Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36953.t1 | XP_003627005.1 | 99.8 | 832 | 2 | 0 | 1 | 832 | 1 | 832 | 0.00E+00 | 1680.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36953.t1 | Q9ZU11 | 83.7 | 838 | 127 | 5 | 1 | 832 | 3 | 836 | 0.0e+00 | 1421.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36953.t1 | G7LFY5 | 99.8 | 832 | 2 | 0 | 1 | 832 | 1 | 832 | 0.0e+00 | 1680.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene36953.t1 | TF | HB-HD-ZIP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene36953 | MS.gene014625 | PPI |
| MS.gene36953 | MS.gene031245 | PPI |
| MS.gene36953 | MS.gene40278 | PPI |
| MS.gene36953 | MS.gene40724 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36953.t1 | MTR_8g013980 | 99.760 | 832 | 2 | 0 | 1 | 832 | 1 | 832 | 0.0 | 1730 |
| MS.gene36953.t1 | MTR_2g101190 | 88.650 | 837 | 89 | 4 | 1 | 832 | 1 | 836 | 0.0 | 1557 |
| MS.gene36953.t1 | MTR_3g109800 | 81.220 | 836 | 145 | 5 | 2 | 832 | 7 | 835 | 0.0 | 1434 |
| MS.gene36953.t1 | MTR_3g109800 | 81.091 | 788 | 137 | 5 | 2 | 784 | 7 | 787 | 0.0 | 1339 |
| MS.gene36953.t1 | MTR_3g109800 | 80.797 | 703 | 125 | 4 | 2 | 699 | 7 | 704 | 0.0 | 1194 |
| MS.gene36953.t1 | MTR_2g030130 | 66.187 | 834 | 264 | 9 | 11 | 832 | 9 | 836 | 0.0 | 1149 |
| MS.gene36953.t1 | MTR_4g058970 | 64.397 | 837 | 276 | 10 | 7 | 832 | 21 | 846 | 0.0 | 1108 |
| MS.gene36953.t1 | MTR_2g094520 | 59.952 | 839 | 307 | 11 | 8 | 832 | 16 | 839 | 0.0 | 1038 |
| MS.gene36953.t1 | MTR_3g109800 | 79.195 | 596 | 121 | 2 | 238 | 832 | 1 | 594 | 0.0 | 1013 |
| MS.gene36953.t1 | MTR_8g432540 | 21.912 | 502 | 299 | 17 | 195 | 667 | 369 | 806 | 5.96e-14 | 76.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36953.t1 | AT1G52150 | 83.652 | 838 | 127 | 5 | 1 | 832 | 3 | 836 | 0.0 | 1469 |
| MS.gene36953.t1 | AT1G52150 | 83.552 | 839 | 127 | 6 | 1 | 832 | 3 | 837 | 0.0 | 1464 |
| MS.gene36953.t1 | AT1G52150 | 83.165 | 790 | 123 | 5 | 1 | 784 | 3 | 788 | 0.0 | 1368 |
| MS.gene36953.t1 | AT4G32880 | 77.197 | 842 | 173 | 9 | 1 | 832 | 1 | 833 | 0.0 | 1354 |
| MS.gene36953.t1 | AT2G34710 | 65.077 | 839 | 269 | 13 | 11 | 832 | 21 | 852 | 0.0 | 1104 |
| MS.gene36953.t1 | AT1G30490 | 63.294 | 850 | 279 | 14 | 1 | 832 | 7 | 841 | 0.0 | 1081 |
| MS.gene36953.t1 | AT5G60690 | 63.095 | 840 | 282 | 10 | 7 | 832 | 17 | 842 | 0.0 | 1079 |
| MS.gene36953.t1 | AT5G32670 | 63.514 | 74 | 27 | 0 | 281 | 354 | 37 | 110 | 9.86e-25 | 102 |
| MS.gene36953.t1 | AT4G00730 | 23.333 | 540 | 309 | 19 | 15 | 475 | 137 | 650 | 2.86e-19 | 93.6 |
| MS.gene36953.t1 | AT4G04890 | 23.413 | 504 | 286 | 20 | 196 | 668 | 302 | 736 | 1.33e-17 | 88.2 |
| MS.gene36953.t1 | AT4G04890 | 23.413 | 504 | 286 | 20 | 196 | 668 | 302 | 736 | 1.33e-17 | 88.2 |
| MS.gene36953.t1 | AT1G05230 | 21.415 | 509 | 290 | 15 | 189 | 668 | 288 | 715 | 4.08e-13 | 73.6 |
| MS.gene36953.t1 | AT1G05230 | 21.218 | 509 | 293 | 15 | 189 | 668 | 288 | 717 | 2.03e-12 | 71.6 |
| MS.gene36953.t1 | AT1G05230 | 21.218 | 509 | 293 | 15 | 189 | 668 | 288 | 717 | 2.03e-12 | 71.6 |
| MS.gene36953.t1 | AT1G05230 | 21.218 | 509 | 293 | 15 | 189 | 668 | 288 | 717 | 2.03e-12 | 71.6 |
