AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33874

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33874.t1 MTR_1g026830 95.093 591 29 0 1 591 1 591 0.0 1151
MS.gene33874.t1 MTR_1g095810 93.437 579 37 1 14 591 11 589 0.0 1111
MS.gene33874.t1 MTR_2g100230 40.191 418 237 5 117 531 66 473 7.16e-99 310
MS.gene33874.t1 MTR_4g105100 40.648 401 225 5 134 531 189 579 6.42e-92 296
MS.gene33874.t1 MTR_3g083690 38.257 413 229 6 136 531 134 537 1.46e-91 294
MS.gene33874.t1 MTR_3g467550 37.376 404 231 5 111 500 77 472 2.62e-91 292
MS.gene33874.t1 MTR_7g109720 43.544 395 208 7 117 506 202 586 3.72e-91 298
MS.gene33874.t1 MTR_6g005550 39.173 411 232 7 126 531 144 541 4.15e-90 293
MS.gene33874.t1 MTR_1g054630 39.066 407 227 7 136 528 143 542 2.02e-89 288
MS.gene33874.t1 MTR_3g467550 37.250 400 206 6 111 500 77 441 6.26e-87 280
MS.gene33874.t1 MTR_4g134170 35.514 428 251 4 120 529 260 680 1.23e-85 281
MS.gene33874.t1 MTR_4g114120 37.561 410 232 6 138 530 131 533 4.02e-84 276
MS.gene33874.t1 MTR_6g005320 35.417 432 252 5 116 529 218 640 8.68e-84 275
MS.gene33874.t1 MTR_2g032630 39.563 412 231 7 125 531 474 872 3.63e-82 280
MS.gene33874.t1 MTR_2g007000 38.208 424 243 6 117 533 474 885 4.19e-82 279
MS.gene33874.t1 MTR_3g467550 35.356 379 223 5 111 475 77 447 4.82e-77 251
MS.gene33874.t1 MTR_5g027840 37.468 395 230 8 138 525 95 479 6.63e-77 253
MS.gene33874.t1 MTR_4g105100 39.879 331 187 4 134 461 189 510 5.57e-70 235
MS.gene33874.t1 MTR_4g079320 33.498 406 246 7 148 543 20 411 1.40e-63 223
MS.gene33874.t1 MTR_5g090120 37.151 358 206 7 157 508 91 435 7.70e-63 218
MS.gene33874.t1 MTR_6g082080 33.243 370 223 6 145 507 8 360 1.22e-62 213
MS.gene33874.t1 MTR_7g056830 33.243 370 223 6 145 507 8 360 1.43e-62 213
MS.gene33874.t1 MTR_3g449250 36.592 358 204 8 157 506 100 442 2.19e-61 213
MS.gene33874.t1 MTR_3g449250 36.592 358 204 8 157 506 100 442 4.96e-61 213
MS.gene33874.t1 MTR_6g033070 30.913 427 278 9 126 548 120 533 3.66e-60 209
MS.gene33874.t1 MTR_3g049990 35.068 365 217 6 147 504 152 503 5.85e-60 213
MS.gene33874.t1 MTR_7g100820 32.530 415 251 8 127 531 110 505 8.74e-59 207
MS.gene33874.t1 MTR_2g041140 33.795 361 224 4 147 502 141 491 2.79e-57 205
MS.gene33874.t1 MTR_2g039960 31.362 389 248 8 147 532 41 413 4.48e-56 195
MS.gene33874.t1 MTR_2g066050 33.604 369 216 7 147 504 53 403 4.99e-56 197
MS.gene33874.t1 MTR_4g125970 31.362 389 248 8 147 532 41 413 8.50e-56 194
MS.gene33874.t1 MTR_4g125970 31.362 389 248 8 147 532 58 430 2.20e-55 193
MS.gene33874.t1 MTR_2g438110 31.378 392 250 7 144 532 31 406 6.39e-55 191
MS.gene33874.t1 MTR_2g099620 31.026 390 250 8 146 532 40 413 2.89e-54 190
MS.gene33874.t1 MTR_2g099620 31.026 390 250 8 146 532 24 397 2.96e-54 189
MS.gene33874.t1 MTR_8g018400 30.077 389 253 8 147 532 41 413 2.19e-53 187
MS.gene33874.t1 MTR_4g112340 35.030 334 198 8 165 490 115 437 1.06e-52 190
MS.gene33874.t1 MTR_2g055850 31.044 364 236 6 144 501 88 442 3.84e-52 187
MS.gene33874.t1 MTR_3g071620 32.275 378 224 11 148 501 15 384 7.26e-51 185
MS.gene33874.t1 MTR_7g076820 28.704 432 276 8 104 532 118 520 4.70e-50 181
MS.gene33874.t1 MTR_2g043360 28.125 416 219 8 165 507 60 468 1.29e-49 182
MS.gene33874.t1 MTR_7g076800 29.723 397 259 7 139 532 132 511 2.19e-49 179
MS.gene33874.t1 MTR_7g076830 27.982 436 274 8 104 532 111 513 2.48e-49 179
MS.gene33874.t1 MTR_6g056080 33.062 369 223 9 139 497 98 452 3.20e-49 182
MS.gene33874.t1 MTR_8g027780 33.967 368 215 9 148 495 102 461 4.49e-49 179
MS.gene33874.t1 MTR_4g059460 28.537 410 213 9 165 501 63 465 5.15e-49 180
MS.gene33874.t1 MTR_1g102410 32.821 390 222 10 134 497 34 409 2.38e-48 179
MS.gene33874.t1 MTR_8g027775 32.383 386 228 11 135 495 80 457 2.82e-47 174
MS.gene33874.t1 MTR_4g010565 29.167 384 241 10 148 516 125 492 5.01e-45 167
MS.gene33874.t1 MTR_3g107420 29.248 359 237 7 148 502 48 393 4.70e-44 162
MS.gene33874.t1 MTR_1g012420 29.248 359 237 7 148 502 48 393 1.42e-43 161
MS.gene33874.t1 MTR_2g063080 27.446 419 247 7 139 505 101 514 3.21e-43 163
MS.gene33874.t1 MTR_6g039840 28.906 384 233 9 144 503 107 474 1.21e-42 163
MS.gene33874.t1 MTR_8g027770 32.961 358 207 9 158 495 212 556 5.97e-42 161
MS.gene33874.t1 MTR_5g092560 32.184 348 219 8 166 506 95 432 2.17e-41 160
MS.gene33874.t1 MTR_1g061970 30.536 429 236 17 136 523 68 475 5.38e-41 155
MS.gene33874.t1 MTR_8g097000 32.778 360 217 10 158 501 299 649 5.46e-41 159
MS.gene33874.t1 MTR_7g057250 27.902 448 245 12 148 525 164 603 1.23e-39 155
MS.gene33874.t1 MTR_8g027780 32.362 343 204 9 148 470 102 436 2.74e-38 147
MS.gene33874.t1 MTR_4g068990 30.831 373 237 11 143 507 73 432 1.40e-37 149
MS.gene33874.t1 MTR_1g083130 27.053 414 237 9 146 508 25 424 7.48e-36 142
MS.gene33874.t1 MTR_4g112320 34.454 238 145 6 261 490 21 255 8.81e-35 136
MS.gene33874.t1 MTR_3g118120 26.943 386 245 11 144 501 129 505 3.35e-34 137
MS.gene33874.t1 MTR_4g049910 29.474 380 215 10 148 501 56 408 5.89e-33 132
MS.gene33874.t1 MTR_2g043360 27.019 322 162 6 259 507 29 350 6.64e-33 132
