Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052784.t1 | XP_003604575.1 | 97.7 | 258 | 6 | 0 | 1 | 258 | 17 | 274 | 2.70E-131 | 478 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052784.t1 | G7JLT9 | 97.7 | 258 | 6 | 0 | 1 | 258 | 17 | 274 | 2.0e-131 | 478.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052784 | MS.gene058007 | 0.815322 | 9.81E-52 | -1.69E-46 |
| MS.gene052784 | MS.gene058700 | 0.801393 | 9.20E-49 | -1.69E-46 |
| MS.gene052784 | MS.gene068064 | 0.825319 | 5.00E-54 | -1.69E-46 |
| MS.gene052784 | MS.gene070266 | 0.81107 | 8.41E-51 | -1.69E-46 |
| MS.gene052784 | MS.gene073295 | 0.80472 | 1.89E-49 | -1.69E-46 |
| MS.gene052784 | MS.gene09923 | 0.807903 | 4.03E-50 | -1.69E-46 |
| MS.gene052784 | MS.gene21534 | 0.805049 | 1.61E-49 | -1.69E-46 |
| MS.gene052784 | MS.gene22386 | 0.811015 | 8.65E-51 | -1.69E-46 |
| MS.gene052784 | MS.gene22721 | 0.800046 | 1.73E-48 | -1.69E-46 |
| MS.gene052784 | MS.gene28693 | 0.800112 | 1.68E-48 | -1.69E-46 |
| MS.gene052784 | MS.gene28694 | 0.815774 | 7.78E-52 | -1.69E-46 |
| MS.gene052784 | MS.gene29173 | 0.800024 | 1.75E-48 | -1.69E-46 |
| MS.gene052784 | MS.gene31035 | 0.802752 | 4.83E-49 | -1.69E-46 |
| MS.gene052784 | MS.gene33713 | 0.935623 | 6.34E-97 | -1.69E-46 |
| MS.gene052784 | MS.gene43782 | 0.81142 | 7.06E-51 | -1.69E-46 |
| MS.gene052784 | MS.gene46956 | 0.815665 | 8.23E-52 | -1.69E-46 |
| MS.gene052784 | MS.gene47869 | 0.812434 | 4.25E-51 | -1.69E-46 |
| MS.gene052784 | MS.gene51067 | 0.801756 | 7.75E-49 | -1.69E-46 |
| MS.gene052784 | MS.gene51922 | 0.802074 | 6.67E-49 | -1.69E-46 |
| MS.gene052784 | MS.gene56177 | 0.819759 | 9.81E-53 | -1.69E-46 |
| MS.gene052784 | MS.gene62822 | 0.800507 | 1.40E-48 | -1.69E-46 |
| MS.gene052784 | MS.gene63115 | 0.80751 | 4.88E-50 | -1.69E-46 |
| MS.gene052784 | MS.gene75493 | 0.805954 | 1.04E-49 | -1.69E-46 |
| MS.gene052784 | MS.gene86216 | 0.805221 | 1.48E-49 | -1.69E-46 |
| MS.gene052784 | MS.gene88768 | 0.820726 | 5.89E-53 | -1.69E-46 |
| MS.gene052784 | MS.gene90950 | 0.801686 | 8.01E-49 | -1.69E-46 |
| MS.gene052784 | MS.gene93381 | 0.876075 | 1.86E-68 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052784.t1 | MTR_4g014670 | 97.674 | 258 | 6 | 0 | 1 | 258 | 17 | 274 | 0.0 | 525 |
| MS.gene052784.t1 | MTR_7g069710 | 46.494 | 271 | 89 | 13 | 1 | 248 | 23 | 260 | 1.58e-51 | 169 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 38 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCTCGAAACTTTCTTCCCT+TGG | 0.318412 | 4.4:+86161643 | MS.gene052784:CDS |
