AlfalfaGEDB | MS.gene34422

M. sativa cultivar XinJiangDaYe / MS.gene34422

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046983
PFAM: PF00010.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34422.t1	XP_003592388.2	95.5	245	10	1	1	245	1	244	1.50E-126	462.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34422.t1	Q9FLI1	45.3	172	84	2	76	237	2	173	3.9e-27	123.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34422.t1	G7ID49	95.5	245	10	1	1	245	1	244	1.1e-126	462.2

TFs/TRs:

Gene ID	Type	Classification
MS.gene34422.t1	TF	bHLH

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene34422	MS.gene37667	PPI
MS.gene070993	MS.gene34422	PPI
MS.gene34422	MS.gene34423	PPI
MS.gene34422	MS.gene006365	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34422.t1	MTR_1g102370	95.510	245	10	1	1	245	1	244	1.08e-172	475
MS.gene34422.t1	MTR_0007s0380	52.193	228	99	6	21	243	16	238	4.45e-65	202
MS.gene34422.t1	MTR_0007s0330	40.223	179	97	3	72	244	14	188	4.37e-34	122
MS.gene34422.t1	MTR_0007s0330	41.615	161	87	2	72	226	2	161	1.25e-32	117
MS.gene34422.t1	MTR_1g102380	35.714	182	101	2	61	231	4	180	3.95e-29	108

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34422.t1	AT5G51780	46.328	177	79	5	74	237	23	196	6.51e-38	131
MS.gene34422.t1	AT5G51780	46.286	175	78	5	76	237	2	173	1.65e-37	130
MS.gene34422.t1	AT4G25400	44.444	171	88	3	70	237	19	185	3.00e-36	127
MS.gene34422.t1	AT4G25400	46.405	153	75	3	70	219	19	167	1.30e-33	120
MS.gene34422.t1	AT4G25410	43.636	165	83	4	80	234	47	211	2.83e-33	120
MS.gene34422.t1	AT5G51790	40.541	185	98	5	62	234	9	193	6.73e-32	116
MS.gene34422.t1	AT5G51790	40.541	185	98	5	62	234	74	258	2.31e-31	116
MS.gene34422.t1	AT5G51790	41.765	170	87	5	62	219	9	178	2.36e-30	112
MS.gene34422.t1	AT5G51790	41.765	170	87	5	62	219	74	243	9.96e-30	112
MS.gene34422.t1	AT1G62975	32.161	199	111	3	63	237	61	259	3.06e-24	97.8
MS.gene34422.t1	AT1G12540	35.196	179	101	5	76	240	75	252	5.61e-22	91.7
MS.gene34422.t1	AT1G62975	33.149	181	97	3	63	219	61	241	7.29e-22	91.3

