AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32145

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32145.t1 MTR_1g073890 97.582 579 13 1 1 578 1 579 0.0 1160
MS.gene32145.t1 MTR_1g044135 69.156 616 145 10 1 578 1 609 0.0 831
MS.gene32145.t1 MTR_1g092540 35.777 573 302 11 36 552 16 578 2.95e-101 319
MS.gene32145.t1 MTR_6g461910 36.727 550 293 13 18 561 10 510 4.12e-91 290
MS.gene32145.t1 MTR_1g061540 35.009 537 299 13 29 547 17 521 1.63e-75 249
MS.gene32145.t1 MTR_7g103490 33.520 537 297 12 46 549 29 538 4.39e-73 244
MS.gene32145.t1 MTR_3g091180 61.006 159 57 2 405 558 422 580 2.96e-57 202
MS.gene32145.t1 MTR_3g091180 34.940 249 141 3 1 248 1 229 4.24e-38 149
MS.gene32145.t1 MTR_0318s0020 59.064 171 63 4 393 561 402 567 3.42e-53 191
MS.gene32145.t1 MTR_0318s0020 33.456 272 161 3 18 269 100 371 4.75e-34 137
MS.gene32145.t1 MTR_4g021260 43.458 214 112 2 44 248 17 230 6.78e-46 169
MS.gene32145.t1 MTR_4g021260 41.096 146 81 3 404 544 330 475 5.20e-29 120
MS.gene32145.t1 MTR_7g063040 41.475 217 121 1 38 248 17 233 8.97e-46 168
MS.gene32145.t1 MTR_7g063040 35.484 155 90 2 404 550 331 483 4.99e-25 108
MS.gene32145.t1 MTR_1g096970 32.701 211 133 3 46 250 51 258 2.11e-22 101
MS.gene32145.t1 MTR_1g096970 30.247 162 99 2 393 544 255 412 1.04e-14 77.4
MS.gene32145.t1 MTR_1g025340 28.807 243 156 5 46 280 127 360 4.92e-19 91.3
MS.gene32145.t1 MTR_1g025340 33.117 154 84 4 406 546 345 492 1.07e-13 74.3
MS.gene32145.t1 MTR_3g097240 29.954 217 147 4 46 261 141 353 2.28e-17 85.1
MS.gene32145.t1 MTR_3g097240 29.954 217 147 4 46 261 141 353 2.41e-17 85.1
MS.gene32145.t1 MTR_3g097240 29.954 217 147 4 46 261 141 353 4.30e-17 85.1
MS.gene32145.t1 MTR_3g114010 29.767 215 132 2 46 249 122 328 5.49e-17 84.7
MS.gene32145.t1 MTR_3g114010 32.911 158 91 3 406 552 340 493 3.34e-15 79.3
MS.gene32145.t1 MTR_8g095080 27.751 209 142 2 46 248 150 355 2.33e-12 69.7
MS.gene32145.t1 MTR_8g095080 28.230 209 141 2 46 248 150 355 2.61e-12 70.1
MS.gene32145.t1 MTR_8g095080 28.676 136 80 4 409 533 373 502 8.49e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32145.t1 AT3G23160 55.959 579 206 9 1 578 1 531 0.0 618
MS.gene32145.t1 AT5G04550 56.548 168 68 2 404 566 428 595 1.64e-56 200
MS.gene32145.t1 AT5G04550 34.025 241 158 1 35 274 14 254 2.53e-41 158
MS.gene32145.t1 AT5G04550 56.548 168 68 2 404 566 428 595 1.64e-56 200
MS.gene32145.t1 AT5G04550 34.025 241 158 1 35 274 14 254 2.53e-41 158
MS.gene32145.t1 AT5G51670 53.988 163 65 3 405 563 311 467 1.56e-48 176
MS.gene32145.t1 AT5G51670 30.038 263 162 2 1 250 1 254 4.52e-29 120
MS.gene32145.t1 AT5G51670 53.988 163 65 3 405 563 311 467 1.56e-48 176
MS.gene32145.t1 AT5G51670 30.038 263 162 2 1 250 1 254 4.52e-29 120
MS.gene32145.t1 AT1G34320 30.380 237 149 4 46 274 155 383 8.74e-22 100
MS.gene32145.t1 AT1G34320 30.719 153 88 4 405 544 373 520 9.18e-13 71.6
MS.gene32145.t1 AT1G34320 30.380 237 149 4 46 274 155 383 8.74e-22 100
MS.gene32145.t1 AT1G34320 30.719 153 88 4 405 544 373 520 9.18e-13 71.6
MS.gene32145.t1 AT1G30755 27.778 216 147 3 46 255 134 346 1.54e-14 76.6
MS.gene32145.t1 AT1G30755 27.778 216 147 3 46 255 134 346 1.55e-14 77.0
