AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33830.t1 MTR_4g088580 92.737 358 9 5 1 347 29 380 0.0 566
MS.gene33830.t1 MTR_2g014170 41.520 342 127 15 9 343 27 302 5.29e-43 151
MS.gene33830.t1 MTR_8g068210 62.651 83 31 0 17 99 23 105 1.87e-34 129
MS.gene33830.t1 MTR_8g479350 65.432 81 28 0 21 101 63 143 4.66e-33 125
MS.gene33830.t1 MTR_2g096740 76.562 64 15 0 26 89 62 125 5.71e-33 124
MS.gene33830.t1 MTR_2g096740 76.562 64 15 0 26 89 77 140 6.56e-33 124
MS.gene33830.t1 MTR_4g022370 46.032 126 68 0 19 144 75 200 7.66e-33 125
MS.gene33830.t1 MTR_4g063780 62.222 90 30 1 19 104 68 157 1.16e-32 124
MS.gene33830.t1 MTR_2g093220 83.333 60 10 0 21 80 35 94 1.95e-32 123
MS.gene33830.t1 MTR_4g461080 85.965 57 8 0 22 78 25 81 4.92e-32 121
MS.gene33830.t1 MTR_8g027295 50.847 118 53 2 15 128 13 129 7.27e-32 120
MS.gene33830.t1 MTR_3g091820 71.233 73 16 1 13 80 25 97 1.20e-31 121
MS.gene33830.t1 MTR_8g079060 65.789 76 26 0 22 97 25 100 1.65e-31 119
MS.gene33830.t1 MTR_3g090430 69.444 72 22 0 11 82 36 107 1.69e-30 119
MS.gene33830.t1 MTR_2g013370 66.667 78 19 2 11 82 25 101 2.26e-30 117
MS.gene33830.t1 MTR_2g014060 77.049 61 14 0 17 77 16 76 2.37e-30 118
MS.gene33830.t1 MTR_4g089095 72.973 74 19 1 10 83 34 106 9.66e-30 115
MS.gene33830.t1 MTR_4g089095 80.000 60 11 1 24 83 14 72 1.19e-29 114
MS.gene33830.t1 MTR_5g031440 79.630 54 11 0 23 76 40 93 1.53e-29 116
MS.gene33830.t1 MTR_7g024670 71.875 64 18 0 25 88 76 139 1.90e-29 116
MS.gene33830.t1 MTR_1g056810 84.906 53 8 0 28 80 14 66 2.82e-29 114
MS.gene33830.t1 MTR_1g077600 67.606 71 21 1 12 80 27 97 3.14e-29 114
MS.gene33830.t1 MTR_1g077600 67.606 71 21 1 12 80 27 97 3.46e-29 114
MS.gene33830.t1 MTR_8g015840 78.571 56 12 0 22 77 16 71 5.71e-29 111
MS.gene33830.t1 MTR_4g109980 81.132 53 10 0 24 76 38 90 1.33e-28 113
MS.gene33830.t1 MTR_3g077750 72.131 61 17 0 16 76 51 111 1.47e-28 113
MS.gene33830.t1 MTR_2g059540 84.000 50 8 0 25 74 30 79 7.63e-27 105
MS.gene33830.t1 MTR_3g435480 65.625 64 22 0 16 79 126 189 1.42e-26 110
MS.gene33830.t1 MTR_4g082060 62.121 66 25 0 16 81 112 177 4.21e-26 108
MS.gene33830.t1 MTR_7g086780 63.077 65 24 0 17 81 90 154 9.77e-26 107
MS.gene33830.t1 MTR_7g010950 65.079 63 22 0 17 79 126 188 1.20e-25 107
MS.gene33830.t1 MTR_6g012450 67.213 61 20 0 19 79 129 189 4.98e-25 105