MS.gene33874.t1 MTR_0915s0010 29.352 293 189 5 164 454 6 282 5.29e-32 125
MS.gene33874.t1 MTR_4g131750 27.877 391 221 12 139 490 74 442 5.59e-29 120
MS.gene33874.t1 MTR_6g039840 29.276 304 176 8 223 503 11 298 7.08e-29 120
MS.gene33874.t1 MTR_1g046570 28.881 277 183 8 266 532 126 398 9.99e-29 119
MS.gene33874.t1 MTR_5g069330 25.275 364 250 10 145 502 5 352 1.12e-28 119
MS.gene33874.t1 MTR_5g069330 25.275 364 250 10 145 502 1 348 1.30e-28 119
MS.gene33874.t1 MTR_4g459350 31.527 203 113 4 165 348 60 255 1.71e-25 106
MS.gene33874.t1 MTR_8g018370 24.377 361 229 7 177 532 3 324 7.80e-25 105
MS.gene33874.t1 MTR_4g077370 35.238 210 109 7 147 348 250 440 3.57e-24 106
MS.gene33874.t1 MTR_4g077370 32.227 211 99 6 147 348 2 177 1.17e-18 89.4
MS.gene33874.t1 MTR_5g092560 31.401 207 136 4 306 506 15 221 1.19e-23 105
MS.gene33874.t1 MTR_8g042690 25.429 350 222 10 170 501 41 369 5.60e-22 99.8
MS.gene33874.t1 MTR_4g068990 30.769 208 137 5 306 507 15 221 2.79e-21 98.2
MS.gene33874.t1 MTR_5g077690 25.916 382 216 12 184 505 70 444 3.14e-20 94.7
MS.gene33874.t1 MTR_3g107420 28.571 203 130 5 148 348 48 237 9.84e-20 90.9
MS.gene33874.t1 MTR_1g022305 23.947 451 235 17 145 590 1 348 2.39e-19 91.7
MS.gene33874.t1 MTR_8g032230 34.756 164 93 4 145 307 8 158 3.75e-19 85.5
MS.gene33874.t1 MTR_3g063040 24.507 355 230 11 155 498 2 329 2.07e-13 73.9
MS.gene33874.t1 MTR_8g062720 25.248 202 133 4 322 507 345 544 1.24e-11 67.8
MS.gene33874.t1 MTR_8g080160 31.356 118 76 1 390 502 480 597 3.31e-11 67.0
MS.gene33874.t1 MTR_0427s0010 27.358 212 117 6 316 510 1 192 7.99e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33874.t1 AT5G51280 82.423 586 102 1 6 591 7 591 0.0 1003
MS.gene33874.t1 AT4G33370 80.702 513 99 0 79 591 30 542 0.0 871
MS.gene33874.t1 AT1G55150 38.795 415 241 5 120 531 73 477 5.49e-98 308
MS.gene33874.t1 AT1G55150 38.795 415 241 5 120 531 98 502 3.14e-97 306
MS.gene33874.t1 AT5G63120 40.714 420 234 7 117 532 136 544 4.47e-96 305
MS.gene33874.t1 AT2G47330 40.798 451 250 8 87 531 168 607 2.87e-93 303
MS.gene33874.t1 AT3G58510 38.025 405 232 6 136 528 141 538 1.69e-91 294
MS.gene33874.t1 AT3G58510 38.025 405 232 6 136 528 141 538 1.69e-91 294
MS.gene33874.t1 AT3G58510 38.025 405 232 6 136 528 141 538 1.69e-91 294
MS.gene33874.t1 AT3G58570 38.386 409 231 7 134 528 134 535 5.18e-91 294
MS.gene33874.t1 AT2G42520 37.288 413 230 8 134 528 147 548 1.16e-89 290
MS.gene33874.t1 AT2G42520 37.288 413 230 8 134 528 147 548 1.16e-89 290
MS.gene33874.t1 AT2G42520 37.288 413 230 8 134 528 147 548 1.16e-89 290
MS.gene33874.t1 AT1G20920 37.441 422 249 5 117 533 162 573 1.54e-87 289
MS.gene33874.t1 AT2G33730 33.647 532 294 12 39 529 194 707 1.61e-87 287
MS.gene33874.t1 AT1G20920 37.441 422 249 5 117 533 500 911 7.63e-86 290
MS.gene33874.t1 AT1G20920 37.441 422 249 5 117 533 500 911 7.63e-86 290
MS.gene33874.t1 AT1G20920 37.441 422 249 5 117 533 500 911 7.63e-86 290
MS.gene33874.t1 AT1G20920 37.441 422 249 5 117 533 500 911 7.63e-86 290
MS.gene33874.t1 AT1G20920 37.441 422 249 5 117 533 500 911 7.63e-86 290
MS.gene33874.t1 AT1G20920 37.441 422 249 5 117 533 500 911 7.63e-86 290
MS.gene33874.t1 AT3G01540 38.499 413 232 8 126 531 138 535 1.72e-84 276
MS.gene33874.t1 AT3G01540 38.499 413 232 8 126 531 138 535 1.88e-84 276
MS.gene33874.t1 AT3G01540 38.499 413 232 8 126 531 138 535 1.88e-84 276
MS.gene33874.t1 AT3G01540 38.499 413 232 8 126 531 138 535 1.88e-84 276
MS.gene33874.t1 AT5G14610 38.142 409 231 8 130 531 145 538 4.25e-83 273
MS.gene33874.t1 AT5G14610 38.142 409 231 8 130 531 145 538 4.86e-83 273
MS.gene33874.t1 AT5G14610 38.142 409 231 8 130 531 153 546 6.39e-83 273
MS.gene33874.t1 AT1G31970 37.981 416 222 12 129 525 95 493 1.21e-79 261
MS.gene33874.t1 AT3G06480 39.078 412 233 7 125 531 414 812 4.76e-79 270
MS.gene33874.t1 AT5G14610 38.624 378 212 7 160 531 44 407 3.47e-77 254
MS.gene33874.t1 AT5G14610 38.624 378 212 7 160 531 44 407 3.47e-77 254
MS.gene33874.t1 AT5G14610 37.590 415 233 10 127 531 208 606 3.52e-77 259
MS.gene33874.t1 AT3G09620 35.731 417 218 7 117 525 368 742 4.47e-73 253
MS.gene33874.t1 AT3G09620 35.731 417 218 7 117 525 367 741 4.79e-73 252
MS.gene33874.t1 AT5G63120 39.828 349 196 6 117 461 136 474 9.91e-73 241
MS.gene33874.t1 AT5G63120 39.828 349 196 6 117 461 136 474 1.37e-72 241
MS.gene33874.t1 AT5G63120 39.828 349 196 6 117 461 136 474 1.37e-72 241
MS.gene33874.t1 AT5G63120 39.828 349 196 6 117 461 136 474 1.37e-72 241
MS.gene33874.t1 AT1G28180 37.569 362 203 7 176 529 3 349 3.13e-67 223
MS.gene33874.t1 AT5G60990 33.604 369 233 4 145 507 8 370 3.29e-65 221
MS.gene33874.t1 AT1G77030 34.010 394 242 6 148 537 30 409 8.02e-64 225
MS.gene33874.t1 AT5G14610 35.196 358 213 7 130 481 153 497 1.45e-62 214
MS.gene33874.t1 AT1G16280 34.063 411 239 10 147 548 59 446 2.38e-62 214
MS.gene33874.t1 AT3G09720 34.783 414 230 13 101 500 96 483 2.67e-61 212
MS.gene33874.t1 AT3G09720 34.783 414 230 13 101 500 96 483 2.67e-61 212
MS.gene33874.t1 AT3G06480 37.429 350 202 6 126 470 415 752 2.51e-60 214
