AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33519

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33519.t1 MTR_3g090120 98.575 351 5 0 1 351 115 465 0.0 727
MS.gene33519.t1 MTR_8g093530 60.417 336 129 1 15 350 90 421 2.78e-153 441
MS.gene33519.t1 MTR_7g094750 45.584 351 172 5 13 348 89 435 1.23e-107 325
MS.gene33519.t1 MTR_2g022560 41.972 355 188 5 13 353 95 445 1.12e-98 302
MS.gene33519.t1 MTR_7g109550 42.941 340 182 5 15 348 137 470 7.93e-98 300
MS.gene33519.t1 MTR_4g121560 42.029 345 185 4 14 346 113 454 4.22e-93 288
MS.gene33519.t1 MTR_1g058380 39.773 352 199 6 13 353 53 402 2.46e-91 282
MS.gene33519.t1 MTR_7g094750 51.852 243 113 1 13 255 89 327 7.93e-90 275
MS.gene33519.t1 MTR_5g059400 38.000 350 197 5 12 346 172 516 6.17e-87 274
MS.gene33519.t1 MTR_1g116130 38.000 350 197 5 12 346 172 516 6.17e-87 274
MS.gene33519.t1 MTR_5g033800 38.440 359 199 6 4 347 134 485 4.49e-85 268
MS.gene33519.t1 MTR_7g093450 39.227 362 198 6 2 349 238 591 1.12e-84 270
MS.gene33519.t1 MTR_0136s0010 40.230 348 189 5 15 348 170 512 2.89e-84 267
MS.gene33519.t1 MTR_8g069790 39.889 361 193 9 3 347 166 518 3.23e-83 264
MS.gene33519.t1 MTR_4g126950 36.932 352 204 7 15 353 109 455 6.52e-83 261
MS.gene33519.t1 MTR_2g073500 38.372 344 182 9 12 347 26 347 5.72e-76 240
MS.gene33519.t1 MTR_5g034810 37.822 349 188 10 7 347 27 354 1.11e-72 232
MS.gene33519.t1 MTR_3g464570 36.011 361 188 9 9 346 37 377 1.12e-71 230
MS.gene33519.t1 MTR_2g015720 38.261 345 187 9 7 345 34 358 1.62e-70 226
MS.gene33519.t1 MTR_1g111010 37.830 341 183 12 10 346 27 342 2.49e-70 226
MS.gene33519.t1 MTR_5g071350 34.232 371 197 9 5 346 48 400 7.14e-70 226
MS.gene33519.t1 MTR_4g088030 38.166 338 184 8 15 346 33 351 6.22e-69 222
MS.gene33519.t1 MTR_3g035830 34.203 345 186 8 13 343 29 346 7.35e-69 222
MS.gene33519.t1 MTR_3g021060 35.207 338 192 8 13 343 44 361 8.43e-69 222
MS.gene33519.t1 MTR_4g016940 34.270 356 199 8 13 345 84 427 1.05e-68 224
MS.gene33519.t1 MTR_3g049330 35.227 352 203 7 2 346 18 351 1.09e-68 221
MS.gene33519.t1 MTR_4g079700 37.609 343 189 8 8 345 41 363 1.14e-68 222
MS.gene33519.t1 MTR_4g087730 37.574 338 186 7 15 346 45 363 2.12e-68 221
MS.gene33519.t1 MTR_2g073510 38.810 353 186 10 3 345 21 353 7.91e-68 219
MS.gene33519.t1 MTR_3g021090 34.985 343 182 8 13 341 29 344 1.97e-67 220
MS.gene33519.t1 MTR_5g033800 40.873 252 143 2 4 255 134 379 6.48e-67 218
MS.gene33519.t1 MTR_4g014440 31.622 370 220 8 3 348 50 410 2.64e-64 212
MS.gene33519.t1 MTR_1g050448 34.783 345 197 7 11 345 49 375 2.32e-62 206
MS.gene33519.t1 MTR_2g091175 36.202 337 191 7 15 346 50 367 6.26e-62 204
MS.gene33519.t1 MTR_8g463790 32.249 369 210 9 3 347 59 411 7.01e-62 206
MS.gene33519.t1 MTR_1g112210 35.838 346 185 8 15 345 59 382 7.99e-62 204
MS.gene33519.t1 MTR_7g068920 33.795 361 194 9 13 345 78 421 1.80e-60 202
MS.gene33519.t1 MTR_1g112210 35.465 344 176 7 15 345 59 369 7.77e-60 199
MS.gene33519.t1 MTR_3g070900 31.215 362 212 8 10 346 101 450 4.32e-59 199
MS.gene33519.t1 MTR_7g070060 30.749 374 215 10 7 348 51 412 6.70e-59 197
MS.gene33519.t1 MTR_4g014430 31.476 359 208 9 10 345 103 446 2.69e-58 197
MS.gene33519.t1 MTR_5g082880 30.000 360 216 8 15 348 165 514 3.80e-56 193
MS.gene33519.t1 MTR_3g071650 30.878 353 213 8 15 346 89 431 1.38e-54 187
MS.gene33519.t1 MTR_5g083260 30.447 358 216 8 11 346 79 425 2.95e-54 186
MS.gene33519.t1 MTR_3g464570 35.889 287 145 9 83 346 3 273 1.60e-47 164
MS.gene33519.t1 MTR_6g061710 29.134 381 205 12 14 353 166 522 6.27e-43 157
MS.gene33519.t1 MTR_7g070060 33.852 257 149 6 7 254 51 295 1.85e-42 152
MS.gene33519.t1 MTR_4g125060 30.726 358 205 15 14 345 131 471 1.04e-38 145
MS.gene33519.t1 MTR_4g125060 30.141 355 211 13 14 345 170 510 1.49e-38 145
MS.gene33519.t1 MTR_4g125060 30.726 358 205 15 14 345 199 539 2.21e-38 145
MS.gene33519.t1 MTR_8g104970 28.800 375 210 14 7 345 112 465 8.87e-38 143
MS.gene33519.t1 MTR_1g076690 29.301 372 212 14 14 351 222 576 1.69e-37 143
