Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36522.t1 | XP_013457381.1 | 98.6 | 218 | 3 | 0 | 1 | 218 | 1 | 218 | 1.40E-123 | 452.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36522.t1 | Q6E2Z6 | 98.2 | 218 | 4 | 0 | 1 | 218 | 1 | 218 | 9.5e-126 | 450.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36522.t1 | A0A072UP31 | 98.6 | 218 | 3 | 0 | 1 | 218 | 1 | 218 | 9.8e-124 | 452.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene26698 | MS.gene36522 | PPI |
| MS.gene030247 | MS.gene36522 | PPI |
| MS.gene24767 | MS.gene36522 | PPI |
| MS.gene012180 | MS.gene36522 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36522.t1 | MTR_4g094720 | 98.624 | 218 | 3 | 0 | 1 | 218 | 1 | 218 | 1.85e-163 | 449 |
| MS.gene36522.t1 | MTR_7g105830 | 31.111 | 180 | 114 | 3 | 6 | 180 | 74 | 248 | 5.35e-23 | 94.0 |
| MS.gene36522.t1 | MTR_1g105090 | 30.000 | 180 | 116 | 3 | 6 | 180 | 76 | 250 | 4.76e-22 | 91.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36522.t1 | AT1G48130 | 72.273 | 220 | 54 | 2 | 1 | 218 | 1 | 215 | 3.08e-116 | 330 |
| MS.gene36522.t1 | AT5G06290 | 31.667 | 180 | 113 | 3 | 6 | 180 | 82 | 256 | 2.45e-24 | 97.4 |
| MS.gene36522.t1 | AT3G11630 | 32.222 | 180 | 112 | 3 | 6 | 180 | 75 | 249 | 1.69e-23 | 95.1 |
| MS.gene36522.t1 | AT5G06290 | 29.448 | 163 | 106 | 2 | 6 | 164 | 82 | 239 | 6.20e-20 | 85.1 |
Find 62 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGCTTAATGATTTCTCTTT+TGG | 0.116285 | 4.1:-17870066 | None:intergenic |
| GATATTTGTACCTTCTTATC+TGG | 0.262678 | 4.1:-17870161 | None:intergenic |
| TCCCCTGGCTTCCAGTTAGC+AGG | 0.316622 | 4.1:-17870360 | None:intergenic |
| ATGTTCCTCCCAGTTGTTGC+AGG | 0.342538 | 4.1:-17870276 | None:intergenic |
| CCATTGGAATCTTTCTCATC+AGG | 0.347454 | 4.1:-17870104 | None:intergenic |
| CCAAGCTCAGTGGTACAAAC+TGG | 0.350619 | 4.1:-17869760 | None:intergenic |
| TTTCTGTACCCTGCAACAAC+TGG | 0.357498 | 4.1:+17870267 | MS.gene36522:CDS |
| CATATCCTTAGCTTGTTCAT+TGG | 0.360719 | 4.1:-17870409 | None:intergenic |
| AAATTGCAACCCCTGCTAAC+TGG | 0.361486 | 4.1:+17870349 | MS.gene36522:CDS |
| TGCAGATCTTCCATCCAAAA+AGG | 0.376713 | 4.1:+17870452 | MS.gene36522:CDS |
| CTTGTGTAGTATCAACTTCA+AGG | 0.408006 | 4.1:-17869666 | None:intergenic |
| GCTTGTTCATTGGTAACATC+AGG | 0.425824 | 4.1:-17870399 | None:intergenic |
| ACAATATGAAGAGCCCTTGA+TGG | 0.428726 | 4.1:-17870134 | None:intergenic |
| ACCCCTGCTAACTGGAAGCC+AGG | 0.430708 | 4.1:+17870357 | MS.gene36522:CDS |
| TACCACTGAGCTTGGTAAGA+TGG | 0.430872 | 4.1:+17869768 | MS.gene36522:CDS |
| CCCCTGGCTTCCAGTTAGCA+GGG | 0.440132 | 4.1:-17870359 | None:intergenic |
| AAACGAGGAGTTATGCTGTT+AGG | 0.461288 | 4.1:+17869814 | MS.gene36522:CDS |