MS.gene33874.t1 AT3G22330 34.383 381 228 9 157 530 115 480 2.71e-60 211
MS.gene33874.t1 AT3G02065 30.456 417 276 7 121 531 83 491 3.63e-60 208
MS.gene33874.t1 AT3G02065 30.456 417 276 7 121 531 83 491 3.63e-60 208
MS.gene33874.t1 AT4G16630 32.840 405 243 7 147 537 168 557 1.09e-59 213
MS.gene33874.t1 AT5G26742 33.170 407 229 8 151 537 107 490 3.04e-59 211
MS.gene33874.t1 AT5G26742 33.170 407 229 8 151 537 107 490 3.08e-59 211
MS.gene33874.t1 AT3G22310 34.986 363 221 7 150 508 120 471 1.95e-58 206
MS.gene33874.t1 AT3G22310 34.986 363 221 7 150 508 120 471 2.54e-58 206
MS.gene33874.t1 AT3G13920 29.904 418 271 9 118 532 14 412 3.58e-58 201
MS.gene33874.t1 AT3G13920 31.026 390 250 8 146 532 29 402 7.54e-58 199
MS.gene33874.t1 AT5G26742 33.687 377 207 8 181 537 44 397 1.27e-56 202
MS.gene33874.t1 AT3G13920 29.904 418 266 10 118 532 14 407 1.33e-56 196
MS.gene33874.t1 AT1G51380 30.691 391 249 6 145 532 21 392 3.51e-56 194
MS.gene33874.t1 AT3G13920 29.540 413 270 8 118 527 14 408 9.34e-56 194
MS.gene33874.t1 AT3G13920 29.540 413 270 8 118 527 18 412 9.57e-56 194
MS.gene33874.t1 AT1G72730 30.334 389 252 8 147 532 42 414 1.50e-55 193
MS.gene33874.t1 AT5G65900 32.861 353 216 7 146 490 154 493 3.70e-55 197
MS.gene33874.t1 AT1G54270 31.122 392 247 8 146 532 39 412 2.00e-54 190
MS.gene33874.t1 AT3G18600 32.597 362 229 6 133 490 77 427 2.97e-54 194
MS.gene33874.t1 AT3G19760 30.789 393 249 7 145 532 34 408 4.01e-54 189
MS.gene33874.t1 AT1G71370 32.105 380 236 9 141 507 9 379 1.10e-53 192
MS.gene33874.t1 AT1G54270 31.818 374 232 8 164 532 52 407 3.43e-53 187
MS.gene33874.t1 AT3G02065 30.110 362 241 6 174 531 1 354 4.24e-51 180
MS.gene33874.t1 AT5G62190 31.854 383 237 9 140 512 93 461 5.45e-51 187
MS.gene33874.t1 AT5G05450 31.510 384 225 10 146 501 9 382 2.46e-50 183
MS.gene33874.t1 AT3G61240 30.618 356 229 6 148 501 126 465 7.65e-49 177
MS.gene33874.t1 AT3G61240 30.618 356 229 6 148 501 126 465 7.65e-49 177
MS.gene33874.t1 AT4G00660 29.293 396 262 6 139 532 123 502 9.63e-49 177
MS.gene33874.t1 AT4G00660 29.293 396 262 6 139 532 123 502 9.63e-49 177
MS.gene33874.t1 AT2G45810 30.601 366 237 5 139 502 146 496 1.56e-48 177
MS.gene33874.t1 AT2G40700 28.986 414 210 9 165 502 50 455 5.50e-47 174
MS.gene33874.t1 AT1G12770 27.470 415 241 8 144 505 108 515 8.30e-46 170
MS.gene33874.t1 AT1G12770 27.470 415 241 8 144 505 108 515 8.30e-46 170
MS.gene33874.t1 AT5G11170 29.474 380 236 11 136 501 31 392 4.69e-45 165
MS.gene33874.t1 AT5G11200 29.474 380 236 11 136 501 31 392 4.69e-45 165
MS.gene33874.t1 AT5G54910 32.249 369 226 10 144 502 69 423 7.61e-44 167
MS.gene33874.t1 AT5G63630 31.818 352 219 7 158 495 66 410 3.01e-43 162
MS.gene33874.t1 AT4G09730 28.906 384 231 10 145 503 111 477 2.56e-42 161
MS.gene33874.t1 AT5G63630 31.818 352 219 7 158 495 260 604 2.78e-42 162
MS.gene33874.t1 AT2G07750 30.673 401 252 9 113 495 340 732 1.13e-40 158
MS.gene33874.t1 AT5G08610 31.461 356 215 7 158 495 394 738 1.30e-40 158
MS.gene33874.t1 AT5G11200 27.725 422 230 13 136 501 31 433 6.59e-40 152
MS.gene33874.t1 AT5G11170 30.675 326 199 10 185 501 2 309 7.21e-40 149
MS.gene33874.t1 AT1G63250 30.137 365 237 7 144 495 326 685 2.19e-39 154
MS.gene33874.t1 AT5G08620 30.899 356 217 8 158 495 92 436 4.90e-39 151
MS.gene33874.t1 AT5G11200 26.941 438 231 13 136 501 31 451 1.90e-38 148
MS.gene33874.t1 AT3G53110 29.381 388 225 13 135 495 80 445 6.12e-38 147
MS.gene33874.t1 AT1G71280 29.940 334 193 10 139 465 11 310 8.69e-36 137
MS.gene33874.t1 AT1G71280 29.586 338 197 10 139 469 11 314 2.46e-35 139
MS.gene33874.t1 AT3G06980 28.982 383 236 13 146 501 374 747 2.65e-33 136
MS.gene33874.t1 AT3G16840 27.209 430 232 11 148 506 192 611 1.13e-32 134
MS.gene33874.t1 AT3G16840 27.209 430 232 11 148 506 193 612 1.13e-32 134
MS.gene33874.t1 AT4G34910 27.569 399 226 11 144 491 44 430 6.62e-31 128
MS.gene33874.t1 AT5G19210 26.579 380 243 10 136 490 66 434 1.13e-28 120
MS.gene33874.t1 AT1G51380 28.782 271 175 5 145 412 21 276 2.00e-27 112
MS.gene33874.t1 AT1G51380 30.698 215 135 3 145 359 21 221 5.18e-25 105
MS.gene33874.t1 AT1G51380 30.698 215 135 3 145 359 21 221 6.81e-25 105
MS.gene33874.t1 AT5G19210 26.609 233 147 7 281 490 46 277 2.80e-17 83.6
MS.gene33874.t1 AT5G19210 26.609 233 147 7 281 490 46 277 2.80e-17 83.6
MS.gene33874.t1 AT1G28180 37.008 127 64 3 176 297 3 118 1.14e-14 71.2
MS.gene33874.t1 AT1G27880 25.068 367 214 13 173 515 274 603 1.24e-13 74.7
MS.gene33874.t1 AT1G27880 25.068 367 214 13 173 515 274 603 1.35e-13 74.7

Find 146 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATTCTGATGTTCTAATTT+TGG 0.122328 1.2:-12899193 None:intergenic
CCATCCAATGGTAGCTAAAA+TGG 0.220873 1.2:+12897497 None:intergenic
GGAATTGCTTTCACGGGTTC+TGG 0.240475 1.2:+12898065 MS.gene33874:CDS
CACGTAGTTGTTGCCACGCC+TGG 0.267897 1.2:+12898341 MS.gene33874:CDS
CGTCCCACATTGACAATAAT+AGG 0.278247 1.2:-12898575 None:intergenic
TTGTTACCTTTGAAGGAATC+TGG 0.284937 1.2:+12898239 MS.gene33874:CDS
