Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35035.t1 | XP_013457209.1 | 92.3 | 183 | 8 | 2 | 1 | 181 | 1 | 179 | 4.60E-85 | 323.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35035.t1 | Q9SDN0 | 37.6 | 133 | 71 | 4 | 55 | 178 | 66 | 195 | 2.8e-14 | 80.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35035.t1 | A0A072UZE7 | 92.3 | 183 | 8 | 2 | 1 | 181 | 1 | 179 | 3.3e-85 | 323.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051341 | MS.gene35035 | 0.969516 | 3.08E-130 | -1.69E-46 |
| MS.gene052053 | MS.gene35035 | 0.829719 | 4.40E-55 | -1.69E-46 |
| MS.gene052560 | MS.gene35035 | 0.808 | 3.84E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35035.t1 | MTR_4g094275 | 92.350 | 183 | 8 | 2 | 1 | 181 | 1 | 179 | 3.12e-110 | 312 |
| MS.gene35035.t1 | MTR_2g008520 | 56.522 | 161 | 48 | 6 | 5 | 161 | 6 | 148 | 1.85e-46 | 150 |
| MS.gene35035.t1 | MTR_2g008540 | 56.522 | 161 | 48 | 6 | 5 | 161 | 6 | 148 | 1.85e-46 | 150 |
| MS.gene35035.t1 | MTR_8g081620 | 34.211 | 190 | 97 | 9 | 9 | 175 | 5 | 189 | 1.00e-21 | 87.8 |
| MS.gene35035.t1 | MTR_3g061170 | 42.553 | 94 | 48 | 4 | 39 | 128 | 42 | 133 | 1.80e-12 | 62.4 |
| MS.gene35035.t1 | MTR_2g097690 | 41.026 | 78 | 41 | 2 | 45 | 122 | 65 | 137 | 5.27e-12 | 62.0 |
| MS.gene35035.t1 | MTR_2g014470 | 41.250 | 80 | 42 | 3 | 53 | 130 | 2 | 78 | 7.95e-12 | 62.8 |
| MS.gene35035.t1 | MTR_7g085600 | 33.333 | 150 | 88 | 4 | 1 | 146 | 3 | 144 | 1.55e-11 | 62.0 |
| MS.gene35035.t1 | MTR_2g097690 | 41.026 | 78 | 41 | 2 | 45 | 122 | 65 | 137 | 1.78e-11 | 61.6 |
| MS.gene35035.t1 | MTR_5g012530 | 45.455 | 66 | 35 | 1 | 56 | 121 | 13 | 77 | 1.84e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35035.t1 | AT4G13830 | 36.709 | 158 | 83 | 7 | 31 | 175 | 39 | 192 | 1.73e-21 | 87.0 |
| MS.gene35035.t1 | AT4G13830 | 47.222 | 72 | 36 | 2 | 55 | 126 | 66 | 135 | 5.30e-14 | 66.6 |
| MS.gene35035.t1 | AT4G13830 | 47.222 | 72 | 36 | 2 | 55 | 126 | 66 | 135 | 7.84e-14 | 66.6 |
| MS.gene35035.t1 | AT3G62600 | 46.753 | 77 | 35 | 3 | 53 | 127 | 24 | 96 | 1.05e-11 | 62.8 |
| MS.gene35035.t1 | AT2G20560 | 37.500 | 80 | 45 | 3 | 53 | 130 | 2 | 78 | 1.95e-11 | 61.6 |
| MS.gene35035.t1 | AT1G28210 | 40.426 | 94 | 49 | 4 | 55 | 147 | 48 | 135 | 3.02e-11 | 61.6 |
| MS.gene35035.t1 | AT1G28210 | 40.426 | 94 | 49 | 4 | 55 | 147 | 48 | 135 | 3.39e-11 | 61.2 |
| MS.gene35035.t1 | AT1G28210 | 40.426 | 94 | 49 | 4 | 55 | 147 | 48 | 135 | 4.52e-11 | 60.8 |
| MS.gene35035.t1 | AT2G22360 | 33.803 | 142 | 71 | 7 | 2 | 135 | 37 | 163 | 4.54e-11 | 60.8 |
| MS.gene35035.t1 | AT4G28480 | 36.905 | 84 | 48 | 3 | 53 | 134 | 2 | 82 | 6.78e-11 | 60.1 |
| MS.gene35035.t1 | AT4G28480 | 36.905 | 84 | 48 | 3 | 53 | 134 | 2 | 82 | 7.99e-11 | 60.1 |
Find 28 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAACAATATGATAGTGAATT+AGG | 0.232905 | 4.4:-20683292 | MS.gene35035:CDS |