MS.gene33519.t1 MTR_5g035150 29.730 370 200 13 14 345 84 431 2.23e-37 141
MS.gene33519.t1 MTR_5g035090 28.800 375 219 12 7 350 75 432 2.40e-37 141
MS.gene33519.t1 MTR_5g035080 29.443 377 213 15 7 349 67 424 3.70e-37 140
MS.gene33519.t1 MTR_7g033230 28.533 375 197 13 14 345 263 609 1.18e-36 141
MS.gene33519.t1 MTR_7g033230 28.533 375 197 13 14 345 333 679 2.00e-36 141
MS.gene33519.t1 MTR_7g070060 27.541 305 185 8 71 348 15 310 2.18e-36 135
MS.gene33519.t1 MTR_8g093870 27.451 357 238 10 4 345 122 472 2.26e-36 139
MS.gene33519.t1 MTR_3g109840 29.787 376 210 15 3 345 61 415 5.06e-36 137
MS.gene33519.t1 MTR_4g092830 27.596 366 211 14 15 346 48 393 5.81e-36 137
MS.gene33519.t1 MTR_2g022580 47.967 123 64 0 79 201 2 124 1.87e-35 127
MS.gene33519.t1 MTR_6g007777 28.418 373 230 13 7 355 108 467 3.52e-32 127
MS.gene33519.t1 MTR_6g016880 26.923 364 223 11 9 345 72 419 7.11e-31 123
MS.gene33519.t1 MTR_8g027900 27.200 375 226 13 3 346 198 556 8.68e-31 124
MS.gene33519.t1 MTR_5g035070 29.670 364 208 14 14 345 86 433 3.91e-30 121
MS.gene33519.t1 MTR_6g016875 27.441 379 224 12 1 347 50 409 7.85e-29 117
MS.gene33519.t1 MTR_6g016240 26.087 368 231 14 6 345 62 416 5.56e-28 114
MS.gene33519.t1 MTR_7g019120 25.753 365 224 12 10 345 74 420 1.19e-27 114
MS.gene33519.t1 MTR_8g099300 25.401 374 212 15 15 347 74 421 1.26e-27 114
MS.gene33519.t1 MTR_2g016060 26.431 367 228 13 10 348 50 402 1.87e-26 110
MS.gene33519.t1 MTR_5g035120 43.617 94 49 1 14 107 74 163 5.09e-22 92.8
MS.gene33519.t1 MTR_7g019140 38.384 99 57 1 9 107 73 167 3.66e-19 84.7
MS.gene33519.t1 MTR_5g069320 26.644 289 148 11 65 338 147 386 2.27e-17 83.6
MS.gene33519.t1 MTR_5g035140 26.400 250 138 10 136 348 28 268 2.17e-13 70.5
MS.gene33519.t1 MTR_3g021150 30.952 126 60 4 222 333 6 118 1.72e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33519.t1 AT1G48880 62.139 346 125 2 3 348 103 442 3.12e-163 466
MS.gene33519.t1 AT3G06080 41.176 357 186 5 11 349 99 449 9.53e-96 295
MS.gene33519.t1 AT3G62390 40.960 354 194 5 4 348 127 474 2.07e-95 294
MS.gene33519.t1 AT5G19160 40.443 361 186 6 11 349 95 448 3.58e-94 291
MS.gene33519.t1 AT5G06230 41.908 346 186 7 15 346 21 365 5.16e-89 275
MS.gene33519.t1 AT5G49340 41.143 350 183 8 14 346 99 442 1.75e-88 276
MS.gene33519.t1 AT5G06230 41.360 353 192 7 8 346 55 406 1.87e-88 275
MS.gene33519.t1 AT3G11570 42.470 332 172 6 35 353 98 423 3.41e-88 274
MS.gene33519.t1 AT1G60790 38.764 356 200 6 7 347 182 534 9.58e-84 266
MS.gene33519.t1 AT5G20590 36.812 345 202 6 14 346 140 480 1.27e-82 261
MS.gene33519.t1 AT3G12060 38.611 360 196 7 3 348 190 538 1.32e-82 264
MS.gene33519.t1 AT5G06700 39.943 348 190 6 15 348 255 597 2.41e-82 265
MS.gene33519.t1 AT2G34070 37.644 348 193 7 4 345 54 383 1.75e-78 248
MS.gene33519.t1 AT5G19160 44.400 250 135 1 11 260 95 340 4.10e-78 246
MS.gene33519.t1 AT3G06080 46.939 245 126 1 11 255 99 339 5.71e-78 245
MS.gene33519.t1 AT1G29050 38.462 338 183 7 14 345 60 378 1.97e-75 240
MS.gene33519.t1 AT3G14850 36.522 345 193 7 11 347 30 356 1.71e-72 231
MS.gene33519.t1 AT5G06230 43.200 250 140 1 8 255 55 304 2.33e-68 219
MS.gene33519.t1 AT1G78710 36.522 345 181 9 15 346 38 357 4.00e-68 220
MS.gene33519.t1 AT3G62390 40.221 271 156 3 4 274 127 391 4.48e-68 222
MS.gene33519.t1 AT2G42570 34.483 348 205 6 4 346 36 365 5.72e-67 218
MS.gene33519.t1 AT5G58600 33.799 358 197 11 11 346 62 401 2.22e-66 217
MS.gene33519.t1 AT2G30010 33.973 365 195 11 10 346 51 397 4.64e-64 211
MS.gene33519.t1 AT5G01360 32.123 358 205 10 13 345 34 378 7.20e-64 210
MS.gene33519.t1 AT2G30010 33.973 365 195 11 10 346 94 440 9.24e-64 211
MS.gene33519.t1 AT2G31110 34.985 343 200 7 8 345 38 362 1.23e-63 209
MS.gene33519.t1 AT2G30900 36.127 346 193 11 12 347 40 367 1.68e-63 208
MS.gene33519.t1 AT1G73140 33.983 359 197 9 13 347 65 407 2.13e-63 209
MS.gene33519.t1 AT5G01360 32.123 358 205 10 13 345 90 434 2.15e-63 210
MS.gene33519.t1 AT3G54260 36.599 347 183 10 15 346 51 375 6.19e-62 205
