Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052560.t1 | XP_003608243.1 | 91.5 | 470 | 40 | 0 | 1 | 470 | 59 | 528 | 1.70E-259 | 904.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052560.t1 | Q9FZC5 | 55.7 | 465 | 200 | 4 | 4 | 466 | 65 | 525 | 1.2e-152 | 541.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052560.t1 | Q2HTY4 | 91.5 | 470 | 40 | 0 | 1 | 470 | 59 | 528 | 1.2e-259 | 904.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051341 | MS.gene052560 | 0.809313 | 2.01E-50 | -1.69E-46 |
| MS.gene05156 | MS.gene052560 | 0.816584 | 5.13E-52 | -1.69E-46 |
| MS.gene052053 | MS.gene052560 | 0.812942 | 3.29E-51 | -1.69E-46 |
| MS.gene052559 | MS.gene052560 | 0.912229 | 2.47E-83 | -1.69E-46 |
| MS.gene052560 | MS.gene056711 | 0.807331 | 5.32E-50 | -1.69E-46 |
| MS.gene052560 | MS.gene065380 | 0.806361 | 8.54E-50 | -1.69E-46 |
| MS.gene052560 | MS.gene066723 | 0.924631 | 5.44E-90 | -1.69E-46 |
| MS.gene052560 | MS.gene066724 | 0.893157 | 8.16E-75 | -1.69E-46 |
| MS.gene052560 | MS.gene072227 | 0.841261 | 5.29E-58 | -1.69E-46 |
| MS.gene052560 | MS.gene074124 | 0.811865 | 5.65E-51 | -1.69E-46 |
| MS.gene052560 | MS.gene20344 | 0.834988 | 2.18E-56 | -1.69E-46 |
| MS.gene052560 | MS.gene20459 | 0.808345 | 3.24E-50 | -1.69E-46 |
| MS.gene052560 | MS.gene24408 | 0.809788 | 1.59E-50 | -1.69E-46 |
| MS.gene052560 | MS.gene25379 | 0.800239 | 1.58E-48 | -1.69E-46 |
| MS.gene052560 | MS.gene25530 | 0.813751 | 2.18E-51 | -1.69E-46 |
| MS.gene052560 | MS.gene32207 | 0.909704 | 4.24E-82 | -1.69E-46 |
| MS.gene052560 | MS.gene32208 | 0.981742 | 2.46E-153 | -1.69E-46 |
| MS.gene052560 | MS.gene35035 | 0.808 | 3.84E-50 | -1.69E-46 |
| MS.gene052560 | MS.gene36749 | 0.81136 | 7.28E-51 | -1.69E-46 |
| MS.gene052560 | MS.gene39054 | 0.842514 | 2.47E-58 | -1.69E-46 |
| MS.gene052560 | MS.gene42537 | 0.839651 | 1.39E-57 | -1.69E-46 |
| MS.gene052560 | MS.gene43751 | 0.821704 | 3.50E-53 | -1.69E-46 |
| MS.gene052560 | MS.gene44854 | 0.811681 | 6.20E-51 | -1.69E-46 |
| MS.gene052560 | MS.gene45552 | 0.811654 | 6.28E-51 | -1.69E-46 |
| MS.gene052560 | MS.gene47049 | 0.844177 | 8.89E-59 | -1.69E-46 |
| MS.gene052560 | MS.gene47421 | 0.811907 | 5.53E-51 | -1.69E-46 |
| MS.gene052560 | MS.gene47423 | 0.828955 | 6.74E-55 | -1.69E-46 |
| MS.gene052560 | MS.gene49911 | 0.822335 | 2.50E-53 | -1.69E-46 |
| MS.gene052560 | MS.gene51528 | 0.801733 | 7.83E-49 | -1.69E-46 |
| MS.gene052560 | MS.gene54952 | 0.80686 | 6.70E-50 | -1.69E-46 |
| MS.gene052560 | MS.gene56769 | 0.83155 | 1.57E-55 | -1.69E-46 |
| MS.gene052560 | MS.gene63175 | 0.82781 | 1.27E-54 | -1.69E-46 |
| MS.gene052560 | MS.gene63511 | 0.805004 | 1.65E-49 | -1.69E-46 |
| MS.gene052560 | MS.gene65297 | 0.821433 | 4.05E-53 | -1.69E-46 |
| MS.gene052560 | MS.gene65364 | 0.808118 | 3.62E-50 | -1.69E-46 |
| MS.gene052560 | MS.gene65365 | 0.812534 | 4.04E-51 | -1.69E-46 |
| MS.gene052560 | MS.gene66360 | 0.810567 | 1.08E-50 | -1.69E-46 |
| MS.gene052560 | MS.gene67131 | 0.81558 | 8.59E-52 | -1.69E-46 |
| MS.gene052560 | MS.gene67744 | 0.803637 | 3.17E-49 | -1.69E-46 |
| MS.gene052560 | MS.gene73236 | 0.829935 | 3.90E-55 | -1.69E-46 |
| MS.gene052560 | MS.gene73644 | 0.831068 | 2.06E-55 | -1.69E-46 |
| MS.gene052560 | MS.gene73753 | 0.818638 | 1.77E-52 | -1.69E-46 |
| MS.gene052560 | MS.gene76262 | 0.809799 | 1.58E-50 | -1.69E-46 |
| MS.gene052560 | MS.gene78327 | 0.828799 | 7.35E-55 | -1.69E-46 |
| MS.gene052560 | MS.gene78885 | 0.81931 | 1.24E-52 | -1.69E-46 |
