Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34754.t1 | PNX58374.1 | 100 | 39 | 0 | 0 | 1 | 39 | 14 | 52 | 3.90E-11 | 77.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34754.t1 | A0A2K3JWH9 | 100.0 | 39 | 0 | 0 | 1 | 39 | 14 | 52 | 2.8e-11 | 77.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057622 | MS.gene34754 | 0.817664 | 2.93E-52 | -1.69E-46 |
| MS.gene058592 | MS.gene34754 | 0.806303 | 8.78E-50 | -1.69E-46 |
| MS.gene05952 | MS.gene34754 | 0.803852 | 2.86E-49 | -1.69E-46 |
| MS.gene06023 | MS.gene34754 | 0.804854 | 1.77E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34754.t1 | MTR_8g469140 | 98.374 | 123 | 2 | 0 | 1 | 123 | 175 | 297 | 2.63e-84 | 248 |
| MS.gene34754.t1 | MTR_8g469090 | 78.102 | 137 | 15 | 1 | 1 | 122 | 175 | 311 | 1.75e-65 | 201 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAACTTGCTGAACTAAAAT+TGG | 0.202271 | 8.3:+41259262 | MS.gene34754:CDS |
| ACAAGATTTGGAGGAATTAA+AGG | 0.239206 | 8.3:+41259391 | MS.gene34754:CDS |
| TACGTTTAATGCACAAGATT+TGG | 0.258101 | 8.3:+41259379 | MS.gene34754:CDS |
| TTCTAATCCTCCACTTGTTA+TGG | 0.270977 | 8.3:-41259138 | None:intergenic |
| TGGAGGAATTAAAGGCTGTT+AGG | 0.349015 | 8.3:+41259399 | MS.gene34754:CDS |
| ATCAAATGACTCAAGTGATT+GGG | 0.368083 | 8.3:+41259298 | MS.gene34754:CDS |
| AATCAAATGACTCAAGTGAT+TGG | 0.374132 | 8.3:+41259297 | MS.gene34754:CDS |
| GAGCAATTCAATAGTAAGAT+GGG | 0.477868 | 8.3:+41259446 | MS.gene34754:CDS |
| CCCTCTGAATATGCAATACA+TGG | 0.482852 | 8.3:-41259424 | None:intergenic |
| GGAGCAATTCAATAGTAAGA+TGG | 0.495019 | 8.3:+41259445 | MS.gene34754:CDS |
| CAGCAAGATTACCTGTCCTA+AGG | 0.509159 | 8.3:-41259211 | None:intergenic |
| GTTTAATGCACAAGATTTGG+AGG | 0.529768 | 8.3:+41259382 | MS.gene34754:CDS |
| CTAGAGAAATCCCTTAGGAC+AGG | 0.550277 | 8.3:+41259200 | MS.gene34754:CDS |
| AGCAAGATTACCTGTCCTAA+GGG | 0.568028 | 8.3:-41259210 | None:intergenic |
| TGCAACTTAAACAAGACAAG+TGG | 0.568118 | 8.3:+41259351 | MS.gene34754:CDS |
| GACAGGTAATCTTGCTGACA+TGG | 0.576504 | 8.3:+41259217 | MS.gene34754:CDS |
| TCAAATGACTCAAGTGATTG+GGG | 0.579891 | 8.3:+41259299 | MS.gene34754:CDS |
| AAGCTAGACTCCATAACAAG+TGG | 0.591181 | 8.3:+41259128 | MS.gene34754:CDS |
| ACCATGTATTGCATATTCAG+AGG | 0.614938 | 8.3:+41259423 | MS.gene34754:CDS |
| CTAGACTCCATAACAAGTGG+AGG | 0.615613 | 8.3:+41259131 | MS.gene34754:CDS |
| CCATGTATTGCATATTCAGA+GGG | 0.617852 | 8.3:+41259424 | MS.gene34754:CDS |
| AATAGTAAGATGGGGTTCCG+TGG | 0.631426 | 8.3:+41259455 | MS.gene34754:CDS |
| AGCAATTCAATAGTAAGATG+GGG | 0.673647 | 8.3:+41259447 | MS.gene34754:CDS |
| CTGTATCTATCTATATACCA+CGG | 0.700593 | 8.3:-41259472 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAACTAGAGAAATCCCTT+AGG | + | chr8.3:41259195-41259214 | MS.gene34754:CDS | 30.0% | |
