AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32259

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32259.t1 MTR_0034s0060 99.401 668 4 0 1 668 131 798 0.0 1386
MS.gene32259.t1 MTR_2g073240 89.671 668 68 1 1 667 13 680 0.0 1268
MS.gene32259.t1 MTR_1g009600 73.835 665 161 3 4 667 2 654 0.0 1036
MS.gene32259.t1 MTR_4g102550 83.509 285 47 0 383 667 11 295 4.79e-178 508
MS.gene32259.t1 MTR_3g452330 51.381 181 36 1 85 265 1 129 2.62e-48 170
MS.gene32259.t1 MTR_3g452330 60.000 100 40 0 375 474 138 237 8.25e-34 130
MS.gene32259.t1 MTR_8g089180 26.000 350 218 13 8 327 72 410 5.04e-25 108
MS.gene32259.t1 MTR_8g089180 26.000 350 218 13 8 327 72 410 5.04e-25 108
MS.gene32259.t1 MTR_4g063600 27.974 311 201 10 9 312 34 328 5.28e-25 107
MS.gene32259.t1 MTR_2g096660 29.618 314 192 10 9 312 40 334 5.34e-25 107
MS.gene32259.t1 MTR_4g120030 26.979 341 216 12 1 332 105 421 8.01e-25 108
MS.gene32259.t1 MTR_4g063600 27.653 311 199 11 9 312 34 325 2.07e-24 105
MS.gene32259.t1 MTR_2g032100 25.788 349 220 12 8 327 72 410 5.21e-24 105
MS.gene32259.t1 MTR_1g088480 27.653 311 196 12 9 312 120 408 3.43e-23 103
MS.gene32259.t1 MTR_4g129820 28.190 337 207 10 8 319 96 422 1.60e-22 101
MS.gene32259.t1 MTR_4g024600 27.545 334 213 8 8 319 102 428 1.80e-21 98.2
MS.gene32259.t1 MTR_5g009170 27.066 351 215 14 7 327 47 386 3.73e-20 93.6
MS.gene32259.t1 MTR_0354s0040 27.405 343 217 9 2 319 74 409 1.45e-19 92.0
MS.gene32259.t1 MTR_8g099535 27.557 352 214 13 7 328 4 344 2.30e-19 90.5
MS.gene32259.t1 MTR_4g102270 26.724 348 208 10 3 319 89 420 6.64e-19 90.1
MS.gene32259.t1 MTR_5g011940 26.136 352 225 10 3 326 86 430 9.10e-19 89.7
MS.gene32259.t1 MTR_3g084090 26.866 335 216 10 9 321 101 428 1.91e-18 89.0
MS.gene32259.t1 MTR_2g032100 28.272 191 124 5 8 193 72 254 1.57e-16 81.3
MS.gene32259.t1 MTR_4g134490 23.429 350 229 13 7 327 25 364 2.14e-16 82.0
MS.gene32259.t1 MTR_4g134490 23.315 356 228 14 7 327 25 370 1.06e-14 76.6
MS.gene32259.t1 MTR_3g105440 30.242 248 142 10 1 234 1 231 2.07e-14 77.0
MS.gene32259.t1 MTR_3g105440 29.388 245 148 9 1 234 1 231 2.07e-14 77.0
MS.gene32259.t1 MTR_7g108490 27.083 192 124 6 141 322 131 316 9.26e-12 67.0
MS.gene32259.t1 MTR_7g108490 27.083 192 124 6 141 322 132 317 9.52e-12 67.0
MS.gene32259.t1 MTR_5g009160 27.473 273 163 12 81 327 17 280 3.11e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32259.t1 AT1G78570 88.209 670 74 2 1 667 1 668 0.0 1243
MS.gene32259.t1 AT3G14790 85.757 667 91 2 1 667 4 666 0.0 1169
MS.gene32259.t1 AT3G14790 85.757 667 91 2 1 667 1 663 0.0 1168
MS.gene32259.t1 AT1G53500 84.535 666 100 2 2 667 4 666 0.0 1160
MS.gene32259.t1 AT1G63000 81.690 284 52 0 384 667 13 296 6.81e-178 508
MS.gene32259.t1 AT4G20460 27.401 354 208 13 8 327 71 409 3.81e-28 117
MS.gene32259.t1 AT2G28760 29.073 313 195 10 9 312 33 327 2.98e-26 110
MS.gene32259.t1 AT2G28760 29.073 313 195 10 9 312 33 327 2.98e-26 110
MS.gene32259.t1 AT2G28760 29.073 313 195 10 9 312 33 327 2.98e-26 110
MS.gene32259.t1 AT2G28760 29.073 313 195 10 9 312 64 358 6.02e-26 110
MS.gene32259.t1 AT5G59290 28.754 313 196 10 9 312 3 297 8.88e-26 108
MS.gene32259.t1 AT5G59290 28.754 313 196 10 9 312 32 326 1.16e-25 109
MS.gene32259.t1 AT5G59290 28.754 313 196 10 9 312 25 319 1.35e-25 108
MS.gene32259.t1 AT3G62830 27.796 313 198 10 9 312 121 414 1.39e-25 110
MS.gene32259.t1 AT3G62830 27.796 313 198 10 9 312 121 414 1.39e-25 110
MS.gene32259.t1 AT5G59290 28.754 313 196 10 9 312 47 341 1.56e-25 108
MS.gene32259.t1 AT3G46440 28.435 313 197 10 9 312 31 325 3.87e-25 107
MS.gene32259.t1 AT3G46440 28.435 313 197 10 9 312 31 325 3.87e-25 107
MS.gene32259.t1 AT3G53520 27.302 315 205 10 9 316 122 419 6.41e-25 108
MS.gene32259.t1 AT2G47650 27.157 313 200 10 9 312 123 416 3.26e-24 106
MS.gene32259.t1 AT5G44480 26.934 349 214 14 8 326 72 409 8.41e-24 105
MS.gene32259.t1 AT5G44480 26.934 349 214 14 8 326 72 409 8.41e-24 105
MS.gene32259.t1 AT5G44480 26.934 349 214 14 8 326 72 409 8.41e-24 105
MS.gene32259.t1 AT5G44480 26.934 349 214 14 8 326 96 433 1.01e-23 105
MS.gene32259.t1 AT1G30620 27.714 350 212 14 8 327 71 409 2.34e-23 103
MS.gene32259.t1 AT1G30620 27.714 350 212 14 8 327 72 410 2.72e-23 103
MS.gene32259.t1 AT1G30620 27.714 350 212 14 8 327 72 410 2.72e-23 103
MS.gene32259.t1 AT1G30620 27.714 350 212 14 8 327 72 410 2.72e-23 103
MS.gene32259.t1 AT1G30620 27.714 350 212 14 8 327 72 410 2.72e-23 103
MS.gene32259.t1 AT2G47650 26.814 317 202 10 9 312 123 422 4.06e-23 103
MS.gene32259.t1 AT3G53520 26.984 315 204 11 9 316 122 417 1.38e-22 101