MS.gene32145.t1 AT1G30755 27.778 216 147 3 46 255 134 346 1.55e-14 77.0
MS.gene32145.t1 AT1G30755 27.778 216 147 3 46 255 134 346 1.55e-14 77.0
MS.gene32145.t1 AT1G30755 27.778 216 147 3 46 255 134 346 1.71e-14 76.3
MS.gene32145.t1 AT1G30755 27.778 216 147 3 46 255 134 346 1.71e-14 76.3
MS.gene32145.t1 AT5G08660 32.877 219 132 5 46 255 149 361 4.43e-12 68.9
MS.gene32145.t1 AT5G08660 33.491 212 126 5 46 248 149 354 4.70e-12 69.3
MS.gene32145.t1 AT5G08660 33.835 133 71 4 409 530 372 498 6.05e-12 68.9
MS.gene32145.t1 AT5G08660 33.491 212 126 5 46 248 149 354 4.70e-12 69.3
MS.gene32145.t1 AT5G08660 33.835 133 71 4 409 530 372 498 6.05e-12 68.9
MS.gene32145.t1 AT5G08660 33.491 212 126 5 46 248 149 354 4.70e-12 69.3
MS.gene32145.t1 AT5G08660 33.835 133 71 4 409 530 372 498 6.05e-12 68.9

Find 122 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAGAGGGTGTTAGGAATT+TGG 0.165683 1.2:-56967870 MS.gene32145:CDS
GCGTTGCTGGTGTGGTTTCT+AGG 0.206509 1.2:-56967784 MS.gene32145:CDS
TAGGAGTTTGTATAGTAAAA+TGG 0.207230 1.2:-56967609 MS.gene32145:CDS
TTTATAAAGTTGAATGGTTT+TGG 0.228524 1.2:+56967940 None:intergenic
GCTATATGTGAACTTCTTAA+AGG 0.229546 1.2:-56966519 MS.gene32145:CDS
CTTAGCTTTCCACATTTAAT+TGG 0.238050 1.2:-56966828 MS.gene32145:CDS
TAAGACATATGTCAAGAATT+TGG 0.242976 1.2:-56966736 MS.gene32145:CDS
ACAACAGCTAATCCACTATT+TGG 0.258196 1.2:+56966930 None:intergenic
TGGAAATATTGTCAAGAAAA+AGG 0.289545 1.2:-56966976 MS.gene32145:CDS
TATGTTCAACTTGAAGATTT+TGG 0.292464 1.2:-56967149 MS.gene32145:CDS
TCAAGCTCTCAAAGTCCTAT+TGG 0.298159 1.2:-56967002 MS.gene32145:CDS
GCTGGTGTGGTTTCTAGGTT+AGG 0.300286 1.2:-56967779 MS.gene32145:CDS
TATGCTCCTCCTTCTACTCT+TGG 0.307855 1.2:-56966906 MS.gene32145:CDS
CTTGACATATGTCTTAAGTT+TGG 0.310915 1.2:+56966743 None:intergenic
GTAGAAAACATGGTCCTAAA+AGG 0.314812 1.2:-56968024 MS.gene32145:CDS
CCCTTCTACTCTCCTCATTC+TGG 0.318915 1.2:+56967537 None:intergenic
TTTCCTTGTGGAACAAGTCC+TGG 0.333137 1.2:-56967125 MS.gene32145:CDS
ACAACAACAAAACGCATTAC+TGG 0.333831 1.2:-56966472 MS.gene32145:CDS
GGAAGGAATGGTGAGGAAAA+TGG 0.336491 1.2:-56967651 MS.gene32145:CDS
ATTTGGAAGATTGCATGAAA+TGG 0.336800 1.2:-56966431 MS.gene32145:CDS
GGTTACAATCTGAGTTCAAT+TGG 0.339082 1.2:-56967224 MS.gene32145:CDS
AGGAGTTTGTATAGTAAAAT+GGG 0.339974 1.2:-56967608 MS.gene32145:CDS
GCCAGAATGAGGAGAGTAGA+AGG 0.346143 1.2:-56967538 MS.gene32145:CDS
TTGTGCAAGCAGTTTCAATT+TGG 0.348203 1.2:-56966448 MS.gene32145:CDS
TAGAAGAAGTTTCAAGCAAA+AGG 0.356838 1.2:+56968050 None:intergenic
GTTATAGATGTGAATGAATT+GGG 0.361108 1.2:-56967692 MS.gene32145:CDS
GTTTAATATTTGCACTTACT+TGG 0.364850 1.2:+56968077 None:intergenic
GAGGAATTGAACCGCGTTGC+TGG 0.366993 1.2:-56967797 MS.gene32145:CDS
TGCAAGAGGGGCATCATATA+TGG 0.376522 1.2:+56966713 None:intergenic
TCTTGAACTCAGAGGGTGTT+AGG 0.377364 1.2:-56967877 MS.gene32145:CDS
GACTGGAAGGCGACTCTTGA+TGG 0.380397 1.2:-56966687 MS.gene32145:CDS
TGTTATAGATGTGAATGAAT+TGG 0.381186 1.2:-56967693 MS.gene32145:CDS
CTGAGTTCAATTGGGAGAAA+TGG 0.393596 1.2:-56967215 MS.gene32145:CDS
ACAATATTTCCAATCCCAAT+AGG 0.393961 1.2:+56966987 None:intergenic