| AATCAAATGACTCAAGTGAT+TGG | + | chr8.3:41259297-41259316 | MS.gene34754:CDS | 30.0% | |
| ACAAGATTTGGAGGAATTAA+AGG | + | chr8.3:41259391-41259410 | MS.gene34754:CDS | 30.0% | |
| AGCAATTCAATAGTAAGATG+GGG | + | chr8.3:41259447-41259466 | MS.gene34754:CDS | 30.0% | |
| ATCAAATGACTCAAGTGATT+GGG | + | chr8.3:41259298-41259317 | MS.gene34754:CDS | 30.0% | |
| GAGCAATTCAATAGTAAGAT+GGG | + | chr8.3:41259446-41259465 | MS.gene34754:CDS | 30.0% | |
| TACGTTTAATGCACAAGATT+TGG | + | chr8.3:41259379-41259398 | MS.gene34754:CDS | 30.0% | |
| !! | ACTTGAGTCATTTGATTTTG+TGG | - | chr8.3:41259294-41259313 | None:intergenic | 30.0% |
| ACCATGTATTGCATATTCAG+AGG | + | chr8.3:41259423-41259442 | MS.gene34754:CDS | 35.0% | |
| CCATGTATTGCATATTCAGA+GGG | + | chr8.3:41259424-41259443 | MS.gene34754:CDS | 35.0% | |
| GGAGCAATTCAATAGTAAGA+TGG | + | chr8.3:41259445-41259464 | MS.gene34754:CDS | 35.0% | |
| GTTTAATGCACAAGATTTGG+AGG | + | chr8.3:41259382-41259401 | MS.gene34754:CDS | 35.0% | |
| TCAAATGACTCAAGTGATTG+GGG | + | chr8.3:41259299-41259318 | MS.gene34754:CDS | 35.0% | |
| TGCAACTTAAACAAGACAAG+TGG | + | chr8.3:41259351-41259370 | MS.gene34754:CDS | 35.0% | |
| ! | TTCTAATCCTCCACTTGTTA+TGG | - | chr8.3:41259141-41259160 | None:intergenic | 35.0% |
| !! | CCAACTTGCTGAACTAAAAT+TGG | + | chr8.3:41259262-41259281 | MS.gene34754:CDS | 35.0% |
| !!! | CCAATTTTAGTTCAGCAAGT+TGG | - | chr8.3:41259265-41259284 | None:intergenic | 35.0% |
| AAGCTAGACTCCATAACAAG+TGG | + | chr8.3:41259128-41259147 | MS.gene34754:CDS | 40.0% | |
| AGCAAGATTACCTGTCCTAA+GGG | - | chr8.3:41259213-41259232 | None:intergenic | 40.0% | |
| CCCTCTGAATATGCAATACA+TGG | - | chr8.3:41259427-41259446 | None:intergenic | 40.0% | |
| TGGAGGAATTAAAGGCTGTT+AGG | + | chr8.3:41259399-41259418 | MS.gene34754:CDS | 40.0% | |
| AATAGTAAGATGGGGTTCCG+TGG | + | chr8.3:41259455-41259474 | MS.gene34754:CDS | 45.0% | |
| CAGCAAGATTACCTGTCCTA+AGG | - | chr8.3:41259214-41259233 | None:intergenic | 45.0% | |
| CTAGACTCCATAACAAGTGG+AGG | + | chr8.3:41259131-41259150 | MS.gene34754:CDS | 45.0% | |
| CTAGAGAAATCCCTTAGGAC+AGG | + | chr8.3:41259200-41259219 | MS.gene34754:CDS | 45.0% | |
| GACAGGTAATCTTGCTGACA+TGG | + | chr8.3:41259217-41259236 | MS.gene34754:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 41259113 | 41259484 | 41259113 | ID=MS.gene34754 |
| chr8.3 | mRNA | 41259113 | 41259484 | 41259113 | ID=MS.gene34754.t1;Parent=MS.gene34754 |
| chr8.3 | exon | 41259113 | 41259484 | 41259113 | ID=MS.gene34754.t1.exon1;Parent=MS.gene34754.t1 |
| chr8.3 | CDS | 41259113 | 41259484 | 41259113 | ID=cds.MS.gene34754.t1;Parent=MS.gene34754.t1 |
| Gene Sequence |
| Protein sequence |