| CAATGGAAATTTACCATCAA+GGG | 0.465608 | 4.1:+17870121 | MS.gene36522:CDS |
| GGATAGTTCACCTTTGCACC+TGG | 0.476765 | 4.1:-17870029 | None:intergenic |
| AATATGCCAGGCCTTACTAT+AGG | 0.481380 | 4.1:+17869628 | None:intergenic |
| CCAATGGAAATTTACCATCA+AGG | 0.485151 | 4.1:+17870120 | MS.gene36522:CDS |
| CCTTGATGGTAAATTTCCAT+TGG | 0.485846 | 4.1:-17870120 | None:intergenic |
| ACCATTCTCTTCTCTCATCC+AGG | 0.497155 | 4.1:+17869727 | MS.gene36522:CDS |
| ATCACTTCTGTTCTGATACA+TGG | 0.498086 | 4.1:+17869704 | MS.gene36522:CDS |
| GTAGTATCAACTTCAAGGTC+AGG | 0.507580 | 4.1:-17869661 | None:intergenic |
| ATCAAGGGCTCTTCATATTG+TGG | 0.511782 | 4.1:+17870136 | MS.gene36522:CDS |
| CTGCAGTCTTAAAACCTTGA+GGG | 0.516211 | 4.1:-17870434 | None:intergenic |
| ACATCAGGAGAAATGACAAC+AGG | 0.519996 | 4.1:-17870384 | None:intergenic |
| CAAGCTCAGTGGTACAAACT+GGG | 0.522401 | 4.1:-17869759 | None:intergenic |
| TCAAGGGCTCTTCATATTGT+GGG | 0.523725 | 4.1:+17870137 | MS.gene36522:CDS |
| TGTTCCTCCCAGTTGTTGCA+GGG | 0.527210 | 4.1:-17870275 | None:intergenic |
| TTCTGTACCCTGCAACAACT+GGG | 0.528210 | 4.1:+17870268 | MS.gene36522:CDS |
| GAATTGTGTCACCTATAGTA+AGG | 0.528944 | 4.1:-17869639 | None:intergenic |
| CCAGTTTGTACCACTGAGCT+TGG | 0.529958 | 4.1:+17869760 | MS.gene36522:CDS |
| TCAAAACTAGAAAATATGCC+AGG | 0.532876 | 4.1:+17869616 | None:intergenic |
| CAAACTGGGGTGAAATCACC+TGG | 0.538828 | 4.1:-17869745 | None:intergenic |
| ATCTAGAATCTCACAAGGAG+TGG | 0.546589 | 4.1:+17869851 | MS.gene36522:CDS |
| CCCCTGCTAACTGGAAGCCA+GGG | 0.547614 | 4.1:+17870358 | MS.gene36522:CDS |
| GTGTCACCTATAGTAAGGCC+TGG | 0.556059 | 4.1:-17869634 | None:intergenic |
| CCCTGGCTTCCAGTTAGCAG+GGG | 0.564511 | 4.1:-17870358 | None:intergenic |
| ATGTATCAGAACAGAAGTGA+TGG | 0.570051 | 4.1:-17869702 | None:intergenic |
| TGTTACCAATGAACAAGCTA+AGG | 0.572171 | 4.1:+17870404 | MS.gene36522:CDS |
| TATTGTGGGACCAGATAAGA+AGG | 0.572882 | 4.1:+17870151 | MS.gene36522:CDS |
| GAAATGACAACAGGTTCCCC+TGG | 0.579339 | 4.1:-17870375 | None:intergenic |
| GTAAATGCAGCCAGGTGCAA+AGG | 0.582844 | 4.1:+17870019 | MS.gene36522:intron |
| TGCAACAACTGGGAGGAACA+TGG | 0.585961 | 4.1:+17870278 | MS.gene36522:CDS |
| CCTGATGAGAAAGATTCCAA+TGG | 0.589101 | 4.1:+17870104 | MS.gene36522:CDS |
| TCTGCAGTCTTAAAACCTTG+AGG | 0.590174 | 4.1:-17870435 | None:intergenic |
| GCTAAGGATATGTTCCCTCA+AGG | 0.596047 | 4.1:+17870420 | MS.gene36522:CDS |
| ACCTGGATGAGAGAAGAGAA+TGG | 0.604374 | 4.1:-17869728 | None:intergenic |
| GCAGGCACATTATCAAACAT+TGG | 0.624118 | 4.1:-17870490 | None:intergenic |
| GAACATGGATGAAGTGCTGA+GGG | 0.626828 | 4.1:+17870293 | MS.gene36522:CDS |
| AATCATTAAGCAACTCAACA+TGG | 0.634966 | 4.1:+17870076 | MS.gene36522:CDS |
