Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36889.t1 | RHN40044.1 | 67.9 | 296 | 69 | 10 | 1 | 290 | 1 | 276 | 4.90E-100 | 374.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36889.t1 | Q7M443 | 35.0 | 274 | 151 | 9 | 14 | 280 | 2 | 255 | 6.4e-32 | 139.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36889.t1 | A0A396GKD7 | 67.9 | 296 | 69 | 10 | 1 | 290 | 1 | 276 | 3.5e-100 | 374.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049681 | MS.gene36889 | 0.802504 | 5.44E-49 | -1.69E-46 |
| MS.gene049993 | MS.gene36889 | 0.800719 | 1.26E-48 | -1.69E-46 |
| MS.gene051860 | MS.gene36889 | 0.832272 | 1.04E-55 | -1.69E-46 |
| MS.gene054183 | MS.gene36889 | 0.810622 | 1.05E-50 | -1.69E-46 |
| MS.gene059294 | MS.gene36889 | 0.814497 | 1.49E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36889.t1 | MTR_0002s0040 | 53.698 | 311 | 103 | 10 | 1 | 290 | 1 | 291 | 3.51e-95 | 283 |
| MS.gene36889.t1 | MTR_0263s0020 | 56.164 | 292 | 96 | 9 | 1 | 280 | 1 | 272 | 5.77e-94 | 280 |
| MS.gene36889.t1 | MTR_1457s0010 | 66.522 | 230 | 53 | 8 | 65 | 290 | 1 | 210 | 3.13e-93 | 275 |
| MS.gene36889.t1 | MTR_0002s1060 | 51.546 | 291 | 108 | 11 | 4 | 280 | 6 | 277 | 4.62e-75 | 232 |
| MS.gene36889.t1 | MTR_0062s0020 | 38.542 | 288 | 140 | 8 | 14 | 280 | 4 | 275 | 1.80e-48 | 164 |
| MS.gene36889.t1 | MTR_0062s0090 | 39.510 | 286 | 138 | 10 | 14 | 280 | 4 | 273 | 8.54e-48 | 162 |
| MS.gene36889.t1 | MTR_0062s0040 | 39.510 | 286 | 138 | 10 | 14 | 280 | 4 | 273 | 8.54e-48 | 162 |
| MS.gene36889.t1 | MTR_0062s0060 | 39.510 | 286 | 138 | 10 | 14 | 280 | 4 | 273 | 8.54e-48 | 162 |
| MS.gene36889.t1 | MTR_0443s0040 | 39.175 | 291 | 132 | 12 | 14 | 280 | 16 | 285 | 1.49e-47 | 161 |
| MS.gene36889.t1 | MTR_0062s0170 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 9.10e-39 | 139 |
| MS.gene36889.t1 | MTR_0062s0110 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 9.10e-39 | 139 |
| MS.gene36889.t1 | MTR_0062s0130 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 9.10e-39 | 139 |
| MS.gene36889.t1 | MTR_2g036240 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 9.10e-39 | 139 |
| MS.gene36889.t1 | MTR_2g036260 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 9.10e-39 | 139 |
| MS.gene36889.t1 | MTR_2g036290 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 9.10e-39 | 139 |
| MS.gene36889.t1 | MTR_8g046730 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 9.10e-39 | 139 |