Find 42 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCCCCACTTTCTAAATTTC+TGG	0.103688	1.2:+76166139	MS.gene34422:CDS
GAAGAGGGAGAAGGGATCTA+AGG	0.225427	1.2:-76165795	None:intergenic
CTGGAACAACTGATGAAATA+TGG	0.304748	1.2:+76166158	MS.gene34422:CDS
TATTGAAGATAAGAAGAAAA+TGG	0.318828	1.2:+76165717	MS.gene34422:CDS
TCTATATAAAATAATTTGTT+TGG	0.336326	1.2:-76165664	None:intergenic
ATAAGAAGAAAATGGTTCAT+AGG	0.350975	1.2:+76165725	MS.gene34422:CDS
ACTAAATGATGGATTCATAT+TGG	0.351963	1.2:-76166398	None:intergenic
AAATCACTTGAAGAAGAATA+TGG	0.357120	1.2:+76165965	MS.gene34422:CDS
TATATAGATCGTACAAATCA+TGG	0.422086	1.2:+76165679	MS.gene34422:CDS
TACTAGTACTTGCTTTATAG+AGG	0.425326	1.2:+76166112	MS.gene34422:CDS
GTGGTTGAGACTATTAGATA+AGG	0.433605	1.2:-76165542	None:intergenic
TGGAAGAACTAAGTTACAAA+AGG	0.434554	1.2:+76165985	MS.gene34422:CDS
ATACCTCACACTGCACACTA+TGG	0.439476	1.2:-76166229	None:intergenic
CCACAGTTCTATTATTCTTG+TGG	0.455790	1.2:-76166076	None:intergenic
ATATAAAATAATTTGTTTGG+TGG	0.460635	1.2:-76165661	None:intergenic
AAGAGGGAGAAGGGATCTAA+GGG	0.467872	1.2:-76165794	None:intergenic
TAATCTGAACAACTAAATGA+TGG	0.468144	1.2:-76166409	None:intergenic
TAAGAAGAAAATGGTTCATA+GGG	0.475156	1.2:+76165726	MS.gene34422:CDS
TAGATCTAACTGAGCTAAGA+AGG	0.478104	1.2:+76166368	MS.gene34422:CDS
TTTACAACGATGCAATGAAC+TGG	0.483997	1.2:+76165513	MS.gene34422:CDS
AGTTCATTGCATCGTTGTAA+AGG	0.484172	1.2:-76165511	None:intergenic
AAAATAATTTGTTTGGTGGC+CGG	0.487549	1.2:-76165657	None:intergenic
TGGTTGAGACTATTAGATAA+GGG	0.494385	1.2:-76165541	None:intergenic
GTTCCAGAAATTTAGAAAGT+GGG	0.518377	1.2:-76166142	None:intergenic
TTTATCTGATCAAATGAATG+AGG	0.525114	1.2:+76165929	MS.gene34422:CDS
TGTTCCAGAAATTTAGAAAG+TGG	0.525663	1.2:-76166143	None:intergenic
CACAAGAATAATAGAACTGT+GGG	0.526811	1.2:+76166077	MS.gene34422:CDS
GGAAGAACTAAGTTACAAAA+GGG	0.531740	1.2:+76165986	MS.gene34422:CDS
GAAGCAAAGGAGACAAGAAA+TGG	0.533586	1.2:+76165756	MS.gene34422:CDS
TCTCCCTCTTCACTTCATCA+AGG	0.533778	1.2:+76165807	MS.gene34422:CDS
TCACCTTGATGAAGTGAAGA+GGG	0.538188	1.2:-76165810	None:intergenic
GATGAAGTGAAGAGGGAGAA+GGG	0.546212	1.2:-76165803	None:intergenic
CCCACAACATCACAAAATCT+CGG	0.553306	1.2:+76165567	MS.gene34422:CDS
AGTACTTGCTTTATAGAGGA+AGG	0.558027	1.2:+76166116	MS.gene34422:CDS
TTCCAGAAATTTAGAAAGTG+GGG	0.570258	1.2:-76166141	None:intergenic
ATAGGGAAATTGAGAAGCAA+AGG	0.575199	1.2:+76165743	MS.gene34422:CDS
TGATGAAGTGAAGAGGGAGA+AGG	0.577558	1.2:-76165804	None:intergenic
CTCAGTTAGATCTACACTGT+CGG	0.580625	1.2:-76166359	None:intergenic
TTCACCTTGATGAAGTGAAG+AGG	0.603402	1.2:-76165811	None:intergenic
CCACAAGAATAATAGAACTG+TGG	0.617517	1.2:+76166076	MS.gene34422:CDS
ACTCCATAGTGTGCAGTGTG+AGG	0.655288	1.2:+76166226	MS.gene34422:CDS
TAGTGAAACTACAAGAAACA+TGG	0.661078	1.2:-76166052	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TCTATATAAAATAATTTGTT+TGG	-	chr1.2:76165667-76165686	None:intergenic	10.0%
!!!	ATATAAAATAATTTGTTTGG+TGG	-	chr1.2:76165664-76165683	None:intergenic	15.0%
!!!	TGATTTTTCTCTTAAAAAAA+TGG	+	chr1.2:76165627-76165646	MS.gene34422:CDS	15.0%
!!!	TTTAATCATTAAAGTTTTCT+AGG	+	chr1.2:76165849-76165868	MS.gene34422:intron	15.0%
!!	TATCATCTGTTATAACTTTA+GGG	+	chr1.2:76165873-76165892	MS.gene34422:intron	20.0%
!!	TATTGAAGATAAGAAGAAAA+TGG	+	chr1.2:76165717-76165736	MS.gene34422:CDS	20.0%
!!	TTAAAAAAATGGATACTAAC+CGG	+	chr1.2:76165638-76165657	MS.gene34422:CDS	20.0%
!!	TTATCATCTGTTATAACTTT+AGG	+	chr1.2:76165872-76165891	MS.gene34422:intron	20.0%
!!!	AAACATGGCTTTTATTTTTA+AGG	-	chr1.2:76166040-76166059	None:intergenic	20.0%
!!!	TATTTATTGTTTCTCTAACA+GGG	+	chr1.2:76165897-76165916	MS.gene34422:intron	20.0%
!!!	TTATTTATTGTTTCTCTAAC+AGG	+	chr1.2:76165896-76165915	MS.gene34422:intron	20.0%
!	AAATCACTTGAAGAAGAATA+TGG	+	chr1.2:76165965-76165984	MS.gene34422:CDS	25.0%
!	ATAAGAAGAAAATGGTTCAT+AGG	+	chr1.2:76165725-76165744	MS.gene34422:CDS	25.0%
!	TAAGAAGAAAATGGTTCATA+GGG	+	chr1.2:76165726-76165745	MS.gene34422:CDS	25.0%
