AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32424

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32424.t1 MTR_1g026830 96.447 591 21 0 1 591 1 591 0.0 1170
MS.gene32424.t1 MTR_1g095810 94.128 579 33 1 14 591 11 589 0.0 1117
MS.gene32424.t1 MTR_2g100230 40.191 418 237 5 117 531 66 473 4.82e-99 310
MS.gene32424.t1 MTR_3g083690 38.741 413 227 6 136 531 134 537 8.76e-93 298
MS.gene32424.t1 MTR_4g105100 40.898 401 224 5 134 531 189 579 2.09e-92 297
MS.gene32424.t1 MTR_3g467550 37.624 404 230 5 111 500 77 472 3.53e-92 295
MS.gene32424.t1 MTR_6g005550 39.416 411 231 7 126 531 144 541 6.68e-91 295
MS.gene32424.t1 MTR_1g054630 39.312 407 226 6 136 528 143 542 8.65e-91 292
MS.gene32424.t1 MTR_7g109720 43.544 395 208 7 117 506 202 586 1.39e-90 296
MS.gene32424.t1 MTR_3g467550 37.500 400 205 6 111 500 77 441 8.62e-88 282
MS.gene32424.t1 MTR_4g134170 35.748 428 250 4 120 529 260 680 3.32e-86 283
MS.gene32424.t1 MTR_4g114120 38.049 410 230 6 138 530 131 533 3.39e-85 279
MS.gene32424.t1 MTR_6g005320 35.648 432 251 5 116 529 218 640 2.04e-84 277
MS.gene32424.t1 MTR_2g032630 39.806 412 230 7 125 531 474 872 8.37e-83 281
MS.gene32424.t1 MTR_2g007000 38.626 422 240 6 117 531 474 883 9.49e-82 278
MS.gene32424.t1 MTR_3g467550 35.620 379 222 5 111 475 77 447 8.00e-78 254
MS.gene32424.t1 MTR_5g027840 37.468 395 230 8 138 525 95 479 1.80e-76 252
MS.gene32424.t1 MTR_4g105100 39.879 331 187 4 134 461 189 510 9.19e-70 234
MS.gene32424.t1 MTR_4g079320 33.744 406 245 7 148 543 20 411 1.38e-63 223
MS.gene32424.t1 MTR_5g090120 37.500 360 202 8 157 508 91 435 6.74e-63 218
MS.gene32424.t1 MTR_6g082080 33.243 370 223 6 145 507 8 360 5.34e-62 211
MS.gene32424.t1 MTR_7g056830 33.243 370 223 6 145 507 8 360 8.45e-62 211
MS.gene32424.t1 MTR_3g449250 36.592 358 204 8 157 506 100 442 1.30e-61 213
MS.gene32424.t1 MTR_3g449250 36.592 358 204 8 157 506 100 442 2.30e-61 213
MS.gene32424.t1 MTR_6g033070 31.308 428 277 9 125 548 119 533 2.66e-61 212
MS.gene32424.t1 MTR_3g049990 35.616 365 215 6 147 504 152 503 2.24e-60 214
MS.gene32424.t1 MTR_7g100820 32.530 415 251 8 127 531 110 505 1.15e-58 207
MS.gene32424.t1 MTR_2g041140 34.349 361 222 4 147 502 141 491 1.63e-57 206
MS.gene32424.t1 MTR_2g039960 31.105 389 249 8 147 532 41 413 5.12e-56 194
MS.gene32424.t1 MTR_2g066050 33.333 369 217 7 147 504 53 403 8.84e-56 197
MS.gene32424.t1 MTR_4g125970 31.105 389 249 8 147 532 41 413 1.15e-55 194
MS.gene32424.t1 MTR_2g438110 31.378 392 250 7 144 532 31 406 2.25e-55 192
MS.gene32424.t1 MTR_4g125970 31.105 389 249 8 147 532 58 430 2.27e-55 193
MS.gene32424.t1 MTR_2g099620 30.513 390 252 8 146 532 24 397 9.10e-54 188
MS.gene32424.t1 MTR_2g099620 30.513 390 252 8 146 532 40 413 1.08e-53 188
MS.gene32424.t1 MTR_8g018400 29.820 389 254 8 147 532 41 413 6.11e-53 186
MS.gene32424.t1 MTR_4g112340 35.030 334 198 8 165 490 115 437 2.34e-52 189
MS.gene32424.t1 MTR_2g055850 30.769 364 237 6 144 501 88 442 1.41e-51 186
MS.gene32424.t1 MTR_3g071620 32.275 378 224 11 148 501 15 384 6.71e-51 185
MS.gene32424.t1 MTR_7g076820 28.306 431 279 7 104 532 118 520 1.23e-50 183
MS.gene32424.t1 MTR_7g076800 29.975 397 258 7 139 532 132 511 3.98e-50 181
MS.gene32424.t1 MTR_2g043360 28.365 416 218 8 165 507 60 468 4.55e-50 183
MS.gene32424.t1 MTR_4g059460 29.268 410 210 9 165 501 63 465 5.04e-50 183
MS.gene32424.t1 MTR_7g076830 28.211 436 273 8 104 532 111 513 5.77e-50 181
MS.gene32424.t1 MTR_6g056080 33.333 369 222 9 139 497 98 452 1.14e-49 183
MS.gene32424.t1 MTR_8g027780 34.239 368 214 9 148 495 102 461 4.54e-49 179
MS.gene32424.t1 MTR_1g102410 33.333 390 204 10 142 497 42 409 6.28e-49 180
MS.gene32424.t1 MTR_8g027775 32.642 386 227 11 135 495 80 457 7.37e-48 176
MS.gene32424.t1 MTR_4g010565 29.948 384 238 10 148 516 125 492 2.18e-46 171
MS.gene32424.t1 MTR_3g107420 30.084 359 234 7 148 502 48 393 2.73e-45 166
MS.gene32424.t1 MTR_1g012420 29.787 376 242 8 136 502 31 393 8.96e-45 164
MS.gene32424.t1 MTR_2g063080 27.924 419 245 7 139 505 101 514 4.54e-44 165
MS.gene32424.t1 MTR_6g039840 28.836 378 241 8 144 503 107 474 6.44e-43 164
MS.gene32424.t1 MTR_8g027770 33.240 358 206 9 158 495 212 556 3.74e-42 161
MS.gene32424.t1 MTR_8g097000 32.778 360 217 10 158 501 299 649 4.35e-41 159
MS.gene32424.t1 MTR_1g061970 30.536 429 236 17 136 523 68 475 5.54e-41 155
MS.gene32424.t1 MTR_5g092560 32.184 348 219 8 166 506 95 432 7.22e-41 158
MS.gene32424.t1 MTR_7g057250 27.902 448 245 12 148 525 164 603 1.67e-39 154
MS.gene32424.t1 MTR_8g027780 32.653 343 203 9 148 470 102 436 1.25e-38 148
MS.gene32424.t1 MTR_1g083130 27.295 414 236 9 146 508 25 424 1.21e-36 145
MS.gene32424.t1 MTR_4g068990 30.295 373 239 11 143 507 73 432 1.47e-36 145
MS.gene32424.t1 MTR_4g112320 34.454 238 145 6 261 490 21 255 2.12e-34 135
MS.gene32424.t1 MTR_3g118120 26.804 388 243 11 144 501 129 505 3.48e-34 137
MS.gene32424.t1 MTR_2g043360 27.640 322 160 6 259 507 29 350 1.75e-33 134
MS.gene32424.t1 MTR_4g049910 29.474 380 215 10 148 501 56 408 4.59e-33 132