MS.gene33830.t1 MTR_2g016030 50.000 94 38 2 16 109 37 121 1.29e-24 98.6
MS.gene33830.t1 MTR_6g027450 56.923 65 28 0 17 81 80 144 1.95e-24 102
MS.gene33830.t1 MTR_8g044220 56.923 65 28 0 16 80 108 172 2.60e-24 103
MS.gene33830.t1 MTR_6g027460 58.462 65 27 0 17 81 87 151 3.34e-24 102
MS.gene33830.t1 MTR_5g041420 63.333 60 22 0 20 79 132 191 3.21e-23 98.6
MS.gene33830.t1 MTR_5g041530 64.407 59 21 0 21 79 135 193 2.02e-21 94.4
MS.gene33830.t1 MTR_5g041380 67.857 56 18 0 24 79 125 180 4.18e-21 93.6
MS.gene33830.t1 MTR_5g041400 70.370 54 16 0 26 79 119 172 5.41e-21 93.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33830.t1 AT1G28310 38.462 273 130 9 26 291 27 268 1.03e-35 132
MS.gene33830.t1 AT1G28310 38.462 273 130 9 26 291 51 292 1.43e-35 132
MS.gene33830.t1 AT5G65590 67.901 81 26 0 7 87 22 102 3.17e-35 131
MS.gene33830.t1 AT5G60200 81.967 61 11 0 20 80 49 109 2.59e-34 127
MS.gene33830.t1 AT2G37590 65.753 73 25 0 10 82 73 145 1.35e-32 124
MS.gene33830.t1 AT3G61850 45.455 143 59 4 23 146 71 213 1.76e-32 123
MS.gene33830.t1 AT3G61850 44.828 145 61 4 21 146 57 201 2.00e-32 122
MS.gene33830.t1 AT3G61850 44.828 145 61 4 21 146 57 201 2.00e-32 122
MS.gene33830.t1 AT1G07640 46.825 126 63 1 7 132 2 123 4.54e-32 121
MS.gene33830.t1 AT5G02460 82.759 58 10 0 23 80 92 149 1.03e-31 123
MS.gene33830.t1 AT1G07640 45.255 137 66 2 1 132 47 179 1.82e-31 121
MS.gene33830.t1 AT1G07640 45.255 137 66 2 1 132 55 187 2.43e-31 121
MS.gene33830.t1 AT3G55370 66.667 75 25 0 8 82 58 132 2.53e-31 120
MS.gene33830.t1 AT2G28810 79.310 58 12 0 20 77 71 128 2.69e-31 120
MS.gene33830.t1 AT1G21340 64.557 79 25 1 11 86 20 98 2.94e-31 119
MS.gene33830.t1 AT5G60850 70.667 75 19 1 24 95 49 123 2.98e-31 120
MS.gene33830.t1 AT3G50410 63.218 87 28 3 1 83 1 87 3.14e-31 119
MS.gene33830.t1 AT3G55370 66.667 75 25 0 8 82 58 132 3.32e-31 121
MS.gene33830.t1 AT2G28810 79.310 58 12 0 20 77 88 145 3.55e-31 120
MS.gene33830.t1 AT3G55370 66.667 75 25 0 8 82 103 177 4.13e-31 121
MS.gene33830.t1 AT3G45610 77.586 58 13 0 23 80 37 94 6.50e-31 117
MS.gene33830.t1 AT2G28510 82.456 57 10 0 24 80 46 102 7.86e-31 118
MS.gene33830.t1 AT3G61850 52.778 108 41 2 21 118 57 164 8.08e-31 119
MS.gene33830.t1 AT3G61850 53.774 106 39 2 23 118 71 176 1.05e-30 119
