Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33713.t1 | XP_003604575.1 | 97.3 | 258 | 7 | 0 | 1 | 258 | 17 | 274 | 5.20E-130 | 473.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33713.t1 | G7JLT9 | 97.3 | 258 | 7 | 0 | 1 | 258 | 17 | 274 | 3.7e-130 | 473.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052188 | MS.gene33713 | 0.810363 | 1.20E-50 | -1.69E-46 |
| MS.gene052340 | MS.gene33713 | 0.819866 | 9.27E-53 | -1.69E-46 |
| MS.gene052485 | MS.gene33713 | 0.815112 | 1.09E-51 | -1.69E-46 |
| MS.gene052784 | MS.gene33713 | 0.935623 | 6.34E-97 | -1.69E-46 |
| MS.gene057823 | MS.gene33713 | 0.803064 | 4.17E-49 | -1.69E-46 |
| MS.gene058007 | MS.gene33713 | 0.832116 | 1.14E-55 | -1.69E-46 |
| MS.gene058928 | MS.gene33713 | 0.803063 | 4.17E-49 | -1.69E-46 |
| MS.gene059366 | MS.gene33713 | 0.804815 | 1.80E-49 | -1.69E-46 |
| MS.gene059688 | MS.gene33713 | 0.809375 | 1.95E-50 | -1.69E-46 |
| MS.gene059692 | MS.gene33713 | 0.81038 | 1.19E-50 | -1.69E-46 |
| MS.gene059695 | MS.gene33713 | 0.806364 | 8.53E-50 | -1.69E-46 |
| MS.gene059807 | MS.gene33713 | 0.805081 | 1.59E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33713.t1 | MTR_4g014670 | 97.287 | 258 | 7 | 0 | 1 | 258 | 17 | 274 | 0.0 | 521 |
| MS.gene33713.t1 | MTR_7g069710 | 46.494 | 271 | 89 | 13 | 1 | 248 | 23 | 260 | 2.02e-51 | 169 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 38 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCTCGAAACTTTCTTCCCT+TGG | 0.318412 | 4.3:+83727426 | MS.gene33713:CDS |
| TCTTTGTCATCAAGTTCATT+AGG | 0.362522 | 4.3:+83726876 | MS.gene33713:CDS |
| GATTCTGATAAATAGCATTC+TGG | 0.384127 | 4.3:-83727341 | None:intergenic |
| CCATTCCTACCAGTATCTTT+CGG | 0.386168 | 4.3:-83726957 | None:intergenic |
| TTGTTCTTCTTCTTCTCCAA+AGG | 0.398255 | 4.3:+83727397 | MS.gene33713:CDS |
| GTCGTGGAGCATGGCAGTAC+AGG | 0.404923 | 4.3:-83727485 | None:intergenic |
| TCTTCCGGACGCAACCGCTT+TGG | 0.410420 | 4.3:+83727250 | MS.gene33713:CDS |
| GAATGCTATTTATCAGAATC+AGG | 0.425031 | 4.3:+83727344 | MS.gene33713:CDS |
| GTTTCGAGACCACATCCCTT+TGG | 0.425269 | 4.3:-83727413 | None:intergenic |
| TGTTCTTCTTCTTCTCCAAA+GGG | 0.425701 | 4.3:+83727398 | MS.gene33713:CDS |
| TCGTGGAAACAAACGATGGC+AGG | 0.437134 | 4.3:-83726994 | None:intergenic |
| CCAGGAAAACCGAAAGATAC+TGG | 0.444180 | 4.3:+83726948 | MS.gene33713:CDS |
| GCAGCCAAAGCGGTTGCGTC+CGG | 0.447146 | 4.3:-83727254 | None:intergenic |
| TCTTCTTCTCCAAAGGGATG+TGG | 0.458927 | 4.3:+83727404 | MS.gene33713:CDS |
| GGTTCTTTCTCCCATGAAAA+CGG | 0.468783 | 4.3:-83726927 | None:intergenic |
| CAGCGCAACTCGAGAGAGTC+GGG | 0.479961 | 4.3:+83727530 | MS.gene33713:CDS |
| CTTTCTTCACGTTCGTGCAA+GGG | 0.499824 | 4.3:-83727583 | None:intergenic |
| TGATCATGTAAGTAGGAGAT+TGG | 0.506183 | 4.3:-83727220 | None:intergenic |
| TCTTTCTTCACGTTCGTGCA+AGG | 0.523409 | 4.3:-83727584 | None:intergenic |
| CAAAGATGTGTCTGCAACGA+CGG | 0.528117 | 4.3:-83726859 | None:intergenic |
