AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073888

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073888.t1 MTR_1g044720 98.975 488 5 0 1 488 32 519 0.0 1005
MS.gene073888.t1 MTR_3g045130 28.571 224 148 5 206 425 287 502 8.85e-23 101
MS.gene073888.t1 MTR_1g072140 24.756 307 221 6 178 480 14 314 6.96e-22 96.7
MS.gene073888.t1 MTR_5g022690 27.703 296 176 12 206 478 195 475 1.72e-19 91.7
MS.gene073888.t1 MTR_4g119620 28.230 209 142 4 191 394 78 283 2.37e-18 86.3
MS.gene073888.t1 MTR_4g119620 23.643 258 143 5 228 478 22 232 1.60e-14 75.1
MS.gene073888.t1 MTR_4g119620 27.041 196 133 6 204 392 133 325 1.53e-12 68.9
MS.gene073888.t1 MTR_2g012630 28.824 170 117 2 226 394 51 217 4.99e-18 88.2
MS.gene073888.t1 MTR_2g012630 26.163 172 124 1 217 388 84 252 6.54e-15 78.2
MS.gene073888.t1 MTR_2g012630 27.972 143 94 2 204 343 113 249 1.67e-12 70.5
MS.gene073888.t1 MTR_2g012630 28.824 170 117 2 226 394 51 217 5.01e-18 87.8
MS.gene073888.t1 MTR_2g012630 26.163 172 124 1 217 388 84 252 6.53e-15 78.2
MS.gene073888.t1 MTR_2g012630 27.972 143 94 2 204 343 113 249 1.57e-12 70.5
MS.gene073888.t1 MTR_6g004040 26.818 220 155 3 202 420 28 242 5.36e-18 87.8
MS.gene073888.t1 MTR_6g004040 24.257 202 150 1 193 394 61 259 2.01e-15 79.7
MS.gene073888.t1 MTR_6g004040 23.671 207 144 6 277 478 15 212 1.42e-11 67.4
MS.gene073888.t1 MTR_3g106080 28.191 188 131 2 204 390 432 616 1.49e-16 82.8
MS.gene073888.t1 MTR_3g106080 27.632 228 156 5 206 429 389 611 3.68e-16 81.6
MS.gene073888.t1 MTR_3g106080 28.834 163 113 1 206 368 476 635 1.67e-15 79.7
MS.gene073888.t1 MTR_1g011610 26.738 187 131 3 205 390 627 808 3.10e-14 75.9
MS.gene073888.t1 MTR_1g011610 26.738 187 131 3 205 390 636 817 3.15e-14 75.9
MS.gene073888.t1 MTR_3g107910 27.807 187 129 3 205 390 642 823 3.65e-14 75.9
MS.gene073888.t1 MTR_3g107910 27.807 187 129 3 205 390 635 816 4.19e-14 75.5
MS.gene073888.t1 MTR_8g106790 25.188 266 173 9 136 390 12 262 9.87e-14 72.4
MS.gene073888.t1 MTR_4g095058 21.576 533 326 18 2 478 35 531 3.65e-13 72.0
MS.gene073888.t1 MTR_4g095058 27.612 268 174 9 187 444 284 541 1.63e-12 70.1
MS.gene073888.t1 MTR_4g095058 22.507 351 229 9 162 478 192 533 4.40e-13 72.0
MS.gene073888.t1 MTR_4g095058 27.612 268 174 9 187 444 286 543 1.43e-12 70.5
MS.gene073888.t1 MTR_2g436510 28.049 164 114 2 229 391 166 326 1.07e-12 70.5
MS.gene073888.t1 MTR_1g050340 24.727 275 194 8 205 478 523 785 1.10e-12 70.9
MS.gene073888.t1 MTR_1g050340 24.727 275 194 8 205 478 524 786 1.11e-12 70.9
MS.gene073888.t1 MTR_7g086360 25.573 262 178 9 226 478 48 301 1.90e-12 68.9
MS.gene073888.t1 MTR_7g058460 25.668 187 133 4 205 390 521 702 2.65e-12 69.7
MS.gene073888.t1 MTR_2g014020 25.871 201 142 4 189 388 246 440 5.89e-12 68.2
MS.gene073888.t1 MTR_8g038550 23.858 197 142 4 206 399 198 389 6.24e-12 68.6
MS.gene073888.t1 MTR_3g116500 27.586 203 128 8 206 389 122 324 9.68e-12 67.0
MS.gene073888.t1 MTR_4g113080 23.913 184 134 3 205 388 518 695 1.01e-11 67.8
MS.gene073888.t1 MTR_3g116500 27.941 204 126 9 206 389 12 214 1.10e-11 65.9
MS.gene073888.t1 MTR_4g105700 26.455 189 126 5 217 395 44 229 1.60e-11 67.4
MS.gene073888.t1 MTR_4g105700 28.834 163 112 2 227 388 494 653 7.53e-11 65.1
MS.gene073888.t1 MTR_3g075400 26.178 191 135 4 206 394 240 426 2.05e-11 66.6
MS.gene073888.t1 MTR_3g052220 25.098 255 169 9 240 478 221 469 2.94e-11 65.9
MS.gene073888.t1 MTR_5g016350 22.692 260 152 5 188 399 291 549 3.15e-11 66.2
MS.gene073888.t1 MTR_5g016350 22.692 260 152 5 188 399 227 485 3.36e-11 65.9