AATAAGAAAGTAGAAACTAT+AGG 0.400565 1.2:-56967995 MS.gene32145:CDS
GCACATCCTCCAAGAGTAGA+AGG 0.403510 1.2:+56966897 None:intergenic
TGATAAGGTTGTTGAGTTGT+TGG 0.404841 1.2:-56967438 MS.gene32145:CDS
TCAACCTTTGGTCCAAATAG+TGG 0.405123 1.2:-56966942 MS.gene32145:CDS
CAAGCTCTCAAAGTCCTATT+GGG 0.410774 1.2:-56967001 MS.gene32145:CDS
AGTAAGTGCAAATATTAAAC+AGG 0.427740 1.2:-56968074 MS.gene32145:CDS
GGTTTGAGCAGAGGCTTGTA+TGG 0.433545 1.2:-56967514 MS.gene32145:CDS
ATGCCCCTCTTGCACATGAC+TGG 0.436871 1.2:-56966704 MS.gene32145:CDS
AATCTGAGATAGCAAAGTTA+AGG 0.437201 1.2:-56967907 MS.gene32145:CDS
TTGCTCATAATATGATGAAA+TGG 0.441213 1.2:-56966641 MS.gene32145:CDS
TAGCTTAAGGATATCTTTGA+AGG 0.441874 1.2:-56966766 MS.gene32145:CDS
CAATGATTTATAAAGTTGAA+TGG 0.441919 1.2:+56967934 None:intergenic
GAGCACTTATGCTCAACCTT+TGG 0.443046 1.2:-56966954 MS.gene32145:CDS
AGATGCTGCCGACTAGCTTA+AGG 0.443970 1.2:-56966779 MS.gene32145:CDS
GCAAGATGTGAGGCAGCTTA+AGG 0.445171 1.2:-56967486 MS.gene32145:CDS
GCTCCTCCTTCTACTCTTGG+AGG 0.447412 1.2:-56966903 MS.gene32145:CDS
CTGGTGTGGTTTCTAGGTTA+GGG 0.450011 1.2:-56967778 MS.gene32145:CDS
GCAAGAATCTGTATGGTCTT+TGG 0.454280 1.2:-56967392 MS.gene32145:CDS
TAGCTATGGTTGCTGAAGCT+TGG 0.455269 1.2:-56968117 None:intergenic
TGCTCAAAGCCTTGCAAAGC+AGG 0.457798 1.2:+56967740 None:intergenic
CAAGGCTTTGAGCATGTGTA+TGG 0.461482 1.2:-56967731 MS.gene32145:CDS
AATTGTGAAGGTTCGCCACT+TGG 0.471694 1.2:-56967335 MS.gene32145:CDS
AGAAGAAGTTTCAAGCAAAA+GGG 0.473384 1.2:+56968051 None:intergenic
CATACAGATTCTTGCATACA+AGG 0.476054 1.2:+56967400 None:intergenic
GTGTATGGTGATGTTGTTAG+TGG 0.486403 1.2:-56967716 MS.gene32145:CDS
CTTGTATGCAAGAATCTGTA+TGG 0.486624 1.2:-56967399 MS.gene32145:CDS
TGTTCTGTGCCTGCTTTGCA+AGG 0.488513 1.2:-56967749 MS.gene32145:CDS
GTTACAATCTGAGTTCAATT+GGG 0.489431 1.2:-56967223 MS.gene32145:CDS
TCTCAAAGTCCTATTGGGAT+TGG 0.494966 1.2:-56966996 MS.gene32145:CDS
CAGAATGAGGAGAGTAGAAG+GGG 0.495320 1.2:-56967536 MS.gene32145:CDS
AGTTAAGCATATGGAAGGAA+TGG 0.497345 1.2:-56967663 MS.gene32145:CDS
TTCCTTGTGGAACAAGTCCT+GGG 0.502257 1.2:-56967124 MS.gene32145:CDS
CAACAACAAAACGCATTACT+GGG 0.507166 1.2:-56966471 MS.gene32145:CDS
AGCTTGATACACAACTGTAT+TGG 0.514613 1.2:+56967018 None:intergenic
TGGGCAAAATGAATGTGGTT+CGG 0.525125 1.2:-56967315 MS.gene32145:CDS
TTGGGAGAAATGGTAGTAGT+AGG 0.529468 1.2:-56967205 MS.gene32145:CDS
AATTTGGTTTCTTCAGAAGA+GGG 0.530169 1.2:-56967854 MS.gene32145:CDS
ATAGGTATGTGAGTGCTACT+AGG 0.530403 1.2:-56967628 MS.gene32145:CDS
AGGAAGGATAGCCTTGTGAT+TGG 0.535732 1.2:-56967359 MS.gene32145:CDS
GTCTAGCTTGATTAATGTTG+AGG 0.536642 1.2:-56967291 MS.gene32145:CDS
AGCAACGCGGAATAGTTAGC+AGG 0.539520 1.2:-56966599 MS.gene32145:CDS
CTTTGGTGATTCTACGAGGA+AGG 0.541319 1.2:-56967375 MS.gene32145:CDS
CCCTCTTGCACATGACTGGA+AGG 0.541581 1.2:-56966700 MS.gene32145:CDS
TAATGTTGAGGTGAATTTGT+CGG 0.544358 1.2:-56967279 MS.gene32145:CDS
ACTGGAAGGCGACTCTTGAT+GGG 0.545261 1.2:-56966686 MS.gene32145:CDS
ATTGAACCGCGTTGCTGGTG+TGG 0.547538 1.2:-56967792 MS.gene32145:CDS
GAATTTGGTTTCTTCAGAAG+AGG 0.557080 1.2:-56967855 MS.gene32145:CDS
TAATCCACTATTTGGACCAA+AGG 0.563309 1.2:+56966938 None:intergenic