!	TAATCTGAACAACTAAATGA+TGG	-	chr1.2:76166412-76166431	None:intergenic	25.0%
!	TATATAGATCGTACAAATCA+TGG	+	chr1.2:76165679-76165698	MS.gene34422:CDS	25.0%
!	TTTATCTGATCAAATGAATG+AGG	+	chr1.2:76165929-76165948	MS.gene34422:CDS	25.0%
!!!	ACTAAATGATGGATTCATAT+TGG	-	chr1.2:76166401-76166420	None:intergenic	25.0%
	CACAAGAATAATAGAACTGT+GGG	+	chr1.2:76166077-76166096	MS.gene34422:CDS	30.0%
	GGAAGAACTAAGTTACAAAA+GGG	+	chr1.2:76165986-76166005	MS.gene34422:CDS	30.0%
	GTTCCAGAAATTTAGAAAGT+GGG	-	chr1.2:76166145-76166164	None:intergenic	30.0%
	TACTAGTACTTGCTTTATAG+AGG	+	chr1.2:76166112-76166131	MS.gene34422:CDS	30.0%
	TAGTGAAACTACAAGAAACA+TGG	-	chr1.2:76166055-76166074	None:intergenic	30.0%
	TGGAAGAACTAAGTTACAAA+AGG	+	chr1.2:76165985-76166004	MS.gene34422:CDS	30.0%
	TGGTTGAGACTATTAGATAA+GGG	-	chr1.2:76165544-76165563	None:intergenic	30.0%
	TGTTCCAGAAATTTAGAAAG+TGG	-	chr1.2:76166146-76166165	None:intergenic	30.0%
	TTCCAGAAATTTAGAAAGTG+GGG	-	chr1.2:76166144-76166163	None:intergenic	30.0%
!	TGCTTTTCTATTCAAGTCAA+TGG	+	chr1.2:76166197-76166216	MS.gene34422:CDS	30.0%
!!	AAAATAATTTGTTTGGTGGC+CGG	-	chr1.2:76165660-76165679	None:intergenic	30.0%
!!!	TGGCTTTTATTTTTAAGGCT+AGG	-	chr1.2:76166035-76166054	None:intergenic	30.0%
	AGTACTTGCTTTATAGAGGA+AGG	+	chr1.2:76166116-76166135	MS.gene34422:CDS	35.0%
	AGTTCATTGCATCGTTGTAA+AGG	-	chr1.2:76165514-76165533	None:intergenic	35.0%
	ATAGGGAAATTGAGAAGCAA+AGG	+	chr1.2:76165743-76165762	MS.gene34422:CDS	35.0%
	CCACAAGAATAATAGAACTG+TGG	+	chr1.2:76166076-76166095	MS.gene34422:CDS	35.0%
	CCACAGTTCTATTATTCTTG+TGG	-	chr1.2:76166079-76166098	None:intergenic	35.0%
	CTGGAACAACTGATGAAATA+TGG	+	chr1.2:76166158-76166177	MS.gene34422:CDS	35.0%
	GTGGTTGAGACTATTAGATA+AGG	-	chr1.2:76165545-76165564	None:intergenic	35.0%
	TAGATCTAACTGAGCTAAGA+AGG	+	chr1.2:76166368-76166387	MS.gene34422:CDS	35.0%
	TTTACAACGATGCAATGAAC+TGG	+	chr1.2:76165513-76165532	MS.gene34422:CDS	35.0%
!	TATTGAGCTTGATGATTCAC+AGG	+	chr1.2:76166327-76166346	MS.gene34422:intron	35.0%
	CCCACAACATCACAAAATCT+CGG	+	chr1.2:76165567-76165586	MS.gene34422:CDS	40.0%
	CTCAGTTAGATCTACACTGT+CGG	-	chr1.2:76166362-76166381	None:intergenic	40.0%
	GAAGCAAAGGAGACAAGAAA+TGG	+	chr1.2:76165756-76165775	MS.gene34422:CDS	40.0%
	TCACCTTGATGAAGTGAAGA+GGG	-	chr1.2:76165813-76165832	None:intergenic	40.0%
	TGCCCCACTTTCTAAATTTC+TGG	+	chr1.2:76166139-76166158	MS.gene34422:CDS	40.0%
	TTCACCTTGATGAAGTGAAG+AGG	-	chr1.2:76165814-76165833	None:intergenic	40.0%
!	AGATTTTGTGATGTTGTGGG+TGG	-	chr1.2:76165567-76165586	None:intergenic	40.0%
!	CCGAGATTTTGTGATGTTGT+GGG	-	chr1.2:76165570-76165589	None:intergenic	40.0%
!	TCCGAGATTTTGTGATGTTG+TGG	-	chr1.2:76165571-76165590	None:intergenic	40.0%
	AAGAGGGAGAAGGGATCTAA+GGG	-	chr1.2:76165797-76165816	None:intergenic	45.0%
	ATACCTCACACTGCACACTA+TGG	-	chr1.2:76166232-76166251	None:intergenic	45.0%
	GATGAAGTGAAGAGGGAGAA+GGG	-	chr1.2:76165806-76165825	None:intergenic	45.0%
	TCTCCCTCTTCACTTCATCA+AGG	+	chr1.2:76165807-76165826	MS.gene34422:CDS	45.0%
	TGATGAAGTGAAGAGGGAGA+AGG	-	chr1.2:76165807-76165826	None:intergenic	45.0%
!	TTTTGTGATGTTGTGGGTGG+TGG	-	chr1.2:76165564-76165583	None:intergenic	45.0%
	ACTCCATAGTGTGCAGTGTG+AGG	+	chr1.2:76166226-76166245	MS.gene34422:CDS	50.0%
	GAAGAGGGAGAAGGGATCTA+AGG	-	chr1.2:76165798-76165817	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	76165505	76166432	76165505	ID=MS.gene34422
chr1.2	mRNA	76165505	76166432	76165505	ID=MS.gene34422.t1;Parent=MS.gene34422
chr1.2	exon	76165505	76165828	76165505	ID=MS.gene34422.t1.exon1;Parent=MS.gene34422.t1
chr1.2	CDS	76165505	76165828	76165505	ID=cds.MS.gene34422.t1;Parent=MS.gene34422.t1
chr1.2	exon	76165918	76166247	76165918	ID=MS.gene34422.t1.exon2;Parent=MS.gene34422.t1
chr1.2	CDS	76165918	76166247	76165918	ID=cds.MS.gene34422.t1;Parent=MS.gene34422.t1
chr1.2	exon	76166349	76166432	76166349	ID=MS.gene34422.t1.exon3;Parent=MS.gene34422.t1
chr1.2	CDS	76166349	76166432	76166349	ID=cds.MS.gene34422.t1;Parent=MS.gene34422.t1