MS.gene073888.t1 MTR_7g076230 25.000 252 168 8 235 478 286 524 7.97e-11 65.1
MS.gene073888.t1 MTR_7g104550 23.077 208 138 5 202 387 80 287 8.05e-11 63.9
MS.gene073888.t1 MTR_1g072740 22.394 259 171 9 224 466 284 528 8.38e-11 64.7
MS.gene073888.t1 MTR_5g016370 21.293 263 190 6 224 478 297 550 9.64e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073888.t1 AT2G33340 73.663 486 120 4 1 484 34 513 0.0 755
MS.gene073888.t1 AT2G33340 73.663 486 120 4 1 484 34 513 0.0 755
MS.gene073888.t1 AT1G04510 72.764 492 125 4 1 489 34 519 0.0 744
MS.gene073888.t1 AT1G04510 72.561 492 126 4 1 489 34 519 0.0 743
MS.gene073888.t1 AT2G33340 73.846 455 111 4 1 453 34 482 0.0 704
MS.gene073888.t1 AT2G41500 27.682 289 192 9 205 488 269 545 9.26e-28 117
MS.gene073888.t1 AT2G41500 27.682 289 192 9 205 488 269 545 9.26e-28 117
MS.gene073888.t1 AT3G49660 29.412 204 134 6 198 394 76 276 8.31e-18 84.7
MS.gene073888.t1 AT3G49660 24.436 266 191 5 221 483 59 317 1.06e-16 81.3
MS.gene073888.t1 AT3G49660 25.463 216 150 5 228 437 19 229 2.93e-14 73.9
MS.gene073888.t1 AT5G23430 25.871 201 145 2 202 401 28 225 2.86e-17 85.5
MS.gene073888.t1 AT5G23430 23.267 202 152 1 193 394 61 259 6.29e-14 75.1
MS.gene073888.t1 AT5G23430 25.871 201 145 2 202 401 28 225 2.86e-17 85.5
MS.gene073888.t1 AT5G23430 23.267 202 152 1 193 394 61 259 6.29e-14 75.1
MS.gene073888.t1 AT5G23430 25.871 201 145 2 202 401 28 225 2.91e-17 85.5
MS.gene073888.t1 AT5G23430 23.267 202 152 1 193 394 61 259 6.52e-14 75.1
MS.gene073888.t1 AT5G08390 24.615 260 174 10 226 478 9 253 4.83e-17 84.7
MS.gene073888.t1 AT5G08390 25.773 194 140 2 202 394 28 218 9.03e-17 84.0
MS.gene073888.t1 AT5G08390 22.772 202 153 1 193 394 61 259 2.69e-13 73.2
MS.gene073888.t1 AT5G56130 25.828 302 163 10 231 479 18 311 2.51e-16 80.1
MS.gene073888.t1 AT1G61210 26.020 196 139 3 226 420 51 241 2.56e-15 79.7
MS.gene073888.t1 AT1G61210 24.419 172 127 1 217 388 84 252 9.41e-13 71.2
MS.gene073888.t1 AT1G61210 25.463 216 146 7 268 478 6 211 2.56e-12 70.1
MS.gene073888.t1 AT1G11160 22.780 259 186 8 223 478 5 252 2.62e-15 79.3
MS.gene073888.t1 AT1G11160 25.581 172 125 1 217 388 84 252 1.18e-14 77.4
MS.gene073888.t1 AT5G25150 22.985 335 217 9 134 443 305 623 2.76e-15 79.0
MS.gene073888.t1 AT1G11160 22.780 259 186 8 223 478 5 252 2.86e-15 79.3
MS.gene073888.t1 AT1G11160 25.581 172 125 1 217 388 84 252 1.24e-14 77.4
MS.gene073888.t1 AT1G61210 26.020 196 139 3 226 420 51 241 2.89e-15 79.3
MS.gene073888.t1 AT1G61210 24.419 172 127 1 217 388 84 252 1.16e-12 71.2
MS.gene073888.t1 AT1G61210 25.463 216 146 7 268 478 6 211 3.05e-12 69.7
MS.gene073888.t1 AT5G25150 22.985 335 217 9 134 443 305 623 2.97e-15 79.0
MS.gene073888.t1 AT1G11160 22.780 259 186 8 223 478 5 252 2.99e-15 79.3
MS.gene073888.t1 AT1G11160 25.581 172 125 1 217 388 84 252 1.35e-14 77.4
MS.gene073888.t1 AT1G61210 26.020 196 139 3 226 420 51 241 3.24e-15 79.3
MS.gene073888.t1 AT1G61210 24.419 172 127 1 217 388 84 252 1.28e-12 70.9
MS.gene073888.t1 AT1G61210 25.463 216 146 7 268 478 6 211 3.54e-12 69.7
MS.gene073888.t1 AT4G02730 25.322 233 161 6 207 434 17 241 5.74e-15 76.6
MS.gene073888.t1 AT4G02730 21.311 244 187 4 204 445 56 296 1.84e-12 68.9
MS.gene073888.t1 AT1G11160 25.581 172 125 1 217 388 42 210 1.09e-14 77.4
MS.gene073888.t1 AT1G11160 22.381 210 155 5 270 478 8 210 2.84e-14 76.3
MS.gene073888.t1 AT1G11160 24.742 194 140 3 228 420 11 199 5.57e-14 75.1