MS.gene32424.t1 MTR_0915s0010 29.693 293 188 5 164 454 6 282 1.69e-32 127
MS.gene32424.t1 MTR_4g131750 27.586 435 245 14 139 532 74 479 7.89e-30 123
MS.gene32424.t1 MTR_6g039840 28.859 298 185 7 223 503 11 298 6.95e-29 120
MS.gene32424.t1 MTR_1g046570 29.137 278 181 8 266 532 126 398 1.04e-28 119
MS.gene32424.t1 MTR_5g069330 25.000 364 251 10 145 502 1 348 4.43e-28 117
MS.gene32424.t1 MTR_5g069330 25.000 364 251 10 145 502 5 352 4.71e-28 117
MS.gene32424.t1 MTR_4g459350 32.512 203 111 4 165 348 60 255 5.94e-26 108
MS.gene32424.t1 MTR_4g077370 35.238 210 109 7 147 348 250 440 1.93e-24 107
MS.gene32424.t1 MTR_4g077370 32.227 211 99 6 147 348 2 177 6.36e-19 90.1
MS.gene32424.t1 MTR_8g018370 23.823 361 231 7 177 532 3 324 2.43e-24 104
MS.gene32424.t1 MTR_5g092560 30.918 207 137 4 306 506 15 221 5.20e-23 103
MS.gene32424.t1 MTR_8g042690 25.143 350 223 10 170 501 41 369 1.23e-21 98.6
MS.gene32424.t1 MTR_4g068990 30.288 208 138 5 306 507 15 221 1.85e-20 95.5
MS.gene32424.t1 MTR_5g077690 26.178 382 215 12 184 505 70 444 2.85e-20 94.7
MS.gene32424.t1 MTR_3g107420 28.708 209 134 5 146 352 46 241 4.16e-20 92.0
MS.gene32424.t1 MTR_8g032230 35.366 164 92 4 145 307 8 158 2.25e-19 86.3
MS.gene32424.t1 MTR_1g022305 25.439 342 177 11 169 506 26 293 4.06e-19 90.9
MS.gene32424.t1 MTR_3g063040 24.507 355 230 11 155 498 2 329 2.95e-13 73.6
MS.gene32424.t1 MTR_8g062720 25.248 202 133 4 322 507 345 544 9.23e-12 68.2
MS.gene32424.t1 MTR_8g080160 31.356 118 76 1 390 502 480 597 1.96e-11 67.8
MS.gene32424.t1 MTR_0427s0010 28.079 203 109 6 316 501 1 183 4.06e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32424.t1 AT5G51280 82.601 592 101 2 1 591 1 591 0.0 1010
MS.gene32424.t1 AT4G33370 81.092 513 97 0 79 591 30 542 0.0 874
MS.gene32424.t1 AT1G55150 39.518 415 238 5 120 531 73 477 1.86e-99 311
MS.gene32424.t1 AT1G55150 39.518 415 238 5 120 531 98 502 1.31e-98 310
MS.gene32424.t1 AT5G63120 40.952 420 233 7 117 532 136 544 1.39e-96 307
MS.gene32424.t1 AT3G58510 38.519 405 230 6 136 528 141 538 6.58e-93 298
MS.gene32424.t1 AT3G58510 38.519 405 230 6 136 528 141 538 6.58e-93 298
MS.gene32424.t1 AT3G58510 38.519 405 230 6 136 528 141 538 6.58e-93 298
MS.gene32424.t1 AT2G47330 40.798 451 250 8 87 531 168 607 1.52e-92 301
MS.gene32424.t1 AT3G58570 38.875 409 229 7 134 528 134 535 3.23e-92 297
MS.gene32424.t1 AT2G42520 37.653 409 234 6 134 528 147 548 4.03e-91 294
MS.gene32424.t1 AT2G42520 37.653 409 234 6 134 528 147 548 4.03e-91 294
MS.gene32424.t1 AT2G42520 37.653 409 234 6 134 528 147 548 4.03e-91 294
MS.gene32424.t1 AT2G33730 33.835 532 293 12 39 529 194 707 3.79e-88 288
MS.gene32424.t1 AT1G20920 37.678 422 248 5 117 533 162 573 1.62e-87 289
MS.gene32424.t1 AT1G20920 37.915 422 247 5 117 533 500 911 1.03e-85 290
MS.gene32424.t1 AT1G20920 37.915 422 247 5 117 533 500 911 1.03e-85 290
MS.gene32424.t1 AT1G20920 37.915 422 247 5 117 533 500 911 1.03e-85 290
MS.gene32424.t1 AT1G20920 37.915 422 247 5 117 533 500 911 1.03e-85 290
MS.gene32424.t1 AT1G20920 37.915 422 247 5 117 533 500 911 1.03e-85 290
MS.gene32424.t1 AT1G20920 37.915 422 247 5 117 533 500 911 1.03e-85 290
MS.gene32424.t1 AT3G01540 38.741 413 231 8 126 531 138 535 1.58e-85 279
MS.gene32424.t1 AT3G01540 38.741 413 231 8 126 531 138 535 1.74e-85 278
MS.gene32424.t1 AT3G01540 38.741 413 231 8 126 531 138 535 1.74e-85 278
MS.gene32424.t1 AT3G01540 38.741 413 231 8 126 531 138 535 1.74e-85 278
MS.gene32424.t1 AT5G14610 38.386 409 230 8 130 531 145 538 5.10e-84 275
MS.gene32424.t1 AT5G14610 38.386 409 230 8 130 531 145 538 5.73e-84 275
MS.gene32424.t1 AT5G14610 38.386 409 230 8 130 531 153 546 9.80e-84 275
MS.gene32424.t1 AT3G06480 39.320 412 232 7 125 531 414 812 1.56e-79 271
MS.gene32424.t1 AT1G31970 37.981 416 222 12 129 525 95 493 2.40e-79 260
MS.gene32424.t1 AT5G14610 38.889 378 211 7 160 531 44 407 2.99e-78 256
MS.gene32424.t1 AT5G14610 38.889 378 211 7 160 531 44 407 2.99e-78 256
MS.gene32424.t1 AT5G14610 37.831 415 232 10 127 531 208 606 4.21e-78 261
MS.gene32424.t1 AT3G09620 36.211 417 216 7 117 525 367 741 4.56e-73 253
MS.gene32424.t1 AT3G09620 36.211 417 216 7 117 525 368 742 4.56e-73 253
MS.gene32424.t1 AT5G63120 39.828 349 196 6 117 461 136 474 1.04e-72 241
MS.gene32424.t1 AT5G63120 39.828 349 196 6 117 461 136 474 1.46e-72 241
MS.gene32424.t1 AT5G63120 39.828 349 196 6 117 461 136 474 1.46e-72 241
MS.gene32424.t1 AT5G63120 39.828 349 196 6 117 461 136 474 1.46e-72 241
MS.gene32424.t1 AT1G28180 37.845 362 202 7 176 529 3 349 8.23e-68 224
MS.gene32424.t1 AT5G60990 33.062 369 235 4 145 507 8 370 3.54e-64 218
MS.gene32424.t1 AT1G77030 34.010 394 242 6 148 537 30 409 4.50e-64 226
MS.gene32424.t1 AT1G16280 33.793 435 252 11 147 568 59 470 5.19e-64 218
MS.gene32424.t1 AT5G14610 35.475 358 212 7 130 481 153 497 4.22e-63 216
MS.gene32424.t1 AT3G02065 30.695 417 275 7 121 531 83 491 3.21e-61 211
MS.gene32424.t1 AT3G02065 30.695 417 275 7 121 531 83 491 3.21e-61 211