MS.gene32259.t1 AT3G53520 26.331 338 202 11 9 316 122 442 1.54e-22 101
MS.gene32259.t1 AT2G45310 27.628 333 212 9 9 319 99 424 2.24e-21 97.8
MS.gene32259.t1 AT1G63180 26.611 357 221 13 1 327 1 346 8.88e-21 94.7
MS.gene32259.t1 AT4G00110 27.246 334 214 8 8 319 92 418 1.88e-20 95.1
MS.gene32259.t1 AT1G02000 26.647 334 216 9 8 319 93 419 1.09e-19 92.8
MS.gene32259.t1 AT1G30620 30.366 191 120 5 8 193 72 254 1.36e-18 87.4
MS.gene32259.t1 AT4G30440 30.043 233 153 5 8 234 89 317 3.37e-18 88.2
MS.gene32259.t1 AT3G53520 27.897 233 148 7 8 234 121 339 1.79e-17 85.1
MS.gene32259.t1 AT3G23820 26.048 334 218 9 8 319 113 439 2.53e-16 82.4
MS.gene32259.t1 AT1G64440 27.066 351 213 15 8 327 4 342 4.71e-16 80.5
MS.gene32259.t1 AT4G12250 26.048 334 218 8 8 319 97 423 3.49e-15 79.0
MS.gene32259.t1 AT1G63180 27.365 296 169 12 1 262 1 284 3.62e-15 77.4
MS.gene32259.t1 AT4G23920 25.291 344 222 12 7 323 3 338 1.57e-14 75.9
MS.gene32259.t1 AT1G47290 29.918 244 140 11 5 234 8 234 2.96e-13 72.4
MS.gene32259.t1 AT1G47290 29.918 244 140 11 5 234 8 234 8.36e-13 71.6
MS.gene32259.t1 AT2G26260 26.104 249 149 8 2 234 5 234 1.00e-12 71.6
MS.gene32259.t1 AT1G73250 26.923 182 121 5 151 322 140 319 5.41e-12 68.2

Find 174 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGGAATTCTTTCTTTAATT+TGG 0.120604 8.3:+48634415 None:intergenic
ACACGTGGAAACAATGTTTA+TGG 0.186415 8.3:-48635876 MS.gene32259:CDS
TCTTTGATGGAAAGCAATTC+TGG 0.218557 8.3:+48634395 None:intergenic
ATAGAAAGATCCCATGAAAT+TGG 0.250976 8.3:+48634903 None:intergenic
AAACCAATGCCGGAGCCTTC+TGG 0.251282 8.3:+48634943 None:intergenic
CTGCGGAGTGGCAGTGTTCT+TGG 0.257055 8.3:+48635308 None:intergenic
TCTGTTTATTTCTTCTTGTT+TGG 0.260972 8.3:+48634347 None:intergenic
TTAGAAGCCTGCAAGGTTAC+TGG 0.269609 8.3:-48636083 MS.gene32259:CDS
CTTGATAAGTGATTCTTTGA+TGG 0.279707 8.3:+48634382 None:intergenic
GAAATTCTGCCACTTGAAAC+TGG 0.287832 8.3:+48634499 None:intergenic
TTTGCCATCTCGATTGAAAT+AGG 0.290998 8.3:+48634611 None:intergenic
AGGGTGGATTGGAGGTTTGC+TGG 0.296271 8.3:-48635250 MS.gene32259:CDS
AACTTCTCGGGAAACTGATT+TGG 0.320333 8.3:+48635852 None:intergenic
TTTCCACGTGTTGTGATAAC+AGG 0.321129 8.3:+48635888 None:intergenic
ACTTACCATAGCTTTAGTTT+TGG 0.328658 8.3:+48634879 None:intergenic
GTCAGTACCGATGAGGTCTA+TGG 0.334959 8.3:-48636038 MS.gene32259:CDS
ACAAATTTAATGTTAGCTTC+TGG 0.336048 8.3:+48635609 None:intergenic
GATTCCAAAGTTCATCCTTT+TGG 0.336822 8.3:-48635829 MS.gene32259:CDS
AGTTCCATATATGTTGTTCT+TGG 0.337491 8.3:+48636115 None:intergenic
GTTGGCCATGTTTACAATAT+CGG 0.337863 8.3:-48635696 MS.gene32259:CDS
ATGAGATCCTTGAGATGTAT+AGG 0.343554 8.3:-48634535 MS.gene32259:CDS
TCCAATACTGTCATGCTGTT+TGG 0.344679 8.3:+48634644 None:intergenic
TTCTTGCTGTTTGGTAGGAC+AGG 0.352546 8.3:-48635270 MS.gene32259:CDS
GATCTGGCCAGTAACCTTGC+AGG 0.353065 8.3:+48636076 None:intergenic
TTCACCAAGAACAACATATA+TGG 0.354265 8.3:-48636119 MS.gene32259:CDS
CCAATCAACATTGGGTCTTC+CGG 0.367734 8.3:+48635101 None:intergenic
CCTTTGAAGTTCTTGCTGTT+TGG 0.369703 8.3:-48635279 MS.gene32259:CDS
CGACCCTTTGCATATTCATA+TGG 0.380468 8.3:+48635201 None:intergenic
TGTCAACTACTTGCTTATTA+CGG 0.386916 8.3:-48636213 MS.gene32259:CDS
GGTAGGACAGGGTGGATTGG+AGG 0.391913 8.3:-48635258 MS.gene32259:CDS
AGAGAAACTTGAGTGGAATC+TGG 0.393435 8.3:-48634589 MS.gene32259:CDS
GTGGCAGTGTTCTTGGTTGA+TGG 0.400754 8.3:+48635315 None:intergenic
CTTTGATGGAAAGCAATTCT+GGG 0.407319 8.3:+48634396 None:intergenic
CCCCTTCACAAACTTAAAGT+TGG 0.408501 8.3:+48636256 None:intergenic
GGAAGACAAGCCCAATTTCA+TGG 0.408529 8.3:-48634914 MS.gene32259:CDS
ACCGAAGAATATCCTCATTA+CGG 0.409644 8.3:-48636411 MS.gene32259:CDS
ACCATCCGCGCTAATGTTGC+TGG 0.416077 8.3:-48635057 MS.gene32259:CDS
GGAAAGACTCTTCCCATTCA+TGG 0.417261 8.3:-48635798 MS.gene32259:CDS
ATGACAATGTATGCACCCTT+AGG 0.418171 8.3:-48634751 MS.gene32259:CDS
CCCTTCACAAACTTAAAGTT+GGG 0.422846 8.3:+48636257 None:intergenic
AGGCTCCGGCATTGGTTTCA+AGG 0.423913 8.3:-48634938 MS.gene32259:CDS
TGTTGCAGAGTACGGGTTTG+TGG 0.424954 8.3:+48635962 None:intergenic
ACTCATGTCGACAACTCATT+TGG 0.427673 8.3:-48636155 MS.gene32259:CDS
CCGAAGAATATCCTCATTAC+GGG 0.434381 8.3:-48636410 MS.gene32259:CDS
TTTGGTAGGACAGGGTGGAT+TGG 0.439952 8.3:-48635261 MS.gene32259:CDS
TTGGCCATGTTTACAATATC+GGG 0.445880 8.3:-48635695 MS.gene32259:CDS
AACTTTGGAATCAACTTCTC+GGG 0.447682 8.3:+48635840 None:intergenic
TGAGATCCTTGAGATGTATA+GGG 0.450832 8.3:-48634534 MS.gene32259:CDS
GAACTTTGGAATCAACTTCT+CGG 0.452334 8.3:+48635839 None:intergenic
TAGAAAGATCCCATGAAATT+GGG 0.452975 8.3:+48634904 None:intergenic
TTGCATCTCATGTTGCCAAC+CGG 0.455327 8.3:-48636373 MS.gene32259:CDS
