Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05288.t1 | KEH39385.1 | 63 | 81 | 19 | 1 | 19 | 88 | 375 | 455 | 1.00E-13 | 85.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05288.t1 | Q9LHB1 | 50.0 | 58 | 29 | 0 | 22 | 79 | 380 | 437 | 2.3e-06 | 52.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05288.t1 | A0A072VMP6 | 63.0 | 81 | 19 | 1 | 19 | 88 | 375 | 455 | 7.4e-14 | 85.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene05288 | 0.834108 | 3.63E-56 | -1.69E-46 |
| MS.gene049501 | MS.gene05288 | 0.800932 | 1.14E-48 | -1.69E-46 |
| MS.gene050442 | MS.gene05288 | 0.807612 | 4.64E-50 | -1.69E-46 |
| MS.gene050605 | MS.gene05288 | 0.811802 | 5.83E-51 | -1.69E-46 |
| MS.gene050844 | MS.gene05288 | 0.805442 | 1.33E-49 | -1.69E-46 |
| MS.gene052863 | MS.gene05288 | 0.805387 | 1.37E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene05289 | 0.934884 | 2.02E-96 | -1.69E-46 |
| MS.gene05288 | MS.gene053008 | 0.830429 | 2.95E-55 | -1.69E-46 |
| MS.gene05288 | MS.gene053584 | 0.840255 | 9.71E-58 | -1.69E-46 |
| MS.gene05288 | MS.gene054236 | 0.802777 | 4.77E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene054713 | 0.817646 | 2.96E-52 | -1.69E-46 |
| MS.gene05288 | MS.gene05510 | 0.819906 | 9.08E-53 | -1.69E-46 |
| MS.gene05288 | MS.gene055818 | 0.801475 | 8.85E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene058146 | 0.80672 | 7.17E-50 | -1.69E-46 |
| MS.gene05288 | MS.gene058595 | 0.803593 | 3.24E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene059842 | 0.810638 | 1.04E-50 | -1.69E-46 |
| MS.gene05288 | MS.gene060011 | 0.816454 | 5.49E-52 | -1.69E-46 |
| MS.gene05288 | MS.gene060322 | 0.831983 | 1.23E-55 | -1.69E-46 |
| MS.gene05288 | MS.gene060685 | 0.800458 | 1.43E-48 | -1.69E-46 |
| MS.gene05288 | MS.gene060802 | 0.819638 | 1.05E-52 | -1.69E-46 |
| MS.gene05288 | MS.gene060803 | 0.819879 | 9.21E-53 | -1.69E-46 |
| MS.gene05288 | MS.gene060998 | 0.805938 | 1.05E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene062926 | 0.821282 | 4.39E-53 | -1.69E-46 |
| MS.gene05288 | MS.gene06303 | 0.804035 | 2.62E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene064070 | 0.838369 | 3.00E-57 | -1.69E-46 |
| MS.gene05288 | MS.gene064460 | 0.808052 | 3.74E-50 | -1.69E-46 |
| MS.gene05288 | MS.gene065054 | 0.825914 | 3.61E-54 | -1.69E-46 |
| MS.gene05288 | MS.gene065863 | 0.845757 | 3.33E-59 | -1.69E-46 |
| MS.gene05288 | MS.gene065865 | 0.826402 | 2.77E-54 | -1.69E-46 |
| MS.gene05288 | MS.gene065867 | 0.844919 | 5.61E-59 | -1.69E-46 |
| MS.gene05288 | MS.gene066206 | 0.822308 | 2.54E-53 | -1.69E-46 |
| MS.gene05288 | MS.gene066320 | 0.803039 | 4.22E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene06697 | 0.814296 | 1.66E-51 | -1.69E-46 |