| MS.gene052560 | MS.gene79433 | 0.836166 | 1.10E-56 | -1.69E-46 |
| MS.gene052560 | MS.gene80055 | 0.84136 | 4.98E-58 | -1.69E-46 |
| MS.gene052560 | MS.gene80056 | 0.809415 | 1.91E-50 | -1.69E-46 |
| MS.gene052560 | MS.gene81359 | 0.811558 | 6.59E-51 | -1.69E-46 |
| MS.gene052560 | MS.gene83191 | 0.812406 | 4.31E-51 | -1.69E-46 |
| MS.gene052560 | MS.gene83576 | -0.808306 | 3.30E-50 | -1.69E-46 |
| MS.gene052560 | MS.gene89702 | 0.806517 | 7.92E-50 | -1.69E-46 |
| MS.gene052560 | MS.gene90822 | 0.827899 | 1.21E-54 | -1.69E-46 |
| MS.gene052560 | MS.gene92589 | 0.821015 | 5.06E-53 | -1.69E-46 |
| MS.gene052560 | MS.gene94506 | 0.807409 | 5.13E-50 | -1.69E-46 |
| MS.gene052560 | MS.gene97813 | 0.842686 | 2.22E-58 | -1.69E-46 |
| MS.gene052560 | MS.gene97814 | 0.817985 | 2.48E-52 | -1.69E-46 |
| MS.gene052560 | MS.gene98636 | 0.813119 | 3.01E-51 | -1.69E-46 |
| MS.gene052560 | MS.gene99191 | 0.821109 | 4.81E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052560.t1 | MTR_4g091200 | 91.489 | 470 | 40 | 0 | 1 | 470 | 59 | 528 | 0.0 | 883 |
| MS.gene052560.t1 | MTR_6g017205 | 90.254 | 472 | 46 | 0 | 1 | 472 | 59 | 530 | 0.0 | 865 |
| MS.gene052560.t1 | MTR_4g057990 | 82.303 | 469 | 79 | 2 | 1 | 469 | 59 | 523 | 0.0 | 780 |
| MS.gene052560.t1 | MTR_4g091190 | 80.128 | 468 | 91 | 1 | 1 | 468 | 63 | 528 | 0.0 | 763 |
| MS.gene052560.t1 | MTR_4g088355 | 78.678 | 469 | 100 | 0 | 1 | 469 | 66 | 534 | 0.0 | 759 |
| MS.gene052560.t1 | MTR_2g023590 | 79.281 | 473 | 96 | 1 | 1 | 473 | 1 | 471 | 0.0 | 756 |
| MS.gene052560.t1 | MTR_4g091170 | 73.203 | 459 | 105 | 1 | 10 | 468 | 102 | 542 | 0.0 | 709 |
| MS.gene052560.t1 | MTR_4g091150 | 63.248 | 468 | 170 | 2 | 1 | 466 | 71 | 538 | 0.0 | 597 |
| MS.gene052560.t1 | MTR_2g031560 | 54.077 | 466 | 211 | 2 | 3 | 467 | 59 | 522 | 0.0 | 541 |
| MS.gene052560.t1 | MTR_2g031590 | 53.291 | 471 | 211 | 4 | 3 | 467 | 64 | 531 | 4.08e-179 | 513 |
| MS.gene052560.t1 | MTR_4g094498 | 53.333 | 465 | 213 | 3 | 3 | 467 | 73 | 533 | 6.95e-173 | 497 |
| MS.gene052560.t1 | MTR_4g094488 | 53.700 | 473 | 210 | 4 | 3 | 468 | 67 | 537 | 1.79e-165 | 478 |
| MS.gene052560.t1 | MTR_4g094495 | 52.754 | 472 | 214 | 4 | 4 | 468 | 71 | 540 | 4.87e-165 | 477 |
| MS.gene052560.t1 | MTR_4g094492 | 51.386 | 469 | 222 | 3 | 4 | 467 | 68 | 535 | 1.60e-163 | 473 |
| MS.gene052560.t1 | MTR_2g031390 | 49.465 | 467 | 228 | 6 | 4 | 466 | 65 | 527 | 1.99e-163 | 473 |
| MS.gene052560.t1 | MTR_4g094480 | 52.239 | 469 | 219 | 3 | 4 | 468 | 68 | 535 | 6.43e-163 | 471 |
| MS.gene052560.t1 | MTR_4g094478 | 52.564 | 468 | 217 | 3 | 4 | 467 | 68 | 534 | 3.87e-162 | 469 |
| MS.gene052560.t1 | MTR_4g094520 | 50.959 | 469 | 219 | 6 | 4 | 467 | 69 | 531 | 1.01e-159 | 463 |
| MS.gene052560.t1 | MTR_3g009820 | 48.491 | 464 | 232 | 5 | 4 | 466 | 63 | 520 | 2.17e-159 | 462 |
| MS.gene052560.t1 | MTR_2g031490 | 51.064 | 470 | 220 | 6 | 3 | 466 | 71 | 536 | 1.48e-157 | 458 |
| MS.gene052560.t1 | MTR_4g094518 | 49.680 | 469 | 222 | 7 | 5 | 466 | 67 | 528 | 6.91e-157 | 456 |
| MS.gene052560.t1 | MTR_2g031510 | 50.105 | 475 | 217 | 6 | 3 | 466 | 73 | 538 | 3.94e-156 | 454 |
| MS.gene052560.t1 | MTR_3g009760 | 47.414 | 464 | 237 | 5 | 4 | 466 | 63 | 520 | 3.05e-153 | 446 |
| MS.gene052560.t1 | MTR_2g031420 | 50.641 | 468 | 224 | 4 | 3 | 469 | 67 | 528 | 8.12e-153 | 445 |
| MS.gene052560.t1 | MTR_3g009880 | 48.491 | 464 | 232 | 5 | 4 | 466 | 61 | 518 | 3.26e-152 | 444 |
| MS.gene052560.t1 | MTR_4g094418 | 50.342 | 439 | 210 | 4 | 40 | 471 | 1 | 438 | 5.76e-152 | 446 |