| CTGCAGATTTGTCGTAGTCG+TGG | 0.532103 | 4.3:-83727501 | None:intergenic |
| CCGAAAGATACTGGTAGGAA+TGG | 0.534229 | 4.3:+83726957 | MS.gene33713:CDS |
| TGATCTTTACTCAGATGCTA+TGG | 0.539239 | 4.3:+83727085 | MS.gene33713:CDS |
| ATTATGTCAACACGTCGTCT+TGG | 0.552256 | 4.3:+83726834 | None:intergenic |
| TTATGCTTCATAGTTCTCCA+AGG | 0.554836 | 4.3:-83727443 | None:intergenic |
| AAACGGTTGATCATGTAAGT+AGG | 0.561511 | 4.3:-83727227 | None:intergenic |
| TTTGTTTCCACGAAAAGAAG+CGG | 0.584227 | 4.3:+83727004 | MS.gene33713:CDS |
| TCGTGGAGCATGGCAGTACA+GGG | 0.592413 | 4.3:-83727484 | None:intergenic |
| CGTGGAAACAAACGATGGCA+GGG | 0.601100 | 4.3:-83726993 | None:intergenic |
| TATGCTTCATAGTTCTCCAA+GGG | 0.609801 | 4.3:-83727442 | None:intergenic |
| TTAAAGCTTGCAGAGTGTAG+TGG | 0.611037 | 4.3:+83727194 | MS.gene33713:CDS |
| TCAGCGCAACTCGAGAGAGT+CGG | 0.612829 | 4.3:+83727529 | MS.gene33713:CDS |
| GAAAACCGAAAGATACTGGT+AGG | 0.618943 | 4.3:+83726952 | MS.gene33713:CDS |
| TTTGTCGTAGTCGTGGAGCA+TGG | 0.626564 | 4.3:-83727494 | None:intergenic |
| CGGTTGCGTCCGGAAGAAAA+CGG | 0.640456 | 4.3:-83727244 | None:intergenic |
| AGACGATGATGCAGCCAAAG+CGG | 0.673928 | 4.3:-83727264 | None:intergenic |
| AGTCGGGACAGAAGAAACAA+CGG | 0.729959 | 4.3:+83727546 | MS.gene33713:CDS |
| GGAAACAAACGATGGCAGGG+TGG | 0.758125 | 4.3:-83726990 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GATGTAAAAAATGATTGTGA+TGG | + | chr4.3:83727038-83727057 | MS.gene33713:CDS | 25.0% |
| GATTCTGATAAATAGCATTC+TGG | - | chr4.3:83727344-83727363 | None:intergenic | 30.0% | |
| TCTTTGTCATCAAGTTCATT+AGG | + | chr4.3:83726876-83726895 | MS.gene33713:CDS | 30.0% | |
| ! | AAAAAATGATTGTGATGGAG+AGG | + | chr4.3:83727043-83727062 | MS.gene33713:CDS | 30.0% |
| ! | GAATGCTATTTATCAGAATC+AGG | + | chr4.3:83727344-83727363 | MS.gene33713:CDS | 30.0% |
| AAACGGTTGATCATGTAAGT+AGG | - | chr4.3:83727230-83727249 | None:intergenic | 35.0% | |
| TATGCTTCATAGTTCTCCAA+GGG | - | chr4.3:83727445-83727464 | None:intergenic | 35.0% | |
| TGATCATGTAAGTAGGAGAT+TGG | - | chr4.3:83727223-83727242 | None:intergenic | 35.0% | |
| TGATCTTTACTCAGATGCTA+TGG | + | chr4.3:83727085-83727104 | MS.gene33713:CDS | 35.0% | |
| TGTTCTTCTTCTTCTCCAAA+GGG | + | chr4.3:83727398-83727417 | MS.gene33713:CDS | 35.0% | |
| TTATGCTTCATAGTTCTCCA+AGG | - | chr4.3:83727446-83727465 | None:intergenic | 35.0% | |
| TTGTTCTTCTTCTTCTCCAA+AGG | + | chr4.3:83727397-83727416 | MS.gene33713:CDS | 35.0% | |
| TTTGTTTCCACGAAAAGAAG+CGG | + | chr4.3:83727004-83727023 | MS.gene33713:CDS | 35.0% | |
| ! | TGAAAGAGTTCCGTTTTCAT+GGG | + | chr4.3:83726917-83726936 | MS.gene33713:CDS | 35.0% |
| CCATTCCTACCAGTATCTTT+CGG | - | chr4.3:83726960-83726979 | None:intergenic | 40.0% | |
| GAAAACCGAAAGATACTGGT+AGG | + | chr4.3:83726952-83726971 | MS.gene33713:CDS | 40.0% | |
| GGTTCTTTCTCCCATGAAAA+CGG | - | chr4.3:83726930-83726949 | None:intergenic | 40.0% | |
| TTAAAGCTTGCAGAGTGTAG+TGG | + | chr4.3:83727194-83727213 | MS.gene33713:CDS | 40.0% | |
