Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32133.t1 | XP_013468646.1 | 77.6 | 299 | 63 | 2 | 1 | 296 | 1 | 298 | 3.90E-73 | 285 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32133.t1 | A0A072VML7 | 77.6 | 299 | 63 | 2 | 1 | 296 | 1 | 298 | 2.8e-73 | 285.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050901 | MS.gene32133 | 0.810238 | 1.27E-50 | -1.69E-46 |
| MS.gene05157 | MS.gene32133 | 0.8165 | 5.36E-52 | -1.69E-46 |
| MS.gene051720 | MS.gene32133 | 0.81492 | 1.21E-51 | -1.69E-46 |
| MS.gene051744 | MS.gene32133 | 0.801591 | 8.38E-49 | -1.69E-46 |
| MS.gene052732 | MS.gene32133 | 0.814526 | 1.47E-51 | -1.69E-46 |
| MS.gene053144 | MS.gene32133 | 0.832673 | 8.27E-56 | -1.69E-46 |
| MS.gene053581 | MS.gene32133 | 0.800375 | 1.48E-48 | -1.69E-46 |
| MS.gene054584 | MS.gene32133 | 0.824116 | 9.61E-54 | -1.69E-46 |
| MS.gene055580 | MS.gene32133 | -0.822429 | 2.38E-53 | -1.69E-46 |
| MS.gene060519 | MS.gene32133 | 0.821302 | 4.34E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32133.t1 | MTR_1g073670 | 93.980 | 299 | 14 | 2 | 1 | 296 | 1 | 298 | 2.84e-169 | 471 |
| MS.gene32133.t1 | MTR_1g069610 | 27.434 | 339 | 184 | 10 | 1 | 295 | 1 | 321 | 5.76e-14 | 72.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32133.t1 | AT2G33400 | 29.470 | 302 | 143 | 9 | 1 | 285 | 1 | 249 | 1.71e-24 | 100 |
| MS.gene32133.t1 | AT5G44040 | 32.095 | 296 | 144 | 11 | 30 | 295 | 71 | 339 | 7.11e-19 | 86.7 |
| MS.gene32133.t1 | AT1G04030 | 28.173 | 323 | 171 | 13 | 1 | 282 | 1 | 303 | 2.12e-12 | 67.4 |
| MS.gene32133.t1 | AT1G04030 | 28.173 | 323 | 171 | 13 | 1 | 282 | 1 | 303 | 2.12e-12 | 67.4 |
Find 45 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGAAAGTTTGGAGAATTA+TGG | 0.173513 | 1.2:-56807028 | MS.gene32133:CDS |
| CCCTAGTTCTTATCAGGTTT+TGG | 0.222472 | 1.2:-56807248 | MS.gene32133:CDS |
| GGCAAACCCTAGTTCTTATC+AGG | 0.299458 | 1.2:-56807254 | MS.gene32133:CDS |
| CGATGAGGAAGATGAAAATA+AGG | 0.325491 | 1.2:-56807218 | MS.gene32133:CDS |
| ACAAACCTGATTTCAGAATA+TGG | 0.325794 | 1.2:+56807451 | None:intergenic |
| TTGTACCATATTCTGAAATC+AGG | 0.328302 | 1.2:-56807456 | MS.gene32133:intron |
| ATGAAAGTTTGGAGAATTAT+GGG | 0.338370 | 1.2:-56807027 | MS.gene32133:CDS |
| TGATTGGGATGATGAAAGTT+TGG | 0.364371 | 1.2:-56807038 | MS.gene32133:CDS |
| GGAGGATGAAATAGTGTATG+AGG | 0.408167 | 1.2:-56807098 | MS.gene32133:CDS |
| ATGATGATGAATTTGATGAT+TGG | 0.418912 | 1.2:-56807054 | MS.gene32133:CDS |
| GAAGTTCTCGCCTTGCGTCT+TGG | 0.432670 | 1.2:-56806744 | MS.gene32133:CDS |
| TGATGATGAATTTGATGATT+GGG | 0.435554 | 1.2:-56807053 | MS.gene32133:CDS |
| TCAGTCGAAGCCTTTGTTGC+AGG | 0.437491 | 1.2:-56806708 | MS.gene32133:CDS |
| AACAGTGAACAGAAAGAAGA+TGG | 0.438966 | 1.2:-56806983 | MS.gene32133:CDS |