| MS.gene052560.t1 | MTR_2g031460 | 49.353 | 464 | 228 | 4 | 3 | 465 | 67 | 524 | 3.19e-151 | 441 |
| MS.gene052560.t1 | MTR_4g094515 | 51.592 | 471 | 219 | 6 | 3 | 468 | 74 | 540 | 7.40e-150 | 456 |
| MS.gene052560.t1 | MTR_4g094515 | 51.496 | 468 | 220 | 5 | 3 | 467 | 686 | 1149 | 6.43e-148 | 451 |
| MS.gene052560.t1 | MTR_1g076830 | 37.917 | 480 | 282 | 8 | 3 | 473 | 68 | 540 | 3.62e-101 | 313 |
| MS.gene052560.t1 | MTR_2g031600 | 64.444 | 180 | 64 | 0 | 3 | 182 | 65 | 244 | 3.21e-71 | 231 |
| MS.gene052560.t1 | MTR_2g031600 | 51.875 | 160 | 76 | 1 | 308 | 467 | 241 | 399 | 3.30e-53 | 184 |
| MS.gene052560.t1 | MTR_3g009850 | 48.661 | 224 | 112 | 3 | 244 | 466 | 5 | 226 | 9.93e-65 | 209 |
| MS.gene052560.t1 | MTR_2g084370 | 46.847 | 111 | 37 | 2 | 64 | 174 | 1 | 89 | 5.71e-29 | 110 |
| MS.gene052560.t1 | MTR_2g084405 | 45.570 | 79 | 43 | 0 | 64 | 142 | 1 | 79 | 4.07e-16 | 74.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052560.t1 | AT4G20800 | 55.365 | 466 | 205 | 2 | 4 | 469 | 64 | 526 | 9.44e-178 | 509 |
| MS.gene052560.t1 | AT1G26420 | 54.957 | 464 | 203 | 4 | 4 | 466 | 65 | 523 | 4.33e-176 | 504 |
| MS.gene052560.t1 | AT1G26380 | 55.172 | 464 | 202 | 4 | 4 | 466 | 65 | 523 | 5.06e-175 | 502 |
| MS.gene052560.t1 | AT1G26390 | 55.699 | 465 | 200 | 4 | 4 | 466 | 65 | 525 | 2.07e-174 | 500 |
| MS.gene052560.t1 | AT1G30720 | 53.632 | 468 | 212 | 3 | 2 | 468 | 63 | 526 | 1.05e-173 | 498 |
| MS.gene052560.t1 | AT1G34575 | 54.390 | 467 | 201 | 4 | 3 | 466 | 65 | 522 | 8.83e-172 | 493 |
| MS.gene052560.t1 | AT1G30730 | 52.350 | 468 | 218 | 3 | 2 | 468 | 62 | 525 | 4.52e-171 | 492 |
| MS.gene052560.t1 | AT1G26400 | 53.205 | 468 | 213 | 6 | 4 | 470 | 65 | 527 | 1.23e-169 | 488 |
| MS.gene052560.t1 | AT1G30710 | 54.025 | 472 | 202 | 7 | 3 | 469 | 68 | 529 | 1.29e-168 | 486 |
| MS.gene052560.t1 | AT1G26410 | 54.741 | 464 | 204 | 5 | 4 | 466 | 89 | 547 | 1.68e-168 | 486 |
| MS.gene052560.t1 | AT1G30700 | 51.923 | 468 | 216 | 5 | 3 | 466 | 62 | 524 | 5.45e-163 | 471 |
| MS.gene052560.t1 | AT5G44400 | 49.465 | 467 | 228 | 6 | 4 | 466 | 72 | 534 | 3.24e-157 | 457 |
| MS.gene052560.t1 | AT5G44390 | 50.107 | 469 | 226 | 5 | 4 | 467 | 74 | 539 | 6.50e-157 | 456 |
| MS.gene052560.t1 | AT2G34810 | 49.362 | 470 | 232 | 3 | 3 | 467 | 70 | 538 | 1.68e-155 | 452 |
| MS.gene052560.t1 | AT2G34790 | 49.359 | 468 | 227 | 5 | 4 | 468 | 70 | 530 | 1.79e-155 | 452 |
| MS.gene052560.t1 | AT4G20840 | 50.638 | 470 | 219 | 7 | 3 | 467 | 73 | 534 | 2.71e-154 | 449 |
| MS.gene052560.t1 | AT1G30760 | 49.359 | 468 | 225 | 6 | 4 | 467 | 72 | 531 | 1.65e-152 | 445 |
| MS.gene052560.t1 | AT1G30760 | 49.359 | 468 | 225 | 6 | 4 | 467 | 90 | 549 | 2.13e-152 | 445 |
| MS.gene052560.t1 | AT1G11770 | 48.827 | 469 | 229 | 6 | 3 | 467 | 67 | 528 | 2.72e-149 | 437 |
| MS.gene052560.t1 | AT5G44380 | 49.142 | 466 | 231 | 4 | 4 | 466 | 75 | 537 | 1.41e-147 | 432 |
| MS.gene052560.t1 | AT5G44380 | 49.142 | 466 | 231 | 4 | 4 | 466 | 115 | 577 | 3.71e-147 | 433 |
| MS.gene052560.t1 | AT1G01980 | 50.737 | 475 | 216 | 9 | 3 | 467 | 67 | 533 | 6.13e-147 | 431 |
| MS.gene052560.t1 | AT4G20820 | 47.639 | 466 | 230 | 5 | 3 | 466 | 71 | 524 | 5.51e-145 | 426 |
| MS.gene052560.t1 | AT4G20860 | 47.807 | 456 | 232 | 4 | 12 | 466 | 77 | 527 | 5.76e-145 | 425 |
| MS.gene052560.t1 | AT5G44360 | 47.966 | 467 | 231 | 7 | 4 | 466 | 70 | 528 | 2.97e-140 | 413 |
| MS.gene052560.t1 | AT5G44360 | 47.966 | 467 | 231 | 7 | 4 | 466 | 86 | 544 | 3.52e-140 | 414 |
