AlfalfaGEDB | MS.gene051341

M. sativa cultivar XinJiangDaYe / MS.gene051341

KEGG: -
GO: -
PFAM: PF00226.34

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051341.t1	XP_013457209.1	92.3	183	8	2	1	181	1	179	4.60E-85	323.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051341.t1	Q9SDN0	37.6	133	71	4	55	178	66	195	2.8e-14	80.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051341.t1	A0A072UZE7	92.3	183	8	2	1	181	1	179	3.3e-85	323.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051341	MS.gene052053	0.804494	2.10E-49	-1.69E-46
MS.gene051341	MS.gene052560	0.809313	2.01E-50	-1.69E-46
MS.gene051341	MS.gene066167	0.801762	7.72E-49	-1.69E-46
MS.gene051341	MS.gene26401	0.830142	3.47E-55	-1.69E-46
MS.gene051341	MS.gene26861	0.834651	2.65E-56	-1.69E-46
MS.gene051341	MS.gene35035	0.969516	3.08E-130	-1.69E-46
MS.gene051341	MS.gene35105	0.805255	1.46E-49	-1.69E-46
MS.gene051341	MS.gene36437	0.802611	5.17E-49	-1.69E-46
MS.gene051341	MS.gene36438	0.803578	3.26E-49	-1.69E-46
MS.gene051341	MS.gene39054	0.81181	5.81E-51	-1.69E-46
MS.gene051341	MS.gene39534	0.816671	4.90E-52	-1.69E-46
MS.gene051341	MS.gene46919	0.804495	2.10E-49	-1.69E-46
MS.gene051341	MS.gene51514	0.825439	4.68E-54	-1.69E-46
MS.gene051341	MS.gene54882	0.801177	1.02E-48	-1.69E-46
MS.gene051341	MS.gene65297	0.814251	1.69E-51	-1.69E-46
MS.gene051341	MS.gene67744	0.850104	2.11E-60	-1.69E-46
MS.gene051341	MS.gene70384	0.919372	4.89E-87	-1.69E-46
MS.gene051341	MS.gene73753	0.820429	6.89E-53	-1.69E-46
MS.gene051341	MS.gene75692	0.810341	1.21E-50	-1.69E-46
MS.gene051341	MS.gene78417	0.801177	1.02E-48	-1.69E-46
MS.gene051341	MS.gene79875	0.812371	4.39E-51	-1.69E-46
MS.gene051341	MS.gene91967	0.800161	1.64E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051341.t1	MTR_4g094275	92.350	183	8	2	1	181	1	179	3.12e-110	312
MS.gene051341.t1	MTR_2g008520	56.522	161	48	6	5	161	6	148	1.85e-46	150
MS.gene051341.t1	MTR_2g008540	56.522	161	48	6	5	161	6	148	1.85e-46	150
MS.gene051341.t1	MTR_8g081620	34.211	190	97	9	9	175	5	189	1.00e-21	87.8
MS.gene051341.t1	MTR_3g061170	42.553	94	48	4	39	128	42	133	1.80e-12	62.4
MS.gene051341.t1	MTR_2g097690	41.026	78	41	2	45	122	65	137	5.27e-12	62.0
MS.gene051341.t1	MTR_2g014470	41.250	80	42	3	53	130	2	78	7.95e-12	62.8
MS.gene051341.t1	MTR_7g085600	33.333	150	88	4	1	146	3	144	1.55e-11	62.0
MS.gene051341.t1	MTR_2g097690	41.026	78	41	2	45	122	65	137	1.78e-11	61.6
MS.gene051341.t1	MTR_5g012530	45.455	66	35	1	56	121	13	77	1.84e-11	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051341.t1	AT4G13830	36.709	158	83	7	31	175	39	192	1.73e-21	87.0
MS.gene051341.t1	AT4G13830	47.222	72	36	2	55	126	66	135	5.30e-14	66.6
MS.gene051341.t1	AT4G13830	47.222	72	36	2	55	126	66	135	7.84e-14	66.6
MS.gene051341.t1	AT3G62600	46.753	77	35	3	53	127	24	96	1.05e-11	62.8
MS.gene051341.t1	AT2G20560	37.500	80	45	3	53	130	2	78	1.95e-11	61.6
MS.gene051341.t1	AT1G28210	40.426	94	49	4	55	147	48	135	3.02e-11	61.6
MS.gene051341.t1	AT1G28210	40.426	94	49	4	55	147	48	135	3.39e-11	61.2
MS.gene051341.t1	AT1G28210	40.426	94	49	4	55	147	48	135	4.52e-11	60.8
MS.gene051341.t1	AT2G22360	33.803	142	71	7	2	135	37	163	4.54e-11	60.8
MS.gene051341.t1	AT4G28480	36.905	84	48	3	53	134	2	82	6.78e-11	60.1
MS.gene051341.t1	AT4G28480	36.905	84	48	3	53	134	2	82	7.99e-11	60.1

