AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene051904


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051904.t1 MTR_0699s0020 100.000 112 0 0 1 112 1 112 2.57e-79 228
MS.gene051904.t1 MTR_0699s0010 99.107 112 1 0 1 112 1 112 1.25e-78 226
MS.gene051904.t1 MTR_5g081705 94.643 112 6 0 1 112 26 137 5.74e-77 223
MS.gene051904.t1 MTR_5g081705 94.949 99 5 0 1 99 26 124 2.98e-67 198
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051904.t1 AT1G74270 91.964 112 9 0 1 112 1 112 6.63e-74 214
MS.gene051904.t1 AT1G07070 91.071 112 10 0 1 112 1 112 7.40e-74 214
MS.gene051904.t1 AT3G55750 87.387 111 14 0 2 112 1 111 4.97e-71 207
MS.gene051904.t1 AT1G41880 86.486 111 15 0 2 112 1 111 7.54e-71 207
MS.gene051904.t1 AT1G41880 86.486 111 15 0 2 112 1 111 7.54e-71 207

Find 48 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGAAGATTTGACTTGAATT+TGG 0.232635 7.1:+6134268 None:intergenic
TTAAATTATTACCATTGATT+TGG 0.240677 7.1:+6134244 None:intergenic
TGGTGTTCACTCCTTCAATT+TGG 0.242520 7.1:+6134426 None:intergenic
TAAATTATTACCATTGATTT+GGG 0.268294 7.1:+6134245 None:intergenic
GCCCCATATGCAGCGGTAAT+GGG 0.380708 7.1:+6134331 None:intergenic
CCAGAGATGTGTTTGGGTAC+TGG 0.398125 7.1:+6134450 None:intergenic
ATTTGGACCAGAGATGTGTT+TGG 0.405595 7.1:+6134443 None:intergenic
GAACCCATTACCGCTGCATA+TGG 0.409627 7.1:-6134334 MS.gene051904:CDS
TGCCCCATATGCAGCGGTAA+TGG 0.410420 7.1:+6134330 None:intergenic
GACGTCAAGGAGAGCGTGTC+AGG 0.412439 7.1:-6135105 MS.gene051904:intron
TTTGGACCAGAGATGTGTTT+GGG 0.422500 7.1:+6134444 None:intergenic
TTGGTATGCTGGCAAGAAAA+TGG 0.428506 7.1:-6134390 MS.gene051904:CDS
AGTTTACCTCATATATTGCT+TGG 0.429962 7.1:+6134116 None:intergenic
GAAGAGGTAGCTTGGTATGC+TGG 0.459649 7.1:-6134401 MS.gene051904:CDS
AACCCATTACCGCTGCATAT+GGG 0.459805 7.1:-6134333 MS.gene051904:CDS
ACATCTCTGGTCCAAATTGA+AGG 0.462436 7.1:-6134437 MS.gene051904:CDS
GTTTACCTCATATATTGCTT+GGG 0.467769 7.1:+6134117 None:intergenic
TACGTGAGGGGTACAATCCT+TGG 0.470557 7.1:-6134570 MS.gene051904:CDS
GGTTCCATTCTTCTTCACCT+TGG 0.492441 7.1:+6134352 None:intergenic
GTACAATCCTTGGATACAAA+AGG 0.498010 7.1:-6134560 MS.gene051904:intron
AAGGCCAAGGTGAAGAAGAA+TGG 0.517743 7.1:-6134356 MS.gene051904:CDS
GTTTGGGTACTGGTTTACTT+TGG 0.518411 7.1:+6134460 None:intergenic
ATACCAAGCTACCTCTTCCT+TGG 0.523366 7.1:+6134406 None:intergenic
GCATATGGGGCAAGGTTACA+AGG 0.526923 7.1:-6134319 MS.gene051904:CDS
CCAGTACCCAAACACATCTC+TGG 0.530530 7.1:-6134450 MS.gene051904:CDS
TTGTATTAGGCTTTACGTGA+GGG 0.533599 7.1:-6134583 MS.gene051904:intron
ACCCATTACCGCTGCATATG+GGG 0.539984 7.1:-6134332 MS.gene051904:CDS
TTTGTATTAGGCTTTACGTG+AGG 0.553810 7.1:-6134584 MS.gene051904:intron
AGGAGTGAACACCAAGGAAG+AGG 0.555068 7.1:-6134417 MS.gene051904:CDS
ATGTACCCAAGCAATATATG+AGG 0.556382 7.1:-6134122 None:intergenic
AATTATTACCATTGATTTGG+GGG 0.558553 7.1:+6134247 None:intergenic
GGCATATATCTACAAGGCCA+AGG 0.574061 7.1:-6134369 MS.gene051904:CDS
AAATTATTACCATTGATTTG+GGG 0.588097 7.1:+6134246 None:intergenic
ACACCAAGGAAGAGGTAGCT+TGG 0.593143 7.1:-6134409 MS.gene051904:CDS
GTACATAAATACTCTTACGC+GGG 0.601920 7.1:+6134139 None:intergenic