| MS.gene052560.t1 | AT4G20830 | 49.149 | 470 | 226 | 7 | 3 | 467 | 74 | 535 | 8.45e-140 | 412 |
| MS.gene052560.t1 | AT4G20830 | 49.149 | 470 | 226 | 7 | 3 | 467 | 74 | 535 | 2.68e-139 | 412 |
| MS.gene052560.t1 | AT5G44440 | 46.170 | 470 | 240 | 5 | 3 | 466 | 65 | 527 | 3.87e-138 | 408 |
| MS.gene052560.t1 | AT5G44440 | 46.170 | 470 | 240 | 5 | 3 | 466 | 135 | 597 | 5.46e-137 | 407 |
| MS.gene052560.t1 | AT5G44410 | 48.408 | 471 | 224 | 9 | 3 | 466 | 69 | 527 | 6.99e-135 | 400 |
| MS.gene052560.t1 | AT1G30740 | 47.234 | 470 | 237 | 7 | 3 | 467 | 64 | 527 | 1.27e-129 | 386 |
| MS.gene052560.t1 | AT1G30740 | 47.234 | 470 | 237 | 7 | 3 | 467 | 67 | 530 | 1.73e-129 | 386 |
Find 86 sgRNAs with CRISPR-Local
Find 107 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACCTAATGTTGTTCAAAT+TGG | 0.228780 | 4.3:-22826095 | MS.gene052560:CDS |
| AAGCTGAATGTTGTTGCTTT+TGG | 0.230709 | 4.3:+22826667 | None:intergenic |
| GGAAATTTGATGAGAAAATT+TGG | 0.258916 | 4.3:-22826395 | MS.gene052560:CDS |
| TTTGACATTGCCAAAACTTA+TGG | 0.263954 | 4.3:-22825489 | MS.gene052560:CDS |
| TGGCACATTTAACTGTTTCT+TGG | 0.267359 | 4.3:+22826687 | None:intergenic |
| AATATATCTTTATGCAGTTT+TGG | 0.269973 | 4.3:+22826128 | None:intergenic |
| AAACTTGCTTCCATAAGTTT+TGG | 0.271849 | 4.3:+22825479 | None:intergenic |
| AGTAAGGAATTCCCCGAATT+AGG | 0.285169 | 4.3:-22825993 | MS.gene052560:CDS |
| TCCTTCATCTTTCCACCATA+TGG | 0.288030 | 4.3:+22825741 | None:intergenic |
| AACAAGCTTGCTTTCCCATC+TGG | 0.299204 | 4.3:-22826467 | MS.gene052560:CDS |
| GGGAGGCATTTCTCAATTAT+AGG | 0.301839 | 4.3:-22825554 | MS.gene052560:CDS |
| ACTTTGGTTCAAACCTAATT+CGG | 0.311377 | 4.3:+22825980 | None:intergenic |
| TGACCAATTTGAACAACATT+AGG | 0.312133 | 4.3:+22826092 | None:intergenic |
| ATAGATTCTAGAGCTTCTTT+AGG | 0.318699 | 4.3:+22825810 | None:intergenic |
| TCTAGAGCTTCTTTAGGAAT+AGG | 0.321024 | 4.3:+22825816 | None:intergenic |
| GTTATAATTGCCAAAGGTTT+CGG | 0.327951 | 4.3:+22826731 | None:intergenic |
| GGTGGTGGTGGTGCTAGTTT+TGG | 0.331271 | 4.3:-22826272 | MS.gene052560:CDS |
| AAGAAGCTCTAGAATCTATA+TGG | 0.334429 | 4.3:-22825806 | MS.gene052560:CDS |
| CTTTGGTTCAAACCTAATTC+GGG | 0.343696 | 4.3:+22825981 | None:intergenic |
| CATTTCTCTGGTGGTGGGTA+TGG | 0.360817 | 4.3:-22826416 | MS.gene052560:CDS |
| AATTATAGGGATGCTGATAT+TGG | 0.376157 | 4.3:-22825540 | MS.gene052560:CDS |
| GTAGTGCAAGTTAGTTTCAT+TGG | 0.379275 | 4.3:-22826056 | MS.gene052560:CDS |
| GTGATTGTCTTTCAATGCCT+TGG | 0.383003 | 4.3:-22825959 | MS.gene052560:CDS |
| GGAGGCATTTCTCAATTATA+GGG | 0.389544 | 4.3:-22825553 | MS.gene052560:CDS |
| TCAAATCTCGTTACATTACT+TGG | 0.393941 | 4.3:+22825507 | None:intergenic |
| ACGATAACCTGTGGAGTTAT+CGG | 0.397449 | 4.3:+22826218 | None:intergenic |
| TAAATTGGTTGAGGATCCTT+TGG | 0.399190 | 4.3:+22825870 | None:intergenic |
| TACTCCTCTTGAAGCATTGT+TGG | 0.402290 | 4.3:-22825898 | MS.gene052560:CDS |
| TTCATATCTAAAGAAATTCT+CGG | 0.418836 | 4.3:+22825407 | None:intergenic |
| GAAATTCTCGGGATCAACTT+TGG | 0.424393 | 4.3:+22825419 | None:intergenic |
| GGTGGTCATTTCTCTGGTGG+TGG | 0.426644 | 4.3:-22826422 | MS.gene052560:CDS |
| TCAACAGCATGGGTTGAATC+AGG | 0.427451 | 4.3:-22826533 | MS.gene052560:CDS |
| ACAAGCTTGCTTTCCCATCT+GGG | 0.436569 | 4.3:-22826466 | MS.gene052560:CDS |
| TCTTTCATGGAAACTTCAAT+TGG | 0.441584 | 4.3:-22826243 | MS.gene052560:CDS |
| CTTATGCAATGGAATCCATA+TGG | 0.450449 | 4.3:-22825756 | MS.gene052560:CDS |
| TATTCTTGATAGAAAATCAA+TGG | 0.458066 | 4.3:-22826321 | MS.gene052560:CDS |