Find 28 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAACAATATGATAGTGAATT+AGG	0.232905	4.1:-19466213	MS.gene051341:CDS
GTAGAAATTTGATCCATTCT+TGG	0.275553	4.1:+19466416	None:intergenic
TTTAGTTGATTCCTCTTTCA+TGG	0.279301	4.1:+19466290	None:intergenic
AATGCTTAGGCTTGAGGTTA+AGG	0.338721	4.1:+19466551	None:intergenic
AGTAGACGTATGGGACAAAA+AGG	0.403585	4.1:-19466093	MS.gene051341:CDS
ATATTGTAAATGATGAGATA+TGG	0.436536	4.1:-19466161	MS.gene051341:CDS
AAGTGGTTGAGTTGAAGAAA+AGG	0.458554	4.1:-19466119	MS.gene051341:CDS
ATGATGAGATATGGAGAAGT+AGG	0.468838	4.1:-19466152	MS.gene051341:CDS
AAATTTCTACAAAATGTTGT+CGG	0.490774	4.1:-19466403	MS.gene051341:CDS
AAAGAGAGCTTATAGATCAA+TGG	0.492226	4.1:-19466349	MS.gene051341:CDS
GAAAAGGTCTAGTAGACGTA+TGG	0.496414	4.1:-19466103	MS.gene051341:CDS
GCTTTGAATTTGAAATGCTT+AGG	0.497473	4.1:+19466538	None:intergenic
TCATGATCCTTCCATGAAAG+AGG	0.504521	4.1:-19466301	MS.gene051341:CDS
ATTTGAAATGCTTAGGCTTG+AGG	0.508030	4.1:+19466545	None:intergenic
GTTGATTCCTCTTTCATGGA+AGG	0.513452	4.1:+19466294	None:intergenic
GAATCCGAAAAGCGCAACAA+TGG	0.519653	4.1:-19466379	MS.gene051341:CDS
GAAGGATCATGACACACATC+AGG	0.542719	4.1:+19466312	None:intergenic
CTAGTGGAAGAAACCAAGAA+TGG	0.557604	4.1:-19466429	MS.gene051341:CDS
TCATATTGTTCACGAAGCAT+AGG	0.565520	4.1:+19466225	None:intergenic
ATGTAGAGCAACAAAGCTAG+TGG	0.571091	4.1:-19466445	MS.gene051341:CDS
GGACACAAAGCATGAAAGAT+CGG	0.571745	4.1:-19466047	MS.gene051341:CDS
GGTCTTGGGGTAGCAGAATG+AGG	0.576046	4.1:-19466068	MS.gene051341:CDS
AAAAGGTCTAGTAGACGTAT+GGG	0.582302	4.1:-19466102	MS.gene051341:CDS
AGCTGTTTACTAGAGAGTTG+TGG	0.586525	4.1:+19466501	None:intergenic
AAGTAGGTGGCAAGAACAAG+TGG	0.619538	4.1:-19466136	MS.gene051341:CDS
GTTGCAAAGTACACGTGAGT+AGG	0.624184	4.1:+19466474	None:intergenic
ATGAGATATGGAGAAGTAGG+TGG	0.638622	4.1:-19466149	MS.gene051341:CDS
GTTTACTAGAGAGTTGTGGA+AGG	0.676026	4.1:+19466505	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATCAACTAAAATTTTTGTT+CGG	-	chr4.1:19466327-19466346	MS.gene051341:CDS	15.0%
!!	AAATTTCTACAAAATGTTGT+CGG	-	chr4.1:19466202-19466221	MS.gene051341:CDS	20.0%
!!	ATATTGTAAATGATGAGATA+TGG	-	chr4.1:19466444-19466463	MS.gene051341:CDS	20.0%
!!	GAACAATATGATAGTGAATT+AGG	-	chr4.1:19466392-19466411	MS.gene051341:CDS	25.0%
	AAAGAGAGCTTATAGATCAA+TGG	-	chr4.1:19466256-19466275	MS.gene051341:CDS	30.0%
	GTAGAAATTTGATCCATTCT+TGG	+	chr4.1:19466192-19466211	None:intergenic	30.0%
!	GCTTTGAATTTGAAATGCTT+AGG	+	chr4.1:19466070-19466089	None:intergenic	30.0%
!	TTTAGTTGATTCCTCTTTCA+TGG	+	chr4.1:19466318-19466337	None:intergenic	30.0%
	AAAAGGTCTAGTAGACGTAT+GGG	-	chr4.1:19466503-19466522	MS.gene051341:CDS	35.0%
	ATGATGAGATATGGAGAAGT+AGG	-	chr4.1:19466453-19466472	MS.gene051341:CDS	35.0%
	TCATATTGTTCACGAAGCAT+AGG	+	chr4.1:19466383-19466402	None:intergenic	35.0%
!	ATTTGAAATGCTTAGGCTTG+AGG	+	chr4.1:19466063-19466082	None:intergenic	35.0%
!!	AAGTGGTTGAGTTGAAGAAA+AGG	-	chr4.1:19466486-19466505	MS.gene051341:CDS	35.0%
	AATGCTTAGGCTTGAGGTTA+AGG	+	chr4.1:19466057-19466076	None:intergenic	40.0%
	AGCTGTTTACTAGAGAGTTG+TGG	+	chr4.1:19466107-19466126	None:intergenic	40.0%
	AGTAGACGTATGGGACAAAA+AGG	-	chr4.1:19466512-19466531	MS.gene051341:CDS	40.0%
	ATGAGATATGGAGAAGTAGG+TGG	-	chr4.1:19466456-19466475	MS.gene051341:CDS	40.0%
	ATGTAGAGCAACAAAGCTAG+TGG	-	chr4.1:19466160-19466179	MS.gene051341:CDS	40.0%
	CTAGTGGAAGAAACCAAGAA+TGG	-	chr4.1:19466176-19466195	MS.gene051341:CDS	40.0%
	GAAAAGGTCTAGTAGACGTA+TGG	-	chr4.1:19466502-19466521	MS.gene051341:CDS	40.0%
	GGACACAAAGCATGAAAGAT+CGG	-	chr4.1:19466558-19466577	MS.gene051341:CDS	40.0%
	GTTTACTAGAGAGTTGTGGA+AGG	+	chr4.1:19466103-19466122	None:intergenic	40.0%
	TCATGATCCTTCCATGAAAG+AGG	-	chr4.1:19466304-19466323	MS.gene051341:CDS	40.0%
!	GTTGATTCCTCTTTCATGGA+AGG	+	chr4.1:19466314-19466333	None:intergenic	40.0%
!	TCTTCCATTGTTGCGCTTTT+CGG	+	chr4.1:19466233-19466252	None:intergenic	40.0%
	AAGTAGGTGGCAAGAACAAG+TGG	-	chr4.1:19466469-19466488	MS.gene051341:CDS	45.0%
	GAAGGATCATGACACACATC+AGG	+	chr4.1:19466296-19466315	None:intergenic	45.0%
	GACGTATGGGACAAAAAGGA+AGG	-	chr4.1:19466516-19466535	MS.gene051341:CDS	45.0%
	GGACAAAAAGGAAGGTCTTG+GGG	-	chr4.1:19466524-19466543	MS.gene051341:CDS	45.0%
	GGGACAAAAAGGAAGGTCTT+GGG	-	chr4.1:19466523-19466542	MS.gene051341:CDS	45.0%
	GTTGCAAAGTACACGTGAGT+AGG	+	chr4.1:19466134-19466153	None:intergenic	45.0%
	TGGGACAAAAAGGAAGGTCT+TGG	-	chr4.1:19466522-19466541	MS.gene051341:CDS	45.0%
!	GAATCCGAAAAGCGCAACAA+TGG	-	chr4.1:19466226-19466245	MS.gene051341:CDS	45.0%
	GGTCTTGGGGTAGCAGAATG+AGG	-	chr4.1:19466537-19466556	MS.gene051341:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	19466041	19466586	19466041	ID=MS.gene051341
chr4.1	mRNA	19466041	19466586	19466041	ID=MS.gene051341.t1;Parent=MS.gene051341
chr4.1	exon	19466041	19466586	19466041	ID=MS.gene051341.t1.exon1;Parent=MS.gene051341.t1
chr4.1	CDS	19466041	19466586	19466041	ID=cds.MS.gene051341.t1;Parent=MS.gene051341.t1

