Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05289.t1 | KEH39385.1 | 63 | 81 | 19 | 1 | 19 | 88 | 375 | 455 | 1.00E-13 | 85.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05289.t1 | Q9LHB1 | 50.0 | 58 | 29 | 0 | 22 | 79 | 380 | 437 | 2.3e-06 | 52.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05289.t1 | A0A072VMP6 | 63.0 | 81 | 19 | 1 | 19 | 88 | 375 | 455 | 7.4e-14 | 85.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene05289 | 0.821665 | 3.58E-53 | -1.69E-46 |
| MS.gene050605 | MS.gene05289 | 0.812224 | 4.72E-51 | -1.69E-46 |
| MS.gene050680 | MS.gene05289 | 0.800264 | 1.56E-48 | -1.69E-46 |
| MS.gene052426 | MS.gene05289 | 0.804501 | 2.10E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene05289 | 0.934884 | 2.02E-96 | -1.69E-46 |
| MS.gene05289 | MS.gene053008 | 0.816586 | 5.12E-52 | -1.69E-46 |
| MS.gene05289 | MS.gene053166 | 0.804767 | 1.84E-49 | -1.69E-46 |
| MS.gene05289 | MS.gene053584 | 0.834321 | 3.21E-56 | -1.69E-46 |
| MS.gene05289 | MS.gene054236 | 0.813375 | 2.64E-51 | -1.69E-46 |
| MS.gene05289 | MS.gene054559 | 0.802743 | 4.85E-49 | -1.69E-46 |
| MS.gene05289 | MS.gene054713 | 0.805691 | 1.18E-49 | -1.69E-46 |
| MS.gene05289 | MS.gene05510 | 0.813589 | 2.37E-51 | -1.69E-46 |
| MS.gene05289 | MS.gene059128 | 0.805898 | 1.07E-49 | -1.69E-46 |
| MS.gene05289 | MS.gene060011 | 0.825586 | 4.32E-54 | -1.69E-46 |
| MS.gene05289 | MS.gene060322 | 0.816531 | 5.27E-52 | -1.69E-46 |
| MS.gene05289 | MS.gene060802 | 0.805156 | 1.53E-49 | -1.69E-46 |
| MS.gene05289 | MS.gene060803 | 0.800224 | 1.59E-48 | -1.69E-46 |
| MS.gene05289 | MS.gene060998 | 0.810241 | 1.27E-50 | -1.69E-46 |
| MS.gene05289 | MS.gene062926 | 0.802604 | 5.19E-49 | -1.69E-46 |
| MS.gene05289 | MS.gene06303 | 0.800675 | 1.29E-48 | -1.69E-46 |
| MS.gene05289 | MS.gene064070 | 0.827126 | 1.86E-54 | -1.69E-46 |
| MS.gene05289 | MS.gene065054 | 0.812458 | 4.20E-51 | -1.69E-46 |
| MS.gene05289 | MS.gene065863 | 0.834847 | 2.37E-56 | -1.69E-46 |
| MS.gene05289 | MS.gene065865 | 0.835674 | 1.46E-56 | -1.69E-46 |
| MS.gene05289 | MS.gene065867 | 0.846965 | 1.56E-59 | -1.69E-46 |
| MS.gene05289 | MS.gene066206 | 0.808879 | 2.49E-50 | -1.69E-46 |
| MS.gene05289 | MS.gene066320 | 0.802819 | 4.68E-49 | -1.69E-46 |
| MS.gene05289 | MS.gene06697 | 0.807819 | 4.19E-50 | -1.69E-46 |
| MS.gene05289 | MS.gene067163 | 0.823173 | 1.60E-53 | -1.69E-46 |
| MS.gene05289 | MS.gene07344 | 0.802963 | 4.37E-49 | -1.69E-46 |
| MS.gene05289 | MS.gene073460 | 0.812798 | 3.54E-51 | -1.69E-46 |
| MS.gene05289 | MS.gene20635 | 0.804767 | 1.84E-49 | -1.69E-46 |
| MS.gene05289 | MS.gene21911 | 0.810874 | 9.28E-51 | -1.69E-46 |