MS.gene33519.t1 AT5G20590 38.430 242 145 2 14 255 140 377 1.84e-61 203
MS.gene33519.t1 AT5G01620 32.102 352 206 8 15 345 110 449 1.18e-59 201
MS.gene33519.t1 AT5G01620 32.102 352 206 8 15 345 110 449 1.18e-59 201
MS.gene33519.t1 AT5G01620 32.102 352 206 8 15 345 118 457 1.26e-59 201
MS.gene33519.t1 AT2G38320 30.704 355 215 7 15 346 59 405 1.16e-58 197
MS.gene33519.t1 AT2G40160 29.973 367 219 9 10 349 72 427 1.74e-58 197
MS.gene33519.t1 AT2G40160 30.055 366 218 9 10 348 72 426 2.09e-58 197
MS.gene33519.t1 AT2G40150 29.891 368 213 10 13 348 69 423 1.49e-55 189
MS.gene33519.t1 AT3G55990 29.640 361 217 8 13 348 138 486 1.30e-53 186
MS.gene33519.t1 AT3G11030 30.790 367 200 9 13 346 104 449 2.12e-53 184
MS.gene33519.t1 AT2G40320 30.447 358 216 9 11 346 77 423 3.33e-53 183
MS.gene33519.t1 AT5G58600 36.179 246 137 6 11 254 62 289 2.19e-50 172
MS.gene33519.t1 AT2G38320 29.907 321 197 6 49 346 7 322 1.84e-49 171
MS.gene33519.t1 AT2G34070 36.122 263 148 6 89 345 1 249 5.40e-49 167
MS.gene33519.t1 AT1G78710 33.333 333 155 12 15 346 31 297 7.91e-48 166
MS.gene33519.t1 AT2G30900 39.331 239 125 7 12 248 40 260 1.48e-47 164
MS.gene33519.t1 AT3G14850 35.581 267 150 6 89 347 1 253 1.04e-45 159
MS.gene33519.t1 AT2G31110 37.154 253 137 6 8 260 38 268 1.88e-45 158
MS.gene33519.t1 AT1G70230 33.151 365 178 17 15 345 79 411 2.08e-42 154
MS.gene33519.t1 AT2G40160 33.068 251 150 6 10 254 72 310 2.29e-42 151
MS.gene33519.t1 AT5G64470 30.601 366 214 14 6 346 45 395 9.12e-41 149
MS.gene33519.t1 AT5G64470 29.839 372 215 14 6 346 45 401 1.11e-38 144
MS.gene33519.t1 AT4G25360 29.111 371 209 15 15 349 175 527 1.13e-38 146
MS.gene33519.t1 AT4G25360 29.111 371 209 15 15 349 175 527 1.13e-38 146
MS.gene33519.t1 AT5G51640 31.135 379 206 18 4 345 128 488 6.31e-37 141
MS.gene33519.t1 AT5G20680 27.824 363 221 14 8 345 111 457 1.31e-35 136
MS.gene33519.t1 AT4G01080 28.000 375 217 13 9 352 85 437 1.42e-35 136
MS.gene33519.t1 AT1G01430 25.197 381 235 10 3 349 87 451 1.58e-35 136
MS.gene33519.t1 AT5G20680 27.824 363 221 14 8 345 187 533 3.52e-35 136
MS.gene33519.t1 AT5G20680 27.824 363 221 14 8 345 205 551 4.17e-35 136
MS.gene33519.t1 AT5G20680 27.824 363 221 14 8 345 205 551 4.17e-35 136
MS.gene33519.t1 AT5G20680 27.824 363 221 14 8 345 205 551 4.17e-35 136
MS.gene33519.t1 AT5G20680 27.824 363 221 14 8 345 205 551 4.17e-35 136
MS.gene33519.t1 AT5G64020 27.701 361 224 12 14 345 56 408 7.63e-35 134
MS.gene33519.t1 AT5G15890 29.379 354 209 14 15 345 187 522 2.31e-34 134
MS.gene33519.t1 AT2G37720 27.147 361 226 12 8 345 136 482 8.02e-34 132
MS.gene33519.t1 AT4G11090 28.460 383 212 16 4 347 68 427 9.00e-34 131
MS.gene33519.t1 AT3G02440 29.189 370 203 13 15 349 127 472 1.29e-33 131
MS.gene33519.t1 AT3G02440 29.189 370 203 13 15 349 135 480 1.48e-33 131
MS.gene33519.t1 AT2G14530 29.006 362 209 14 15 345 62 406 9.86e-33 128
MS.gene33519.t1 AT2G31110 33.491 212 129 4 139 345 10 214 2.05e-30 117
MS.gene33519.t1 AT4G23790 28.912 377 218 15 4 346 68 428 5.31e-30 120
MS.gene33519.t1 AT5G15900 27.100 369 225 14 10 350 62 414 3.12e-29 118
MS.gene33519.t1 AT5G15900 27.100 369 225 14 10 350 81 433 4.20e-29 118
MS.gene33519.t1 AT3G28150 26.389 360 221 13 15 347 70 412 5.23e-26 109
MS.gene33519.t1 AT5G64470 30.576 278 166 11 6 269 45 309 4.52e-25 105
MS.gene33519.t1 AT5G64470 30.576 278 166 11 6 269 45 309 5.14e-25 104

Find 84 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAAGATCACCAAACAAATT+AGG 0.268500 3.3:+75722214 MS.gene33519:CDS
CTAGGAATATCACAGTTCTT+AGG 0.278013 3.3:-75722042 None:intergenic
GATGTTCTTATATTCAATTC+AGG 0.293943 3.3:+75722399 MS.gene33519:CDS
ACCAAACAAATTAGGTTTCT+AGG 0.294028 3.3:+75722222 MS.gene33519:CDS
CAAACCACCAGCATAAATTA+TGG 0.295629 3.3:+75724191 MS.gene33519:CDS
ATGCTTGCAAAGTGACTAAA+AGG 0.302638 3.3:+75723436 MS.gene33519:CDS
AAGATAGAGGTTATACAAAA+TGG 0.305284 3.3:+75722010 MS.gene33519:CDS
AGAATTTCATTCCACATATC+AGG 0.307439 3.3:-75723654 None:intergenic
TAGTCACTTTGCAAGCATTA+TGG 0.311847 3.3:-75723431 None:intergenic