| MS.gene05288 | MS.gene067163 | 0.814702 | 1.35E-51 | -1.69E-46 |
| MS.gene05288 | MS.gene067566 | 0.80749 | 4.93E-50 | -1.69E-46 |
| MS.gene05288 | MS.gene068245 | 0.813462 | 2.53E-51 | -1.69E-46 |
| MS.gene05288 | MS.gene068838 | 0.813877 | 2.05E-51 | -1.69E-46 |
| MS.gene05288 | MS.gene069088 | 0.828113 | 1.08E-54 | -1.69E-46 |
| MS.gene05288 | MS.gene072023 | 0.81512 | 1.09E-51 | -1.69E-46 |
| MS.gene05288 | MS.gene07344 | 0.814451 | 1.53E-51 | -1.69E-46 |
| MS.gene05288 | MS.gene073460 | 0.830792 | 2.41E-55 | -1.69E-46 |
| MS.gene05288 | MS.gene074026 | 0.803921 | 2.77E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene07532 | 0.813399 | 2.61E-51 | -1.69E-46 |
| MS.gene05288 | MS.gene20635 | 0.811449 | 6.96E-51 | -1.69E-46 |
| MS.gene05288 | MS.gene21911 | 0.819715 | 1.00E-52 | -1.69E-46 |
| MS.gene05288 | MS.gene22091 | 0.89563 | 7.98E-76 | -1.69E-46 |
| MS.gene05288 | MS.gene22833 | 0.819629 | 1.05E-52 | -1.69E-46 |
| MS.gene05288 | MS.gene23945 | 0.808933 | 2.43E-50 | -1.69E-46 |
| MS.gene05288 | MS.gene24642 | 0.808254 | 3.39E-50 | -1.69E-46 |
| MS.gene05288 | MS.gene24651 | 0.807978 | 3.88E-50 | -1.69E-46 |
| MS.gene05288 | MS.gene27008 | 0.807209 | 5.65E-50 | -1.69E-46 |
| MS.gene05288 | MS.gene27271 | 0.803229 | 3.85E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene28658 | 0.801964 | 7.02E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene28659 | 0.820207 | 7.75E-53 | -1.69E-46 |
| MS.gene05288 | MS.gene28842 | 0.80687 | 6.67E-50 | -1.69E-46 |
| MS.gene05288 | MS.gene29000 | 0.813671 | 2.27E-51 | -1.69E-46 |
| MS.gene05288 | MS.gene31283 | 0.801586 | 8.40E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene31520 | 0.84479 | 6.08E-59 | -1.69E-46 |
| MS.gene05288 | MS.gene35408 | 0.828708 | 7.74E-55 | -1.69E-46 |
| MS.gene05288 | MS.gene35822 | 0.815011 | 1.15E-51 | -1.69E-46 |
| MS.gene05288 | MS.gene35823 | 0.820011 | 8.59E-53 | -1.69E-46 |
| MS.gene05288 | MS.gene35824 | 0.820183 | 7.85E-53 | -1.69E-46 |
| MS.gene05288 | MS.gene35864 | 0.817906 | 2.59E-52 | -1.69E-46 |
| MS.gene05288 | MS.gene35867 | 0.816965 | 4.21E-52 | -1.69E-46 |
| MS.gene05288 | MS.gene36275 | 0.814618 | 1.41E-51 | -1.69E-46 |
| MS.gene05288 | MS.gene42125 | 0.818694 | 1.72E-52 | -1.69E-46 |
| MS.gene05288 | MS.gene43040 | 0.822827 | 1.92E-53 | -1.69E-46 |
| MS.gene05288 | MS.gene44451 | 0.802436 | 5.62E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene44502 | 0.803846 | 2.87E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene46374 | 0.838583 | 2.64E-57 | -1.69E-46 |
| MS.gene05288 | MS.gene47283 | 0.82315 | 1.62E-53 | -1.69E-46 |
| MS.gene05288 | MS.gene48988 | 0.801653 | 8.13E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene49839 | 0.833771 | 4.40E-56 | -1.69E-46 |
