AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34002

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34002.t1 MTR_1g095810 99.660 589 2 0 1 589 1 589 0.0 1203
MS.gene34002.t1 MTR_1g026830 94.037 587 34 1 3 589 6 591 0.0 1128
MS.gene34002.t1 MTR_2g100230 40.431 418 236 5 115 529 66 473 3.50e-100 313
MS.gene34002.t1 MTR_3g467550 38.614 404 226 5 109 498 77 472 1.98e-96 305
MS.gene34002.t1 MTR_4g105100 40.334 419 237 5 115 530 172 580 1.86e-94 302
MS.gene34002.t1 MTR_3g083690 38.889 414 227 7 134 530 134 538 1.37e-93 300
MS.gene34002.t1 MTR_7g109720 43.468 421 222 8 115 530 202 611 1.52e-93 304
MS.gene34002.t1 MTR_1g054630 39.803 407 224 7 134 526 143 542 2.53e-91 293
MS.gene34002.t1 MTR_6g005550 39.563 412 229 8 124 529 144 541 2.62e-91 296
MS.gene34002.t1 MTR_3g467550 38.250 400 202 6 109 498 77 441 2.98e-90 288
MS.gene34002.t1 MTR_4g134170 36.449 428 247 4 118 527 260 680 7.20e-90 292
MS.gene34002.t1 MTR_6g005320 36.574 432 247 5 114 527 218 640 1.10e-88 288
MS.gene34002.t1 MTR_4g114120 38.537 410 228 5 136 528 131 533 1.26e-86 283
MS.gene34002.t1 MTR_2g007000 36.726 452 263 9 115 558 474 910 1.74e-83 283
MS.gene34002.t1 MTR_2g032630 39.709 413 231 7 123 530 474 873 1.03e-82 281
MS.gene34002.t1 MTR_3g467550 36.675 379 218 5 109 473 77 447 5.91e-82 265
MS.gene34002.t1 MTR_5g027840 38.481 395 226 8 136 523 95 479 2.44e-78 257
MS.gene34002.t1 MTR_4g105100 39.368 348 199 4 115 459 172 510 2.09e-71 239
MS.gene34002.t1 MTR_5g090120 38.889 360 197 9 155 506 91 435 2.61e-64 221
MS.gene34002.t1 MTR_4g079320 33.498 406 246 7 146 541 20 411 2.78e-64 225
MS.gene34002.t1 MTR_6g033070 31.616 427 275 9 124 546 120 533 2.93e-63 217
MS.gene34002.t1 MTR_7g056830 33.777 376 225 6 137 505 2 360 5.82e-63 214
MS.gene34002.t1 MTR_6g082080 33.777 376 225 6 137 505 2 360 6.67e-63 214
MS.gene34002.t1 MTR_3g449250 37.989 358 199 9 155 504 100 442 8.92e-63 216
MS.gene34002.t1 MTR_3g449250 37.989 358 199 9 155 504 100 442 1.48e-62 216
MS.gene34002.t1 MTR_3g049990 35.342 365 216 6 145 502 152 503 5.56e-61 216
MS.gene34002.t1 MTR_7g100820 34.135 416 243 11 125 529 110 505 1.57e-59 209
MS.gene34002.t1 MTR_2g041140 34.771 371 223 6 145 509 141 498 2.30e-59 211
MS.gene34002.t1 MTR_2g066050 34.239 368 215 6 145 502 53 403 2.01e-57 201
MS.gene34002.t1 MTR_2g039960 30.848 389 250 8 145 530 41 413 7.60e-55 191
MS.gene34002.t1 MTR_4g125970 30.848 389 250 8 145 530 41 413 1.47e-54 191
MS.gene34002.t1 MTR_2g438110 31.458 391 249 7 143 530 32 406 1.90e-54 190
MS.gene34002.t1 MTR_4g125970 30.848 389 250 8 145 530 58 430 3.90e-54 190
MS.gene34002.t1 MTR_2g099620 30.513 390 252 8 144 530 24 397 4.47e-53 186
MS.gene34002.t1 MTR_2g099620 30.513 390 252 8 144 530 40 413 5.84e-53 186
MS.gene34002.t1 MTR_4g112340 35.629 334 196 9 163 488 115 437 1.12e-52 189
MS.gene34002.t1 MTR_8g018400 29.820 389 254 8 145 530 41 413 4.06e-52 184
MS.gene34002.t1 MTR_3g071620 32.419 401 231 13 146 517 15 404 4.59e-52 188
MS.gene34002.t1 MTR_2g055850 31.492 362 233 6 144 499 90 442 9.27e-52 187
MS.gene34002.t1 MTR_8g027780 34.511 368 213 10 146 493 102 461 5.44e-50 182
MS.gene34002.t1 MTR_7g076820 28.756 386 258 5 146 530 151 520 2.78e-49 179
MS.gene34002.t1 MTR_7g076830 29.016 386 257 5 146 530 144 513 3.19e-49 179
MS.gene34002.t1 MTR_1g102410 32.678 407 218 11 132 504 34 418 4.70e-49 181
MS.gene34002.t1 MTR_2g043360 28.365 416 218 8 163 505 60 468 5.43e-49 180
MS.gene34002.t1 MTR_7g076800 28.756 386 258 5 146 530 142 511 5.56e-49 178
MS.gene34002.t1 MTR_6g056080 32.533 375 229 9 140 504 101 461 8.57e-49 181
MS.gene34002.t1 MTR_4g059460 28.780 410 212 9 163 499 63 465 1.07e-48 179
MS.gene34002.t1 MTR_8g027775 32.391 389 224 11 133 493 80 457 2.61e-48 177
MS.gene34002.t1 MTR_4g010565 30.939 362 227 8 146 500 125 470 3.40e-48 176
MS.gene34002.t1 MTR_3g107420 30.641 359 232 7 146 500 48 393 3.98e-47 171
MS.gene34002.t1 MTR_1g012420 29.319 382 253 7 146 523 48 416 1.05e-46 170
MS.gene34002.t1 MTR_6g039840 29.306 389 235 9 137 501 102 474 1.90e-43 165
MS.gene34002.t1 MTR_8g027770 32.865 356 210 8 156 493 212 556 6.39e-43 164
MS.gene34002.t1 MTR_8g097000 33.148 359 217 10 156 499 299 649 1.46e-42 163
MS.gene34002.t1 MTR_2g063080 27.446 419 247 8 137 503 101 514 2.13e-41 157
MS.gene34002.t1 MTR_5g092560 33.140 344 211 9 164 499 95 427 3.37e-41 159
MS.gene34002.t1 MTR_1g061970 31.139 395 212 15 166 521 102 475 2.14e-40 154
MS.gene34002.t1 MTR_8g027780 32.945 343 202 10 146 468 102 436 9.66e-40 151
MS.gene34002.t1 MTR_7g057250 27.902 448 245 11 146 523 164 603 3.71e-39 154
MS.gene34002.t1 MTR_4g068990 31.662 379 234 13 141 510 73 435 1.71e-37 148
MS.gene34002.t1 MTR_1g083130 26.506 415 238 9 144 506 25 424 4.19e-36 143
MS.gene34002.t1 MTR_4g112320 35.294 238 143 6 259 488 21 255 4.41e-35 137
MS.gene34002.t1 MTR_3g118120 26.494 385 246 11 143 499 130 505 7.42e-34 135
MS.gene34002.t1 MTR_4g049910 29.737 380 214 10 146 499 56 408 1.02e-33 134
MS.gene34002.t1 MTR_2g043360 26.857 350 173 7 239 505 1 350 9.54e-33 132
MS.gene34002.t1 MTR_0915s0010 28.425 292 193 4 162 452 6 282 8.78e-32 124
MS.gene34002.t1 MTR_1g046570 29.603 277 181 8 264 530 126 398 3.19e-30 123
MS.gene34002.t1 MTR_6g039840 28.947 304 177 8 221 501 11 298 5.59e-29 121