ACCTAGAAACCTAATTTGTT+TGG 0.335765 3.3:-75722223 None:intergenic
CTTTGCTCACCTGGGCATCT+TGG 0.341521 3.3:+75723174 MS.gene33519:CDS
GTTTGATGGAAGTTGGATTC+AGG 0.354028 3.3:+75721897 MS.gene33519:CDS
CATTGAGAAGCATCATATAA+AGG 0.356973 3.3:-75721943 None:intergenic
TCAAAACAGCTTTGCTCACC+TGG 0.358303 3.3:+75723165 MS.gene33519:CDS
TTTGCTCACCTGGGCATCTT+GGG 0.367494 3.3:+75723175 MS.gene33519:CDS
ATGCTAAATACTTGCTTAAG+TGG 0.380270 3.3:+75724143 MS.gene33519:CDS
TTAAAACCTCTTTCTGCAAA+TGG 0.381291 3.3:-75721967 None:intergenic
CAATGTCCATTTGCAGAAAG+AGG 0.381573 3.3:+75721961 MS.gene33519:CDS
AGTCCAATGGAAGGTATAGA+AGG 0.398503 3.3:+75724299 MS.gene33519:CDS
GTAATGTGTTTGATGGAAGT+TGG 0.416256 3.3:+75721890 MS.gene33519:CDS
CTAAGAACTGTGATATTCCT+AGG 0.417542 3.3:+75722043 MS.gene33519:CDS
CATCTGAATCAACCCATTCA+TGG 0.430046 3.3:-75722380 None:intergenic
GGTGAAGACAACACTGAGAT+TGG 0.431850 3.3:+75722338 MS.gene33519:CDS
TGCAGTCATTGGTGTTTACC+TGG 0.434332 3.3:+75723627 MS.gene33519:CDS
TTATATTCAATTCAGGTCAC+TGG 0.436002 3.3:+75722406 MS.gene33519:CDS
TGCCTAGATTTGCGCCGAAA+AGG 0.436178 3.3:+75722316 MS.gene33519:CDS
CTGTACCTGATTGCAGTCAT+TGG 0.440386 3.3:+75723616 MS.gene33519:CDS
CTCTAAAATCCAATGTTTCA+AGG 0.441697 3.3:+75721860 MS.gene33519:CDS
AGGCCTTGGAAGAGAACTAT+AGG 0.446798 3.3:+75723456 MS.gene33519:CDS
GATGCATGTAATGTGTTTGA+TGG 0.447224 3.3:+75721883 MS.gene33519:CDS
ATTGAGAAGCATCATATAAA+GGG 0.448483 3.3:-75721942 None:intergenic
GCGAATGGGCGTAAAGATAG+AGG 0.453818 3.3:+75721997 MS.gene33519:CDS
TAGATGATATTAGCCATGAA+TGG 0.454788 3.3:+75722367 MS.gene33519:CDS
GCAAAGTGACTAAAAGGCCT+TGG 0.463270 3.3:+75723442 MS.gene33519:CDS
CTTTCGAATCATCGCATTGG+AGG 0.470520 3.3:+75723243 MS.gene33519:CDS
GCCTAGATTTGCGCCGAAAA+GGG 0.475483 3.3:+75722317 MS.gene33519:CDS
ACATGCATCCCTTGAAACAT+TGG 0.476011 3.3:-75721869 None:intergenic
ATTCATTGAGTAGGACTCAA+TGG 0.477060 3.3:+75722124 MS.gene33519:CDS
GTGATGGCCATGTGGGAACT+TGG 0.497057 3.3:+75723580 MS.gene33519:CDS
AGACGTGCACAGATAAGTAT+TGG 0.501206 3.3:+75724230 MS.gene33519:CDS
TTGCCTATAGTTCTCTTCCA+AGG 0.505458 3.3:-75723459 None:intergenic
TTAAGTGGTTGCATCACAAA+AGG 0.505867 3.3:+75724158 MS.gene33519:CDS
TGACGCCTATGGATGCATAT+AGG 0.507975 3.3:+75723556 MS.gene33519:CDS
AATGCGATGATTCGAAAGTT+CGG 0.509064 3.3:-75723238 None:intergenic
TCTAGATCCATAATTTATGC+TGG 0.509364 3.3:-75724198 None:intergenic
TACCTGGTGTTCCTGATATG+TGG 0.510262 3.3:+75723643 MS.gene33519:CDS
GACTAAAATTATATGATGCT+CGG 0.510666 3.3:-75724280 None:intergenic
GCAAATCTAGGCACCGAACC+AGG 0.516119 3.3:-75722306 None:intergenic
AGATGATATTAGCCATGAAT+GGG 0.516712 3.3:+75722368 MS.gene33519:CDS
TTGTTGGTGATTCATTGAGT+AGG 0.521972 3.3:+75722115 MS.gene33519:CDS
AAACACCAATGACTGCAATC+AGG 0.522147 3.3:-75723621 None:intergenic
GAACTTTCGAATCATCGCAT+TGG 0.522383 3.3:+75723240 MS.gene33519:CDS
TTCATTGAGTAGGACTCAAT+GGG 0.525301 3.3:+75722125 MS.gene33519:CDS
CAACCTTCTATACCTTCCAT+TGG 0.526860 3.3:-75724302 None:intergenic
GGTGCTGCACGTGACGCCTA+TGG 0.528371 3.3:+75723545 MS.gene33519:CDS
GTTGGTAGCTCACTGAAGCT+TGG 0.529154 3.3:+75723122 MS.gene33519:CDS
TCTAAAATCCAATGTTTCAA+GGG 0.532501 3.3:+75721861 MS.gene33519:CDS
GAGGTTATACAAAATGGAGA+TGG 0.534592 3.3:+75722016 MS.gene33519:CDS
CAAAACAGCTTTGCTCACCT+GGG 0.540720 3.3:+75723166 MS.gene33519:CDS
TATTCAATTCAGGTCACTGG+TGG 0.549775 3.3:+75722409 MS.gene33519:CDS
AGATCCATAATTTATGCTGG+TGG 0.550026 3.3:-75724195 None:intergenic
AAGAGTGTTTATGAAATCAA+AGG 0.567848 3.3:+75722189 MS.gene33519:CDS
GCATCACAAAAGGTTCAAGT+TGG 0.568990 3.3:+75724168 MS.gene33519:CDS
ATGGATGCATATAGGAGTGA+TGG 0.573342 3.3:+75723564 MS.gene33519:CDS
GTCACGTGCAGCACCGTCAC+AGG 0.574534 3.3:-75723537 None:intergenic
ATTCAGGTCACTGGTGGACA+AGG 0.579515 3.3:+75722415 MS.gene33519:CDS
ATTATCTACCCAAGATGCCC+AGG 0.580934 3.3:-75723183 None:intergenic
TTGATATGTATGTTAATGAC+AGG 0.583676 3.3:+75722153 MS.gene33519:CDS
TCACTCCTATATGCATCCAT+AGG 0.585942 3.3:-75723561 None:intergenic