MS.gene073888.t1 AT1G18080 25.888 197 141 3 198 390 68 263 6.91e-14 73.2
MS.gene073888.t1 AT2G32700 24.390 205 147 5 188 390 526 724 1.21e-13 73.9
MS.gene073888.t1 AT2G32700 24.390 205 147 5 188 390 505 703 1.30e-13 73.9
MS.gene073888.t1 AT2G32700 24.390 205 147 5 188 390 507 705 1.33e-13 73.9
MS.gene073888.t1 AT2G32700 24.390 205 147 5 188 390 507 705 1.33e-13 73.9
MS.gene073888.t1 AT2G32700 24.390 205 147 5 188 390 507 705 1.33e-13 73.9
MS.gene073888.t1 AT2G32700 24.390 205 147 5 188 390 507 705 1.33e-13 73.9
MS.gene073888.t1 AT2G32700 24.390 205 147 5 188 390 507 705 1.33e-13 73.9
MS.gene073888.t1 AT4G15900 28.049 164 114 2 229 391 172 332 1.41e-13 73.2
MS.gene073888.t1 AT4G15900 27.556 225 150 7 193 413 262 477 3.36e-11 65.9
MS.gene073888.t1 AT5G67320 23.372 261 150 6 187 399 321 579 3.75e-13 72.4
MS.gene073888.t1 AT5G13480 24.771 218 118 6 225 399 103 317 2.08e-12 70.1
MS.gene073888.t1 AT5G13480 26.923 156 109 3 194 347 240 392 1.42e-11 67.4
MS.gene073888.t1 AT5G13480 24.771 218 118 6 225 399 126 340 2.20e-12 70.1
MS.gene073888.t1 AT5G13480 26.923 156 109 3 194 347 263 415 1.55e-11 67.4
MS.gene073888.t1 AT5G13480 24.771 218 118 6 225 399 103 317 2.37e-12 69.7
MS.gene073888.t1 AT5G13480 26.923 156 109 3 194 347 240 392 1.65e-11 67.0
MS.gene073888.t1 AT5G13480 24.771 218 118 6 225 399 115 329 2.48e-12 69.7
MS.gene073888.t1 AT5G13480 26.923 156 109 3 194 347 252 404 1.63e-11 67.0
MS.gene073888.t1 AT3G18130 24.876 201 137 6 198 390 68 262 2.73e-12 68.2
MS.gene073888.t1 AT1G48630 24.876 201 137 6 198 390 68 262 2.75e-12 68.2
MS.gene073888.t1 AT3G16650 25.403 248 168 6 155 391 84 325 5.27e-12 68.2
MS.gene073888.t1 AT1G10580 23.413 252 177 9 224 466 273 517 5.65e-12 68.6
MS.gene073888.t1 AT4G32551 25.668 187 133 5 205 390 703 884 6.21e-12 68.6
MS.gene073888.t1 AT4G32551 25.668 187 133 5 205 390 665 846 7.10e-12 68.6
MS.gene073888.t1 AT5G16750 27.072 181 122 4 217 390 44 221 7.24e-12 68.6
MS.gene073888.t1 AT5G16750 26.994 163 115 2 227 388 493 652 3.15e-11 66.6
MS.gene073888.t1 AT5G16750 22.652 181 130 2 174 347 475 652 4.62e-11 65.9
MS.gene073888.t1 AT2G43770 25.362 276 161 11 226 478 46 299 1.27e-11 66.2
MS.gene073888.t1 AT2G43770 24.648 284 193 12 205 477 67 340 2.44e-11 65.5
MS.gene073888.t1 AT1G62020 23.913 230 139 7 201 397 61 287 5.49e-11 65.9

Find 104 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATCAATCGAATAGGATTT+TGG 0.110325 1.2:-27693611 None:intergenic
GACTGCAATGCTGCTCTTTC+CGG 0.154596 1.2:-27690384 MS.gene073888:CDS
GCATTAGCTGTCACAGAATT+TGG 0.171506 1.2:+27690687 None:intergenic
GACCGGAATCTTCGAATATT+TGG 0.195869 1.2:-27685780 MS.gene073888:CDS
ACAGAATTTGGAGTAGAAAT+AGG 0.203246 1.2:+27690699 None:intergenic
CATCCCGATGGTCTTATTCT+TGG 0.266936 1.2:-27687135 MS.gene073888:CDS
AAAACTCTTCCTGATTTATC+TGG 0.271266 1.2:-27685982 MS.gene073888:CDS
GTGCAGGCAGCAAGCATTCC+TGG 0.291861 1.2:-27693360 MS.gene073888:CDS
TCTTGATATCCTTCATTCTA+AGG 0.302903 1.2:-27690079 MS.gene073888:intron
TTTGGGAAGTGTCCAGTTAC+AGG 0.305772 1.2:-27693593 MS.gene073888:CDS
CGTGATTGCAAGGCTTAAAA+AGG 0.320139 1.2:-27690769 MS.gene073888:CDS
GGATCCTACCTTGCAGTTGC+AGG 0.322521 1.2:-27686565 MS.gene073888:CDS
ATTTCAGATTCCTGCGACTT+TGG 0.324712 1.2:-27690181 MS.gene073888:intron
CAGATTCTCTTGTTAAGATC+TGG 0.328133 1.2:-27687101 MS.gene073888:CDS
ACTGCTAAGTATTCTGAATC+TGG 0.329045 1.2:+27685813 None:intergenic
GTTGGAGTTGCTGAGTCATA+TGG 0.342835 1.2:+27686711 None:intergenic
CAGTTGCAGGCTCTGATGTT+AGG 0.348094 1.2:-27686552 MS.gene073888:intron