| GGTGCTGGTGGTCATTTCTC+TGG | 0.459504 | 4.3:-22826428 | MS.gene052560:CDS |
| TCTGGGGTCTGCTTTACTCT+AGG | 0.464774 | 4.3:-22826449 | MS.gene052560:CDS |
| AGCAACAACACCGAAACCTT+TGG | 0.466175 | 4.3:-22826741 | MS.gene052560:CDS |
| CTCGGAGAATTCGAAACATA+AGG | 0.468696 | 4.3:+22825576 | None:intergenic |
| GTCTGCTTTACTCTAGGTGC+TGG | 0.472600 | 4.3:-22826443 | MS.gene052560:CDS |
| CATATGGATTCCATTGCATA+AGG | 0.477148 | 4.3:+22825757 | None:intergenic |
| CAACTGATGTTGTTTACAAA+TGG | 0.485694 | 4.3:-22826163 | MS.gene052560:CDS |
| AGTGGCGGTCACGACTTTGA+AGG | 0.494173 | 4.3:-22826635 | MS.gene052560:CDS |
| GTGGTCATTTCTCTGGTGGT+GGG | 0.501321 | 4.3:-22826421 | MS.gene052560:CDS |
| AGAGTAAAGCAGACCCCAGA+TGG | 0.503056 | 4.3:+22826452 | None:intergenic |
| GACTTATACAATGATCTAGT+AGG | 0.504206 | 4.3:+22825375 | None:intergenic |
| AACAAGTCAAGTATAACAAA+AGG | 0.504620 | 4.3:+22826590 | None:intergenic |
| AACAAGTTTCCTACTCTATG+AGG | 0.510558 | 4.3:+22825693 | None:intergenic |
| TGCTTTACTCTAGGTGCTGG+TGG | 0.512366 | 4.3:-22826440 | MS.gene052560:CDS |
| TCATATCTAAAGAAATTCTC+GGG | 0.512401 | 4.3:+22825408 | None:intergenic |
| TGGGCTATAAGAGGTGGTGG+TGG | 0.518497 | 4.3:-22826284 | MS.gene052560:CDS |
| TTTCCTACTCTATGAGGAAA+CGG | 0.519543 | 4.3:+22825699 | None:intergenic |
| GCTTCAAGAGGAGTACCAAT+TGG | 0.520632 | 4.3:+22825906 | None:intergenic |
| ATTCTTGATAGAAAATCAAT+GGG | 0.522100 | 4.3:-22826320 | MS.gene052560:CDS |
| GTGAGAATTTCCTTATGCAA+TGG | 0.526102 | 4.3:-22825767 | MS.gene052560:CDS |
| TCCATATGGTGGAAAGATGA+AGG | 0.526227 | 4.3:-22825742 | MS.gene052560:CDS |
| GTTGTTCAAATTGGTCAAGA+GGG | 0.530168 | 4.3:-22826086 | MS.gene052560:CDS |
| ATTGGTTCCGATAACTCCAC+AGG | 0.531147 | 4.3:-22826225 | MS.gene052560:CDS |
| ATATGGAAATTGATGATTGA+AGG | 0.537491 | 4.3:-22825789 | MS.gene052560:CDS |
| TTTACAAGAATCAACAGCAT+GGG | 0.543499 | 4.3:-22826543 | MS.gene052560:CDS |
| GCATTGAAAGACAATCACTT+TGG | 0.543501 | 4.3:+22825964 | None:intergenic |
| TGATTCAATACTTTAATCCG+TGG | 0.548605 | 4.3:-22825668 | MS.gene052560:CDS |
| TGTTGTTCAAATTGGTCAAG+AGG | 0.558808 | 4.3:-22826087 | MS.gene052560:CDS |
| AGCATTGTTGGAAGAACCAA+AGG | 0.559301 | 4.3:-22825886 | MS.gene052560:CDS |
| GCTGGTGGTCATTTCTCTGG+TGG | 0.564457 | 4.3:-22826425 | MS.gene052560:CDS |
| CAAGCTTGCTTTCCCATCTG+GGG | 0.567506 | 4.3:-22826465 | MS.gene052560:CDS |
| TTTGCAGTTATAATTGCCAA+AGG | 0.570639 | 4.3:+22826725 | None:intergenic |
| TGAGGAAACGGTGTTTCTGA+TGG | 0.577426 | 4.3:+22825711 | None:intergenic |
| GTTGAATCAGGTGCAACACT+TGG | 0.578127 | 4.3:-22826521 | MS.gene052560:CDS |
| TAATTGAGAAATGCCTCCCT+CGG | 0.594640 | 4.3:+22825558 | None:intergenic |
| TCTTCCAACAATGCTTCAAG+AGG | 0.594711 | 4.3:+22825894 | None:intergenic |
| ACACCGTTTCCTCATAGAGT+AGG | 0.600052 | 4.3:-22825702 | MS.gene052560:CDS |
| AAAAGAGTGGAATTAATCCA+AGG | 0.600902 | 4.3:+22825942 | None:intergenic |
| ATTTACAAGAATCAACAGCA+TGG | 0.603350 | 4.3:-22826544 | MS.gene052560:CDS |
| TTATGTTTCGAATTCTCCGA+GGG | 0.609571 | 4.3:-22825574 | MS.gene052560:CDS |
| TGTTTCGAATTCTCCGAGGG+AGG | 0.634404 | 4.3:-22825571 | MS.gene052560:CDS |
| GAGTAAAGCAGACCCCAGAT+GGG | 0.637967 | 4.3:+22826453 | None:intergenic |
| CAGCTTAGAATTCGAAGTGG+CGG | 0.641139 | 4.3:-22826650 | MS.gene052560:CDS |
| TCAGAAGAATCATTTGACCA+CGG | 0.643223 | 4.3:+22825651 | None:intergenic |
| TTTGGTTCAAACCTAATTCG+GGG | 0.645523 | 4.3:+22825982 | None:intergenic |
| ATGCAATGGAATCCATATGG+TGG | 0.646403 | 4.3:-22825753 | MS.gene052560:CDS |