| TCTTTGTCATCAAGTTCATT+AGG | 0.362522 | 4.4:+86161093 | MS.gene052784:CDS |
| GATTCTGATAAATAGCATTC+TGG | 0.384127 | 4.4:-86161558 | None:intergenic |
| CCATTCCTACCAGTATCTTT+CGG | 0.386168 | 4.4:-86161174 | None:intergenic |
| TTGTTCTTCTTCTTCTCCAA+AGG | 0.398255 | 4.4:+86161614 | MS.gene052784:CDS |
| GTCGTGGAGCATGGCAGTAC+AGG | 0.404923 | 4.4:-86161702 | None:intergenic |
| TCTTCCGGACGCAACCGCTT+TGG | 0.410420 | 4.4:+86161467 | MS.gene052784:CDS |
| GAATGCTATTTATCAGAATC+AGG | 0.425031 | 4.4:+86161561 | MS.gene052784:CDS |
| GTTTCGAGACCACATCCCTT+TGG | 0.425269 | 4.4:-86161630 | None:intergenic |
| TGTTCTTCTTCTTCTCCAAA+GGG | 0.425701 | 4.4:+86161615 | MS.gene052784:CDS |
| TCGTGGAAACAAACGATGGC+AGG | 0.437134 | 4.4:-86161211 | None:intergenic |
| CCAGGAAAACCGAAAGATAC+TGG | 0.444180 | 4.4:+86161165 | MS.gene052784:CDS |
| GCAGCCAAAGCGGTTGCGTC+CGG | 0.447146 | 4.4:-86161471 | None:intergenic |
| TCTTCTTCTCCAAAGGGATG+TGG | 0.458927 | 4.4:+86161621 | MS.gene052784:CDS |
| GGTTCTTTCTCCCATGAAAA+CGG | 0.468783 | 4.4:-86161144 | None:intergenic |
| CAGCGCAACTCGAGAGAGTC+GGG | 0.479961 | 4.4:+86161747 | MS.gene052784:CDS |
| CTTTCTTCACGTTCGTGCAA+GGG | 0.499824 | 4.4:-86161800 | None:intergenic |
| TGATCATGTAAGTAGGAGAT+TGG | 0.506183 | 4.4:-86161437 | None:intergenic |
| TCTTTCTTCACGTTCGTGCA+AGG | 0.523409 | 4.4:-86161801 | None:intergenic |
| CAAAGATGTGTCTGCAACGA+CGG | 0.528117 | 4.4:-86161076 | None:intergenic |
| CTGCAGATTTGTCGTAGTCG+TGG | 0.532103 | 4.4:-86161718 | None:intergenic |
| CCGAAAGATACTGGTAGGAA+TGG | 0.534229 | 4.4:+86161174 | MS.gene052784:CDS |
| TGATCTTTACTCAGATGCTA+TGG | 0.539239 | 4.4:+86161302 | MS.gene052784:CDS |
| ATTATGTCAACACGTCGTCT+TGG | 0.552256 | 4.4:+86161051 | None:intergenic |
| TTATGCTTCATAGTTCTCCA+AGG | 0.554836 | 4.4:-86161660 | None:intergenic |
| AAACGGTTGATCATGTAAGT+AGG | 0.561511 | 4.4:-86161444 | None:intergenic |
| TTTGTTTCCACGAAAAGAAG+CGG | 0.584227 | 4.4:+86161221 | MS.gene052784:CDS |
| TCGTGGAGCATGGCAGTACA+GGG | 0.592413 | 4.4:-86161701 | None:intergenic |
| CGTGGAAACAAACGATGGCA+GGG | 0.601100 | 4.4:-86161210 | None:intergenic |
| TATGCTTCATAGTTCTCCAA+GGG | 0.609801 | 4.4:-86161659 | None:intergenic |
| TTAAAGCTTGCAGAGTGTAG+TGG | 0.611037 | 4.4:+86161411 | MS.gene052784:CDS |
| TCAGCGCAACTCGAGAGAGT+CGG | 0.612829 | 4.4:+86161746 | MS.gene052784:CDS |
| GAAAACCGAAAGATACTGGT+AGG | 0.618943 | 4.4:+86161169 | MS.gene052784:CDS |
| TTTGTCGTAGTCGTGGAGCA+TGG | 0.626564 | 4.4:-86161711 | None:intergenic |