CACGTGGAAACAATGTTTAT+GGG 0.456078 8.3:-48635875 MS.gene32259:CDS
ATTAGAACCATCCCCATGAA+TGG 0.458484 8.3:+48635785 None:intergenic
ACCGATGAAGATGCTATTGT+TGG 0.459550 8.3:-48636011 MS.gene32259:CDS
GAAATGCTTGTCATGGCATA+TGG 0.461925 8.3:-48635927 MS.gene32259:CDS
CATCCAGAAGGCTCCGGCAT+TGG 0.462462 8.3:-48634946 MS.gene32259:CDS
AAGTATCTCTGGTCATTAAA+CGG 0.464062 8.3:+48635576 None:intergenic
TCATTGTGGCTCACAGCACC+AGG 0.470389 8.3:+48634554 None:intergenic
CCGGAAGACCCAATGTTGAT+TGG 0.478270 8.3:-48635101 MS.gene32259:CDS
ATGGCATATGGTAGGTCATA+CGG 0.483908 8.3:-48635915 MS.gene32259:CDS
GGGTGGATTGGAGGTTTGCT+GGG 0.485086 8.3:-48635249 MS.gene32259:CDS
AAATTCTGCCACTTGAAACT+GGG 0.485944 8.3:+48634500 None:intergenic
TGGCAGTGTTCTTGGTTGAT+GGG 0.488497 8.3:+48635316 None:intergenic
CATGAATGAACCTTCTGATC+TGG 0.488900 8.3:+48636060 None:intergenic
GTTGCAGAGTACGGGTTTGT+GGG 0.489248 8.3:+48635963 None:intergenic
TTGCTCAGATCAGATGAAAT+CGG 0.490308 8.3:+48634719 None:intergenic
TGTTGCTGGCACTTTAACAT+TGG 0.496616 8.3:-48635043 MS.gene32259:CDS
CCCGTAATGAGGATATTCTT+CGG 0.496823 8.3:+48636410 None:intergenic
GATCATCGAGAAAGTATCTC+TGG 0.496831 8.3:+48635565 None:intergenic
TTAGATGAATTGACTGATGC+GGG 0.498621 8.3:+48635354 None:intergenic
TTGATGATAAATTATGCTAC+TGG 0.499263 8.3:-48634994 MS.gene32259:CDS
TGAAGTTCTTGCTGTTTGGT+AGG 0.503504 8.3:-48635275 MS.gene32259:CDS
TGGGAGATGATTTCGAAGGA+AGG 0.504770 8.3:+48636276 None:intergenic
GCCAGCAACATTAGCGCGGA+TGG 0.505695 8.3:+48635056 None:intergenic
GAAAAGCCTGCATATATCCT+TGG 0.507143 8.3:+48635641 None:intergenic
TCTTTCCTTGCATAGCCAAA+AGG 0.507278 8.3:+48635814 None:intergenic
GAAAGACTCTTCCCATTCAT+GGG 0.507489 8.3:-48635797 MS.gene32259:CDS
GTTGATGATGCCTGGTGGAA+TGG 0.508097 8.3:-48635409 MS.gene32259:CDS
TGACAATGTATGCACCCTTA+GGG 0.510852 8.3:-48634750 MS.gene32259:CDS
TCCAAACAGCATGACAGTAT+TGG 0.513133 8.3:-48634645 MS.gene32259:CDS
GTTTCGCACCAATCAACATT+GGG 0.513420 8.3:+48635093 None:intergenic
CTATTCCAAAACTAAAGCTA+TGG 0.514463 8.3:-48634884 MS.gene32259:intron
CCCAACTTTAAGTTTGTGAA+GGG 0.514912 8.3:-48636257 MS.gene32259:CDS
TCAGTACCGATGAGGTCTAT+GGG 0.515538 8.3:-48636037 MS.gene32259:CDS
ATAGCCAAAAGGATGAACTT+TGG 0.521511 8.3:+48635825 None:intergenic
GTTCTTCACAAGGGAGAGGT+TGG 0.523496 8.3:-48635714 MS.gene32259:CDS
GAGAGGACCACATGGGATGA+AGG 0.523892 8.3:-48635516 MS.gene32259:CDS
TTTAATGCTGCAGGTGTCAC+CGG 0.525836 8.3:-48635120 MS.gene32259:CDS
TGCTGTTTGGTAGGACAGGG+TGG 0.535213 8.3:-48635266 MS.gene32259:CDS
TGGCATATGGTAGGTCATAC+GGG 0.535706 8.3:-48635914 MS.gene32259:CDS
CCGAGGATGTTGATGATGCC+TGG 0.537047 8.3:-48635417 MS.gene32259:CDS
ATTCCATATGAATATGCAAA+GGG 0.537597 8.3:-48635204 MS.gene32259:CDS
TCCCAACTTTAAGTTTGTGA+AGG 0.538100 8.3:-48636258 MS.gene32259:CDS
ATCATCAACATCCTCGGATG+AGG 0.538319 8.3:+48635423 None:intergenic
CCAAACAGCAAGAACTTCAA+AGG 0.539699 8.3:+48635279 None:intergenic
CCGTACTCTGCAACAAAAGC+AGG 0.539993 8.3:-48635954 MS.gene32259:CDS
GCAGCACATCCAGAAGGCTC+CGG 0.540124 8.3:-48634952 MS.gene32259:CDS
TTCCTCCTTGAAACCAATGC+CGG 0.540843 8.3:+48634933 None:intergenic
TTAGAACCATCCCCATGAAT+GGG 0.541388 8.3:+48635786 None:intergenic
ACTCTTCCCATTCATGGGGA+TGG 0.542729 8.3:-48635792 MS.gene32259:CDS
CAACTTTAAGTTTGTGAAGG+GGG 0.542729 8.3:-48636255 MS.gene32259:CDS
TCGAGCAGCGACAATTACCT+TGG 0.547636 8.3:+48634460 None:intergenic
TCTTGCTGTTTGGTAGGACA+GGG 0.548224 8.3:-48635269 MS.gene32259:CDS
AGTTTCGCACCAATCAACAT+TGG 0.552063 8.3:+48635092 None:intergenic
TGTAATCCCTATACATCTCA+AGG 0.552164 8.3:+48634528 None:intergenic
CAATTCATCTAATACTCGTA+TGG 0.556213 8.3:-48635343 MS.gene32259:CDS
GACTGAAGAAAACCATAGAA+TGG 0.557484 8.3:-48635494 MS.gene32259:CDS
CGGGATGGCTTCGGACAAGC+CGG 0.558368 8.3:+48636354 None:intergenic
TTGTCCCGATATTGTAAACA+TGG 0.558984 8.3:+48635691 None:intergenic
AAATCGGCATACGAACCCTA+AGG 0.561199 8.3:+48634735 None:intergenic
GAAGACAAGCCCAATTTCAT+GGG 0.561539 8.3:-48634913 MS.gene32259:CDS
AGGTTACTGGCCAGATCAGA+AGG 0.563011 8.3:-48636070 MS.gene32259:CDS
TATCGGGACAAAGAAGGAAA+GGG 0.566922 8.3:-48635679 MS.gene32259:CDS
TTACAATATCGGGACAAAGA+AGG 0.567443 8.3:-48635685 MS.gene32259:CDS
TCTACCTATTTCAATCGAGA+TGG 0.567753 8.3:-48634615 MS.gene32259:CDS
ATCTGGAACTTCACTAACCC+TGG 0.570382 8.3:-48634572 MS.gene32259:CDS
AGTTGTTCTTCACAAGGGAG+AGG 0.570906 8.3:-48635718 MS.gene32259:CDS
CTTCAGTCCTTCATCCCATG+TGG 0.572232 8.3:+48635509 None:intergenic
CTCTGCAACAAAAGCAGGAG+CGG 0.574561 8.3:-48635949 MS.gene32259:CDS
ATATCGGGACAAAGAAGGAA+AGG 0.579866 8.3:-48635680 MS.gene32259:CDS