| TGCTAGTCTTTCAAACTGGT+TGG | 0.449139 | 1.2:-56806672 | MS.gene32133:CDS |
| CATTGAGTAAGATTCTCTAC+AGG | 0.451049 | 1.2:+56806872 | None:intergenic |
| AAGCATGCTGCCTGTAGTCT+TGG | 0.451334 | 1.2:-56806777 | MS.gene32133:CDS |
| CAACAAAGGCTTCGACTGAA+TGG | 0.464145 | 1.2:+56806712 | None:intergenic |
| TTGATGCTAGTCTTTCAAAC+TGG | 0.480004 | 1.2:-56806676 | MS.gene32133:CDS |
| TAGAGGTAGAGATCAAGAAA+AGG | 0.488908 | 1.2:-56807344 | MS.gene32133:CDS |
| TTTGAATGTAGAGATGTATG+AGG | 0.505130 | 1.2:-56807275 | MS.gene32133:CDS |
| ACTGCAACTTCCTGCAACAA+AGG | 0.507022 | 1.2:+56806698 | None:intergenic |
| ACTCTCTACAAAGTCCTAGC+TGG | 0.507971 | 1.2:-56807614 | MS.gene32133:CDS |
| GAGCTTGATTCCAAGACTAC+AGG | 0.512568 | 1.2:+56806767 | None:intergenic |
| CTGTAGAGAATCTTACTCAA+TGG | 0.515883 | 1.2:-56806871 | MS.gene32133:CDS |
| TATAATATCTATGATTCGAC+GGG | 0.526124 | 1.2:+56807143 | None:intergenic |
| TCCAAAACCTGATAAGAACT+AGG | 0.528369 | 1.2:+56807247 | None:intergenic |
| ATCAAGCAAACAAACGAGAA+AGG | 0.534474 | 1.2:-56806825 | MS.gene32133:CDS |
| TTATAATATCTATGATTCGA+CGG | 0.553522 | 1.2:+56807142 | None:intergenic |
| CCAAAACCTGATAAGAACTA+GGG | 0.562445 | 1.2:+56807248 | None:intergenic |
| GATCAAGAAAAGGAGAAGAG+TGG | 0.564885 | 1.2:-56807334 | MS.gene32133:CDS |
| TAGTTATGACAATGAGGAGG+AGG | 0.567704 | 1.2:-56807116 | MS.gene32133:CDS |
| ACAGTGAACAGAAAGAAGAT+GGG | 0.578317 | 1.2:-56806982 | MS.gene32133:CDS |
| CGAATTTGAGTGGACGAGAG+AGG | 0.606574 | 1.2:-56806922 | MS.gene32133:CDS |
| TCATTGACAACGAATTGTAG+TGG | 0.620784 | 1.2:+56806628 | None:intergenic |
| ATTGAGTAAGATTCTCTACA+GGG | 0.626097 | 1.2:+56806873 | None:intergenic |
| TGAAAATAAGGAGACTACTG+TGG | 0.644236 | 1.2:-56807206 | MS.gene32133:CDS |
| TAACTGTAGTTATGACAATG+AGG | 0.650246 | 1.2:-56807122 | MS.gene32133:CDS |
| GCATTCGATTCCAAGACGCA+AGG | 0.653152 | 1.2:+56806734 | None:intergenic |
| GAGTTGAAATCGAATTTGAG+TGG | 0.654782 | 1.2:-56806932 | MS.gene32133:CDS |
| GTAGTGGAGACATTATAACT+AGG | 0.665616 | 1.2:+56806644 | None:intergenic |
| ATTCTCTACAGGGTGCAGAA+CGG | 0.669664 | 1.2:+56806883 | None:intergenic |
| TGAAAGTTTGGAGAATTATG+GGG | 0.678615 | 1.2:-56807026 | MS.gene32133:CDS |
| ACTGTGGAATCAGAGAGAGA+AGG | 0.685984 | 1.2:-56807190 | MS.gene32133:CDS |
| CTGTAGTTATGACAATGAGG+AGG | 0.688122 | 1.2:-56807119 | MS.gene32133:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTATTTTATTTGTTAG+AGG | - | chr1.2:56806935-56806954 | MS.gene32133:CDS | 10.0% |
| !! | TGAAGAAATATTATTGTATG+TGG | - | chr1.2:56806887-56806906 | MS.gene32133:CDS | 20.0% |
| !! | TTATAATATCTATGATTCGA+CGG | + | chr1.2:56807157-56807176 | None:intergenic | 20.0% |
| ! | AATCAAGAACTTAAGAATGA+TGG | + | chr1.2:56806737-56806756 | None:intergenic | 25.0% |
