AlfalfaGEDB | MS.gene33830

M. sativa cultivar XinJiangDaYe / MS.gene33830

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02701.18

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33830.t1	XP_013456998.1	90.8	348	16	4	10	347	39	380	5.40E-98	367.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33830.t1	Q9LSL6	67.9	81	26	0	7	87	22	102	1.7e-28	128.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33830.t1	A0A072UML9	90.8	348	16	4	10	347	39	380	3.9e-98	367.9

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene33830.t1	TF	C2C2-Dof

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052190	MS.gene33830	0.82537	4.87E-54	-1.69E-46
MS.gene055818	MS.gene33830	0.804966	1.68E-49	-1.69E-46
MS.gene060553	MS.gene33830	0.802133	6.48E-49	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33830.t1	MTR_4g088580	92.737	358	9	5	1	347	29	380	0.0	566
MS.gene33830.t1	MTR_2g014170	41.520	342	127	15	9	343	27	302	5.29e-43	151
MS.gene33830.t1	MTR_8g068210	62.651	83	31	0	17	99	23	105	1.87e-34	129
MS.gene33830.t1	MTR_8g479350	65.432	81	28	0	21	101	63	143	4.66e-33	125
MS.gene33830.t1	MTR_2g096740	76.562	64	15	0	26	89	62	125	5.71e-33	124
MS.gene33830.t1	MTR_2g096740	76.562	64	15	0	26	89	77	140	6.56e-33	124
MS.gene33830.t1	MTR_4g022370	46.032	126	68	0	19	144	75	200	7.66e-33	125
MS.gene33830.t1	MTR_4g063780	62.222	90	30	1	19	104	68	157	1.16e-32	124
MS.gene33830.t1	MTR_2g093220	83.333	60	10	0	21	80	35	94	1.95e-32	123
MS.gene33830.t1	MTR_4g461080	85.965	57	8	0	22	78	25	81	4.92e-32	121

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33830.t1	AT1G28310	38.462	273	130	9	26	291	27	268	1.03e-35	132
MS.gene33830.t1	AT1G28310	38.462	273	130	9	26	291	51	292	1.43e-35	132
MS.gene33830.t1	AT5G65590	67.901	81	26	0	7	87	22	102	3.17e-35	131
MS.gene33830.t1	AT5G60200	81.967	61	11	0	20	80	49	109	2.59e-34	127
MS.gene33830.t1	AT2G37590	65.753	73	25	0	10	82	73	145	1.35e-32	124
MS.gene33830.t1	AT3G61850	45.455	143	59	4	23	146	71	213	1.76e-32	123
MS.gene33830.t1	AT3G61850	44.828	145	61	4	21	146	57	201	2.00e-32	122
MS.gene33830.t1	AT3G61850	44.828	145	61	4	21	146	57	201	2.00e-32	122
MS.gene33830.t1	AT1G07640	46.825	126	63	1	7	132	2	123	4.54e-32	121
MS.gene33830.t1	AT5G02460	82.759	58	10	0	23	80	92	149	1.03e-31	123

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Find 63 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGATATTGGACAAGAGG+TGG	0.582761	4.4:-24260556	MS.gene33830:CDS
AAAGAAGTGAAACTAGGTGA+AGG	0.626336	4.4:-24259917	MS.gene33830:CDS
AACAACAACACTGCAGCAAC+AGG	0.445401	4.4:-24259764	MS.gene33830:CDS
AACAACTACAGTTCCATCTT+TGG	0.305663	4.4:-24260121	MS.gene33830:CDS
AACAGCAACAACAAGCTTTA+AGG	0.128070	4.4:-24260666	MS.gene33830:CDS
AACTGGAGTAGTAGAGGCCA+TGG	0.527816	4.4:+24260069	None:intergenic
AAGAGAATTGATATGGTTTG+AGG	0.411504	4.4:+24260351	None:intergenic
AAGTGGTGGAAATAGGGTTA+TGG	0.446908	4.4:-24260713	MS.gene33830:CDS
AATAATATGAATGCTAATGG+TGG	0.635173	4.4:-24259866	MS.gene33830:CDS
AATAGAAGAAGAACCAAAGA+TGG	0.508474	4.4:+24260108	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGAGAATTGATATGGTTTG+AGG	+	chr4.4:24260073-24260092	None:intergenic	30.0%
AATAGAAGAAGAACCAAAGA+TGG	+	chr4.4:24260316-24260335	None:intergenic	30.0%
ATGTTTGAAGAAGAGTGATT+TGG	+	chr4.4:24259949-24259968	None:intergenic	30.0%
CAGATTCTTCTTCTCTTTAT+TGG	-	chr4.4:24260616-24260635	MS.gene33830:CDS	30.0%
CATCATGATGATTACAGAAA+TGG	-	chr4.4:24260261-24260280	MS.gene33830:CDS	30.0%
GATCACCAACCTAATAATAT+TGG	-	chr4.4:24260702-24260721	MS.gene33830:CDS	30.0%
GATTCAAAAGAAGTGAAACT+AGG	-	chr4.4:24260498-24260517	MS.gene33830:CDS	30.0%
GTAAAGCCTGTAAAAGATAT+TGG	-	chr4.4:24259854-24259873	MS.gene33830:CDS	30.0%
TGTAAAAGATATTGGACAAG+AGG	-	chr4.4:24259862-24259881	MS.gene33830:CDS	30.0%
TTCAACTAATGATAACTCCA+TGG	-	chr4.4:24260335-24260354	MS.gene33830:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	24259697	24260746	24259697	ID=MS.gene33830
chr4.4	mRNA	24259697	24260746	24259697	ID=MS.gene33830.t1;Parent=MS.gene33830
chr4.4	exon	24259697	24260746	24259697	ID=MS.gene33830.t1.exon1;Parent=MS.gene33830.t1
chr4.4	CDS	24259697	24260746	24259697	ID=cds.MS.gene33830.t1;Parent=MS.gene33830.t1