MS.gene33830.t1 AT1G51700 76.923 65 12 2 17 80 26 88 1.48e-30 115
MS.gene33830.t1 AT3G52440 81.818 55 10 0 28 82 27 81 1.48e-29 114
MS.gene33830.t1 AT3G52440 81.818 55 10 0 28 82 46 100 2.12e-29 114
MS.gene33830.t1 AT3G21270 84.906 53 8 0 26 78 29 81 3.64e-29 112
MS.gene33830.t1 AT4G00940 74.603 63 16 0 23 85 65 127 4.22e-29 114
MS.gene33830.t1 AT4G00940 74.603 63 16 0 23 85 65 127 4.22e-29 114
MS.gene33830.t1 AT4G00940 74.603 63 16 0 23 85 65 127 4.22e-29 114
MS.gene33830.t1 AT4G24060 75.000 60 15 0 21 80 48 107 4.45e-29 115
MS.gene33830.t1 AT4G21050 66.197 71 24 0 28 98 26 96 1.24e-28 110
MS.gene33830.t1 AT5G66940 56.977 86 33 2 22 104 28 112 1.48e-28 110
MS.gene33830.t1 AT4G38000 79.310 58 11 1 23 79 38 95 4.87e-28 110
MS.gene33830.t1 AT5G62940 69.355 62 19 0 15 76 62 123 2.51e-27 110
MS.gene33830.t1 AT3G47500 66.667 66 22 0 14 79 98 163 1.46e-26 109
MS.gene33830.t1 AT5G62430 67.213 61 20 0 19 79 47 107 9.76e-26 105
MS.gene33830.t1 AT1G64620 74.545 55 14 0 21 75 44 98 1.78e-25 105
MS.gene33830.t1 AT2G46590 78.431 51 11 0 23 73 65 115 2.01e-25 105
MS.gene33830.t1 AT5G39660 68.852 61 19 0 19 79 131 191 2.36e-25 106
MS.gene33830.t1 AT5G39660 68.852 61 19 0 19 79 131 191 2.36e-25 106
MS.gene33830.t1 AT5G39660 68.852 61 19 0 19 79 131 191 2.36e-25 106
MS.gene33830.t1 AT2G46590 78.431 51 11 0 23 73 77 127 2.39e-25 105
MS.gene33830.t1 AT1G26790 63.934 61 22 0 19 79 104 164 1.42e-24 103
MS.gene33830.t1 AT1G69570 60.294 68 27 0 19 86 125 192 1.91e-24 103
MS.gene33830.t1 AT1G47655 72.549 51 14 0 24 74 27 77 4.76e-24 98.6
MS.gene33830.t1 AT1G29160 56.338 71 29 1 11 79 45 115 1.33e-23 96.3
MS.gene33830.t1 AT4G21040 57.143 77 30 2 28 103 27 101 1.91e-23 97.4
MS.gene33830.t1 AT2G34140 63.333 60 22 0 20 79 52 111 2.35e-23 95.5
MS.gene33830.t1 AT4G21080 65.517 58 19 1 28 84 27 84 2.01e-21 92.4
MS.gene33830.t1 AT4G21030 60.000 50 20 0 28 77 23 72 2.73e-14 71.2

Find 63 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATATCAATTCTCTTCTTTA+TGG 0.060643 4.4:-24260343 MS.gene33830:CDS
CAGATTCTTCTTCTCTTTAT+TGG 0.115189 4.4:-24259805 MS.gene33830:CDS
AACAGCAACAACAAGCTTTA+AGG 0.128070 4.4:-24260666 MS.gene33830:CDS
CTTCTTCAAGTGGTGGAAAT+AGG 0.186986 4.4:-24260720 MS.gene33830:CDS