CTCAGACCTTTGCTTTGTAT+TGG 0.297978 1.2:+12898272 MS.gene33874:CDS
AATAATAGGAAGGATTCTTT+TGG 0.300703 1.2:+12899229 MS.gene33874:CDS
TTTCTAGAATCATCATCTAT+TGG 0.306294 1.2:-12897612 None:intergenic
ACAATTCTTGTTACCTTTGA+AGG 0.309882 1.2:+12898232 MS.gene33874:CDS
GGCGAATCTTGATGTTATTC+AGG 0.315986 1.2:+12898607 MS.gene33874:CDS
TCAATCAGCTCAGCCAAAAC+TGG 0.319761 1.2:-12899079 None:intergenic
GAATGGAAAATGCAAATAAT+AGG 0.322051 1.2:+12899215 MS.gene33874:CDS
TGTGCAGCCAACACCTATTC+AGG 0.343871 1.2:+12898001 MS.gene33874:CDS
CAAGCAATGTTGTCTCAGTT+TGG 0.345951 1.2:-12899015 None:intergenic
TGTGGTAATTGTGGCGGGCT+TGG 0.359741 1.2:+12899151 MS.gene33874:CDS
TTAATGACATGTTGAATATC+AGG 0.372122 1.2:-12898893 None:intergenic
GGACCTTCACCGGGAACTAT+AGG 0.383718 1.2:-12898149 None:intergenic
AGCCTCCTTTGCATATTAGA+AGG 0.387546 1.2:+12897838 MS.gene33874:CDS
AGACCTTTGCTTTGTATTGG+TGG 0.394935 1.2:+12898275 MS.gene33874:CDS
TCCCTCCCAGATAAAATAAC+AGG 0.398029 1.2:-12898035 None:intergenic
TACAGAACAGATTGTTCAAC+AGG 0.398392 1.2:+12897713 MS.gene33874:CDS
GTGGAAGCCGTGGCAATTCA+TGG 0.398757 1.2:+12898767 MS.gene33874:CDS
ACTGGAAGATGTGGCAAAAC+TGG 0.401990 1.2:+12898965 MS.gene33874:CDS
CTAATTCACGAACCGACATA+AGG 0.404039 1.2:-12897773 None:intergenic
GGAAGCCCTTGCACCTGAAT+AGG 0.407757 1.2:-12898014 None:intergenic
ATCAGGAAAATCCAAGCCTT+TGG 0.408303 1.2:-12898876 None:intergenic
CACGGGTTCTGGCAAAACTT+TGG 0.414563 1.2:+12898076 MS.gene33874:CDS
GAAAATTATGTCCATAGGAT+TGG 0.415181 1.2:+12898938 MS.gene33874:CDS
TATTTGTCCATCGAGGGAAT+TGG 0.416876 1.2:+12898184 MS.gene33874:CDS
CACTGGAATAAGTGGTATCA+AGG 0.423443 1.2:+12899126 MS.gene33874:CDS
ATTGTCAATGTGGGACGTGC+AGG 0.426137 1.2:+12898581 MS.gene33874:CDS
TGCATTGCCATCATGATCAT+TGG 0.427490 1.2:-12898110 None:intergenic
TAATTCACGAACCGACATAA+GGG 0.432495 1.2:-12897772 None:intergenic
TTCTCACAAAATATCAAAAC+AGG 0.435087 1.2:-12898698 None:intergenic
GTCATTTAAGACTGGAAAGA+AGG 0.439351 1.2:+12898826 MS.gene33874:CDS
TAAACCCAGTCTACTTGTTA+AGG 0.442729 1.2:+12897653 MS.gene33874:CDS
AACATCTTCAAAATAGGATC+CGG 0.446469 1.2:-12897963 None:intergenic
AATGGAAGAAGATGATTATG+AGG 0.446862 1.2:+12897515 MS.gene33874:CDS
TATAACGAACCATTGCCTAC+TGG 0.451678 1.2:+12897810 MS.gene33874:CDS
CTTATATCATCTTCAAATCC+AGG 0.453322 1.2:-12898452 None:intergenic
TTCCAGTACGTCCAATCCTA+TGG 0.454678 1.2:-12898949 None:intergenic
CAAAATATCAAAACAGGAGG+TGG 0.462408 1.2:-12898692 None:intergenic
GCTATTTCGTCATTTAAGAC+TGG 0.467758 1.2:+12898818 MS.gene33874:CDS
GGACAAATAATCAGACCAAA+TGG 0.468369 1.2:-12898170 None:intergenic
GTCCATAGGATTGGACGTAC+TGG 0.469423 1.2:+12898947 MS.gene33874:CDS
TGATATTGCATCCAAAGGCT+TGG 0.472039 1.2:+12898865 MS.gene33874:CDS
AATGATCATGATGGCAATGC+AGG 0.472381 1.2:+12898112 MS.gene33874:CDS
ATTACGATGCCTATAGTTCC+CGG 0.472488 1.2:+12898140 MS.gene33874:CDS
ATAACGAACCATTGCCTACT+GGG 0.472936 1.2:+12897811 MS.gene33874:CDS
CATGAGTATCTTCTCTTGAA+AGG 0.476814 1.2:+12898743 MS.gene33874:CDS
TTATCTGGGAGGGATATGAT+TGG 0.476899 1.2:+12898044 MS.gene33874:CDS
ATTTCTTCATCATCTTCCAT+TGG 0.477637 1.2:-12899103 None:intergenic
ATGGCGCGACGCTTCGCCAC+AGG 0.477803 1.2:-12897546 None:intergenic
TATGATTGGAATTGCTTTCA+CGG 0.478341 1.2:+12898058 MS.gene33874:CDS
GCTGATCGATTGGTAGATCC+TGG 0.479567 1.2:+12898434 MS.gene33874:CDS
TTTGAAGGAATCTGGATATC+CGG 0.481624 1.2:+12898247 MS.gene33874:CDS
CAGATAATTTCCCTTTGCGT+TGG 0.485668 1.2:-12897587 None:intergenic
TTGAAGATGTTGAAAACCAA+GGG 0.486333 1.2:+12897975 MS.gene33874:CDS
TGTTGGCTGCACAATCCCCT+TGG 0.486336 1.2:-12897991 None:intergenic
CAGACCAAATGGACCTTCAC+CGG 0.492977 1.2:-12898159 None:intergenic
GTTCCCGGTGAAGGTCCATT+TGG 0.493227 1.2:+12898155 MS.gene33874:CDS
TCCTTAAAATTCTTAATCGC+GGG 0.495673 1.2:-12897930 None:intergenic
AAATGAATCTTGATAACTGC+AGG 0.497521 1.2:+12898393 MS.gene33874:CDS
GAAGAAATCACTGGAATAAG+TGG 0.497632 1.2:+12899118 MS.gene33874:CDS
ACGTAGTTGTTGCCACGCCT+GGG 0.501414 1.2:+12898342 MS.gene33874:CDS
ATTAGAACATCAGAATAGAA+TGG 0.501977 1.2:+12899198 MS.gene33874:CDS
CAGTGGCATATAATTGTCAA+TGG 0.504194 1.2:+12897894 MS.gene33874:CDS
TGTGGCGAAGCGTCGCGCCA+TGG 0.508155 1.2:+12897548 MS.gene33874:CDS
CAATCAGCTCAGCCAAAACT+GGG 0.508157 1.2:-12899078 None:intergenic
TGAGGCCTTAACAAGTAGAC+TGG 0.510721 1.2:-12897658 None:intergenic
ATCCTTAAAATTCTTAATCG+CGG 0.511417 1.2:-12897931 None:intergenic
ACAGCTTGAGATTGTCAAGA+AGG 0.518951 1.2:+12898313 MS.gene33874:CDS
ATGATTGGAATTGCTTTCAC+GGG 0.521318 1.2:+12898059 MS.gene33874:CDS
GAAGATGATGAAGAAATCAC+TGG 0.522383 1.2:+12899109 MS.gene33874:CDS
CAAAGCAAAGGTCTGAGCTC+CGG 0.523344 1.2:-12898266 None:intergenic
AATTCATGGAGACAAGGATC+AGG 0.523834 1.2:+12898781 MS.gene33874:CDS
GAGAATCTTCTGAGCTTCCA+TGG 0.525892 1.2:-12897565 None:intergenic
TTTGAAGATGTTGAAAACCA+AGG 0.526623 1.2:+12897974 MS.gene33874:CDS
CAGCTTGAGATTGTCAAGAA+GGG 0.528031 1.2:+12898314 MS.gene33874:CDS
AAAATATCAAAACAGGAGGT+GGG 0.530476 1.2:-12898691 None:intergenic
GACTGGAAAGAAGGATGTGT+TGG 0.532068 1.2:+12898835 MS.gene33874:CDS
TTAAAATTCTTAATCGCGGG+TGG 0.532306 1.2:-12897927 None:intergenic