TCCAACAATAGCATCTTCAT+CGG 0.581847 8.3:+48636010 None:intergenic
CCAGGCATCATCAACATCCT+CGG 0.585840 8.3:+48635417 None:intergenic
ACGTAGCCAAGGATATATGC+AGG 0.587690 8.3:-48635647 MS.gene32259:CDS
GAAGAAGTTGAGAAGCCTCA+TGG 0.594333 8.3:+48635985 None:intergenic
CAAAGGATGTTTCTGCGGAG+TGG 0.596102 8.3:+48635296 None:intergenic
AACTTCAAAGGATGTTTCTG+CGG 0.601532 8.3:+48635291 None:intergenic
ATTAGATGAATTGACTGATG+CGG 0.603499 8.3:+48635353 None:intergenic
CACGCTTGAAGAGCAAGCCA+AGG 0.607735 8.3:-48634477 MS.gene32259:CDS
TGCTTGTCATGGCATATGGT+AGG 0.608244 8.3:-48635923 MS.gene32259:CDS
TGCTCGAAGCAACAATGAGA+TGG 0.612444 8.3:-48634444 MS.gene32259:CDS
TGTCTTATTAGAAGCCTGCA+AGG 0.613132 8.3:-48636090 MS.gene32259:CDS
GCTGATGTGTGCAGAGAGCA+CGG 0.615024 8.3:-48635021 MS.gene32259:CDS
ATTTGAAGTTGTTCTTCACA+AGG 0.615527 8.3:-48635724 MS.gene32259:CDS
AAATGTCTCTCCATTCCACC+AGG 0.615800 8.3:+48635399 None:intergenic
AGGAGCGGAAATGCTTGTCA+TGG 0.617019 8.3:-48635934 MS.gene32259:CDS
AAAACCTCGGTTGGGCTGAG+AGG 0.617287 8.3:-48635533 MS.gene32259:CDS
GGGAGTTATTGACGTAGCCA+AGG 0.619015 8.3:-48635658 MS.gene32259:CDS
GGTGGATTGGAGGTTTGCTG+GGG 0.620082 8.3:-48635248 MS.gene32259:CDS
TACGATGCAGCACATCCAGA+AGG 0.620237 8.3:-48634958 MS.gene32259:CDS
GGGAAATTGTGTGAAAAGCA+AGG 0.621950 8.3:-48635228 MS.gene32259:CDS
GTGCATTGCTTCCTCATCCG+AGG 0.623034 8.3:-48635434 MS.gene32259:CDS
ACATTGACCCCAGTTTCAAG+TGG 0.625778 8.3:-48634508 MS.gene32259:CDS
GTTGGGCTGAGAGGACCACA+TGG 0.627899 8.3:-48635524 MS.gene32259:CDS
ATGGCTTCGGACAAGCCGGT+TGG 0.627965 8.3:+48636358 None:intergenic
TTCATCTAATACTCGTATGG+TGG 0.628057 8.3:-48635340 MS.gene32259:CDS
ATGGCAAAGAGAAACTTGAG+TGG 0.635436 8.3:-48634596 MS.gene32259:CDS
ATCTTTGTGATGAAGTTGCG+TGG 0.638823 8.3:+48634695 None:intergenic
CCAACTTTAAGTTTGTGAAG+GGG 0.639389 8.3:-48636256 MS.gene32259:CDS
AATCGGCATACGAACCCTAA+GGG 0.640550 8.3:+48634736 None:intergenic
CGAAGAATATCCTCATTACG+GGG 0.642246 8.3:-48636409 MS.gene32259:CDS
TTTGAAGTTGTTCTTCACAA+GGG 0.643778 8.3:-48635723 MS.gene32259:CDS
ATGGGGATGGTTCTAATGTG+AGG 0.645313 8.3:-48635779 MS.gene32259:CDS
CGAAGCAACAATGAGATGGA+TGG 0.645825 8.3:-48634440 MS.gene32259:CDS
AATTCCATATGAATATGCAA+AGG 0.647655 8.3:-48635205 MS.gene32259:CDS
TTACCTGTTATCACAACACG+TGG 0.649186 8.3:-48635891 MS.gene32259:CDS
TGGTCCTCTCAGCCCAACCG+AGG 0.649775 8.3:+48635529 None:intergenic
CTGATGTGTGCAGAGAGCAC+GGG 0.653789 8.3:-48635020 MS.gene32259:CDS
ATCGGGACAAAGAAGGAAAG+GGG 0.655672 8.3:-48635678 MS.gene32259:CDS
TCTTTGTGATGAAGTTGCGT+GGG 0.656677 8.3:+48634696 None:intergenic
AATTCTGCCACTTGAAACTG+GGG 0.659291 8.3:+48634501 None:intergenic
GGTCTCCCCATAGACCTCAT+CGG 0.659778 8.3:+48636031 None:intergenic
TGCTATTGTTGGAAACCATG+AGG 0.663909 8.3:-48636000 MS.gene32259:CDS
ACATCTCAAGGATCTCATTG+TGG 0.665387 8.3:+48634540 None:intergenic
AAAGACTCTTCCCATTCATG+GGG 0.676326 8.3:-48635796 MS.gene32259:CDS
GTATTGTGAAGATGTTGCTG+AGG 0.681547 8.3:-48635748 MS.gene32259:CDS
CATTCATGTCAGTACCGATG+AGG 0.691762 8.3:-48636045 MS.gene32259:CDS
CAGTACCGATGAGGTCTATG+GGG 0.693201 8.3:-48636036 MS.gene32259:CDS
AAGTTGGGAGATGATTTCGA+AGG 0.693891 8.3:+48636272 None:intergenic
CTCCGGCATTGGTTTCAAGG+AGG 0.696239 8.3:-48634935 MS.gene32259:CDS
AAGTGCCAGCAACATTAGCG+CGG 0.700659 8.3:+48635052 None:intergenic
CATTGTGGCTCACAGCACCA+GGG 0.711227 8.3:+48634555 None:intergenic
TTGGGCTGAGAGGACCACAT+GGG 0.711320 8.3:-48635523 MS.gene32259:CDS
AGGATGTTGATGATGCCTGG+TGG 0.713184 8.3:-48635414 MS.gene32259:CDS
GAAGAATATCCTCATTACGG+GGG 0.731502 8.3:-48636408 MS.gene32259:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAGATCGTTCTATTTA+AGG + chr8.3:48635926-48635945 None:intergenic 20.0%
!! GAAAAAAATTCATAATGCAA+AGG + chr8.3:48635953-48635972 None:intergenic 20.0%
! AATTCCATATGAATATGCAA+AGG - chr8.3:48635572-48635591 MS.gene32259:CDS 25.0%
! ATTCCATATGAATATGCAAA+GGG - chr8.3:48635573-48635592 MS.gene32259:CDS 25.0%
! TGGGAATTCTTTCTTTAATT+TGG + chr8.3:48636365-48636384 None:intergenic 25.0%
!! ACAAATTTAATGTTAGCTTC+TGG + chr8.3:48635171-48635190 None:intergenic 25.0%
!! TTGATGATAAATTATGCTAC+TGG - chr8.3:48635783-48635802 MS.gene32259:CDS 25.0%
!!! ATTTTTGGTATACCATTCTA+TGG + chr8.3:48635298-48635317 None:intergenic 25.0%
!!! CTCTTAATTGAATTTTGATC+AGG - chr8.3:48635982-48636001 MS.gene32259:CDS 25.0%
AAGTATCTCTGGTCATTAAA+CGG + chr8.3:48635204-48635223 None:intergenic 30.0%
AGTTCCATATATGTTGTTCT+TGG + chr8.3:48634665-48634684 None:intergenic 30.0%
ATAGAAAGATCCCATGAAAT+TGG + chr8.3:48635877-48635896 None:intergenic 30.0%
ATTAGATGAATTGACTGATG+CGG + chr8.3:48635427-48635446 None:intergenic 30.0%