GGAGTTTGTATAGTAAAATG+GGG 0.565248 1.2:-56967607 MS.gene32145:CDS
CTTGTGATTGGTAATTGTGA+AGG 0.569987 1.2:-56967347 MS.gene32145:CDS
CCAGAATGAGGAGAGTAGAA+GGG 0.569991 1.2:-56967537 MS.gene32145:CDS
GAGTTTGTATAGTAAAATGG+GGG 0.573559 1.2:-56967606 MS.gene32145:CDS
CCTTCCAGTCATGTGCAAGA+GGG 0.574005 1.2:+56966700 None:intergenic
GCCTTCCAGTCATGTGCAAG+AGG 0.575283 1.2:+56966699 None:intergenic
CTCTTGATGGGATACTTCGG+TGG 0.577628 1.2:-56966674 MS.gene32145:CDS
TCAGAATAATAGCCAGAATG+AGG 0.581576 1.2:-56967549 MS.gene32145:CDS
AGGTTGTTGAGTTGTTGGCT+AGG 0.583359 1.2:-56967433 MS.gene32145:CDS
TTATAGATGTGAATGAATTG+GGG 0.583468 1.2:-56967691 MS.gene32145:CDS
ATTGTGAAGGTTCGCCACTT+GGG 0.585918 1.2:-56967334 MS.gene32145:CDS
TTCATCATATTATGAGCAAG+CGG 0.586075 1.2:+56966645 None:intergenic
GAATGGTGAGGAAAATGGAT+AGG 0.586857 1.2:-56967646 MS.gene32145:CDS
CTTTCCACATTTAATTGGCG+AGG 0.591587 1.2:-56966823 MS.gene32145:CDS
AATGAGATCTTGAACTCAGA+GGG 0.592154 1.2:-56967884 MS.gene32145:CDS
TGATGAAATGGCAAAGTGAG+AGG 0.594896 1.2:-56966629 MS.gene32145:CDS
CACAACTGTATTGGTCCTCA+CGG 0.597461 1.2:+56967027 None:intergenic
CATTCCATGAAAAGTCTCCC+AGG 0.598749 1.2:+56967107 None:intergenic
AAAGATATCCTTAAGCTAGT+CGG 0.600246 1.2:+56966771 None:intergenic
CGACTCTTGATGGGATACTT+CGG 0.600804 1.2:-56966677 MS.gene32145:CDS
CCACTTGGGCAAAATGAATG+TGG 0.605178 1.2:-56967320 MS.gene32145:CDS
CTTCCAGTCATGTGCAAGAG+GGG 0.606461 1.2:+56966701 None:intergenic
AGCTTAAGGAGATTTCACTT+TGG 0.607378 1.2:-56967472 MS.gene32145:CDS
GCTTCCTCGCCAATTAAATG+TGG 0.610343 1.2:+56966819 None:intergenic
CTCCCAGGACTTGTTCCACA+AGG 0.620495 1.2:+56967122 None:intergenic
CTTCACAATTACCAATCACA+AGG 0.641214 1.2:+56967348 None:intergenic
TCTTCTACTAGTAGAAAACA+TGG 0.643423 1.2:-56968034 MS.gene32145:CDS
AGCATATGGAAGGAATGGTG+AGG 0.646265 1.2:-56967658 MS.gene32145:CDS
AGTTTGTATAGTAAAATGGG+GGG 0.660331 1.2:-56967605 MS.gene32145:CDS
TCTCATATAGATATGAAGAG+AGG 0.666893 1.2:-56967182 MS.gene32145:CDS
TGCTGATAAGATAAAGACAG+AGG 0.679639 1.2:-56966544 MS.gene32145:CDS
CATCCTCCAAGAGTAGAAGG+AGG 0.691868 1.2:+56966900 None:intergenic
GAATGAGATCTTGAACTCAG+AGG 0.696161 1.2:-56967885 MS.gene32145:CDS
TGTTCGACGTGAGAAGCTCG+AGG 0.696638 1.2:-56967816 MS.gene32145:CDS
TGGTCTTTGGTGATTCTACG+AGG 0.706603 1.2:-56967379 MS.gene32145:CDS
TATGGCAGAAGCAAGATGTG+AGG 0.708787 1.2:-56967496 MS.gene32145:CDS
AGAATGAGGAGAGTAGAAGG+GGG 0.748505 1.2:-56967535 MS.gene32145:CDS
TAGAAACCACACCAGCAACG+CGG 0.763644 1.2:+56967786 None:intergenic
TGATGTTGCTATCGACCGTG+AGG 0.766347 1.2:-56967042 MS.gene32145:CDS
ATGAAATGGAAATTGCAGTG+CGG 0.848060 1.2:-56966417 MS.gene32145:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAGAAAGTAGAAACTAT+AGG - chr1.2:56966515-56966534 MS.gene32145:CDS 20.0%
!! CAATGATTTATAAAGTTGAA+TGG + chr1.2:56966579-56966598 None:intergenic 20.0%
!!! TTTATAAAGTTGAATGGTTT+TGG + chr1.2:56966573-56966592 None:intergenic 20.0%
! AGGAGTTTGTATAGTAAAAT+GGG - chr1.2:56966902-56966921 MS.gene32145:CDS 25.0%
! AGTAAGTGCAAATATTAAAC+AGG - chr1.2:56966436-56966455 MS.gene32145:CDS 25.0%
! GTTATAGATGTGAATGAATT+GGG - chr1.2:56966818-56966837 MS.gene32145:CDS 25.0%
