AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene36232

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36232.t1 MTR_2g103370 98.801 834 10 0 1 834 1 834 0.0 1701
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36232.t1 AT2G41620 75.952 840 198 4 1 837 1 839 0.0 1311
MS.gene36232.t1 AT3G57350 73.246 841 218 6 1 837 1 838 0.0 1252

Find 213 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATTCTGGCAATGAGATTT+TGG 0.159071 2.1:+2459326 MS.gene36232:CDS
CGCTCAGTTCCCTCCTCTTC+AGG 0.194338 2.1:+2452095 MS.gene36232:CDS
CAGATTCGGTTGAGGGATTA+TGG 0.218780 2.1:+2456504 MS.gene36232:intron
ACGATGGTTCAAATTTATTT+AGG 0.223167 2.1:-2458138 None:intergenic
AAAGAATGTCATTGATTATT+GGG 0.226836 2.1:+2456153 MS.gene36232:CDS
AAAAGAATGTCATTGATTAT+TGG 0.235409 2.1:+2456152 MS.gene36232:CDS
AGTAGGGGACTTACTTTAAA+TGG 0.241555 2.1:-2455313 None:intergenic
AATAATCAATGACATTCTTT+TGG 0.246454 2.1:-2456150 None:intergenic
GGAATCATGTTGATGCATTA+AGG 0.269660 2.1:+2460023 MS.gene36232:CDS
CCCTGGTCAAATTGTGTCTA+TGG 0.277801 2.1:+2455159 MS.gene36232:CDS
CAAGGGTGCATACGAAAGTT+TGG 0.278688 2.1:+2455903 MS.gene36232:CDS
TGATTATATGATGAAAGTTT+TGG 0.290773 2.1:+2454173 MS.gene36232:CDS
TTCACATCAATTTGCTCCTT+TGG 0.293661 2.1:+2457715 MS.gene36232:CDS
CAGAAGTTTCCACAATTATC+AGG 0.308981 2.1:+2458374 MS.gene36232:CDS
TCCACAATTATCAGGCAATA+TGG 0.312023 2.1:+2458382 MS.gene36232:CDS
TAGACCCATCGCAGATAAAA+AGG 0.313748 2.1:+2455234 MS.gene36232:CDS
AATGCTTAAAAGCAAGATAT+AGG 0.316195 2.1:-2458197 None:intergenic
GGTTATCCTTCTGAGCTTCC+TGG 0.316241 2.1:-2454137 None:intergenic
TCTACTCCACAAGTCTCTTC+TGG 0.319960 2.1:+2455184 MS.gene36232:CDS
ACTTCTGGCCTTATCCATTC+TGG 0.335329 2.1:+2459312 MS.gene36232:CDS
TAATAATTTCACCTGACATC+TGG 0.338858 2.1:-2459366 None:intergenic
GCAGCAGTAGCCACCTCTTC+TGG 0.339368 2.1:-2457848 None:intergenic
CTGCTGTACGCCATCATATC+AGG 0.341394 2.1:+2457944 MS.gene36232:CDS
CGCTTCAGCTCTTACAGTCT+TGG 0.344037 2.1:-2452368 None:intergenic
AAAGAATCCGCGCCTTCCTT+CGG 0.347541 2.1:+2456386 MS.gene36232:CDS
AAAGATATGGCACCTTGTTC+AGG 0.349423 2.1:+2455963 MS.gene36232:CDS
TCGACGCTTGTTGCCTCAAC+AGG 0.349996 2.1:-2453583 None:intergenic
GATGGTGAGAGCCGGACTTC+TGG 0.353288 2.1:+2459297 MS.gene36232:CDS
TGCCGGAGAAGAAGGAGAAT+TGG 0.353697 2.1:+2458609 MS.gene36232:CDS
TCTAACTGAATTATTGTTAA+GGG 0.357730 2.1:+2458552 MS.gene36232:CDS
GTTGAGGGATTATGGGGTTC+TGG 0.358855 2.1:+2456512 MS.gene36232:CDS
TTTAAGCATTCAATTGCTTC+CGG 0.360646 2.1:+2458210 MS.gene36232:CDS
CACTGTTGTTTAGAGGAACT+TGG 0.371971 2.1:+2452312 MS.gene36232:intron
GGCTGTATTATACTTGTCTA+AGG 0.380243 2.1:+2458231 MS.gene36232:CDS
TCGTTCTCAGGTTCATGAAA+TGG 0.382869 2.1:+2454098 MS.gene36232:intron
AAGAATCCGCGCCTTCCTTC+GGG 0.385464 2.1:+2456387 MS.gene36232:CDS
CTTTCTGAAGGCATCAGTAC+TGG 0.386357 2.1:+2458322 MS.gene36232:CDS
TTCTAACTGAATTATTGTTA+AGG 0.393622 2.1:+2458551 MS.gene36232:CDS
TTTGAAGTCATTTGAGTTGA+AGG 0.396716 2.1:+2453049 MS.gene36232:CDS
AAGGGTGCATACGAAAGTTT+GGG 0.396800 2.1:+2455904 MS.gene36232:CDS
AGATTCGGTTGAGGGATTAT+GGG 0.398506 2.1:+2456505 MS.gene36232:intron
TTCAGAGAGGCACTTGTTGA+TGG 0.398841 2.1:-2459244 None:intergenic
CAGTAACGATGCGAAAGATA+TGG 0.400785 2.1:+2455950 MS.gene36232:CDS
TATCTAATGTAGGCTTCTCT+TGG 0.401085 2.1:+2456343 MS.gene36232:intron
GCTGCTGCTGCAGTCGGCGG+TGG 0.402676 2.1:+2458460 MS.gene36232:CDS