CAATTCATCTAATACTCGTA+TGG - chr8.3:48635434-48635453 MS.gene32259:CDS 30.0%
CTATTCCAAAACTAAAGCTA+TGG - chr8.3:48635893-48635912 MS.gene32259:CDS 30.0%
CTTGATAAGTGATTCTTTGA+TGG + chr8.3:48636398-48636417 None:intergenic 30.0%
TAGAAAGATCCCATGAAATT+GGG + chr8.3:48635876-48635895 None:intergenic 30.0%
TGTCAACTACTTGCTTATTA+CGG - chr8.3:48634564-48634583 MS.gene32259:CDS 30.0%
TTCACCAAGAACAACATATA+TGG - chr8.3:48634658-48634677 MS.gene32259:CDS 30.0%
! AAGCACAACAATTTTGTACT+CGG + chr8.3:48634446-48634465 None:intergenic 30.0%
! ATTTGAAGTTGTTCTTCACA+AGG - chr8.3:48635053-48635072 MS.gene32259:CDS 30.0%
! TTTGAAGTTGTTCTTCACAA+GGG - chr8.3:48635054-48635073 MS.gene32259:CDS 30.0%
!! ATTGAATTTTGATCAGGTTG+AGG - chr8.3:48635988-48636007 MS.gene32259:CDS 30.0%
!!! ACTTACCATAGCTTTAGTTT+TGG + chr8.3:48635901-48635920 None:intergenic 30.0%
ACACGTGGAAACAATGTTTA+TGG - chr8.3:48634901-48634920 MS.gene32259:CDS 35.0%
ACCGAAGAATATCCTCATTA+CGG - chr8.3:48634366-48634385 MS.gene32259:CDS 35.0%
ACCTGCAGCATTAAAAACAT+GGG + chr8.3:48635652-48635671 None:intergenic 35.0%
ATACCAAAAATCCTGATTGG+TGG - chr8.3:48635307-48635326 MS.gene32259:CDS 35.0%
ATAGCCAAAAGGATGAACTT+TGG + chr8.3:48634955-48634974 None:intergenic 35.0%
ATGAGATCCTTGAGATGTAT+AGG - chr8.3:48636242-48636261 MS.gene32259:CDS 35.0%
CACGTGGAAACAATGTTTAT+GGG - chr8.3:48634902-48634921 MS.gene32259:CDS 35.0%
CCCTTCACAAACTTAAAGTT+GGG + chr8.3:48634523-48634542 None:intergenic 35.0%
CTTTGATGGAAAGCAATTCT+GGG + chr8.3:48636384-48636403 None:intergenic 35.0%
GACTGAAGAAAACCATAGAA+TGG - chr8.3:48635283-48635302 MS.gene32259:CDS 35.0%
GGTATACCAAAAATCCTGAT+TGG - chr8.3:48635304-48635323 MS.gene32259:CDS 35.0%
GTTGGCCATGTTTACAATAT+CGG - chr8.3:48635081-48635100 MS.gene32259:CDS 35.0%
TACCAAAAATCCTGATTGGT+GGG - chr8.3:48635308-48635327 MS.gene32259:CDS 35.0%
TCCAACAATAGCATCTTCAT+CGG + chr8.3:48634770-48634789 None:intergenic 35.0%
TCTACCTATTTCAATCGAGA+TGG - chr8.3:48636162-48636181 MS.gene32259:CDS 35.0%
TCTTTGATGGAAAGCAATTC+TGG + chr8.3:48636385-48636404 None:intergenic 35.0%
TGAGATCCTTGAGATGTATA+GGG - chr8.3:48636243-48636262 MS.gene32259:CDS 35.0%
TGTAATCCCTATACATCTCA+AGG + chr8.3:48636252-48636271 None:intergenic 35.0%
TTACAATATCGGGACAAAGA+AGG - chr8.3:48635092-48635111 MS.gene32259:CDS 35.0%
TTAGATGAATTGACTGATGC+GGG + chr8.3:48635426-48635445 None:intergenic 35.0%
TTCATCTAATACTCGTATGG+TGG - chr8.3:48635437-48635456 MS.gene32259:CDS 35.0%
TTGCTCAGATCAGATGAAAT+CGG + chr8.3:48636061-48636080 None:intergenic 35.0%
TTGGCCATGTTTACAATATC+GGG - chr8.3:48635082-48635101 MS.gene32259:CDS 35.0%
TTGTCCCGATATTGTAAACA+TGG + chr8.3:48635089-48635108 None:intergenic 35.0%
TTTGCCATCTCGATTGAAAT+AGG + chr8.3:48636169-48636188 None:intergenic 35.0%
! AAATTCTGCCACTTGAAACT+GGG + chr8.3:48636280-48636299 None:intergenic 35.0%
! AACTTCAAAGGATGTTTCTG+CGG + chr8.3:48635489-48635508 None:intergenic 35.0%
! AGCACAACAATTTTGTACTC+GGG + chr8.3:48634445-48634464 None:intergenic 35.0%
! CAACTTTAAGTTTGTGAAGG+GGG - chr8.3:48634522-48634541 MS.gene32259:CDS 35.0%
! CCAACTTTAAGTTTGTGAAG+GGG - chr8.3:48634521-48634540 MS.gene32259:CDS 35.0%
! CCCAACTTTAAGTTTGTGAA+GGG - chr8.3:48634520-48634539 MS.gene32259:CDS 35.0%
! GATTCCAAAGTTCATCCTTT+TGG - chr8.3:48634948-48634967 MS.gene32259:CDS 35.0%
! TCCCAACTTTAAGTTTGTGA+AGG - chr8.3:48634519-48634538 MS.gene32259:CDS 35.0%
!! AACTTTGGAATCAACTTCTC+GGG + chr8.3:48634940-48634959 None:intergenic 35.0%
!! GAACTTTGGAATCAACTTCT+CGG + chr8.3:48634941-48634960 None:intergenic 35.0%
AAAGACTCTTCCCATTCATG+GGG - chr8.3:48634981-48635000 MS.gene32259:CDS 40.0%
AACTTCTCGGGAAACTGATT+TGG + chr8.3:48634928-48634947 None:intergenic 40.0%
AAGTTGGGAGATGATTTCGA+AGG + chr8.3:48634508-48634527 None:intergenic 40.0%
AAGTTTGTGAAGGGGGATAT+CGG - chr8.3:48634529-48634548 MS.gene32259:CDS 40.0%
ACATCTCAAGGATCTCATTG+TGG + chr8.3:48636240-48636259 None:intergenic 40.0%
ACCAAAAATCCTGATTGGTG+GGG - chr8.3:48635309-48635328 MS.gene32259:CDS 40.0%
ACCGATGAAGATGCTATTGT+TGG - chr8.3:48634766-48634785 MS.gene32259:CDS 40.0%
ACTCATGTCGACAACTCATT+TGG - chr8.3:48634622-48634641 MS.gene32259:CDS 40.0%
AGAGAAACTTGAGTGGAATC+TGG - chr8.3:48636188-48636207 MS.gene32259:CDS 40.0%
AGTTTCGCACCAATCAACAT+TGG + chr8.3:48635688-48635707 None:intergenic 40.0%
ATATCGGGACAAAGAAGGAA+AGG - chr8.3:48635097-48635116 MS.gene32259:CDS 40.0%
ATCTTTGTGATGAAGTTGCG+TGG + chr8.3:48636085-48636104 None:intergenic 40.0%
ATGACAATGTATGCACCCTT+AGG - chr8.3:48636026-48636045 MS.gene32259:CDS 40.0%
ATGGCAAAGAGAAACTTGAG+TGG - chr8.3:48636181-48636200 MS.gene32259:CDS 40.0%
