Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33166.t1 | XP_003603321.1 | 100 | 51 | 0 | 0 | 1 | 51 | 1 | 51 | 6.60E-21 | 109 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33166.t1 | Q5SMI4 | 98.0 | 51 | 1 | 0 | 1 | 51 | 1 | 51 | 7.9e-23 | 106.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33166.t1 | G7J9X2 | 100.0 | 51 | 0 | 0 | 1 | 51 | 1 | 51 | 4.7e-21 | 109.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051904 | MS.gene33166 | 0.846044 | 2.78E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene33166 | MS.gene016022 | PPI |
| MS.gene33166 | MS.gene060917 | PPI |
| MS.gene61173 | MS.gene33166 | PPI |
| MS.gene33166 | MS.gene61173 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33166.t1 | MTR_3g106310 | 100.000 | 51 | 0 | 0 | 1 | 51 | 1 | 51 | 3.35e-32 | 105 |
| MS.gene33166.t1 | MTR_1g013070 | 100.000 | 51 | 0 | 0 | 1 | 51 | 1 | 51 | 3.35e-32 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33166.t1 | AT4G31985 | 90.196 | 51 | 5 | 0 | 1 | 51 | 1 | 51 | 1.80e-29 | 98.2 |
| MS.gene33166.t1 | AT3G02190 | 88.235 | 51 | 6 | 0 | 1 | 51 | 1 | 51 | 4.22e-28 | 94.7 |
| MS.gene33166.t1 | AT2G25210 | 90.909 | 44 | 4 | 0 | 8 | 51 | 1 | 44 | 6.32e-24 | 84.0 |
Find 14 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTCTCATACGGATCCAATA+AGG | 0.402869 | 1.3:+8756022 | None:intergenic |
| AGAACAGACCCATCCCTTAT+TGG | 0.418161 | 1.3:-8756036 | MS.gene33166:CDS |
| TTCTCATACGGATCCAATAA+GGG | 0.468482 | 1.3:+8756023 | None:intergenic |
| AATTAACCTGATGGTGTTGT+CGG | 0.485237 | 1.3:+8756000 | None:intergenic |
| CACTGGCGTCGTACCAAGCT+CGG | 0.498772 | 1.3:-8755867 | MS.gene33166:CDS |
| TGAGAACCGACAACACCATC+AGG | 0.505441 | 1.3:-8756006 | MS.gene33166:intron |
| AACACCTCAGAATCCGAGCT+TGG | 0.538065 | 1.3:+8755854 | None:intergenic |
| TGGTGTTGTCGGTTCTCATA+CGG | 0.540409 | 1.3:+8756011 | None:intergenic |
| ACAATGCTAAGCGTCGTCAC+TGG | 0.548403 | 1.3:-8755884 | MS.gene33166:CDS |
| CGTACCAAGCTCGGATTCTG+AGG | 0.575484 | 1.3:-8755858 | None:intergenic |
| AGAAGCTCGCGAAGAAGATG+AGG | 0.578712 | 1.3:-8756060 | MS.gene33166:CDS |
| CATACGGATCCAATAAGGGA+TGG | 0.584103 | 1.3:+8756027 | None:intergenic |
| ATGACGAATGTCTTGTGTGA+AGG | 0.588940 | 1.3:+8756088 | None:intergenic |
| ATACGGATCCAATAAGGGAT+GGG | 0.595390 | 1.3:+8756028 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTGTTATTTCATTTGATTTT+AGG | - | chr1.3:8756145-8756164 | MS.gene33166:intron | 15.0% |
| ! | ATAGAAAGAAATTAACCTGA+TGG | + | chr1.3:8756065-8756084 | None:intergenic | 25.0% |
| ! | TAATCGTGTTAAATCATGTT+TGG | - | chr1.3:8756110-8756129 | MS.gene33166:intron | 25.0% |
| AGAAATTGAATCGAGAATAC+TGG | + | chr1.3:8755912-8755931 | None:intergenic | 30.0% | |
| AGAATACTGGAATTAGGAAT+AGG | + | chr1.3:8755899-8755918 | None:intergenic | 30.0% | |
| GAATCGAGAATACTGGAATT+AGG | + | chr1.3:8755905-8755924 | None:intergenic | 35.0% | |
| TTAACAGTACAACGTTGTTG+TGG | + | chr1.3:8756093-8756112 | None:intergenic | 35.0% | |
| TTCTCATACGGATCCAATAA+GGG | + | chr1.3:8756033-8756052 | None:intergenic | 35.0% | |
| !! | AATTAACCTGATGGTGTTGT+CGG | + | chr1.3:8756056-8756075 | None:intergenic | 35.0% |
| ATACGGATCCAATAAGGGAT+GGG | + | chr1.3:8756028-8756047 | None:intergenic | 40.0% | |
| ATGACGAATGTCTTGTGTGA+AGG | + | chr1.3:8755968-8755987 | None:intergenic | 40.0% | |
| GTTCTCATACGGATCCAATA+AGG | + | chr1.3:8756034-8756053 | None:intergenic | 40.0% | |
| AGAACAGACCCATCCCTTAT+TGG | - | chr1.3:8756017-8756036 | MS.gene33166:CDS | 45.0% | |
| CATACGGATCCAATAAGGGA+TGG | + | chr1.3:8756029-8756048 | None:intergenic | 45.0% | |
| !! | TGGTGTTGTCGGTTCTCATA+CGG | + | chr1.3:8756045-8756064 | None:intergenic | 45.0% |
| AGAAGCTCGCGAAGAAGATG+AGG | - | chr1.3:8755993-8756012 | MS.gene33166:intron | 50.0% | |
| TGAGAACCGACAACACCATC+AGG | - | chr1.3:8756047-8756066 | MS.gene33166:CDS | 50.0% | |
| ! | ACAATGCTAAGCGTCGTCAC+TGG | - | chr1.3:8756169-8756188 | MS.gene33166:intron | 50.0% |
| CACTGGCGTCGTACCAAGCT+CGG | - | chr1.3:8756186-8756205 | MS.gene33166:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 8755860 | 8756215 | 8755860 | ID=MS.gene33166 |
| chr1.3 | mRNA | 8755860 | 8756215 | 8755860 | ID=MS.gene33166.t1;Parent=MS.gene33166 |
| chr1.3 | exon | 8756213 | 8756215 | 8756213 | ID=MS.gene33166.t1.exon1;Parent=MS.gene33166.t1 |
| chr1.3 | CDS | 8756213 | 8756215 | 8756213 | ID=cds.MS.gene33166.t1;Parent=MS.gene33166.t1 |
| chr1.3 | exon | 8756007 | 8756110 | 8756007 | ID=MS.gene33166.t1.exon2;Parent=MS.gene33166.t1 |
| chr1.3 | CDS | 8756007 | 8756110 | 8756007 | ID=cds.MS.gene33166.t1;Parent=MS.gene33166.t1 |
| chr1.3 | exon | 8755860 | 8755908 | 8755860 | ID=MS.gene33166.t1.exon3;Parent=MS.gene33166.t1 |
| chr1.3 | CDS | 8755860 | 8755908 | 8755860 | ID=cds.MS.gene33166.t1;Parent=MS.gene33166.t1 |
| Gene Sequence |
| Protein sequence |
