Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33829.t1 | KEH31030.1 | 73.4 | 128 | 29 | 1 | 1 | 128 | 10 | 132 | 2.10E-39 | 171.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33829.t1 | Q92346 | 42.7 | 96 | 51 | 3 | 35 | 128 | 2 | 95 | 2.6e-06 | 53.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33829.t1 | A0A072UNS8 | 73.4 | 128 | 29 | 1 | 1 | 128 | 1 | 123 | 1.5e-39 | 171.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050985 | MS.gene33829 | 0.815917 | 7.23E-52 | -1.69E-46 |
| MS.gene057130 | MS.gene33829 | 0.80768 | 4.49E-50 | -1.69E-46 |
| MS.gene057235 | MS.gene33829 | 0.81244 | 4.23E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33829.t1 | MTR_4g088585 | 92.969 | 128 | 4 | 1 | 1 | 128 | 1 | 123 | 2.38e-78 | 227 |
| MS.gene33829.t1 | MTR_4g088585 | 92.969 | 128 | 4 | 1 | 1 | 128 | 10 | 132 | 4.96e-78 | 226 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33829.t1 | AT5G11760 | 62.500 | 40 | 15 | 0 | 89 | 128 | 142 | 181 | 1.96e-13 | 63.9 |
Find 27 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTCTCTTGCAACTTCTTT+GGG | 0.190554 | 4.4:+24254947 | None:intergenic |
| TTGTTCTCTTGCAACTTCTT+TGG | 0.203155 | 4.4:+24254946 | None:intergenic |
| AGGCTTTGGCTCTTCCTTAA+TGG | 0.272980 | 4.4:+24254921 | None:intergenic |
| TCACTCTCATCTAGCCGTTT+TGG | 0.325047 | 4.4:+24254748 | None:intergenic |
| AGGAGTTAGGTCTTAGTAAA+GGG | 0.326348 | 4.4:-24254660 | MS.gene33829:CDS |
| GTGGATTTCTTGTTCGTCAT+TGG | 0.336219 | 4.4:+24254976 | None:intergenic |
| GCAAGAGAACAATACCATTA+AGG | 0.339494 | 4.4:-24254935 | MS.gene33829:CDS |
| TTGGGTGCAGACGAAGGCTT+TGG | 0.398154 | 4.4:+24254907 | None:intergenic |
| GAGGAGTTAGGTCTTAGTAA+AGG | 0.400709 | 4.4:-24254661 | MS.gene33829:CDS |
| AACAATCAAATGGACATAAC+GGG | 0.403236 | 4.4:+24254623 | None:intergenic |
| TAACTCCTCTTCAGTATAGA+TGG | 0.425312 | 4.4:+24254675 | None:intergenic |
| CAACTTCTTTGGGGTTGTAG+TGG | 0.445717 | 4.4:+24254957 | None:intergenic |
| TCAAGAAAGAAAACAGAAGA+TGG | 0.457167 | 4.4:-24254703 | MS.gene33829:CDS |
| ATCTATACTGAAGAGGAGTT+AGG | 0.464087 | 4.4:-24254673 | MS.gene33829:CDS |
| GAACAATCAAATGGACATAA+CGG | 0.471010 | 4.4:+24254622 | None:intergenic |
| GAAATTGACGAAATATTCGC+TGG | 0.474211 | 4.4:-24254871 | MS.gene33829:CDS |
| AAGCAACAAGAACAATCAAA+TGG | 0.475168 | 4.4:+24254613 | None:intergenic |
| GGTCTTAGTAAAGGGGATGC+TGG | 0.495811 | 4.4:-24254652 | MS.gene33829:CDS |
| GGAGTTAGGTCTTAGTAAAG+GGG | 0.507906 | 4.4:-24254659 | MS.gene33829:CDS |
| GCTAGATGAGAGTGAGTTTG+TGG | 0.514984 | 4.4:-24254740 | MS.gene33829:CDS |
| GTTCTCTTGCAACTTCTTTG+GGG | 0.544248 | 4.4:+24254948 | None:intergenic |
| CAAGAAAGAAAACAGAAGAT+GGG | 0.561183 | 4.4:-24254702 | MS.gene33829:CDS |
| TCTTTCTTGATCGAGAAGGC+CGG | 0.574130 | 4.4:+24254716 | None:intergenic |
| GCTTACCATCTATACTGAAG+AGG | 0.624042 | 4.4:-24254680 | MS.gene33829:CDS |
| ATGAGAGTGAGTTTGTGGAC+CGG | 0.626669 | 4.4:-24254735 | MS.gene33829:CDS |
| AAATTGACGAAATATTCGCT+GGG | 0.650459 | 4.4:-24254870 | MS.gene33829:CDS |
| ACAATCAAATGGACATAACG+GGG | 0.706413 | 4.4:+24254624 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGACAAGAAAAAACCAAAA+CGG | - | chr4.4:24254820-24254839 | MS.gene33829:CDS | 25.0% |
| !!! | AATTTCACTAGCTGTTTTTT+TGG | + | chr4.4:24254697-24254716 | None:intergenic | 25.0% |