| MS.gene05288 | MS.gene49940 | 0.826604 | 2.48E-54 | -1.69E-46 |
| MS.gene05288 | MS.gene50708 | 0.817203 | 3.72E-52 | -1.69E-46 |
| MS.gene05288 | MS.gene51459 | 0.807959 | 3.92E-50 | -1.69E-46 |
| MS.gene05288 | MS.gene55539 | 0.815066 | 1.12E-51 | -1.69E-46 |
| MS.gene05288 | MS.gene55625 | 0.81999 | 8.69E-53 | -1.69E-46 |
| MS.gene05288 | MS.gene56057 | 0.814549 | 1.46E-51 | -1.69E-46 |
| MS.gene05288 | MS.gene56431 | 0.819989 | 8.69E-53 | -1.69E-46 |
| MS.gene05288 | MS.gene56840 | 0.824743 | 6.84E-54 | -1.69E-46 |
| MS.gene05288 | MS.gene57469 | 0.831007 | 2.13E-55 | -1.69E-46 |
| MS.gene05288 | MS.gene58503 | 0.80398 | 2.69E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene58865 | 0.92507 | 3.02E-90 | -1.69E-46 |
| MS.gene05288 | MS.gene58910 | 0.92514 | 2.75E-90 | -1.69E-46 |
| MS.gene05288 | MS.gene59355 | 0.83811 | 3.49E-57 | -1.69E-46 |
| MS.gene05288 | MS.gene59442 | 0.821057 | 4.94E-53 | -1.69E-46 |
| MS.gene05288 | MS.gene59948 | 0.826194 | 3.10E-54 | -1.69E-46 |
| MS.gene05288 | MS.gene59949 | 0.818332 | 2.07E-52 | -1.69E-46 |
| MS.gene05288 | MS.gene60903 | 0.886346 | 3.70E-72 | -1.69E-46 |
| MS.gene05288 | MS.gene61207 | 0.831654 | 1.48E-55 | -1.69E-46 |
| MS.gene05288 | MS.gene61210 | 0.807343 | 5.29E-50 | -1.69E-46 |
| MS.gene05288 | MS.gene61435 | 0.805642 | 1.21E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene61522 | 0.841227 | 5.40E-58 | -1.69E-46 |
| MS.gene05288 | MS.gene62393 | 0.817154 | 3.82E-52 | -1.69E-46 |
| MS.gene05288 | MS.gene64417 | 0.835917 | 1.27E-56 | -1.69E-46 |
| MS.gene05288 | MS.gene64740 | 0.804378 | 2.22E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene65221 | 0.80254 | 5.34E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene65718 | 0.802984 | 4.33E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene65895 | 0.824942 | 6.14E-54 | -1.69E-46 |
| MS.gene05288 | MS.gene68036 | 0.823357 | 1.45E-53 | -1.69E-46 |
| MS.gene05288 | MS.gene68582 | 0.821746 | 3.43E-53 | -1.69E-46 |
| MS.gene05288 | MS.gene68987 | 0.821473 | 3.96E-53 | -1.69E-46 |
| MS.gene05288 | MS.gene69575 | 0.805227 | 1.48E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene69794 | 0.819245 | 1.29E-52 | -1.69E-46 |
| MS.gene05288 | MS.gene70895 | 0.802604 | 5.19E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene71203 | 0.832103 | 1.14E-55 | -1.69E-46 |
| MS.gene05288 | MS.gene71871 | 0.805943 | 1.05E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene72253 | 0.805649 | 1.21E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene72268 | 0.814625 | 1.40E-51 | -1.69E-46 |
| MS.gene05288 | MS.gene73257 | 0.834885 | 2.31E-56 | -1.69E-46 |
| MS.gene05288 | MS.gene73522 | 0.804399 | 2.20E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene73528 | 0.807406 | 5.14E-50 | -1.69E-46 |