! GTTTAATATTTGCACTTACT+TGG + chr1.2:56966436-56966455 None:intergenic 25.0%
! TAAGACATATGTCAAGAATT+TGG - chr1.2:56967774-56967793 MS.gene32145:CDS 25.0%
! TAGGAGTTTGTATAGTAAAA+TGG - chr1.2:56966901-56966920 MS.gene32145:CDS 25.0%
! TGGAAATATTGTCAAGAAAA+AGG - chr1.2:56967534-56967553 MS.gene32145:CDS 25.0%
! TGTTATAGATGTGAATGAAT+TGG - chr1.2:56966817-56966836 MS.gene32145:CDS 25.0%
! TTATAGATGTGAATGAATTG+GGG - chr1.2:56966819-56966838 MS.gene32145:CDS 25.0%
! TTGCTCATAATATGATGAAA+TGG - chr1.2:56967869-56967888 MS.gene32145:CDS 25.0%
!! TATGTTCAACTTGAAGATTT+TGG - chr1.2:56967361-56967380 MS.gene32145:CDS 25.0%
!! TTCTTATTCTCTTTCCTTTT+AGG + chr1.2:56966503-56966522 None:intergenic 25.0%
!!! TTTTTAGTTAAGCATATGGA+AGG - chr1.2:56966842-56966861 MS.gene32145:CDS 25.0%
AAAGATATCCTTAAGCTAGT+CGG + chr1.2:56967742-56967761 None:intergenic 30.0%
ACAATATTTCCAATCCCAAT+AGG + chr1.2:56967526-56967545 None:intergenic 30.0%
AGAAGAAGTTTCAAGCAAAA+GGG + chr1.2:56966462-56966481 None:intergenic 30.0%
AGTTTGTATAGTAAAATGGG+GGG - chr1.2:56966905-56966924 MS.gene32145:CDS 30.0%
ATTTGGAAGATTGCATGAAA+TGG - chr1.2:56968079-56968098 MS.gene32145:CDS 30.0%
CTTGACATATGTCTTAAGTT+TGG + chr1.2:56967770-56967789 None:intergenic 30.0%
GAGTTTGTATAGTAAAATGG+GGG - chr1.2:56966904-56966923 MS.gene32145:CDS 30.0%
GCTATATGTGAACTTCTTAA+AGG - chr1.2:56967991-56968010 MS.gene32145:CDS 30.0%
GGAGTTTGTATAGTAAAATG+GGG - chr1.2:56966903-56966922 MS.gene32145:CDS 30.0%
GTTACAATCTGAGTTCAATT+GGG - chr1.2:56967287-56967306 MS.gene32145:CDS 30.0%
TAATGTTGAGGTGAATTTGT+CGG - chr1.2:56967231-56967250 MS.gene32145:CDS 30.0%
TAGAAGAAGTTTCAAGCAAA+AGG + chr1.2:56966463-56966482 None:intergenic 30.0%
TAGCTTAAGGATATCTTTGA+AGG - chr1.2:56967744-56967763 MS.gene32145:CDS 30.0%
TCTCATATAGATATGAAGAG+AGG - chr1.2:56967328-56967347 MS.gene32145:CDS 30.0%
TCTTCTACTAGTAGAAAACA+TGG - chr1.2:56966476-56966495 MS.gene32145:CDS 30.0%
TTCATCATATTATGAGCAAG+CGG + chr1.2:56967868-56967887 None:intergenic 30.0%
! AATCTGAGATAGCAAAGTTA+AGG - chr1.2:56966603-56966622 MS.gene32145:CDS 30.0%
! AATTTGGTTTCTTCAGAAGA+GGG - chr1.2:56966656-56966675 MS.gene32145:CDS 30.0%
! CTTAGCTTTCCACATTTAAT+TGG - chr1.2:56967682-56967701 MS.gene32145:CDS 30.0%
! TTGGAATCAGACTTTTGATA+AGG - chr1.2:56967057-56967076 MS.gene32145:CDS 30.0%
AATGAGATCTTGAACTCAGA+GGG - chr1.2:56966626-56966645 MS.gene32145:CDS 35.0%
ACAACAACAAAACGCATTAC+TGG - chr1.2:56968038-56968057 MS.gene32145:CDS 35.0%
ACAACAGCTAATCCACTATT+TGG + chr1.2:56967583-56967602 None:intergenic 35.0%
AGCTTAAGGAGATTTCACTT+TGG - chr1.2:56967038-56967057 MS.gene32145:CDS 35.0%
AGCTTGATACACAACTGTAT+TGG + chr1.2:56967495-56967514 None:intergenic 35.0%
AGTTAAGCATATGGAAGGAA+TGG - chr1.2:56966847-56966866 MS.gene32145:CDS 35.0%
ATGAAATGGAAATTGCAGTG+CGG - chr1.2:56968093-56968112 MS.gene32145:CDS 35.0%
CAACAACAAAACGCATTACT+GGG - chr1.2:56968039-56968058 MS.gene32145:CDS 35.0%
CATACAGATTCTTGCATACA+AGG + chr1.2:56967113-56967132 None:intergenic 35.0%
CGCAATCATAGCAAGAAAAA+TGG - chr1.2:56967265-56967284 MS.gene32145:CDS 35.0%
CTTCACAATTACCAATCACA+AGG + chr1.2:56967165-56967184 None:intergenic 35.0%