GAATTATGACCAATATTATT+AGG 0.234994 4.4:+24259710 None:intergenic
CAGGTGAATGGTCTTGGATT+AGG 0.278693 4.4:-24260211 MS.gene33830:CDS
CATAAAGAAGAGAATTGATA+TGG 0.297144 4.4:+24260344 None:intergenic
AACAACTACAGTTCCATCTT+TGG 0.305663 4.4:-24260121 MS.gene33830:CDS
TCATCATGAGGCTGGGATAA+TGG 0.306350 4.4:-24259945 MS.gene33830:CDS
TGCTGCAGTGTTGTTGTTGT+TGG 0.309230 4.4:+24259769 None:intergenic
GTAAAGCCTGTAAAAGATAT+TGG 0.311115 4.4:-24260567 MS.gene33830:CDS
GCTGCTGCTGCTGGTGGTGG+TGG 0.341313 4.4:-24259989 MS.gene33830:CDS
GATGATTACAGAAATGGATT+TGG 0.345610 4.4:-24260154 MS.gene33830:CDS
CATCATGATGATTACAGAAA+TGG 0.360895 4.4:-24260160 MS.gene33830:CDS
CAACAGGTTTGAGTAGTGTT+TGG 0.362182 4.4:-24259748 MS.gene33830:CDS
AGAGTTTCAAGTTCTTCTTC+TGG 0.369826 4.4:-24260262 MS.gene33830:CDS
TTCTTCAAGTGGTGGAAATA+GGG 0.372187 4.4:-24260719 MS.gene33830:CDS
TTAAGTAGAGAAGCCATAAC+TGG 0.397152 4.4:+24260052 None:intergenic
CAGCCTCAGGTGAATGGTCT+TGG 0.403606 4.4:-24260217 MS.gene33830:CDS
AAGAGAATTGATATGGTTTG+AGG 0.411504 4.4:+24260351 None:intergenic
GATCACCAACCTAATAATAT+TGG 0.414706 4.4:-24259719 MS.gene33830:CDS
TAGAAGCAATATCAAAACTA+TGG 0.419426 4.4:+24260374 None:intergenic
ATGTTTGAAGAAGAGTGATT+TGG 0.420876 4.4:+24260475 None:intergenic
AACAACAACACTGCAGCAAC+AGG 0.445401 4.4:-24259764 MS.gene33830:CDS
AAGTGGTGGAAATAGGGTTA+TGG 0.446908 4.4:-24260713 MS.gene33830:CDS
ACAAACCCATGTTCTCCATC+TGG 0.452753 4.4:+24259828 None:intergenic
TAGGTGAAGGACTACAAAAT+AGG 0.461358 4.4:-24259904 MS.gene33830:CDS
GGCCTCTACTACTCCAGTTA+TGG 0.463925 4.4:-24260065 MS.gene33830:CDS
GGGTTTGAATTAGCACTAGT+AGG 0.464115 4.4:+24260418 None:intergenic
GAATTGAACCTTGTTGCAAA+AGG 0.464424 4.4:+24260289 None:intergenic
TATGACCAATATTATTAGGT+TGG 0.469646 4.4:+24259714 None:intergenic
TTTCTGCAACCACCACCAAC+AGG 0.472277 4.4:+24260517 None:intergenic
GCTGGTGGTGGTGGAATGAA+AGG 0.473306 4.4:-24259980 MS.gene33830:CDS
ATCCCAGCCTCATGATGAAA+AGG 0.481394 4.4:+24259950 None:intergenic
CAGGCTTTACAGAAGTGTCT+TGG 0.500398 4.4:+24260580 None:intergenic
TGTTGTTGTTGTTGAGAAGA+AGG 0.501373 4.4:+24260397 None:intergenic
GTTGTTGTTGTTGAGAAGAA+GGG 0.502011 4.4:+24260398 None:intergenic
AATAGAAGAAGAACCAAAGA+TGG 0.508474 4.4:+24260108 None:intergenic
TCAAAACCAGATGGAGAACA+TGG 0.510931 4.4:-24259834 MS.gene33830:CDS
