Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35556.t1 | AES67108.1 | 100 | 55 | 0 | 0 | 1 | 55 | 480 | 534 | 1.90E-18 | 100.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35556.t1 | G7IHH0 | 100.0 | 55 | 0 | 0 | 1 | 55 | 480 | 534 | 1.4e-18 | 100.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052176 | MS.gene35556 | -0.800607 | 1.33E-48 | -1.69E-46 |
| MS.gene052309 | MS.gene35556 | 0.803337 | 3.66E-49 | -1.69E-46 |
| MS.gene053188 | MS.gene35556 | -0.800882 | 1.17E-48 | -1.69E-46 |
| MS.gene057079 | MS.gene35556 | -0.811682 | 6.20E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 5 sgRNAs with CRISPR-Local
Find 4 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAGAAACTGTTAAAGAGA+AGG | 0.542240 | 2.3:+12096270 | MS.gene35556:CDS |
| AACAAGAAAACAAGCAACAT+AGG | 0.548448 | 2.3:-12096142 | None:intergenic |
| TAATCACTTCGCAATTCGAG+TGG | 0.583936 | 2.3:+12096176 | MS.gene35556:CDS |
| TATTAGGAGGAATCATGTCA+AGG | 0.619548 | 2.3:+12096119 | None:intergenic |
| GACTCAACATCAGACACAAG+TGG | 0.644435 | 2.3:-12096205 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACAAGAAAACAAGCAACAT+AGG | - | chr2.3:12096145-12096164 | None:intergenic | 30.0% | |
| TGAAGAAACTGTTAAAGAGA+AGG | + | chr2.3:12096270-12096289 | MS.gene35556:CDS | 30.0% | |
| TAATCACTTCGCAATTCGAG+TGG | + | chr2.3:12096176-12096195 | MS.gene35556:CDS | 40.0% | |
| GACTCAACATCAGACACAAG+TGG | - | chr2.3:12096208-12096227 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 12096125 | 12096291 | 12096125 | ID=MS.gene35556 |
| chr2.3 | mRNA | 12096125 | 12096291 | 12096125 | ID=MS.gene35556.t1;Parent=MS.gene35556 |
| chr2.3 | exon | 12096125 | 12096291 | 12096125 | ID=MS.gene35556.t1.exon1;Parent=MS.gene35556.t1 |
| chr2.3 | CDS | 12096125 | 12096291 | 12096125 | ID=cds.MS.gene35556.t1;Parent=MS.gene35556.t1 |
| Gene Sequence |
| Protein sequence |