MS.gene32424.t1 AT3G06480 37.714 350 201 6 126 470 415 752 1.05e-60 215
MS.gene32424.t1 AT3G09720 34.063 411 237 11 101 500 96 483 1.16e-60 211
MS.gene32424.t1 AT3G09720 34.063 411 237 11 101 500 96 483 1.16e-60 211
MS.gene32424.t1 AT3G22330 35.311 354 214 7 157 506 115 457 1.44e-60 212
MS.gene32424.t1 AT4G16630 33.251 406 242 7 147 538 168 558 2.95e-60 214
MS.gene32424.t1 AT3G22310 34.986 363 221 7 150 508 120 471 8.11e-59 207
MS.gene32424.t1 AT3G22310 34.986 363 221 7 150 508 120 471 1.06e-58 207
MS.gene32424.t1 AT5G26742 32.203 413 244 7 151 547 107 499 1.15e-58 209
MS.gene32424.t1 AT5G26742 32.203 413 244 7 151 547 107 499 1.16e-58 209
MS.gene32424.t1 AT3G13920 29.426 418 273 9 118 532 14 412 9.37e-58 199
MS.gene32424.t1 AT3G13920 30.513 390 252 8 146 532 29 402 1.96e-57 198
MS.gene32424.t1 AT3G13920 29.426 418 268 10 118 532 14 407 4.98e-56 194
MS.gene32424.t1 AT5G26742 32.637 383 222 7 181 547 44 406 6.15e-56 200
MS.gene32424.t1 AT1G51380 30.691 391 249 6 145 532 21 392 1.99e-55 192
MS.gene32424.t1 AT3G13920 29.056 413 272 8 118 527 14 408 2.09e-55 193
MS.gene32424.t1 AT3G13920 29.056 413 272 8 118 527 18 412 3.07e-55 193
MS.gene32424.t1 AT1G72730 29.820 389 254 8 147 532 42 414 5.21e-55 192
MS.gene32424.t1 AT5G65900 32.578 353 217 7 146 490 154 493 1.21e-54 196
MS.gene32424.t1 AT3G19760 30.534 393 250 7 145 532 34 408 1.78e-54 191
MS.gene32424.t1 AT1G71370 32.368 380 235 9 141 507 9 379 3.92e-54 193
MS.gene32424.t1 AT3G18600 32.320 362 230 6 133 490 77 427 8.01e-54 192
MS.gene32424.t1 AT1G54270 30.612 392 249 8 146 532 39 412 8.34e-54 189
MS.gene32424.t1 AT1G54270 31.283 374 234 8 164 532 52 407 1.12e-52 186
MS.gene32424.t1 AT3G02065 30.387 362 240 6 174 531 1 354 3.82e-52 183
MS.gene32424.t1 AT5G62190 32.115 383 236 9 140 512 93 461 3.63e-51 187
MS.gene32424.t1 AT5G05450 31.510 384 225 10 146 501 9 382 2.20e-50 184
MS.gene32424.t1 AT4G00660 29.293 396 262 6 139 532 123 502 2.63e-49 179
MS.gene32424.t1 AT4G00660 29.293 396 262 6 139 532 123 502 2.63e-49 179
MS.gene32424.t1 AT2G45810 30.685 365 236 5 139 501 146 495 3.56e-49 179
MS.gene32424.t1 AT3G61240 30.618 356 229 6 148 501 126 465 4.13e-49 178
MS.gene32424.t1 AT3G61240 30.618 356 229 6 148 501 126 465 4.13e-49 178
MS.gene32424.t1 AT2G40700 29.710 414 207 9 165 502 50 455 9.78e-48 176
MS.gene32424.t1 AT1G12770 27.952 415 239 8 144 505 108 515 7.36e-47 173
MS.gene32424.t1 AT1G12770 27.952 415 239 8 144 505 108 515 7.36e-47 173
MS.gene32424.t1 AT5G11170 30.000 380 234 11 136 501 31 392 5.32e-46 168
MS.gene32424.t1 AT5G11200 30.000 380 234 11 136 501 31 392 5.32e-46 168
MS.gene32424.t1 AT5G54910 30.856 444 257 14 144 562 69 487 9.12e-44 167
MS.gene32424.t1 AT5G63630 32.102 352 218 7 158 495 66 410 1.44e-43 163
MS.gene32424.t1 AT5G63630 32.102 352 218 7 158 495 260 604 1.34e-42 163
MS.gene32424.t1 AT4G09730 28.646 384 232 10 145 503 111 477 4.24e-42 160
MS.gene32424.t1 AT5G11170 31.288 326 197 10 185 501 2 309 6.18e-41 152
MS.gene32424.t1 AT2G07750 30.713 407 256 9 113 501 340 738 9.13e-41 159
MS.gene32424.t1 AT5G08610 31.461 356 215 7 158 495 394 738 1.11e-40 158
MS.gene32424.t1 AT5G11200 27.962 422 229 13 136 501 31 433 1.53e-40 154
MS.gene32424.t1 AT1G63250 31.054 351 224 7 158 495 340 685 1.83e-39 154
MS.gene32424.t1 AT5G08620 30.899 356 217 8 158 495 92 436 3.08e-39 152
MS.gene32424.t1 AT5G11200 27.231 437 231 13 136 501 31 451 3.72e-39 150
MS.gene32424.t1 AT3G53110 29.351 385 229 13 135 495 80 445 4.36e-38 147
MS.gene32424.t1 AT1G71280 30.240 334 192 10 139 465 11 310 2.22e-36 139
MS.gene32424.t1 AT1G71280 29.882 338 196 10 139 469 11 314 9.46e-36 140
MS.gene32424.t1 AT3G16840 27.273 429 233 11 148 506 193 612 2.08e-33 136
MS.gene32424.t1 AT3G16840 27.273 429 233 11 148 506 192 611 2.49e-33 136
MS.gene32424.t1 AT3G06980 28.721 383 237 13 146 501 374 747 2.70e-33 136
MS.gene32424.t1 AT4G34910 28.321 399 223 11 144 491 44 430 4.31e-32 131
MS.gene32424.t1 AT5G19210 26.632 383 239 10 136 490 66 434 2.42e-29 122
MS.gene32424.t1 AT1G51380 29.151 271 174 5 145 412 21 276 1.37e-27 112
MS.gene32424.t1 AT1G51380 30.233 215 136 2 145 359 21 221 3.89e-25 106
MS.gene32424.t1 AT1G51380 30.233 215 136 2 145 359 21 221 6.19e-25 105
MS.gene32424.t1 AT4G15850 24.455 413 236 10 159 505 44 446 7.30e-22 99.8
MS.gene32424.t1 AT5G19210 27.155 232 147 7 281 490 46 277 1.01e-17 84.7
MS.gene32424.t1 AT5G19210 27.155 232 147 7 281 490 46 277 1.01e-17 84.7
MS.gene32424.t1 AT1G28180 37.795 127 63 3 176 297 3 118 6.80e-15 72.0
MS.gene32424.t1 AT1G27880 24.251 367 217 13 173 515 274 603 5.18e-13 72.8
MS.gene32424.t1 AT1G27880 24.251 367 217 13 173 515 274 603 5.96e-13 72.4

Find 143 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATTATGACGTTCTAATTT+TGG 0.116059 1.4:-12674631 None:intergenic
TTTAGCAAAATTCTGAATTT+TGG 0.136024 1.4:-12673975 None:intergenic
CCATCCAATGGTAGCTAAAA+TGG 0.220873 1.4:+12672935 None:intergenic
GGAATTGCTTTCACGGGTTC+TGG 0.240475 1.4:+12673503 MS.gene32424:CDS