ATGGCATATGGTAGGTCATA+CGG - chr8.3:48634862-48634881 MS.gene32259:intron 40.0%
ATTAGAACCATCCCCATGAA+TGG + chr8.3:48634995-48635014 None:intergenic 40.0%
CACCTGCAGCATTAAAAACA+TGG + chr8.3:48635653-48635672 None:intergenic 40.0%
CATGAATGAACCTTCTGATC+TGG + chr8.3:48634720-48634739 None:intergenic 40.0%
CCAAACAGCAAGAACTTCAA+AGG + chr8.3:48635501-48635520 None:intergenic 40.0%
CCCCTTCACAAACTTAAAGT+TGG + chr8.3:48634524-48634543 None:intergenic 40.0%
CCCGTAATGAGGATATTCTT+CGG + chr8.3:48634370-48634389 None:intergenic 40.0%
CCGAAGAATATCCTCATTAC+GGG - chr8.3:48634367-48634386 MS.gene32259:CDS 40.0%
CGAAGAATATCCTCATTACG+GGG - chr8.3:48634368-48634387 MS.gene32259:CDS 40.0%
CGACCCTTTGCATATTCATA+TGG + chr8.3:48635579-48635598 None:intergenic 40.0%
GAAAAGCCTGCATATATCCT+TGG + chr8.3:48635139-48635158 None:intergenic 40.0%
GAAAGACTCTTCCCATTCAT+GGG - chr8.3:48634980-48634999 MS.gene32259:CDS 40.0%
GAAATGCTTGTCATGGCATA+TGG - chr8.3:48634850-48634869 MS.gene32259:intron 40.0%
GAAGAATATCCTCATTACGG+GGG - chr8.3:48634369-48634388 MS.gene32259:CDS 40.0%
GAAGACAAGCCCAATTTCAT+GGG - chr8.3:48635864-48635883 MS.gene32259:CDS 40.0%
GATCAGAAGCTGAAAAACCT+CGG - chr8.3:48635231-48635250 MS.gene32259:CDS 40.0%
GCAGCATTAAAAACATGGGT+TGG + chr8.3:48635648-48635667 None:intergenic 40.0%
GGGAAATTGTGTGAAAAGCA+AGG - chr8.3:48635549-48635568 MS.gene32259:CDS 40.0%
GTATTGTGAAGATGTTGCTG+AGG - chr8.3:48635029-48635048 MS.gene32259:CDS 40.0%
GTTTCGCACCAATCAACATT+GGG + chr8.3:48635687-48635706 None:intergenic 40.0%
TATCGGGACAAAGAAGGAAA+GGG - chr8.3:48635098-48635117 MS.gene32259:CDS 40.0%
TCCAAACAGCATGACAGTAT+TGG - chr8.3:48636132-48636151 MS.gene32259:CDS 40.0%
TCCAATACTGTCATGCTGTT+TGG + chr8.3:48636136-48636155 None:intergenic 40.0%
TCTTTCCTTGCATAGCCAAA+AGG + chr8.3:48634966-48634985 None:intergenic 40.0%
TCTTTGTGATGAAGTTGCGT+GGG + chr8.3:48636084-48636103 None:intergenic 40.0%
TGACAATGTATGCACCCTTA+GGG - chr8.3:48636027-48636046 MS.gene32259:CDS 40.0%
TGCTATTGTTGGAAACCATG+AGG - chr8.3:48634777-48634796 MS.gene32259:intron 40.0%
TGTTGCTGGCACTTTAACAT+TGG - chr8.3:48635734-48635753 MS.gene32259:CDS 40.0%
TTACCTGTTATCACAACACG+TGG - chr8.3:48634886-48634905 MS.gene32259:CDS 40.0%
TTAGAACCATCCCCATGAAT+GGG + chr8.3:48634994-48635013 None:intergenic 40.0%
TTTCCACGTGTTGTGATAAC+AGG + chr8.3:48634892-48634911 None:intergenic 40.0%
! AATTCTGCCACTTGAAACTG+GGG + chr8.3:48636279-48636298 None:intergenic 40.0%
! CAACAATTTTGTACTCGGGA+TGG + chr8.3:48634441-48634460 None:intergenic 40.0%
! GAAATTCTGCCACTTGAAAC+TGG + chr8.3:48636281-48636300 None:intergenic 40.0%
! GGAATGGAGAGACATTTTGA+AGG - chr8.3:48635384-48635403 MS.gene32259:CDS 40.0%
! TATATGCAGGCTTTTCTCGA+TGG - chr8.3:48635143-48635162 MS.gene32259:CDS 40.0%
! TGTCTTATTAGAAGCCTGCA+AGG - chr8.3:48634687-48634706 MS.gene32259:CDS 40.0%
!! ACCCATGTTTTTAATGCTGC+AGG - chr8.3:48635648-48635667 MS.gene32259:CDS 40.0%
!! AGACATTTTGAAGGATCCGA+AGG - chr8.3:48635393-48635412 MS.gene32259:CDS 40.0%
!! CCTTTGAAGTTCTTGCTGTT+TGG - chr8.3:48635498-48635517 MS.gene32259:CDS 40.0%
!! GATCATCGAGAAAGTATCTC+TGG + chr8.3:48635215-48635234 None:intergenic 40.0%
!! TGAAGTTCTTGCTGTTTGGT+AGG - chr8.3:48635502-48635521 MS.gene32259:CDS 40.0%
!! TTCATCCTTTTGGCTATGCA+AGG - chr8.3:48634958-48634977 MS.gene32259:CDS 40.0%
AAATCGGCATACGAACCCTA+AGG + chr8.3:48636045-48636064 None:intergenic 45.0%
AAATGTCTCTCCATTCCACC+AGG + chr8.3:48635381-48635400 None:intergenic 45.0%
AATCGGCATACGAACCCTAA+GGG + chr8.3:48636044-48636063 None:intergenic 45.0%
ACATTGACCCCAGTTTCAAG+TGG - chr8.3:48636269-48636288 MS.gene32259:CDS 45.0%
ACGTAGCCAAGGATATATGC+AGG - chr8.3:48635130-48635149 MS.gene32259:CDS 45.0%
AGAAGCTGAAAAACCTCGGT+TGG - chr8.3:48635235-48635254 MS.gene32259:CDS 45.0%
AGTTGTTCTTCACAAGGGAG+AGG - chr8.3:48635059-48635078 MS.gene32259:CDS 45.0%
ATCATCAACATCCTCGGATG+AGG + chr8.3:48635357-48635376 None:intergenic 45.0%
ATCGGGACAAAGAAGGAAAG+GGG - chr8.3:48635099-48635118 MS.gene32259:CDS 45.0%
CAAAAATCCTGATTGGTGGG+GGG - chr8.3:48635311-48635330 MS.gene32259:CDS 45.0%
CCAAAAATCCTGATTGGTGG+GGG - chr8.3:48635310-48635329 MS.gene32259:CDS 45.0%
CCAATCAACATTGGGTCTTC+CGG + chr8.3:48635679-48635698 None:intergenic 45.0%
CGAAGCAACAATGAGATGGA+TGG - chr8.3:48636337-48636356 MS.gene32259:CDS 45.0%
GAAGAAGTTGAGAAGCCTCA+TGG + chr8.3:48634795-48634814 None:intergenic 45.0%
GAAGCTGAAAAACCTCGGTT+GGG - chr8.3:48635236-48635255 MS.gene32259:CDS 45.0%
GGAAAGACTCTTCCCATTCA+TGG - chr8.3:48634979-48634998 MS.gene32259:CDS 45.0%
GGAAGACAAGCCCAATTTCA+TGG - chr8.3:48635863-48635882 MS.gene32259:CDS 45.0%