MS.gene34002.t1 MTR_5g069330 26.287 369 240 12 143 500 5 352 8.18e-29 119
MS.gene34002.t1 MTR_5g069330 26.287 369 240 12 143 500 1 348 1.12e-28 119
MS.gene34002.t1 MTR_4g131750 28.426 394 219 13 135 488 72 442 5.57e-28 117
MS.gene34002.t1 MTR_4g459350 32.512 203 111 4 163 346 60 255 2.97e-26 108
MS.gene34002.t1 MTR_8g018370 23.823 361 231 7 175 530 3 324 1.38e-24 105
MS.gene34002.t1 MTR_4g077370 33.810 210 112 6 145 346 250 440 8.56e-24 105
MS.gene34002.t1 MTR_4g077370 30.806 211 102 6 145 346 2 177 1.33e-18 89.0
MS.gene34002.t1 MTR_5g092560 32.178 202 131 4 304 499 15 216 1.30e-23 105
MS.gene34002.t1 MTR_4g068990 31.925 213 136 6 304 510 15 224 2.46e-21 98.2
MS.gene34002.t1 MTR_8g042690 24.425 348 228 9 168 499 41 369 3.53e-21 97.1
MS.gene34002.t1 MTR_3g107420 30.049 203 127 5 146 346 48 237 1.99e-20 92.8
MS.gene34002.t1 MTR_8g032230 35.294 170 96 4 137 305 2 158 5.42e-20 87.8
MS.gene34002.t1 MTR_1g022305 25.731 342 176 11 167 504 26 293 7.69e-20 93.2
MS.gene34002.t1 MTR_5g077690 25.393 382 218 12 182 503 70 444 2.25e-19 92.0
MS.gene34002.t1 MTR_3g063040 23.810 357 230 10 153 496 2 329 2.26e-13 73.9
MS.gene34002.t1 MTR_8g080160 33.051 118 74 1 388 500 480 597 9.93e-13 71.6
MS.gene34002.t1 MTR_8g062720 25.743 202 132 4 320 505 345 544 4.09e-12 69.3
MS.gene34002.t1 MTR_0427s0010 28.302 212 115 6 314 508 1 192 1.03e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34002.t1 AT5G51280 87.097 589 74 1 1 589 5 591 0.0 1061
MS.gene34002.t1 AT4G33370 75.382 589 98 1 1 589 1 542 0.0 910
MS.gene34002.t1 AT1G55150 39.277 415 239 5 118 529 73 477 9.55e-100 312
MS.gene34002.t1 AT1G55150 39.277 415 239 5 118 529 98 502 6.24e-99 311
MS.gene34002.t1 AT5G63120 41.190 420 232 7 115 530 136 544 2.35e-97 309
MS.gene34002.t1 AT3G58510 38.272 405 231 6 134 526 141 538 1.41e-93 300
MS.gene34002.t1 AT3G58510 38.272 405 231 6 134 526 141 538 1.41e-93 300
MS.gene34002.t1 AT3G58510 38.272 405 231 6 134 526 141 538 1.41e-93 300
MS.gene34002.t1 AT2G47330 40.708 452 251 9 85 530 168 608 3.56e-93 302
MS.gene34002.t1 AT2G33730 37.617 428 242 4 118 527 287 707 1.07e-92 300
MS.gene34002.t1 AT3G58570 38.875 409 229 7 132 526 134 535 3.62e-92 297
MS.gene34002.t1 AT2G42520 37.897 409 233 6 132 526 147 548 5.95e-92 296
MS.gene34002.t1 AT2G42520 37.897 409 233 6 132 526 147 548 5.95e-92 296
MS.gene34002.t1 AT2G42520 37.897 409 233 6 132 526 147 548 5.95e-92 296
MS.gene34002.t1 AT1G20920 36.807 451 268 7 115 560 162 600 5.29e-90 296
MS.gene34002.t1 AT1G20920 36.807 451 268 7 115 560 500 938 4.26e-88 296
MS.gene34002.t1 AT1G20920 36.807 451 268 7 115 560 500 938 4.26e-88 296
MS.gene34002.t1 AT1G20920 36.807 451 268 7 115 560 500 938 4.26e-88 296
MS.gene34002.t1 AT1G20920 36.807 451 268 7 115 560 500 938 4.26e-88 296
MS.gene34002.t1 AT1G20920 36.807 451 268 7 115 560 500 938 4.26e-88 296
MS.gene34002.t1 AT1G20920 36.807 451 268 7 115 560 500 938 4.26e-88 296
MS.gene34002.t1 AT3G01540 39.416 411 227 8 127 530 141 536 1.64e-87 284
MS.gene34002.t1 AT3G01540 39.416 411 227 8 127 530 141 536 1.64e-87 284
MS.gene34002.t1 AT3G01540 39.416 411 227 8 127 530 141 536 1.64e-87 284
MS.gene34002.t1 AT3G01540 39.416 411 227 8 127 530 141 536 1.74e-87 284
MS.gene34002.t1 AT5G14610 38.765 405 228 7 132 530 149 539 7.11e-86 280
MS.gene34002.t1 AT5G14610 38.765 405 228 7 132 530 149 539 8.42e-86 280
MS.gene34002.t1 AT5G14610 38.765 405 228 7 132 530 157 547 1.51e-85 280
MS.gene34002.t1 AT3G06480 39.709 413 231 7 123 530 414 813 1.38e-80 275
MS.gene34002.t1 AT1G31970 38.462 416 220 12 127 523 95 493 5.23e-80 262
MS.gene34002.t1 AT5G14610 37.440 414 237 8 125 530 208 607 1.62e-78 262
MS.gene34002.t1 AT5G14610 39.050 379 211 7 158 530 44 408 1.64e-78 257
MS.gene34002.t1 AT5G14610 39.050 379 211 7 158 530 44 408 1.64e-78 257
MS.gene34002.t1 AT3G09620 34.581 454 245 9 115 560 367 776 6.47e-75 258
MS.gene34002.t1 AT3G09620 34.581 454 245 9 115 560 368 777 7.02e-75 258
MS.gene34002.t1 AT5G63120 39.828 349 196 6 115 459 136 474 6.48e-73 241
MS.gene34002.t1 AT5G63120 39.828 349 196 6 115 459 136 474 1.04e-72 241
MS.gene34002.t1 AT5G63120 39.828 349 196 6 115 459 136 474 1.04e-72 241
MS.gene34002.t1 AT5G63120 39.828 349 196 6 115 459 136 474 1.04e-72 241
MS.gene34002.t1 AT1G28180 38.375 357 207 5 174 527 3 349 2.16e-71 234
MS.gene34002.t1 AT1G77030 34.010 394 242 6 146 535 30 409 1.50e-65 229
MS.gene34002.t1 AT5G14610 35.754 358 211 6 128 479 153 497 8.26e-65 221
MS.gene34002.t1 AT5G60990 33.867 375 236 4 137 505 2 370 1.04e-64 219
MS.gene34002.t1 AT1G16280 33.257 436 255 11 145 567 59 471 1.64e-64 219
MS.gene34002.t1 AT3G02065 32.220 419 266 7 119 529 83 491 7.31e-64 218
MS.gene34002.t1 AT3G02065 32.220 419 266 7 119 529 83 491 7.31e-64 218
MS.gene34002.t1 AT3G09720 33.488 430 254 11 111 529 105 513 8.01e-62 214
MS.gene34002.t1 AT3G09720 33.488 430 254 11 111 529 105 513 8.01e-62 214
MS.gene34002.t1 AT3G06480 38.286 350 199 6 124 468 415 752 2.15e-61 217
MS.gene34002.t1 AT4G16630 33.824 408 237 9 145 536 168 558 4.34e-61 216
MS.gene34002.t1 AT3G22330 35.311 354 214 7 155 504 115 457 1.21e-60 212
MS.gene34002.t1 AT5G26742 32.990 388 220 6 135 506 98 461 1.63e-58 209
MS.gene34002.t1 AT5G26742 32.990 388 220 6 135 506 98 461 1.65e-58 209