TGTATTAGGCTTTACGTGAG+GGG 0.604275 7.1:-6134582 MS.gene051904:intron
GGCAAGGTTACAAGGCCACA+TGG 0.606069 7.1:-6134311 MS.gene051904:CDS
AAGATGGTGAAGGGACGTCA+AGG 0.609822 7.1:-6135118 MS.gene051904:CDS
ATTATTACCATTGATTTGGG+GGG 0.627666 7.1:+6134248 None:intergenic
GAAAATGGCATATATCTACA+AGG 0.630318 7.1:-6134375 MS.gene051904:CDS
GGTACATAAATACTCTTACG+CGG 0.638869 7.1:+6134138 None:intergenic
TTACCGCTGCATATGGGGCA+AGG 0.645005 7.1:-6134327 MS.gene051904:CDS
AGTTGCCGTGAAGATGGTGA+AGG 0.661868 7.1:-6135128 None:intergenic
TAACCTTGCCCCATATGCAG+CGG 0.677626 7.1:+6134324 None:intergenic
ACAAGGCCACATGGTAACAG+TGG 0.690947 7.1:-6134302 MS.gene051904:CDS
GTTGCCGTGAAGATGGTGAA+GGG 0.718264 7.1:-6135127 None:intergenic
ACAATACCACTGTTACCATG+TGG 0.724821 7.1:+6134296 None:intergenic
AATTGAAGGAGTGAACACCA+AGG 0.776962 7.1:-6134423 MS.gene051904:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAATATTAGCTTATTATA+TGG + chr7.1:6134624-6134643 None:intergenic 10.0%
!!! TTTTTTTTTAATCATCATTT+GGG - chr7.1:6135031-6135050 MS.gene051904:intron 10.0%
!!! TTTTTTTTTTAATCATCATT+TGG - chr7.1:6135030-6135049 MS.gene051904:intron 10.0%
!!! AAACATGAAAATTTTCAAAT+TGG + chr7.1:6134533-6134552 None:intergenic 15.0%
!!! ATCATTGTTTTTAATTCATT+TGG - chr7.1:6134284-6134303 MS.gene051904:CDS 15.0%
!!! ATTTATTTTTGAATGATAGT+AGG + chr7.1:6134343-6134362 None:intergenic 15.0%
!!! ATTTTTGTTTCATTTGTATT+AGG - chr7.1:6134643-6134662 MS.gene051904:intron 15.0%
!!! TAAATTATTACCATTGATTT+GGG + chr7.1:6134997-6135016 None:intergenic 15.0%
!!! TTAAATTATTACCATTGATT+TGG + chr7.1:6134998-6135017 None:intergenic 15.0%
!!! AAATTATTACCATTGATTTG+GGG + chr7.1:6134996-6135015 None:intergenic 20.0%
! TAGTTTACAGAGTAAAGTAA+AGG - chr7.1:6134406-6134425 MS.gene051904:CDS 25.0%
!! ACTTTTCTAATGAAAATGTC+TGG - chr7.1:6134705-6134724 MS.gene051904:intron 25.0%
!! TATCAGACACTTATATTTTG+TGG - chr7.1:6134505-6134524 MS.gene051904:intron 25.0%
!!! AATTATTACCATTGATTTGG+GGG + chr7.1:6134995-6135014 None:intergenic 25.0%
ATCTGAAAATCAACTGTATC+TGG + chr7.1:6134481-6134500 None:intergenic 30.0%
GAAAATGGCATATATCTACA+AGG - chr7.1:6134864-6134883 MS.gene051904:intron 30.0%
TCGTGTTTGGATTAATTTCA+GGG - chr7.1:6135075-6135094 MS.gene051904:intron 30.0%
TGTTATCTTAATCTCGTGTT+TGG - chr7.1:6135062-6135081 MS.gene051904:intron 30.0%
! AAGTTTACCTTTTGTATCCA+AGG + chr7.1:6134689-6134708 None:intergenic 30.0%
!! ATTATTACCATTGATTTGGG+GGG + chr7.1:6134994-6135013 None:intergenic 30.0%
AAATGTCTGGTTAGATGCTT+TGG - chr7.1:6134718-6134737 MS.gene051904:intron 35.0%
CTCGTGTTTGGATTAATTTC+AGG - chr7.1:6135074-6135093 MS.gene051904:intron 35.0%
GAAAATCAACTGTATCTGGT+TGG + chr7.1:6134477-6134496 None:intergenic 35.0%
GGTACATAAATACTCTTACG+CGG + chr7.1:6135104-6135123 None:intergenic 35.0%
GTACAATCCTTGGATACAAA+AGG - chr7.1:6134679-6134698 MS.gene051904:intron 35.0%
GTACATAAATACTCTTACGC+GGG + chr7.1:6135103-6135122 None:intergenic 35.0%
TTGTATTAGGCTTTACGTGA+GGG - chr7.1:6134656-6134675 MS.gene051904:intron 35.0%
TTTGTATTAGGCTTTACGTG+AGG - chr7.1:6134655-6134674 MS.gene051904:intron 35.0%
!! GGGAAGATTTGACTTGAATT+TGG + chr7.1:6134974-6134993 None:intergenic 35.0%
AAATCTTCCCCCCAAATCAA+TGG - chr7.1:6134984-6135003 MS.gene051904:intron 40.0%