TGCTCGAAGCAACAATGAGA+TGG - chr8.3:48636333-48636352 MS.gene32259:CDS 45.0%
TGCTTGTCATGGCATATGGT+AGG - chr8.3:48634854-48634873 MS.gene32259:intron 45.0%
TGGCAGTGTTCTTGGTTGAT+GGG + chr8.3:48635464-48635483 None:intergenic 45.0%
TGGCATATGGTAGGTCATAC+GGG - chr8.3:48634863-48634882 MS.gene32259:intron 45.0%
TGGGAGATGATTTCGAAGGA+AGG + chr8.3:48634504-48634523 None:intergenic 45.0%
TTAGAAGCCTGCAAGGTTAC+TGG - chr8.3:48634694-48634713 MS.gene32259:CDS 45.0%
TTCCTCCTTGAAACCAATGC+CGG + chr8.3:48635847-48635866 None:intergenic 45.0%
TTGCATCTCATGTTGCCAAC+CGG - chr8.3:48634404-48634423 MS.gene32259:CDS 45.0%
! ATCTGGAACTTCACTAACCC+TGG - chr8.3:48636205-48636224 MS.gene32259:CDS 45.0%
! TTTAATGCTGCAGGTGTCAC+CGG - chr8.3:48635657-48635676 MS.gene32259:CDS 45.0%
! TTTTGTACTCGGGATGGCTT+CGG + chr8.3:48634435-48634454 None:intergenic 45.0%
!! ATGGGGATGGTTCTAATGTG+AGG - chr8.3:48634998-48635017 MS.gene32259:CDS 45.0%
!! CATTCATGTCAGTACCGATG+AGG - chr8.3:48634732-48634751 MS.gene32259:CDS 45.0%
!! CCTGCTTTTGTTGCAGAGTA+CGG + chr8.3:48634826-48634845 None:intergenic 45.0%
!! CTGCTTTTGTTGCAGAGTAC+GGG + chr8.3:48634825-48634844 None:intergenic 45.0%
!! TCAGTACCGATGAGGTCTAT+GGG - chr8.3:48634740-48634759 MS.gene32259:CDS 45.0%
!! TCTTGCTGTTTGGTAGGACA+GGG - chr8.3:48635508-48635527 MS.gene32259:CDS 45.0%
!! TTCTTGCTGTTTGGTAGGAC+AGG - chr8.3:48635507-48635526 MS.gene32259:CDS 45.0%
AAGTGCCAGCAACATTAGCG+CGG + chr8.3:48635728-48635747 None:intergenic 50.0%
ACTCTTCCCATTCATGGGGA+TGG - chr8.3:48634985-48635004 MS.gene32259:CDS 50.0%
AGGAGCGGAAATGCTTGTCA+TGG - chr8.3:48634843-48634862 MS.gene32259:intron 50.0%
AGGTTACTGGCCAGATCAGA+AGG - chr8.3:48634707-48634726 MS.gene32259:CDS 50.0%
CCAGGCATCATCAACATCCT+CGG + chr8.3:48635363-48635382 None:intergenic 50.0%
CCGGAAGACCCAATGTTGAT+TGG - chr8.3:48635676-48635695 MS.gene32259:CDS 50.0%
CCGTACTCTGCAACAAAAGC+AGG - chr8.3:48634823-48634842 MS.gene32259:intron 50.0%
CTCTGCAACAAAAGCAGGAG+CGG - chr8.3:48634828-48634847 MS.gene32259:intron 50.0%
CTTCAGTCCTTCATCCCATG+TGG + chr8.3:48635271-48635290 None:intergenic 50.0%
GGGAGTTATTGACGTAGCCA+AGG - chr8.3:48635119-48635138 MS.gene32259:CDS 50.0%
GTGGCAGTGTTCTTGGTTGA+TGG + chr8.3:48635465-48635484 None:intergenic 50.0%
GTTCTTCACAAGGGAGAGGT+TGG - chr8.3:48635063-48635082 MS.gene32259:CDS 50.0%
GTTGCAGAGTACGGGTTTGT+GGG + chr8.3:48634817-48634836 None:intergenic 50.0%
TACGATGCAGCACATCCAGA+AGG - chr8.3:48635819-48635838 MS.gene32259:CDS 50.0%
TCGAGCAGCGACAATTACCT+TGG + chr8.3:48636320-48636339 None:intergenic 50.0%
TGTTGCAGAGTACGGGTTTG+TGG + chr8.3:48634818-48634837 None:intergenic 50.0%
TTTGGTAGGACAGGGTGGAT+TGG - chr8.3:48635516-48635535 MS.gene32259:CDS 50.0%
! CAAAGGATGTTTCTGCGGAG+TGG + chr8.3:48635484-48635503 None:intergenic 50.0%
! CCCCCACCAATCAGGATTTT+TGG + chr8.3:48635313-48635332 None:intergenic 50.0%
! TGATGCGGGATTTTCGCCTT+CGG + chr8.3:48635412-48635431 None:intergenic 50.0%
!! AGGATGTTGATGATGCCTGG+TGG - chr8.3:48635363-48635382 MS.gene32259:CDS 50.0%
!! CAGTACCGATGAGGTCTATG+GGG - chr8.3:48634741-48634760 MS.gene32259:CDS 50.0%
!! GTCAGTACCGATGAGGTCTA+TGG - chr8.3:48634739-48634758 MS.gene32259:CDS 50.0%
!! GTTGATGATGCCTGGTGGAA+TGG - chr8.3:48635368-48635387 MS.gene32259:CDS 50.0%
AAAACCTCGGTTGGGCTGAG+AGG - chr8.3:48635244-48635263 MS.gene32259:CDS 55.0%
AAACCAATGCCGGAGCCTTC+TGG + chr8.3:48635837-48635856 None:intergenic 55.0%
ACCATCCGCGCTAATGTTGC+TGG - chr8.3:48635720-48635739 MS.gene32259:CDS 55.0%
CACGCTTGAAGAGCAAGCCA+AGG - chr8.3:48636300-48636319 MS.gene32259:CDS 55.0%
CATTGTGGCTCACAGCACCA+GGG + chr8.3:48636225-48636244 None:intergenic 55.0%
GAGAGGACCACATGGGATGA+AGG - chr8.3:48635261-48635280 MS.gene32259:CDS 55.0%
GATCTGGCCAGTAACCTTGC+AGG + chr8.3:48634704-48634723 None:intergenic 55.0%
GCTGATGTGTGCAGAGAGCA+CGG - chr8.3:48635756-48635775 MS.gene32259:CDS 55.0%
GGTCTCCCCATAGACCTCAT+CGG + chr8.3:48634749-48634768 None:intergenic 55.0%
GTGCATTGCTTCCTCATCCG+AGG - chr8.3:48635343-48635362 MS.gene32259:CDS 55.0%
TCATTGTGGCTCACAGCACC+AGG + chr8.3:48636226-48636245 None:intergenic 55.0%
TTGGGCTGAGAGGACCACAT+GGG - chr8.3:48635254-48635273 MS.gene32259:CDS 55.0%
! CTGATGTGTGCAGAGAGCAC+GGG - chr8.3:48635757-48635776 MS.gene32259:CDS 55.0%
! TGCTGTTTGGTAGGACAGGG+TGG - chr8.3:48635511-48635530 MS.gene32259:CDS 55.0%
!! AGGCTCCGGCATTGGTTTCA+AGG - chr8.3:48635839-48635858 MS.gene32259:CDS 55.0%
!! AGGGTGGATTGGAGGTTTGC+TGG - chr8.3:48635527-48635546 MS.gene32259:CDS 55.0%
!! CCGAGGATGTTGATGATGCC+TGG - chr8.3:48635360-48635379 MS.gene32259:CDS 55.0%