MS.gene34002.t1 AT3G22310 34.626 361 221 7 148 504 120 469 7.39e-58 204
MS.gene34002.t1 AT3G22310 34.626 361 221 7 148 504 120 469 9.62e-58 204
MS.gene34002.t1 AT5G65900 33.428 353 214 7 144 488 154 493 7.97e-57 202
MS.gene34002.t1 AT3G13920 30.513 390 252 8 144 530 39 412 2.10e-56 196
MS.gene34002.t1 AT3G13920 30.513 390 252 8 144 530 29 402 2.15e-56 195
MS.gene34002.t1 AT1G51380 31.122 392 248 6 142 530 20 392 3.63e-56 194
MS.gene34002.t1 AT3G18600 33.425 362 226 6 131 488 77 427 1.14e-55 197
MS.gene34002.t1 AT5G26742 32.115 383 224 6 179 545 44 406 1.84e-55 199
MS.gene34002.t1 AT3G13920 31.183 372 237 8 162 530 52 407 3.75e-55 192
MS.gene34002.t1 AT3G19760 30.964 394 249 7 142 530 33 408 5.18e-55 192
MS.gene34002.t1 AT3G13920 30.130 385 251 7 144 525 39 408 2.62e-54 190
MS.gene34002.t1 AT1G72730 29.820 389 254 8 145 530 42 414 3.63e-54 190
MS.gene34002.t1 AT3G13920 30.130 385 251 7 144 525 43 412 3.65e-54 190
MS.gene34002.t1 AT1G54270 30.612 392 249 8 144 530 39 412 5.23e-53 186
MS.gene34002.t1 AT3G02065 31.593 364 233 6 172 529 1 354 5.61e-53 185
MS.gene34002.t1 AT1G71370 32.086 374 232 9 139 499 9 373 6.10e-53 190
MS.gene34002.t1 AT1G54270 31.283 374 234 8 162 530 52 407 8.76e-52 183
MS.gene34002.t1 AT5G62190 32.283 381 234 9 140 510 95 461 5.31e-51 187
MS.gene34002.t1 AT2G45810 30.618 356 231 4 146 500 156 496 7.18e-50 181
MS.gene34002.t1 AT5G05450 30.990 384 227 10 144 499 9 382 1.01e-49 182
MS.gene34002.t1 AT3G61240 30.337 356 230 5 146 499 126 465 1.22e-49 179
MS.gene34002.t1 AT3G61240 30.337 356 230 5 146 499 126 465 1.22e-49 179
MS.gene34002.t1 AT4G00660 29.737 380 250 5 146 524 133 496 1.50e-49 179
MS.gene34002.t1 AT4G00660 29.737 380 250 5 146 524 133 496 1.50e-49 179
MS.gene34002.t1 AT5G11170 30.789 380 231 11 134 499 31 392 2.87e-48 174
MS.gene34002.t1 AT5G11200 30.789 380 231 11 134 499 31 392 2.87e-48 174
MS.gene34002.t1 AT2G40700 29.710 414 205 10 163 499 50 454 1.11e-46 174
MS.gene34002.t1 AT1G12770 27.725 422 242 9 138 503 101 515 2.75e-45 169
MS.gene34002.t1 AT1G12770 27.725 422 242 9 138 503 101 515 2.75e-45 169
MS.gene34002.t1 AT5G54910 33.075 387 230 12 143 519 70 437 8.28e-44 167
MS.gene34002.t1 AT5G63630 31.429 350 223 6 156 493 66 410 1.16e-43 164
MS.gene34002.t1 AT5G11170 32.209 326 194 10 183 499 2 309 1.70e-43 159
MS.gene34002.t1 AT5G11200 28.637 433 233 14 134 510 31 443 3.87e-43 161
MS.gene34002.t1 AT5G63630 31.429 350 223 6 156 493 260 604 9.94e-43 164
MS.gene34002.t1 AT2G07750 31.281 406 243 10 111 493 340 732 1.25e-42 164
MS.gene34002.t1 AT5G08610 32.022 356 213 7 156 493 394 738 2.01e-42 164
MS.gene34002.t1 AT4G09730 28.646 384 232 10 143 501 111 477 7.51e-42 160
MS.gene34002.t1 AT5G11200 28.082 438 226 13 134 499 31 451 1.49e-41 157
MS.gene34002.t1 AT1G63250 31.116 421 255 12 156 556 340 745 1.77e-41 160
MS.gene34002.t1 AT5G08620 30.806 422 254 13 156 556 92 496 4.75e-41 157
MS.gene34002.t1 AT1G71280 30.838 334 190 10 137 463 11 310 1.71e-37 142
MS.gene34002.t1 AT3G53110 29.319 382 233 13 133 493 80 445 5.16e-37 144
MS.gene34002.t1 AT1G71280 30.473 338 194 10 137 467 11 314 8.42e-37 143
MS.gene34002.t1 AT3G06980 28.460 383 238 13 144 499 374 747 5.52e-33 135
MS.gene34002.t1 AT4G34910 28.101 395 225 10 144 489 46 430 5.80e-33 134
MS.gene34002.t1 AT3G16840 27.209 430 232 11 146 504 193 612 6.92e-32 132
MS.gene34002.t1 AT3G16840 27.209 430 232 11 146 504 192 611 7.50e-32 131
MS.gene34002.t1 AT1G51380 29.044 272 175 5 142 410 20 276 2.31e-28 115
MS.gene34002.t1 AT5G19210 26.579 380 243 11 134 488 66 434 1.77e-27 116
MS.gene34002.t1 AT1G51380 30.093 216 137 2 142 357 20 221 1.27e-25 107
MS.gene34002.t1 AT1G51380 30.093 216 137 2 142 357 20 221 1.76e-25 107
MS.gene34002.t1 AT4G15850 25.182 413 233 12 157 503 44 446 2.61e-23 104
MS.gene34002.t1 AT5G19210 26.180 233 148 7 279 488 46 277 3.27e-16 80.5
MS.gene34002.t1 AT5G19210 26.180 233 148 7 279 488 46 277 3.27e-16 80.5
MS.gene34002.t1 AT1G28180 36.885 122 71 1 174 295 3 118 5.21e-15 72.0
MS.gene34002.t1 AT1G27880 23.955 359 228 12 171 513 274 603 2.97e-12 70.1
MS.gene34002.t1 AT1G27880 23.955 359 228 12 171 513 274 603 3.18e-12 70.1
MS.gene34002.t1 AT4G35740 20.800 375 241 13 140 488 5 349 3.83e-12 69.7

Find 158 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTAGCAAAGTTCTGAATTT+TGG 0.149090 1.4:-74428005 None:intergenic
AATAATAGAAAGGATTATTT+TGG 0.164744 1.4:+74428697 MS.gene34002:CDS
GTATGCCATTTCATCATTTA+AGG 0.167719 1.4:+74428282 MS.gene34002:CDS
CTCTTCTGATGTTCTAATTT+AGG 0.202029 1.4:-74428661 None:intergenic
CAGTGGCATATTATTGTTAA+TGG 0.225650 1.4:+74427362 MS.gene34002:CDS
CGTCCCACATTGACAATAAT+AGG 0.278247 1.4:-74428043 None:intergenic
ATATCCTTAAAATTCTTAAT+CGG 0.278731 1.4:-74427401 None:intergenic
GGCTGATCGATTGGTCGATT+TGG 0.290280 1.4:+74427901 MS.gene34002:CDS
GGCAAATCTTGATGTTATTC+AGG 0.295497 1.4:+74428075 MS.gene34002:CDS
TGCCTAGCCAATTCCCTTGA+TGG 0.301873 1.4:-74427659 None:intergenic
GCTGATCGATTGGTCGATTT+GGG 0.307880 1.4:+74427902 MS.gene34002:CDS
TGTGCAGCCAACGCCTATTC+AGG 0.330764 1.4:+74427469 MS.gene34002:CDS
TTGATGACATGTTGAATATC+AGG 0.337184 1.4:-74428361 None:intergenic
TGTGCATATTGTGGCGGGCT+TGG 0.337993 1.4:+74428619 MS.gene34002:CDS
ATTATGATGCCGATTGTTCC+TGG 0.348651 1.4:+74427608 MS.gene34002:CDS