CTTGTATGCAAGAATCTGTA+TGG - chr1.2:56967111-56967130 MS.gene32145:CDS 35.0%
GGTTACAATCTGAGTTCAAT+TGG - chr1.2:56967286-56967305 MS.gene32145:CDS 35.0%
GTAGAAAACATGGTCCTAAA+AGG - chr1.2:56966486-56966505 MS.gene32145:CDS 35.0%
TAATCCACTATTTGGACCAA+AGG + chr1.2:56967575-56967594 None:intergenic 35.0%
TCAGAATAATAGCCAGAATG+AGG - chr1.2:56966961-56966980 MS.gene32145:CDS 35.0%
TGCTGATAAGATAAAGACAG+AGG - chr1.2:56967966-56967985 MS.gene32145:CDS 35.0%
TTGTGCAAGCAGTTTCAATT+TGG - chr1.2:56968062-56968081 MS.gene32145:CDS 35.0%
! CTTGTGATTGGTAATTGTGA+AGG - chr1.2:56967163-56967182 MS.gene32145:CDS 35.0%
! GAATTTGGTTTCTTCAGAAG+AGG - chr1.2:56966655-56966674 MS.gene32145:CDS 35.0%
! GTCTAGCTTGATTAATGTTG+AGG - chr1.2:56967219-56967238 MS.gene32145:CDS 35.0%
! TGATAAGGTTGTTGAGTTGT+TGG - chr1.2:56967072-56967091 MS.gene32145:CDS 35.0%
!! GAAGCTTGGATTTTGAAGAT+GGG - chr1.2:56966407-56966426 MS.gene32145:CDS 35.0%
!! TGAAGCTTGGATTTTGAAGA+TGG - chr1.2:56966406-56966425 MS.gene32145:CDS 35.0%
!!! GAAGATTTTGGTTTTCCTTG+TGG - chr1.2:56967373-56967392 MS.gene32145:CDS 35.0%
!!! GGGGTTTTTAGTTAAGCATA+TGG - chr1.2:56966838-56966857 MS.gene32145:CDS 35.0%
CAAGCTCTCAAAGTCCTATT+GGG - chr1.2:56967509-56967528 MS.gene32145:CDS 40.0%
CTGAGTTCAATTGGGAGAAA+TGG - chr1.2:56967295-56967314 MS.gene32145:CDS 40.0%
CTTTCCACATTTAATTGGCG+AGG - chr1.2:56967687-56967706 MS.gene32145:CDS 40.0%
GAATGAGATCTTGAACTCAG+AGG - chr1.2:56966625-56966644 MS.gene32145:CDS 40.0%
GAATGGTGAGGAAAATGGAT+AGG - chr1.2:56966864-56966883 MS.gene32145:CDS 40.0%
GCAAGAATCTGTATGGTCTT+TGG - chr1.2:56967118-56967137 MS.gene32145:CDS 40.0%
TCAACCTTTGGTCCAAATAG+TGG - chr1.2:56967568-56967587 MS.gene32145:CDS 40.0%
TCAAGCTCTCAAAGTCCTAT+TGG - chr1.2:56967508-56967527 MS.gene32145:CDS 40.0%
TCTCAAAGTCCTATTGGGAT+TGG - chr1.2:56967514-56967533 MS.gene32145:CDS 40.0%
TGATGAAATGGCAAAGTGAG+AGG - chr1.2:56967881-56967900 MS.gene32145:CDS 40.0%
TGGGCAAAATGAATGTGGTT+CGG - chr1.2:56967195-56967214 MS.gene32145:CDS 40.0%
TTGGGAGAAATGGTAGTAGT+AGG - chr1.2:56967305-56967324 MS.gene32145:CDS 40.0%
! ATAGGTATGTGAGTGCTACT+AGG - chr1.2:56966882-56966901 MS.gene32145:CDS 40.0%
! GTGTATGGTGATGTTGTTAG+TGG - chr1.2:56966794-56966813 MS.gene32145:CDS 40.0%
AATGGGGGGTTTGAATGAGT+TGG - chr1.2:56966919-56966938 MS.gene32145:CDS 45.0%
AATTGTGAAGGTTCGCCACT+TGG - chr1.2:56967175-56967194 MS.gene32145:CDS 45.0%
AGAATGAGGAGAGTAGAAGG+GGG - chr1.2:56966975-56966994 MS.gene32145:CDS 45.0%
AGCATATGGAAGGAATGGTG+AGG - chr1.2:56966852-56966871 MS.gene32145:CDS 45.0%
ATTGTGAAGGTTCGCCACTT+GGG - chr1.2:56967176-56967195 MS.gene32145:CDS 45.0%
CAGAATGAGGAGAGTAGAAG+GGG - chr1.2:56966974-56966993 MS.gene32145:CDS 45.0%
CATTCCATGAAAAGTCTCCC+AGG + chr1.2:56967406-56967425 None:intergenic 45.0%
CCACTTGGGCAAAATGAATG+TGG - chr1.2:56967190-56967209 MS.gene32145:CDS 45.0%
CCAGAATGAGGAGAGTAGAA+GGG - chr1.2:56966973-56966992 MS.gene32145:CDS 45.0%
CGACTCTTGATGGGATACTT+CGG - chr1.2:56967833-56967852 MS.gene32145:CDS 45.0%
GAGCACTTATGCTCAACCTT+TGG - chr1.2:56967556-56967575 MS.gene32145:CDS 45.0%
GCTTCCTCGCCAATTAAATG+TGG + chr1.2:56967694-56967713 None:intergenic 45.0%