Gene Sequence

>MS.gene051341

ATGAATGCTTCATCCTTAACCTCAAGCCTAAGCATTTCAAATTCAAAGCAATTTCATCACCTTCCACAACTCTCTAGTAAACAGCTAAGACCTACTCACGTGTACTTTGCAACAATTTCATGTAGAGCAACAAAGCTAGTGGAAGAAACCAAGAATGGATCAAATTTCTACAAAATGTTGTCGGTGAATCCGAAAAGCGCAACAATGGAAGAAATAAAGAGAGCTTATAGATCAATGGCACTTCAGTATCATCCTGATGTGTGTCATGATCCTTCCATGAAAGAGGAATCAACTAAAATTTTTGTTCGGTTAAATGCAGCTTATGAAACTTTGTCAAATCCTATGCTTCGTGAACAATATGATAGTGAATTAGGTTTGAGAAACAACATGATGAATAATAATAATATTGTAAATGATGAGATATGGAGAAGTAGGTGGCAAGAACAAGTGGTTGAGTTGAAGAAAAGGTCTAGTAGACGTATGGGACAAAAAGGAAGGTCTTGGGGTAGCAGAATGAGGACACAAAGCATGAAAGATCGGAACTAA

Protein sequence

>MS.gene051341.t1

MNASSLTSSLSISNSKQFHHLPQLSSKQLRPTHVYFATISCRATKLVEETKNGSNFYKMLSVNPKSATMEEIKRAYRSMALQYHPDVCHDPSMKEESTKIFVRLNAAYETLSNPMLREQYDSELGLRNNMMNNNNIVNDEIWRSRWQEQVVELKKRSSRRMGQKGRSWGSRMRTQSMKDRN