| GGAACATGGATGAAGTGCTG+AGG | 0.641851 | 4.1:+17870292 | MS.gene36522:CDS |
| CTTGAAGTTGATACTACACA+AGG | 0.656492 | 4.1:+17869667 | MS.gene36522:CDS |
| GCTTCTGAGTTTAACAAACG+AGG | 0.664933 | 4.1:+17869799 | MS.gene36522:CDS |
| AGCCATCTTACCAAGCTCAG+TGG | 0.696126 | 4.1:-17869770 | None:intergenic |
| CCCTGCTAACTGGAAGCCAG+GGG | 0.697213 | 4.1:+17870359 | MS.gene36522:CDS |
| CATGGATGAAGTGCTGAGGG+TGG | 0.707751 | 4.1:+17870296 | MS.gene36522:CDS |
| TGATGATCTAGAATCTCACA+AGG | 0.709676 | 4.1:+17869846 | MS.gene36522:CDS |
| AAGCTCAGTGGTACAAACTG+GGG | 0.712192 | 4.1:-17869758 | None:intergenic |
| TGTACCCTGCAACAACTGGG+AGG | 0.722121 | 4.1:+17870271 | MS.gene36522:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAAAATAGGAAAACTTA+CGG | - | chr4.1:17869899-17869918 | None:intergenic | 20.0% |
| !! | ACAAATTAAACCATCATTTA+TGG | - | chr4.1:17870230-17870249 | None:intergenic | 20.0% |
| !! | GAAACATGCATAGAAAATTT+TGG | - | chr4.1:17869938-17869957 | None:intergenic | 25.0% |
| !! | TAAGAAGGTACAAATATCTA+TGG | + | chr4.1:17870166-17870185 | MS.gene36522:intron | 25.0% |
| !! | TGCTTAATGATTTCTCTTTT+GGG | - | chr4.1:17870068-17870087 | None:intergenic | 25.0% |
| !! | TTGCTTAATGATTTCTCTTT+TGG | - | chr4.1:17870069-17870088 | None:intergenic | 25.0% |
| !!! | CTTTTTTCTTCCATAAATGA+TGG | + | chr4.1:17870217-17870236 | MS.gene36522:intron | 25.0% |
| AATCATTAAGCAACTCAACA+TGG | + | chr4.1:17870076-17870095 | MS.gene36522:CDS | 30.0% | |
| ATGCATGTGGAAAAGAAAAT+AGG | - | chr4.1:17869910-17869929 | None:intergenic | 30.0% | |
| CAATGGAAATTTACCATCAA+GGG | + | chr4.1:17870121-17870140 | MS.gene36522:CDS | 30.0% | |
| GATATTTGTACCTTCTTATC+TGG | - | chr4.1:17870164-17870183 | None:intergenic | 30.0% | |
| !!! | CTTTTGGGATCACTAATTAT+TGG | - | chr4.1:17870053-17870072 | None:intergenic | 30.0% |
| !!! | TTTTTTATGTAAATGCAGCC+AGG | + | chr4.1:17870011-17870030 | MS.gene36522:intron | 30.0% |
| ATCACTTCTGTTCTGATACA+TGG | + | chr4.1:17869704-17869723 | MS.gene36522:CDS | 35.0% | |
| ATGTATCAGAACAGAAGTGA+TGG | - | chr4.1:17869705-17869724 | None:intergenic | 35.0% | |
| CCAATGGAAATTTACCATCA+AGG | + | chr4.1:17870120-17870139 | MS.gene36522:CDS | 35.0% | |
| CTTGTGTAGTATCAACTTCA+AGG | - | chr4.1:17869669-17869688 | None:intergenic | 35.0% | |
| GAATTGTGTCACCTATAGTA+AGG | - | chr4.1:17869642-17869661 | None:intergenic | 35.0% | |
| TGTTACCAATGAACAAGCTA+AGG | + | chr4.1:17870404-17870423 | MS.gene36522:CDS | 35.0% | |
| ! | CCTTGATGGTAAATTTCCAT+TGG | - | chr4.1:17870123-17870142 | None:intergenic | 35.0% |
| ! | CTAAGATACTCCTTTTTGGA+TGG | - | chr4.1:17870465-17870484 | None:intergenic | 35.0% |
| ! | GAACCTAAGATACTCCTTTT+TGG | - | chr4.1:17870469-17870488 | None:intergenic | 35.0% |