| ACATTGAAAACGATAACCTG+TGG | 0.652393 | 4.3:+22826209 | None:intergenic |
| CTTATGTTTCGAATTCTCCG+AGG | 0.672859 | 4.3:-22825575 | MS.gene052560:CDS |
| GTGAAAAGGAATGTCAGTGA+AGG | 0.694179 | 4.3:-22826188 | MS.gene052560:CDS |
| ATTCAGCTTAGAATTCGAAG+TGG | 0.701549 | 4.3:-22826653 | MS.gene052560:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GATTTATTTTTTTGATAAAT+TGG | + | chr4.3:22826286-22826305 | None:intergenic | 10.0% |
| !! | ATTCTTGATAGAAAATCAAT+GGG | - | chr4.3:22825818-22825837 | MS.gene052560:CDS | 20.0% |
| !! | TATTCTTGATAGAAAATCAA+TGG | - | chr4.3:22825817-22825836 | MS.gene052560:CDS | 20.0% |
| !! | TTCATATCTAAAGAAATTCT+CGG | + | chr4.3:22826734-22826753 | None:intergenic | 20.0% |
| !!! | AATATATCTTTATGCAGTTT+TGG | + | chr4.3:22826013-22826032 | None:intergenic | 20.0% |
| !!! | ACTTTTAATTTTGATGAGTA+AGG | - | chr4.3:22826129-22826148 | MS.gene052560:CDS | 20.0% |
| !!! | TTTTTTTGATAAATTGGTTG+AGG | + | chr4.3:22826280-22826299 | None:intergenic | 20.0% |
| ! | AACAAGTCAAGTATAACAAA+AGG | + | chr4.3:22825551-22825570 | None:intergenic | 25.0% |
| ! | GCAAAAATTGTTGATGTTAA+GGG | - | chr4.3:22825791-22825810 | MS.gene052560:CDS | 25.0% |
| ! | GGAAATTTGATGAGAAAATT+TGG | - | chr4.3:22825743-22825762 | MS.gene052560:CDS | 25.0% |
| ! | TCATATCTAAAGAAATTCTC+GGG | + | chr4.3:22826733-22826752 | None:intergenic | 25.0% |
| ! | TGCAAAAATTGTTGATGTTA+AGG | - | chr4.3:22825790-22825809 | MS.gene052560:CDS | 25.0% |
| !! | AGTTTCATTGGTCAATTTTT+AGG | - | chr4.3:22826094-22826113 | MS.gene052560:CDS | 25.0% |
| !! | ATATGGAAATTGATGATTGA+AGG | - | chr4.3:22826349-22826368 | MS.gene052560:CDS | 25.0% |
| !! | TTATCGTTTTCAATGTGAAA+AGG | - | chr4.3:22825936-22825955 | MS.gene052560:CDS | 25.0% |
| AAAAGAGTGGAATTAATCCA+AGG | + | chr4.3:22826199-22826218 | None:intergenic | 30.0% | |
| AATTATAGGGATGCTGATAT+TGG | - | chr4.3:22826598-22826617 | MS.gene052560:CDS | 30.0% | |
| ATTTACAAGAATCAACAGCA+TGG | - | chr4.3:22825594-22825613 | MS.gene052560:CDS | 30.0% | |
| CAACCTAATGTTGTTCAAAT+TGG | - | chr4.3:22826043-22826062 | MS.gene052560:CDS | 30.0% | |
| GACTTATACAATGATCTAGT+AGG | + | chr4.3:22826766-22826785 | None:intergenic | 30.0% | |
| GTTATAATTGCCAAAGGTTT+CGG | + | chr4.3:22825410-22825429 | None:intergenic | 30.0% | |
| TCAAATCTCGTTACATTACT+TGG | + | chr4.3:22826634-22826653 | None:intergenic | 30.0% | |
| TCTTTCATGGAAACTTCAAT+TGG | - | chr4.3:22825895-22825914 | MS.gene052560:CDS | 30.0% | |
| TGACCAATTTGAACAACATT+AGG | + | chr4.3:22826049-22826068 | None:intergenic | 30.0% | |
| TGATTCAATACTTTAATCCG+TGG | - | chr4.3:22826470-22826489 | MS.gene052560:CDS | 30.0% | |
| TTTACAAGAATCAACAGCAT+GGG | - | chr4.3:22825595-22825614 | MS.gene052560:CDS | 30.0% | |
| TTTGACATTGCCAAAACTTA+TGG | - | chr4.3:22826649-22826668 | MS.gene052560:CDS | 30.0% | |
| TTTGCAGTTATAATTGCCAA+AGG | + | chr4.3:22825416-22825435 | None:intergenic | 30.0% | |
| ! | AACGTCATGTGAATTTTTCA+AGG | - | chr4.3:22826518-22826537 | MS.gene052560:CDS | 30.0% |
| ! | ACTTTGGTTCAAACCTAATT+CGG | + | chr4.3:22826161-22826180 | None:intergenic | 30.0% |
| ! | CAACTGATGTTGTTTACAAA+TGG | - | chr4.3:22825975-22825994 | MS.gene052560:CDS | 30.0% |
| !! | AAACTTGCTTCCATAAGTTT+TGG | + | chr4.3:22826662-22826681 | None:intergenic | 30.0% |
| !! | AAGAAGCTCTAGAATCTATA+TGG | - | chr4.3:22826332-22826351 | MS.gene052560:CDS | 30.0% |
| !! | ATAGATTCTAGAGCTTCTTT+AGG | + | chr4.3:22826331-22826350 | None:intergenic | 30.0% |
| !! | TATGGAAGCAAGTTTTTCAA+AGG | - | chr4.3:22826667-22826686 | MS.gene052560:CDS | 30.0% |