TCCCTCCCAGATAAAATAAC+TGG 0.356395 1.4:-74427503 None:intergenic
AGCTTCCATGGCGCGACGCT+TGG 0.358137 1.4:-74427018 None:intergenic
TATACTGAGCCTTTGCCTAC+TGG 0.358544 1.4:+74427278 MS.gene34002:CDS
TATTTGTCCATCAAGGGAAT+TGG 0.368905 1.4:+74427652 MS.gene34002:CDS
TTGGCATAGTAGCAGAAAAT+AGG 0.371521 1.4:-74427982 None:intergenic
TTCTCACAAAATATAAGAAC+AGG 0.372394 1.4:-74428166 None:intergenic
TATCCTTAAAATTCTTAATC+GGG 0.374649 1.4:-74427400 None:intergenic
ATCAGGAAAATCTAAACCTT+TGG 0.383694 1.4:-74428344 None:intergenic
TCAACCAGCTCAGCCAAAAC+AGG 0.388073 1.4:-74428547 None:intergenic
ACCGGAAGATGTGGCAAAAC+TGG 0.393311 1.4:+74428433 MS.gene34002:CDS
TCCAGCCTTAAATGATGAAA+TGG 0.394298 1.4:-74428287 None:intergenic
GTCTGATTATTTGTCCATCA+AGG 0.395594 1.4:+74427645 MS.gene34002:CDS
GAACTTTGCTAGAAGTGCTT+TGG 0.398689 1.4:+74428015 MS.gene34002:CDS
GGAAGACCCTGCACCTGAAT+AGG 0.399752 1.4:-74427482 None:intergenic
GAAAATTATGTCCATAGGAT+TGG 0.415181 1.4:+74428406 MS.gene34002:CDS
ATTTCTTCATTATCTTCCAT+TGG 0.416148 1.4:-74428571 None:intergenic
GTGGAAGCCGTGGCTATTCA+TGG 0.417643 1.4:+74428235 MS.gene34002:CDS
CGCAATGGAAGAAGATGATT+CGG 0.423328 1.4:+74427076 MS.gene34002:CDS
CATATAGTTGTTGCAACTCC+CGG 0.423420 1.4:+74427809 MS.gene34002:CDS
CTCAGGCCTTTGCTTTGTAT+CGG 0.425118 1.4:+74427740 MS.gene34002:CDS
ATTGTCAATGTGGGACGTGC+AGG 0.426137 1.4:+74428049 MS.gene34002:CDS
TAAGCCAAGTCTACTTGTTA+AGG 0.431259 1.4:+74427121 MS.gene34002:CDS
ATGGCGCGACGCTTGGCCAC+AGG 0.433274 1.4:-74427011 None:intergenic
CAAGCAATGTTGTCTCACTT+TGG 0.433879 1.4:-74428483 None:intergenic
AACATCTTCAAAATCGGATC+CGG 0.439066 1.4:-74427431 None:intergenic
ATACTGAGCCTTTGCCTACT+GGG 0.441611 1.4:+74427279 MS.gene34002:CDS
GGAAGAAGAAGATGATTATG+AGG 0.447944 1.4:+74426980 MS.gene34002:CDS
TTGTTACCTTTGAAGGAAGC+TGG 0.448833 1.4:+74427707 MS.gene34002:CDS
CTAATTCACGAACCGACATA+AGG 0.449435 1.4:-74427241 None:intergenic
TCTGTTTAGCTTCTTGCAAA+AGG 0.453849 1.4:-74428516 None:intergenic
GCCATTTCATCATTTAAGGC+TGG 0.454604 1.4:+74428286 MS.gene34002:CDS
GTTCCTGGGGAAGGTCCGTT+TGG 0.454718 1.4:+74427623 MS.gene34002:CDS
TTCCGGTACGTCCAATCCTA+TGG 0.458107 1.4:-74428417 None:intergenic
ATCCTTTCTATTATTTGCAA+TGG 0.461654 1.4:-74428689 None:intergenic
TACGGGTTCTGGTAAAACTT+TGG 0.465912 1.4:+74427544 MS.gene34002:CDS
TTTGAAGGAAGCTGGATATC+CGG 0.466679 1.4:+74427715 MS.gene34002:CDS
GCCAACGCCTATTCAGGTGC+AGG 0.470151 1.4:+74427475 MS.gene34002:CDS
TAATTCACGAACCGACATAA+GGG 0.473252 1.4:-74427240 None:intergenic
AAGCTGGATATCCGGAGCTC+AGG 0.476787 1.4:+74427723 MS.gene34002:CDS
CATGAGTATCTTCTCTTGAA+AGG 0.476814 1.4:+74428211 MS.gene34002:CDS
TTATCTGGGAGGGATATGAT+TGG 0.476899 1.4:+74427512 MS.gene34002:CDS
CGTTGGCTGCACAATCCCCT+TGG 0.477106 1.4:-74427459 None:intergenic
TATTCATGGAGGCAAGGATC+AGG 0.479994 1.4:+74428249 MS.gene34002:CDS
CTCAGTATAAGTAATCCCTT+TGG 0.481450 1.4:-74427264 None:intergenic
TGTTCTGTGACACTGATCTC+AGG 0.484362 1.4:-74427167 None:intergenic
GGACCTTCCCCAGGAACAAT+CGG 0.494587 1.4:-74427617 None:intergenic
TGTGGCCAAGCGTCGCGCCA+TGG 0.495459 1.4:+74427013 MS.gene34002:CDS
GAAGAAATCACAGGCATAAG+TGG 0.497165 1.4:+74428586 MS.gene34002:CDS
GGAGATGATTGAGAATTTGT+CGG 0.499681 1.4:+74427208 MS.gene34002:CDS
CAACATATCCTTCAACCTCC+CGG 0.501564 1.4:-74427828 None:intergenic
GATGATTATGATGGCAATGC+AGG 0.503462 1.4:+74427580 MS.gene34002:CDS
TGCAACTCCCGGGAGGTTGA+AGG 0.505159 1.4:+74427820 MS.gene34002:CDS
TTGAAGATGTTGAAAACCAA+GGG 0.508448 1.4:+74427443 MS.gene34002:CDS
CAAAGCAAAGGCCTGAGCTC+CGG 0.515925 1.4:-74427734 None:intergenic
TGTGCTTCCGATGATTATGA+TGG 0.517107 1.4:+74427571 MS.gene34002:CDS
TTATGATGCCGATTGTTCCT+GGG 0.517162 1.4:+74427609 MS.gene34002:CDS
ATGCCGATTGTTCCTGGGGA+AGG 0.520774 1.4:+74427614 MS.gene34002:CDS
ATCCTTAAAATTCTTAATCG+GGG 0.521926 1.4:-74427399 None:intergenic
AGGGAAAGCAACCGCCGCAA+TGG 0.523486 1.4:+74427061 MS.gene34002:CDS
AGGCCTTTGCTTTGTATCGG+TGG 0.524480 1.4:+74427743 MS.gene34002:CDS
GGCCATTGCAAATAATAGAA+AGG 0.524636 1.4:+74428687 MS.gene34002:CDS
GCAACTGATGTTGCATCCAA+AGG 0.525642 1.4:+74428328 MS.gene34002:CDS
GGATATCCAGCTTCCTTCAA+AGG 0.531513 1.4:-74427713 None:intergenic
GTTAGATGAGGCTGATCGAT+TGG 0.532301 1.4:+74427892 MS.gene34002:CDS
GGCTGAGCTGGTTGATCCAA+TGG 0.532926 1.4:+74428555 MS.gene34002:CDS
CACAGGCATAAGTGGTGTCA+AGG 0.533189 1.4:+74428594 MS.gene34002:CDS
ACAGCTTGAGATTGTCAAGA+AGG 0.534600 1.4:+74427781 MS.gene34002:CDS
GTCCATCAAGGGAATTGGCT+AGG 0.535331 1.4:+74427657 MS.gene34002:CDS
TTGTCAATGTGGGACGTGCA+GGG 0.535671 1.4:+74428050 MS.gene34002:CDS
ATATAGTTGTTGCAACTCCC+GGG 0.536020 1.4:+74427810 MS.gene34002:CDS
CAATGTGGGACGTGCAGGGG+CGG 0.538248 1.4:+74428054 MS.gene34002:CDS
AAATTGAAAATTATGTCCAT+AGG 0.540038 1.4:+74428401 MS.gene34002:CDS
TTTGAAGATGTTGAAAACCA+AGG 0.541708 1.4:+74427442 MS.gene34002:CDS
TCTGATTATTTGTCCATCAA+GGG 0.542604 1.4:+74427646 MS.gene34002:CDS
AGAAGATTCTACAACGCAAA+GGG 0.543952 1.4:+74427042 MS.gene34002:CDS
GTCCATAGGATTGGACGTAC+CGG 0.544392 1.4:+74428415 MS.gene34002:CDS
CGGTTCGTGAATTAGCCAAA+GGG 0.544464 1.4:+74427249 MS.gene34002:CDS
AGGGCTGTGCATATTGTGGC+GGG 0.545255 1.4:+74428614 MS.gene34002:CDS
TATAAGAAGGATGTCGAAGA+AGG 0.546105 1.4:+74427319 MS.gene34002:CDS
CCCTGCACCTGAATAGGCGT+TGG 0.547462 1.4:-74427476 None:intergenic