| MS.gene05289 | MS.gene22091 | 0.878581 | 2.50E-69 | -1.69E-46 |
| MS.gene05289 | MS.gene22731 | 0.801285 | 9.68E-49 | -1.69E-46 |
| MS.gene05289 | MS.gene22833 | 0.817694 | 2.89E-52 | -1.69E-46 |
| MS.gene05289 | MS.gene23945 | 0.806385 | 8.44E-50 | -1.69E-46 |
| MS.gene05289 | MS.gene28659 | 0.836719 | 7.93E-57 | -1.69E-46 |
| MS.gene05289 | MS.gene31283 | 0.805394 | 1.36E-49 | -1.69E-46 |
| MS.gene05289 | MS.gene31397 | 0.809442 | 1.89E-50 | -1.69E-46 |
| MS.gene05289 | MS.gene31520 | 0.813764 | 2.17E-51 | -1.69E-46 |
| MS.gene05289 | MS.gene35408 | 0.814982 | 1.17E-51 | -1.69E-46 |
| MS.gene05289 | MS.gene35823 | 0.80161 | 8.30E-49 | -1.69E-46 |
| MS.gene05289 | MS.gene35824 | 0.800337 | 1.51E-48 | -1.69E-46 |
| MS.gene05289 | MS.gene35864 | 0.800017 | 1.76E-48 | -1.69E-46 |
| MS.gene05289 | MS.gene36275 | 0.807297 | 5.41E-50 | -1.69E-46 |
| MS.gene05289 | MS.gene42125 | 0.823828 | 1.12E-53 | -1.69E-46 |
| MS.gene05289 | MS.gene43040 | 0.813702 | 2.24E-51 | -1.69E-46 |
| MS.gene05289 | MS.gene46374 | 0.826483 | 2.65E-54 | -1.69E-46 |
| MS.gene05289 | MS.gene49839 | 0.808479 | 3.04E-50 | -1.69E-46 |
| MS.gene05289 | MS.gene49940 | 0.82846 | 8.88E-55 | -1.69E-46 |
| MS.gene05289 | MS.gene50708 | 0.810801 | 9.62E-51 | -1.69E-46 |
| MS.gene05289 | MS.gene51459 | 0.81553 | 8.82E-52 | -1.69E-46 |
| MS.gene05289 | MS.gene54876 | 0.813305 | 2.74E-51 | -1.69E-46 |
| MS.gene05289 | MS.gene55539 | 0.801204 | 1.01E-48 | -1.69E-46 |
| MS.gene05289 | MS.gene55625 | 0.814837 | 1.26E-51 | -1.69E-46 |
| MS.gene05289 | MS.gene56053 | 0.806772 | 6.99E-50 | -1.69E-46 |
| MS.gene05289 | MS.gene56057 | 0.829945 | 3.87E-55 | -1.69E-46 |
| MS.gene05289 | MS.gene56431 | 0.818258 | 2.15E-52 | -1.69E-46 |
| MS.gene05289 | MS.gene56840 | 0.833908 | 4.07E-56 | -1.69E-46 |
| MS.gene05289 | MS.gene57469 | 0.812608 | 3.89E-51 | -1.69E-46 |
| MS.gene05289 | MS.gene58503 | 0.805746 | 1.15E-49 | -1.69E-46 |
| MS.gene05289 | MS.gene58865 | 0.912414 | 2.00E-83 | -1.69E-46 |
| MS.gene05289 | MS.gene58910 | 0.903568 | 3.03E-79 | -1.69E-46 |
| MS.gene05289 | MS.gene59355 | 0.817452 | 3.27E-52 | -1.69E-46 |
| MS.gene05289 | MS.gene59948 | 0.812317 | 4.51E-51 | -1.69E-46 |
| MS.gene05289 | MS.gene60903 | 0.889941 | 1.54E-73 | -1.69E-46 |
| MS.gene05289 | MS.gene61207 | 0.810566 | 1.08E-50 | -1.69E-46 |
| MS.gene05289 | MS.gene61435 | 0.805001 | 1.65E-49 | -1.69E-46 |
| MS.gene05289 | MS.gene61522 | 0.816771 | 4.66E-52 | -1.69E-46 |
| MS.gene05289 | MS.gene64417 | 0.828266 | 9.89E-55 | -1.69E-46 |
| MS.gene05289 | MS.gene65895 | 0.816498 | 5.36E-52 | -1.69E-46 |
| MS.gene05289 | MS.gene69794 | 0.811079 | 8.37E-51 | -1.69E-46 |
| MS.gene05289 | MS.gene69867 | 0.810623 | 1.05E-50 | -1.69E-46 |