GTTAGATGAGGCTGATCGAT+TGG 0.534300 1.2:+12898424 MS.gene33874:CDS
TTGTCAATGTGGGACGTGCA+GGG 0.535671 1.2:+12898582 MS.gene33874:CDS
CAATGTGGGACGTGCAGGGG+CGG 0.535678 1.2:+12898586 MS.gene33874:CDS
AAATTGAAAATTATGTCCAT+AGG 0.540038 1.2:+12898933 MS.gene33874:CDS
AGAAGATTCTCCAACGCAAA+GGG 0.540048 1.2:+12897577 MS.gene33874:CDS
TGCCACGCCTGGGAGGTTGA+AGG 0.543540 1.2:+12898352 MS.gene33874:CDS
GGCTGAGCTGATTGATCCAA+TGG 0.544866 1.2:+12899087 MS.gene33874:CDS
AACATATCCTTCAACCTCCC+AGG 0.545718 1.2:-12898359 None:intergenic
GGAAAATGCAAATAATAGGA+AGG 0.546682 1.2:+12899219 MS.gene33874:CDS
TGTTCTGTAACACTAATCTC+AGG 0.547023 1.2:-12897699 None:intergenic
GTCATCCTTCTAATATGCAA+AGG 0.548607 1.2:-12897843 None:intergenic
AGGGATGTGGTAATTGTGGC+GGG 0.551578 1.2:+12899146 MS.gene33874:CDS
TCTGATTATTTGTCCATCGA+GGG 0.551896 1.2:+12898178 MS.gene33874:CDS
TGCCTCGCCAATTCCCTCGA+TGG 0.556276 1.2:-12898191 None:intergenic
CGGTTCGTGAATTAGCGAAA+GGG 0.557381 1.2:+12897781 MS.gene33874:CDS
GGATATCCAGATTCCTTCAA+AGG 0.559706 1.2:-12898245 None:intergenic
ATAAGTGGTATCAAGGGATG+TGG 0.562597 1.2:+12899133 MS.gene33874:CDS
CTTGTCTCCATGAATTGCCA+CGG 0.563651 1.2:-12898774 None:intergenic
ATCAAGGGATGTGGTAATTG+TGG 0.563728 1.2:+12899142 MS.gene33874:CDS
GGCTTCCACCCAGTAGGCAA+TGG 0.565220 1.2:-12897819 None:intergenic
TTTGGATCTGGAGGTTACAA+AGG 0.569518 1.2:+12899247 MS.gene33874:CDS
GCAACTGATATTGCATCCAA+AGG 0.573465 1.2:+12898860 MS.gene33874:CDS
AAAACCTATTATTGTCAATG+TGG 0.574587 1.2:+12898571 MS.gene33874:CDS
AGACCAAATGGACCTTCACC+GGG 0.576562 1.2:-12898158 None:intergenic
AGCTTGAGATTGTCAAGAAG+GGG 0.576627 1.2:+12898315 MS.gene33874:CDS
TGGGAGGTTGAAGGATATGT+TGG 0.576648 1.2:+12898361 MS.gene33874:CDS
CAGAAGATTCTCCAACGCAA+AGG 0.577959 1.2:+12897576 MS.gene33874:CDS
GTCTGATTATTTGTCCATCG+AGG 0.580654 1.2:+12898177 MS.gene33874:CDS
TGTGCTTCCAATGATCATGA+TGG 0.581752 1.2:+12898103 MS.gene33874:CDS
AAATATCAAAACAGGAGGTG+GGG 0.587549 1.2:-12898690 None:intergenic
TTGTTCTTTCTTTAACTGTG+AGG 0.588178 1.2:-12897676 None:intergenic
TTGATCACTTCAAAGCTCAA+AGG 0.591719 1.2:+12898483 MS.gene33874:CDS
TGTCAATGTGGGACGTGCAG+GGG 0.592163 1.2:+12898583 MS.gene33874:CDS
AAACCTATTATTGTCAATGT+GGG 0.592275 1.2:+12898572 MS.gene33874:CDS
CCAACACCTATTCAGGTGCA+AGG 0.595863 1.2:+12898008 MS.gene33874:CDS
AAGGGATGTGGTAATTGTGG+CGG 0.596593 1.2:+12899145 MS.gene33874:CDS
ATGCCTATAGTTCCCGGTGA+AGG 0.596628 1.2:+12898146 MS.gene33874:CDS
GTCCATCGAGGGAATTGGCG+AGG 0.598686 1.2:+12898189 MS.gene33874:CDS
CAGGTATTTAACGTTAGATG+AGG 0.599810 1.2:+12898412 MS.gene33874:CDS
TCGGTTCGTGAATTAGCGAA+AGG 0.600474 1.2:+12897780 MS.gene33874:CDS
ACTGGAATAAGTGGTATCAA+GGG 0.600486 1.2:+12899127 MS.gene33874:CDS
CGTGGCAATTCATGGAGACA+AGG 0.604702 1.2:+12898775 MS.gene33874:CDS
ATCCTTCAACCTCCCAGGCG+TGG 0.609061 1.2:-12898354 None:intergenic
CCTTGCACCTGAATAGGTGT+TGG 0.609165 1.2:-12898008 None:intergenic
ATCCTTCTAATATGCAAAGG+AGG 0.612632 1.2:-12897840 None:intergenic
GTATCTTCTCTTGAAAGGAG+TGG 0.613245 1.2:+12898748 MS.gene33874:CDS
AGTAGAGTATGTCAAACAAG+AGG 0.613269 1.2:+12898631 MS.gene33874:CDS
TAGTTGTTGCCACGCCTGGG+AGG 0.621410 1.2:+12898345 MS.gene33874:CDS
CAACACCTATTCAGGTGCAA+GGG 0.626640 1.2:+12898009 MS.gene33874:CDS
GAGGCCTTAACAAGTAGACT+GGG 0.630843 1.2:-12897657 None:intergenic
ATTCCACCAATACAAAGCAA+AGG 0.634010 1.2:-12898278 None:intergenic
ATTGGACGTACTGGAAGATG+TGG 0.635262 1.2:+12898956 MS.gene33874:CDS
TCACAAAATATCAAAACAGG+AGG 0.646136 1.2:-12898695 None:intergenic
ATCAGCTCAGCCAAAACTGG+GGG 0.648559 1.2:-12899076 None:intergenic
GATCATGATGGCAATGCAGG+AGG 0.660735 1.2:+12898115 MS.gene33874:CDS
CTTGAAAGGAGTGGAAGCCG+TGG 0.660852 1.2:+12898757 MS.gene33874:CDS
AATCAGCTCAGCCAAAACTG+GGG 0.665692 1.2:-12899077 None:intergenic
TGAAGATGTTGAAAACCAAG+GGG 0.665813 1.2:+12897976 MS.gene33874:CDS
GCTTGAGATTGTCAAGAAGG+GGG 0.666059 1.2:+12898316 MS.gene33874:CDS
AAAGGAGGCTTCCACCCAGT+AGG 0.671260 1.2:-12897825 None:intergenic
ACGAACCATTGCCTACTGGG+TGG 0.698489 1.2:+12897814 MS.gene33874:CDS
TTATGAGGAGTATATTCCTG+TGG 0.702818 1.2:+12897530 MS.gene33874:CDS
TGATTCTAGAAAACTAAGTG+TGG 0.748043 1.2:+12897623 MS.gene33874:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTGAAAATTATGTCCAT+AGG + chr1.2:12898933-12898952 MS.gene33874:CDS 20.0%
!!! TCTAGCAAAATTTTGAATTT+TGG - chr1.2:12898540-12898559 None:intergenic 20.0%
!!! TTAAGAATTTTAAGGATATG+AGG + chr1.2:12897937-12897956 MS.gene33874:CDS 20.0%
!!! TTTGATATTTTGTGAGAATA+AGG + chr1.2:12898703-12898722 MS.gene33874:CDS 20.0%
! AAAACCTATTATTGTCAATG+TGG + chr1.2:12898571-12898590 MS.gene33874:CDS 25.0%
! AAACCTATTATTGTCAATGT+GGG + chr1.2:12898572-12898591 MS.gene33874:CDS 25.0%
! ATCCTTAAAATTCTTAATCG+CGG - chr1.2:12897934-12897953 None:intergenic 25.0%
! ATTAGAACATCAGAATAGAA+TGG + chr1.2:12899198-12899217 MS.gene33874:CDS 25.0%
! GAATGGAAAATGCAAATAAT+AGG + chr1.2:12899215-12899234 MS.gene33874:CDS 25.0%
! TGATAAAAAAACCCTTATGT+CGG + chr1.2:12897761-12897780 MS.gene33874:CDS 25.0%