TAGAATCTTCTGAGCTTCCA+TGG 0.548818 1.4:-74427030 None:intergenic
ACAGATTGTTCAACAAGAGA+AGG 0.551879 1.4:+74427187 MS.gene34002:CDS
CGGGAGGTTGAAGGATATGT+TGG 0.553032 1.4:+74427829 MS.gene34002:CDS
AACATATCCTTCAACCTCCC+GGG 0.553168 1.4:-74427827 None:intergenic
AGCCTCCTTTGCATATAAGA+AGG 0.556618 1.4:+74427306 MS.gene34002:CDS
TTGATCACTTCAAAGCTCAA+AGG 0.557864 1.4:+74427951 MS.gene34002:CDS
GACATCCTTCTTATATGCAA+AGG 0.558548 1.4:-74427311 None:intergenic
GGACAAATAATCAGACCAAA+CGG 0.559558 1.4:-74427638 None:intergenic
TGCATTGCCATCATAATCAT+CGG 0.564463 1.4:-74427578 None:intergenic
GGCTTCCACCCAGTAGGCAA+AGG 0.565662 1.4:-74427287 None:intergenic
GGCTGGAAAGAAAGATGTGT+TGG 0.568095 1.4:+74428303 MS.gene34002:CDS
TGGCGCGACGCTTGGCCACA+GGG 0.572510 1.4:-74427010 None:intergenic
AAAACCTATTATTGTCAATG+TGG 0.574587 1.4:+74428039 MS.gene34002:CDS
GTGATTTGATTCAGAAACAG+TGG 0.579699 1.4:+74427345 MS.gene34002:CDS
GTTCTAATTTAGGACAATCA+CGG 0.582056 1.4:-74428651 None:intergenic
CAGCTTGAGATTGTCAAGAA+GGG 0.585138 1.4:+74427782 MS.gene34002:CDS
CAACCAGCTCAGCCAAAACA+GGG 0.585303 1.4:-74428546 None:intergenic
AAGGGCTGTGCATATTGTGG+CGG 0.588782 1.4:+74428613 MS.gene34002:CDS
GATGCCTTAACAAGTAGACT+TGG 0.589716 1.4:-74427125 None:intergenic
TTGGATCTGGAGGTTACCGA+GGG 0.589965 1.4:+74428716 MS.gene34002:CDS
ATCATCTTCTTCCATTGCGG+CGG 0.590603 1.4:-74427072 None:intergenic
TGTCAATGTGGGACGTGCAG+GGG 0.592163 1.4:+74428051 MS.gene34002:CDS
AAACCTATTATTGTCAATGT+GGG 0.592275 1.4:+74428040 MS.gene34002:CDS
GTCAAGGGCTGTGCATATTG+TGG 0.594696 1.4:+74428610 MS.gene34002:CDS
GAAGATAATGAAGAAATCAC+AGG 0.595506 1.4:+74428577 MS.gene34002:CDS
CGAATCATCTTCTTCCATTG+CGG 0.596138 1.4:-74427075 None:intergenic
TAGGTATTTAACGTTAGATG+AGG 0.597304 1.4:+74427880 MS.gene34002:CDS
ACAGGCATAAGTGGTGTCAA+GGG 0.600892 1.4:+74428595 MS.gene34002:CDS
AGACCAAACGGACCTTCCCC+AGG 0.602098 1.4:-74427626 None:intergenic
TCCTTAAAATTCTTAATCGG+GGG 0.602509 1.4:-74427398 None:intergenic
ATTGGACGTACCGGAAGATG+TGG 0.605046 1.4:+74428424 MS.gene34002:CDS
ACAGCTTTCAAATTTCCCCT+CGG 0.605453 1.4:-74428732 None:intergenic
AAATGAATCTTGATAACTGT+AGG 0.605954 1.4:+74427861 MS.gene34002:CDS
CAGAAGATTCTACAACGCAA+AGG 0.606512 1.4:+74427041 MS.gene34002:CDS
TCGGTTCGTGAATTAGCCAA+AGG 0.607239 1.4:+74427248 MS.gene34002:CDS
TATGATGCCGATTGTTCCTG+GGG 0.607823 1.4:+74427610 MS.gene34002:CDS
CGTGGCTATTCATGGAGGCA+AGG 0.609440 1.4:+74428243 MS.gene34002:CDS
TTTGGATCTGGAGGTTACCG+AGG 0.611360 1.4:+74428715 MS.gene34002:CDS
ATCCTTCTTATATGCAAAGG+AGG 0.611900 1.4:-74427308 None:intergenic
GTATCTTCTCTTGAAAGGAG+TGG 0.613245 1.4:+74428216 MS.gene34002:CDS
TGATTCGGAGAAACTTAAAG+TGG 0.617162 1.4:+74427091 MS.gene34002:CDS
ACCAGCTCAGCCAAAACAGG+GGG 0.618612 1.4:-74428544 None:intergenic
CCAACGCCTATTCAGGTGCA+GGG 0.626028 1.4:+74427476 MS.gene34002:CDS
GATTATGATGGCAATGCAGG+AGG 0.626164 1.4:+74427583 MS.gene34002:CDS
CTTGCCTCCATGAATAGCCA+CGG 0.627088 1.4:-74428242 None:intergenic
AAATCTTGATGTTATTCAGG+AGG 0.630055 1.4:+74428078 MS.gene34002:CDS
GGTAGAGTATGTCAAGCAAG+AGG 0.633475 1.4:+74428099 MS.gene34002:CDS
ATTAGAACATCAGAAGAGTG+TGG 0.644869 1.4:+74428666 MS.gene34002:CDS
GGATAAGAAAACCCTTATGT+CGG 0.648731 1.4:+74427229 MS.gene34002:CDS
TCACAAAATATAAGAACAGG+GGG 0.651713 1.4:-74428163 None:intergenic
TCTCACAAAATATAAGAACA+GGG 0.655996 1.4:-74428165 None:intergenic
CTGAGCCTTTGCCTACTGGG+TGG 0.663961 1.4:+74427282 MS.gene34002:CDS
TGAAGATGTTGAAAACCAAG+GGG 0.665813 1.4:+74427444 MS.gene34002:CDS
AAAGGAGGCTTCCACCCAGT+AGG 0.671260 1.4:-74427293 None:intergenic
CTCACAAAATATAAGAACAG+GGG 0.671503 1.4:-74428164 None:intergenic
CTTGAAAGGAGTGGAAGCCG+TGG 0.671745 1.4:+74428225 MS.gene34002:CDS
ATTCCACCGATACAAAGCAA+AGG 0.674412 1.4:-74427746 None:intergenic
AACCAGCTCAGCCAAAACAG+GGG 0.687019 1.4:-74428545 None:intergenic
TAGTTGTTGCAACTCCCGGG+AGG 0.714712 1.4:+74427813 MS.gene34002:CDS
GAAGCCGTGGCTATTCATGG+AGG 0.745575 1.4:+74428238 MS.gene34002:CDS
CCAGCTCAGCCAAAACAGGG+GGG 0.756991 1.4:-74428543 None:intergenic
TTATGAGGAGTATATCCCTG+TGG 0.764837 1.4:+74426995 MS.gene34002:CDS
TGGATCTGGAGGTTACCGAG+GGG 0.774006 1.4:+74428717 MS.gene34002:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATCCTTAAAATTCTTAAT+CGG - chr1.4:74427404-74427423 None:intergenic 15.0%
!!! AATAATAGAAAGGATTATTT+TGG + chr1.4:74428697-74428716 MS.gene34002:CDS 15.0%
!! AAATTGAAAATTATGTCCAT+AGG + chr1.4:74428401-74428420 MS.gene34002:CDS 20.0%
!! TATCCTTAAAATTCTTAATC+GGG - chr1.4:74427403-74427422 None:intergenic 20.0%
!!! TCTTATATTTTGTGAGAATA+AGG + chr1.4:74428171-74428190 MS.gene34002:CDS 20.0%
!!! TTAAGAATTTTAAGGATATG+AGG + chr1.4:74427405-74427424 MS.gene34002:CDS 20.0%
! AAAACCTATTATTGTCAATG+TGG + chr1.4:74428039-74428058 MS.gene34002:CDS 25.0%
! AAACCTATTATTGTCAATGT+GGG + chr1.4:74428040-74428059 MS.gene34002:CDS 25.0%
! ATCCTTAAAATTCTTAATCG+GGG - chr1.4:74427402-74427421 None:intergenic 25.0%
! ATCCTTTCTATTATTTGCAA+TGG - chr1.4:74428692-74428711 None:intergenic 25.0%
! ATTTCTTCATTATCTTCCAT+TGG - chr1.4:74428574-74428593 None:intergenic 25.0%
! TCTCACAAAATATAAGAACA+GGG - chr1.4:74428168-74428187 None:intergenic 25.0%
! TTCTCACAAAATATAAGAAC+AGG - chr1.4:74428169-74428188 None:intergenic 25.0%
!! AAATGAATCTTGATAACTGT+AGG + chr1.4:74427861-74427880 MS.gene34002:CDS 25.0%