Gene Sequence

>MS.gene34422

ATGTTTCCTTTACAACGATGCAATGAACTGGCGAAACCCTTATCTAATAGTCTCAACCACCACCCACAACATCACAAAATCTCGGAAGATCTGATTCTTGATGATTGTGATTCTCTTGTAATTGATTTTTCTCTTAAAAAAATGGATACTAACCGGCCACCAAACAAATTATTTTATATAGATCGTACAAATCATGGTAGTAATCGTAATTCTATTGAAGATAAGAAGAAAATGGTTCATAGGGAAATTGAGAAGCAAAGGAGACAAGAAATGGCTACACTTCATGCTTCCCTTAGATCCCTTCTCCCTCTTCACTTCATCAAGGTGAAATATATCATATCTATTTTAATCATTAAAGTTTTCTAGGTTATCATCTGTTATAACTTTAGGGTTATTTATTGTTTCTCTAACAGGGAAAACGTTCTTTATCTGATCAAATGAATGAGGCAGTGAATTACATAAATCACTTGAAGAAGAATATGGAAGAACTAAGTTACAAAAGGGATGAACTGAAAAAACTCTCTAATCCTAGCCTTAAAAATAAAAGCCATGTTTCTTGTAGTTTCACTATCCACAAGAATAATAGAACTGTGGGAATTGAAATCAGTACTAGTACTTGCTTTATAGAGGAAGGTGCCCCACTTTCTAAATTTCTGGAACAACTGATGAAATATGGACTTGATGTTGTTAGCTGCTTTTCTATTCAAGTCAATGGAAGATTACTCCATAGTGTGCAGTGTGAGGTATATATTGTTTACATATTTTTTTTAATTGTCAAAACATGTACATACTCTAATTAATATTTTGAGTTTTTTCTTTAATTATTGAGCTTGATGATTCACAGGTTATCGATTCCGACAGTGTAGATCTAACTGAGCTAAGAAGGAATTTTTCCAATATGAATCCATCATTTAGTTGTTCAGATTAA

Protein sequence

>MS.gene34422.t1

MFPLQRCNELAKPLSNSLNHHPQHHKISEDLILDDCDSLVIDFSLKKMDTNRPPNKLFYIDRTNHGSNRNSIEDKKKMVHREIEKQRRQEMATLHASLRSLLPLHFIKGKRSLSDQMNEAVNYINHLKKNMEELSYKRDELKKLSNPSLKNKSHVSCSFTIHKNNRTVGIEISTSTCFIEEGAPLSKFLEQLMKYGLDVVSCFSIQVNGRLLHSVQCEVIDSDSVDLTELRRNFSNMNPSFSCSD