| MS.gene05288 | MS.gene74971 | 0.841549 | 4.44E-58 | -1.69E-46 |
| MS.gene05288 | MS.gene76226 | 0.825011 | 5.91E-54 | -1.69E-46 |
| MS.gene05288 | MS.gene76373 | 0.809351 | 1.97E-50 | -1.69E-46 |
| MS.gene05288 | MS.gene77188 | 0.814386 | 1.58E-51 | -1.69E-46 |
| MS.gene05288 | MS.gene78234 | 0.800189 | 1.62E-48 | -1.69E-46 |
| MS.gene05288 | MS.gene78492 | 0.802994 | 4.31E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene82879 | 0.832953 | 7.04E-56 | -1.69E-46 |
| MS.gene05288 | MS.gene84326 | 0.832058 | 1.17E-55 | -1.69E-46 |
| MS.gene05288 | MS.gene84948 | 0.803707 | 3.07E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene86844 | 0.80554 | 1.27E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene87430 | 0.81669 | 4.86E-52 | -1.69E-46 |
| MS.gene05288 | MS.gene87897 | 0.822658 | 2.11E-53 | -1.69E-46 |
| MS.gene05288 | MS.gene88788 | 0.819474 | 1.14E-52 | -1.69E-46 |
| MS.gene05288 | MS.gene89109 | 0.845043 | 5.19E-59 | -1.69E-46 |
| MS.gene05288 | MS.gene90525 | 0.827016 | 1.97E-54 | -1.69E-46 |
| MS.gene05288 | MS.gene91907 | 0.804789 | 1.82E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene92590 | 0.851554 | 8.23E-61 | -1.69E-46 |
| MS.gene05288 | MS.gene98071 | 0.801104 | 1.05E-48 | -1.69E-46 |
| MS.gene05288 | MS.gene98543 | 0.801387 | 9.22E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05288.t1 | MTR_4g126200 | 66.667 | 51 | 17 | 0 | 38 | 88 | 3 | 53 | 1.45e-13 | 63.9 |
| MS.gene05288.t1 | MTR_2g095400 | 68.750 | 64 | 20 | 0 | 19 | 82 | 375 | 438 | 2.06e-12 | 61.2 |
| MS.gene05288.t1 | MTR_5g079940 | 50.000 | 62 | 31 | 0 | 19 | 80 | 859 | 920 | 3.52e-12 | 60.8 |
| MS.gene05288.t1 | MTR_2g097010 | 67.188 | 64 | 21 | 0 | 19 | 82 | 401 | 464 | 2.37e-11 | 58.2 |
| MS.gene05288.t1 | MTR_2g097020 | 60.870 | 46 | 18 | 0 | 38 | 83 | 409 | 454 | 7.00e-11 | 57.0 |
| MS.gene05288.t1 | MTR_3g067815 | 50.820 | 61 | 30 | 0 | 22 | 82 | 382 | 442 | 7.33e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05288.t1 | AT4G01180 | 49.180 | 61 | 31 | 0 | 16 | 76 | 277 | 337 | 5.36e-11 | 57.4 |
Find 13 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTGATGCAAGACATGCTT+TGG | 0.365400 | 6.3:-74033146 | MS.gene05288:CDS |
| TGCAAGACATGCTTTGGAAT+TGG | 0.380086 | 6.3:-74033140 | MS.gene05288:CDS |
| AAAGAAGAAATGGCGGAAGC+TGG | 0.447708 | 6.3:-74033408 | None:intergenic |
| ATTGCATCACTTATCTCGTC+TGG | 0.458810 | 6.3:+74033363 | None:intergenic |
| TTGGATGTTGCAGAAAGAAA+AGG | 0.471032 | 6.3:-74033206 | MS.gene05288:intron |
| AAGAAGAAATGGCGGAAGCT+GGG | 0.487627 | 6.3:-74033407 | None:intergenic |
| TGCAATATCAGATCAACAAA+AGG | 0.494458 | 6.3:-74033346 | MS.gene05288:CDS |
| GCGTTTGGCTGAAGACCATA+AGG | 0.535802 | 6.3:-74033307 | MS.gene05288:intron |