CAGCAGCAGCCTCAGGTGAA+TGG 0.518167 4.4:-24260223 MS.gene33830:CDS
CTTAGAAACGTTCCTGTTGG+TGG 0.520182 4.4:-24260529 MS.gene33830:CDS
AGTAATAATATGAATGCTAA+TGG 0.525718 4.4:-24259869 MS.gene33830:CDS
AACTGGAGTAGTAGAGGCCA+TGG 0.527816 4.4:+24260069 None:intergenic
TGAAGGACTACAAAATAGGT+TGG 0.535498 4.4:-24259900 MS.gene33830:CDS
CTAGTAGGAGTTGAAGAAGA+AGG 0.548358 4.4:+24260433 None:intergenic
TCTTCAGCAGCAGCAGCCTC+AGG 0.551095 4.4:-24260230 MS.gene33830:CDS
TGTAAAAGATATTGGACAAG+AGG 0.561250 4.4:-24260559 MS.gene33830:CDS
GTTTCAATGGCTTCTTCAAG+TGG 0.563411 4.4:-24260730 None:intergenic
AGCCATAACTGGAGTAGTAG+AGG 0.573379 4.4:+24260063 None:intergenic
AGAAACGTTCCTGTTGGTGG+TGG 0.578028 4.4:-24260526 MS.gene33830:CDS
TGTTGAAGAAGCTCTTGACC+TGG 0.579160 4.4:+24260685 None:intergenic
TACAAAATAGGTTGGATCAG+TGG 0.582074 4.4:-24259892 MS.gene33830:CDS
AAAAGATATTGGACAAGAGG+TGG 0.582761 4.4:-24260556 MS.gene33830:CDS
TCAATGGCTTCTTCAAGTGG+TGG 0.584214 4.4:-24260727 MS.gene33830:CDS
GGTGGTGGTGGAATGAAAGG+TGG 0.591912 4.4:-24259977 MS.gene33830:CDS
GATTCAAAAGAAGTGAAACT+AGG 0.595250 4.4:-24259923 MS.gene33830:CDS
TTCAACTAATGATAACTCCA+TGG 0.599304 4.4:-24260086 MS.gene33830:CDS
CAAAACCAGATGGAGAACAT+GGG 0.602211 4.4:-24259833 MS.gene33830:CDS
ACACTTAGAAACGTTCCTGT+TGG 0.611337 4.4:-24260532 MS.gene33830:CDS
AAAGAAGTGAAACTAGGTGA+AGG 0.626336 4.4:-24259917 MS.gene33830:CDS
AATAATATGAATGCTAATGG+TGG 0.635173 4.4:-24259866 MS.gene33830:CDS
AATCCAAGACCATTCACCTG+AGG 0.720277 4.4:+24260214 None:intergenic
GTGTTTGATGAATCACAACG+TGG 0.749662 4.4:+24260640 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTAATAATATGAATGCTAA+TGG - chr4.4:24260552-24260571 MS.gene33830:CDS 20.0%
!! GAATTATGACCAATATTATT+AGG + chr4.4:24260714-24260733 None:intergenic 20.0%
! AATAATATGAATGCTAATGG+TGG - chr4.4:24260555-24260574 MS.gene33830:CDS 25.0%
! CATAAAGAAGAGAATTGATA+TGG + chr4.4:24260080-24260099 None:intergenic 25.0%
! CATATCAATTCTCTTCTTTA+TGG - chr4.4:24260078-24260097 MS.gene33830:CDS 25.0%
! TAGAAGCAATATCAAAACTA+TGG + chr4.4:24260050-24260069 None:intergenic 25.0%
! TATGACCAATATTATTAGGT+TGG + chr4.4:24260710-24260729 None:intergenic 25.0%
AAGAGAATTGATATGGTTTG+AGG + chr4.4:24260073-24260092 None:intergenic 30.0%
AATAGAAGAAGAACCAAAGA+TGG + chr4.4:24260316-24260335 None:intergenic 30.0%