!! ACATAATTTTCAATTTCAGC+TGG - chr1.4:74428397-74428416 None:intergenic 25.0%
!! AGAATTTTTGTTACCTTTGA+AGG + chr1.4:74427700-74427719 MS.gene34002:CDS 25.0%
!! ATCAAGATTCATTTTCTTCT+TGG - chr1.4:74427855-74427874 None:intergenic 25.0%
!! GTTTTCAACATCTTCAAAAT+CGG - chr1.4:74427440-74427459 None:intergenic 25.0%
!!! TATGATTGGAATTGCTTTTA+CGG + chr1.4:74427526-74427545 MS.gene34002:CDS 25.0%
AAATCTTGATGTTATTCAGG+AGG + chr1.4:74428078-74428097 MS.gene34002:CDS 30.0%
ATCAGGAAAATCTAAACCTT+TGG - chr1.4:74428347-74428366 None:intergenic 30.0%
CAGTGGCATATTATTGTTAA+TGG + chr1.4:74427362-74427381 MS.gene34002:CDS 30.0%
CTCACAAAATATAAGAACAG+GGG - chr1.4:74428167-74428186 None:intergenic 30.0%
CTCTTCTGATGTTCTAATTT+AGG - chr1.4:74428664-74428683 None:intergenic 30.0%
GAAAATTATGTCCATAGGAT+TGG + chr1.4:74428406-74428425 MS.gene34002:CDS 30.0%
GAAGATAATGAAGAAATCAC+AGG + chr1.4:74428577-74428596 MS.gene34002:CDS 30.0%
TAGGTATTTAACGTTAGATG+AGG + chr1.4:74427880-74427899 MS.gene34002:CDS 30.0%
TCACAAAATATAAGAACAGG+GGG - chr1.4:74428166-74428185 None:intergenic 30.0%
TCCTTAAAATTCTTAATCGG+GGG - chr1.4:74427401-74427420 None:intergenic 30.0%
TCTGATTATTTGTCCATCAA+GGG + chr1.4:74427646-74427665 MS.gene34002:CDS 30.0%
TTGAAGATGTTGAAAACCAA+GGG + chr1.4:74427443-74427462 MS.gene34002:CDS 30.0%
TTTGAAGATGTTGAAAACCA+AGG + chr1.4:74427442-74427461 MS.gene34002:CDS 30.0%
! GTATGCCATTTCATCATTTA+AGG + chr1.4:74428282-74428301 MS.gene34002:CDS 30.0%
! GTTCTAATTTAGGACAATCA+CGG - chr1.4:74428654-74428673 None:intergenic 30.0%
! TTGATGACATGTTGAATATC+AGG - chr1.4:74428364-74428383 None:intergenic 30.0%
!! ATGATTGGAATTGCTTTTAC+GGG + chr1.4:74427527-74427546 MS.gene34002:CDS 30.0%
!! GTCTTCCAGTTATTTTATCT+GGG + chr1.4:74427498-74427517 MS.gene34002:CDS 30.0%
!! TTTTAAGGATATGAGGTTTC+CGG + chr1.4:74427412-74427431 MS.gene34002:CDS 30.0%
!!! AGAAAGGATTATTTTGGATC+TGG + chr1.4:74428703-74428722 MS.gene34002:CDS 30.0%
!!! TCTAGCAAAGTTCTGAATTT+TGG - chr1.4:74428008-74428027 None:intergenic 30.0%
AACATCTTCAAAATCGGATC+CGG - chr1.4:74427434-74427453 None:intergenic 35.0%
ACAGATTGTTCAACAAGAGA+AGG + chr1.4:74427187-74427206 MS.gene34002:CDS 35.0%
AGAAGATTCTACAACGCAAA+GGG + chr1.4:74427042-74427061 MS.gene34002:CDS 35.0%
ATCCTTCTTATATGCAAAGG+AGG - chr1.4:74427311-74427330 None:intergenic 35.0%
ATTAGAACATCAGAAGAGTG+TGG + chr1.4:74428666-74428685 MS.gene34002:CDS 35.0%
CATGAGTATCTTCTCTTGAA+AGG + chr1.4:74428211-74428230 MS.gene34002:CDS 35.0%
CTCAGTATAAGTAATCCCTT+TGG - chr1.4:74427267-74427286 None:intergenic 35.0%
GACATCCTTCTTATATGCAA+AGG - chr1.4:74427314-74427333 None:intergenic 35.0%
GGAAGAAGAAGATGATTATG+AGG + chr1.4:74426980-74426999 MS.gene34002:CDS 35.0%
GGACAAATAATCAGACCAAA+CGG - chr1.4:74427641-74427660 None:intergenic 35.0%
GGAGATGATTGAGAATTTGT+CGG + chr1.4:74427208-74427227 MS.gene34002:CDS 35.0%
GGATAAGAAAACCCTTATGT+CGG + chr1.4:74427229-74427248 MS.gene34002:CDS 35.0%
GGCAAATCTTGATGTTATTC+AGG + chr1.4:74428075-74428094 MS.gene34002:CDS 35.0%
GGCCATTGCAAATAATAGAA+AGG + chr1.4:74428687-74428706 MS.gene34002:CDS 35.0%
GTCTGATTATTTGTCCATCA+AGG + chr1.4:74427645-74427664 MS.gene34002:CDS 35.0%
GTGATTTGATTCAGAAACAG+TGG + chr1.4:74427345-74427364 MS.gene34002:CDS 35.0%
TAATTCACGAACCGACATAA+GGG - chr1.4:74427243-74427262 None:intergenic 35.0%
TATAAGAAGGATGTCGAAGA+AGG + chr1.4:74427319-74427338 MS.gene34002:CDS 35.0%
TATTTGTCCATCAAGGGAAT+TGG + chr1.4:74427652-74427671 MS.gene34002:CDS 35.0%
TCCAGCCTTAAATGATGAAA+TGG - chr1.4:74428290-74428309 None:intergenic 35.0%
TGAAGATGTTGAAAACCAAG+GGG + chr1.4:74427444-74427463 MS.gene34002:CDS 35.0%
TGCATTGCCATCATAATCAT+CGG - chr1.4:74427581-74427600 None:intergenic 35.0%
TTAAAATTCTTAATCGGGGG+CGG - chr1.4:74427398-74427417 None:intergenic 35.0%
TTGATCACTTCAAAGCTCAA+AGG + chr1.4:74427951-74427970 MS.gene34002:CDS 35.0%
TTGGCATAGTAGCAGAAAAT+AGG - chr1.4:74427985-74428004 None:intergenic 35.0%
! TAAGCCAAGTCTACTTGTTA+AGG + chr1.4:74427121-74427140 MS.gene34002:CDS 35.0%
! TCTGTTTAGCTTCTTGCAAA+AGG - chr1.4:74428519-74428538 None:intergenic 35.0%
! TGATTCGGAGAAACTTAAAG+TGG + chr1.4:74427091-74427110 MS.gene34002:CDS 35.0%
! TTTTGCAAGAAGCTAAACAG+AGG + chr1.4:74428518-74428537 MS.gene34002:CDS 35.0%
!! GGTCTTCCAGTTATTTTATC+TGG + chr1.4:74427497-74427516 MS.gene34002:CDS 35.0%
!! TCCAGTTATTTTATCTGGGA+GGG + chr1.4:74427502-74427521 MS.gene34002:CDS 35.0%
!! TTCCAGTTATTTTATCTGGG+AGG + chr1.4:74427501-74427520 MS.gene34002:CDS 35.0%
!!! AAGGATTATTTTGGATCTGG+AGG + chr1.4:74428706-74428725 MS.gene34002:CDS 35.0%
!!! GCAAAGTTCTGAATTTTGGT+TGG - chr1.4:74428004-74428023 None:intergenic 35.0%
ACAGCTTGAGATTGTCAAGA+AGG + chr1.4:74427781-74427800 MS.gene34002:CDS 40.0%
AGCCTCCTTTGCATATAAGA+AGG + chr1.4:74427306-74427325 MS.gene34002:CDS 40.0%
ATATAGTTGTTGCAACTCCC+GGG + chr1.4:74427810-74427829 MS.gene34002:CDS 40.0%
ATTCCACCGATACAAAGCAA+AGG - chr1.4:74427749-74427768 None:intergenic 40.0%
CAAAATATAAGAACAGGGGG+TGG - chr1.4:74428163-74428182 None:intergenic 40.0%
CAAGCAATGTTGTCTCACTT+TGG - chr1.4:74428486-74428505 None:intergenic 40.0%
CAGAAGATTCTACAACGCAA+AGG + chr1.4:74427041-74427060 MS.gene34002:CDS 40.0%
CAGCTTGAGATTGTCAAGAA+GGG + chr1.4:74427782-74427801 MS.gene34002:CDS 40.0%