GCAAATGTTGCTAAGTTTGA+TGG 0.357923 1.2:-27686969 MS.gene073888:CDS
GGAATGCTTGCTGCCTGCAC+TGG 0.360377 1.2:+27693363 None:intergenic
CTCATGATGGTGTAAAGCTT+TGG 0.362749 1.2:-27686770 MS.gene073888:CDS
TTTGTGACTGCATCACTTGA+TGG 0.370664 1.2:-27687477 MS.gene073888:CDS
TGCAGTCACAAAGTAATTAT+TGG 0.371362 1.2:+27687488 None:intergenic
CTTGCTGCCTGCACTGGTCT+AGG 0.374275 1.2:+27693369 None:intergenic
ACTGCAATGCTGCTCTTTCC+GGG 0.374696 1.2:-27690383 MS.gene073888:CDS
GGATGTAATTTCTTCGCATT+AGG 0.388848 1.2:+27690441 None:intergenic
ACTCAGCGGTCACTCTAAAA+AGG 0.394859 1.2:-27689796 MS.gene073888:intron
AAGCGTGAAGTTTGTAGGTC+AGG 0.396085 1.2:-27689690 MS.gene073888:CDS
CTTAAACAAAACTAGCAAAC+AGG 0.402528 1.2:-27690112 MS.gene073888:CDS
CTTCCTGATTTATCTGGAAC+AGG 0.403899 1.2:-27685976 MS.gene073888:intron
CAGGGTGAATCTATCATAAC+TGG 0.405085 1.2:-27689671 MS.gene073888:CDS
GGCCAAATATTCGAAGATTC+CGG 0.405806 1.2:+27685778 None:intergenic
AAATCCACATGTGTAAAATT+CGG 0.407717 1.2:-27685837 MS.gene073888:CDS
GATATTGTGCCGATCAAAAC+AGG 0.410359 1.2:-27693551 MS.gene073888:CDS
GTAAGCGGCTCTCCTGTAAC+TGG 0.411774 1.2:+27693581 None:intergenic
TCATGATGGTGTAAAGCTTT+GGG 0.421276 1.2:-27686769 MS.gene073888:CDS
CTGCTTGCCGCGTGATTGCA+AGG 0.421314 1.2:-27690779 MS.gene073888:CDS
TATTGCAGACAGTTCGTTTG+TGG 0.428520 1.2:-27689224 MS.gene073888:intron
CTTCGAATATTTGGCCAACC+TGG 0.429124 1.2:-27685771 MS.gene073888:CDS
CATAACTGGTTCAGCAGATA+AGG 0.431703 1.2:-27689657 MS.gene073888:intron
TGTTAGCTCAGAAATTATAC+TGG 0.433157 1.2:+27690407 None:intergenic
TTACAGGACATAATCGCAAC+TGG 0.446375 1.2:-27689876 MS.gene073888:intron
ATTATACTGGAAGATATTCC+AGG 0.453120 1.2:+27690420 None:intergenic
ATACTTAGCAGTTGCATCAA+TGG 0.456401 1.2:-27685802 MS.gene073888:CDS
CAGAATTTGGAGTAGAAATA+GGG 0.462176 1.2:+27690700 None:intergenic
CTGCATCACTTGATGGCAGC+TGG 0.462700 1.2:-27687470 MS.gene073888:CDS
TACAGGAGAGCCGCTTACTC+TGG 0.463440 1.2:-27693576 MS.gene073888:CDS
AGATTCTCTTGTTAAGATCT+GGG 0.470622 1.2:-27687100 MS.gene073888:CDS
GAGTCAATACCAGAGTTTGT+TGG 0.471796 1.2:+27686693 None:intergenic
GCAAGCATTCCTGGCATGCT+TGG 0.474027 1.2:-27693351 MS.gene073888:CDS
GTAACAAGCGTGAAGTTTGT+AGG 0.474487 1.2:-27689695 MS.gene073888:CDS
GTAGTATCCATTCTCAGAAA+AGG 0.476806 1.2:+27686920 None:intergenic
ATGCTCCTGTTCATAGCAAT+GGG 0.482893 1.2:-27690668 MS.gene073888:CDS
AGACAGTTCGTTTGTGGCAA+GGG 0.486431 1.2:-27689218 MS.gene073888:CDS
AGCTGCAGATGTATAGCCTT+CGG 0.487652 1.2:+27687161 None:intergenic
ATGCTGCTCTTTCCGGGAAG+AGG 0.487878 1.2:-27690377 MS.gene073888:CDS
CTCATTTATAGGAATGGGAT+GGG 0.490049 1.2:-27693106 MS.gene073888:intron
GATGGTCTTATTCTTGGAAC+TGG 0.491366 1.2:-27687129 MS.gene073888:CDS
CAGACAGTTCGTTTGTGGCA+AGG 0.499376 1.2:-27689219 MS.gene073888:CDS
AAAGTAATACCTTAGAATGA+AGG 0.500411 1.2:+27690070 None:intergenic
AATGCTCCTGTTCATAGCAA+TGG 0.505400 1.2:-27690669 MS.gene073888:CDS
TGGAACATTCCAAGCATGCC+AGG 0.505625 1.2:+27693342 None:intergenic
AGCTGCTGATGACGAGGAGT+TGG 0.507322 1.2:-27690466 MS.gene073888:CDS
CCTCATTTATAGGAATGGGA+TGG 0.507870 1.2:-27693107 MS.gene073888:intron
TTGGAGTTGCTGAGTCATAT+GGG 0.509156 1.2:+27686712 None:intergenic
GTACCTGTTCCAGATAAATC+AGG 0.512887 1.2:+27685973 None:intergenic
CAAAGTAATTATTGGTGGCA+TGG 0.525985 1.2:+27687496 None:intergenic
CTAAGTTTGATGGACATGTC+GGG 0.530267 1.2:-27686959 MS.gene073888:CDS
AGTGAAGCCTAGACCAGTGC+AGG 0.532479 1.2:-27693376 MS.gene073888:CDS
AGCGTGAAGTTTGTAGGTCA+GGG 0.533196 1.2:-27689689 MS.gene073888:CDS