!! CTCCGGCATTGGTTTCAAGG+AGG - chr8.3:48635842-48635861 MS.gene32259:CDS 55.0%
!! GGGTGGATTGGAGGTTTGCT+GGG - chr8.3:48635528-48635547 MS.gene32259:CDS 55.0%
!! GGTGGATTGGAGGTTTGCTG+GGG - chr8.3:48635529-48635548 MS.gene32259:CDS 55.0%
ATCCAGCTGCCCCCGTAATG+AGG + chr8.3:48634381-48634400 None:intergenic 60.0%
ATCCTCATTACGGGGGCAGC+TGG - chr8.3:48634376-48634395 MS.gene32259:CDS 60.0%
ATGGCTTCGGACAAGCCGGT+TGG + chr8.3:48634422-48634441 None:intergenic 60.0%
CATCCAGAAGGCTCCGGCAT+TGG - chr8.3:48635831-48635850 MS.gene32259:CDS 60.0%
CTGACATCCCCCCACCAATC+AGG + chr8.3:48635321-48635340 None:intergenic 60.0%
CTGCGGAGTGGCAGTGTTCT+TGG + chr8.3:48635472-48635491 None:intergenic 60.0%
GATTGGTGGGGGGATGTCAG+TGG - chr8.3:48635321-48635340 MS.gene32259:CDS 60.0%
GCAGCACATCCAGAAGGCTC+CGG - chr8.3:48635825-48635844 MS.gene32259:CDS 60.0%
GCCAGCAACATTAGCGCGGA+TGG + chr8.3:48635724-48635743 None:intergenic 60.0%
GTTGGGCTGAGAGGACCACA+TGG - chr8.3:48635253-48635272 MS.gene32259:CDS 60.0%
!! GGTAGGACAGGGTGGATTGG+AGG - chr8.3:48635519-48635538 MS.gene32259:CDS 60.0%
CGGGATGGCTTCGGACAAGC+CGG + chr8.3:48634426-48634445 None:intergenic 65.0%
TGGTCCTCTCAGCCCAACCG+AGG + chr8.3:48635251-48635270 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 48634352 48636447 48634352 ID=MS.gene32259
chr8.3 mRNA 48634352 48636447 48634352 ID=MS.gene32259.t1;Parent=MS.gene32259
chr8.3 exon 48634885 48636447 48634885 ID=MS.gene32259.t1.exon1;Parent=MS.gene32259.t1
chr8.3 CDS 48634885 48636447 48634885 ID=cds.MS.gene32259.t1;Parent=MS.gene32259.t1
chr8.3 exon 48634352 48634795 48634352 ID=MS.gene32259.t1.exon2;Parent=MS.gene32259.t1
chr8.3 CDS 48634352 48634795 48634352 ID=cds.MS.gene32259.t1;Parent=MS.gene32259.t1
Gene Sequence

>MS.gene32259

ATGGCTTCGTATACACCGAAGAATATCCTCATTACGGGGGCAGCTGGATTTATTGCATCTCATGTTGCCAACCGGCTTGTCCGAAGCCATCCCGAGTACAAAATTGTTGTGCTTGATAAACTAGATTATTGTTCTAATTTAAAGAATCTCCTTCCTTCGAAATCATCTCCCAACTTTAAGTTTGTGAAGGGGGATATCGGTAGTGCTGATCTTGTCAACTACTTGCTTATTACGGAGTCGATTGACACGATAATGCACTTTGCTGCTCAAACTCATGTCGACAACTCATTTGGTAATAGCTTTGAGTTCACCAAGAACAACATATATGGAACTCATGTCTTATTAGAAGCCTGCAAGGTTACTGGCCAGATCAGAAGGTTCATTCATGTCAGTACCGATGAGGTCTATGGGGAGACCGATGAAGATGCTATTGTTGGAAACCATGAGGCTTCTCAACTTCTTCCCACAAACCCGTACTCTGCAACAAAAGCAGGAGCGGAAATGCTTGTCATGGCATATGGTAGGTCATACGGGTTACCTGTTATCACAACACGTGGAAACAATGTTTATGGGCCAAATCAGTTTCCCGAGAAGTTGATTCCAAAGTTCATCCTTTTGGCTATGCAAGGAAAGACTCTTCCCATTCATGGGGATGGTTCTAATGTGAGGAGTTATTTGTATTGTGAAGATGTTGCTGAGGCATTTGAAGTTGTTCTTCACAAGGGAGAGGTTGGCCATGTTTACAATATCGGGACAAAGAAGGAAAGGGGAGTTATTGACGTAGCCAAGGATATATGCAGGCTTTTCTCGATGGATCCAGAAGCTAACATTAAATTTGTTGAGAACAGACCGTTTAATGACCAGAGATACTTTCTCGATGATCAGAAGCTGAAAAACCTCGGTTGGGCTGAGAGGACCACATGGGATGAAGGACTGAAGAAAACCATAGAATGGTATACCAAAAATCCTGATTGGTGGGGGGATGTCAGTGGTGCATTGCTTCCTCATCCGAGGATGTTGATGATGCCTGGTGGAATGGAGAGACATTTTGAAGGATCCGAAGGCGAAAATCCCGCATCAGTCAATTCATCTAATACTCGTATGGTGGTCCCATCAACCAAGAACACTGCCACTCCGCAGAAACATCCTTTGAAGTTCTTGCTGTTTGGTAGGACAGGGTGGATTGGAGGTTTGCTGGGGAAATTGTGTGAAAAGCAAGGAATTCCATATGAATATGCAAAGGGTCGTCTAGAAGATCGTGCATCACTCATTTCTGATATTCAGAATGTGAAGCCAACCCATGTTTTTAATGCTGCAGGTGTCACCGGAAGACCCAATGTTGATTGGTGCGAAACTCATAAAACAGAAACCATCCGCGCTAATGTTGCTGGCACTTTAACATTGGCTGATGTGTGCAGAGAGCACGGGATCTTGATGATAAATTATGCTACTGGCTGCATATTTGAGTACGATGCAGCACATCCAGAAGGCTCCGGCATTGGTTTCAAGGAGGAAGACAAGCCCAATTTCATGGGATCTTTCTATTCCAAAACTAAAGCTATGGTAAGTTTCCTTAAATAGAACGATCTTATTTATCACCTTTGCATTATGAATTTTTTTCAAACAGTAACTCTTAATTGAATTTTGATCAGGTTGAGGAACTCTTGAGAGAATATGACAATGTATGCACCCTTAGGGTTCGTATGCCGATTTCATCTGATCTGAGCAACCCACGCAACTTCATCACAAAGATTTCTCGTTATAACAAAGTTGTCAACATTCCAAACAGCATGACAGTATTGGATGAACTTCTACCTATTTCAATCGAGATGGCAAAGAGAAACTTGAGTGGAATCTGGAACTTCACTAACCCTGGTGCTGTGAGCCACAATGAGATCCTTGAGATGTATAGGGATTACATTGACCCCAGTTTCAAGTGGCAGAATTTCACGCTTGAAGAGCAAGCCAAGGTAATTGTCGCTGCTCGAAGCAACAATGAGATGGATGGTGCCAAATTAAAGAAAGAATTCCCAGAATTGCTTTCCATCAAAGAATCACTTATCAAGTATGTCTTCGTGCCAAACAAGAAGAAATAA
Protein sequence

>MS.gene32259.t1

MASYTPKNILITGAAGFIASHVANRLVRSHPEYKIVVLDKLDYCSNLKNLLPSKSSPNFKFVKGDIGSADLVNYLLITESIDTIMHFAAQTHVDNSFGNSFEFTKNNIYGTHVLLEACKVTGQIRRFIHVSTDEVYGETDEDAIVGNHEASQLLPTNPYSATKAGAEMLVMAYGRSYGLPVITTRGNNVYGPNQFPEKLIPKFILLAMQGKTLPIHGDGSNVRSYLYCEDVAEAFEVVLHKGEVGHVYNIGTKKERGVIDVAKDICRLFSMDPEANIKFVENRPFNDQRYFLDDQKLKNLGWAERTTWDEGLKKTIEWYTKNPDWWGDVSGALLPHPRMLMMPGGMERHFEGSEGENPASVNSSNTRMVVPSTKNTATPQKHPLKFLLFGRTGWIGGLLGKLCEKQGIPYEYAKGRLEDRASLISDIQNVKPTHVFNAAGVTGRPNVDWCETHKTETIRANVAGTLTLADVCREHGILMINYATGCIFEYDAAHPEGSGIGFKEEDKPNFMGSFYSKTKAMVEELLREYDNVCTLRVRMPISSDLSNPRNFITKISRYNKVVNIPNSMTVLDELLPISIEMAKRNLSGIWNFTNPGAVSHNEILEMYRDYIDPSFKWQNFTLEEQAKVIVAARSNNEMDGAKLKKEFPELLSIKESLIKYVFVPNKKK