| ! | ATCAAGAACTTAAGAATGAT+GGG | + | chr1.2:56806736-56806755 | None:intergenic | 25.0% |
| ! | ATGAAAGTTTGGAGAATTAT+GGG | - | chr1.2:56807269-56807288 | MS.gene32133:CDS | 25.0% |
| ! | TATAATATCTATGATTCGAC+GGG | + | chr1.2:56807156-56807175 | None:intergenic | 25.0% |
| !! | ATGATGATGAATTTGATGAT+TGG | - | chr1.2:56807242-56807261 | MS.gene32133:CDS | 25.0% |
| !! | CTTATCTTTTTGCAGAAATA+TGG | - | chr1.2:56806779-56806798 | MS.gene32133:CDS | 25.0% |
| !! | TGATGATGAATTTGATGATT+GGG | - | chr1.2:56807243-56807262 | MS.gene32133:CDS | 25.0% |
| !!! | GTTGTTTTGATATACTTACT+TGG | + | chr1.2:56806714-56806733 | None:intergenic | 25.0% |
| ACAAACCTGATTTCAGAATA+TGG | + | chr1.2:56806848-56806867 | None:intergenic | 30.0% | |
| ATTGAGTAAGATTCTCTACA+GGG | + | chr1.2:56807426-56807445 | None:intergenic | 30.0% | |
| GATGAAAGTTTGGAGAATTA+TGG | - | chr1.2:56807268-56807287 | MS.gene32133:CDS | 30.0% | |
| TAACTGTAGTTATGACAATG+AGG | - | chr1.2:56807174-56807193 | MS.gene32133:CDS | 30.0% | |
| TGAAAGTTTGGAGAATTATG+GGG | - | chr1.2:56807270-56807289 | MS.gene32133:CDS | 30.0% | |
| TTTGAATGTAGAGATGTATG+AGG | - | chr1.2:56807021-56807040 | MS.gene32133:CDS | 30.0% | |
| !! | TTGTACCATATTCTGAAATC+AGG | - | chr1.2:56806840-56806859 | MS.gene32133:CDS | 30.0% |
| AACAGTGAACAGAAAGAAGA+TGG | - | chr1.2:56807313-56807332 | MS.gene32133:CDS | 35.0% | |
| ACAGTGAACAGAAAGAAGAT+GGG | - | chr1.2:56807314-56807333 | MS.gene32133:CDS | 35.0% | |
| ATCAAGCAAACAAACGAGAA+AGG | - | chr1.2:56807471-56807490 | MS.gene32133:CDS | 35.0% | |
| CATTGAGTAAGATTCTCTAC+AGG | + | chr1.2:56807427-56807446 | None:intergenic | 35.0% | |
| CCAAAACCTGATAAGAACTA+GGG | + | chr1.2:56807051-56807070 | None:intergenic | 35.0% | |
| CGATGAGGAAGATGAAAATA+AGG | - | chr1.2:56807078-56807097 | MS.gene32133:CDS | 35.0% | |
| CTGTAGAGAATCTTACTCAA+TGG | - | chr1.2:56807425-56807444 | MS.gene32133:intron | 35.0% | |
| GAGTTGAAATCGAATTTGAG+TGG | - | chr1.2:56807364-56807383 | MS.gene32133:intron | 35.0% | |
| TAGAGGTAGAGATCAAGAAA+AGG | - | chr1.2:56806952-56806971 | MS.gene32133:CDS | 35.0% | |
| TCATTGACAACGAATTGTAG+TGG | + | chr1.2:56807671-56807690 | None:intergenic | 35.0% | |
| TCCAAAACCTGATAAGAACT+AGG | + | chr1.2:56807052-56807071 | None:intergenic | 35.0% | |
| TGAAAATAAGGAGACTACTG+TGG | - | chr1.2:56807090-56807109 | MS.gene32133:CDS | 35.0% | |
| TGATTGGGATGATGAAAGTT+TGG | - | chr1.2:56807258-56807277 | MS.gene32133:CDS | 35.0% | |
| ! | GTAGTGGAGACATTATAACT+AGG | + | chr1.2:56807655-56807674 | None:intergenic | 35.0% |
| !! | GGTTGTTTTCTTCAGTGTTT+TGG | - | chr1.2:56806631-56806650 | MS.gene32133:CDS | 35.0% |
| !! | TTGATGCTAGTCTTTCAAAC+TGG | - | chr1.2:56807620-56807639 | MS.gene32133:CDS | 35.0% |
| !!! | ATGCTTGTTTTTTGCTCTGA+TGG | + | chr1.2:56807505-56807524 | None:intergenic | 35.0% |