AAATTAGGTTTCTAGGTGTG+AGG 0.589767 3.3:+75722229 MS.gene33519:CDS
ACAAGTTCAGAGCTATGTTG+AGG 0.591680 3.3:+75721837 None:intergenic
TGACTGCAATCAGGTACAGA+TGG 0.595507 3.3:-75723612 None:intergenic
AGAATACTCGAAAATCTTCG+TGG 0.596909 3.3:+75722078 MS.gene33519:CDS
ATGGAAGGTATAGAAGGTTG+TGG 0.603516 3.3:+75724305 MS.gene33519:CDS
GAATACTCGAAAATCTTCGT+GGG 0.608334 3.3:+75722079 MS.gene33519:CDS
AAATCTTCGTGGGAAAAGAG+TGG 0.610197 3.3:+75722089 MS.gene33519:CDS
ATTCTACGTGCATCAAACCT+AGG 0.613739 3.3:-75722060 None:intergenic
TTCCACATATCAGGAACACC+AGG 0.639295 3.3:-75723645 None:intergenic
TGTCACTCCAAGTTCCCACA+TGG 0.643897 3.3:-75723587 None:intergenic
TTGGAAGAGAACTATAGGCA+AGG 0.649599 3.3:+75723461 MS.gene33519:CDS
GAAGGTATAGAAGGTTGTGG+TGG 0.661013 3.3:+75724308 MS.gene33519:CDS
TATAGGAGTGATGGCCATGT+GGG 0.667902 3.3:+75723573 MS.gene33519:CDS
AGATATGAGTGCTCCTGTGA+CGG 0.680460 3.3:+75723524 MS.gene33519:CDS
ATATAGGAGTGATGGCCATG+TGG 0.734312 3.3:+75723572 MS.gene33519:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAACAAAATGAATAATA+TGG - chr3.3:75723283-75723302 None:intergenic 15.0%
!! TAGCTAGTAATAATAAATTA+AGG + chr3.3:75723907-75723926 MS.gene33519:intron 15.0%
!! TGTTTAATATATATGGAAAT+TGG - chr3.3:75723773-75723792 None:intergenic 15.0%
!! TTGAACAATTATTTACAATA+GGG - chr3.3:75724052-75724071 None:intergenic 15.0%
!!! ATGATAGATATACATTTTTA+TGG + chr3.3:75722511-75722530 MS.gene33519:intron 15.0%
!!! ATTTTATCTAAAGAATTACA+GGG - chr3.3:75722803-75722822 None:intergenic 15.0%
!!! TTTTGCTTGTTTAATATATA+TGG - chr3.3:75723780-75723799 None:intergenic 15.0%
!! GAAATTTATGAATTGTAAGA+AGG + chr3.3:75722681-75722700 MS.gene33519:intron 20.0%
!! GTTGAACAATTATTTACAAT+AGG - chr3.3:75724053-75724072 None:intergenic 20.0%
!! TAACATTTACATGAGTAATT+AGG + chr3.3:75723813-75723832 MS.gene33519:intron 20.0%
!!! ATCATATAATTTTAGTCCAA+TGG + chr3.3:75724286-75724305 MS.gene33519:CDS 20.0%
!!! ATCATGCATATGATAATTTT+GGG + chr3.3:75722773-75722792 MS.gene33519:intron 20.0%
!!! CATTTTATCTAAAGAATTAC+AGG - chr3.3:75722804-75722823 None:intergenic 20.0%
!!! GATTAATTTTGCTAATCTTT+TGG + chr3.3:75723337-75723356 MS.gene33519:intron 20.0%
!!! TTAATTTTCATATTTGGATC+CGG + chr3.3:75722606-75722625 MS.gene33519:intron 20.0%
!!! TTGGACTTAATTTTCATATT+TGG + chr3.3:75722600-75722619 MS.gene33519:intron 20.0%
!!! TTTTGTTTACATGCTTTTTT+TGG + chr3.3:75722581-75722600 MS.gene33519:intron 20.0%
! AAGATAGAGGTTATACAAAA+TGG + chr3.3:75722010-75722029 MS.gene33519:CDS 25.0%
! ATAAGATCACCAAACAAATT+AGG + chr3.3:75722214-75722233 MS.gene33519:CDS 25.0%
! ATTTATGAATTGTAAGAAGG+TGG + chr3.3:75722684-75722703 MS.gene33519:intron 25.0%
! CAAATAGTGTCATGAAAAAT+TGG + chr3.3:75722916-75722935 MS.gene33519:intron 25.0%
! GACTAAAATTATATGATGCT+CGG - chr3.3:75724283-75724302 None:intergenic 25.0%
! GATGTTCTTATATTCAATTC+AGG + chr3.3:75722399-75722418 MS.gene33519:CDS 25.0%
! TTGATATGTATGTTAATGAC+AGG + chr3.3:75722153-75722172 MS.gene33519:CDS 25.0%
!! CATCATGCATATGATAATTT+TGG + chr3.3:75722772-75722791 MS.gene33519:intron 25.0%
!! CTTTTGATCATATCTGATAT+TGG - chr3.3:75723495-75723514 None:intergenic 25.0%
!! TAATTTTCATATTTGGATCC+GGG + chr3.3:75722607-75722626 MS.gene33519:intron 25.0%
!! TCTAAAATCCAATGTTTCAA+GGG + chr3.3:75721861-75721880 MS.gene33519:CDS 25.0%
!! TTTTAATAGAATTTCCATGC+AGG + chr3.3:75723015-75723034 MS.gene33519:intron 25.0%
!!! AAGAGTGTTTATGAAATCAA+AGG + chr3.3:75722189-75722208 MS.gene33519:CDS 25.0%
!!! ATTGAGAAGCATCATATAAA+GGG - chr3.3:75721945-75721964 None:intergenic 25.0%
!!! GTTTTGAAAGCTGAATTAAT+CGG - chr3.3:75723152-75723171 None:intergenic 25.0%
!!! TATAATTTTAGTCCAATGGA+AGG + chr3.3:75724290-75724309 MS.gene33519:CDS 25.0%
AACGAAGAGTTTACCATAAA+AGG + chr3.3:75724016-75724035 MS.gene33519:intron 30.0%
AATATTAATCCTAACCTGCA+TGG - chr3.3:75723032-75723051 None:intergenic 30.0%
ACCAAACAAATTAGGTTTCT+AGG + chr3.3:75722222-75722241 MS.gene33519:CDS 30.0%
ACGAAGAGTTTACCATAAAA+GGG + chr3.3:75724017-75724036 MS.gene33519:intron 30.0%
