Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05978.t1 | XP_003630860.2 | 94.9 | 628 | 31 | 1 | 1 | 628 | 124 | 750 | 0.00E+00 | 1183.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05978.t1 | Q2HVD6 | 70.6 | 592 | 82 | 2 | 1 | 592 | 109 | 608 | 3.5e-229 | 795.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05978.t1 | G7L9Q3 | 94.9 | 628 | 31 | 1 | 1 | 628 | 124 | 750 | 0.0e+00 | 1183.3 |
TFs/TRs:
Protein Kinases:
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene05978 | 0.815384 | 9.50E-52 | -1.69E-46 |
| MS.gene049159 | MS.gene05978 | 0.825604 | 4.28E-54 | -1.69E-46 |
| MS.gene049255 | MS.gene05978 | 0.855503 | 6.04E-62 | -1.69E-46 |
| MS.gene049341 | MS.gene05978 | 0.839072 | 1.97E-57 | -1.69E-46 |
| MS.gene049382 | MS.gene05978 | 0.811536 | 6.67E-51 | -1.69E-46 |
| MS.gene049824 | MS.gene05978 | 0.808348 | 3.24E-50 | -1.69E-46 |
| MS.gene049850 | MS.gene05978 | 0.811643 | 6.32E-51 | -1.69E-46 |
| MS.gene050020 | MS.gene05978 | 0.807328 | 5.33E-50 | -1.69E-46 |
| MS.gene050084 | MS.gene05978 | 0.801494 | 8.77E-49 | -1.69E-46 |
| MS.gene050085 | MS.gene05978 | 0.831535 | 1.58E-55 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05978.t1 | MTR_8g104280 | 97.452 | 628 | 15 | 1 | 1 | 628 | 124 | 750 | 0.0 | 1281 |
| MS.gene05978.t1 | MTR_2g099870 | 88.740 | 373 | 42 | 0 | 220 | 592 | 236 | 608 | 0.0 | 694 |
| MS.gene05978.t1 | MTR_2g099870 | 75.352 | 142 | 34 | 1 | 1 | 141 | 109 | 250 | 6.30e-55 | 197 |
| MS.gene05978.t1 | MTR_1g100210 | 36.404 | 228 | 124 | 8 | 356 | 562 | 671 | 898 | 1.48e-34 | 140 |
| MS.gene05978.t1 | MTR_4g025470 | 49.714 | 175 | 33 | 5 | 4 | 177 | 1520 | 1640 | 9.47e-34 | 139 |
| MS.gene05978.t1 | MTR_4g025460 | 59.854 | 137 | 35 | 5 | 487 | 619 | 38 | 158 | 7.55e-31 | 119 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05978.t1 | AT4G10760 | 66.893 | 589 | 154 | 9 | 1 | 586 | 125 | 675 | 0.0 | 747 |
| MS.gene05978.t1 | AT4G09980 | 36.842 | 209 | 97 | 10 | 369 | 549 | 617 | 818 | 1.97e-29 | 125 |
| MS.gene05978.t1 | AT4G09980 | 36.646 | 161 | 69 | 9 | 369 | 503 | 617 | 770 | 4.56e-19 | 92.0 |
Find 0 sgRNAs with CRISPR-Local
Find 265 sgRNAs with CRISPR-GE
CRISPR-Local
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACATATGATGAGGATTTGA+AGG | + | 57363:12217-12236 | MS.gene05978:CDS | 30.0% | |
| AACCTTATTACATGCAACAA+AGG | - | 57363:12592-12611 | MS.gene05978:intergenic | 30.0% | |
| AAGCTTAACAATAACGAATG+TGG | - | 57363:13896-13915 | MS.gene05978:intergenic | 30.0% | |
| AAGTGGCAAATCAAGAATTA+TGG | + | 57363:11803-11822 | MS.gene05978:CDS | 30.0% | |
| AATAAAGTGGAAGAATCGAT+AGG | - | 57363:11627-11646 | MS.gene05978:intergenic | 30.0% | |
| AATATCATACAAACCTCATC+TGG | - | 57363:13708-13727 | MS.gene05978:intergenic | 30.0% | |
| AATCAGCAACAAATTGTTAC+CGG | + | 57363:11933-11952 | MS.gene05978:CDS | 30.0% | |