| CGGTTGCGTCCGGAAGAAAA+CGG | 0.640456 | 4.4:-86161461 | None:intergenic |
| AGACGATGATGCAGCCAAAG+CGG | 0.673928 | 4.4:-86161481 | None:intergenic |
| AGTCGGGACAGAAGAAACAA+CGG | 0.729959 | 4.4:+86161763 | MS.gene052784:CDS |
| GGAAACAAACGATGGCAGGG+TGG | 0.758125 | 4.4:-86161207 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GATCAATCTGATGTAAAAAA+TGG | + | chr4.4:86161246-86161265 | MS.gene052784:CDS | 25.0% |
| GATGTAAAAAATGGTTGTGA+TGG | + | chr4.4:86161255-86161274 | MS.gene052784:CDS | 30.0% | |
| GATTCTGATAAATAGCATTC+TGG | - | chr4.4:86161561-86161580 | None:intergenic | 30.0% | |
| TCTTTGTCATCAAGTTCATT+AGG | + | chr4.4:86161093-86161112 | MS.gene052784:CDS | 30.0% | |
| ! | GAATGCTATTTATCAGAATC+AGG | + | chr4.4:86161561-86161580 | MS.gene052784:CDS | 30.0% |
| AAACGGTTGATCATGTAAGT+AGG | - | chr4.4:86161447-86161466 | None:intergenic | 35.0% | |
| TATGCTTCATAGTTCTCCAA+GGG | - | chr4.4:86161662-86161681 | None:intergenic | 35.0% | |
| TGATCATGTAAGTAGGAGAT+TGG | - | chr4.4:86161440-86161459 | None:intergenic | 35.0% | |
| TGATCTTTACTCAGATGCTA+TGG | + | chr4.4:86161302-86161321 | MS.gene052784:CDS | 35.0% | |
| TGTTCTTCTTCTTCTCCAAA+GGG | + | chr4.4:86161615-86161634 | MS.gene052784:CDS | 35.0% | |
| TTATGCTTCATAGTTCTCCA+AGG | - | chr4.4:86161663-86161682 | None:intergenic | 35.0% | |
| TTGTTCTTCTTCTTCTCCAA+AGG | + | chr4.4:86161614-86161633 | MS.gene052784:CDS | 35.0% | |
| TTTGTTTCCACGAAAAGAAG+CGG | + | chr4.4:86161221-86161240 | MS.gene052784:CDS | 35.0% | |
| ! | AAAAAATGGTTGTGATGGAG+AGG | + | chr4.4:86161260-86161279 | MS.gene052784:CDS | 35.0% |
| ! | TGAAAGAGTTCCGTTTTCAT+GGG | + | chr4.4:86161134-86161153 | MS.gene052784:CDS | 35.0% |
| CCATTCCTACCAGTATCTTT+CGG | - | chr4.4:86161177-86161196 | None:intergenic | 40.0% | |
| GAAAACCGAAAGATACTGGT+AGG | + | chr4.4:86161169-86161188 | MS.gene052784:CDS | 40.0% | |
| GGTTCTTTCTCCCATGAAAA+CGG | - | chr4.4:86161147-86161166 | None:intergenic | 40.0% | |
| TTAAAGCTTGCAGAGTGTAG+TGG | + | chr4.4:86161411-86161430 | MS.gene052784:CDS | 40.0% | |
| ! | CATGATCAACCGTTTTCTTC+CGG | + | chr4.4:86161452-86161471 | MS.gene052784:CDS | 40.0% |
| ! | CTTTTCGTGGAAACAAACGA+TGG | - | chr4.4:86161218-86161237 | None:intergenic | 40.0% |
| ! | GTGAAAGAGTTCCGTTTTCA+TGG | + | chr4.4:86161133-86161152 | MS.gene052784:CDS | 40.0% |
| ! | TTTTCATGGGAGAAAGAACC+AGG | + | chr4.4:86161147-86161166 | MS.gene052784:CDS | 40.0% |
| AGTCGGGACAGAAGAAACAA+CGG | + | chr4.4:86161763-86161782 | MS.gene052784:CDS | 45.0% | |