ATGTTTGAAGAAGAGTGATT+TGG + chr4.4:24259949-24259968 None:intergenic 30.0%
CAGATTCTTCTTCTCTTTAT+TGG - chr4.4:24260616-24260635 MS.gene33830:CDS 30.0%
CATCATGATGATTACAGAAA+TGG - chr4.4:24260261-24260280 MS.gene33830:CDS 30.0%
GATCACCAACCTAATAATAT+TGG - chr4.4:24260702-24260721 MS.gene33830:CDS 30.0%
GATTCAAAAGAAGTGAAACT+AGG - chr4.4:24260498-24260517 MS.gene33830:CDS 30.0%
GTAAAGCCTGTAAAAGATAT+TGG - chr4.4:24259854-24259873 MS.gene33830:CDS 30.0%
TGTAAAAGATATTGGACAAG+AGG - chr4.4:24259862-24259881 MS.gene33830:CDS 30.0%
TTCAACTAATGATAACTCCA+TGG - chr4.4:24260335-24260354 MS.gene33830:CDS 30.0%
! GATGATTACAGAAATGGATT+TGG - chr4.4:24260267-24260286 MS.gene33830:CDS 30.0%
! TCTTGTCCAATATCTTTTAC+AGG + chr4.4:24259863-24259882 None:intergenic 30.0%
!! TTGGATTAGGTTTTTCATCT+GGG - chr4.4:24260223-24260242 MS.gene33830:CDS 30.0%
AAAAGATATTGGACAAGAGG+TGG - chr4.4:24259865-24259884 MS.gene33830:CDS 35.0%
AAAGAAGTGAAACTAGGTGA+AGG - chr4.4:24260504-24260523 MS.gene33830:CDS 35.0%
AACAACTACAGTTCCATCTT+TGG - chr4.4:24260300-24260319 MS.gene33830:CDS 35.0%
AACAGCAACAACAAGCTTTA+AGG - chr4.4:24259755-24259774 MS.gene33830:CDS 35.0%
AATAGGGTTATGGAAAAACC+AGG - chr4.4:24259718-24259737 MS.gene33830:CDS 35.0%
GAATTGAACCTTGTTGCAAA+AGG + chr4.4:24260135-24260154 None:intergenic 35.0%
TAGGTGAAGGACTACAAAAT+AGG - chr4.4:24260517-24260536 MS.gene33830:CDS 35.0%
TGAAGGACTACAAAATAGGT+TGG - chr4.4:24260521-24260540 MS.gene33830:CDS 35.0%
TTAAGTAGAGAAGCCATAAC+TGG + chr4.4:24260372-24260391 None:intergenic 35.0%
! GTTGTTGTTGTTGAGAAGAA+GGG + chr4.4:24260026-24260045 None:intergenic 35.0%
! TACAAAATAGGTTGGATCAG+TGG - chr4.4:24260529-24260548 MS.gene33830:CDS 35.0%
! TGAACTTTCCTTTTGCAACA+AGG - chr4.4:24260124-24260143 MS.gene33830:CDS 35.0%
! TGTTGTTGTTGTTGAGAAGA+AGG + chr4.4:24260027-24260046 None:intergenic 35.0%
! TTCTTCAAGTGGTGGAAATA+GGG - chr4.4:24259702-24259721 MS.gene33830:CDS 35.0%
!! AGAGTTTCAAGTTCTTCTTC+TGG - chr4.4:24260159-24260178 MS.gene33830:CDS 35.0%
!! CTTGGATTAGGTTTTTCATC+TGG - chr4.4:24260222-24260241 MS.gene33830:CDS 35.0%
!! TGGATTAGGTTTTTCATCTG+GGG - chr4.4:24260224-24260243 MS.gene33830:CDS 35.0%
!!! TGTTGCTTTTCAAAACCAGA+TGG - chr4.4:24260578-24260597 MS.gene33830:CDS 35.0%