| MS.gene36889.t1 | MTR_6g079630 | 34.307 | 274 | 155 | 8 | 13 | 280 | 33 | 287 | 3.41e-37 | 134 |
| MS.gene36889.t1 | MTR_2g096030 | 32.990 | 291 | 163 | 10 | 14 | 291 | 31 | 302 | 1.38e-33 | 125 |
| MS.gene36889.t1 | MTR_2g096040 | 33.202 | 253 | 145 | 6 | 39 | 283 | 26 | 262 | 1.65e-28 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATATAAAAGATTTTGGGTA+TGG | - | 57517:8005-8024 | MS.gene36889:intergenic | 20.0% |
| !! | CAGGGAATATAAAAGATTTT+GGG | - | 57517:8010-8029 | MS.gene36889:intergenic | 25.0% |
| !! | TCAGGGAATATAAAAGATTT+TGG | - | 57517:8011-8030 | MS.gene36889:intergenic | 25.0% |
| !! | TTCATTTTACGAGAATACAT+TGG | + | 57517:7972-7991 | MS.gene36889:CDS | 25.0% |
| AGAGAAAGGATATTTAGTAG+GGG | - | 57517:8264-8283 | MS.gene36889:intergenic | 30.0% | |
| ATTTCGTTGTAGTCAATATC+AGG | - | 57517:8029-8048 | MS.gene36889:intergenic | 30.0% | |
| GAGAGAAAGGATATTTAGTA+GGG | - | 57517:8265-8284 | MS.gene36889:intergenic | 30.0% | |
| TATAAAAAGACTCAAGGAAG+AGG | + | 57517:8427-8446 | MS.gene36889:CDS | 30.0% | |
| TTTCGTTGTAGTCAATATCA+GGG | - | 57517:8028-8047 | MS.gene36889:intergenic | 30.0% | |
| ! | GTAGTGTTCATTAGTTTCAT+GGG | - | 57517:8483-8502 | MS.gene36889:intergenic | 30.0% |
| ! | TGTAGTGTTCATTAGTTTCA+TGG | - | 57517:8484-8503 | MS.gene36889:intergenic | 30.0% |
| !! | AATCGATTTCCATGTCATTT+TGG | + | 57517:8046-8065 | MS.gene36889:CDS | 30.0% |
| !! | ACTTTTGAAAAACTCCTAGT+AGG | + | 57517:8632-8651 | MS.gene36889:CDS | 30.0% |
| !!! | TCTTTTTCAATAGGAGAGAA+AGG | - | 57517:8278-8297 | MS.gene36889:intergenic | 30.0% |
| AAGATTTCTCCAACTACGTT+GGG | + | 57517:8399-8418 | MS.gene36889:CDS | 35.0% | |
| ACCACAGTGACGATATTAAT+TGG | + | 57517:8519-8538 | MS.gene36889:CDS | 35.0% | |
| ACTTACAACGAAGATGGAAA+AGG | + | 57517:8083-8102 | MS.gene36889:CDS | 35.0% | |
| ATCTTCGTTGTAAGTCTCAT+GGG | - | 57517:8078-8097 | MS.gene36889:intergenic | 35.0% | |
| CCACAGTGACGATATTAATT+GGG | + | 57517:8520-8539 | MS.gene36889:CDS | 35.0% | |
| CTCCTATTGAAAAAGAATCC+TGG | + | 57517:8282-8301 | MS.gene36889:CDS | 35.0% | |
| GAGAAAGGATATTTAGTAGG+GGG | - | 57517:8263-8282 | MS.gene36889:intergenic | 35.0% | |
| GGACGTTATAAAAAGACTCA+AGG | + | 57517:8421-8440 | MS.gene36889:CDS | 35.0% | |
| GGAGAGAAAGGATATTTAGT+AGG | - | 57517:8266-8285 | MS.gene36889:intergenic | 35.0% | |
| GTTGGAGAAATCTTCTTCAT+TGG | - | 57517:8393-8412 | MS.gene36889:intergenic | 35.0% | |
| ! | TGTTCATTAGTTTCATGGGA+TGG | - | 57517:8479-8498 | MS.gene36889:intergenic | 35.0% |
| !!! | CACCAGGATTCTTTTTCAAT+AGG | - | 57517:8287-8306 | MS.gene36889:intergenic | 35.0% |