CACATAGTTGTTGCCACGCC+TGG 0.274883 1.4:+12673779 MS.gene32424:CDS
CGTCCCACATTGACAATAAT+AGG 0.278247 1.4:-12674013 None:intergenic
TTGTTACCTTTGAAGGAATC+TGG 0.284937 1.4:+12673677 MS.gene32424:CDS
CTCAGACCTTTGCTTTGTAT+TGG 0.297978 1.4:+12673710 MS.gene32424:CDS
TTTCTAGAATCATCATCTAT+TGG 0.306294 1.4:-12673050 None:intergenic
ACAATTCTTGTTACCTTTGA+AGG 0.309882 1.4:+12673670 MS.gene32424:CDS
GGCGAATCTTGATGTTATTC+AGG 0.315986 1.4:+12674045 MS.gene32424:CDS
AATAATAGGAAGGATTGTTT+TGG 0.320959 1.4:+12674667 MS.gene32424:CDS
GTGTGGCAAATGCAAATAAT+AGG 0.343166 1.4:+12674653 MS.gene32424:CDS
TGTGCAGCCAACACCTATTC+AGG 0.343871 1.4:+12673439 MS.gene32424:CDS
CAAGCAATGTTGTCTCAGTT+TGG 0.345951 1.4:-12674453 None:intergenic
TTAATGACATGTTGAATATC+AGG 0.372122 1.4:-12674331 None:intergenic
TCAATCAGCTCAGCCAAAAC+AGG 0.376407 1.4:-12674517 None:intergenic
AGCCTCCTTTGCATATTAGA+AGG 0.387546 1.4:+12673276 MS.gene32424:CDS
ACCGGAAGATGTGGCAAAAC+TGG 0.393311 1.4:+12674403 MS.gene32424:CDS
AGACCTTTGCTTTGTATTGG+TGG 0.394935 1.4:+12673713 MS.gene32424:CDS
TCCCTCCCAGATAAAATAAC+AGG 0.398029 1.4:-12673473 None:intergenic
TACAGAACAGATTGTTCAAC+AGG 0.398392 1.4:+12673151 MS.gene32424:CDS
GTGGAAGCCGTGGCAATTCA+TGG 0.398757 1.4:+12674205 MS.gene32424:CDS
CTAATTCACGAACCGACATA+AGG 0.404039 1.4:-12673211 None:intergenic
GGAAGCCCTTGCACCTGAAT+AGG 0.407757 1.4:-12673452 None:intergenic
ATCAGGAAAATCCAAGCCTT+TGG 0.408303 1.4:-12674314 None:intergenic
GGCTGATCGATTGGTAGATC+CGG 0.411884 1.4:+12673871 MS.gene32424:CDS
CACGGGTTCTGGCAAAACTT+TGG 0.414563 1.4:+12673514 MS.gene32424:CDS
GAAAATTATGTCCATAGGAT+TGG 0.415181 1.4:+12674376 MS.gene32424:CDS
TATTTGTCCATCGAGGGAAT+TGG 0.416876 1.4:+12673622 MS.gene32424:CDS
ATTGTCAATGTGGGACGTGC+AGG 0.426137 1.4:+12674019 MS.gene32424:CDS
TGCATTGCCATCATGATCAT+TGG 0.427490 1.4:-12673548 None:intergenic
TAATTCACGAACCGACATAA+GGG 0.432495 1.4:-12673210 None:intergenic
TTCTCACAAAATATCAAAAC+AGG 0.435087 1.4:-12674136 None:intergenic
GTCATTTAAGACTGGAAAGA+AGG 0.439351 1.4:+12674264 MS.gene32424:CDS
ATTACGATGCCTGTAGTTCC+CGG 0.440391 1.4:+12673578 MS.gene32424:CDS
AACATCTTCAAAATAGGATC+CGG 0.446469 1.4:-12673401 None:intergenic
AATGGAAGAAGATGATTATG+AGG 0.446862 1.4:+12672953 MS.gene32424:CDS
TATAACGAACCATTGCCTAC+TGG 0.451678 1.4:+12673248 MS.gene32424:CDS
TAAGCCCAGTCTACTTGTTA+AGG 0.455473 1.4:+12673091 MS.gene32424:CDS
ATTTCTTCATTATCTTCCAT+TGG 0.457806 1.4:-12674541 None:intergenic
TTCCGGTACGTCCAATCCTA+TGG 0.458107 1.4:-12674387 None:intergenic
CAAAATATCAAAACAGGAGG+TGG 0.462408 1.4:-12674130 None:intergenic
GCTATTTCGTCATTTAAGAC+TGG 0.467758 1.4:+12674256 MS.gene32424:CDS
GGACAAATAATCAGACCAAA+TGG 0.468369 1.4:-12673608 None:intergenic
TGATATTGCATCCAAAGGCT+TGG 0.472039 1.4:+12674303 MS.gene32424:CDS
AATGATCATGATGGCAATGC+AGG 0.472381 1.4:+12673550 MS.gene32424:CDS
ATAACGAACCATTGCCTACT+GGG 0.472936 1.4:+12673249 MS.gene32424:CDS
GGACCTTCACCGGGAACTAC+AGG 0.475654 1.4:-12673587 None:intergenic
CATGAGTATCTTCTCTTGAA+AGG 0.476814 1.4:+12674181 MS.gene32424:CDS
TTATCTGGGAGGGATATGAT+TGG 0.476899 1.4:+12673482 MS.gene32424:CDS
ATGGCGCGACGCTTCGCCAC+AGG 0.477803 1.4:-12672984 None:intergenic
TATGATTGGAATTGCTTTCA+CGG 0.478341 1.4:+12673496 MS.gene32424:CDS
TTTGAAGGAATCTGGATATC+CGG 0.481624 1.4:+12673685 MS.gene32424:CDS
GAAGAAATCACTGGCATAAG+TGG 0.482770 1.4:+12674556 MS.gene32424:CDS
CAGATAATTTCCCTTTGCGT+TGG 0.485668 1.4:-12673025 None:intergenic
TTGAAGATGTTGAAAACCAA+GGG 0.486333 1.4:+12673413 MS.gene32424:CDS
TGTTGGCTGCACAATCCCCT+TGG 0.486336 1.4:-12673429 None:intergenic
GTTCCCGGTGAAGGTCCATT+TGG 0.490787 1.4:+12673593 MS.gene32424:CDS
CAGACCAAATGGACCTTCAC+CGG 0.492977 1.4:-12673597 None:intergenic
CTTATATCATCTTCAAATCC+CGG 0.494897 1.4:-12673890 None:intergenic
TCCTTAAAATTCTTAATCGC+GGG 0.495673 1.4:-12673368 None:intergenic
AAATGAATCTTGATAACTGC+AGG 0.497521 1.4:+12673831 MS.gene32424:CDS
CAGTGGCATATAATTGTCAA+TGG 0.504194 1.4:+12673332 MS.gene32424:CDS
TGTGGCGAAGCGTCGCGCCA+TGG 0.508155 1.4:+12672986 MS.gene32424:CDS
CACTGGCATAAGTGGTGTCA+AGG 0.508531 1.4:+12674564 MS.gene32424:CDS
TGAGGCCTTAACAAGTAGAC+TGG 0.510721 1.4:-12673096 None:intergenic
ATCCTTAAAATTCTTAATCG+CGG 0.511417 1.4:-12673369 None:intergenic
ACATAGTTGTTGCCACGCCT+GGG 0.514259 1.4:+12673780 MS.gene32424:CDS
ACAGCTTGAGATTGTCAAGA+AGG 0.518951 1.4:+12673751 MS.gene32424:CDS
ATGATTGGAATTGCTTTCAC+GGG 0.521318 1.4:+12673497 MS.gene32424:CDS
CAAAGCAAAGGTCTGAGCTC+CGG 0.523344 1.4:-12673704 None:intergenic
AATTCATGGAGACAAGGATC+AGG 0.523834 1.4:+12674219 MS.gene32424:CDS
GAGAATCTTCTGAGCTTCCA+TGG 0.525892 1.4:-12673003 None:intergenic
TTTGAAGATGTTGAAAACCA+AGG 0.526623 1.4:+12673412 MS.gene32424:CDS
CAGCTTGAGATTGTCAAGAA+GGG 0.528031 1.4:+12673752 MS.gene32424:CDS
AAAATATCAAAACAGGAGGT+GGG 0.530476 1.4:-12674129 None:intergenic
GACTGGAAAGAAGGATGTGT+TGG 0.532068 1.4:+12674273 MS.gene32424:CDS
TTAAAATTCTTAATCGCGGG+TGG 0.532306 1.4:-12673365 None:intergenic
GTTAGATGAGGCTGATCGAT+TGG 0.534300 1.4:+12673862 MS.gene32424:CDS