| ATATCAGATCAACAAAAGGC+TGG | 0.578847 | 6.3:-74033342 | MS.gene05288:CDS |
| AGAAGAAATGGCGGAAGCTG+GGG | 0.585508 | 6.3:-74033406 | None:intergenic |
| AGAAATGGCGGAAGCTGGGG+TGG | 0.586264 | 6.3:-74033403 | None:intergenic |
| TCACAAGAAAATGCATGACC+TGG | 0.631525 | 6.3:-74033176 | MS.gene05288:CDS |
| TTGGAGATAGAACAGCTGAA+AGG | 0.635079 | 6.3:-74033121 | MS.gene05288:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTAAAAAATGTTGATAAA+AGG | + | chr6.3:74033196-74033215 | None:intergenic | 10.0% |
| ! | AAAATCAAATGTGTACCTTA+TGG | + | chr6.3:74033173-74033192 | None:intergenic | 25.0% |
| !!! | TTATGTTTTGATGACTCTTT+TGG | - | chr6.3:74033233-74033252 | MS.gene05288:intron | 25.0% |
| AAGAGTCATCAAAACATAAG+TGG | + | chr6.3:74033232-74033251 | None:intergenic | 30.0% | |
| TGCAATATCAGATCAACAAA+AGG | - | chr6.3:74033116-74033135 | MS.gene05288:CDS | 30.0% | |
| TTTCCGTGTAATGAAAAAAC+TGG | - | chr6.3:74033367-74033386 | MS.gene05288:CDS | 30.0% | |
| ATATCAGATCAACAAAAGGC+TGG | - | chr6.3:74033120-74033139 | MS.gene05288:CDS | 35.0% | |
| TGGTGAAAAAATGATGCGTT+TGG | - | chr6.3:74033140-74033159 | MS.gene05288:CDS | 35.0% | |
| TTGGATGTTGCAGAAAGAAA+AGG | - | chr6.3:74033256-74033275 | MS.gene05288:intron | 35.0% | |
| !! | GTTTTGATGACTCTTTTGGT+TGG | - | chr6.3:74033237-74033256 | MS.gene05288:intron | 35.0% |
| !!! | CCTCCAGTTTTTTCATTACA+CGG | + | chr6.3:74033373-74033392 | None:intergenic | 35.0% |
| ACTTGATGCAAGACATGCTT+TGG | - | chr6.3:74033316-74033335 | MS.gene05288:CDS | 40.0% | |
| ATTGCATCACTTATCTCGTC+TGG | + | chr6.3:74033102-74033121 | None:intergenic | 40.0% | |
| CCGTGTAATGAAAAAACTGG+AGG | - | chr6.3:74033370-74033389 | MS.gene05288:CDS | 40.0% | |
| TCACAAGAAAATGCATGACC+TGG | - | chr6.3:74033286-74033305 | MS.gene05288:intron | 40.0% | |
| TTGGAGATAGAACAGCTGAA+AGG | - | chr6.3:74033341-74033360 | MS.gene05288:CDS | 40.0% | |
| ! | TTGCATCAAGTCTTTTCACC+AGG | + | chr6.3:74033307-74033326 | None:intergenic | 40.0% |
| !! | TGCAAGACATGCTTTGGAAT+TGG | - | chr6.3:74033322-74033341 | MS.gene05288:CDS | 40.0% |
| GCGTTTGGCTGAAGACCATA+AGG | - | chr6.3:74033155-74033174 | MS.gene05288:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 74033063 | 74033421 | 74033063 | ID=MS.gene05288 |
| chr6.3 | mRNA | 74033063 | 74033421 | 74033063 | ID=MS.gene05288.t1;Parent=MS.gene05288 |
| chr6.3 | exon | 74033308 | 74033421 | 74033308 | ID=MS.gene05288.t1.exon1;Parent=MS.gene05288.t1 |
| chr6.3 | CDS | 74033308 | 74033421 | 74033308 | ID=cds.MS.gene05288.t1;Parent=MS.gene05288.t1 |
| chr6.3 | exon | 74033063 | 74033215 | 74033063 | ID=MS.gene05288.t1.exon2;Parent=MS.gene05288.t1 |
| chr6.3 | CDS | 74033063 | 74033215 | 74033063 | ID=cds.MS.gene05288.t1;Parent=MS.gene05288.t1 |
| Gene Sequence |
| Protein sequence |