GCATGCCAGTGTCAGGAGGC+TGG 0.405039 2.1:+2458682 MS.gene36232:CDS
GCTTCTCTTGGTGGTGGTGT+TGG 0.405273 2.1:+2456355 MS.gene36232:CDS
CTGAATAGAGCAAGGCAGTC+TGG 0.408430 2.1:+2455286 MS.gene36232:CDS
GAAGGTTGCTCTGACTTGTC+TGG 0.416937 2.1:+2460156 MS.gene36232:CDS
ATGCTTAAAAGCAAGATATA+GGG 0.417403 2.1:-2458196 None:intergenic
CTCACAGAGTGGATTAATAC+TGG 0.419658 2.1:+2457815 MS.gene36232:CDS
ATAGCTTGCCGCAACTTTCA+AGG 0.420161 2.1:+2455885 MS.gene36232:intron
TTTCTTGTGGTTCAAATTGT+CGG 0.427428 2.1:+2458030 MS.gene36232:CDS
AATTCACAAATTGTCAAGAC+TGG 0.427511 2.1:+2460000 MS.gene36232:CDS
GGGCAAGGCGCCACCTTGAG+TGG 0.430038 2.1:+2456174 MS.gene36232:CDS
TGCTGCGCAGATTCGGTTGA+GGG 0.430889 2.1:+2456497 MS.gene36232:intron
CTAATGTAGGCTTCTCTTGG+TGG 0.431600 2.1:+2456346 MS.gene36232:intron
GCAAGAGGTGCCGGAGAAGA+AGG 0.439378 2.1:+2458601 MS.gene36232:CDS
TTGTATACCAGACCTCTTGA+AGG 0.447490 2.1:+2460138 MS.gene36232:CDS
AACAGCAATATCAGGTCCTC+GGG 0.447540 2.1:-2460080 None:intergenic
AGCGGCTTGTTCGAGAAGCT+TGG 0.447832 2.1:-2452067 None:intergenic
AACACATCAACAGCAATATC+AGG 0.449707 2.1:-2460088 None:intergenic
CATCAGTACTGGGCAAAAGT+TGG 0.450966 2.1:+2458333 MS.gene36232:CDS
TCCCAATTCTCCTTCTTCTC+CGG 0.453235 2.1:-2458611 None:intergenic
GTGAAGTAGATCGGTCCAAC+TGG 0.454319 2.1:-2452037 None:intergenic
CATGCCAGTGTCAGGAGGCT+GGG 0.455198 2.1:+2458683 MS.gene36232:CDS
TGTTGATACCACATGGCAAC+AGG 0.460280 2.1:+2456569 MS.gene36232:CDS
GCCGGAGAAGAAGGAGAATT+GGG 0.461170 2.1:+2458610 MS.gene36232:CDS
TTACTTGGTGCAAGAGGTGC+CGG 0.461950 2.1:+2458592 MS.gene36232:CDS
GCGTCTTGGTGATCTTCAAA+TGG 0.466910 2.1:+2458417 MS.gene36232:CDS
TTAGCAATCATGTTTGTCCT+TGG 0.468677 2.1:-2455118 None:intergenic
GAATCATGTTGATGCATTAA+GGG 0.468904 2.1:+2460024 MS.gene36232:CDS
AGCAATGTGGGTGCCCGCCC+TGG 0.473304 2.1:+2455142 MS.gene36232:CDS
AAGCTCAGAAGGATAACCTT+AGG 0.474420 2.1:+2454142 MS.gene36232:CDS
CCAAAATCTCATTGCCAGAA+TGG 0.474737 2.1:-2459326 None:intergenic
ATCAGTACTGGGCAAAAGTT+GGG 0.475862 2.1:+2458334 MS.gene36232:CDS
TGCAAATCATCCAAACTGTA+TGG 0.475966 2.1:-2458109 None:intergenic
GTTCAAATTGTCGGCTGTGC+GGG 0.476027 2.1:+2458039 MS.gene36232:CDS
TGAAGCGGCATGCCAGTGTC+AGG 0.479438 2.1:+2458675 MS.gene36232:CDS
GATCGGCTAGCACAATTGAT+AGG 0.481113 2.1:-2458291 None:intergenic
TTGCTGCGCAGATTCGGTTG+AGG 0.481656 2.1:+2456496 MS.gene36232:intron
CAAGCTGCTGCTGCAGTCGG+CGG 0.483082 2.1:+2458457 MS.gene36232:CDS
TCGTCCATTGTTCTAAGTGA+TGG 0.486274 2.1:+2458079 MS.gene36232:CDS
AATGTTGAGAACTGGTGATC+GGG 0.486654 2.1:+2457889 MS.gene36232:CDS
TCTCATTGCCAGAATGGATA+AGG 0.486894 2.1:-2459320 None:intergenic
TGGTCTGATTCCATACAGTT+TGG 0.493704 2.1:+2458099 MS.gene36232:CDS
GGCAATGGTCTCTGCCGTCC+AGG 0.496524 2.1:+2454119 MS.gene36232:CDS
TCAATTGTGCTAGCCGATCA+TGG 0.498941 2.1:+2458295 MS.gene36232:CDS
TGTTGCCATGTGGTATCAAC+AGG 0.498947 2.1:-2456567 None:intergenic
AACAATGGACGAAGATCCAC+TGG 0.500001 2.1:-2458068 None:intergenic
GGTGGACAGTTGTCGTGGAC+TGG 0.502670 2.1:+2458478 MS.gene36232:CDS
AGAGCAACCTTCAAGAGGTC+TGG 0.503916 2.1:-2460145 None:intergenic
CCAAATCTATACTAGGAAAA+GGG 0.505693 2.1:+2460585 MS.gene36232:intron
GGCAAGGCGCCACCTTGAGT+GGG 0.505759 2.1:+2456175 MS.gene36232:CDS
TGCGATGGGTCTACTAGTCA+TGG 0.505854 2.1:-2455224 None:intergenic
GAATAACACATTACTTGTGC+GGG 0.509007 2.1:-2456236 None:intergenic