| CTGTAGTTATGACAATGAGG+AGG | - | chr1.2:56807177-56807196 | MS.gene32133:CDS | 40.0% | |
| GATCAAGAAAAGGAGAAGAG+TGG | - | chr1.2:56806962-56806981 | MS.gene32133:CDS | 40.0% | |
| GGAGGATGAAATAGTGTATG+AGG | - | chr1.2:56807198-56807217 | MS.gene32133:CDS | 40.0% | |
| TAGTTATGACAATGAGGAGG+AGG | - | chr1.2:56807180-56807199 | MS.gene32133:CDS | 40.0% | |
| ! | ATTGTATGTGGTTCTGTGAG+TGG | - | chr1.2:56806899-56806918 | MS.gene32133:CDS | 40.0% |
| !! | CCCTAGTTCTTATCAGGTTT+TGG | - | chr1.2:56807048-56807067 | MS.gene32133:CDS | 40.0% |
| !! | TGCTAGTCTTTCAAACTGGT+TGG | - | chr1.2:56807624-56807643 | MS.gene32133:CDS | 40.0% |
| !! | TTACTTGGTTTTCTCCAGCT+AGG | + | chr1.2:56806699-56806718 | None:intergenic | 40.0% |
| ACTCTCTACAAAGTCCTAGC+TGG | - | chr1.2:56806682-56806701 | MS.gene32133:CDS | 45.0% | |
| ACTGCAACTTCCTGCAACAA+AGG | + | chr1.2:56807601-56807620 | None:intergenic | 45.0% | |
| ACTGTGGAATCAGAGAGAGA+AGG | - | chr1.2:56807106-56807125 | MS.gene32133:CDS | 45.0% | |
| ATTCTCTACAGGGTGCAGAA+CGG | + | chr1.2:56807416-56807435 | None:intergenic | 45.0% | |
| CAACAAAGGCTTCGACTGAA+TGG | + | chr1.2:56807587-56807606 | None:intergenic | 45.0% | |
| GAGCTTGATTCCAAGACTAC+AGG | + | chr1.2:56807532-56807551 | None:intergenic | 45.0% | |
| ! | GGCAAACCCTAGTTCTTATC+AGG | - | chr1.2:56807042-56807061 | MS.gene32133:CDS | 45.0% |
| !!! | GGTTTTGGATGATGACGATG+AGG | - | chr1.2:56807063-56807082 | MS.gene32133:CDS | 45.0% |
| AAGCATGCTGCCTGTAGTCT+TGG | - | chr1.2:56807519-56807538 | MS.gene32133:intron | 50.0% | |
| CGAATTTGAGTGGACGAGAG+AGG | - | chr1.2:56807374-56807393 | MS.gene32133:intron | 50.0% | |
| GCATTCGATTCCAAGACGCA+AGG | + | chr1.2:56807565-56807584 | None:intergenic | 50.0% | |
| TCAGTCGAAGCCTTTGTTGC+AGG | - | chr1.2:56807588-56807607 | MS.gene32133:intron | 50.0% | |
| GAAGTTCTCGCCTTGCGTCT+TGG | - | chr1.2:56807552-56807571 | MS.gene32133:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 56806628 | 56807690 | 56806628 | ID=MS.gene32133 |
| chr1.2 | mRNA | 56806628 | 56807690 | 56806628 | ID=MS.gene32133.t1;Parent=MS.gene32133 |
| chr1.2 | exon | 56807604 | 56807690 | 56807604 | ID=MS.gene32133.t1.exon1;Parent=MS.gene32133.t1 |
| chr1.2 | CDS | 56807604 | 56807690 | 56807604 | ID=cds.MS.gene32133.t1;Parent=MS.gene32133.t1 |
| chr1.2 | exon | 56807457 | 56807524 | 56807457 | ID=MS.gene32133.t1.exon2;Parent=MS.gene32133.t1 |
| chr1.2 | CDS | 56807457 | 56807524 | 56807457 | ID=cds.MS.gene32133.t1;Parent=MS.gene32133.t1 |
| chr1.2 | exon | 56806628 | 56807363 | 56806628 | ID=MS.gene32133.t1.exon3;Parent=MS.gene32133.t1 |
| chr1.2 | CDS | 56806628 | 56807363 | 56806628 | ID=cds.MS.gene32133.t1;Parent=MS.gene32133.t1 |
| Gene Sequence |
| Protein sequence |