| !!! | ATTTCACTAGCTGTTTTTTT+GGG | + | chr4.4:24254696-24254715 | None:intergenic | 25.0% |
| AAATCTGATGTGAAAAAGAC+TGG | - | chr4.4:24254747-24254766 | MS.gene33829:CDS | 30.0% | |
| AAATTGACGAAATATTCGCT+GGG | - | chr4.4:24254712-24254731 | MS.gene33829:CDS | 30.0% | |
| AACAATCAAATGGACATAAC+GGG | + | chr4.4:24254962-24254981 | None:intergenic | 30.0% | |
| AAGCAACAAGAACAATCAAA+TGG | + | chr4.4:24254972-24254991 | None:intergenic | 30.0% | |
| CAAGAAAGAAAACAGAAGAT+GGG | - | chr4.4:24254880-24254899 | MS.gene33829:CDS | 30.0% | |
| GAACAATCAAATGGACATAA+CGG | + | chr4.4:24254963-24254982 | None:intergenic | 30.0% | |
| TCAAGAAAGAAAACAGAAGA+TGG | - | chr4.4:24254879-24254898 | MS.gene33829:CDS | 30.0% | |
| !! | ATTACTTTTAGTTGCTTCAC+CGG | + | chr4.4:24254778-24254797 | None:intergenic | 30.0% |
| ACAATCAAATGGACATAACG+GGG | + | chr4.4:24254961-24254980 | None:intergenic | 35.0% | |
| AGGAGTTAGGTCTTAGTAAA+GGG | - | chr4.4:24254922-24254941 | MS.gene33829:CDS | 35.0% | |
| ATCTATACTGAAGAGGAGTT+AGG | - | chr4.4:24254909-24254928 | MS.gene33829:CDS | 35.0% | |
| GAAATTGACGAAATATTCGC+TGG | - | chr4.4:24254711-24254730 | MS.gene33829:CDS | 35.0% | |
| GCAAGAGAACAATACCATTA+AGG | - | chr4.4:24254647-24254666 | MS.gene33829:CDS | 35.0% | |
| TAACTCCTCTTCAGTATAGA+TGG | + | chr4.4:24254910-24254929 | None:intergenic | 35.0% | |
| TGTTCTCTTGCAACTTCTTT+GGG | + | chr4.4:24254638-24254657 | None:intergenic | 35.0% | |
| TTGTTCTCTTGCAACTTCTT+TGG | + | chr4.4:24254639-24254658 | None:intergenic | 35.0% | |
| ! | GTTTTCTTTCTTGATCGAGA+AGG | + | chr4.4:24254873-24254892 | None:intergenic | 35.0% |
| GAGGAGTTAGGTCTTAGTAA+AGG | - | chr4.4:24254921-24254940 | MS.gene33829:CDS | 40.0% | |
| GCTTACCATCTATACTGAAG+AGG | - | chr4.4:24254902-24254921 | MS.gene33829:CDS | 40.0% | |
| GGAGTTAGGTCTTAGTAAAG+GGG | - | chr4.4:24254923-24254942 | MS.gene33829:CDS | 40.0% | |
| GTGAAAAAGACTGGAAAAGC+CGG | - | chr4.4:24254756-24254775 | MS.gene33829:CDS | 40.0% | |
| GTTCTCTTGCAACTTCTTTG+GGG | + | chr4.4:24254637-24254656 | None:intergenic | 40.0% | |
| ATGAGAGTGAGTTTGTGGAC+CGG | - | chr4.4:24254847-24254866 | MS.gene33829:CDS | 45.0% | |
| GCTAGATGAGAGTGAGTTTG+TGG | - | chr4.4:24254842-24254861 | MS.gene33829:CDS | 45.0% | |
| TCTTTCTTGATCGAGAAGGC+CGG | + | chr4.4:24254869-24254888 | None:intergenic | 45.0% | |
| ! | TCACTCTCATCTAGCCGTTT+TGG | + | chr4.4:24254837-24254856 | None:intergenic | 45.0% |
| !! | AGGCTTTGGCTCTTCCTTAA+TGG | + | chr4.4:24254664-24254683 | None:intergenic | 45.0% |
| !! | CAACTTCTTTGGGGTTGTAG+TGG | + | chr4.4:24254628-24254647 | None:intergenic | 45.0% |
| !!! | GTTTTTTTGGGTGCAGACGA+AGG | + | chr4.4:24254684-24254703 | None:intergenic | 45.0% |
| GGTCTTAGTAAAGGGGATGC+TGG | - | chr4.4:24254930-24254949 | MS.gene33829:CDS | 50.0% | |
| TTGGGTGCAGACGAAGGCTT+TGG | + | chr4.4:24254678-24254697 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 24254609 | 24254995 | 24254609 | ID=MS.gene33829 |
| chr4.4 | mRNA | 24254609 | 24254995 | 24254609 | ID=MS.gene33829.t1;Parent=MS.gene33829 |
| chr4.4 | exon | 24254609 | 24254995 | 24254609 | ID=MS.gene33829.t1.exon1;Parent=MS.gene33829.t1 |
| chr4.4 | CDS | 24254609 | 24254995 | 24254609 | ID=cds.MS.gene33829.t1;Parent=MS.gene33829.t1 |
| Gene Sequence |
| Protein sequence |