Gene Sequence

>MS.gene33830

ATGGCTTCTTCAAGTGGTGGAAATAGGGTTATGGAAAAACCAGGTCAAGAGCTTCTTCAACAGCAACAACAAGCTTTAAGGTGTCCACGTTGTGATTCATCAAACACTAAATTCTGTTACTACAATAACTACAGTTTATCACAACCAAGACACTTCTGTAAAGCCTGTAAAAGATATTGGACAAGAGGTGGAACACTTAGAAACGTTCCTGTTGGTGGTGGTTGCAGAAAAAACAAACGTCTTAAACGACCAAATCACTCTTCTTCAAACATTGATACTGCTACTGCTTCTCCTTCTTCTTCAACTCCTACTAGTGCTAATTCAAACCCTTCTTCTCAACAACAACAACACCATAGTTTTGATATTGCTTCTACCTCAAACCATATCAATTCTCTTCTTTATGGTAACTCTTCTTGTCATGATGTGATGAACTTTCCTTTTGCAACAAGGTTCAATTCTACTAGAGTTTCAAGTTCTTCTTCTGGTTATGATCATCTTCAGCAGCAGCAGCCTCAGGTGAATGGTCTTGGATTAGGTTTTTCATCTGGGGTAGTTAATCTTAATCATCATGATGATTACAGAAATGGATTTGGTTCAAATAATAACAACTACAGTTCCATCTTTGGTTCTTCTTCTATTTCAACTAATGATAACTCCATGGCCTCTACTACTCCAGTTATGGCTTCTCTACTTAACTCTACACTACTACAACAGAAATTCATGAGTACAGCTGCTGCTGCTGCTGCTGGTGGTGGTGGAATGAAAGGTGGTGATCCTTTTCATCATGAGGCTGGGATAATGGATTCAAAAGAAGTGAAACTAGGTGAAGGACTACAAAATAGGTTGGATCAGTGGAGTAATAATATGAATGCTAATGGTGGTGTTGCTTTTCAAAACCAGATGGAGAACATGGGTTTGTCAGATTCTTCTTCTCTTTATTGGAATAACAACAACACCAACAACAACAACACTGCAGCAACAGGTTTGAGTAGTGTTTGGAGTATTGATCACCAACCTAATAATATTGGTCATAATTCAGTTATTCTCTGA

Protein sequence

>MS.gene33830.t1

MASSSGGNRVMEKPGQELLQQQQQALRCPRCDSSNTKFCYYNNYSLSQPRHFCKACKRYWTRGGTLRNVPVGGGCRKNKRLKRPNHSSSNIDTATASPSSSTPTSANSNPSSQQQQHHSFDIASTSNHINSLLYGNSSCHDVMNFPFATRFNSTRVSSSSSGYDHLQQQQPQVNGLGLGFSSGVVNLNHHDDYRNGFGSNNNNYSSIFGSSSISTNDNSMASTTPVMASLLNSTLLQQKFMSTAAAAAAGGGGMKGGDPFHHEAGIMDSKEVKLGEGLQNRLDQWSNNMNANGGVAFQNQMENMGLSDSSSLYWNNNNTNNNNTAATGLSSVWSIDHQPNNIGHNSVIL