AGAATTTCATTCCACATATC+AGG - chr3.3:75723657-75723676 None:intergenic 30.0%
AGATGATATTAGCCATGAAT+GGG + chr3.3:75722368-75722387 MS.gene33519:CDS 30.0%
AGCAGCAATCACTTATAAAT+TGG - chr3.3:75724103-75724122 None:intergenic 30.0%
ATGCTAAATACTTGCTTAAG+TGG + chr3.3:75724143-75724162 MS.gene33519:CDS 30.0%
ATTATTACTAGCTAAAGACG+AGG - chr3.3:75723901-75723920 None:intergenic 30.0%
GTATTTAGCATTAAGTTCAC+TGG - chr3.3:75724134-75724153 None:intergenic 30.0%
TAGATGATATTAGCCATGAA+TGG + chr3.3:75722367-75722386 MS.gene33519:CDS 30.0%
TCTAGATCCATAATTTATGC+TGG - chr3.3:75724201-75724220 None:intergenic 30.0%
TTAAAACCTCTTTCTGCAAA+TGG - chr3.3:75721970-75721989 None:intergenic 30.0%
TTATATTCAATTCAGGTCAC+TGG + chr3.3:75722406-75722425 MS.gene33519:CDS 30.0%
TTATTACTAGCTAAAGACGA+GGG - chr3.3:75723900-75723919 None:intergenic 30.0%
! ATGAAAAATTGGTCTCTTAG+TGG + chr3.3:75722927-75722946 MS.gene33519:intron 30.0%
!! ACCTAGAAACCTAATTTGTT+TGG - chr3.3:75722226-75722245 None:intergenic 30.0%
!! CATTGAGAAGCATCATATAA+AGG - chr3.3:75721946-75721965 None:intergenic 30.0%
!! CTCTAAAATCCAATGTTTCA+AGG + chr3.3:75721860-75721879 MS.gene33519:CDS 30.0%
!!! GTTTTAACTGTTTTGCGAAT+GGG + chr3.3:75721983-75722002 MS.gene33519:CDS 30.0%
AAGAGTTTACCATAAAAGGG+AGG + chr3.3:75724020-75724039 MS.gene33519:intron 35.0%
AATGCGATGATTCGAAAGTT+CGG - chr3.3:75723241-75723260 None:intergenic 35.0%
ACATGTGATGTGTTAGTTAG+TGG + chr3.3:75723731-75723750 MS.gene33519:intron 35.0%
ACTCTTCGTTACAACACATT+TGG - chr3.3:75724006-75724025 None:intergenic 35.0%
AGAATACTCGAAAATCTTCG+TGG + chr3.3:75722078-75722097 MS.gene33519:CDS 35.0%
AGATCCATAATTTATGCTGG+TGG - chr3.3:75724198-75724217 None:intergenic 35.0%
ATAGAATTTCCATGCAGGTT+AGG + chr3.3:75723020-75723039 MS.gene33519:intron 35.0%
ATGCTTGCAAAGTGACTAAA+AGG + chr3.3:75723436-75723455 MS.gene33519:CDS 35.0%
ATTCATTGAGTAGGACTCAA+TGG + chr3.3:75722124-75722143 MS.gene33519:CDS 35.0%
CAAACCACCAGCATAAATTA+TGG + chr3.3:75724191-75724210 MS.gene33519:CDS 35.0%
CTAACATGATTATCAGACAG+GGG + chr3.3:75723086-75723105 MS.gene33519:intron 35.0%
CTAAGAACTGTGATATTCCT+AGG + chr3.3:75722043-75722062 MS.gene33519:CDS 35.0%
CTAGGAATATCACAGTTCTT+AGG - chr3.3:75722045-75722064 None:intergenic 35.0%
GAATACTCGAAAATCTTCGT+GGG + chr3.3:75722079-75722098 MS.gene33519:CDS 35.0%
GAGGTTATACAAAATGGAGA+TGG + chr3.3:75722016-75722035 MS.gene33519:CDS 35.0%
GATGCATGTAATGTGTTTGA+TGG + chr3.3:75721883-75721902 MS.gene33519:CDS 35.0%
GCTAACATGATTATCAGACA+GGG + chr3.3:75723085-75723104 MS.gene33519:intron 35.0%
GTAATGTGTTTGATGGAAGT+TGG + chr3.3:75721890-75721909 MS.gene33519:CDS 35.0%
TGCTAACATGATTATCAGAC+AGG + chr3.3:75723084-75723103 MS.gene33519:intron 35.0%
TTAAGTGGTTGCATCACAAA+AGG + chr3.3:75724158-75724177 MS.gene33519:CDS 35.0%
TTCATTGAGTAGGACTCAAT+GGG + chr3.3:75722125-75722144 MS.gene33519:CDS 35.0%
! AAATTAGGTTTCTAGGTGTG+AGG + chr3.3:75722229-75722248 MS.gene33519:CDS 35.0%
! TAGTCACTTTGCAAGCATTA+TGG - chr3.3:75723434-75723453 None:intergenic 35.0%
! TGTTTATCTTTTTGTGCCAG+TGG + chr3.3:75723402-75723421 MS.gene33519:intron 35.0%
! TTTGTTGTCATTGTCTCATC+TGG - chr3.3:75723312-75723331 None:intergenic 35.0%
!! TTGTTGGTGATTCATTGAGT+AGG + chr3.3:75722115-75722134 MS.gene33519:CDS 35.0%
!!! GGTTTTAACTGTTTTGCGAA+TGG + chr3.3:75721982-75722001 MS.gene33519:CDS 35.0%
AAACACCAATGACTGCAATC+AGG - chr3.3:75723624-75723643 None:intergenic 40.0%
AAATCTTCGTGGGAAAAGAG+TGG + chr3.3:75722089-75722108 MS.gene33519:CDS 40.0%
ACAGTAAAGCATGCATTACC+CGG - chr3.3:75722628-75722647 None:intergenic 40.0%
ACATGCATCCCTTGAAACAT+TGG - chr3.3:75721872-75721891 None:intergenic 40.0%
AGACGTGCACAGATAAGTAT+TGG + chr3.3:75724230-75724249 MS.gene33519:CDS 40.0%
AGCATTATGGTTATGTCCAC+TGG - chr3.3:75723421-75723440 None:intergenic 40.0%
ATGGATGCATATAGGAGTGA+TGG + chr3.3:75723564-75723583 MS.gene33519:CDS 40.0%
ATTCTACGTGCATCAAACCT+AGG - chr3.3:75722063-75722082 None:intergenic 40.0%
CAACAAGTCACATTCTCTTC+AGG - chr3.3:75723848-75723867 None:intergenic 40.0%