TTGTCAATGTGGGACGTGCA+GGG 0.535671 1.4:+12674020 MS.gene32424:CDS
CAATGTGGGACGTGCAGGGG+CGG 0.535678 1.4:+12674024 MS.gene32424:CDS
AAATTGAAAATTATGTCCAT+AGG 0.540038 1.4:+12674371 MS.gene32424:CDS
AGAAGATTCTCCAACGCAAA+GGG 0.540048 1.4:+12673015 MS.gene32424:CDS
TGCCACGCCTGGGAGGTTGA+AGG 0.543540 1.4:+12673790 MS.gene32424:CDS
GTCCATAGGATTGGACGTAC+CGG 0.544392 1.4:+12674385 MS.gene32424:CDS
GGCTGAGCTGATTGATCCAA+TGG 0.544866 1.4:+12674525 MS.gene32424:CDS
AACATATCCTTCAACCTCCC+AGG 0.545718 1.4:-12673797 None:intergenic
TGTTCTGTAACACTAATCTC+AGG 0.547023 1.4:-12673137 None:intergenic
GTCATCCTTCTAATATGCAA+AGG 0.548607 1.4:-12673281 None:intergenic
GCTGATCGATTGGTAGATCC+GGG 0.548897 1.4:+12673872 MS.gene32424:CDS
TCTGATTATTTGTCCATCGA+GGG 0.551896 1.4:+12673616 MS.gene32424:CDS
TGCCTCGCCAATTCCCTCGA+TGG 0.556276 1.4:-12673629 None:intergenic
CGGTTCGTGAATTAGCGAAA+GGG 0.557381 1.4:+12673219 MS.gene32424:CDS
GGATATCCAGATTCCTTCAA+AGG 0.559706 1.4:-12673683 None:intergenic
CTTGTCTCCATGAATTGCCA+CGG 0.563651 1.4:-12674212 None:intergenic
GAAGATAATGAAGAAATCAC+TGG 0.564788 1.4:+12674547 MS.gene32424:CDS
GGCTTCCACCCAGTAGGCAA+TGG 0.565220 1.4:-12673257 None:intergenic
ATGCCTGTAGTTCCCGGTGA+AGG 0.572075 1.4:+12673584 MS.gene32424:CDS
GCAACTGATATTGCATCCAA+AGG 0.573465 1.4:+12674298 MS.gene32424:CDS
AAAACCTATTATTGTCAATG+TGG 0.574587 1.4:+12674009 MS.gene32424:CDS
AGACCAAATGGACCTTCACC+GGG 0.576562 1.4:-12673596 None:intergenic
AGCTTGAGATTGTCAAGAAG+GGG 0.576627 1.4:+12673753 MS.gene32424:CDS
TGGGAGGTTGAAGGATATGT+TGG 0.576648 1.4:+12673799 MS.gene32424:CDS
CAGAAGATTCTCCAACGCAA+AGG 0.577959 1.4:+12673014 MS.gene32424:CDS
GTCTGATTATTTGTCCATCG+AGG 0.580654 1.4:+12673615 MS.gene32424:CDS
TGTGCTTCCAATGATCATGA+TGG 0.581752 1.4:+12673541 MS.gene32424:CDS
TTTGGATCTGGAGGTTACAA+AGG 0.585574 1.4:+12674685 MS.gene32424:CDS
CAATCAGCTCAGCCAAAACA+GGG 0.585774 1.4:-12674516 None:intergenic
AAATATCAAAACAGGAGGTG+GGG 0.587549 1.4:-12674128 None:intergenic
TTGTTCTTTCTTTAACTGTG+AGG 0.588178 1.4:-12673114 None:intergenic
GGCAAATGCAAATAATAGGA+AGG 0.588904 1.4:+12674657 MS.gene32424:CDS
TTGATCACTTCAAAGCTCAA+AGG 0.591719 1.4:+12673921 MS.gene32424:CDS
TGTCAATGTGGGACGTGCAG+GGG 0.592163 1.4:+12674021 MS.gene32424:CDS
AAACCTATTATTGTCAATGT+GGG 0.592275 1.4:+12674010 MS.gene32424:CDS
CCAACACCTATTCAGGTGCA+AGG 0.595863 1.4:+12673446 MS.gene32424:CDS
GTCCATCGAGGGAATTGGCG+AGG 0.598686 1.4:+12673627 MS.gene32424:CDS
CAGGTATTTAACGTTAGATG+AGG 0.599810 1.4:+12673850 MS.gene32424:CDS
TCGGTTCGTGAATTAGCGAA+AGG 0.600474 1.4:+12673218 MS.gene32424:CDS
ACTGGCATAAGTGGTGTCAA+GGG 0.604219 1.4:+12674565 MS.gene32424:CDS
CGTGGCAATTCATGGAGACA+AGG 0.604702 1.4:+12674213 MS.gene32424:CDS
ATTGGACGTACCGGAAGATG+TGG 0.605046 1.4:+12674394 MS.gene32424:CDS
ATCCTTCAACCTCCCAGGCG+TGG 0.609061 1.4:-12673792 None:intergenic
CCTTGCACCTGAATAGGTGT+TGG 0.609165 1.4:-12673446 None:intergenic
ATCCTTCTAATATGCAAAGG+AGG 0.612632 1.4:-12673278 None:intergenic
GTATCTTCTCTTGAAAGGAG+TGG 0.613245 1.4:+12674186 MS.gene32424:CDS
AGTAGAGTATGTCAAACAAG+AGG 0.613269 1.4:+12674069 MS.gene32424:CDS
TAGTTGTTGCCACGCCTGGG+AGG 0.621410 1.4:+12673783 MS.gene32424:CDS
CAACACCTATTCAGGTGCAA+GGG 0.626640 1.4:+12673447 MS.gene32424:CDS
ATTAGAACGTCATAATAGTG+TGG 0.631530 1.4:+12674636 MS.gene32424:CDS
ATTCCACCAATACAAAGCAA+AGG 0.634010 1.4:-12673716 None:intergenic
GAGGCCTTAACAAGTAGACT+GGG 0.645952 1.4:-12673095 None:intergenic
TCACAAAATATCAAAACAGG+AGG 0.646136 1.4:-12674133 None:intergenic
ATCAGCTCAGCCAAAACAGG+GGG 0.654999 1.4:-12674514 None:intergenic
GATCATGATGGCAATGCAGG+AGG 0.660735 1.4:+12673553 MS.gene32424:CDS
CTTGAAAGGAGTGGAAGCCG+TGG 0.660852 1.4:+12674195 MS.gene32424:CDS
TGAAGATGTTGAAAACCAAG+GGG 0.665813 1.4:+12673414 MS.gene32424:CDS
GCTTGAGATTGTCAAGAAGG+GGG 0.666059 1.4:+12673754 MS.gene32424:CDS
AAAGGAGGCTTCCACCCAGT+AGG 0.671260 1.4:-12673263 None:intergenic
AATCAGCTCAGCCAAAACAG+GGG 0.693758 1.4:-12674515 None:intergenic
ACGAACCATTGCCTACTGGG+TGG 0.698489 1.4:+12673252 MS.gene32424:CDS
TTATGAGGAGTATATTCCTG+TGG 0.702818 1.4:+12672968 MS.gene32424:CDS
TGATTCTAGAAAACTAAGTG+TGG 0.748043 1.4:+12673061 MS.gene32424:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTGAAAATTATGTCCAT+AGG + chr1.4:12674371-12674390 MS.gene32424:CDS 20.0%
!!! TTAAGAATTTTAAGGATATG+AGG + chr1.4:12673375-12673394 MS.gene32424:CDS 20.0%
!!! TTTAGCAAAATTCTGAATTT+TGG - chr1.4:12673978-12673997 None:intergenic 20.0%
!!! TTTGATATTTTGTGAGAATA+AGG + chr1.4:12674141-12674160 MS.gene32424:CDS 20.0%
! AAAACCTATTATTGTCAATG+TGG + chr1.4:12674009-12674028 MS.gene32424:CDS 25.0%
! AAACCTATTATTGTCAATGT+GGG + chr1.4:12674010-12674029 MS.gene32424:CDS 25.0%
! ATCCTTAAAATTCTTAATCG+CGG - chr1.4:12673372-12673391 None:intergenic 25.0%
! ATTTCTTCATTATCTTCCAT+TGG - chr1.4:12674544-12674563 None:intergenic 25.0%
! TGATAAAAAAACCCTTATGT+CGG + chr1.4:12673199-12673218 MS.gene32424:CDS 25.0%
! TTAATGACATGTTGAATATC+AGG - chr1.4:12674334-12674353 None:intergenic 25.0%