AATGGATAAGGCCAGAAGTC+CGG 0.510606 2.1:-2459308 None:intergenic
TGTGAAAGAATGTTGAGAAC+TGG 0.510947 2.1:+2457881 MS.gene36232:CDS
TGGGCAAAAGTTGGGAGTGA+TGG 0.514092 2.1:+2458342 MS.gene36232:CDS
AATAATTTCACCTGACATCT+GGG 0.517279 2.1:-2459365 None:intergenic
TCTAAGGAAGCAGGAGATGA+AGG 0.518714 2.1:+2458247 MS.gene36232:CDS
CAGTTGTCGTGGACTGGTAG+AGG 0.522609 2.1:+2458484 MS.gene36232:CDS
CAACAGCAATATCAGGTCCT+CGG 0.524800 2.1:-2460081 None:intergenic
GGCTAGCACAATTGATAGGT+GGG 0.527583 2.1:-2458287 None:intergenic
GGATCTATGTATTTGCGTCT+TGG 0.529918 2.1:+2458403 MS.gene36232:CDS
ACTGAATTATTGTTAAGGGA+TGG 0.530872 2.1:+2458556 MS.gene36232:CDS
ATTTCCTTCAGAGTTTAAGC+AGG 0.532379 2.1:+2455083 MS.gene36232:CDS
TTATACTTGTCTAAGGAAGC+AGG 0.533407 2.1:+2458238 MS.gene36232:CDS
GGCTTGTTCGAGAAGCTTGG+AGG 0.534973 2.1:-2452064 None:intergenic
AAATCCTGCTTAAACTCTGA+AGG 0.537989 2.1:-2455087 None:intergenic
ATACTTACTACCCAGATGTC+AGG 0.538443 2.1:+2459355 MS.gene36232:CDS
ATAGAAATTCAGAAGAGAGT+TGG 0.538979 2.1:+2459198 MS.gene36232:CDS
ATTCACAAATTGTCAAGACT+GGG 0.541007 2.1:+2460001 MS.gene36232:CDS
TGCCGTCCAGGAAGCTCAGA+AGG 0.542467 2.1:+2454131 MS.gene36232:CDS
GGATAAATCTTCGTTCGCCA+TGG 0.543632 2.1:-2452016 None:intergenic
AGACACAATTTGACCAGGGC+GGG 0.546301 2.1:-2455155 None:intergenic
AGGGAACTGAGCGGAAGGAG+CGG 0.547144 2.1:-2452085 None:intergenic
TCTTCGAGAACCTGATATGA+TGG 0.547163 2.1:-2457954 None:intergenic
TTCAGAAAGGACGCCATGAT+CGG 0.547176 2.1:-2458308 None:intergenic
GAATGTTGAGAACTGGTGAT+CGG 0.548567 2.1:+2457888 MS.gene36232:CDS
AGAGGTCTGGTATACAAGCT+TGG 0.551386 2.1:-2460132 None:intergenic
GAGTTGGTTGATCTCTAAGC+AGG 0.552770 2.1:-2457988 None:intergenic
GCACAAGCTGCTGCTGCAGT+CGG 0.553605 2.1:+2458454 MS.gene36232:CDS
TATAATCATTGAAACTCCTA+AGG 0.553720 2.1:-2454158 None:intergenic
ATGTAGGCTTCTCTTGGTGG+TGG 0.555114 2.1:+2456349 MS.gene36232:intron
GAAACAGATGCTGATGGATG+AGG 0.555847 2.1:+2456106 MS.gene36232:intron
CGTCGAAGAGTATCTGCAGC+AGG 0.556391 2.1:+2453600 MS.gene36232:CDS
TTTCTGAAGGCATCAGTACT+GGG 0.556554 2.1:+2458323 MS.gene36232:CDS
TAAATGCAGAGCAGCTTGCA+AGG 0.557101 2.1:+2453024 MS.gene36232:CDS
AAGAATGTCATTGATTATTG+GGG 0.558574 2.1:+2456154 MS.gene36232:CDS
TAGCTTGCCGCAACTTTCAA+GGG 0.559302 2.1:+2455886 MS.gene36232:intron
TCCATATTGCCTGATAATTG+TGG 0.560589 2.1:-2458383 None:intergenic
GGTATCAAGCGCCACTGAGA+AGG 0.560618 2.1:-2459223 None:intergenic
GTGGATTAATACTGGAGGAA+TGG 0.561929 2.1:+2457823 MS.gene36232:CDS
GTATGCACCCTTGAAAGTTG+CGG 0.563942 2.1:-2455893 None:intergenic
ACAAGTCTCTTCTGGTAGCA+TGG 0.566207 2.1:+2455192 MS.gene36232:CDS
AGAGTACCTAGTTGCAGCAA+TGG 0.566795 2.1:-2452401 None:intergenic
GAATTATTGTTAAGGGATGG+AGG 0.568335 2.1:+2458559 MS.gene36232:CDS
TGTCATTGATTATTGGGGCA+AGG 0.568514 2.1:+2456159 MS.gene36232:CDS
GCAAGATATAGGGATATACC+AGG 0.570594 2.1:-2458186 None:intergenic
CGGCTAGCACAATTGATAGG+TGG 0.571111 2.1:-2458288 None:intergenic
ACTTCGTGCTTTGAGAGCTA+AGG 0.571432 2.1:+2460204 MS.gene36232:CDS
ATTGAGAGAACCTGAAGAGG+AGG 0.572086 2.1:-2452105 None:intergenic
TCTCACCATCTAACCTTCCA+CGG 0.572182 2.1:-2459284 None:intergenic
ATTTCTCATGTCCCCACTCA+AGG 0.575810 2.1:-2456187 None:intergenic
GAACTGGTGATCGGGTAGGC+CGG 0.576829 2.1:+2457897 MS.gene36232:CDS
TTAGACAAGTATAATACAGC+CGG 0.577630 2.1:-2458229 None:intergenic