| ! | CATGATCAACCGTTTTCTTC+CGG | + | chr4.3:83727235-83727254 | MS.gene33713:CDS | 40.0% |
| ! | CTTTTCGTGGAAACAAACGA+TGG | - | chr4.3:83727001-83727020 | None:intergenic | 40.0% |
| ! | GTGAAAGAGTTCCGTTTTCA+TGG | + | chr4.3:83726916-83726935 | MS.gene33713:CDS | 40.0% |
| ! | TTTTCATGGGAGAAAGAACC+AGG | + | chr4.3:83726930-83726949 | MS.gene33713:CDS | 40.0% |
| AGTCGGGACAGAAGAAACAA+CGG | + | chr4.3:83727546-83727565 | MS.gene33713:CDS | 45.0% | |
| CAAAGATGTGTCTGCAACGA+CGG | - | chr4.3:83726862-83726881 | None:intergenic | 45.0% | |
| CCAGGAAAACCGAAAGATAC+TGG | + | chr4.3:83726948-83726967 | MS.gene33713:CDS | 45.0% | |
| CCGAAAGATACTGGTAGGAA+TGG | + | chr4.3:83726957-83726976 | MS.gene33713:CDS | 45.0% | |
| CTTTCTTCACGTTCGTGCAA+GGG | - | chr4.3:83727586-83727605 | None:intergenic | 45.0% | |
| GTCTCGAAACTTTCTTCCCT+TGG | + | chr4.3:83727426-83727445 | MS.gene33713:CDS | 45.0% | |
| TCTTCTTCTCCAAAGGGATG+TGG | + | chr4.3:83727404-83727423 | MS.gene33713:CDS | 45.0% | |
| TCTTTCTTCACGTTCGTGCA+AGG | - | chr4.3:83727587-83727606 | None:intergenic | 45.0% | |
| ! | CCAGTATCTTTCGGTTTTCC+TGG | - | chr4.3:83726951-83726970 | None:intergenic | 45.0% |
| AGACGATGATGCAGCCAAAG+CGG | - | chr4.3:83727267-83727286 | None:intergenic | 50.0% | |
| CGTGGAAACAAACGATGGCA+GGG | - | chr4.3:83726996-83727015 | None:intergenic | 50.0% | |
| CTGCAGATTTGTCGTAGTCG+TGG | - | chr4.3:83727504-83727523 | None:intergenic | 50.0% | |
| GTTTCGAGACCACATCCCTT+TGG | - | chr4.3:83727416-83727435 | None:intergenic | 50.0% | |
| TCGTGGAAACAAACGATGGC+AGG | - | chr4.3:83726997-83727016 | None:intergenic | 50.0% | |
| TTTGTCGTAGTCGTGGAGCA+TGG | - | chr4.3:83727497-83727516 | None:intergenic | 50.0% | |
| ! | TGATCAGCCGCTTCTTTTCG+TGG | - | chr4.3:83727014-83727033 | None:intergenic | 50.0% |
| CGGTTGCGTCCGGAAGAAAA+CGG | - | chr4.3:83727247-83727266 | None:intergenic | 55.0% | |
| GGAAACAAACGATGGCAGGG+TGG | - | chr4.3:83726993-83727012 | None:intergenic | 55.0% | |
| TCAGCGCAACTCGAGAGAGT+CGG | + | chr4.3:83727529-83727548 | MS.gene33713:CDS | 55.0% | |
| TCGTGGAGCATGGCAGTACA+GGG | - | chr4.3:83727487-83727506 | None:intergenic | 55.0% | |
| CAGCGCAACTCGAGAGAGTC+GGG | + | chr4.3:83727530-83727549 | MS.gene33713:CDS | 60.0% | |
| GTCGTGGAGCATGGCAGTAC+AGG | - | chr4.3:83727488-83727507 | None:intergenic | 60.0% | |
| TCTTCCGGACGCAACCGCTT+TGG | + | chr4.3:83727250-83727269 | MS.gene33713:CDS | 60.0% | |
| GCAGCCAAAGCGGTTGCGTC+CGG | - | chr4.3:83727257-83727276 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 83726837 | 83727613 | 83726837 | ID=MS.gene33713 |
| chr4.3 | mRNA | 83726837 | 83727613 | 83726837 | ID=MS.gene33713.t1;Parent=MS.gene33713 |
| chr4.3 | exon | 83726837 | 83727613 | 83726837 | ID=MS.gene33713.t1.exon1;Parent=MS.gene33713.t1 |
| chr4.3 | CDS | 83726837 | 83727613 | 83726837 | ID=cds.MS.gene33713.t1;Parent=MS.gene33713.t1 |
| Gene Sequence |
| Protein sequence |