| !!! | AATGGGAGAAGATCTTTTTT+GGG | - | chr4.3:22825835-22825854 | MS.gene052560:CDS | 30.0% |
| !!! | AATTCCACTCTTTTTTGGTA+TGG | - | chr4.3:22826205-22826224 | MS.gene052560:CDS | 30.0% |
| !!! | GGATTAATTCCACTCTTTTT+TGG | - | chr4.3:22826200-22826219 | MS.gene052560:CDS | 30.0% |
| !!! | GTTTTGGTGTTATTCTTTCA+TGG | - | chr4.3:22825882-22825901 | MS.gene052560:CDS | 30.0% |
| AACAAGTTTCCTACTCTATG+AGG | + | chr4.3:22826448-22826467 | None:intergenic | 35.0% | |
| AATTGGTCAAGAGGGAAAAA+AGG | - | chr4.3:22826060-22826079 | MS.gene052560:CDS | 35.0% | |
| ACATTGAAAACGATAACCTG+TGG | + | chr4.3:22825932-22825951 | None:intergenic | 35.0% | |
| ATTCAGCTTAGAATTCGAAG+TGG | - | chr4.3:22825485-22825504 | MS.gene052560:CDS | 35.0% | |
| CATATGGATTCCATTGCATA+AGG | + | chr4.3:22826384-22826403 | None:intergenic | 35.0% | |
| CTTATGCAATGGAATCCATA+TGG | - | chr4.3:22826382-22826401 | MS.gene052560:CDS | 35.0% | |
| GCATTGAAAGACAATCACTT+TGG | + | chr4.3:22826177-22826196 | None:intergenic | 35.0% | |
| GTGAGAATTTCCTTATGCAA+TGG | - | chr4.3:22826371-22826390 | MS.gene052560:CDS | 35.0% | |
| GTTGTTCAAATTGGTCAAGA+GGG | - | chr4.3:22826052-22826071 | MS.gene052560:CDS | 35.0% | |
| TAAATTGGTTGAGGATCCTT+TGG | + | chr4.3:22826271-22826290 | None:intergenic | 35.0% | |
| TCAGAAGAATCATTTGACCA+CGG | + | chr4.3:22826490-22826509 | None:intergenic | 35.0% | |
| TGTTGTTCAAATTGGTCAAG+AGG | - | chr4.3:22826051-22826070 | MS.gene052560:CDS | 35.0% | |
| TTATGTTTCGAATTCTCCGA+GGG | - | chr4.3:22826564-22826583 | MS.gene052560:CDS | 35.0% | |
| TTTCCTACTCTATGAGGAAA+CGG | + | chr4.3:22826442-22826461 | None:intergenic | 35.0% | |
| ! | CTTTGGTTCAAACCTAATTC+GGG | + | chr4.3:22826160-22826179 | None:intergenic | 35.0% |
| ! | GTAGTGCAAGTTAGTTTCAT+TGG | - | chr4.3:22826082-22826101 | MS.gene052560:CDS | 35.0% |
| ! | TCTAGAGCTTCTTTAGGAAT+AGG | + | chr4.3:22826325-22826344 | None:intergenic | 35.0% |
| ! | TGGCACATTTAACTGTTTCT+TGG | + | chr4.3:22825454-22825473 | None:intergenic | 35.0% |
| ! | TTTGGTTCAAACCTAATTCG+GGG | + | chr4.3:22826159-22826178 | None:intergenic | 35.0% |
| !! | GGAGGCATTTCTCAATTATA+GGG | - | chr4.3:22826585-22826604 | MS.gene052560:CDS | 35.0% |
| !!! | AAGCTGAATGTTGTTGCTTT+TGG | + | chr4.3:22825474-22825493 | None:intergenic | 35.0% |
| !!! | CAATGGGAGAAGATCTTTTT+TGG | - | chr4.3:22825834-22825853 | MS.gene052560:CDS | 35.0% |
| !!! | CTTGTTGCTTTTCTTTGCAA+TGG | + | chr4.3:22825657-22825676 | None:intergenic | 35.0% |
| !!! | GATCTTTTTTGGGCTATAAG+AGG | - | chr4.3:22825845-22825864 | MS.gene052560:CDS | 35.0% |
| ACGATAACCTGTGGAGTTAT+CGG | + | chr4.3:22825923-22825942 | None:intergenic | 40.0% | |
| AGCATTGTTGGAAGAACCAA+AGG | - | chr4.3:22826252-22826271 | MS.gene052560:CDS | 40.0% | |
| AGTAAGGAATTCCCCGAATT+AGG | - | chr4.3:22826145-22826164 | MS.gene052560:CDS | 40.0% | |
| ATGCAATGGAATCCATATGG+TGG | - | chr4.3:22826385-22826404 | MS.gene052560:CDS | 40.0% | |
| CTCGGAGAATTCGAAACATA+AGG | + | chr4.3:22826565-22826584 | None:intergenic | 40.0% | |
| CTGACCATACCAAAAAAGAG+TGG | + | chr4.3:22826212-22826231 | None:intergenic | 40.0% | |
| CTTATGTTTCGAATTCTCCG+AGG | - | chr4.3:22826563-22826582 | MS.gene052560:CDS | 40.0% | |
| GAAATTCTCGGGATCAACTT+TGG | + | chr4.3:22826722-22826741 | None:intergenic | 40.0% | |
| GTGAAAAGGAATGTCAGTGA+AGG | - | chr4.3:22825950-22825969 | MS.gene052560:CDS | 40.0% | |
| GTGATTGTCTTTCAATGCCT+TGG | - | chr4.3:22826179-22826198 | MS.gene052560:CDS | 40.0% | |