ACACTTAGAAACGTTCCTGT+TGG - chr4.4:24259889-24259908 MS.gene33830:CDS 40.0%
CAAAACCAGATGGAGAACAT+GGG - chr4.4:24260588-24260607 MS.gene33830:CDS 40.0%
CAACAGGTTTGAGTAGTGTT+TGG - chr4.4:24260673-24260692 MS.gene33830:CDS 40.0%
CTAGTAGGAGTTGAAGAAGA+AGG + chr4.4:24259991-24260010 None:intergenic 40.0%
GGGTTTGAATTAGCACTAGT+AGG + chr4.4:24260006-24260025 None:intergenic 40.0%
GTGTTTGATGAATCACAACG+TGG + chr4.4:24259784-24259803 None:intergenic 40.0%
TCAAAACCAGATGGAGAACA+TGG - chr4.4:24260587-24260606 MS.gene33830:CDS 40.0%
! AAGTGGTGGAAATAGGGTTA+TGG - chr4.4:24259708-24259727 MS.gene33830:CDS 40.0%
! ATCCTTTTCATCATGAGGCT+GGG - chr4.4:24260469-24260488 MS.gene33830:CDS 40.0%
! CTTCTTCAAGTGGTGGAAAT+AGG - chr4.4:24259701-24259720 MS.gene33830:CDS 40.0%
! TGGTGATCCTTTTCATCATG+AGG - chr4.4:24260464-24260483 MS.gene33830:CDS 40.0%
AACAACAACACTGCAGCAAC+AGG - chr4.4:24260657-24260676 MS.gene33830:CDS 45.0%
AATCCAAGACCATTCACCTG+AGG + chr4.4:24260210-24260229 None:intergenic 45.0%
ACAAACCCATGTTCTCCATC+TGG + chr4.4:24260596-24260615 None:intergenic 45.0%
AGCCATAACTGGAGTAGTAG+AGG + chr4.4:24260361-24260380 None:intergenic 45.0%
ATCCCAGCCTCATGATGAAA+AGG + chr4.4:24260474-24260493 None:intergenic 45.0%
CTTAGAAACGTTCCTGTTGG+TGG - chr4.4:24259892-24259911 MS.gene33830:CDS 45.0%
TCATCATGAGGCTGGGATAA+TGG - chr4.4:24260476-24260495 MS.gene33830:CDS 45.0%
TGTTGAAGAAGCTCTTGACC+TGG + chr4.4:24259739-24259758 None:intergenic 45.0%
! CAGGTGAATGGTCTTGGATT+AGG - chr4.4:24260210-24260229 MS.gene33830:CDS 45.0%
! GATCCTTTTCATCATGAGGC+TGG - chr4.4:24260468-24260487 MS.gene33830:CDS 45.0%
! TGCTGCAGTGTTGTTGTTGT+TGG + chr4.4:24260655-24260674 None:intergenic 45.0%
!! CAGGCTTTACAGAAGTGTCT+TGG + chr4.4:24259844-24259863 None:intergenic 45.0%
AACTGGAGTAGTAGAGGCCA+TGG + chr4.4:24260355-24260374 None:intergenic 50.0%
GGCCTCTACTACTCCAGTTA+TGG - chr4.4:24260356-24260375 MS.gene33830:CDS 50.0%
TTTCTGCAACCACCACCAAC+AGG + chr4.4:24259907-24259926 None:intergenic 50.0%
! AGAAACGTTCCTGTTGGTGG+TGG - chr4.4:24259895-24259914 MS.gene33830:CDS 50.0%
CAGCCTCAGGTGAATGGTCT+TGG - chr4.4:24260204-24260223 MS.gene33830:CDS 55.0%
!! GCTGGTGGTGGTGGAATGAA+AGG - chr4.4:24260441-24260460 MS.gene33830:CDS 55.0%
!! GGTGGTGGTGGAATGAAAGG+TGG - chr4.4:24260444-24260463 MS.gene33830:CDS 55.0%