| CAAAGATGTGTCTGCAACGA+CGG | - | chr4.4:86161079-86161098 | None:intergenic | 45.0% | |
| CCAGGAAAACCGAAAGATAC+TGG | + | chr4.4:86161165-86161184 | MS.gene052784:CDS | 45.0% | |
| CCGAAAGATACTGGTAGGAA+TGG | + | chr4.4:86161174-86161193 | MS.gene052784:CDS | 45.0% | |
| CTTTCTTCACGTTCGTGCAA+GGG | - | chr4.4:86161803-86161822 | None:intergenic | 45.0% | |
| GTCTCGAAACTTTCTTCCCT+TGG | + | chr4.4:86161643-86161662 | MS.gene052784:CDS | 45.0% | |
| TCTTCTTCTCCAAAGGGATG+TGG | + | chr4.4:86161621-86161640 | MS.gene052784:CDS | 45.0% | |
| TCTTTCTTCACGTTCGTGCA+AGG | - | chr4.4:86161804-86161823 | None:intergenic | 45.0% | |
| ! | CCAGTATCTTTCGGTTTTCC+TGG | - | chr4.4:86161168-86161187 | None:intergenic | 45.0% |
| AGACGATGATGCAGCCAAAG+CGG | - | chr4.4:86161484-86161503 | None:intergenic | 50.0% | |
| CGTGGAAACAAACGATGGCA+GGG | - | chr4.4:86161213-86161232 | None:intergenic | 50.0% | |
| CTGCAGATTTGTCGTAGTCG+TGG | - | chr4.4:86161721-86161740 | None:intergenic | 50.0% | |
| GTTTCGAGACCACATCCCTT+TGG | - | chr4.4:86161633-86161652 | None:intergenic | 50.0% | |
| TCGTGGAAACAAACGATGGC+AGG | - | chr4.4:86161214-86161233 | None:intergenic | 50.0% | |
| TTTGTCGTAGTCGTGGAGCA+TGG | - | chr4.4:86161714-86161733 | None:intergenic | 50.0% | |
| ! | TGATCAGCCGCTTCTTTTCG+TGG | - | chr4.4:86161231-86161250 | None:intergenic | 50.0% |
| CGGTTGCGTCCGGAAGAAAA+CGG | - | chr4.4:86161464-86161483 | None:intergenic | 55.0% | |
| GGAAACAAACGATGGCAGGG+TGG | - | chr4.4:86161210-86161229 | None:intergenic | 55.0% | |
| TCAGCGCAACTCGAGAGAGT+CGG | + | chr4.4:86161746-86161765 | MS.gene052784:CDS | 55.0% | |
| TCGTGGAGCATGGCAGTACA+GGG | - | chr4.4:86161704-86161723 | None:intergenic | 55.0% | |
| CAGCGCAACTCGAGAGAGTC+GGG | + | chr4.4:86161747-86161766 | MS.gene052784:CDS | 60.0% | |
| GTCGTGGAGCATGGCAGTAC+AGG | - | chr4.4:86161705-86161724 | None:intergenic | 60.0% | |
| TCTTCCGGACGCAACCGCTT+TGG | + | chr4.4:86161467-86161486 | MS.gene052784:CDS | 60.0% | |
| GCAGCCAAAGCGGTTGCGTC+CGG | - | chr4.4:86161474-86161493 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 86161054 | 86161830 | 86161054 | ID=MS.gene052784 |
| chr4.4 | mRNA | 86161054 | 86161830 | 86161054 | ID=MS.gene052784.t1;Parent=MS.gene052784 |
| chr4.4 | exon | 86161054 | 86161830 | 86161054 | ID=MS.gene052784.t1.exon1;Parent=MS.gene052784.t1 |
| chr4.4 | CDS | 86161054 | 86161830 | 86161054 | ID=cds.MS.gene052784.t1;Parent=MS.gene052784.t1 |
| Gene Sequence |
| Protein sequence |