GGTATACAAGCTTGGACATG+AGG 0.577743 2.1:-2460124 None:intergenic
TGGAGGAATGGTGCCAGAAG+AGG 0.578745 2.1:+2457835 MS.gene36232:CDS
GATTCGGTTGAGGGATTATG+GGG 0.578836 2.1:+2456506 MS.gene36232:intron
TCGGCGGTGGACAGTTGTCG+TGG 0.580039 2.1:+2458473 MS.gene36232:CDS
ATCCTTCTGAGCTTCCTGGA+CGG 0.581007 2.1:-2454133 None:intergenic
GACAATGTGACAGACTCTGA+TGG 0.582532 2.1:+2460178 MS.gene36232:CDS
ACCATAGACACAATTTGACC+AGG 0.583473 2.1:-2455160 None:intergenic
TGAATAACACATTACTTGTG+CGG 0.585399 2.1:-2456237 None:intergenic
ACATCCCAGCCTCCTGACAC+TGG 0.585632 2.1:-2458687 None:intergenic
ATACAGGCTTCTTGCTCGTG+AGG 0.588254 2.1:+2452998 MS.gene36232:intron
CAGTACTGATGCCTTCAGAA+AGG 0.591264 2.1:-2458321 None:intergenic
GATCATGGCGTCCTTTCTGA+AGG 0.591595 2.1:+2458310 MS.gene36232:CDS
CGAACGAAGATTTATCCAGT+TGG 0.592391 2.1:+2452022 MS.gene36232:CDS
GAAGGTTAGATGGTGAGAGC+CGG 0.594461 2.1:+2459289 MS.gene36232:CDS
CACAGTCCATTGCTGCAACT+AGG 0.595342 2.1:+2452395 MS.gene36232:CDS
TACAGGCTTCTTGCTCGTGA+GGG 0.595779 2.1:+2452999 MS.gene36232:intron
GCAAGGCGCCACCTTGAGTG+GGG 0.595896 2.1:+2456176 MS.gene36232:CDS
CTAATTGAAACAGATGCTGA+TGG 0.595941 2.1:+2456100 MS.gene36232:intron
GAGATTGAGAGAACCTGAAG+AGG 0.598963 2.1:-2452108 None:intergenic
GAGGTAATGTTGATCAACAA+AGG 0.600325 2.1:+2458503 MS.gene36232:CDS
GGGACATGAGAAATACATCA+TGG 0.600464 2.1:+2456196 MS.gene36232:CDS
AGGAATGGTGCCAGAAGAGG+TGG 0.600952 2.1:+2457838 MS.gene36232:CDS
AAGTCAGAGCAACCTTCAAG+AGG 0.601145 2.1:-2460150 None:intergenic
GCGATGGGTCTACTAGTCAT+GGG 0.603772 2.1:-2455223 None:intergenic
GTTGAGACGAACCCGAAGGA+AGG 0.608990 2.1:-2456398 None:intergenic
GGAGGAAGAGTGAAGTAGAT+CGG 0.609858 2.1:-2452046 None:intergenic
AGCGGCATGCCAGTGTCAGG+AGG 0.613053 2.1:+2458678 MS.gene36232:CDS
AGAGGAGGGAACTGAGCGGA+AGG 0.613962 2.1:-2452090 None:intergenic
TCAGGTTCATGAAATGGCAA+TGG 0.615202 2.1:+2454104 MS.gene36232:intron
CAGACCATCACTTAGAACAA+TGG 0.617186 2.1:-2458083 None:intergenic
CAACAAGTACAACCTGAACA+AGG 0.624193 2.1:-2455975 None:intergenic
AATCTATACTAGGAAAAGGG+TGG 0.626456 2.1:+2460588 MS.gene36232:intron
TGCCATGTGGTATCAACAGG+TGG 0.627027 2.1:-2456564 None:intergenic
ATGATTGCTAATAGCAATGT+GGG 0.627930 2.1:+2455130 MS.gene36232:CDS
TTGGGCGTTGATGCTTGTGG+TGG 0.630503 2.1:+2455922 MS.gene36232:CDS
TTGAGAACTGGTGATCGGGT+AGG 0.632782 2.1:+2457893 MS.gene36232:CDS
TTGTAGCGGATCCAAATGCA+AGG 0.634980 2.1:+2458638 MS.gene36232:CDS
CAAGATATAGGGATATACCA+GGG 0.636735 2.1:-2458185 None:intergenic
TCAGGTCCTCGGGGATCTAG+AGG 0.636836 2.1:-2460070 None:intergenic
GCTGTGCGGGACTGCCCCAG+TGG 0.639313 2.1:+2458052 MS.gene36232:CDS
TTGAGAGAACCTGAAGAGGA+GGG 0.639596 2.1:-2452104 None:intergenic
GCACGATAGAGGATTTCTTG+TGG 0.640475 2.1:+2458017 MS.gene36232:CDS
GCAGGATTTCAACATTACCA+AGG 0.642066 2.1:+2455101 MS.gene36232:CDS
GGTTCAAATTGTCGGCTGTG+CGG 0.644450 2.1:+2458038 MS.gene36232:CDS
GTTGCAGCAATGGACTGTGA+CGG 0.646464 2.1:-2452391 None:intergenic
GACGAACCCGAAGGAAGGCG+CGG 0.646810 2.1:-2456393 None:intergenic
AAATGCAGAGCAGCTTGCAA+GGG 0.647635 2.1:+2453025 MS.gene36232:CDS
TCATGTTGATGCATTAAGGG+AGG 0.649430 2.1:+2460027 MS.gene36232:CDS
ATGCTACCAGAAGAGACTTG+TGG 0.653180 2.1:-2455190 None:intergenic
AGTTTGGGCGTTGATGCTTG+TGG 0.654514 2.1:+2455919 MS.gene36232:CDS
CATGATTGCTAATAGCAATG+TGG 0.654831 2.1:+2455129 MS.gene36232:CDS