GGAAGGAATGGTGAGGAAAA+TGG - chr1.2:56966859-56966878 MS.gene32145:CDS 45.0%
TATGCTCCTCCTTCTACTCT+TGG - chr1.2:56967604-56967623 MS.gene32145:CDS 45.0%
TATGGCAGAAGCAAGATGTG+AGG - chr1.2:56967014-56967033 MS.gene32145:CDS 45.0%
TTCCTTGTGGAACAAGTCCT+GGG - chr1.2:56967386-56967405 MS.gene32145:CDS 45.0%
TTTCCTTGTGGAACAAGTCC+TGG - chr1.2:56967385-56967404 MS.gene32145:CDS 45.0%
! AAGTCCTGGGAGACTTTTCA+TGG - chr1.2:56967399-56967418 MS.gene32145:CDS 45.0%
! AGGTTGTTGAGTTGTTGGCT+AGG - chr1.2:56967077-56967096 MS.gene32145:CDS 45.0%
! CACAACTGTATTGGTCCTCA+CGG + chr1.2:56967486-56967505 None:intergenic 45.0%
! CCACATTCATTTTGCCCAAG+TGG + chr1.2:56967193-56967212 None:intergenic 45.0%
! CTGGTGTGGTTTCTAGGTTA+GGG - chr1.2:56966732-56966751 MS.gene32145:CDS 45.0%
! CTTTGGTGATTCTACGAGGA+AGG - chr1.2:56967135-56967154 MS.gene32145:CDS 45.0%
! GAGAGGAATTTTGAGCAACG+CGG - chr1.2:56967898-56967917 MS.gene32145:CDS 45.0%
! TGGTCTTTGGTGATTCTACG+AGG - chr1.2:56967131-56967150 MS.gene32145:CDS 45.0%
!! AGGAAGGATAGCCTTGTGAT+TGG - chr1.2:56967151-56967170 MS.gene32145:CDS 45.0%
!! CAAGGCTTTGAGCATGTGTA+TGG - chr1.2:56966779-56966798 MS.gene32145:CDS 45.0%
!! CTCAGAGGGTGTTAGGAATT+TGG - chr1.2:56966640-56966659 MS.gene32145:CDS 45.0%
!! TCTTGAACTCAGAGGGTGTT+AGG - chr1.2:56966633-56966652 MS.gene32145:CDS 45.0%
!! TGCAAGAGGGGCATCATATA+TGG + chr1.2:56967800-56967819 None:intergenic 45.0%
ACTGGAAGGCGACTCTTGAT+GGG - chr1.2:56967824-56967843 MS.gene32145:CDS 50.0%
AGCAACGCGGAATAGTTAGC+AGG - chr1.2:56967911-56967930 MS.gene32145:CDS 50.0%
CATCCTCCAAGAGTAGAAGG+AGG + chr1.2:56967613-56967632 None:intergenic 50.0%
CCCTTCTACTCTCCTCATTC+TGG + chr1.2:56966976-56966995 None:intergenic 50.0%
CCTTCCAGTCATGTGCAAGA+GGG + chr1.2:56967813-56967832 None:intergenic 50.0%
CTCTTGATGGGATACTTCGG+TGG - chr1.2:56967836-56967855 MS.gene32145:CDS 50.0%
CTTCCAGTCATGTGCAAGAG+GGG + chr1.2:56967812-56967831 None:intergenic 50.0%
GCAAGATGTGAGGCAGCTTA+AGG - chr1.2:56967024-56967043 MS.gene32145:CDS 50.0%
GCACATCCTCCAAGAGTAGA+AGG + chr1.2:56967616-56967635 None:intergenic 50.0%
GCCAGAATGAGGAGAGTAGA+AGG - chr1.2:56966972-56966991 MS.gene32145:CDS 50.0%
GGTTTGAGCAGAGGCTTGTA+TGG - chr1.2:56966996-56967015 MS.gene32145:CDS 50.0%
TAGAAACCACACCAGCAACG+CGG + chr1.2:56966727-56966746 None:intergenic 50.0%
TGATGTTGCTATCGACCGTG+AGG - chr1.2:56967468-56967487 MS.gene32145:CDS 50.0%
TGCTCAAAGCCTTGCAAAGC+AGG + chr1.2:56966773-56966792 None:intergenic 50.0%
TGTTCTGTGCCTGCTTTGCA+AGG - chr1.2:56966761-56966780 MS.gene32145:CDS 50.0%
! GCTGGTGTGGTTTCTAGGTT+AGG - chr1.2:56966731-56966750 MS.gene32145:CDS 50.0%
!! AGATGCTGCCGACTAGCTTA+AGG - chr1.2:56967731-56967750 MS.gene32145:CDS 50.0%
ATGCCCCTCTTGCACATGAC+TGG - chr1.2:56967806-56967825 MS.gene32145:CDS 55.0%
ATTGAACCGCGTTGCTGGTG+TGG - chr1.2:56966718-56966737 MS.gene32145:CDS 55.0%
CCCTCTTGCACATGACTGGA+AGG - chr1.2:56967810-56967829 MS.gene32145:CDS 55.0%
GACTGGAAGGCGACTCTTGA+TGG - chr1.2:56967823-56967842 MS.gene32145:CDS 55.0%
GAGGAATTGAACCGCGTTGC+TGG - chr1.2:56966713-56966732 MS.gene32145:CDS 55.0%
GCCTTCCAGTCATGTGCAAG+AGG + chr1.2:56967814-56967833 None:intergenic 55.0%