| GTAGAAATTTGATCCATTCT+TGG | 0.275553 | 4.4:+20683495 | None:intergenic |
| TTTAGTTGATTCCTCTTTCA+TGG | 0.279301 | 4.4:+20683369 | None:intergenic |
| AATGCTTAGGCTTGAGGTTA+AGG | 0.338721 | 4.4:+20683630 | None:intergenic |
| AGTAGACGTATGGGACAAAA+AGG | 0.403585 | 4.4:-20683172 | MS.gene35035:CDS |
| ATATTGTAAATGATGAGATA+TGG | 0.436536 | 4.4:-20683240 | MS.gene35035:CDS |
| AAGTGGTTGAGTTGAAGAAA+AGG | 0.458554 | 4.4:-20683198 | MS.gene35035:CDS |
| ATGATGAGATATGGAGAAGT+AGG | 0.468838 | 4.4:-20683231 | MS.gene35035:CDS |
| AAATTTCTACAAAATGTTGT+CGG | 0.490774 | 4.4:-20683482 | MS.gene35035:CDS |
| AAAGAGAGCTTATAGATCAA+TGG | 0.492226 | 4.4:-20683428 | MS.gene35035:CDS |
| GAAAAGGTCTAGTAGACGTA+TGG | 0.496414 | 4.4:-20683182 | MS.gene35035:CDS |
| GCTTTGAATTTGAAATGCTT+AGG | 0.497473 | 4.4:+20683617 | None:intergenic |
| TCATGATCCTTCCATGAAAG+AGG | 0.504521 | 4.4:-20683380 | MS.gene35035:CDS |
| ATTTGAAATGCTTAGGCTTG+AGG | 0.508030 | 4.4:+20683624 | None:intergenic |
| GTTGATTCCTCTTTCATGGA+AGG | 0.513452 | 4.4:+20683373 | None:intergenic |
| GAATCCGAAAAGCGCAACAA+TGG | 0.519653 | 4.4:-20683458 | MS.gene35035:CDS |
| GAAGGATCATGACACACATC+AGG | 0.542719 | 4.4:+20683391 | None:intergenic |
| CTAGTGGAAGAAACCAAGAA+TGG | 0.557604 | 4.4:-20683508 | MS.gene35035:CDS |
| TCATATTGTTCACGAAGCAT+AGG | 0.565520 | 4.4:+20683304 | None:intergenic |
| ATGTAGAGCAACAAAGCTAG+TGG | 0.571091 | 4.4:-20683524 | MS.gene35035:CDS |
| GGACACAAAGCATGAAAGAT+CGG | 0.571745 | 4.4:-20683126 | MS.gene35035:CDS |
| GGTCTTGGGGTAGCAGAATG+AGG | 0.576046 | 4.4:-20683147 | MS.gene35035:CDS |
| AAAAGGTCTAGTAGACGTAT+GGG | 0.582302 | 4.4:-20683181 | MS.gene35035:CDS |
| AGCTGTTTACTAGAGAGTTG+TGG | 0.586525 | 4.4:+20683580 | None:intergenic |
| AAGTAGGTGGCAAGAACAAG+TGG | 0.619538 | 4.4:-20683215 | MS.gene35035:CDS |
| GTTGCAAAGTACACGTGAGT+AGG | 0.624184 | 4.4:+20683553 | None:intergenic |
| ATGAGATATGGAGAAGTAGG+TGG | 0.638622 | 4.4:-20683228 | MS.gene35035:CDS |
| GTTTACTAGAGAGTTGTGGA+AGG | 0.676026 | 4.4:+20683584 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATCAACTAAAATTTTTGTT+CGG | - | chr4.4:20683406-20683425 | MS.gene35035:CDS | 15.0% |
| !! | AAATTTCTACAAAATGTTGT+CGG | - | chr4.4:20683281-20683300 | MS.gene35035:CDS | 20.0% |
| !! | ATATTGTAAATGATGAGATA+TGG | - | chr4.4:20683523-20683542 | MS.gene35035:CDS | 20.0% |
| !! | GAACAATATGATAGTGAATT+AGG | - | chr4.4:20683471-20683490 | MS.gene35035:CDS | 25.0% |
| AAAGAGAGCTTATAGATCAA+TGG | - | chr4.4:20683335-20683354 | MS.gene35035:CDS | 30.0% | |
| GTAGAAATTTGATCCATTCT+TGG | + | chr4.4:20683271-20683290 | None:intergenic | 30.0% | |
| ! | GCTTTGAATTTGAAATGCTT+AGG | + | chr4.4:20683149-20683168 | None:intergenic | 30.0% |
| ! | TTTAGTTGATTCCTCTTTCA+TGG | + | chr4.4:20683397-20683416 | None:intergenic | 30.0% |