| MS.gene05289 | MS.gene70534 | 0.800733 | 1.26E-48 | -1.69E-46 |
| MS.gene05289 | MS.gene71203 | 0.820981 | 5.15E-53 | -1.69E-46 |
| MS.gene05289 | MS.gene72268 | 0.813881 | 2.05E-51 | -1.69E-46 |
| MS.gene05289 | MS.gene73257 | 0.830856 | 2.32E-55 | -1.69E-46 |
| MS.gene05289 | MS.gene74971 | 0.822121 | 2.81E-53 | -1.69E-46 |
| MS.gene05289 | MS.gene76226 | 0.811812 | 5.81E-51 | -1.69E-46 |
| MS.gene05289 | MS.gene77188 | 0.817855 | 2.66E-52 | -1.69E-46 |
| MS.gene05289 | MS.gene78492 | 0.800554 | 1.36E-48 | -1.69E-46 |
| MS.gene05289 | MS.gene82879 | 0.84167 | 4.13E-58 | -1.69E-46 |
| MS.gene05289 | MS.gene84326 | 0.817811 | 2.72E-52 | -1.69E-46 |
| MS.gene05289 | MS.gene87354 | 0.800662 | 1.30E-48 | -1.69E-46 |
| MS.gene05289 | MS.gene87897 | 0.807178 | 5.74E-50 | -1.69E-46 |
| MS.gene05289 | MS.gene89109 | 0.834884 | 2.32E-56 | -1.69E-46 |
| MS.gene05289 | MS.gene90525 | 0.825683 | 4.10E-54 | -1.69E-46 |
| MS.gene05289 | MS.gene92590 | 0.814088 | 1.84E-51 | -1.69E-46 |
| MS.gene05289 | MS.gene93638 | 0.813614 | 2.34E-51 | -1.69E-46 |
| MS.gene05289 | MS.gene98325 | 0.806684 | 7.30E-50 | -1.69E-46 |
| MS.gene05289 | MS.gene98774 | 0.821506 | 3.89E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05289.t1 | MTR_4g126200 | 66.667 | 51 | 17 | 0 | 38 | 88 | 3 | 53 | 1.45e-13 | 63.9 |
| MS.gene05289.t1 | MTR_2g095400 | 68.750 | 64 | 20 | 0 | 19 | 82 | 375 | 438 | 2.06e-12 | 61.2 |
| MS.gene05289.t1 | MTR_5g079940 | 50.000 | 62 | 31 | 0 | 19 | 80 | 859 | 920 | 3.52e-12 | 60.8 |
| MS.gene05289.t1 | MTR_2g097010 | 67.188 | 64 | 21 | 0 | 19 | 82 | 401 | 464 | 2.37e-11 | 58.2 |
| MS.gene05289.t1 | MTR_2g097020 | 60.870 | 46 | 18 | 0 | 38 | 83 | 409 | 454 | 7.00e-11 | 57.0 |
| MS.gene05289.t1 | MTR_3g067815 | 50.820 | 61 | 30 | 0 | 22 | 82 | 382 | 442 | 7.33e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05289.t1 | AT4G01180 | 49.180 | 61 | 31 | 0 | 16 | 76 | 277 | 337 | 5.36e-11 | 57.4 |
Find 13 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTGATGCAAGACATGCTT+TGG | 0.365400 | 6.3:+74073129 | MS.gene05289:CDS |
| TGCAAGACATGCTTTGGAAT+TGG | 0.380086 | 6.3:+74073135 | MS.gene05289:CDS |
| AAAGAAGAAATGGCGGAAGC+TGG | 0.447708 | 6.3:+74072867 | None:intergenic |
| ATTGCATCACTTATCTCGTC+TGG | 0.458810 | 6.3:-74072912 | None:intergenic |
| TTGGATGTTGCAGAAAGAAA+AGG | 0.471032 | 6.3:+74073069 | MS.gene05289:intron |
| AAGAAGAAATGGCGGAAGCT+GGG | 0.487627 | 6.3:+74072868 | None:intergenic |
| TGCAATATCAGATCAACAAA+AGG | 0.494458 | 6.3:+74072929 | MS.gene05289:CDS |
| GCGTTTGGCTGAAGACCATA+AGG | 0.535802 | 6.3:+74072968 | MS.gene05289:CDS |
| ATATCAGATCAACAAAAGGC+TGG | 0.578847 | 6.3:+74072933 | MS.gene05289:CDS |
