVAVNCLVRFKNLHDETIDVNSPKDAVLKLIRKINPRIFVQSIVNGSYNAPFFSTRFKESLFHYSAMFDMYDTLISRENEWRSMIEREFLGREIMNVVACEGFERVERPETYKQWQVRNLRAGFRQLPLDKEVMARFRDRLREWYHKDFVFDEDNNWMLQGWKGRITYASAGWVPA