CAACCTTCTATACCTTCCAT+TGG - chr3.3:75724305-75724324 None:intergenic 40.0%
CAATGTCCATTTGCAGAAAG+AGG + chr3.3:75721961-75721980 MS.gene33519:CDS 40.0%
CATCTGAATCAACCCATTCA+TGG - chr3.3:75722383-75722402 None:intergenic 40.0%
CATTTGGATACACTTTGAGC+TGG - chr3.3:75723990-75724009 None:intergenic 40.0%
GAACTTTCGAATCATCGCAT+TGG + chr3.3:75723240-75723259 MS.gene33519:CDS 40.0%
GCATCACAAAAGGTTCAAGT+TGG + chr3.3:75724168-75724187 MS.gene33519:CDS 40.0%
GTTTGATGGAAGTTGGATTC+AGG + chr3.3:75721897-75721916 MS.gene33519:CDS 40.0%
TATTCAATTCAGGTCACTGG+TGG + chr3.3:75722409-75722428 MS.gene33519:CDS 40.0%
TCACTCCTATATGCATCCAT+AGG - chr3.3:75723564-75723583 None:intergenic 40.0%
TTGCCTATAGTTCTCTTCCA+AGG - chr3.3:75723462-75723481 None:intergenic 40.0%
TTGGAAGAGAACTATAGGCA+AGG + chr3.3:75723461-75723480 MS.gene33519:CDS 40.0%
! AGTCCAATGGAAGGTATAGA+AGG + chr3.3:75724299-75724318 MS.gene33519:CDS 40.0%
! AGTGTTGTCTTCACCCTTTT+CGG - chr3.3:75722333-75722352 None:intergenic 40.0%
! ATGGAAGGTATAGAAGGTTG+TGG + chr3.3:75724305-75724324 MS.gene33519:CDS 40.0%
! GGGAAAAGAGTGGTTTTTGT+TGG + chr3.3:75722099-75722118 MS.gene33519:CDS 40.0%
AGATATGAGTGCTCCTGTGA+CGG + chr3.3:75723524-75723543 MS.gene33519:CDS 45.0%
AGGCCTTGGAAGAGAACTAT+AGG + chr3.3:75723456-75723475 MS.gene33519:CDS 45.0%
ATATAGGAGTGATGGCCATG+TGG + chr3.3:75723572-75723591 MS.gene33519:CDS 45.0%
ATTATCTACCCAAGATGCCC+AGG - chr3.3:75723186-75723205 None:intergenic 45.0%
CAAAACAGCTTTGCTCACCT+GGG + chr3.3:75723166-75723185 MS.gene33519:CDS 45.0%
CTGTACCTGATTGCAGTCAT+TGG + chr3.3:75723616-75723635 MS.gene33519:CDS 45.0%
CTTTCGAATCATCGCATTGG+AGG + chr3.3:75723243-75723262 MS.gene33519:CDS 45.0%
GCAAAGTGACTAAAAGGCCT+TGG + chr3.3:75723442-75723461 MS.gene33519:CDS 45.0%
GGTGAAGACAACACTGAGAT+TGG + chr3.3:75722338-75722357 MS.gene33519:CDS 45.0%
TACCTGGTGTTCCTGATATG+TGG + chr3.3:75723643-75723662 MS.gene33519:CDS 45.0%
TATAGGAGTGATGGCCATGT+GGG + chr3.3:75723573-75723592 MS.gene33519:CDS 45.0%
TCAAAACAGCTTTGCTCACC+TGG + chr3.3:75723165-75723184 MS.gene33519:CDS 45.0%
TGACGCCTATGGATGCATAT+AGG + chr3.3:75723556-75723575 MS.gene33519:CDS 45.0%
TGACTGCAATCAGGTACAGA+TGG - chr3.3:75723615-75723634 None:intergenic 45.0%
TTCCACATATCAGGAACACC+AGG - chr3.3:75723648-75723667 None:intergenic 45.0%
! GAAGGTATAGAAGGTTGTGG+TGG + chr3.3:75724308-75724327 MS.gene33519:CDS 45.0%
! TGCAGTCATTGGTGTTTACC+TGG + chr3.3:75723627-75723646 MS.gene33519:CDS 45.0%
! TTTTCTCGTGAAGCCTGGTT+CGG + chr3.3:75722293-75722312 MS.gene33519:CDS 45.0%
!! TCTGTTTTTCTCGTGAAGCC+TGG + chr3.3:75722288-75722307 MS.gene33519:CDS 45.0%
CATGATTATCAGACAGGGGC+TGG + chr3.3:75723090-75723109 MS.gene33519:intron 50.0%
GCCTAGATTTGCGCCGAAAA+GGG + chr3.3:75722317-75722336 MS.gene33519:CDS 50.0%
GCGAATGGGCGTAAAGATAG+AGG + chr3.3:75721997-75722016 MS.gene33519:CDS 50.0%
TCATCGCATTGGAGGTGAGT+TGG + chr3.3:75723251-75723270 MS.gene33519:intron 50.0%
TGCCTAGATTTGCGCCGAAA+AGG + chr3.3:75722316-75722335 MS.gene33519:CDS 50.0%
TGTCACTCCAAGTTCCCACA+TGG - chr3.3:75723590-75723609 None:intergenic 50.0%
TTTGCTCACCTGGGCATCTT+GGG + chr3.3:75723175-75723194 MS.gene33519:CDS 50.0%
! ACCCTTTTCGGCGCAAATCT+AGG - chr3.3:75722321-75722340 None:intergenic 50.0%
! ATTCAGGTCACTGGTGGACA+AGG + chr3.3:75722415-75722434 MS.gene33519:CDS 50.0%
! GGGATAGCACCTCCCTTTTA+TGG - chr3.3:75724032-75724051 None:intergenic 50.0%
!! AGACAGGGGCTGGTATTTTC+AGG + chr3.3:75723100-75723119 MS.gene33519:intron 50.0%
!! GTTGGTAGCTCACTGAAGCT+TGG + chr3.3:75723122-75723141 MS.gene33519:CDS 50.0%
!!! AGGGGCTGGTATTTTCAGGT+TGG + chr3.3:75723104-75723123 MS.gene33519:intron 50.0%
CTAGCTAAAGACGAGGGAGC+AGG - chr3.3:75723894-75723913 None:intergenic 55.0%
CTTTGCTCACCTGGGCATCT+TGG + chr3.3:75723174-75723193 MS.gene33519:CDS 55.0%
GTGATGGCCATGTGGGAACT+TGG + chr3.3:75723580-75723599 MS.gene33519:CDS 55.0%
!! GCAAATCTAGGCACCGAACC+AGG - chr3.3:75722309-75722328 None:intergenic 55.0%
GTCACGTGCAGCACCGTCAC+AGG - chr3.3:75723540-75723559 None:intergenic 65.0%