AAAAGGAACGAGACGAAGCT+AGG 0.533326 1.2:-27690752 MS.gene073888:CDS
TTGTCAGAAATATCTTCGCC+AGG 0.533777 1.2:+27685753 None:intergenic
GTTCCAAGAATAAGACCATC+GGG 0.534047 1.2:+27687132 None:intergenic
TAACTGCAAACAGATCTTGA+AGG 0.534904 1.2:-27689178 MS.gene073888:CDS
GTTCATAGCAATGGGAAAAG+AGG 0.541436 1.2:-27690660 MS.gene073888:intron
TTGCAAATGTGAAATCTGAA+TGG 0.541920 1.2:-27686014 MS.gene073888:CDS
CAGAAATATCTTCGCCAGGT+TGG 0.544143 1.2:+27685757 None:intergenic
GTAGCTAATACTTGTCCTGA+TGG 0.546408 1.2:+27689819 None:intergenic
TTGTGGCAAGGGTCAGATGA+TGG 0.550255 1.2:-27689207 MS.gene073888:CDS
TCAACAGAAGCCAAAGTCGC+AGG 0.550956 1.2:+27690171 None:intergenic
TTCAGGAACTTGTCTGACTC+AGG 0.555913 1.2:-27687430 MS.gene073888:intron
CTGCTAAGTATTCTGAATCT+GGG 0.560624 1.2:+27685814 None:intergenic
AGCTAGGTCTATACTTGCAC+AGG 0.561984 1.2:-27690736 MS.gene073888:CDS
TAGGTTTCTGACTCTTCCGA+AGG 0.569370 1.2:-27687177 MS.gene073888:CDS
CTATACTTGCACAGGCAGAA+AGG 0.575588 1.2:-27690728 MS.gene073888:CDS
TAGCAGTTGCATCAATGGAC+CGG 0.577692 1.2:-27685797 MS.gene073888:CDS
TCAGCAACTCCAACAAACTC+TGG 0.577891 1.2:-27686702 MS.gene073888:intron
AGAGCCTGCAACTGCAAGGT+AGG 0.584256 1.2:+27686561 None:intergenic
GTTAAGTCATGCTCTATACC+AGG 0.586754 1.2:-27693030 MS.gene073888:intron
AATGCGAAGAAATTACATCC+TGG 0.591581 1.2:-27690438 MS.gene073888:CDS
AGTCACAAAGTAATTATTGG+TGG 0.592605 1.2:+27687491 None:intergenic
GCTAAGTTTGATGGACATGT+CGG 0.592762 1.2:-27686960 MS.gene073888:CDS
AGTTCCAAGAATAAGACCAT+CGG 0.599921 1.2:+27687131 None:intergenic
CTAATACTTGTCCTGATGGA+CGG 0.602167 1.2:+27689823 None:intergenic
CATCAGAGCCTGCAACTGCA+AGG 0.620846 1.2:+27686557 None:intergenic
ACAATATCATCCAGAGTAAG+CGG 0.631161 1.2:+27693566 None:intergenic
AGCAGCAGTTGCATACAGCA+AGG 0.634251 1.2:-27693058 MS.gene073888:CDS
TGATAGACCGCAGCTCATGA+TGG 0.652388 1.2:-27686783 MS.gene073888:intron
TGTGCCGATCAAAACAGGCA+AGG 0.675176 1.2:-27693546 MS.gene073888:intron
TTAAACAAAACTAGCAAACA+GGG 0.680512 1.2:-27690111 MS.gene073888:CDS
CTTTACACCATCATGAGCTG+CGG 0.684333 1.2:+27686776 None:intergenic
GCAGTGGAGTTTGACCACAG+TGG 0.700459 1.2:-27686586 MS.gene073888:intron
TGAGAATGGATACTACCTTG+CGG 0.717359 1.2:-27686913 MS.gene073888:intron
CAAGTATTAGCTACACTCAG+CGG 0.734442 1.2:-27689810 MS.gene073888:CDS
CTGCAAGGTAGGATCCACTG+TGG 0.800585 1.2:+27686572 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr1.2 gene 27685746 27693618 27685746 ID=MS.gene073888
chr1.2 mRNA 27685746 27693618 27685746 ID=MS.gene073888.t1;Parent=MS.gene073888
chr1.2 exon 27693547 27693618 27693547 ID=MS.gene073888.t1.exon1;Parent=MS.gene073888.t1
chr1.2 CDS 27693547 27693618 27693547 ID=cds.MS.gene073888.t1;Parent=MS.gene073888.t1
chr1.2 exon 27693338 27693400 27693338 ID=MS.gene073888.t1.exon2;Parent=MS.gene073888.t1
chr1.2 CDS 27693338 27693400 27693338 ID=cds.MS.gene073888.t1;Parent=MS.gene073888.t1
chr1.2 exon 27693031 27693117 27693031 ID=MS.gene073888.t1.exon3;Parent=MS.gene073888.t1
chr1.2 CDS 27693031 27693117 27693031 ID=cds.MS.gene073888.t1;Parent=MS.gene073888.t1
chr1.2 exon 27690661 27690808 27690661 ID=MS.gene073888.t1.exon4;Parent=MS.gene073888.t1
chr1.2 CDS 27690661 27690808 27690661 ID=cds.MS.gene073888.t1;Parent=MS.gene073888.t1
chr1.2 exon 27690365 27690486 27690365 ID=MS.gene073888.t1.exon5;Parent=MS.gene073888.t1
chr1.2 CDS 27690365 27690486 27690365 ID=cds.MS.gene073888.t1;Parent=MS.gene073888.t1
chr1.2 exon 27690080 27690196 27690080 ID=MS.gene073888.t1.exon6;Parent=MS.gene073888.t1