CATATAGTTGTTGCAACTCC+CGG + chr1.4:74427809-74427828 MS.gene34002:CDS 40.0%
CGAATCATCTTCTTCCATTG+CGG - chr1.4:74427078-74427097 None:intergenic 40.0%
CGCAATGGAAGAAGATGATT+CGG + chr1.4:74427076-74427095 MS.gene34002:CDS 40.0%
CGTCCCACATTGACAATAAT+AGG - chr1.4:74428046-74428065 None:intergenic 40.0%
CTAATTCACGAACCGACATA+AGG - chr1.4:74427244-74427263 None:intergenic 40.0%
GAAGAAATCACAGGCATAAG+TGG + chr1.4:74428586-74428605 MS.gene34002:CDS 40.0%
GATGATTATGATGGCAATGC+AGG + chr1.4:74427580-74427599 MS.gene34002:CDS 40.0%
GATGCCTTAACAAGTAGACT+TGG - chr1.4:74427128-74427147 None:intergenic 40.0%
GTATCTTCTCTTGAAAGGAG+TGG + chr1.4:74428216-74428235 MS.gene34002:CDS 40.0%
TAGAATCTTCTGAGCTTCCA+TGG - chr1.4:74427033-74427052 None:intergenic 40.0%
TCCCTCCCAGATAAAATAAC+TGG - chr1.4:74427506-74427525 None:intergenic 40.0%
TGTGCTTCCGATGATTATGA+TGG + chr1.4:74427571-74427590 MS.gene34002:CDS 40.0%
TTATCTGGGAGGGATATGAT+TGG + chr1.4:74427512-74427531 MS.gene34002:CDS 40.0%
TTTGAAGGAAGCTGGATATC+CGG + chr1.4:74427715-74427734 MS.gene34002:CDS 40.0%
! ATTATGATGCCGATTGTTCC+TGG + chr1.4:74427608-74427627 MS.gene34002:CDS 40.0%
! GCCATTTCATCATTTAAGGC+TGG + chr1.4:74428286-74428305 MS.gene34002:CDS 40.0%
! GCCCCCGATTAAGAATTTTA+AGG + chr1.4:74427397-74427416 MS.gene34002:CDS 40.0%
! TACGGGTTCTGGTAAAACTT+TGG + chr1.4:74427544-74427563 MS.gene34002:CDS 40.0%
! TTATGAGGAGTATATCCCTG+TGG + chr1.4:74426995-74427014 MS.gene34002:CDS 40.0%
! TTATGATGCCGATTGTTCCT+GGG + chr1.4:74427609-74427628 MS.gene34002:CDS 40.0%
! TTGTTACCTTTGAAGGAAGC+TGG + chr1.4:74427707-74427726 MS.gene34002:CDS 40.0%
!! GAACTTTGCTAGAAGTGCTT+TGG + chr1.4:74428015-74428034 MS.gene34002:CDS 40.0%
AACATATCCTTCAACCTCCC+GGG - chr1.4:74427830-74427849 None:intergenic 45.0%
ATACTGAGCCTTTGCCTACT+GGG + chr1.4:74427279-74427298 MS.gene34002:CDS 45.0%
ATCATCTTCTTCCATTGCGG+CGG - chr1.4:74427075-74427094 None:intergenic 45.0%
CAACATATCCTTCAACCTCC+CGG - chr1.4:74427831-74427850 None:intergenic 45.0%
CGGTTCGTGAATTAGCCAAA+GGG + chr1.4:74427249-74427268 MS.gene34002:CDS 45.0%
CTCAGGCCTTTGCTTTGTAT+CGG + chr1.4:74427740-74427759 MS.gene34002:CDS 45.0%
GATTATGATGGCAATGCAGG+AGG + chr1.4:74427583-74427602 MS.gene34002:CDS 45.0%
GCAACTGATGTTGCATCCAA+AGG + chr1.4:74428328-74428347 MS.gene34002:CDS 45.0%
GGATATCCAGCTTCCTTCAA+AGG - chr1.4:74427716-74427735 None:intergenic 45.0%
GGCTGGAAAGAAAGATGTGT+TGG + chr1.4:74428303-74428322 MS.gene34002:CDS 45.0%
GGTAGAGTATGTCAAGCAAG+AGG + chr1.4:74428099-74428118 MS.gene34002:CDS 45.0%
GTTAGATGAGGCTGATCGAT+TGG + chr1.4:74427892-74427911 MS.gene34002:CDS 45.0%
TATACTGAGCCTTTGCCTAC+TGG + chr1.4:74427278-74427297 MS.gene34002:CDS 45.0%
TATTCATGGAGGCAAGGATC+AGG + chr1.4:74428249-74428268 MS.gene34002:CDS 45.0%
TGTTCTGTGACACTGATCTC+AGG - chr1.4:74427170-74427189 None:intergenic 45.0%
! ACAGGCATAAGTGGTGTCAA+GGG + chr1.4:74428595-74428614 MS.gene34002:CDS 45.0%
! ACCAGTTTTGCCACATCTTC+CGG - chr1.4:74428437-74428456 None:intergenic 45.0%
! GCTGATCGATTGGTCGATTT+GGG + chr1.4:74427902-74427921 MS.gene34002:CDS 45.0%
! TATGATGCCGATTGTTCCTG+GGG + chr1.4:74427610-74427629 MS.gene34002:CDS 45.0%
! TCGGTTCGTGAATTAGCCAA+AGG + chr1.4:74427248-74427267 MS.gene34002:CDS 45.0%
!! GGAATTGCTTTTACGGGTTC+TGG + chr1.4:74427533-74427552 MS.gene34002:CDS 45.0%
!!! GAGTTTTCTGAAGGCACTCA+AGG - chr1.4:74428141-74428160 None:intergenic 45.0%
!!! TTTTCTGAAGGCACTCAAGG+AGG - chr1.4:74428138-74428157 None:intergenic 45.0%
AACCAGCTCAGCCAAAACAG+GGG - chr1.4:74428548-74428567 None:intergenic 50.0%
AAGGGCTGTGCATATTGTGG+CGG + chr1.4:74428613-74428632 MS.gene34002:CDS 50.0%
ACCGGAAGATGTGGCAAAAC+TGG + chr1.4:74428433-74428452 MS.gene34002:CDS 50.0%
AGGCCTTTGCTTTGTATCGG+TGG + chr1.4:74427743-74427762 MS.gene34002:CDS 50.0%
ATTGGACGTACCGGAAGATG+TGG + chr1.4:74428424-74428443 MS.gene34002:CDS 50.0%
ATTGTCAATGTGGGACGTGC+AGG + chr1.4:74428049-74428068 MS.gene34002:CDS 50.0%
CAACCAGCTCAGCCAAAACA+GGG - chr1.4:74428549-74428568 None:intergenic 50.0%
CGGGAGGTTGAAGGATATGT+TGG + chr1.4:74427829-74427848 MS.gene34002:CDS 50.0%
CTTGCCTCCATGAATAGCCA+CGG - chr1.4:74428245-74428264 None:intergenic 50.0%
GTCAAGGGCTGTGCATATTG+TGG + chr1.4:74428610-74428629 MS.gene34002:CDS 50.0%
GTCCATAGGATTGGACGTAC+CGG + chr1.4:74428415-74428434 MS.gene34002:CDS 50.0%
GTCCATCAAGGGAATTGGCT+AGG + chr1.4:74427657-74427676 MS.gene34002:CDS 50.0%
TCAACCAGCTCAGCCAAAAC+AGG - chr1.4:74428550-74428569 None:intergenic 50.0%
TGCCTAGCCAATTCCCTTGA+TGG - chr1.4:74427662-74427681 None:intergenic 50.0%
TTCCGGTACGTCCAATCCTA+TGG - chr1.4:74428420-74428439 None:intergenic 50.0%
TTGGATCTGGAGGTTACCGA+GGG + chr1.4:74428716-74428735 MS.gene34002:CDS 50.0%
TTGTCAATGTGGGACGTGCA+GGG + chr1.4:74428050-74428069 MS.gene34002:CDS 50.0%
TTTGGATCTGGAGGTTACCG+AGG + chr1.4:74428715-74428734 MS.gene34002:CDS 50.0%
! CACAGGCATAAGTGGTGTCA+AGG + chr1.4:74428594-74428613 MS.gene34002:CDS 50.0%
! GGCTGATCGATTGGTCGATT+TGG + chr1.4:74427901-74427920 MS.gene34002:CDS 50.0%
AAAGGAGGCTTCCACCCAGT+AGG - chr1.4:74427296-74427315 None:intergenic 55.0%
AAGCTGGATATCCGGAGCTC+AGG + chr1.4:74427723-74427742 MS.gene34002:CDS 55.0%
ACCAGCTCAGCCAAAACAGG+GGG - chr1.4:74428547-74428566 None:intergenic 55.0%
AGGGCTGTGCATATTGTGGC+GGG + chr1.4:74428614-74428633 MS.gene34002:CDS 55.0%