| ! | TGATGATCTAGAATCTCACA+AGG | + | chr4.1:17869846-17869865 | MS.gene36522:CDS | 35.0% |
| !! | AATTTTGGTATGCATGCATG+TGG | - | chr4.1:17869923-17869942 | None:intergenic | 35.0% |
| !! | CATATCCTTAGCTTGTTCAT+TGG | - | chr4.1:17870412-17870431 | None:intergenic | 35.0% |
| !! | CATCCAAAAAGGAGTATCTT+AGG | + | chr4.1:17870463-17870482 | MS.gene36522:CDS | 35.0% |
| !! | CTTGAAGTTGATACTACACA+AGG | + | chr4.1:17869667-17869686 | MS.gene36522:CDS | 35.0% |
| AAACGAGGAGTTATGCTGTT+AGG | + | chr4.1:17869814-17869833 | MS.gene36522:CDS | 40.0% | |
| ACAATATGAAGAGCCCTTGA+TGG | - | chr4.1:17870137-17870156 | None:intergenic | 40.0% | |
| ACATCAGGAGAAATGACAAC+AGG | - | chr4.1:17870387-17870406 | None:intergenic | 40.0% | |
| ATCAAGGGCTCTTCATATTG+TGG | + | chr4.1:17870136-17870155 | MS.gene36522:CDS | 40.0% | |
| CCATTGGAATCTTTCTCATC+AGG | - | chr4.1:17870107-17870126 | None:intergenic | 40.0% | |
| CCTGATGAGAAAGATTCCAA+TGG | + | chr4.1:17870104-17870123 | MS.gene36522:CDS | 40.0% | |
| CTGCAGTCTTAAAACCTTGA+GGG | - | chr4.1:17870437-17870456 | None:intergenic | 40.0% | |
| GCTTCTGAGTTTAACAAACG+AGG | + | chr4.1:17869799-17869818 | MS.gene36522:CDS | 40.0% | |
| GTAGTATCAACTTCAAGGTC+AGG | - | chr4.1:17869664-17869683 | None:intergenic | 40.0% | |
| TATTGTGGGACCAGATAAGA+AGG | + | chr4.1:17870151-17870170 | MS.gene36522:CDS | 40.0% | |
| TCAAGGGCTCTTCATATTGT+GGG | + | chr4.1:17870137-17870156 | MS.gene36522:CDS | 40.0% | |
| TCTGCAGTCTTAAAACCTTG+AGG | - | chr4.1:17870438-17870457 | None:intergenic | 40.0% | |
| TGCAGATCTTCCATCCAAAA+AGG | + | chr4.1:17870452-17870471 | MS.gene36522:CDS | 40.0% | |
| ! | ATCTAGAATCTCACAAGGAG+TGG | + | chr4.1:17869851-17869870 | MS.gene36522:CDS | 40.0% |
| ! | GCTTGTTCATTGGTAACATC+AGG | - | chr4.1:17870402-17870421 | None:intergenic | 40.0% |
| AAATTGCAACCCCTGCTAAC+TGG | + | chr4.1:17870349-17870368 | MS.gene36522:CDS | 45.0% | |
| AAGCTCAGTGGTACAAACTG+GGG | - | chr4.1:17869761-17869780 | None:intergenic | 45.0% | |
| ACCATTCTCTTCTCTCATCC+AGG | + | chr4.1:17869727-17869746 | MS.gene36522:CDS | 45.0% | |
| ACCTGGATGAGAGAAGAGAA+TGG | - | chr4.1:17869731-17869750 | None:intergenic | 45.0% | |
| CAAGCTCAGTGGTACAAACT+GGG | - | chr4.1:17869762-17869781 | None:intergenic | 45.0% | |
| GCTAAGGATATGTTCCCTCA+AGG | + | chr4.1:17870420-17870439 | MS.gene36522:CDS | 45.0% | |
| TACCACTGAGCTTGGTAAGA+TGG | + | chr4.1:17869768-17869787 | MS.gene36522:CDS | 45.0% | |
| TTCTGTACCCTGCAACAACT+GGG | + | chr4.1:17870268-17870287 | MS.gene36522:CDS | 45.0% | |
| TTTCTGTACCCTGCAACAAC+TGG | + | chr4.1:17870267-17870286 | MS.gene36522:CDS | 45.0% | |
| !! | GAACATGGATGAAGTGCTGA+GGG | + | chr4.1:17870293-17870312 | MS.gene36522:CDS | 45.0% |