| AATTCACCACTTACTCTAAT+AGG | + | 57363:12376-12395 | MS.gene05978:intron | 30.0% | |
| AGATGCAAAAATCCAAAACT+GGG | + | 57363:12288-12307 | MS.gene05978:CDS | 30.0% | |
| ATGGACATATTAGGTAAGTT+TGG | + | 57363:13157-13176 | MS.gene05978:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57363 | gene | 11585 | 14709 | 11585 | ID=MS.gene05978 |
| 57363 | mRNA | 11585 | 14709 | 11585 | ID=MS.gene05978.t1;Parent=MS.gene05978 |
| 57363 | exon | 11585 | 12370 | 11585 | ID=MS.gene05978.t1.exon1;Parent=MS.gene05978.t1 |
| 57363 | CDS | 11585 | 12370 | 11585 | ID=cds.MS.gene05978.t1;Parent=MS.gene05978.t1 |
| 57363 | exon | 12510 | 12608 | 12510 | ID=MS.gene05978.t1.exon2;Parent=MS.gene05978.t1 |
| 57363 | CDS | 12510 | 12608 | 12510 | ID=cds.MS.gene05978.t1;Parent=MS.gene05978.t1 |
| 57363 | exon | 12803 | 12886 | 12803 | ID=MS.gene05978.t1.exon3;Parent=MS.gene05978.t1 |
| 57363 | CDS | 12803 | 12886 | 12803 | ID=cds.MS.gene05978.t1;Parent=MS.gene05978.t1 |
| 57363 | exon | 12986 | 13338 | 12986 | ID=MS.gene05978.t1.exon4;Parent=MS.gene05978.t1 |
| 57363 | CDS | 12986 | 13338 | 12986 | ID=cds.MS.gene05978.t1;Parent=MS.gene05978.t1 |
| 57363 | exon | 13497 | 13713 | 13497 | ID=MS.gene05978.t1.exon5;Parent=MS.gene05978.t1 |
| 57363 | CDS | 13497 | 13713 | 13497 | ID=cds.MS.gene05978.t1;Parent=MS.gene05978.t1 |
| 57363 | exon | 14044 | 14123 | 14044 | ID=MS.gene05978.t1.exon6;Parent=MS.gene05978.t1 |
| 57363 | CDS | 14044 | 14123 | 14044 | ID=cds.MS.gene05978.t1;Parent=MS.gene05978.t1 |
| 57363 | exon | 14442 | 14709 | 14442 | ID=MS.gene05978.t1.exon7;Parent=MS.gene05978.t1 |
| 57363 | CDS | 14442 | 14709 | 14442 | ID=cds.MS.gene05978.t1;Parent=MS.gene05978.t1 |
| Gene Sequence |
>MS.gene05978 ATGGTTGCTGTTTGTCTTTTAAGTCGAGTACCTTTTTCACCTATCGATTCTTCCACTTTATTGTGGAAATTAGAGAATGATCAAACGGTTACCCCACAAGACAAAGCCGCACTTCAAGAGCTTGGTGGAGATTCAGGAGGGCCGACACTTGCAGTTGAGATAGCTTTGAGGTCAATGGCAGATGATAATGGTGCTGTTGAATTGGAAGAGTTTGTTGTAAGTGGCAAATCAAGAATTATGGTTTTGAATATAGAGAGGACCCGTCTCTTGAGAGAATTTCCTGAAACTGCACATCATCAGCAGCAGCTGCAAAATGAGTCGAGTCTCGGAGATGGTAATATGAATCAAAATCAGCAACAAATTGTTACCGGTAGTACGAATGTGAACGGTGTTATGGGTGCGATGGGAAGGCCAGTTTTGAGGCCAATATCTGATAATATGTGGATGTCACATGGTGACCCTCACATGCCAGGGTTACAACAAATGTTTTCTGGTGGTGGACCAAGGGGAGCACCAGGACTAATGGGAATGGTGGCTGCACAGAGAGGTATAGTTATTCCGTCGATGCATAGACTCCCAATGGGGCCAAATGCATCAGGGAGTAGTATTAATGCAATGCAACAGAAACCAAAAACATATGATGAGGATTTGAAGGAGGATTTGAAGGAATTTGAGGCCATAATTAATAAGAAATCGTTTAGGGAGATGCAAAAATCCAAAACTGGGAAGGAGCTTTTGGATTTAATTCAGAAACCGACTGCAAGGGAGGCTGCTGTAGCTGCTAAGGTTCAAATTCACCACTTACTCTAATAGGCTATACACTCAAATTTCATTTTTGAATTTTGATTATTCACAGCTTTTACTATACTCTGGCATTTCGAACAGTTTAATCTAATTCTGCTTGTTTAAATGTATTATTTTTCAGTTCAAAACTAAAGGTGGTTCTCAGGTGAAGCAATATTGTGACTTACTGACAAAAGAAGATTGTCGGCGCCAGACCGGTTCCTTTGTTGCATGTAATAAGGTTAATGACAAGAACAATATTTTATTACTAGCTTTTTTTGTTTCTCTGGTTTCATTTCTAACTTATTCACCATCTATTGGTTTTAGCTGGTATACTAAGACATATGATTTTCAACTAATCAAACTATGAAAATTGGTACGGACACTTTTATTTAGCCTTTTGTTGATGTGCATTTGTGATCCTTTTCTCGGCAGGTTCATTTTAGACGAATCATTGCTCTTCACACTGACATTAATTTGGGAGACTGTTCTTTTCTTGATACTTGCCGGCACATGAAGGTATAGTTTTTACCTCGGAGTTCTTATGTTGGATTTTCTCTTCATTTTAGTTATTTTGTCCTATGTTTGAGTTGTGCTCTCTTTTTTGTCCTTTGATAGACATGTAAATATGTTCACTATGAGTATGACCCAACACCTGATTTGCCTCCAACAATGACGGGTGCTCTTCCTCCTCCTCCCAAACCACTAAAACAGCAGCGTGCTGAGTATTGTTCTGAAGTGGAACTTGGTGAACCACAATGGATCAACTGTGATATACGTAACTTTAGAATGGACATATTAGGTAAGTTTGGAGTAATAATGGCTGATCCACCGTGGGACATTCACATGGAGCTGCCTTATGGAACAATGGCGGATGATGAAATGCGCACTCTTAATGTCCCTGCTCTGCAAACTCATGGGCTTATTTTTCTATGGGTCACTGGGCGTGCAATGGAACTTGGACGAGAATGGTATTTGCACCTGTATTTGGTCATATGCTAATTTTTTGTTTTCATTAAAAAAAAATCATCTGATATTCTGATTAGCATAACCAAATAAAAAAAGTTGAGGCTTCTTTTTACTAATAATCTGCATTTGGCTTGCACATATTTCGTATTGTGTTCTGCAGCTTAGAACTTTGGGGATATAAACGTGTTGAGGAGATCATTTGGGTGAAAACAAATCAACTTCAGCGAATAATTAGAACTGGACGTACCGGCCATTGGCTCAATCACAGCAAGGAACATTGTCTCGTTGGGATAAAGGGTAGTCCTGAAGTAAACAGAAACATTGACACCGATGTTATTGTCGCTGAAGTCCGGGAAACAAGCCGAAAGCCAGATGAGGTTTGTATGATATTCAACGAACTTTTTCACTAACAAGTTATTGAAAAGTTGGTTGTTTTTGTTTAGGGTTCCTTAAAAATGTTATTGAAAAGAAGGGTTTACATTATAATAAGCCTGGCTAAAACAAAAACAACTTGCTAACAGCCATGTGCATGATATATTTTCACTTTTCCTCTCCTCCACATTCGTTATTGTTAAGCTTGCATGTTCCATCTTTTAAATAGTTTAACTATGCCTGGTACGGTACTAAAGTTATTCTACCATTTGAGTGTTCAAATTTAAGTCTTTCATATGCGTAACAATTTCAAAATTGTCAATTTATTCCTACAGATGTATCCTTTGTTGGAGAGGATAAGTCCAAGAACAAGAAAGCTGGAGTTGTTTGCTCGCATGCACAATACTCATGCAGGGTTGGTTTTGAATTTTCACGACACTTTTTTTTTGGGAGTTTTTTAATGGTAATCTTAAAATTGTTTGATTGGGGTCGATTATTTTCTGGATTTGAAATGAGTCTTGATGCTACGTAAGTGAAGCTCACTTGATAATGTTCTATATCAATTAAATGATCTCTTGGACTAAAAACCTAATGTGGTTGTAGTTATAGGTTTAGGGCAAAATTTCATCCATAGAAATCATAAACTGTTTTTTATGGTGTTCTTTATTATTTGATATTTGAAGTCATCCAAAGTAATCTTAAGCTGTTATAATATTCTCTTTTGTTCCATCAGGTGGATGTCTCTTGGTAATCAATTGAGTGGTGTAAGGTTAGTAGATGAAGGGCTCCGGGCAAGGTTTAAGGCAGCTTATCCAGATGTGGAGGTGCAGCCAGCATCACCTCCCAGAGCTTCTGCTATGGAGGTAGACTCTAGTGTTGCTGCTCAAACAAGGAGTCCATTTGCCGGCACAGAATCAAAGTCCACTGCTGAGCCTGCAGCTCCAGCAACTACCTCTGCTTCAGAGGAGAAGGCGATGGTCATTGACGTGGACACTAACTAA |
| Protein sequence |
>MS.gene05978.t1 MVAVCLLSRVPFSPIDSSTLLWKLENDQTVTPQDKAALQELGGDSGGPTLAVEIALRSMADDNGAVELEEFVVSGKSRIMVLNIERTRLLREFPETAHHQQQLQNESSLGDGNMNQNQQQIVTGSTNVNGVMGAMGRPVLRPISDNMWMSHGDPHMPGLQQMFSGGGPRGAPGLMGMVAAQRGIVIPSMHRLPMGPNASGSSINAMQQKPKTYDEDLKEDLKEFEAIINKKSFREMQKSKTGKELLDLIQKPTAREAAVAAKFKTKGGSQVKQYCDLLTKEDCRRQTGSFVACNKVHFRRIIALHTDINLGDCSFLDTCRHMKTCKYVHYEYDPTPDLPPTMTGALPPPPKPLKQQRAEYCSEVELGEPQWINCDIRNFRMDILGKFGVIMADPPWDIHMELPYGTMADDEMRTLNVPALQTHGLIFLWVTGRAMELGRECLELWGYKRVEEIIWVKTNQLQRIIRTGRTGHWLNHSKEHCLVGIKGSPEVNRNIDTDVIVAEVRETSRKPDEMYPLLERISPRTRKLELFARMHNTHAGWMSLGNQLSGVRLVDEGLRARFKAAYPDVEVQPASPPRASAMEVDSSVAAQTRSPFAGTESKSTAEPAAPATTSASEEKAMVIDVDTN |