AATTGAAGGAGTGAACACCA+AGG - chr7.1:6134816-6134835 MS.gene051904:intron 40.0%
ACAATACCACTGTTACCATG+TGG + chr7.1:6134946-6134965 None:intergenic 40.0%
ACATCTCTGGTCCAAATTGA+AGG - chr7.1:6134802-6134821 MS.gene051904:intron 40.0%
ATTTGGACCAGAGATGTGTT+TGG + chr7.1:6134799-6134818 None:intergenic 40.0%
TGGTGTTCACTCCTTCAATT+TGG + chr7.1:6134816-6134835 None:intergenic 40.0%
TGTATTAGGCTTTACGTGAG+GGG - chr7.1:6134657-6134676 MS.gene051904:intron 40.0%
TTGGTATGCTGGCAAGAAAA+TGG - chr7.1:6134849-6134868 MS.gene051904:intron 40.0%
TTTGGACCAGAGATGTGTTT+GGG + chr7.1:6134798-6134817 None:intergenic 40.0%
!! GTTTGGGTACTGGTTTACTT+TGG + chr7.1:6134782-6134801 None:intergenic 40.0%
AACCCATTACCGCTGCATAT+GGG - chr7.1:6134906-6134925 MS.gene051904:intron 45.0%
AAGGCCAAGGTGAAGAAGAA+TGG - chr7.1:6134883-6134902 MS.gene051904:intron 45.0%
ATACCAAGCTACCTCTTCCT+TGG + chr7.1:6134836-6134855 None:intergenic 45.0%
GGCATATATCTACAAGGCCA+AGG - chr7.1:6134870-6134889 MS.gene051904:intron 45.0%
GGTTCCATTCTTCTTCACCT+TGG + chr7.1:6134890-6134909 None:intergenic 45.0%
ACAAGGCCACATGGTAACAG+TGG - chr7.1:6134937-6134956 MS.gene051904:intron 50.0%
ACACCAAGGAAGAGGTAGCT+TGG - chr7.1:6134830-6134849 MS.gene051904:intron 50.0%
ACCCATTACCGCTGCATATG+GGG - chr7.1:6134907-6134926 MS.gene051904:intron 50.0%
AGGAGTGAACACCAAGGAAG+AGG - chr7.1:6134822-6134841 MS.gene051904:intron 50.0%
CCAGAGATGTGTTTGGGTAC+TGG + chr7.1:6134792-6134811 None:intergenic 50.0%
CCAGTACCCAAACACATCTC+TGG - chr7.1:6134789-6134808 MS.gene051904:intron 50.0%
GAACCCATTACCGCTGCATA+TGG - chr7.1:6134905-6134924 MS.gene051904:intron 50.0%
GAAGAGGTAGCTTGGTATGC+TGG - chr7.1:6134838-6134857 MS.gene051904:intron 50.0%
GCATATGGGGCAAGGTTACA+AGG - chr7.1:6134920-6134939 MS.gene051904:intron 50.0%
TAACCTTGCCCCATATGCAG+CGG + chr7.1:6134918-6134937 None:intergenic 50.0%
! TACGTGAGGGGTACAATCCT+TGG - chr7.1:6134669-6134688 MS.gene051904:intron 50.0%
GCCCCATATGCAGCGGTAAT+GGG + chr7.1:6134911-6134930 None:intergenic 55.0%
GGCAAGGTTACAAGGCCACA+TGG - chr7.1:6134928-6134947 MS.gene051904:intron 55.0%
TGCCCCATATGCAGCGGTAA+TGG + chr7.1:6134912-6134931 None:intergenic 55.0%
TTACCGCTGCATATGGGGCA+AGG - chr7.1:6134912-6134931 MS.gene051904:intron 55.0%
! GACGTCAAGGAGAGCGTGTC+AGG - chr7.1:6134134-6134153 MS.gene051904:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 6134124 6135137 6134124 ID=MS.gene051904
chr7.1 mRNA 6134124 6135137 6134124 ID=MS.gene051904.t1;Parent=MS.gene051904
chr7.1 exon 6135106 6135137 6135106 ID=MS.gene051904.t1.exon1;Parent=MS.gene051904.t1
chr7.1 CDS 6135106 6135137 6135106 ID=cds.MS.gene051904.t1;Parent=MS.gene051904.t1
chr7.1 exon 6134561 6134596 6134561 ID=MS.gene051904.t1.exon2;Parent=MS.gene051904.t1
chr7.1 CDS 6134561 6134596 6134561 ID=cds.MS.gene051904.t1;Parent=MS.gene051904.t1
chr7.1 exon 6134256 6134484 6134256 ID=MS.gene051904.t1.exon3;Parent=MS.gene051904.t1
chr7.1 CDS 6134256 6134484 6134256 ID=cds.MS.gene051904.t1;Parent=MS.gene051904.t1
chr7.1 exon 6134124 6134165 6134124 ID=MS.gene051904.t1.exon4;Parent=MS.gene051904.t1
chr7.1 CDS 6134124 6134165 6134124 ID=cds.MS.gene051904.t1;Parent=MS.gene051904.t1
Gene Sequence