! TTAATGACATGTTGAATATC+AGG - chr1.2:12898896-12898915 None:intergenic 25.0%
! TTCTCACAAAATATCAAAAC+AGG - chr1.2:12898701-12898720 None:intergenic 25.0%
!! AATAATAGGAAGGATTCTTT+TGG + chr1.2:12899229-12899248 MS.gene33874:CDS 25.0%
!! ACATAATTTTCAATTTCAGC+AGG - chr1.2:12898929-12898948 None:intergenic 25.0%
!! ATCAAGATTCATTTTCTTCT+TGG - chr1.2:12898387-12898406 None:intergenic 25.0%
!! CTATTCTGATGTTCTAATTT+TGG - chr1.2:12899196-12899215 None:intergenic 25.0%
!! GTTTTCAACATCTTCAAAAT+AGG - chr1.2:12897972-12897991 None:intergenic 25.0%
!!! GCAAAATTTTGAATTTTGGT+TGG - chr1.2:12898536-12898555 None:intergenic 25.0%
!!! TTTCTAGAATCATCATCTAT+TGG - chr1.2:12897615-12897634 None:intergenic 25.0%
AAAATATCAAAACAGGAGGT+GGG - chr1.2:12898694-12898713 None:intergenic 30.0%
AACATCTTCAAAATAGGATC+CGG - chr1.2:12897966-12897985 None:intergenic 30.0%
AATGGAAGAAGATGATTATG+AGG + chr1.2:12897515-12897534 MS.gene33874:CDS 30.0%
ACAATTCTTGTTACCTTTGA+AGG + chr1.2:12898232-12898251 MS.gene33874:CDS 30.0%
ATTTCTTCATCATCTTCCAT+TGG - chr1.2:12899106-12899125 None:intergenic 30.0%
CTTATATCATCTTCAAATCC+AGG - chr1.2:12898455-12898474 None:intergenic 30.0%
GAAAATTATGTCCATAGGAT+TGG + chr1.2:12898938-12898957 MS.gene33874:CDS 30.0%
GGAAAATGCAAATAATAGGA+AGG + chr1.2:12899219-12899238 MS.gene33874:CDS 30.0%
GTGATTTGATTCAAAAACAG+TGG + chr1.2:12897877-12897896 MS.gene33874:CDS 30.0%
TATGATTGGAATTGCTTTCA+CGG + chr1.2:12898058-12898077 MS.gene33874:CDS 30.0%
TCACAAAATATCAAAACAGG+AGG - chr1.2:12898698-12898717 None:intergenic 30.0%
TCCTTAAAATTCTTAATCGC+GGG - chr1.2:12897933-12897952 None:intergenic 30.0%
TTGAAGATGTTGAAAACCAA+GGG + chr1.2:12897975-12897994 MS.gene33874:CDS 30.0%
TTGTTCTTTCTTTAACTGTG+AGG - chr1.2:12897679-12897698 None:intergenic 30.0%
TTTGAAGATGTTGAAAACCA+AGG + chr1.2:12897974-12897993 MS.gene33874:CDS 30.0%
! AAATGAATCTTGATAACTGC+AGG + chr1.2:12898393-12898412 MS.gene33874:CDS 30.0%
! GTTCTAATTTTGGACAATCA+AGG - chr1.2:12899186-12899205 None:intergenic 30.0%
! TGATTCTAGAAAACTAAGTG+TGG + chr1.2:12897623-12897642 MS.gene33874:CDS 30.0%
!! TTTTAAGGATATGAGGTTTC+CGG + chr1.2:12897944-12897963 MS.gene33874:CDS 30.0%
!!! AAATTTTGCTAGAAGTGCTT+TGG + chr1.2:12898547-12898566 MS.gene33874:CDS 30.0%
AAATATCAAAACAGGAGGTG+GGG - chr1.2:12898693-12898712 None:intergenic 35.0%
ACTGGAATAAGTGGTATCAA+GGG + chr1.2:12899127-12899146 MS.gene33874:CDS 35.0%
AGTAGAGTATGTCAAACAAG+AGG + chr1.2:12898631-12898650 MS.gene33874:CDS 35.0%
ATCCTTCTAATATGCAAAGG+AGG - chr1.2:12897843-12897862 None:intergenic 35.0%
ATGATTGGAATTGCTTTCAC+GGG + chr1.2:12898059-12898078 MS.gene33874:CDS 35.0%
ATTCCACCAATACAAAGCAA+AGG - chr1.2:12898281-12898300 None:intergenic 35.0%
CAAAATATCAAAACAGGAGG+TGG - chr1.2:12898695-12898714 None:intergenic 35.0%
CAGGTATTTAACGTTAGATG+AGG + chr1.2:12898412-12898431 MS.gene33874:CDS 35.0%
CAGTGGCATATAATTGTCAA+TGG + chr1.2:12897894-12897913 MS.gene33874:CDS 35.0%
CATGAGTATCTTCTCTTGAA+AGG + chr1.2:12898743-12898762 MS.gene33874:CDS 35.0%
GAAGAAATCACTGGAATAAG+TGG + chr1.2:12899118-12899137 MS.gene33874:CDS 35.0%
GAAGATGATGAAGAAATCAC+TGG + chr1.2:12899109-12899128 MS.gene33874:CDS 35.0%
GGACAAATAATCAGACCAAA+TGG - chr1.2:12898173-12898192 None:intergenic 35.0%
GTCATCCTTCTAATATGCAA+AGG - chr1.2:12897846-12897865 None:intergenic 35.0%
TAATTCACGAACCGACATAA+GGG - chr1.2:12897775-12897794 None:intergenic 35.0%
TACAGAACAGATTGTTCAAC+AGG + chr1.2:12897713-12897732 MS.gene33874:CDS 35.0%
TCTGATTATTTGTCCATCGA+GGG + chr1.2:12898178-12898197 MS.gene33874:CDS 35.0%
TGAAGATGTTGAAAACCAAG+GGG + chr1.2:12897976-12897995 MS.gene33874:CDS 35.0%
TGTTCTGTAACACTAATCTC+AGG - chr1.2:12897702-12897721 None:intergenic 35.0%
TTAAAATTCTTAATCGCGGG+TGG - chr1.2:12897930-12897949 None:intergenic 35.0%
TTGATCACTTCAAAGCTCAA+AGG + chr1.2:12898483-12898502 MS.gene33874:CDS 35.0%
TTTGAAGGAATCTGGATATC+CGG + chr1.2:12898247-12898266 MS.gene33874:CDS 35.0%
! ACCCGCGATTAAGAATTTTA+AGG + chr1.2:12897929-12897948 MS.gene33874:CDS 35.0%
! GCTATTTCGTCATTTAAGAC+TGG + chr1.2:12898818-12898837 MS.gene33874:CDS 35.0%
! GTCATTTAAGACTGGAAAGA+AGG + chr1.2:12898826-12898845 MS.gene33874:CDS 35.0%
! TAAACCCAGTCTACTTGTTA+AGG + chr1.2:12897653-12897672 MS.gene33874:CDS 35.0%
! TTATGAGGAGTATATTCCTG+TGG + chr1.2:12897530-12897549 MS.gene33874:CDS 35.0%
! TTGTTACCTTTGAAGGAATC+TGG + chr1.2:12898239-12898258 MS.gene33874:CDS 35.0%
!!! GGCTTCCTGTTATTTTATCT+GGG + chr1.2:12898030-12898049 MS.gene33874:CDS 35.0%
!!! TCCTGTTATTTTATCTGGGA+GGG + chr1.2:12898034-12898053 MS.gene33874:CDS 35.0%
!!! TTCCTGTTATTTTATCTGGG+AGG + chr1.2:12898033-12898052 MS.gene33874:CDS 35.0%
AATGATCATGATGGCAATGC+AGG + chr1.2:12898112-12898131 MS.gene33874:CDS 40.0%
AATTCATGGAGACAAGGATC+AGG + chr1.2:12898781-12898800 MS.gene33874:CDS 40.0%
ACAGCTTGAGATTGTCAAGA+AGG + chr1.2:12898313-12898332 MS.gene33874:CDS 40.0%
AGAAGATTCTCCAACGCAAA+GGG + chr1.2:12897577-12897596 MS.gene33874:CDS 40.0%
AGACCTTTGCTTTGTATTGG+TGG + chr1.2:12898275-12898294 MS.gene33874:CDS 40.0%