CAAAGCAAAGGCCTGAGCTC+CGG - chr1.4:74427737-74427756 None:intergenic 55.0%
CCAACGCCTATTCAGGTGCA+GGG + chr1.4:74427476-74427495 MS.gene34002:CDS 55.0%
CGTGGCTATTCATGGAGGCA+AGG + chr1.4:74428243-74428262 MS.gene34002:CDS 55.0%
CTTGAAAGGAGTGGAAGCCG+TGG + chr1.4:74428225-74428244 MS.gene34002:CDS 55.0%
GAAGCCGTGGCTATTCATGG+AGG + chr1.4:74428238-74428257 MS.gene34002:CDS 55.0%
GGAAGACCCTGCACCTGAAT+AGG - chr1.4:74427485-74427504 None:intergenic 55.0%
GGACCTTCCCCAGGAACAAT+CGG - chr1.4:74427620-74427639 None:intergenic 55.0%
GGCTGAGCTGGTTGATCCAA+TGG + chr1.4:74428555-74428574 MS.gene34002:CDS 55.0%
GTGGAAGCCGTGGCTATTCA+TGG + chr1.4:74428235-74428254 MS.gene34002:CDS 55.0%
TAGTTGTTGCAACTCCCGGG+AGG + chr1.4:74427813-74427832 MS.gene34002:CDS 55.0%
TGGATCTGGAGGTTACCGAG+GGG + chr1.4:74428717-74428736 MS.gene34002:CDS 55.0%
TGTCAATGTGGGACGTGCAG+GGG + chr1.4:74428051-74428070 MS.gene34002:CDS 55.0%
TGTGCAGCCAACGCCTATTC+AGG + chr1.4:74427469-74427488 MS.gene34002:CDS 55.0%
! ACAGAGGATCCCCCCTGTTT+TGG + chr1.4:74428534-74428553 MS.gene34002:CDS 55.0%
! ATGCCGATTGTTCCTGGGGA+AGG + chr1.4:74427614-74427633 MS.gene34002:CDS 55.0%
! CAGGGGGTGGAGTTTTCTGA+AGG - chr1.4:74428150-74428169 None:intergenic 55.0%
!! TGTGCATATTGTGGCGGGCT+TGG + chr1.4:74428619-74428638 MS.gene34002:CDS 55.0%
AGACCAAACGGACCTTCCCC+AGG - chr1.4:74427629-74427648 None:intergenic 60.0%
AGGGAAAGCAACCGCCGCAA+TGG + chr1.4:74427061-74427080 MS.gene34002:CDS 60.0%
CCAGCTCAGCCAAAACAGGG+GGG - chr1.4:74428546-74428565 None:intergenic 60.0%
CCCTGCACCTGAATAGGCGT+TGG - chr1.4:74427479-74427498 None:intergenic 60.0%
CTGAGCCTTTGCCTACTGGG+TGG + chr1.4:74427282-74427301 MS.gene34002:CDS 60.0%
GCCAACGCCTATTCAGGTGC+AGG + chr1.4:74427475-74427494 MS.gene34002:CDS 60.0%
GGCTTCCACCCAGTAGGCAA+AGG - chr1.4:74427290-74427309 None:intergenic 60.0%
GTTCCTGGGGAAGGTCCGTT+TGG + chr1.4:74427623-74427642 MS.gene34002:CDS 60.0%
TGCAACTCCCGGGAGGTTGA+AGG + chr1.4:74427820-74427839 MS.gene34002:CDS 60.0%
! CGTTGGCTGCACAATCCCCT+TGG - chr1.4:74427462-74427481 None:intergenic 60.0%
AGCTTCCATGGCGCGACGCT+TGG - chr1.4:74427021-74427040 None:intergenic 65.0%
CAATGTGGGACGTGCAGGGG+CGG + chr1.4:74428054-74428073 MS.gene34002:CDS 65.0%
!!! CCCCCCTGTTTTGGCTGAGC+TGG + chr1.4:74428543-74428562 MS.gene34002:CDS 65.0%
ATGGCGCGACGCTTGGCCAC+AGG - chr1.4:74427014-74427033 None:intergenic 70.0%
TGGCGCGACGCTTGGCCACA+GGG - chr1.4:74427013-74427032 None:intergenic 70.0%
! TGTGGCCAAGCGTCGCGCCA+TGG + chr1.4:74427013-74427032 MS.gene34002:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 74426978 74428747 74426978 ID=MS.gene34002
chr1.4 mRNA 74426978 74428747 74426978 ID=MS.gene34002.t1;Parent=MS.gene34002
chr1.4 exon 74426978 74428747 74426978 ID=MS.gene34002.t1.exon1;Parent=MS.gene34002.t1
chr1.4 CDS 74426978 74428747 74426978 ID=cds.MS.gene34002.t1;Parent=MS.gene34002.t1
Gene Sequence

>MS.gene34002

ATGGAAGAAGAAGATGATTATGAGGAGTATATCCCTGTGGCCAAGCGTCGCGCCATGGAAGCTCAGAAGATTCTACAACGCAAAGGGAAAGCAACCGCCGCAATGGAAGAAGATGATTCGGAGAAACTTAAAGTGGTTGAAACTAAGCCAAGTCTACTTGTTAAGGCATCACAGTTGAAGAAAGATCAACCTGAGATCAGTGTCACAGAACAGATTGTTCAACAAGAGAAGGAGATGATTGAGAATTTGTCGGATAAGAAAACCCTTATGTCGGTTCGTGAATTAGCCAAAGGGATTACTTATACTGAGCCTTTGCCTACTGGGTGGAAGCCTCCTTTGCATATAAGAAGGATGTCGAAGAAGGATTGTGATTTGATTCAGAAACAGTGGCATATTATTGTTAATGGCGAAGAGATTCCGCCCCCGATTAAGAATTTTAAGGATATGAGGTTTCCGGATCCGATTTTGAAGATGTTGAAAACCAAGGGGATTGTGCAGCCAACGCCTATTCAGGTGCAGGGTCTTCCAGTTATTTTATCTGGGAGGGATATGATTGGAATTGCTTTTACGGGTTCTGGTAAAACTTTGGTTTTTGTGCTTCCGATGATTATGATGGCAATGCAGGAGGAGATTATGATGCCGATTGTTCCTGGGGAAGGTCCGTTTGGTCTGATTATTTGTCCATCAAGGGAATTGGCTAGGCAAACTTATGAAGTCATTGAAGAATTTTTGTTACCTTTGAAGGAAGCTGGATATCCGGAGCTCAGGCCTTTGCTTTGTATCGGTGGAATTGATATGAGATCACAGCTTGAGATTGTCAAGAAGGGTGTTCATATAGTTGTTGCAACTCCCGGGAGGTTGAAGGATATGTTGGCCAAGAAGAAAATGAATCTTGATAACTGTAGGTATTTAACGTTAGATGAGGCTGATCGATTGGTCGATTTGGGATTTGAAGATGATATAAGAGAAGTTTTTGATCACTTCAAAGCTCAAAGGCAAACTCTCCTATTTTCTGCTACTATGCCAACCAAAATTCAGAACTTTGCTAGAAGTGCTTTGGTAAAACCTATTATTGTCAATGTGGGACGTGCAGGGGCGGCAAATCTTGATGTTATTCAGGAGGTAGAGTATGTCAAGCAAGAGGCGAAAATAGTTTACCTCCTTGAGTGCCTTCAGAAAACTCCACCCCCTGTTCTTATATTTTGTGAGAATAAGGCTGATGTTGATGACATTCATGAGTATCTTCTCTTGAAAGGAGTGGAAGCCGTGGCTATTCATGGAGGCAAGGATCAGGAAGAGAGAGAGTATGCCATTTCATCATTTAAGGCTGGAAAGAAAGATGTGTTGGTTGCAACTGATGTTGCATCCAAAGGTTTAGATTTTCCTGATATTCAACATGTCATCAACTATGATATGCCAGCTGAAATTGAAAATTATGTCCATAGGATTGGACGTACCGGAAGATGTGGCAAAACTGGTATAGCAACAACATTTATAAACAAGAACCAAAGTGAGACAACATTGCTTGATTTGAAACACCTTTTGCAAGAAGCTAAACAGAGGATCCCCCCTGTTTTGGCTGAGCTGGTTGATCCAATGGAAGATAATGAAGAAATCACAGGCATAAGTGGTGTCAAGGGCTGTGCATATTGTGGCGGGCTTGGTCATCGTATCCGTGATTGTCCTAAATTAGAACATCAGAAGAGTGTGGCCATTGCAAATAATAGAAAGGATTATTTTGGATCTGGAGGTTACCGAGGGGAAATTTGA
Protein sequence

>MS.gene34002.t1

MEEEDDYEEYIPVAKRRAMEAQKILQRKGKATAAMEEDDSEKLKVVETKPSLLVKASQLKKDQPEISVTEQIVQQEKEMIENLSDKKTLMSVRELAKGITYTEPLPTGWKPPLHIRRMSKKDCDLIQKQWHIIVNGEEIPPPIKNFKDMRFPDPILKMLKTKGIVQPTPIQVQGLPVILSGRDMIGIAFTGSGKTLVFVLPMIMMAMQEEIMMPIVPGEGPFGLIICPSRELARQTYEVIEEFLLPLKEAGYPELRPLLCIGGIDMRSQLEIVKKGVHIVVATPGRLKDMLAKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPIIVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIHEYLLLKGVEAVAIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELVDPMEDNEEITGISGVKGCAYCGGLGHRIRDCPKLEHQKSVAIANNRKDYFGSGGYRGEI