! TTCTCACAAAATATCAAAAC+AGG - chr1.4:12674139-12674158 None:intergenic 25.0%
!! AATAATAGGAAGGATTGTTT+TGG + chr1.4:12674667-12674686 MS.gene32424:CDS 25.0%
!! ACATAATTTTCAATTTCAGC+AGG - chr1.4:12674367-12674386 None:intergenic 25.0%
!! ATCAAGATTCATTTTCTTCT+TGG - chr1.4:12673825-12673844 None:intergenic 25.0%
!! CTATTATGACGTTCTAATTT+TGG - chr1.4:12674634-12674653 None:intergenic 25.0%
!! GTTTTCAACATCTTCAAAAT+AGG - chr1.4:12673410-12673429 None:intergenic 25.0%
!!! TTTCTAGAATCATCATCTAT+TGG - chr1.4:12673053-12673072 None:intergenic 25.0%
AAAATATCAAAACAGGAGGT+GGG - chr1.4:12674132-12674151 None:intergenic 30.0%
AACATCTTCAAAATAGGATC+CGG - chr1.4:12673404-12673423 None:intergenic 30.0%
AATGGAAGAAGATGATTATG+AGG + chr1.4:12672953-12672972 MS.gene32424:CDS 30.0%
ACAATTCTTGTTACCTTTGA+AGG + chr1.4:12673670-12673689 MS.gene32424:CDS 30.0%
ATTAGAACGTCATAATAGTG+TGG + chr1.4:12674636-12674655 MS.gene32424:CDS 30.0%
CTTATATCATCTTCAAATCC+CGG - chr1.4:12673893-12673912 None:intergenic 30.0%
GAAAATTATGTCCATAGGAT+TGG + chr1.4:12674376-12674395 MS.gene32424:CDS 30.0%
GAAGATAATGAAGAAATCAC+TGG + chr1.4:12674547-12674566 MS.gene32424:CDS 30.0%
GTGATTTGATTCAAAAACAG+TGG + chr1.4:12673315-12673334 MS.gene32424:CDS 30.0%
TATGATTGGAATTGCTTTCA+CGG + chr1.4:12673496-12673515 MS.gene32424:CDS 30.0%
TCACAAAATATCAAAACAGG+AGG - chr1.4:12674136-12674155 None:intergenic 30.0%
TCCTTAAAATTCTTAATCGC+GGG - chr1.4:12673371-12673390 None:intergenic 30.0%
TTGAAGATGTTGAAAACCAA+GGG + chr1.4:12673413-12673432 MS.gene32424:CDS 30.0%
TTGTTCTTTCTTTAACTGTG+AGG - chr1.4:12673117-12673136 None:intergenic 30.0%
TTTGAAGATGTTGAAAACCA+AGG + chr1.4:12673412-12673431 MS.gene32424:CDS 30.0%
! AAATGAATCTTGATAACTGC+AGG + chr1.4:12673831-12673850 MS.gene32424:CDS 30.0%
! TGATTCTAGAAAACTAAGTG+TGG + chr1.4:12673061-12673080 MS.gene32424:CDS 30.0%
!! GCAAAATTCTGAATTTTGGT+TGG - chr1.4:12673974-12673993 None:intergenic 30.0%
!! TTTTAAGGATATGAGGTTTC+CGG + chr1.4:12673382-12673401 MS.gene32424:CDS 30.0%
!!! GAATTTTGCTAAAAGTGCTT+TGG + chr1.4:12673985-12674004 MS.gene32424:CDS 30.0%
AAATATCAAAACAGGAGGTG+GGG - chr1.4:12674131-12674150 None:intergenic 35.0%
AGTAGAGTATGTCAAACAAG+AGG + chr1.4:12674069-12674088 MS.gene32424:CDS 35.0%
ATCCTTCTAATATGCAAAGG+AGG - chr1.4:12673281-12673300 None:intergenic 35.0%
ATGATTGGAATTGCTTTCAC+GGG + chr1.4:12673497-12673516 MS.gene32424:CDS 35.0%
ATTCCACCAATACAAAGCAA+AGG - chr1.4:12673719-12673738 None:intergenic 35.0%
CAAAATATCAAAACAGGAGG+TGG - chr1.4:12674133-12674152 None:intergenic 35.0%
CAGGTATTTAACGTTAGATG+AGG + chr1.4:12673850-12673869 MS.gene32424:CDS 35.0%
CAGTGGCATATAATTGTCAA+TGG + chr1.4:12673332-12673351 MS.gene32424:CDS 35.0%
CATGAGTATCTTCTCTTGAA+AGG + chr1.4:12674181-12674200 MS.gene32424:CDS 35.0%
GGACAAATAATCAGACCAAA+TGG - chr1.4:12673611-12673630 None:intergenic 35.0%
GGCAAATGCAAATAATAGGA+AGG + chr1.4:12674657-12674676 MS.gene32424:CDS 35.0%
GTCATCCTTCTAATATGCAA+AGG - chr1.4:12673284-12673303 None:intergenic 35.0%
GTGTGGCAAATGCAAATAAT+AGG + chr1.4:12674653-12674672 MS.gene32424:CDS 35.0%
TAATTCACGAACCGACATAA+GGG - chr1.4:12673213-12673232 None:intergenic 35.0%
TACAGAACAGATTGTTCAAC+AGG + chr1.4:12673151-12673170 MS.gene32424:CDS 35.0%
TCTGATTATTTGTCCATCGA+GGG + chr1.4:12673616-12673635 MS.gene32424:CDS 35.0%
TGAAGATGTTGAAAACCAAG+GGG + chr1.4:12673414-12673433 MS.gene32424:CDS 35.0%
TGTTCTGTAACACTAATCTC+AGG - chr1.4:12673140-12673159 None:intergenic 35.0%
TTAAAATTCTTAATCGCGGG+TGG - chr1.4:12673368-12673387 None:intergenic 35.0%
TTGATCACTTCAAAGCTCAA+AGG + chr1.4:12673921-12673940 MS.gene32424:CDS 35.0%
TTTGAAGGAATCTGGATATC+CGG + chr1.4:12673685-12673704 MS.gene32424:CDS 35.0%
! ACCCGCGATTAAGAATTTTA+AGG + chr1.4:12673367-12673386 MS.gene32424:CDS 35.0%
! GCTATTTCGTCATTTAAGAC+TGG + chr1.4:12674256-12674275 MS.gene32424:CDS 35.0%
! GTCATTTAAGACTGGAAAGA+AGG + chr1.4:12674264-12674283 MS.gene32424:CDS 35.0%
! TCTGTTTTGCTTCTTGCAAA+AGG - chr1.4:12674489-12674508 None:intergenic 35.0%
! TTATGAGGAGTATATTCCTG+TGG + chr1.4:12672968-12672987 MS.gene32424:CDS 35.0%
! TTGTTACCTTTGAAGGAATC+TGG + chr1.4:12673677-12673696 MS.gene32424:CDS 35.0%
! TTTTGCAAGAAGCAAAACAG+AGG + chr1.4:12674488-12674507 MS.gene32424:CDS 35.0%
!!! GGCTTCCTGTTATTTTATCT+GGG + chr1.4:12673468-12673487 MS.gene32424:CDS 35.0%
!!! TCCTGTTATTTTATCTGGGA+GGG + chr1.4:12673472-12673491 MS.gene32424:CDS 35.0%
!!! TTCCTGTTATTTTATCTGGG+AGG + chr1.4:12673471-12673490 MS.gene32424:CDS 35.0%
AATGATCATGATGGCAATGC+AGG + chr1.4:12673550-12673569 MS.gene32424:CDS 40.0%
AATTCATGGAGACAAGGATC+AGG + chr1.4:12674219-12674238 MS.gene32424:CDS 40.0%
ACAGCTTGAGATTGTCAAGA+AGG + chr1.4:12673751-12673770 MS.gene32424:CDS 40.0%
AGAAGATTCTCCAACGCAAA+GGG + chr1.4:12673015-12673034 MS.gene32424:CDS 40.0%
AGACCTTTGCTTTGTATTGG+TGG + chr1.4:12673713-12673732 MS.gene32424:CDS 40.0%
AGCCTCCTTTGCATATTAGA+AGG + chr1.4:12673276-12673295 MS.gene32424:CDS 40.0%
AGCTTGAGATTGTCAAGAAG+GGG + chr1.4:12673753-12673772 MS.gene32424:CDS 40.0%
ATAACGAACCATTGCCTACT+GGG + chr1.4:12673249-12673268 MS.gene32424:CDS 40.0%