| AAAAGACTCAAGGAAGAGGT+AGG | + | 57517:8431-8450 | MS.gene36889:CDS | 40.0% | |
| AACACTACAAGCTACTCTAC+AGG | + | 57517:8495-8514 | MS.gene36889:CDS | 40.0% | |
| ACACTACAAGCTACTCTACA+GGG | + | 57517:8496-8515 | MS.gene36889:CDS | 40.0% | |
| AGATTTCTCCAACTACGTTG+GGG | + | 57517:8400-8419 | MS.gene36889:CDS | 40.0% | |
| ATCCAAGACTACACGTTTGA+TGG | + | 57517:8338-8357 | MS.gene36889:CDS | 40.0% | |
| CAGATATCAAGATCCCAATC+GGG | - | 57517:8125-8144 | MS.gene36889:intergenic | 40.0% | |
| CATCTTCGTTGTAAGTCTCA+TGG | - | 57517:8079-8098 | MS.gene36889:intergenic | 40.0% | |
| CATGAGACTTACAACGAAGA+TGG | + | 57517:8077-8096 | MS.gene36889:CDS | 40.0% | |
| CCCAATTAATATCGTCACTG+TGG | - | 57517:8523-8542 | MS.gene36889:intergenic | 40.0% | |
| CGGAAAATTCTATCCCGATT+GGG | + | 57517:8109-8128 | MS.gene36889:CDS | 40.0% | |
| GAAGATTTCTCCAACTACGT+TGG | + | 57517:8398-8417 | MS.gene36889:CDS | 40.0% | |
| GAATCGATCAACAGTGTTAC+CGG | - | 57517:7955-7974 | MS.gene36889:intergenic | 40.0% | |
| GATCTATGTTAGACTCGCAA+GGG | + | 57517:8601-8620 | MS.gene36889:CDS | 40.0% | |
| GATTGGGATCTTGATATCTG+CGG | + | 57517:8125-8144 | MS.gene36889:CDS | 40.0% | |
| GCAGATATCAAGATCCCAAT+CGG | - | 57517:8126-8145 | MS.gene36889:intergenic | 40.0% | |
| TATCAAGCTCAAGTATCCCA+AGG | + | 57517:8166-8185 | MS.gene36889:CDS | 40.0% | |
| TCGTGATATACAAAGTGTGC+AGG | + | 57517:8214-8233 | MS.gene36889:CDS | 40.0% | |
| TGATCTATGTTAGACTCGCA+AGG | + | 57517:8600-8619 | MS.gene36889:CDS | 40.0% | |
| TTGCGAGTCTAACATAGATC+AGG | - | 57517:8601-8620 | MS.gene36889:intergenic | 40.0% | |
| ! | AACGCAGTGGCATTTTCATT+TGG | + | 57517:8741-8760 | MS.gene36889:CDS | 40.0% |
| ! | CCAATCGGGATAGAATTTTC+CGG | - | 57517:8111-8130 | MS.gene36889:intergenic | 40.0% |
| !! | GATCTTTTTGAGCGAATCGA+CGG | - | 57517:8318-8337 | MS.gene36889:intergenic | 40.0% |
| ACAAAGTCAGTCTCGTCAGT+GGG | - | 57517:8575-8594 | MS.gene36889:intergenic | 45.0% | |
| ACGAAGACATCTAGTGGAAG+TGG | - | 57517:8692-8711 | MS.gene36889:intergenic | 45.0% | |
| AGTTTCATGGGATGGAGCAA+TGG | - | 57517:8471-8490 | MS.gene36889:intergenic | 45.0% | |
| CCGGAAAATTCTATCCCGAT+TGG | + | 57517:8108-8127 | MS.gene36889:CDS | 45.0% | |
| CTAAGTACCACCTTGACCTT+GGG | - | 57517:8185-8204 | MS.gene36889:intergenic | 45.0% | |
| GCAGGATATCATCAAGCACA+AGG | + | 57517:8232-8251 | MS.gene36889:CDS | 45.0% | |
| GCGTTGAACCTTCATGAGAT+CGG | - | 57517:8726-8745 | MS.gene36889:intergenic | 45.0% | |