CAGCAGCAGCCTCAGGTGAA+TGG - chr4.4:24260198-24260217 MS.gene33830:CDS 60.0%
TCTTCAGCAGCAGCAGCCTC+AGG - chr4.4:24260191-24260210 MS.gene33830:CDS 60.0%
ACAGCTGCTGCTGCTGCTGC+TGG - chr4.4:24260423-24260442 MS.gene33830:CDS 65.0%
GCTGCTGCTGCTGCTGCTGG+TGG - chr4.4:24260426-24260445 MS.gene33830:CDS 70.0%
GCTGCTGCTGCTGCTGGTGG+TGG - chr4.4:24260429-24260448 MS.gene33830:CDS 70.0%
!! GCTGCTGCTGCTGGTGGTGG+TGG - chr4.4:24260432-24260451 MS.gene33830:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 24259697 24260746 24259697 ID=MS.gene33830
chr4.4 mRNA 24259697 24260746 24259697 ID=MS.gene33830.t1;Parent=MS.gene33830
chr4.4 exon 24259697 24260746 24259697 ID=MS.gene33830.t1.exon1;Parent=MS.gene33830.t1
chr4.4 CDS 24259697 24260746 24259697 ID=cds.MS.gene33830.t1;Parent=MS.gene33830.t1
Gene Sequence

>MS.gene33830

ATGGCTTCTTCAAGTGGTGGAAATAGGGTTATGGAAAAACCAGGTCAAGAGCTTCTTCAACAGCAACAACAAGCTTTAAGGTGTCCACGTTGTGATTCATCAAACACTAAATTCTGTTACTACAATAACTACAGTTTATCACAACCAAGACACTTCTGTAAAGCCTGTAAAAGATATTGGACAAGAGGTGGAACACTTAGAAACGTTCCTGTTGGTGGTGGTTGCAGAAAAAACAAACGTCTTAAACGACCAAATCACTCTTCTTCAAACATTGATACTGCTACTGCTTCTCCTTCTTCTTCAACTCCTACTAGTGCTAATTCAAACCCTTCTTCTCAACAACAACAACACCATAGTTTTGATATTGCTTCTACCTCAAACCATATCAATTCTCTTCTTTATGGTAACTCTTCTTGTCATGATGTGATGAACTTTCCTTTTGCAACAAGGTTCAATTCTACTAGAGTTTCAAGTTCTTCTTCTGGTTATGATCATCTTCAGCAGCAGCAGCCTCAGGTGAATGGTCTTGGATTAGGTTTTTCATCTGGGGTAGTTAATCTTAATCATCATGATGATTACAGAAATGGATTTGGTTCAAATAATAACAACTACAGTTCCATCTTTGGTTCTTCTTCTATTTCAACTAATGATAACTCCATGGCCTCTACTACTCCAGTTATGGCTTCTCTACTTAACTCTACACTACTACAACAGAAATTCATGAGTACAGCTGCTGCTGCTGCTGCTGGTGGTGGTGGAATGAAAGGTGGTGATCCTTTTCATCATGAGGCTGGGATAATGGATTCAAAAGAAGTGAAACTAGGTGAAGGACTACAAAATAGGTTGGATCAGTGGAGTAATAATATGAATGCTAATGGTGGTGTTGCTTTTCAAAACCAGATGGAGAACATGGGTTTGTCAGATTCTTCTTCTCTTTATTGGAATAACAACAACACCAACAACAACAACACTGCAGCAACAGGTTTGAGTAGTGTTTGGAGTATTGATCACCAACCTAATAATATTGGTCATAATTCAGTTATTCTCTGA
Protein sequence

>MS.gene33830.t1

MASSSGGNRVMEKPGQELLQQQQQALRCPRCDSSNTKFCYYNNYSLSQPRHFCKACKRYWTRGGTLRNVPVGGGCRKNKRLKRPNHSSSNIDTATASPSSSTPTSANSNPSSQQQQHHSFDIASTSNHINSLLYGNSSCHDVMNFPFATRFNSTRVSSSSSGYDHLQQQQPQVNGLGLGFSSGVVNLNHHDDYRNGFGSNNNNYSSIFGSSSISTNDNSMASTTPVMASLLNSTLLQQKFMSTAAAAAAGGGGMKGGDPFHHEAGIMDSKEVKLGEGLQNRLDQWSNNMNANGGVAFQNQMENMGLSDSSSLYWNNNNTNNNNTAATGLSSVWSIDHQPNNIGHNSVIL