ATCAGGAAAATCCAAGCCTT+TGG - chr1.4:12674317-12674336 None:intergenic 40.0%
CAAGCAATGTTGTCTCAGTT+TGG - chr1.4:12674456-12674475 None:intergenic 40.0%
CAGATAATTTCCCTTTGCGT+TGG - chr1.4:12673028-12673047 None:intergenic 40.0%
CAGCTTGAGATTGTCAAGAA+GGG + chr1.4:12673752-12673771 MS.gene32424:CDS 40.0%
CGTCCCACATTGACAATAAT+AGG - chr1.4:12674016-12674035 None:intergenic 40.0%
CTAATTCACGAACCGACATA+AGG - chr1.4:12673214-12673233 None:intergenic 40.0%
CTCAGACCTTTGCTTTGTAT+TGG + chr1.4:12673710-12673729 MS.gene32424:CDS 40.0%
GAAGAAATCACTGGCATAAG+TGG + chr1.4:12674556-12674575 MS.gene32424:CDS 40.0%
GCAACTGATATTGCATCCAA+AGG + chr1.4:12674298-12674317 MS.gene32424:CDS 40.0%
GGATATCCAGATTCCTTCAA+AGG - chr1.4:12673686-12673705 None:intergenic 40.0%
GGCGAATCTTGATGTTATTC+AGG + chr1.4:12674045-12674064 MS.gene32424:CDS 40.0%
GTATCTTCTCTTGAAAGGAG+TGG + chr1.4:12674186-12674205 MS.gene32424:CDS 40.0%
GTCTGATTATTTGTCCATCG+AGG + chr1.4:12673615-12673634 MS.gene32424:CDS 40.0%
TATAACGAACCATTGCCTAC+TGG + chr1.4:12673248-12673267 MS.gene32424:CDS 40.0%
TATTTGTCCATCGAGGGAAT+TGG + chr1.4:12673622-12673641 MS.gene32424:CDS 40.0%
TCCCTCCCAGATAAAATAAC+AGG - chr1.4:12673476-12673495 None:intergenic 40.0%
TGATATTGCATCCAAAGGCT+TGG + chr1.4:12674303-12674322 MS.gene32424:CDS 40.0%
TGCATTGCCATCATGATCAT+TGG - chr1.4:12673551-12673570 None:intergenic 40.0%
TGTGCTTCCAATGATCATGA+TGG + chr1.4:12673541-12673560 MS.gene32424:CDS 40.0%
TTATCTGGGAGGGATATGAT+TGG + chr1.4:12673482-12673501 MS.gene32424:CDS 40.0%
TTTGGATCTGGAGGTTACAA+AGG + chr1.4:12674685-12674704 MS.gene32424:CDS 40.0%
! AAGGGATGTGCATTTTGTTG+CGG + chr1.4:12674583-12674602 MS.gene32424:CDS 40.0%
! TAAGCCCAGTCTACTTGTTA+AGG + chr1.4:12673091-12673110 MS.gene32424:CDS 40.0%
!!! AAGGATTGTTTTGGATCTGG+AGG + chr1.4:12674676-12674695 MS.gene32424:CDS 40.0%
!!! AGGAAGGATTGTTTTGGATC+TGG + chr1.4:12674673-12674692 MS.gene32424:CDS 40.0%
!!! GGGCTTCCTGTTATTTTATC+TGG + chr1.4:12673467-12673486 MS.gene32424:CDS 40.0%
AACATATCCTTCAACCTCCC+AGG - chr1.4:12673800-12673819 None:intergenic 45.0%
AATCAGCTCAGCCAAAACAG+GGG - chr1.4:12674518-12674537 None:intergenic 45.0%
ATTACGATGCCTGTAGTTCC+CGG + chr1.4:12673578-12673597 MS.gene32424:CDS 45.0%
CAACACCTATTCAGGTGCAA+GGG + chr1.4:12673447-12673466 MS.gene32424:CDS 45.0%
CAATCAGCTCAGCCAAAACA+GGG - chr1.4:12674519-12674538 None:intergenic 45.0%
CAGAAGATTCTCCAACGCAA+AGG + chr1.4:12673014-12673033 MS.gene32424:CDS 45.0%
CGGTTCGTGAATTAGCGAAA+GGG + chr1.4:12673219-12673238 MS.gene32424:CDS 45.0%
CTTGTCTCCATGAATTGCCA+CGG - chr1.4:12674215-12674234 None:intergenic 45.0%
GACTGGAAAGAAGGATGTGT+TGG + chr1.4:12674273-12674292 MS.gene32424:CDS 45.0%
GAGAATCTTCTGAGCTTCCA+TGG - chr1.4:12673006-12673025 None:intergenic 45.0%
GAGGCCTTAACAAGTAGACT+GGG - chr1.4:12673098-12673117 None:intergenic 45.0%
GCTTGAGATTGTCAAGAAGG+GGG + chr1.4:12673754-12673773 MS.gene32424:CDS 45.0%
GTTAGATGAGGCTGATCGAT+TGG + chr1.4:12673862-12673881 MS.gene32424:CDS 45.0%
TCAATCAGCTCAGCCAAAAC+AGG - chr1.4:12674520-12674539 None:intergenic 45.0%
TGAGGCCTTAACAAGTAGAC+TGG - chr1.4:12673099-12673118 None:intergenic 45.0%
TGGGAGGTTGAAGGATATGT+TGG + chr1.4:12673799-12673818 MS.gene32424:CDS 45.0%
! ACCAGTTTTGCCACATCTTC+CGG - chr1.4:12674407-12674426 None:intergenic 45.0%
! ACTGGCATAAGTGGTGTCAA+GGG + chr1.4:12674565-12674584 MS.gene32424:CDS 45.0%
! AGGGATGTGCATTTTGTTGC+GGG + chr1.4:12674584-12674603 MS.gene32424:CDS 45.0%
! TCGGTTCGTGAATTAGCGAA+AGG + chr1.4:12673218-12673237 MS.gene32424:CDS 45.0%
! TTTTCTGTAGGCACTCAAGG+AGG - chr1.4:12674108-12674127 None:intergenic 45.0%
ACATAGTTGTTGCCACGCCT+GGG + chr1.4:12673780-12673799 MS.gene32424:CDS 50.0%
ACCGGAAGATGTGGCAAAAC+TGG + chr1.4:12674403-12674422 MS.gene32424:CDS 50.0%
AGACCAAATGGACCTTCACC+GGG - chr1.4:12673599-12673618 None:intergenic 50.0%
ATCAGCTCAGCCAAAACAGG+GGG - chr1.4:12674517-12674536 None:intergenic 50.0%
ATTGGACGTACCGGAAGATG+TGG + chr1.4:12674394-12674413 MS.gene32424:CDS 50.0%
ATTGTCAATGTGGGACGTGC+AGG + chr1.4:12674019-12674038 MS.gene32424:CDS 50.0%
CAAAGCAAAGGTCTGAGCTC+CGG - chr1.4:12673707-12673726 None:intergenic 50.0%
CAGACCAAATGGACCTTCAC+CGG - chr1.4:12673600-12673619 None:intergenic 50.0%
CCAACACCTATTCAGGTGCA+AGG + chr1.4:12673446-12673465 MS.gene32424:CDS 50.0%
CCTTGCACCTGAATAGGTGT+TGG - chr1.4:12673449-12673468 None:intergenic 50.0%
CGTGGCAATTCATGGAGACA+AGG + chr1.4:12674213-12674232 MS.gene32424:CDS 50.0%
GATCATGATGGCAATGCAGG+AGG + chr1.4:12673553-12673572 MS.gene32424:CDS 50.0%
GCTGATCGATTGGTAGATCC+GGG + chr1.4:12673872-12673891 MS.gene32424:CDS 50.0%
GGAATTGCTTTCACGGGTTC+TGG + chr1.4:12673503-12673522 MS.gene32424:CDS 50.0%
GGCTGAGCTGATTGATCCAA+TGG + chr1.4:12674525-12674544 MS.gene32424:CDS 50.0%
GGCTGATCGATTGGTAGATC+CGG + chr1.4:12673871-12673890 MS.gene32424:CDS 50.0%
GTCCATAGGATTGGACGTAC+CGG + chr1.4:12674385-12674404 MS.gene32424:CDS 50.0%
TGTGCAGCCAACACCTATTC+AGG + chr1.4:12673439-12673458 MS.gene32424:CDS 50.0%