! GGTGCTGCACGTGACGCCTA+TGG + chr3.3:75723545-75723564 MS.gene33519:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 75721847 75724334 75721847 ID=MS.gene33519
chr3.3 mRNA 75721847 75724334 75721847 ID=MS.gene33519.t1;Parent=MS.gene33519
chr3.3 exon 75721847 75722454 75721847 ID=MS.gene33519.t1.exon1;Parent=MS.gene33519.t1
chr3.3 CDS 75721847 75722454 75721847 ID=cds.MS.gene33519.t1;Parent=MS.gene33519.t1
chr3.3 exon 75723106 75723264 75723106 ID=MS.gene33519.t1.exon2;Parent=MS.gene33519.t1
chr3.3 CDS 75723106 75723264 75723106 ID=cds.MS.gene33519.t1;Parent=MS.gene33519.t1
chr3.3 exon 75723422 75723708 75723422 ID=MS.gene33519.t1.exon3;Parent=MS.gene33519.t1
chr3.3 CDS 75723422 75723708 75723422 ID=cds.MS.gene33519.t1;Parent=MS.gene33519.t1
chr3.3 exon 75724135 75724334 75724135 ID=MS.gene33519.t1.exon4;Parent=MS.gene33519.t1
chr3.3 CDS 75724135 75724334 75724135 ID=cds.MS.gene33519.t1;Parent=MS.gene33519.t1
Gene Sequence

>MS.gene33519

AGCTATGTTGAGGCTCTAAAATCCAATGTTTCAAGGGATGCATGTAATGTGTTTGATGGAAGTTGGATTCAGGATGATAGTTATAGTTATAGTTACCCTTTATATGATGCTTCTCAATGTCCATTTGCAGAAAGAGGTTTTAACTGTTTTGCGAATGGGCGTAAAGATAGAGGTTATACAAAATGGAGATGGAAACCTAAGAACTGTGATATTCCTAGGTTTGATGCACGTAGAATACTCGAAAATCTTCGTGGGAAAAGAGTGGTTTTTGTTGGTGATTCATTGAGTAGGACTCAATGGGAGTCTTTGATATGTATGTTAATGACAGGTGTTGAGAATAAGAAGAGTGTTTATGAAATCAAAGGCAATAAGATCACCAAACAAATTAGGTTTCTAGGTGTGAGGTTTAGTTCTTTTGATGTTAGAATTGATTTTTATCGTTCTGTTTTTCTCGTGAAGCCTGGTTCGGTGCCTAGATTTGCGCCGAAAAGGGTGAAGACAACACTGAGATTGGACAAGATAGATGATATTAGCCATGAATGGGTTGATTCAGATGTTCTTATATTCAATTCAGGTCACTGGTGGACAAGGACTAAGCTTTTTGATATGTGAGTAACTTGATTCTACAAATTGAGATGTTTTTTTTTCTTCTTCTACTATGTAGATGATAGATATACATTTTTATGGTCTTATCTTGTTTCACAAATTAGAGATTTGATTTACTACTCTGCATATTTTGTTTACATGCTTTTTTTGGACTTAATTTTCATATTTGGATCCGGGTAATGCATGCTTTACTGTCAGTTTGTTGATCAGAATCTTTATAAGTGATATGAAATTTATGAATTGTAAGAAGGTGGACATCTTATTGTGCTTTTGTGTTCAAAGAATCTTCAACTAGCGTTAGCTTTTCTGCAACTGAAAGCATCATGCATATGATAATTTTGGGTGATCCCTGTAATTCTTTAGATAAAATGATGTATAGCATTGAAACTGACTTGAACTTCAACAAGATGATGTTTTATCAGCATGACTATGATGAAACATGTAAAGTATACTTATTTTATTTCAAATAGTGTCATGAAAAATTGGTCTCTTAGTGGCTAGAATGTGTATTCTTTTGCATATTCATCTATCATAAATTTTTGTTTATGCGTGTGCACATGCATTTTAATAGAATTTCCATGCAGGTTAGGATTAATATTTTTATGAAAATTATCAACTATTTGCTGCTAATTGCTAACATGATTATCAGACAGGGGCTGGTATTTTCAGGTTGGTAGCTCACTGAAGCTTGGAATGCCGATTAATTCAGCTTTCAAAACAGCTTTGCTCACCTGGGCATCTTGGGTAGATAATACAGTTAACACAAACAGAACAAGAGTATTCTTCCGAACTTTCGAATCATCGCATTGGAGGTGAGTTGGTTGTAACCATATTATTCATTTTGTTTTGTCACTTTCCAGATGAGACAATGACAACAAAAAGATGATTAATTTTGCTAATCTTTTGGTAATACTAGAGCTATCAAACTTGATGATAAGACTTAATAAGATGTTTATCTTTTTGTGCCAGTGGACATAACCATAATGCTTGCAAAGTGACTAAAAGGCCTTGGAAGAGAACTATAGGCAAGGACAAAAGTCCAATATCAGATATGATCAAAAGAGTTGTGAGAGATATGAGTGCTCCTGTGACGGTGCTGCACGTGACGCCTATGGATGCATATAGGAGTGATGGCCATGTGGGAACTTGGAGTGACAAACCATCTGTACCTGATTGCAGTCATTGGTGTTTACCTGGTGTTCCTGATATGTGGAATGAAATTCTCTTCTCATACTTGCTACAAAAAGATGAAGCAAGTTAGCATCAAAGCTAGTAGTAACATGTGATGTGTTAGTTAGTGGTTGTATTATTGATTTTCCAATTTCCATATATATTAAACAAGCAAAAGTCATTACTAGTATAACATTTACATGAGTAATTAGGTTATACACTCCTGAAGAGAATGTGACTTGTTGTAACAAAAAAAGAGAGCATGTGACCTGCTCCCTCGTCTTTAGCTAGTAATAATAAATTAAGGTAGTACTTTAAGAAGAACTTGTAAGTAGATTAAATAATATTTCTATCTATGATTATTCCAGCTCAAAGTGTATCCAAATGTGTTGTAACGAAGAGTTTACCATAAAAGGGAGGTGCTATCCCTATTGTAAATAATTGTTCAACAATCATTATCATTAACAAGACTTCTCACCAATTTATAAGTGATTGCTGCTTTGACTTTCCAGTGAACTTAATGCTAAATACTTGCTTAAGTGGTTGCATCACAAAAGGTTCAAGTTGGCAAACCACCAGCATAAATTATGGATCTAGAATTCTCTATAGACGTGCACAGATAAGTATTGGATCTGAGATTCTCTATAGCGATGCATACCGAGCATCATATAATTTTAGTCCAATGGAAGGTATAGAAGGTTGTGGTGGCTAG
Protein sequence

>MS.gene33519.t1

SYVEALKSNVSRDACNVFDGSWIQDDSYSYSYPLYDASQCPFAERGFNCFANGRKDRGYTKWRWKPKNCDIPRFDARRILENLRGKRVVFVGDSLSRTQWESLICMLMTGVENKKSVYEIKGNKITKQIRFLGVRFSSFDVRIDFYRSVFLVKPGSVPRFAPKRVKTTLRLDKIDDISHEWVDSDVLIFNSGHWWTRTKLFDMGWYFQVGSSLKLGMPINSAFKTALLTWASWVDNTVNTNRTRVFFRTFESSHWSGHNHNACKVTKRPWKRTIGKDKSPISDMIKRVVRDMSAPVTVLHVTPMDAYRSDGHVGTWSDKPSVPDCSHWCLPGVPDMWNEILFSYLLQKDEAMNLMLNTCLSGCITKGSSWQTTSINYGSRILYRRAQISIGSEILYSDAYRASYNFSPMEGIEGCGG