Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36275.t1 | XP_024631968.1 | 93.3 | 60 | 4 | 0 | 1 | 60 | 50 | 109 | 1.20E-24 | 121.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36275.t1 | Q38832 | 66.7 | 54 | 17 | 1 | 2 | 55 | 160 | 212 | 2.7e-14 | 78.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36275.t1 | G7IMD8 | 93.3 | 60 | 4 | 0 | 1 | 60 | 130 | 189 | 8.3e-25 | 121.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05288 | MS.gene36275 | 0.814618 | 1.41E-51 | -1.69E-46 |
| MS.gene05289 | MS.gene36275 | 0.807297 | 5.41E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAATAGGAATGAATACA+TGG | - | 15225:17839-17858 | MS.gene36275:CDS | 20.0% |
| !! | TTTGATGAATATATACTCTA+AGG | + | 15225:18084-18103 | None:intergenic | 20.0% |
| ! | AAATAATAACTAGCTGTAGA+TGG | + | 15225:18017-18036 | None:intergenic | 25.0% |
| ! | ATATATATGAACTTACTTCC+AGG | + | 15225:17913-17932 | None:intergenic | 25.0% |
| ! | TATATATGAACTTACTTCCA+GGG | + | 15225:17912-17931 | None:intergenic | 25.0% |
| !! | TCATGGATTCTAGAAAAAAT+AGG | - | 15225:17826-17845 | MS.gene36275:CDS | 25.0% |
| !!! | TTTTTTTCTTTCTTTCCTCT+AGG | - | 15225:18159-18178 | MS.gene36275:CDS | 25.0% |
| GATGAATGAGAAGAAATTCA+TGG | - | 15225:17809-17828 | MS.gene36275:CDS | 30.0% | |
| TTGAATCATGTAAGAGGATA+AGG | - | 15225:18189-18208 | MS.gene36275:CDS | 30.0% | |
| ! | TGTTTGTTGAATCATGTAAG+AGG | - | 15225:18183-18202 | MS.gene36275:CDS | 30.0% |
| GCTACATACGAAGATAAAGA+TGG | - | 15225:17861-17880 | MS.gene36275:intron | 35.0% | |
| TGATTCAACAAACATCCTAG+AGG | + | 15225:18177-18196 | None:intergenic | 35.0% | |
| ! | GATAAGGCTTATGATTAGCT+CGG | - | 15225:18205-18224 | MS.gene36275:CDS | 35.0% |
| !!! | TCGGAGGACAATATTGATTT+TGG | - | 15225:18224-18243 | MS.gene36275:CDS | 35.0% |
| ACGAAGATAAAGATGGTGAC+TGG | - | 15225:17868-17887 | MS.gene36275:intron | 40.0% | |
| ! | AAGGCTTATGATTAGCTCGG+AGG | - | 15225:18208-18227 | MS.gene36275:CDS | 45.0% |
| ! | ATGGTGACTGGATGTTGCTT+GGG | - | 15225:17880-17899 | MS.gene36275:intron | 45.0% |
| GATGGTGACTGGATGTTGCT+TGG | - | 15225:17879-17898 | MS.gene36275:intron | 50.0% | |
| ! | TGGTGACTGGATGTTGCTTG+GGG | - | 15225:17881-17900 | MS.gene36275:intron | 50.0% |
| ! | TGTTGCTTGGGGATGCACCC+TGG | - | 15225:17892-17911 | MS.gene36275:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 15225 | gene | 17799 | 18245 | 17799 | ID=MS.gene36275 |
| 15225 | mRNA | 17799 | 18245 | 17799 | ID=MS.gene36275.t1;Parent=MS.gene36275 |
| 15225 | exon | 18128 | 18245 | 18128 | ID=MS.gene36275.t1.exon1;Parent=MS.gene36275.t1 |
| 15225 | CDS | 18128 | 18245 | 18128 | ID=cds.MS.gene36275.t1;Parent=MS.gene36275.t1 |
| 15225 | exon | 17799 | 17863 | 17799 | ID=MS.gene36275.t1.exon2;Parent=MS.gene36275.t1 |
| 15225 | CDS | 17799 | 17863 | 17799 | ID=cds.MS.gene36275.t1;Parent=MS.gene36275.t1 |
| Gene Sequence |
| Protein sequence |