| AGAAGAAATGGCGGAAGCTG+GGG | 0.585508 | 6.3:+74072869 | None:intergenic |
| AGAAATGGCGGAAGCTGGGG+TGG | 0.586264 | 6.3:+74072872 | None:intergenic |
| TCACAAGAAAATGCATGACC+TGG | 0.631525 | 6.3:+74073099 | MS.gene05289:CDS |
| TTGGAGATAGAACAGCTGAA+AGG | 0.635079 | 6.3:+74073154 | MS.gene05289:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTAAAAAATGTTGATAAA+AGG | - | chr6.3:74073009-74073028 | None:intergenic | 10.0% |
| ! | AAAATCAAATGTGTACCTTA+TGG | - | chr6.3:74072986-74073005 | None:intergenic | 25.0% |
| !!! | TTATGTTTTGATGACTCTTT+TGG | + | chr6.3:74073046-74073065 | MS.gene05289:intron | 25.0% |
| AAGAGTCATCAAAACATAAG+TGG | - | chr6.3:74073045-74073064 | None:intergenic | 30.0% | |
| TGCAATATCAGATCAACAAA+AGG | + | chr6.3:74072929-74072948 | MS.gene05289:CDS | 30.0% | |
| TTTCCGTGTAATGAAAAAAC+TGG | + | chr6.3:74073180-74073199 | MS.gene05289:CDS | 30.0% | |
| ATATCAGATCAACAAAAGGC+TGG | + | chr6.3:74072933-74072952 | MS.gene05289:CDS | 35.0% | |
| TGGTGAAAAAATGATGCGTT+TGG | + | chr6.3:74072953-74072972 | MS.gene05289:CDS | 35.0% | |
| TTGGATGTTGCAGAAAGAAA+AGG | + | chr6.3:74073069-74073088 | MS.gene05289:intron | 35.0% | |
| !! | GTTTTGATGACTCTTTTGGT+TGG | + | chr6.3:74073050-74073069 | MS.gene05289:intron | 35.0% |
| !!! | CCTCCAGTTTTTTCATTACA+CGG | - | chr6.3:74073186-74073205 | None:intergenic | 35.0% |
| ACTTGATGCAAGACATGCTT+TGG | + | chr6.3:74073129-74073148 | MS.gene05289:CDS | 40.0% | |
| ATTGCATCACTTATCTCGTC+TGG | - | chr6.3:74072915-74072934 | None:intergenic | 40.0% | |
| CCGTGTAATGAAAAAACTGG+AGG | + | chr6.3:74073183-74073202 | MS.gene05289:CDS | 40.0% | |
| TCACAAGAAAATGCATGACC+TGG | + | chr6.3:74073099-74073118 | MS.gene05289:CDS | 40.0% | |
| TTGGAGATAGAACAGCTGAA+AGG | + | chr6.3:74073154-74073173 | MS.gene05289:CDS | 40.0% | |
| ! | TTGCATCAAGTCTTTTCACC+AGG | - | chr6.3:74073120-74073139 | None:intergenic | 40.0% |
| !! | TGCAAGACATGCTTTGGAAT+TGG | + | chr6.3:74073135-74073154 | MS.gene05289:CDS | 40.0% |
| GCGTTTGGCTGAAGACCATA+AGG | + | chr6.3:74072968-74072987 | MS.gene05289:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 74072876 | 74073234 | 74072876 | ID=MS.gene05289 |
| chr6.3 | mRNA | 74072876 | 74073234 | 74072876 | ID=MS.gene05289.t1;Parent=MS.gene05289 |
| chr6.3 | exon | 74072876 | 74072989 | 74072876 | ID=MS.gene05289.t1.exon1;Parent=MS.gene05289.t1 |
| chr6.3 | CDS | 74072876 | 74072989 | 74072876 | ID=cds.MS.gene05289.t1;Parent=MS.gene05289.t1 |
| chr6.3 | exon | 74073082 | 74073234 | 74073082 | ID=MS.gene05289.t1.exon2;Parent=MS.gene05289.t1 |
| chr6.3 | CDS | 74073082 | 74073234 | 74073082 | ID=cds.MS.gene05289.t1;Parent=MS.gene05289.t1 |
| Gene Sequence |
| Protein sequence |