>MS.gene051904

ATGGTGAAGGGACGTCAAGGAGAGCGTGTCAGGTACTCTTCTTTTCATCTGATACTTGTAGATTTTGATCTGATTCTGTAGCTTGATCTCTTTCTTTTCTGCTATTGACTGTTTTTATTTTCATCATTATCTTATATGTGTTTGAATTTTAGTAGTAATTATCATTGTTTTTAATTCATTTGGCTATAGTTAGTTACTGTTAGTAATTTGATGATGCCTACTATCATTCAAAAATAAATATCTGATTTTGAGAAATTAGCATACTTTGAATAATTTCATTTTTAGTTTACAGAGTAAAGTAAAGGCATAATTGAATGTTGAAAAAAGCATGTATATCAATTTTCAAAGTACCAACCAGATACAGTTGATTTTCAGATAATGTATCAGACACTTATATTTTGTGGTACCAATTTGAAAATTTTCATGTTTTTCTCACTTAAAATCAGTTTTGTGTATTTACTAGACTAACTATGCGTATTTACTTTACTCTGTTTTTGCCATATAATAAGCTAATATTATATTTTTGTTTCATTTGTATTAGGCTTTACGTGAGGGGTACAATCCTTGGATACAAAAGGTAAACTTTTCTAATGAAAATGTCTGGTTAGATGCTTTGGCTTAATTAATACTTACTGTTTTATGCTGTTTTTTAGATCCAAAGTAAACCAGTACCCAAACACATCTCTGGTCCAAATTGAAGGAGTGAACACCAAGGAAGAGGTAGCTTGGTATGCTGGCAAGAAAATGGCATATATCTACAAGGCCAAGGTGAAGAAGAATGGAACCCATTACCGCTGCATATGGGGCAAGGTTACAAGGCCACATGGTAACAGTGGTATTGTTCGTGCCAAATTCAAGTCAAATCTTCCCCCCAAATCAATGGTAATAATTTAACAGTGTTTTTTTTTTTTTTTTTAATCATCATTTGGGTTTTATAATGTTATCTTAATCTCGTGTTTGGATTAATTTCAGGGTTCCCGCGTAAGAGTATTTATGTACCCAAGCAATATATGA

Protein sequence

>MS.gene051904.t1

MVKGRQGERVRLYVRGTILGYKRSKVNQYPNTSLVQIEGVNTKEEVAWYAGKKMAYIYKAKVKKNGTHYRCIWGKVTRPHGNSGIVRAKFKSNLPPKSMGSRVRVFMYPSNI