| !!! | ATTGATAATATTAATTAATT+AGG | + | chr4.1:17869981-17870000 | MS.gene36522:intron | 5.0% |
| AGCCATCTTACCAAGCTCAG+TGG | - | chr4.1:17869773-17869792 | None:intergenic | 50.0% | |
| ATGTTCCTCCCAGTTGTTGC+AGG | - | chr4.1:17870279-17870298 | None:intergenic | 50.0% | |
| CAAACTGGGGTGAAATCACC+TGG | - | chr4.1:17869748-17869767 | None:intergenic | 50.0% | |
| CCAAGCTCAGTGGTACAAAC+TGG | - | chr4.1:17869763-17869782 | None:intergenic | 50.0% | |
| CCAGTTTGTACCACTGAGCT+TGG | + | chr4.1:17869760-17869779 | MS.gene36522:CDS | 50.0% | |
| GAAATGACAACAGGTTCCCC+TGG | - | chr4.1:17870378-17870397 | None:intergenic | 50.0% | |
| GGATAGTTCACCTTTGCACC+TGG | - | chr4.1:17870032-17870051 | None:intergenic | 50.0% | |
| GTAAATGCAGCCAGGTGCAA+AGG | + | chr4.1:17870019-17870038 | MS.gene36522:intron | 50.0% | |
| GTGTCACCTATAGTAAGGCC+TGG | - | chr4.1:17869637-17869656 | None:intergenic | 50.0% | |
| TGCAACAACTGGGAGGAACA+TGG | + | chr4.1:17870278-17870297 | MS.gene36522:CDS | 50.0% | |
| TGTTCCTCCCAGTTGTTGCA+GGG | - | chr4.1:17870278-17870297 | None:intergenic | 50.0% | |
| !! | GGAACATGGATGAAGTGCTG+AGG | + | chr4.1:17870292-17870311 | MS.gene36522:CDS | 50.0% |
| TGTACCCTGCAACAACTGGG+AGG | + | chr4.1:17870271-17870290 | MS.gene36522:CDS | 55.0% | |
| !! | CATGGATGAAGTGCTGAGGG+TGG | + | chr4.1:17870296-17870315 | MS.gene36522:CDS | 55.0% |
| ACCCCTGCTAACTGGAAGCC+AGG | + | chr4.1:17870357-17870376 | MS.gene36522:CDS | 60.0% | |
| CCCCTGCTAACTGGAAGCCA+GGG | + | chr4.1:17870358-17870377 | MS.gene36522:CDS | 60.0% | |
| CCCCTGGCTTCCAGTTAGCA+GGG | - | chr4.1:17870362-17870381 | None:intergenic | 60.0% | |
| CCCTGCTAACTGGAAGCCAG+GGG | + | chr4.1:17870359-17870378 | MS.gene36522:CDS | 60.0% | |
| CCCTGGCTTCCAGTTAGCAG+GGG | - | chr4.1:17870361-17870380 | None:intergenic | 60.0% | |
| TCCCCTGGCTTCCAGTTAGC+AGG | - | chr4.1:17870363-17870382 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 17869631 | 17870500 | 17869631 | ID=MS.gene36522 |
| chr4.1 | mRNA | 17869631 | 17870500 | 17869631 | ID=MS.gene36522.t1;Parent=MS.gene36522 |
| chr4.1 | exon | 17869631 | 17869897 | 17869631 | ID=MS.gene36522.t1.exon1;Parent=MS.gene36522.t1 |
| chr4.1 | CDS | 17869631 | 17869897 | 17869631 | ID=cds.MS.gene36522.t1;Parent=MS.gene36522.t1 |
| chr4.1 | exon | 17870029 | 17870172 | 17870029 | ID=MS.gene36522.t1.exon2;Parent=MS.gene36522.t1 |
| chr4.1 | CDS | 17870029 | 17870172 | 17870029 | ID=cds.MS.gene36522.t1;Parent=MS.gene36522.t1 |
| chr4.1 | exon | 17870255 | 17870500 | 17870255 | ID=MS.gene36522.t1.exon3;Parent=MS.gene36522.t1 |
| chr4.1 | CDS | 17870255 | 17870500 | 17870255 | ID=cds.MS.gene36522.t1;Parent=MS.gene36522.t1 |
| Gene Sequence |
| Protein sequence |