| GGAAGAGGTAGGTATCAATG+TGG | + | 57517:8442-8461 | MS.gene36889:CDS | 45.0% | |
| GGTTAGGCTGCATGTAGAAT+TGG | - | 57517:8553-8572 | MS.gene36889:intergenic | 45.0% | |
| GTCAAGGTGGTACTTAGCAT+TGG | + | 57517:8188-8207 | MS.gene36889:CDS | 45.0% | |
| GTTGTAAGTCTCATGGGCAA+AGG | - | 57517:8072-8091 | MS.gene36889:intergenic | 45.0% | |
| TACAAAGTCAGTCTCGTCAG+TGG | - | 57517:8576-8595 | MS.gene36889:intergenic | 45.0% | |
| TATAACGTCCCCAACGTAGT+TGG | - | 57517:8411-8430 | MS.gene36889:intergenic | 45.0% | |
| TGCCATCAAACGTGTAGTCT+TGG | - | 57517:8343-8362 | MS.gene36889:intergenic | 45.0% | |
| ! | AACGAAGATGGAAAAGGCAC+CGG | + | 57517:8089-8108 | MS.gene36889:CDS | 45.0% |
| ! | AAGGTGGTACTTAGCATTGG+TGG | + | 57517:8191-8210 | MS.gene36889:CDS | 45.0% |
| AGCTTGATATCCTGCACACG+TGG | - | 57517:8155-8174 | MS.gene36889:intergenic | 50.0% | |
| ATCGACGGCATTATCACACC+AGG | - | 57517:8303-8322 | MS.gene36889:intergenic | 50.0% | |
| ATTACGTCCGTCACGTGGAT+CGG | - | 57517:8666-8685 | MS.gene36889:intergenic | 50.0% | |
| CAAAGTCAGTCTCGTCAGTG+GGG | - | 57517:8574-8593 | MS.gene36889:intergenic | 50.0% | |
| CAAGTATCCCAAGGTCAAGG+TGG | + | 57517:8175-8194 | MS.gene36889:CDS | 50.0% | |
| CTCATGAAGGTTCAACGCAG+TGG | + | 57517:8728-8747 | MS.gene36889:CDS | 50.0% | |
| CTCGCAACGAAGACATCTAG+TGG | - | 57517:8698-8717 | MS.gene36889:intergenic | 50.0% | |
| GAGTTGCTCCGATCTCATGA+AGG | + | 57517:8715-8734 | MS.gene36889:CDS | 50.0% | |
| GCTAAGTACCACCTTGACCT+TGG | - | 57517:8186-8205 | MS.gene36889:intergenic | 50.0% | |
| GCTCAAGTATCCCAAGGTCA+AGG | + | 57517:8172-8191 | MS.gene36889:CDS | 50.0% | |
| GGGCAAAGGCCAAAATGACA+TGG | - | 57517:8058-8077 | MS.gene36889:intergenic | 50.0% | |
| GGAACATTACGTCCGTCACG+TGG | - | 57517:8671-8690 | MS.gene36889:intergenic | 55.0% | |
| TCAGTCTCGTCAGTGGGGTT+AGG | - | 57517:8569-8588 | MS.gene36889:intergenic | 55.0% | |
| ! | GATCGGTGCTTGCTCCTACT+AGG | - | 57517:8649-8668 | MS.gene36889:intergenic | 55.0% |
| CTGCGGTATACCACGTGTGC+AGG | + | 57517:8142-8161 | MS.gene36889:CDS | 60.0% | |
| !! | GCAAGCACCGATCCACGTGA+CGG | + | 57517:8656-8675 | MS.gene36889:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57517 | gene | 7936 | 8826 | 7936 | ID=MS.gene36889 |
| 57517 | mRNA | 7936 | 8826 | 7936 | ID=MS.gene36889.t1;Parent=MS.gene36889 |
| 57517 | exon | 7936 | 8826 | 7936 | ID=MS.gene36889.t1.exon1;Parent=MS.gene36889.t1 |
| 57517 | CDS | 7936 | 8826 | 7936 | ID=cds.MS.gene36889.t1;Parent=MS.gene36889.t1 |
| Gene Sequence |
| Protein sequence |