GCGTTGCTGGTGTGGTTTCT+AGG - chr1.2:56966726-56966745 MS.gene32145:CDS 55.0%
GCTCCTCCTTCTACTCTTGG+AGG - chr1.2:56967607-56967626 MS.gene32145:CDS 55.0%
GTAGAAGGGGGTTTGAGCAG+AGG - chr1.2:56966987-56967006 MS.gene32145:CDS 55.0%
TGTTCGACGTGAGAAGCTCG+AGG - chr1.2:56966694-56966713 MS.gene32145:CDS 55.0%
! CTCCCAGGACTTGTTCCACA+AGG + chr1.2:56967391-56967410 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 56966398 56968134 56966398 ID=MS.gene32145
chr1.2 mRNA 56966398 56968134 56966398 ID=MS.gene32145.t1;Parent=MS.gene32145
chr1.2 exon 56966398 56968134 56966398 ID=MS.gene32145.t1.exon1;Parent=MS.gene32145.t1
chr1.2 CDS 56966398 56968134 56966398 ID=cds.MS.gene32145.t1;Parent=MS.gene32145.t1
Gene Sequence

>MS.gene32145

ATGGTTGCTGAAGCTTGGATTTTGAAGATGGGTAACCAAGTAAGTGCAAATATTAAACAGGCCCTTTTGCTTGAAACTTCTTCTACTAGTAGAAAACATGGTCCTAAAAGGAAAGAGAATAAGAAAGTAGAAACTATAGGTATTCTTTCTTTTGAAGTAGCTAATGTAATGTCCAAAACCATTCAACTTTATAAATCATTGTCTGAATCTGAGATAGCAAAGTTAAGGAATGAGATCTTGAACTCAGAGGGTGTTAGGAATTTGGTTTCTTCAGAAGAGGGTTATTTATTCGAGCTTGTTCGACGTGAGAAGCTCGAGGAATTGAACCGCGTTGCTGGTGTGGTTTCTAGGTTAGGGAAGAAGTGTTCTGTGCCTGCTTTGCAAGGCTTTGAGCATGTGTATGGTGATGTTGTTAGTGGTGTTATAGATGTGAATGAATTGGGGTTTTTAGTTAAGCATATGGAAGGAATGGTGAGGAAAATGGATAGGTATGTGAGTGCTACTAGGAGTTTGTATAGTAAAATGGGGGGTTTGAATGAGTTGGAGCAAACACTGAAGAAGTTTCAGAATAATAGCCAGAATGAGGAGAGTAGAAGGGGGTTTGAGCAGAGGCTTGTATGGCAGAAGCAAGATGTGAGGCAGCTTAAGGAGATTTCACTTTGGAATCAGACTTTTGATAAGGTTGTTGAGTTGTTGGCTAGGACAGTTTGTACCTTGTATGCAAGAATCTGTATGGTCTTTGGTGATTCTACGAGGAAGGATAGCCTTGTGATTGGTAATTGTGAAGGTTCGCCACTTGGGCAAAATGAATGTGGTTCGGCGTCTAGCTTGATTAATGTTGAGGTGAATTTGTCGGAGAAGTTGAAACGCAATCATAGCAAGAAAAATGGTTACAATCTGAGTTCAATTGGGAGAAATGGTAGTAGTAGGTCTCATATAGATATGAAGAGAGGTGACTTAGCATATGTTCAACTTGAAGATTTTGGTTTTCCTTGTGGAACAAGTCCTGGGAGACTTTTCATGGAATGTCTCAGTTTAAGTAGCTCAGTTTCAAAATTCGACGATTTTGATGATGTTGCTATCGACCGTGAGGACCAATACAGTTGTGTATCAAGCTCTCAAAGTCCTATTGGGATTGGAAATATTGTCAAGAAAAAGGAGCACTTATGCTCAACCTTTGGTCCAAATAGTGGATTAGCTGTTGTTTATGCTCCTCCTTCTACTCTTGGAGGATGTGCTCTAGCATTGCATTATGCTAATATCATTATTGTCATTGAGAAAGTACTTAGCTTTCCACATTTAATTGGCGAGGAAGCTAGAGATGATCTATATAAGATGCTGCCGACTAGCTTAAGGATATCTTTGAAGGCCAAACTTAAGACATATGTCAAGAATTTGGCCATATATGATGCCCCTCTTGCACATGACTGGAAGGCGACTCTTGATGGGATACTTCGGTGGCTTGCTCCGCTTGCTCATAATATGATGAAATGGCAAAGTGAGAGGAATTTTGAGCAACGCGGAATAGTTAGCAGGACAAATGTGCTACTATTTCAGACATTATACTTTGCTGATAAGATAAAGACAGAGGAAGCTATATGTGAACTTCTTAAAGGATTGAACTATATATGTCGTTATGAACAACAACAAAACGCATTACTGGGTTGTGCAAGCAGTTTCAATTTGGAAGATTGCATGAAATGGAAATTGCAGTGCGGTGATTCGTTACTCGATTAA
Protein sequence

>MS.gene32145.t1

MVAEAWILKMGNQVSANIKQALLLETSSTSRKHGPKRKENKKVETIGILSFEVANVMSKTIQLYKSLSESEIAKLRNEILNSEGVRNLVSSEEGYLFELVRREKLEELNRVAGVVSRLGKKCSVPALQGFEHVYGDVVSGVIDVNELGFLVKHMEGMVRKMDRYVSATRSLYSKMGGLNELEQTLKKFQNNSQNEESRRGFEQRLVWQKQDVRQLKEISLWNQTFDKVVELLARTVCTLYARICMVFGDSTRKDSLVIGNCEGSPLGQNECGSASSLINVEVNLSEKLKRNHSKKNGYNLSSIGRNGSSRSHIDMKRGDLAYVQLEDFGFPCGTSPGRLFMECLSLSSSVSKFDDFDDVAIDREDQYSCVSSSQSPIGIGNIVKKKEHLCSTFGPNSGLAVVYAPPSTLGGCALALHYANIIIVIEKVLSFPHLIGEEARDDLYKMLPTSLRISLKAKLKTYVKNLAIYDAPLAHDWKATLDGILRWLAPLAHNMMKWQSERNFEQRGIVSRTNVLLFQTLYFADKIKTEEAICELLKGLNYICRYEQQQNALLGCASSFNLEDCMKWKLQCGDSLLD