AGCCTCCTTTGCATATTAGA+AGG + chr1.2:12897838-12897857 MS.gene33874:CDS 40.0%
AGCTTGAGATTGTCAAGAAG+GGG + chr1.2:12898315-12898334 MS.gene33874:CDS 40.0%
ATAACGAACCATTGCCTACT+GGG + chr1.2:12897811-12897830 MS.gene33874:CDS 40.0%
ATAAGTGGTATCAAGGGATG+TGG + chr1.2:12899133-12899152 MS.gene33874:CDS 40.0%
ATCAAGGGATGTGGTAATTG+TGG + chr1.2:12899142-12899161 MS.gene33874:CDS 40.0%
ATCAGGAAAATCCAAGCCTT+TGG - chr1.2:12898879-12898898 None:intergenic 40.0%
ATTACGATGCCTATAGTTCC+CGG + chr1.2:12898140-12898159 MS.gene33874:CDS 40.0%
CAAGCAATGTTGTCTCAGTT+TGG - chr1.2:12899018-12899037 None:intergenic 40.0%
CACTGGAATAAGTGGTATCA+AGG + chr1.2:12899126-12899145 MS.gene33874:CDS 40.0%
CAGATAATTTCCCTTTGCGT+TGG - chr1.2:12897590-12897609 None:intergenic 40.0%
CAGCTTGAGATTGTCAAGAA+GGG + chr1.2:12898314-12898333 MS.gene33874:CDS 40.0%
CGTCCCACATTGACAATAAT+AGG - chr1.2:12898578-12898597 None:intergenic 40.0%
CTAATTCACGAACCGACATA+AGG - chr1.2:12897776-12897795 None:intergenic 40.0%
CTCAGACCTTTGCTTTGTAT+TGG + chr1.2:12898272-12898291 MS.gene33874:CDS 40.0%
GCAACTGATATTGCATCCAA+AGG + chr1.2:12898860-12898879 MS.gene33874:CDS 40.0%
GGATATCCAGATTCCTTCAA+AGG - chr1.2:12898248-12898267 None:intergenic 40.0%
GGCGAATCTTGATGTTATTC+AGG + chr1.2:12898607-12898626 MS.gene33874:CDS 40.0%
GTATCTTCTCTTGAAAGGAG+TGG + chr1.2:12898748-12898767 MS.gene33874:CDS 40.0%
GTCTGATTATTTGTCCATCG+AGG + chr1.2:12898177-12898196 MS.gene33874:CDS 40.0%
TATAACGAACCATTGCCTAC+TGG + chr1.2:12897810-12897829 MS.gene33874:CDS 40.0%
TATTTGTCCATCGAGGGAAT+TGG + chr1.2:12898184-12898203 MS.gene33874:CDS 40.0%
TCCCTCCCAGATAAAATAAC+AGG - chr1.2:12898038-12898057 None:intergenic 40.0%
TGATATTGCATCCAAAGGCT+TGG + chr1.2:12898865-12898884 MS.gene33874:CDS 40.0%
TGCATTGCCATCATGATCAT+TGG - chr1.2:12898113-12898132 None:intergenic 40.0%
TGTGCTTCCAATGATCATGA+TGG + chr1.2:12898103-12898122 MS.gene33874:CDS 40.0%
TTATCTGGGAGGGATATGAT+TGG + chr1.2:12898044-12898063 MS.gene33874:CDS 40.0%
TTTGGATCTGGAGGTTACAA+AGG + chr1.2:12899247-12899266 MS.gene33874:CDS 40.0%
! AAGGATTCTTTTGGATCTGG+AGG + chr1.2:12899238-12899257 MS.gene33874:CDS 40.0%
! AGGAAGGATTCTTTTGGATC+TGG + chr1.2:12899235-12899254 MS.gene33874:CDS 40.0%
! TCTGTTTTGCTTCTCGCAAA+AGG - chr1.2:12899051-12899070 None:intergenic 40.0%
! TTTTGCGAGAAGCAAAACAG+AGG + chr1.2:12899050-12899069 MS.gene33874:CDS 40.0%
!!! GGGCTTCCTGTTATTTTATC+TGG + chr1.2:12898029-12898048 MS.gene33874:CDS 40.0%
AACATATCCTTCAACCTCCC+AGG - chr1.2:12898362-12898381 None:intergenic 45.0%
AAGGGATGTGGTAATTGTGG+CGG + chr1.2:12899145-12899164 MS.gene33874:CDS 45.0%
AATCAGCTCAGCCAAAACTG+GGG - chr1.2:12899080-12899099 None:intergenic 45.0%
ACTGGAAGATGTGGCAAAAC+TGG + chr1.2:12898965-12898984 MS.gene33874:CDS 45.0%
ATTGGACGTACTGGAAGATG+TGG + chr1.2:12898956-12898975 MS.gene33874:CDS 45.0%
CAACACCTATTCAGGTGCAA+GGG + chr1.2:12898009-12898028 MS.gene33874:CDS 45.0%
CAATCAGCTCAGCCAAAACT+GGG - chr1.2:12899081-12899100 None:intergenic 45.0%
CAGAAGATTCTCCAACGCAA+AGG + chr1.2:12897576-12897595 MS.gene33874:CDS 45.0%
CGGTTCGTGAATTAGCGAAA+GGG + chr1.2:12897781-12897800 MS.gene33874:CDS 45.0%
CTTGTCTCCATGAATTGCCA+CGG - chr1.2:12898777-12898796 None:intergenic 45.0%
GACTGGAAAGAAGGATGTGT+TGG + chr1.2:12898835-12898854 MS.gene33874:CDS 45.0%
GAGAATCTTCTGAGCTTCCA+TGG - chr1.2:12897568-12897587 None:intergenic 45.0%
GAGGCCTTAACAAGTAGACT+GGG - chr1.2:12897660-12897679 None:intergenic 45.0%
GCTTGAGATTGTCAAGAAGG+GGG + chr1.2:12898316-12898335 MS.gene33874:CDS 45.0%
GTTAGATGAGGCTGATCGAT+TGG + chr1.2:12898424-12898443 MS.gene33874:CDS 45.0%
TCAATCAGCTCAGCCAAAAC+TGG - chr1.2:12899082-12899101 None:intergenic 45.0%
TGAGGCCTTAACAAGTAGAC+TGG - chr1.2:12897661-12897680 None:intergenic 45.0%
TGGGAGGTTGAAGGATATGT+TGG + chr1.2:12898361-12898380 MS.gene33874:CDS 45.0%
TTCCAGTACGTCCAATCCTA+TGG - chr1.2:12898952-12898971 None:intergenic 45.0%
! TCGGTTCGTGAATTAGCGAA+AGG + chr1.2:12897780-12897799 MS.gene33874:CDS 45.0%
! TTTTCTGTAGGCACTCAAGG+AGG - chr1.2:12898670-12898689 None:intergenic 45.0%
AGACCAAATGGACCTTCACC+GGG - chr1.2:12898161-12898180 None:intergenic 50.0%
AGGGATGTGGTAATTGTGGC+GGG + chr1.2:12899146-12899165 MS.gene33874:CDS 50.0%
ATCAGCTCAGCCAAAACTGG+GGG - chr1.2:12899079-12899098 None:intergenic 50.0%
ATGCCTATAGTTCCCGGTGA+AGG + chr1.2:12898146-12898165 MS.gene33874:CDS 50.0%
ATTGTCAATGTGGGACGTGC+AGG + chr1.2:12898581-12898600 MS.gene33874:CDS 50.0%
CAAAGCAAAGGTCTGAGCTC+CGG - chr1.2:12898269-12898288 None:intergenic 50.0%
CAGACCAAATGGACCTTCAC+CGG - chr1.2:12898162-12898181 None:intergenic 50.0%
CCAACACCTATTCAGGTGCA+AGG + chr1.2:12898008-12898027 MS.gene33874:CDS 50.0%
CCTTGCACCTGAATAGGTGT+TGG - chr1.2:12898011-12898030 None:intergenic 50.0%
CGTGGCAATTCATGGAGACA+AGG + chr1.2:12898775-12898794 MS.gene33874:CDS 50.0%
GATCATGATGGCAATGCAGG+AGG + chr1.2:12898115-12898134 MS.gene33874:CDS 50.0%
GCTGATCGATTGGTAGATCC+TGG + chr1.2:12898434-12898453 MS.gene33874:CDS 50.0%
GGAATTGCTTTCACGGGTTC+TGG + chr1.2:12898065-12898084 MS.gene33874:CDS 50.0%