| AAAAGGTCTAGTAGACGTAT+GGG | - | chr4.4:20683582-20683601 | MS.gene35035:CDS | 35.0% | |
| ATGATGAGATATGGAGAAGT+AGG | - | chr4.4:20683532-20683551 | MS.gene35035:CDS | 35.0% | |
| TCATATTGTTCACGAAGCAT+AGG | + | chr4.4:20683462-20683481 | None:intergenic | 35.0% | |
| ! | ATTTGAAATGCTTAGGCTTG+AGG | + | chr4.4:20683142-20683161 | None:intergenic | 35.0% |
| !! | AAGTGGTTGAGTTGAAGAAA+AGG | - | chr4.4:20683565-20683584 | MS.gene35035:CDS | 35.0% |
| AATGCTTAGGCTTGAGGTTA+AGG | + | chr4.4:20683136-20683155 | None:intergenic | 40.0% | |
| AGCTGTTTACTAGAGAGTTG+TGG | + | chr4.4:20683186-20683205 | None:intergenic | 40.0% | |
| AGTAGACGTATGGGACAAAA+AGG | - | chr4.4:20683591-20683610 | MS.gene35035:CDS | 40.0% | |
| ATGAGATATGGAGAAGTAGG+TGG | - | chr4.4:20683535-20683554 | MS.gene35035:CDS | 40.0% | |
| ATGTAGAGCAACAAAGCTAG+TGG | - | chr4.4:20683239-20683258 | MS.gene35035:CDS | 40.0% | |
| CTAGTGGAAGAAACCAAGAA+TGG | - | chr4.4:20683255-20683274 | MS.gene35035:CDS | 40.0% | |
| GAAAAGGTCTAGTAGACGTA+TGG | - | chr4.4:20683581-20683600 | MS.gene35035:CDS | 40.0% | |
| GGACACAAAGCATGAAAGAT+CGG | - | chr4.4:20683637-20683656 | MS.gene35035:CDS | 40.0% | |
| GTTTACTAGAGAGTTGTGGA+AGG | + | chr4.4:20683182-20683201 | None:intergenic | 40.0% | |
| TCATGATCCTTCCATGAAAG+AGG | - | chr4.4:20683383-20683402 | MS.gene35035:CDS | 40.0% | |
| ! | GTTGATTCCTCTTTCATGGA+AGG | + | chr4.4:20683393-20683412 | None:intergenic | 40.0% |
| ! | TCTTCCATTGTTGCGCTTTT+CGG | + | chr4.4:20683312-20683331 | None:intergenic | 40.0% |
| AAGTAGGTGGCAAGAACAAG+TGG | - | chr4.4:20683548-20683567 | MS.gene35035:CDS | 45.0% | |
| GAAGGATCATGACACACATC+AGG | + | chr4.4:20683375-20683394 | None:intergenic | 45.0% | |
| GACGTATGGGACAAAAAGGA+AGG | - | chr4.4:20683595-20683614 | MS.gene35035:CDS | 45.0% | |
| GGACAAAAAGGAAGGTCTTG+GGG | - | chr4.4:20683603-20683622 | MS.gene35035:CDS | 45.0% | |
| GGGACAAAAAGGAAGGTCTT+GGG | - | chr4.4:20683602-20683621 | MS.gene35035:CDS | 45.0% | |
| GTTGCAAAGTACACGTGAGT+AGG | + | chr4.4:20683213-20683232 | None:intergenic | 45.0% | |
| TGGGACAAAAAGGAAGGTCT+TGG | - | chr4.4:20683601-20683620 | MS.gene35035:CDS | 45.0% | |
| ! | GAATCCGAAAAGCGCAACAA+TGG | - | chr4.4:20683305-20683324 | MS.gene35035:CDS | 45.0% |
| GGTCTTGGGGTAGCAGAATG+AGG | - | chr4.4:20683616-20683635 | MS.gene35035:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 20683120 | 20683665 | 20683120 | ID=MS.gene35035 |
| chr4.4 | mRNA | 20683120 | 20683665 | 20683120 | ID=MS.gene35035.t1;Parent=MS.gene35035 |
| chr4.4 | exon | 20683120 | 20683665 | 20683120 | ID=MS.gene35035.t1.exon1;Parent=MS.gene35035.t1 |
| chr4.4 | CDS | 20683120 | 20683665 | 20683120 | ID=cds.MS.gene35035.t1;Parent=MS.gene35035.t1 |
| Gene Sequence |
| Protein sequence |