TTCCGGTACGTCCAATCCTA+TGG - chr1.4:12674390-12674409 None:intergenic 50.0%
TTGTCAATGTGGGACGTGCA+GGG + chr1.4:12674020-12674039 MS.gene32424:CDS 50.0%
! ACAGAGGATTCCCCCTGTTT+TGG + chr1.4:12674504-12674523 MS.gene32424:CDS 50.0%
! CACGGGTTCTGGCAAAACTT+TGG + chr1.4:12673514-12673533 MS.gene32424:CDS 50.0%
! CACTGGCATAAGTGGTGTCA+AGG + chr1.4:12674564-12674583 MS.gene32424:CDS 50.0%
! GGGTTTTCTGTAGGCACTCA+AGG - chr1.4:12674111-12674130 None:intergenic 50.0%
! TGTGCATTTTGTTGCGGGCT+TGG + chr1.4:12674589-12674608 MS.gene32424:CDS 50.0%
AAAGGAGGCTTCCACCCAGT+AGG - chr1.4:12673266-12673285 None:intergenic 55.0%
ACGAACCATTGCCTACTGGG+TGG + chr1.4:12673252-12673271 MS.gene32424:CDS 55.0%
ATGCCTGTAGTTCCCGGTGA+AGG + chr1.4:12673584-12673603 MS.gene32424:CDS 55.0%
CACATAGTTGTTGCCACGCC+TGG + chr1.4:12673779-12673798 MS.gene32424:CDS 55.0%
CTTGAAAGGAGTGGAAGCCG+TGG + chr1.4:12674195-12674214 MS.gene32424:CDS 55.0%
GGAAGCCCTTGCACCTGAAT+AGG - chr1.4:12673455-12673474 None:intergenic 55.0%
GTGGAAGCCGTGGCAATTCA+TGG + chr1.4:12674205-12674224 MS.gene32424:CDS 55.0%
GTTCCCGGTGAAGGTCCATT+TGG + chr1.4:12673593-12673612 MS.gene32424:CDS 55.0%
TGTCAATGTGGGACGTGCAG+GGG + chr1.4:12674021-12674040 MS.gene32424:CDS 55.0%
! CAGGAGGTGGGGTTTTCTGT+AGG - chr1.4:12674120-12674139 None:intergenic 55.0%
! TGTTGGCTGCACAATCCCCT+TGG - chr1.4:12673432-12673451 None:intergenic 55.0%
ATCCTTCAACCTCCCAGGCG+TGG - chr1.4:12673795-12673814 None:intergenic 60.0%
GGACCTTCACCGGGAACTAC+AGG - chr1.4:12673590-12673609 None:intergenic 60.0%
GGCTTCCACCCAGTAGGCAA+TGG - chr1.4:12673260-12673279 None:intergenic 60.0%
GTCCATCGAGGGAATTGGCG+AGG + chr1.4:12673627-12673646 MS.gene32424:CDS 60.0%
TAGTTGTTGCCACGCCTGGG+AGG + chr1.4:12673783-12673802 MS.gene32424:CDS 60.0%
TGCCTCGCCAATTCCCTCGA+TGG - chr1.4:12673632-12673651 None:intergenic 60.0%
CAATGTGGGACGTGCAGGGG+CGG + chr1.4:12674024-12674043 MS.gene32424:CDS 65.0%
TGCCACGCCTGGGAGGTTGA+AGG + chr1.4:12673790-12673809 MS.gene32424:CDS 65.0%
ATGGCGCGACGCTTCGCCAC+AGG - chr1.4:12672987-12673006 None:intergenic 70.0%
!! TGTGGCGAAGCGTCGCGCCA+TGG + chr1.4:12672986-12673005 MS.gene32424:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 12672942 12674717 12672942 ID=MS.gene32424
chr1.4 mRNA 12672942 12674717 12672942 ID=MS.gene32424.t1;Parent=MS.gene32424
chr1.4 exon 12672942 12674717 12672942 ID=MS.gene32424.t1.exon1;Parent=MS.gene32424.t1
chr1.4 CDS 12672942 12674717 12672942 ID=cds.MS.gene32424.t1;Parent=MS.gene32424.t1
Gene Sequence

>MS.gene32424

ATGGTAGCTAAAATGGAAGAAGATGATTATGAGGAGTATATTCCTGTGGCGAAGCGTCGCGCCATGGAAGCTCAGAAGATTCTCCAACGCAAAGGGAAATTATCTGCTCCAATAGATGATGATTCTAGAAAACTAAGTGTGGTTGAAACTAAGCCCAGTCTACTTGTTAAGGCCTCACAGTTAAAGAAAGAACAACCTGAGATTAGTGTTACAGAACAGATTGTTCAACAGGAGAAAGAGATGATTGAGAATTTGTCTGATAAAAAAACCCTTATGTCGGTTCGTGAATTAGCGAAAGGGATCACTTATAACGAACCATTGCCTACTGGGTGGAAGCCTCCTTTGCATATTAGAAGGATGACAAAGAAAGATTGTGATTTGATTCAAAAACAGTGGCATATAATTGTCAATGGTGAAGATATTCCACCCGCGATTAAGAATTTTAAGGATATGAGGTTTCCGGATCCTATTTTGAAGATGTTGAAAACCAAGGGGATTGTGCAGCCAACACCTATTCAGGTGCAAGGGCTTCCTGTTATTTTATCTGGGAGGGATATGATTGGAATTGCTTTCACGGGTTCTGGCAAAACTTTGGTTTTTGTGCTTCCAATGATCATGATGGCAATGCAGGAGGAAATTACGATGCCTGTAGTTCCCGGTGAAGGTCCATTTGGTCTGATTATTTGTCCATCGAGGGAATTGGCGAGGCAAACTTATGAAGTCATTGAACAATTCTTGTTACCTTTGAAGGAATCTGGATATCCGGAGCTCAGACCTTTGCTTTGTATTGGTGGAATTGATACGAGATCACAGCTTGAGATTGTCAAGAAGGGGGTTCACATAGTTGTTGCCACGCCTGGGAGGTTGAAGGATATGTTGGCCAAGAAGAAAATGAATCTTGATAACTGCAGGTATTTAACGTTAGATGAGGCTGATCGATTGGTAGATCCGGGATTTGAAGATGATATAAGAGAAGTTTTTGATCACTTCAAAGCTCAAAGGCAGACTCTCTTGTTTTCTGCTACTATGCCAACCAAAATTCAGAATTTTGCTAAAAGTGCTTTGGTAAAACCTATTATTGTCAATGTGGGACGTGCAGGGGCGGCGAATCTTGATGTTATTCAGGAAGTAGAGTATGTCAAACAAGAGGCAAAAATTGTTTACCTCCTTGAGTGCCTACAGAAAACCCCACCTCCTGTTTTGATATTTTGTGAGAATAAGGCAGATGTCGATGACATTCATGAGTATCTTCTCTTGAAAGGAGTGGAAGCCGTGGCAATTCATGGAGACAAGGATCAGGAAGATAGAGAGTATGCTATTTCGTCATTTAAGACTGGAAAGAAGGATGTGTTGGTTGCAACTGATATTGCATCCAAAGGCTTGGATTTTCCTGATATTCAACATGTCATTAACTACGACATGCCTGCTGAAATTGAAAATTATGTCCATAGGATTGGACGTACCGGAAGATGTGGCAAAACTGGTATAGCGACAACATTTATAAACAAGAACCAAACTGAGACAACATTGCTTGATTTGAAACACCTTTTGCAAGAAGCAAAACAGAGGATTCCCCCTGTTTTGGCTGAGCTGATTGATCCAATGGAAGATAATGAAGAAATCACTGGCATAAGTGGTGTCAAGGGATGTGCATTTTGTTGCGGGCTTGGTCATCGTATGCGCGATTGTCCAAAATTAGAACGTCATAATAGTGTGGCAAATGCAAATAATAGGAAGGATTGTTTTGGATCTGGAGGTTACAAAGGAGAAATTTGA
Protein sequence

>MS.gene32424.t1

MVAKMEEDDYEEYIPVAKRRAMEAQKILQRKGKLSAPIDDDSRKLSVVETKPSLLVKASQLKKEQPEISVTEQIVQQEKEMIENLSDKKTLMSVRELAKGITYNEPLPTGWKPPLHIRRMTKKDCDLIQKQWHIIVNGEDIPPAIKNFKDMRFPDPILKMLKTKGIVQPTPIQVQGLPVILSGRDMIGIAFTGSGKTLVFVLPMIMMAMQEEITMPVVPGEGPFGLIICPSRELARQTYEVIEQFLLPLKESGYPELRPLLCIGGIDTRSQLEIVKKGVHIVVATPGRLKDMLAKKKMNLDNCRYLTLDEADRLVDPGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFAKSALVKPIIVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIHEYLLLKGVEAVAIHGDKDQEDREYAISSFKTGKKDVLVATDIASKGLDFPDIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQTETTLLDLKHLLQEAKQRIPPVLAELIDPMEDNEEITGISGVKGCAFCCGLGHRMRDCPKLERHNSVANANNRKDCFGSGGYKGEI