| TAATTGAGAAATGCCTCCCT+CGG | + | chr4.3:22826583-22826602 | None:intergenic | 40.0% | |
| TCCTTCATCTTTCCACCATA+TGG | + | chr4.3:22826400-22826419 | None:intergenic | 40.0% | |
| TCTTCCAACAATGCTTCAAG+AGG | + | chr4.3:22826247-22826266 | None:intergenic | 40.0% | |
| ! | TCCATATGGTGGAAAGATGA+AGG | - | chr4.3:22826396-22826415 | MS.gene052560:CDS | 40.0% |
| ! | TTTTGGTATGGTCAGCCAAT+TGG | - | chr4.3:22826217-22826236 | MS.gene052560:CDS | 40.0% |
| !! | GGGAGGCATTTCTCAATTAT+AGG | - | chr4.3:22826584-22826603 | MS.gene052560:CDS | 40.0% |
| !! | TACTCCTCTTGAAGCATTGT+TGG | - | chr4.3:22826240-22826259 | MS.gene052560:CDS | 40.0% |
| !!! | CTTTTTTGGGCTATAAGAGG+TGG | - | chr4.3:22825848-22825867 | MS.gene052560:CDS | 40.0% |
| ACACCGTTTCCTCATAGAGT+AGG | - | chr4.3:22826436-22826455 | MS.gene052560:CDS | 45.0% | |
| AGCAACAACACCGAAACCTT+TGG | - | chr4.3:22825397-22825416 | MS.gene052560:CDS | 45.0% | |
| CAGCTTAGAATTCGAAGTGG+CGG | - | chr4.3:22825488-22825507 | MS.gene052560:CDS | 45.0% | |
| GTTGAATCAGGTGCAACACT+TGG | - | chr4.3:22825617-22825636 | MS.gene052560:CDS | 45.0% | |
| ! | AACAAGCTTGCTTTCCCATC+TGG | - | chr4.3:22825671-22825690 | MS.gene052560:CDS | 45.0% |
| ! | ACAAGCTTGCTTTCCCATCT+GGG | - | chr4.3:22825672-22825691 | MS.gene052560:CDS | 45.0% |
| ! | ATTGGTTCCGATAACTCCAC+AGG | - | chr4.3:22825913-22825932 | MS.gene052560:CDS | 45.0% |
| ! | TCAACAGCATGGGTTGAATC+AGG | - | chr4.3:22825605-22825624 | MS.gene052560:CDS | 45.0% |
| ! | TGAGGAAACGGTGTTTCTGA+TGG | + | chr4.3:22826430-22826449 | None:intergenic | 45.0% |
| ! | TTTTGGGCTATAAGAGGTGG+TGG | - | chr4.3:22825851-22825870 | MS.gene052560:CDS | 45.0% |
| !! | GCTTCAAGAGGAGTACCAAT+TGG | + | chr4.3:22826235-22826254 | None:intergenic | 45.0% |
| AGAGTAAAGCAGACCCCAGA+TGG | + | chr4.3:22825689-22825708 | None:intergenic | 50.0% | |
| GAGTAAAGCAGACCCCAGAT+GGG | + | chr4.3:22825688-22825707 | None:intergenic | 50.0% | |
| GTCTGCTTTACTCTAGGTGC+TGG | - | chr4.3:22825695-22825714 | MS.gene052560:CDS | 50.0% | |
| GTGGTCATTTCTCTGGTGGT+GGG | - | chr4.3:22825717-22825736 | MS.gene052560:CDS | 50.0% | |
| TGTTTCGAATTCTCCGAGGG+AGG | - | chr4.3:22826567-22826586 | MS.gene052560:CDS | 50.0% | |
| ! | CAAGCTTGCTTTCCCATCTG+GGG | - | chr4.3:22825673-22825692 | MS.gene052560:CDS | 50.0% |
| ! | TCTGGGGTCTGCTTTACTCT+AGG | - | chr4.3:22825689-22825708 | MS.gene052560:CDS | 50.0% |
| !! | CATTTCTCTGGTGGTGGGTA+TGG | - | chr4.3:22825722-22825741 | MS.gene052560:CDS | 50.0% |
| !! | TGCTTTACTCTAGGTGCTGG+TGG | - | chr4.3:22825698-22825717 | MS.gene052560:CDS | 50.0% |
| AGTGGCGGTCACGACTTTGA+AGG | - | chr4.3:22825503-22825522 | MS.gene052560:CDS | 55.0% | |
| GCTGGTGGTCATTTCTCTGG+TGG | - | chr4.3:22825713-22825732 | MS.gene052560:CDS | 55.0% | |
| GGTGGTCATTTCTCTGGTGG+TGG | - | chr4.3:22825716-22825735 | MS.gene052560:CDS | 55.0% | |
| ! | TGGGCTATAAGAGGTGGTGG+TGG | - | chr4.3:22825854-22825873 | MS.gene052560:CDS | 55.0% |
| !! | GGTGCTGGTGGTCATTTCTC+TGG | - | chr4.3:22825710-22825729 | MS.gene052560:CDS | 55.0% |
| !!! | GGTGGTGGTGGTGCTAGTTT+TGG | - | chr4.3:22825866-22825885 | MS.gene052560:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 22825368 | 22826792 | 22825368 | ID=MS.gene052560 |
| chr4.3 | mRNA | 22825368 | 22826792 | 22825368 | ID=MS.gene052560.t1;Parent=MS.gene052560 |
| chr4.3 | exon | 22825368 | 22826792 | 22825368 | ID=MS.gene052560.t1.exon1;Parent=MS.gene052560.t1 |
| chr4.3 | CDS | 22825368 | 22826792 | 22825368 | ID=cds.MS.gene052560.t1;Parent=MS.gene052560.t1 |
| Gene Sequence |
| Protein sequence |