GGCTGAGCTGATTGATCCAA+TGG + chr1.2:12899087-12899106 MS.gene33874:CDS 50.0%
GTCCATAGGATTGGACGTAC+TGG + chr1.2:12898947-12898966 MS.gene33874:CDS 50.0%
TGTGCAGCCAACACCTATTC+AGG + chr1.2:12898001-12898020 MS.gene33874:CDS 50.0%
TTGTCAATGTGGGACGTGCA+GGG + chr1.2:12898582-12898601 MS.gene33874:CDS 50.0%
! ACAGAGGATTCCCCCAGTTT+TGG + chr1.2:12899066-12899085 MS.gene33874:CDS 50.0%
! CACGGGTTCTGGCAAAACTT+TGG + chr1.2:12898076-12898095 MS.gene33874:CDS 50.0%
! GGGTTTTCTGTAGGCACTCA+AGG - chr1.2:12898673-12898692 None:intergenic 50.0%
AAAGGAGGCTTCCACCCAGT+AGG - chr1.2:12897828-12897847 None:intergenic 55.0%
ACGAACCATTGCCTACTGGG+TGG + chr1.2:12897814-12897833 MS.gene33874:CDS 55.0%
ACGTAGTTGTTGCCACGCCT+GGG + chr1.2:12898342-12898361 MS.gene33874:CDS 55.0%
CTTGAAAGGAGTGGAAGCCG+TGG + chr1.2:12898757-12898776 MS.gene33874:CDS 55.0%
GGAAGCCCTTGCACCTGAAT+AGG - chr1.2:12898017-12898036 None:intergenic 55.0%
GGACCTTCACCGGGAACTAT+AGG - chr1.2:12898152-12898171 None:intergenic 55.0%
GTGGAAGCCGTGGCAATTCA+TGG + chr1.2:12898767-12898786 MS.gene33874:CDS 55.0%
GTTCCCGGTGAAGGTCCATT+TGG + chr1.2:12898155-12898174 MS.gene33874:CDS 55.0%
TGTCAATGTGGGACGTGCAG+GGG + chr1.2:12898583-12898602 MS.gene33874:CDS 55.0%
! CAGGAGGTGGGGTTTTCTGT+AGG - chr1.2:12898682-12898701 None:intergenic 55.0%
! TGTTGGCTGCACAATCCCCT+TGG - chr1.2:12897994-12898013 None:intergenic 55.0%
!! TGTGGTAATTGTGGCGGGCT+TGG + chr1.2:12899151-12899170 MS.gene33874:CDS 55.0%
ATCCTTCAACCTCCCAGGCG+TGG - chr1.2:12898357-12898376 None:intergenic 60.0%
CACGTAGTTGTTGCCACGCC+TGG + chr1.2:12898341-12898360 MS.gene33874:CDS 60.0%
GGCTTCCACCCAGTAGGCAA+TGG - chr1.2:12897822-12897841 None:intergenic 60.0%
GTCCATCGAGGGAATTGGCG+AGG + chr1.2:12898189-12898208 MS.gene33874:CDS 60.0%
TAGTTGTTGCCACGCCTGGG+AGG + chr1.2:12898345-12898364 MS.gene33874:CDS 60.0%
TGCCTCGCCAATTCCCTCGA+TGG - chr1.2:12898194-12898213 None:intergenic 60.0%
CAATGTGGGACGTGCAGGGG+CGG + chr1.2:12898586-12898605 MS.gene33874:CDS 65.0%
TGCCACGCCTGGGAGGTTGA+AGG + chr1.2:12898352-12898371 MS.gene33874:CDS 65.0%
ATGGCGCGACGCTTCGCCAC+AGG - chr1.2:12897549-12897568 None:intergenic 70.0%
!! TGTGGCGAAGCGTCGCGCCA+TGG + chr1.2:12897548-12897567 MS.gene33874:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 12897504 12899279 12897504 ID=MS.gene33874
chr1.2 mRNA 12897504 12899279 12897504 ID=MS.gene33874.t1;Parent=MS.gene33874
chr1.2 exon 12897504 12899279 12897504 ID=MS.gene33874.t1.exon1;Parent=MS.gene33874.t1
chr1.2 CDS 12897504 12899279 12897504 ID=cds.MS.gene33874.t1;Parent=MS.gene33874.t1
Gene Sequence

>MS.gene33874

ATGGTAGCTAAAATGGAAGAAGATGATTATGAGGAGTATATTCCTGTGGCGAAGCGTCGCGCCATGGAAGCTCAGAAGATTCTCCAACGCAAAGGGAAATTATCTGCTCCAATAGATGATGATTCTAGAAAACTAAGTGTGGTTGAAACTAAACCCAGTCTACTTGTTAAGGCCTCACAGTTAAAGAAAGAACAACCTGAGATTAGTGTTACAGAACAGATTGTTCAACAGGAGAAAGAGATGATTGAGAATTTGTCTGATAAAAAAACCCTTATGTCGGTTCGTGAATTAGCGAAAGGGATCACTTATAACGAACCATTGCCTACTGGGTGGAAGCCTCCTTTGCATATTAGAAGGATGACAAAGAAAGATTGTGATTTGATTCAAAAACAGTGGCATATAATTGTCAATGGTGAAGATATTCCACCCGCGATTAAGAATTTTAAGGATATGAGGTTTCCGGATCCTATTTTGAAGATGTTGAAAACCAAGGGGATTGTGCAGCCAACACCTATTCAGGTGCAAGGGCTTCCTGTTATTTTATCTGGGAGGGATATGATTGGAATTGCTTTCACGGGTTCTGGCAAAACTTTGGTTTTTGTGCTTCCAATGATCATGATGGCAATGCAGGAGGAAATTACGATGCCTATAGTTCCCGGTGAAGGTCCATTTGGTCTGATTATTTGTCCATCGAGGGAATTGGCGAGGCAAACTTATGAAGTCATTGAACAATTCTTGTTACCTTTGAAGGAATCTGGATATCCGGAGCTCAGACCTTTGCTTTGTATTGGTGGAATTGATACGAGATCACAGCTTGAGATTGTCAAGAAGGGGGTTCACGTAGTTGTTGCCACGCCTGGGAGGTTGAAGGATATGTTGGCCAAGAAGAAAATGAATCTTGATAACTGCAGGTATTTAACGTTAGATGAGGCTGATCGATTGGTAGATCCTGGATTTGAAGATGATATAAGAGAAGTTTTTGATCACTTCAAAGCTCAAAGGCAGACTCTCTTGTTTTCTGCTACTATGCCAACCAAAATTCAAAATTTTGCTAGAAGTGCTTTGGTAAAACCTATTATTGTCAATGTGGGACGTGCAGGGGCGGCGAATCTTGATGTTATTCAGGAAGTAGAGTATGTCAAACAAGAGGCAAAAATTGTTTACCTCCTTGAGTGCCTACAGAAAACCCCACCTCCTGTTTTGATATTTTGTGAGAATAAGGCAGATGTCGATGACATTCATGAGTATCTTCTCTTGAAAGGAGTGGAAGCCGTGGCAATTCATGGAGACAAGGATCAGGAAGATAGAGAGTATGCTATTTCGTCATTTAAGACTGGAAAGAAGGATGTGTTGGTTGCAACTGATATTGCATCCAAAGGCTTGGATTTTCCTGATATTCAACATGTCATTAACTACGACGTGCCTGCTGAAATTGAAAATTATGTCCATAGGATTGGACGTACTGGAAGATGTGGCAAAACTGGTATAGCGACAACATTTATAAACAAGAACCAAACTGAGACAACATTGCTTGATTTGAAACACCTTTTGCGAGAAGCAAAACAGAGGATTCCCCCAGTTTTGGCTGAGCTGATTGATCCAATGGAAGATGATGAAGAAATCACTGGAATAAGTGGTATCAAGGGATGTGGTAATTGTGGCGGGCTTGGTCATCGTATCCTTGATTGTCCAAAATTAGAACATCAGAATAGAATGGAAAATGCAAATAATAGGAAGGATTCTTTTGGATCTGGAGGTTACAAAGGAGAAATTTGA
Protein sequence

>MS.gene33874.t1

MVAKMEEDDYEEYIPVAKRRAMEAQKILQRKGKLSAPIDDDSRKLSVVETKPSLLVKASQLKKEQPEISVTEQIVQQEKEMIENLSDKKTLMSVRELAKGITYNEPLPTGWKPPLHIRRMTKKDCDLIQKQWHIIVNGEDIPPAIKNFKDMRFPDPILKMLKTKGIVQPTPIQVQGLPVILSGRDMIGIAFTGSGKTLVFVLPMIMMAMQEEITMPIVPGEGPFGLIICPSRELARQTYEVIEQFLLPLKESGYPELRPLLCIGGIDTRSQLEIVKKGVHVVVATPGRLKDMLAKKKMNLDNCRYLTLDEADRLVDPGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPIIVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIHEYLLLKGVEAVAIHGDKDQEDREYAISSFKTGKKDVLVATDIASKGLDFPDIQHVINYDVPAEIENYVHRIGRTGRCGKTGIATTFINKNQTETTLLDLKHLLREAKQRIPPVLAELIDPMEDDEEITGISGIKGCGNCGGLGHRILDCPKLEHQNRMENANNRKDSFGSGGYKGEI