GAGAGTTGGTTCCTTCTCAG+TGG 0.668521 2.1:+2459212 MS.gene36232:CDS
AGCCACCTGTTGATACCACA+TGG 0.668750 2.1:+2456562 MS.gene36232:CDS
GTTTCTTACAGCTCACAGAG+TGG 0.670484 2.1:+2457804 MS.gene36232:intron
CCATAGACACAATTTGACCA+GGG 0.670943 2.1:-2455159 None:intergenic
TCTCATGTCCCCACTCAAGG+TGG 0.676167 2.1:-2456184 None:intergenic
TTCACAAATTGTCAAGACTG+GGG 0.679733 2.1:+2460002 MS.gene36232:CDS
AAGATATAGGGATATACCAG+GGG 0.683071 2.1:-2458184 None:intergenic
ACAATGGACGAAGATCCACT+GGG 0.689644 2.1:-2458067 None:intergenic
ACAGAGTGGATTAATACTGG+AGG 0.690461 2.1:+2457818 MS.gene36232:CDS
TAGACACAATTTGACCAGGG+CGG 0.691604 2.1:-2455156 None:intergenic
GAGAACAAATAGCTTCAGAG+AGG 0.705513 2.1:-2459257 None:intergenic
CTGAAGAGGAGGGAACTGAG+CGG 0.706436 2.1:-2452094 None:intergenic
AGTCAAGACCTGTTGCCATG+TGG 0.718128 2.1:-2456577 None:intergenic
ACAGCAATATCAGGTCCTCG+GGG 0.757630 2.1:-2460079 None:intergenic
CAATGGACGAAGATCCACTG+GGG 0.814652 2.1:-2458066 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr2.1 gene 2452018 2460632 2452018 ID=MS.gene36232
chr2.1 mRNA 2452018 2460632 2452018 ID=MS.gene36232.t1;Parent=MS.gene36232
chr2.1 exon 2452018 2452116 2452018 ID=MS.gene36232.t1.exon1;Parent=MS.gene36232.t1
chr2.1 CDS 2452018 2452116 2452018 ID=cds.MS.gene36232.t1;Parent=MS.gene36232.t1
chr2.1 exon 2452325 2452416 2452325 ID=MS.gene36232.t1.exon2;Parent=MS.gene36232.t1
chr2.1 CDS 2452325 2452416 2452325 ID=cds.MS.gene36232.t1;Parent=MS.gene36232.t1
chr2.1 exon 2453004 2453070 2453004 ID=MS.gene36232.t1.exon3;Parent=MS.gene36232.t1
chr2.1 CDS 2453004 2453070 2453004 ID=cds.MS.gene36232.t1;Parent=MS.gene36232.t1
chr2.1 exon 2453562 2453621 2453562 ID=MS.gene36232.t1.exon4;Parent=MS.gene36232.t1
chr2.1 CDS 2453562 2453621 2453562 ID=cds.MS.gene36232.t1;Parent=MS.gene36232.t1
chr2.1 exon 2454108 2454197 2454108 ID=MS.gene36232.t1.exon5;Parent=MS.gene36232.t1
chr2.1 CDS 2454108 2454197 2454108 ID=cds.MS.gene36232.t1;Parent=MS.gene36232.t1
chr2.1 exon 2455058 2455321 2455058 ID=MS.gene36232.t1.exon6;Parent=MS.gene36232.t1
chr2.1 CDS 2455058 2455321 2455058 ID=cds.MS.gene36232.t1;Parent=MS.gene36232.t1
chr2.1 exon 2455889 2455984 2455889 ID=MS.gene36232.t1.exon7;Parent=MS.gene36232.t1
chr2.1 CDS 2455889 2455984 2455889 ID=cds.MS.gene36232.t1;Parent=MS.gene36232.t1
chr2.1 exon 2456113 2456244 2456113 ID=MS.gene36232.t1.exon8;Parent=MS.gene36232.t1
chr2.1 CDS 2456113 2456244 2456113 ID=cds.MS.gene36232.t1;Parent=MS.gene36232.t1
chr2.1 exon 2456355 2456408 2456355 ID=MS.gene36232.t1.exon9;Parent=MS.gene36232.t1
chr2.1 CDS 2456355 2456408 2456355 ID=cds.MS.gene36232.t1;Parent=MS.gene36232.t1
chr2.1 exon 2456507 2456590 2456507 ID=MS.gene36232.t1.exon10;Parent=MS.gene36232.t1
chr2.1 CDS 2456507 2456590 2456507 ID=cds.MS.gene36232.t1;Parent=MS.gene36232.t1
chr2.1 exon 2457650 2457736 2457650 ID=MS.gene36232.t1.exon11;Parent=MS.gene36232.t1
chr2.1 CDS 2457650 2457736 2457650 ID=cds.MS.gene36232.t1;Parent=MS.gene36232.t1
chr2.1 exon 2457815 2458717 2457815 ID=MS.gene36232.t1.exon12;Parent=MS.gene36232.t1
chr2.1 CDS 2457815 2458717 2457815 ID=cds.MS.gene36232.t1;Parent=MS.gene36232.t1
chr2.1 exon 2459195 2459376 2459195 ID=MS.gene36232.t1.exon13;Parent=MS.gene36232.t1
chr2.1 CDS 2459195 2459376 2459195 ID=cds.MS.gene36232.t1;Parent=MS.gene36232.t1