chr1.2 CDS 27690080 27690196 27690080 ID=cds.MS.gene073888.t1;Parent=MS.gene073888.t1
chr1.2 exon 27689797 27689892 27689797 ID=MS.gene073888.t1.exon7;Parent=MS.gene073888.t1
chr1.2 CDS 27689797 27689892 27689797 ID=cds.MS.gene073888.t1;Parent=MS.gene073888.t1
chr1.2 exon 27689658 27689717 27689658 ID=MS.gene073888.t1.exon8;Parent=MS.gene073888.t1
chr1.2 CDS 27689658 27689717 27689658 ID=cds.MS.gene073888.t1;Parent=MS.gene073888.t1
chr1.2 exon 27689164 27689238 27689164 ID=MS.gene073888.t1.exon9;Parent=MS.gene073888.t1
chr1.2 CDS 27689164 27689238 27689164 ID=cds.MS.gene073888.t1;Parent=MS.gene073888.t1
chr1.2 exon 27687431 27687535 27687431 ID=MS.gene073888.t1.exon10;Parent=MS.gene073888.t1
chr1.2 CDS 27687431 27687535 27687431 ID=cds.MS.gene073888.t1;Parent=MS.gene073888.t1
chr1.2 exon 27687086 27687196 27687086 ID=MS.gene073888.t1.exon11;Parent=MS.gene073888.t1
chr1.2 CDS 27687086 27687196 27687086 ID=cds.MS.gene073888.t1;Parent=MS.gene073888.t1
chr1.2 exon 27686914 27686991 27686914 ID=MS.gene073888.t1.exon12;Parent=MS.gene073888.t1
chr1.2 CDS 27686914 27686991 27686914 ID=cds.MS.gene073888.t1;Parent=MS.gene073888.t1
chr1.2 exon 27686703 27686799 27686703 ID=MS.gene073888.t1.exon13;Parent=MS.gene073888.t1
chr1.2 CDS 27686703 27686799 27686703 ID=cds.MS.gene073888.t1;Parent=MS.gene073888.t1
chr1.2 exon 27686553 27686604 27686553 ID=MS.gene073888.t1.exon14;Parent=MS.gene073888.t1
chr1.2 CDS 27686553 27686604 27686553 ID=cds.MS.gene073888.t1;Parent=MS.gene073888.t1
chr1.2 exon 27685977 27686047 27685977 ID=MS.gene073888.t1.exon15;Parent=MS.gene073888.t1
chr1.2 CDS 27685977 27686047 27685977 ID=cds.MS.gene073888.t1;Parent=MS.gene073888.t1
chr1.2 exon 27685746 27685861 27685746 ID=MS.gene073888.t1.exon16;Parent=MS.gene073888.t1
chr1.2 CDS 27685746 27685861 27685746 ID=cds.MS.gene073888.t1;Parent=MS.gene073888.t1
Gene Sequence

>MS.gene073888

GATTTTGGGAAGTGTCCAGTTACAGGAGAGCCGCTTACTCTGGATGATATTGTGCCGATCAAAACAGGCAAGGTATGTTGGTAATGGTATTAGTTTCATGCTTTCTATATTGAGTGTGCAGTTTATAAGTGGACTAAGCCGTTCATGTTAGCCGTGGTTCTTTTATTTTCCTATGTGTATTTGCAGTTTGATAATTATCAGGATAATTTGTCATGCAGATAGTGAAGCCTAGACCAGTGCAGGCAGCAAGCATTCCTGGCATGCTTGGAATGTTCCAAAATGTATGTATGTATAGAATTGAGGCTTACAATATTGATATACGATGACTTTGTTTTAGACTGTTTACTGTAGAAGTTCTTAAAATTTATTTATAAGTTAATTGTTCCAGGAATTTAATGGTGGTTTCTGTTAATAAGTTTTCCTGACAAATACTTGGAAGGAATGCTTAGAAATCTGTAATATCTCTACTCATTACAAATAAATTTTTTCCCTCATTTATAGGAATGGGATGGGTTGATGCTATCTAATTTTGCATTGGAGCAGCAGTTGCATACAGCAAGGCAAGAGTTAAGTCATGCTCTATACCAGGTAAGGGTAACAAAATTATTGATCCAACAAATTATTATCTTCTACAGTTATGTCCCAATTGGTGGAATATGTTTGCAGCCTAAAACCGGGTTATTAATATTTCCTATAATTTATAATTGGTTTCTTGTAAGATTCGATTGGTCTCTTACCGATTTGTTGGTGTAAGTTTTCCATCCTCTTGAATTTCATTATTAAATCAAAATGATTTTTTTCTTTTCTGGATTTCCCATCACAACATGATAATGTACGGGTTACAAAGTTTGCCCTTGAGTTTACAATCCCCAAACCTTCCTGGTCTATATCTCTTAGCTTTTACGGCCTGTTTGGTGCGCAGGATAAGAAGACATGATATGATAACACTGTCATATCCTGCTTAAATCCGTTGCTTGGGTGCATCAGTAGGAAATGATAAAATAAACCTATTACTTATCCTATCCTATTGCTTCTTTGTAATTTTAATCCTTAGTTTTTGGTGGGTTAGCAACCTGATAGCATAAAGCTATCAAACGCGCATCCTCTATTCCTCAGTAAACCTCTTTCGACTCTCTGCTTCTACGTTTTTCATGTTATATTCTTTTTTTCATTTTTTTCTTGAATTTCTTCCCAATCGATATCAATTCCAATCAATTTTCTTCATGTTTTGTTCCTCCAATATATTTTCTTCCCAATCAATTTGGCTGTTGCACCTGAATTGGAAAATACCATTTACGATTTTTCACTATTTTCTTCATATGAAAATTGGCAGTTTTGTGTAATTGATTTTCTGCAATCGATTTGCTTCCCAATGTTACTCCAATCATTTTTGCTTCCCAATGTTATGTTTTGTGCAACCGATTTTCTGGACAATTTTTTTTCCATAATTCTTTATAAATTTCTTCATAATTTTGTTCCCAATCTTTCTGAATTACGATTTTCTTCATAATTTTTTTCATAAAATTACAATCAATATCAATTCCATCATTTTCTTCCCAATCTTGTTCCCAATCGATTTAATTATGTGTGTTGTTATTTTTATCATGTCAATAAAACCTTTGCGCCAAACACATGATAGAATAAAATAATGCTATCATATTTGTTATCATGTGTGCACCAAACACATGATAGGATAAATGTCTATCATGTCACTATCATATCCTATCTTTATCCATTTTCTAATCCTATCATGTGTCTATCATATCATACGCACCAAACGGACCCTTAAGGTTCTCATTTATGCGATAAATTTTTCCATTTGAGATTCATTTTATATATTCTCAGATAAGGATAGTGAACATGTCTTTTGTTTGTTAAAACTCCTATTTTATATATTAAGGCGTAAACTTTCAGCATCACATAGAACTTCTTCTCAAAATCAATTTTTAATTGTTGCGATTTCTTTTACTATTGGAAAATAAGGATAGTGAACATGTCTTTTGTTTGTTAAAACTCCTATTTTTATATATTAAGGTTTAAACTTTCAGCATCACATAGAACTTCTCAAAATCAATTTTTAATTGTTGCGATTTCTTTTACTATTGGAAAATAAGAAATTTGTGATTTTTAACTTAGTTACCAAATTCAGTCACTCTCCCTACAAATCGCATACTGGGATTTGTGTTCATGTTCACGGTATCTTCATGTTACAATTCGTCAGGGAAGATGTGTTGGGAGTTTGGGGTGCACCTTGGCCTTCTAAGAGTAATCGAAGGGAGAAGGGAAAATGAGAAGGGAGTCTGTTCTCACTGAAAAAGGTTTAAGATAATAACTAATCAAAGGGGAATGTAAAAGATAGTCTTGTTTTTGATTAAAAAAAAAAGACAGGCTTGTTTACACATTACAACTAAGTACAGGTGAAGTTGGAGAGTATTTAAATGGCGAGAAGTTTTTTTGGACGGGAAAGATTGATGACAAAGGAAATGTGAAAGATGGTGTGGGAAGTTTCAACAGTAAACATAAAAGAGAAATTATGCCAGTAAGAGAGGGGGATGGTTCAGGTGGTAGTGCTGTAGTAGGAAGAGAAATCAATTTAAAATTTTAAATGATAAATTTAATATTTGGTTTAGCCTTTTTTCATTTGTTTGACTTATGCATTAATATTTATTATTTTCTCCAACTGTTCTTTCCTGGTATGATTTCTTATCTTGATAAGATCCTGTGCCTGATAATGATTTCAAATTTTCACAACTTGTTCCTCTTGCTATATATCTGCCGCCCTAGTTGTTAATTTTAGTTTTATTCTGTAGCATGATGCTGCTTGCCGCGTGATTGCAAGGCTTAAAAAGGAACGAGACGAAGCTAGGTCTATACTTGCACAGGCAGAAAGGCAGTTCCCTATTTCTACTCCAAATTCTGTGACAGCTAATGCTCCTGTTCATAGCAATGGGAAAAGAGGTATTATATCTTATCATCTTGTTGAGGTTGAAGTGAATTTCAATAAAATTTCGTACCCCCTAAACATTAGAGTTTAAAGTGTTCAAAAACTAAGAGTTTGCACCTTAATAGCTTCCCCTATGTGCTGTTAATTCTTGCGCTTTGCACATTTGAATTGTCTTTTTTATGTTTTAGCTGCTGATGACGAGGAGTTGGCACCTAATGCGAAGAAATTACATCCTGGAATATCTTCCAGTATAATTTCTGAGCTAACAGACTGCAATGCTGCTCTTTCCGGGAAGAGGAAAAAGCGAGAGGTATTCTTCATTATAGTATAGATTATAGAAGTGTATCAAGTTTCGCAATGGTAGTATTTTCTTAGTTTGCTTAGATCCTTTGAAAAATTTGAAATTACACACACACACACACACATTAATGGCATTTACATTTAACTTGTTTGTGACATGTTCTATGGTTTATTTCAGATTCCTGCGACTTTGGCTTCTGTTGACGCTCTAGAGACATATACGCAGATATCTAGCCATCCCTTAAACAAAACTAGCAAACAGGGCATTATAGCTCTTGATATCCTTCATTCTAAGGTATTACTTTCAATCTCTAAGTAATATTCAAAAGCAAAATATTTTTTAATTAAGGCATTATAGCTCTTGATATCCTTTATTCTAAGGTATTACTTTTAATCTCTACAAGTAATATTCAAAAACGACATATTTTTTAATTAGAAAACTAATGTGCAATGACTGAGGATCAACTTATTCCGTTTTACAGGACATAATCGCAACTGGAAATATTGATACAAATGCTGTTATTTTTGACCGTCCATCAGGACAAGTATTAGCTACACTCAGCGGTCACTCTAAAAAGGTCGGTCTTATTTTAGTTGGAATTGAAGGATTTATATTTTCTTCAAAATCTATTAGCTGACCTGTAATGCTTTTGGTAGGTAACAAGCGTGAAGTTTGTAGGTCAGGGTGAATCTATCATAACTGGTTCAGCAGATAAGGTAAATAGCTATCCCTTTCTAATTTTATAAGGTAGTTTGGTTTCACAGCTCAGAAGTTGGAATTATTTTATCTGATCACCCTCTTTCGTCTTAGAGCAGACATTGTAAAGCTTGATACAAATTTCAATCCATGTATTGCTACATTCCTTGTTTTCTCCAGACACAACCAATTGTGTTTTGCATGATGCAGCTAATCCAGACCTGTGAATTTATATGTGAACTATGGAATCCAAAATTCACACTTGATATTTTTCAGTCCCTTGCGATTTACATTTGCATTTTGTTCCCCATAATAGATGTGTCTGTTGAATGTCATTCTCACTTGGCCGTCTTAAAATTGTGAATTTGAGTTACCCTGAAAAGATGTAGTTTTGCATTCCCTAATGGTCTATAAATCTTTTAATTTATTTGTATTGCAGACAGTTCGTTTGTGGCAAGGGTCAGATGATGGTCACTATAACTGCAAACAGATCTTGAAGGATCATTCTGCTGAAGTAAGTGAATCTAACCACATTGCTGTTCCTAATTTTTCATTGTTTCTTGTTTGTCTTTATCCTACTTGTAATTCGGTATGAAATTGAATCTTGGGCGACAAGAAACATTAAGAAAATAAACTCGTAGAGAGATGATAGTTATGGCGTTGTTTGAGCCACATGTAGTTAACCCCACCTAGTACAAGTAGGATAAAACTTGATTGTCTTTTTGGGGTAATTGAATGATATAGATTCTTTTGTAAAATAATAAATGTTTTGGAGATAATACATGTTCTTCAAATTATAACACAACACCTCAAGAATCCAAGAGGTGAAGTCCTCAAAAAGGAAACTTTTCATTTTCACTTCCCCTGGTTCTCTGAACCGTTAACCTCCCACTTCCTCATTCCTCCTTAACTCCTTAACATTATGATAACAGGCTTATTCAGGTCCCTACTCTGTTGCTCCACAGTTAACTTTTACAAATCTGCAATTCTTTCTTGAAAATAATTTTTACTTCATAAAGATTTAGAGCTACCACCTTAGTCAGAGATATCTCCGCACATTGAGTTCGATCACATGAACAGTCGTAGATTATGTCGTGGGATGTCAGTCGGGACTAATGAGTATCCTTGGATTGTGGCAAAAGCTATTTCTCATAGCCTATGCCAATTAGACCACTTACCATATTGACCTTGACATGTTGGCCTGTGAAGTCCTTGTTGAAAGCCAGCATTCTCACAAACAGCTCTTAACAGACTTTAAAACATTTTTGGTTGTGGATGGGGATCTCTCATAGCCATGTTTTAGCAAAAGTTTATTTTGGGAGATGCCAATGCAAAACAAACATGCACTTAATTATGTGTTCTGTATTTTGTACATAAGAATGCATACACCATATATTTCAATTAAATTAGTTAGAATCTGACGTATGCACTTTGAATTAGCAACATCCTCTTTACCCTGGTCAAATAGTTGCGTACACAGTCACCCGCTCACATCAAATGCATGCCATTCTGTACCAGATAAGAAGTTCTCAATCCTGAATCAGAATTTGAACGATTCATGCAAAAGCAGCTATAACATGCTCTATCCCGACAGTTTTATTTTAAATGCAGAAAAGAATGTAGACATCTGAAAGAAACCCATTAACTCTTGACTGCTAGGTTTTGGTATACAAATTATTGGGAAAAACCCAAGTCCTAGATTGGATAGATCTCTTGATAAGAATTTATAAATAGAGGCACTCCTCACCTTACAAGCCAGTCTTGCAAGGATGAGTTAGGCCCAATTTAAATTAAATTTGGTGGTTCTATCACTTCTACAACTGATGCATGACCTCTTTAGTAAGTCGTACATCCAGTCGAGTACCTTCACTACAATTTGATGTTTTCTAGTTTGATATTCAGCACTCATGTTATTTCTTTTTTAAAAAAAACTTGAGGTATAAAACACTATCTTTATCTTCAATCCAAGAATCAGGATTTCATAAACTATGAATGCCTTTATGATTGCTATTCTTGACTATCTGAGGGTGAAACTTTCAAATCTATGAATTTATGCATCCTTATTTCCCGCCAGTTATGTTTCTCTCATACACATATATTCTGTTGTGATGTAAGAACAAATTTGCCTTTCCAACTTTTCAGGTGGAAGCTGTCACTGTCCATGCCACCAATAATTACTTTGTGACTGCATCACTTGATGGCAGCTGGTGCTTTTATGAACTATCTTCAGGAACTTGTCTGACTCAGGTCCGAACATTTTTTCTTTTATATTTGTGTTAATATTGATTCATAAGTGTCTTGTGGAATAGTTTTGTAGAGGTGTCTAAAAGAATACGGGGTATTATTATTATTATTATTATTACTACTATTATTTTTTATTTTTTATATTTCTTGATAAGTCATCTGAAAGCAACAACTTTGAACCTTTACTGCATTCCTTCCTACCTAAGTGCTGAGTTGACAATGATTTGGCATTTATAGGTTTCTGACTCTTCCGAAGGCTATACATCTGCAGCTTTTCATCCCGATGGTCTTATTCTTGGAACTGGCACTACAGATTCTCTTGTTAAGATCTGGGATGTAAAAAGTCAGGTATGCAATATATGTTTGCTTTATTCCTAGATTAATTTCAATATTAATTTCTATTTTAATATTAATTTTATTTTGAAATGATATTTGGTTGCAGGCAAATGTTGCTAAGTTTGATGGACATGTCGGGCATGTAACTGCAATATCCTTTTCTGAGAATGGATACTACCTTGCGGTACGTGAAAAAAATGTAACCAGTATTAGCAGGTTTATCATTAATATGGTATTAACGTCAAAAAAAAAAAATCATTAATATGGTATTCTTATCGTACTCTTGAATTTGTGATAGACCGCAGCTCATGATGGTGTAAAGCTTTGGGATCTACGCAAGTTGAAAAACTTTCGGGACTATGGCCCATATGACTCAGCAACTCCAACAAACTCTGGTATTGACTCTCTCTTATTGTCAATGGCCCTACTATTTACATACTAGAATATTGTACCTATTTCATAAGGTCTTCATCTAATTCTTTGTATCTTGCAGTGGAGTTTGACCACAGTGGATCCTACCTTGCAGTTGCAGGCTCTGATGTTAGGTGAGGAGTAATGTCGGGAAATACTTGTGTGACATTAATGTCACGGTGACATTAAATCTGGGCCACACATTGGATGACATTAGATGTGTTGTGTGGTCCAGATACAATGACAATGACAATGTATGGTCCAGATGCAATGGCACGGTGACATTAATGTCACCAAAGTGCCACCCCTTGATATTCTATAAGCTGTTTTCATTTTCAGTTTATTTTCATTAGTTTAACGACACGATAGTTCATAGAAGTTGTTTTTGCATAGCTTAGGGGAAGATTACAATGTTTTATTTTTTAATTATTATATTGAAAAAAGTTGTACATAAACTCTTCTATGATAATTGTTTACTTTTATATGATAATAATGCATTGATCAAATGTTTACCCAAATACCCTTTAGATTGGTAATGATTTAAAAATGTGACCGAAGAATTATATTGATTTTTGGTTTCTCTAACCATTCCATCAGTCTTTCCTTGAACTAATTTATTGTACCTGCTACTTTATGCAGGATATTACAAGTTGCAAATGTGAAATCTGAATGGAACTTGATCAAAACTCTTCCTGATTTATCTGGAACAGGTACCGTCTTGATTTTTCCTGCTCCTATCTATCTATTATTATTCCTGCACCATACACATCTAGTATTTATTGTTTGTACAGTTATTAAGATGCAACTTGAACCATTTATTTTCAGGTAAATCCACATGTGTAAAATTCGGCCCAGATTCAGAATACTTAGCAGTTGCATCAATGGACCGGAATCTTCGAATATTTGGCCAACCTGGCGAAGATATTTCTGACAAATCATGA
Protein sequence

>MS.gene073888.t1

DFGKCPVTGEPLTLDDIVPIKTGKIVKPRPVQAASIPGMLGMFQNEWDGLMLSNFALEQQLHTARQELSHALYQHDAACRVIARLKKERDEARSILAQAERQFPISTPNSVTANAPVHSNGKRAADDEELAPNAKKLHPGISSSIISELTDCNAALSGKRKKREIPATLASVDALETYTQISSHPLNKTSKQGIIALDILHSKDIIATGNIDTNAVIFDRPSGQVLATLSGHSKKVTSVKFVGQGESIITGSADKTVRLWQGSDDGHYNCKQILKDHSAEVEAVTVHATNNYFVTASLDGSWCFYELSSGTCLTQVSDSSEGYTSAAFHPDGLILGTGTTDSLVKIWDVKSQANVAKFDGHVGHVTAISFSENGYYLATAAHDGVKLWDLRKLKNFRDYGPYDSATPTNSVEFDHSGSYLAVAGSDVRILQVANVKSEWNLIKTLPDLSGTGKSTCVKFGPDSEYLAVASMDRNLRIFGQPGEDISDKS