chr2.1 exon 2459934 2460225 2459934 ID=MS.gene36232.t1.exon14;Parent=MS.gene36232.t1
chr2.1 CDS 2459934 2460225 2459934 ID=cds.MS.gene36232.t1;Parent=MS.gene36232.t1
chr2.1 exon 2460600 2460632 2460600 ID=MS.gene36232.t1.exon15;Parent=MS.gene36232.t1
chr2.1 CDS 2460600 2460632 2460600 ID=cds.MS.gene36232.t1;Parent=MS.gene36232.t1
Gene Sequence

>MS.gene36232

ATGGCGAACGAAGATTTATCCAGTTGGACCGATCTACTTCACTCTTCCTCCAAGCTTCTCGAACAAGCCGCTCCTTCCGCTCAGTTCCCTCCTCTTCAGGTTCTCTCAATCTCACCATTTTTTCACTCTTCTTTTTTTAGGTTAAGTTTATATGCATTTTGGTTTTCAACTATGCTTAGCTTCAATTCGATTGAATTATTGAATAATTATTACTTTCTGTTAGTTAGAATTGTTTTTGACTGAAAATTGAAATAATTATTGATTTTTACTATGATTTTGGGGATTTATTATTTTCACTGTTGTTTAGAGGAACTTGGATCAACTTGAAGCTTTATCGAAGAAGCTTAAATCCAAGACTGTAAGAGCTGAAGCGCCGTCACAGTCCATTGCTGCAACTAGGTACTCTATAGTTCTTGTTAATGCGAAATTTATATTCTGTTCATAAGTTGTTCTATTATGTTTTGGTAAGTTTCTAGTTTAGTTGGATTGCTTTTAGTGCATGTTTGGTTTTGCCGTGACAAAAATTGATTTTGTAAAATTGATTTTACTTAAAAGTGAGTTGAAGGATTGTAAAAGGATTTATGTTTGGTTGGATACATTTAGGTAAAAGTGAGTTGAAAAATAAATTTCAGTTAAACAACACGTTTAGAATTAAAGCTAAAAATCCTGGATTTAAGATAGAATCAATTATGGAGGCAAAATCAATTTTACTCAAGAGCAACCAAACATGTCAAAATCAATTTTACACTTTAGAATCAATTTCTTTGATTATGATACATTATTATTCTTTCAAATGCTTTTTTTGCTTTCTCAGTAGGGGCATTGCTTTTGTTTTACATATTTCTAATAGGGCATGTGGAGCCATCATACATGTCCTAATAAACTGCCTTCCATCATTTTCTTAAAGCTAAAGTTACTTGAATTCACGACAGCATATAGGCAATTAGTGTAATAAAATCTGTTATTTATTTTGCACTCACATACAGGCTTCTTGCTCGTGAGGGTATAAATGCAGAGCAGCTTGCAAGGGATTTGAAGTCATTTGAGTTGAAGGTACCACTTAACATATATTTTGCAATCATTTTTACATTTAACATCTTATTGTTGTTGCTTTGCGTACCATGTGCGTCTTGTGCGGGTATTGCTTAATAAAATTTACCGTTTCAAAAAAAAAAAAAAACATCTTTACCTTTTCTTGGATTGTACTATTCTATTTATTTGCCCTTACACTATAATTTATGCACAACAAACTAGTTTTCATTATTTTTGAGCTGCCATACTTAAAGTTGCACAAACTTCCTTGTTTTCATGATATAACCTATTTGGATAGAATTCCTTGTATAAACTTTCCTGATGTAACCATACTTTAAGTTGTATAGACATCCTTGTTTTCCTGATATAACCTAACTGGATGTAAACTTAATTTTGTTGGAAGTTGACGTCAAATTACAAGCTTAGTTGCTCTTGTTGGGATTTAAATTCTTCAACTCATATATTTTTTTGCTAATGCTGTGCCCTTTGCCTGTTTTTGTTGTTTTGTAAAGACAACTTTTGAGGATGTTTTTCCTGTTGAGGCAACAAGCGTCGAAGAGTATCTGCAGCAGGTAATTTCTACTTTCAAATTGCTTGTTATCCATCTTGGATTTAGTTATCACTTTAAAATCATTATTAGACATCAGTTTGATTCAATCTGAATAGTTTTTATGCGTGTTAGATATCATGTACTCTAAGTCTACCTTTTTGAGTTTGCTTTCATTTTTCTCCAGCAAAAATGTTCACAAAAATCAATGAAAACTATAATGTCGGAGTTCTGAATAGGATGTTTGATTTTTTCAATAAATCACTTGTCAAAGTTAAAGAGCTGTCCTCTGAACTATCTTAAAAAAAAAAGAGCTGTCCTTTGTTGATGTGCGTGGTATTGTAGCATGTTCCCCCTTATTTTTTATGAGCATAGCAAAATTAGTTCAGTGTTTGTATGTGAAGTTATGTACCTTGCTATGGGAAACCAATTAAGTCTCCTTACTCTTCACTTGACTTGGTCCTTTCTGAATAGAATTGTGACAATATTGAATACTCTTTTTCGTTCTCAGGTTCATGAAATGGCAATGGTCTCTGCCGTCCAGGAAGCTCAGAAGGATAACCTTAGGAGTTTCAATGATTATATGATGAAAGTTTTGGAGGTTAGTGTAATCTTGCCTTTCATGTCATGGCACACCATGATTTTTTCTGTATTCTTTGTGTAATTATTGGTTATTACTGGAGAAATTTTTGTAATGTGTTTTGCTAAGCTATTTATAAAATAACAGTTATTGGGGTAAGATTTGGTAAACCATTAATTTGGATACTCAATGTGTCCTGTCTTTTTCTTGCTTTGCTGAAAATAAGATATCTTATGGATGTGCCTATTTTATTTTCTTTATTTATTTGATTGTAGTCAAAAGCCTTAAACTTTCAAAAGGAAATATCAGTCCTTATATTTTTGTGTGGATATGGGTACAAGATTATGACAAAGGAATTGATGGGATTTATCGAACTATAATAAGGTTGGTTGTTTTTGCATACTTTTTGATGAGGATATATCCTTTTTCGGTGAACCTTGTCTGTAATCCTTGTTTCCTCTGTGTATGAAAATTTCTCACATTCTTTTAGAAAATCCAATATTAAAAATGAATAGAAAACAAAATGTGTAAAGCGTAGTTTTTGTTTAATTTATACAACTCGATAGTTTTCCATAATTTTGACTTTGGCAATGAGGTTTGCTGACCTACTTAAACTTCTCACCAATCAAATCATCCCATTTGGCTGATTGATTGTTTTTCTATAAAATATGCTTAGTTTAACATGTGGAACTTCAATATGAAATGAAGTAGCATAGTGAAGTAAGTAAATCACTATATAATATATATCATATTTGCAATTTTATAATTGGGATGAGTATTTTATTGCATTGTTATATTGGTCATAAAAATATGTTTATTTGTTCTTGATATTATGTGGCTCATCCTTCTTACTGCTCAGTAGCATTTAATGTTTTTGACAGGAGGATTGGCAAAAAGAAAAGCGTGATTTCCTTCAGAGTTTAAGCAGGATTTCAACATTACCAAGGACAAACATGATTGCTAATAGCAATGTGGGTGCCCGCCCTGGTCAAATTGTGTCTATGGTTTCTACTCCACAAGTCTCTTCTGGTAGCATGGTGATTGTTCCCATGACTAGTAGACCCATCGCAGATAAAAAGGCATCTGTCTATGCTGAAGTAGTTAAAAATCTGAATAGAGCAAGGCAGTCTGGATTGCCATTTAAAGTAAGTCCCCTACTTTCAGAGCTCTATATTAGATCAAAAACCTAGTAGATGGTGTAGCCTGTTCATGACTTCAGTGATCGTGTTTGGAACCCAAATTCAAGAGAAATAGAAATTGTATTATTGATTGGAATAAGGAGAACTATTACAGAGAATGATCCCTATACTCCCAGAGGAAAAGCTCCTCAGAGAAGAGACATTTCTCTCTCTCTGCCCAAATTACAAGATTCTAACTAAATAATGGGAATACTCCTCTCAATTGCTATCCCTATCCTTTATATAACTAACATTTCTCTAACAAACTAGCCCCCTCACCCAATGTAACTAACTCTCCTAGTAAGCACATTAGTTGGGCTTGAGCCCAAACTCAGTACCTAACAGATCAACATTGTGATTTGCATGAAGCTAATGTATAAACTTTTAATTCAATACTGTTCCAACATCATTTTGAACATAAGCAATGCTTTCTTGTTTTTGAGATCCTTTCCCAAAAGTTCTTACTAGTATTCACCTTTTTGTATTTATATGTATGTACGCTAATTTTCATAATAATTTATCTATGCTTGATAGCTTGCCGCAACTTTCAAGGGTGCATACGAAAGTTTGGGCGTTGATGCTTGTGGTGGAAAATCAGTAACGATGCGAAAGATATGGCACCTTGTTCAGGTTGTACTTGTTGTTGTATACTGTTAACAGGCAAAACAGAGAGAAACTTTTGCCTGCAACTTTATTGGTTCTACCTGTTATGAAGTAAACATTTTTATTTATTTTCTAGACTTGGCTAATTGAAACAGATGCTGATGGATGAGGACTCAACCTTGCGACGTGTTTCCAAAAGAATGTCATTGATTATTGGGGCAAGGCGCCACCTTGAGTGGGGACATGAGAAATACATCATGGATACTATACATAATCATCCCGCACAAGTAATGTGTTATTCAGAATTTCAAGTGGTTTATTCATATGAGAGCATGCATTGATTTTTATTTGTTGTATCAACACTTAATTTCTGACATCTTTCTTTTATCTAATGTAGGCTTCTCTTGGTGGTGGTGTTGGAAACTTGCAAAGAATCCGCGCCTTCCTTCGGGTTCGTCTCAACTTGAAGAATATGCGTCTTGTTAAACACTTTACCATGTATTTAATTTTTTTTTCCTTCAAAAAATCCCTGATTTTATTGCTGCGCAGATTCGGTTGAGGGATTATGGGGTTCTGGATTTTGATGCTGGTGATGCTCGGAGACAGCCACCTGTTGATACCACATGGCAACAGGTCTTGACTACTTTATCTTACTATAAAATTATTAGGGACGAGACTTGAAATTGAATCCGAAGGGTGCATACAAATTACTCGCCATGAGTACGATTTTTAGTCATCAAATGGATTTGGGTCTAGGCATGGGTAATACTAATACATATAAAAAATTGTGGGCATTACCATGGGGCATGGGGGCCATATGTTTTATTATATTTTATCCAAACGATATGCTTCATATATTTTATAACTAGCCGTAACAGTAATACCCATGCTTTGTATATGTTACTTTTGAATGGAGTAAATTCCGTTTTTTTATTATATGTTAATCATCTTATAAAGAATTTAGAGTGAAAGAAAAAAAGGTTCCGGGCCTCCAAGAGTATGTGAAAAAATCTTTAATGCAAAAAAGTATCTATAAAAACTACGGGTATAAGTATCTAAACCTACATCTTACCTGCACAATATGCTCATCATATTTTATAACAACACGTTATACCCTTCCTTTGTATGGGTTACTTATAAATGAAACAAGAATTCTGTTTAAAAAAGGGCAACCCGGTGCACTAAAGCTCCCACATACGCAGGGTCCGGGAAGGGAAGTCGTGGTACTGTTGGGATATGTAGACAGATTTATAGAACTCTATAGATTAGGATAGGATTTCAAATATCTCTGGATATTTTTTAATGAAGTTAGATTTTACACTTGTCAATGCTATTTTTGTTGACTTATCATATACTCAAATAAATTTACCAGTATCTTAAAAGAAAAAGATACATCAATGGAAACAAATCCCATAACCACAACATGAGTTTTGTTTGCTTTCATATCCGAGTATATAGGCATCTATTAGGTACGGTTAAGGGCCTCCAAGTTGCTACCCGCATGAGTTTGGGTTTGTGGCAATTTCTTAAATGTGGATATGGATATTCTAGTTTTTTACATAGACTCCACGCTTTGCTATCCCTACAATTTAGTTACACTTCATTAACAGAGTTCAGTCCTATATCTAATTTTTTTGTTGCTTAAAATCAATATTAATGATGAGCTAAGCTTGTACTTTCTTTTTTTAACAGATATATTTTTGTCTGAGGTCTGGTTATTACGATGAAGCAAGAAATGTTGCTCTATCTTCGCGTGCTTCACATCAATTTGCTCCTTTGGTACATATACATAGTGTGATCACTTCTTGTTATGTGCTTATTTATAGTTCACTTTTAATAATTTACTGTTTCTTACAGCTCACAGAGTGGATTAATACTGGAGGAATGGTGCCAGAAGAGGTGGCTACTGCTGCATCAGAAGAATGTGAAAGAATGTTGAGAACTGGTGATCGGGTAGGCCGGACTGCATACGACAAGAAAAAATTACTGCTGTACGCCATCATATCAGGTTCTCGAAGACATATTGATCGCCTGCTTAGAGATCAACCAACTCTTTTTAGCACGATAGAGGATTTCTTGTGGTTCAAATTGTCGGCTGTGCGGGACTGCCCCAGTGGATCTTCGTCCATTGTTCTAAGTGATGGTCTGATTCCATACAGTTTGGATGATTTGCAAAGTTACCTAAATAAATTTGAACCATCGTACTATACAAAAAATGGAAAAGATCCCCTGGTATATCCCTATATCTTGCTTTTAAGCATTCAATTGCTTCCGGCTGTATTATACTTGTCTAAGGAAGCAGGAGATGAAGGATATAACATTGATGCTGCCCACCTATCAATTGTGCTAGCCGATCATGGCGTCCTTTCTGAAGGCATCAGTACTGGGCAAAAGTTGGGAGTGATGGATGCTTATGCAGAAGTTTCCACAATTATCAGGCAATATGGATCTATGTATTTGCGTCTTGGTGATCTTCAAATGGCACTAGAATATTATGCACAAGCTGCTGCTGCAGTCGGCGGTGGACAGTTGTCGTGGACTGGTAGAGGTAATGTTGATCAACAAAGGCAGAGAAACTTGATGCTGAAGCAGCTTCTAACTGAATTATTGTTAAGGGATGGAGGTATATATCTTTTACTTGGTGCAAGAGGTGCCGGAGAAGAAGGAGAATTGGGACGTTTTGTAGCGGATCCAAATGCAAGGCAACAATTTTTAATTGAAGCGGCATGCCAGTGTCAGGAGGCTGGGATGTATGACAAAGTAATTTTTTTCTCATGCTACTCTTTTATTTTAGAGGAACAATAGGGAAAGTAAGAAAACCTTCAACTGCTTTTACTGAATTACAACATTACCTAGCTCACAATCATTGTTCTTCATGCCCTTATTGCGGCACTTAAATATGGAAATTACTCCACTCAGTTTTTAGACATTTGTTTTTGCCTCTTAGACATCCTTTTTCTGGTGAAGCTAAAGATATTTTTAACACCATCTAGGAATTTTGTTAACTCTTGTTTATTTTCAATTTCTAATACCTTTTTGATTACATTTCATTATTTCTCACATTTGTGTCTCTCATACTGTGTCTCTACTCTCTAGATTTTTCCCACCTAGGTTATTTAAACTTATTTTCTCCTAATTTTTGTTACCTCCGTGTTCCCTTATTCATCACCATTTGTGAGTAAGTTTTTTCATCCAACCTTTATGTTCCTTTTGCTGATGCGGAATTTTTTGGTTCAGTCTATAGAAATTCAGAAGAGAGTTGGTTCCTTCTCAGTGGCGCTTGATACCATCAACAAGTGCCTCTCTGAAGCTATTTGTTCTCTTTTCCGTGGAAGGTTAGATGGTGAGAGCCGGACTTCTGGCCTTATCCATTCTGGCAATGAGATTTTGGAGACATATACTTACTACCCAGATGTCAGGTGAAATTATTACCTTTTATTTCCTTGCTCACATATTTCTCTGCTTGGACTTTGGGATTGTGATATTCTTAAACTTTGCATGATATTTAAAAAATGAACCTTTTCAAGTTGTGGTGATTTGACACATATTATGCTTTGGTTGTTATTTTCATCTTTTCCCTTATCTTTTTTCATGGAATGATCTTTGAGGTCAAACCCTGCTTGTGTGTAACTTGGAAGTCCATTTTCGCCATATCCTTACTTCACATGGCTATTGGTGGTAATTTACATGCAAAAACAGGTGGCACAAAGATGAATGACGGGTGATATGGGATATACCATTCACAGGATTACGCCATCTTTTTGCTGATACTACGGTTGTAGTCTTTGTTAGGTGTTGGTTTTGTTTTGTTTTTTCTGCTGTACCAAAAAAAGCTATCAGCCTAATCAATTTGTAACTTTCTTCATAAAATTTGTATGGAAGTTTCTTTCAAGTTTCAACTATGAAGAATTTGTTGTTTATCAATCTATAGCACAATCCTTAAGAAAAAAAATGATCAATTGAATTCTTTTTGCAGTCATCAAGAGAGAGAGCAAGTTTTTGAGCAGCAAACTGTATTGAGACAACTTGAGTCAATATTATCAATTCACAAATTGTCAAGACTGGGGAATCATGTTGATGCATTAAGGGAGGTTGCTAAACTTCCATTTTTGCCTCTAGATCCCCGAGGACCTGATATTGCTGTTGATGTGTTTGAAAATTTATCTCCTCATGTCCAAGCTTGTATACCAGACCTCTTGAAGGTTGCTCTGACTTGTCTGGACAATGTGACAGACTCTGATGGATCACTTCGTGCTTTGAGAGCTAAGGTAATATCCCGTGGATTCCCTTCTTTGGTTGGTCTATATCCATGCTTTAGTTGCTTTTGTTGAAGTACCATAATTTACTAAAACTGTTCTCAACAAGTATTTGGAGCACTGCCAGAAGTAATAAGCTTGTGGCTCTCTTGAATGGAAAGCTTTTTGCTCAATTTGAGAATATAACCTCTATCTTGAAGATGTCATAGGAGTTTGTTATAAAAAGTTTACACAAATATGTGCTCATTATTTTATGTGATAACTACATTTAATACCTACCTTATGCCCTAAATCCAATATATTGTAGTTAGCAGCATTTATTCTGCTGGTCATTGTTTGTTAATATGAAATATACACCATAGATATTAAATCCAAATCTATACTAGGAAAAGGGTGGAAGACAGAAATTTTTTGGTTGA
Protein sequence

>MS.gene36232.t1

MANEDLSSWTDLLHSSSKLLEQAAPSAQFPPLQRNLDQLEALSKKLKSKTVRAEAPSQSIAATRLLAREGINAEQLARDLKSFELKTTFEDVFPVEATSVEEYLQQVHEMAMVSAVQEAQKDNLRSFNDYMMKVLEEDWQKEKRDFLQSLSRISTLPRTNMIANSNVGARPGQIVSMVSTPQVSSGSMVIVPMTSRPIADKKASVYAEVVKNLNRARQSGLPFKLAATFKGAYESLGVDACGGKSVTMRKIWHLVQMLMDEDSTLRRVSKRMSLIIGARRHLEWGHEKYIMDTIHNHPAQASLGGGVGNLQRIRAFLRIRLRDYGVLDFDAGDARRQPPVDTTWQQIYFCLRSGYYDEARNVALSSRASHQFAPLLTEWINTGGMVPEEVATAASEECERMLRTGDRVGRTAYDKKKLLLYAIISGSRRHIDRLLRDQPTLFSTIEDFLWFKLSAVRDCPSGSSSIVLSDGLIPYSLDDLQSYLNKFEPSYYTKNGKDPLVYPYILLLSIQLLPAVLYLSKEAGDEGYNIDAAHLSIVLADHGVLSEGISTGQKLGVMDAYAEVSTIIRQYGSMYLRLGDLQMALEYYAQAAAAVGGGQLSWTGRGNVDQQRQRNLMLKQLLTELLLRDGGIYLLLGARGAGEEGELGRFVADPNARQQFLIEAACQCQEAGMYDKSIEIQKRVGSFSVALDTINKCLSEAICSLFRGRLDGESRTSGLIHSGNEILETYTYYPDVSHQEREQVFEQQTVLRQLESILSIHKLSRLGNHVDALREVAKLPFLPLDPRGPDIAVDVFENLSPHVQACIPDLLKVALTCLDNVTDSDGSLRALRAKEKGGRQKFFG