| MS.gene36953.t1 | AT1G05230 | 21.218 | 509 | 293 | 15 | 189 | 668 | 288 | 717 | 2.03e-12 | 71.6 |
| MS.gene36953.t1 | AT1G05230 | 21.218 | 509 | 293 | 15 | 189 | 668 | 288 | 717 | 2.03e-12 | 71.6 |
| MS.gene36953.t1 | AT1G05230 | 21.218 | 509 | 293 | 15 | 189 | 668 | 288 | 717 | 2.03e-12 | 71.6 |
| MS.gene36953.t1 | AT1G05230 | 21.218 | 509 | 293 | 15 | 189 | 668 | 288 | 717 | 2.03e-12 | 71.6 |
| MS.gene36953.t1 | AT1G05230 | 21.218 | 509 | 293 | 15 | 189 | 668 | 288 | 717 | 2.03e-12 | 71.6 |
| MS.gene36953.t1 | AT1G05230 | 21.218 | 509 | 293 | 15 | 189 | 668 | 288 | 717 | 2.03e-12 | 71.6 |
| MS.gene36953.t1 | AT1G05230 | 21.218 | 509 | 293 | 15 | 189 | 668 | 288 | 717 | 2.03e-12 | 71.6 |
| MS.gene36953.t1 | AT1G73360 | 28.287 | 251 | 135 | 10 | 15 | 237 | 35 | 268 | 6.98e-11 | 66.6 |
| MS.gene36953.t1 | AT3G61150 | 31.387 | 137 | 71 | 4 | 7 | 143 | 105 | 218 | 8.01e-11 | 66.2 |
Find 227 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCGAAGACAACAACTTATT+AGG | 0.174771 | 8.4:-76974255 | MS.gene36953:CDS |
| GCTACAAGGGCCAACTTTAA+TGG | 0.244448 | 8.4:+76970301 | None:intergenic |
| TGGCACCTGCTCGCGACTTC+TGG | 0.256710 | 8.4:-76971927 | MS.gene36953:CDS |
| AGCAGAGAAAAGAGTCTTTC+AGG | 0.266533 | 8.4:-76973469 | MS.gene36953:CDS |
| TTCATCACCTGACAAGTTAA+TGG | 0.282278 | 8.4:-76970944 | MS.gene36953:CDS |
| GTTGGCCCTTGTAGCTTAAC+TGG | 0.282373 | 8.4:-76970293 | MS.gene36953:CDS |
| CTCCATCAACAGCTTATTCA+TGG | 0.283034 | 8.4:+76973418 | None:intergenic |
| CTTCGAATTGAACTTGGTTT+AGG | 0.284511 | 8.4:+76974271 | None:intergenic |
| CGTATCATCCCTCTTGAATC+TGG | 0.289816 | 8.4:-76969503 | MS.gene36953:CDS |
| ATTGGATCTGCAACAGTTAT+AGG | 0.295090 | 8.4:-76969081 | MS.gene36953:intron |
| TGTAGACCTGTTGAATAATC+TGG | 0.306622 | 8.4:+76967811 | None:intergenic |
| CTGCAGGACTTGCATCCCTT+GGG | 0.308926 | 8.4:+76972785 | None:intergenic |
| AATAAGCACTTAATGGATAA+TGG | 0.320902 | 8.4:-76974355 | MS.gene36953:CDS |
| GGATGGTAATAAGCACTTAA+TGG | 0.322988 | 8.4:-76974362 | MS.gene36953:CDS |
| AACCAAGGCAAATCAAAGTT+TGG | 0.324378 | 8.4:-76974207 | MS.gene36953:CDS |
| TGACTCACAACTCGTGTCTT+TGG | 0.332611 | 8.4:+76972849 | None:intergenic |
| TGAGCAAGGGTTTGTGCCTC+AGG | 0.333658 | 8.4:+76969103 | None:intergenic |
| TCGAAGACAACAACTTATTA+GGG | 0.339115 | 8.4:-76974254 | MS.gene36953:CDS |
| AGTGATAATGCTGGAAATTC+TGG | 0.341985 | 8.4:-76969310 | MS.gene36953:CDS |
| ATACCTTCATTCCAGGCATT+TGG | 0.342067 | 8.4:+76972575 | None:intergenic |
| CTCCTTCACAAGGTCTTATC+AGG | 0.342292 | 8.4:+76971621 | None:intergenic |
| CCTGCAGGACTTGCATCCCT+TGG | 0.343323 | 8.4:+76972784 | None:intergenic |
| TATAGTGGACGCAGTACTTC+TGG | 0.345373 | 8.4:+76971459 | None:intergenic |
| TCTCTGGCCATCATTTCTTC+AGG | 0.345556 | 8.4:+76970079 | None:intergenic |
| GCCCTTGACATTGGCCCTAC+TGG | 0.345949 | 8.4:-76969346 | MS.gene36953:CDS |
| TACAGGCTTTACGTCATCTA+AGG | 0.346410 | 8.4:-76971227 | MS.gene36953:intron |
| TCTTGAGATACCTTCATTCC+AGG | 0.353242 | 8.4:+76972568 | None:intergenic |
| AAGTTTGGTTTCAGAATAGA+AGG | 0.353326 | 8.4:-76974192 | MS.gene36953:intron |
| CAAGGCATCAATGCTATTAC+AGG | 0.357953 | 8.4:-76970863 | MS.gene36953:intron |
| CTGTGTAAGCATCCATATTA+TGG | 0.372634 | 8.4:+76970330 | None:intergenic |
| TTTGCTTTATGTTTGTGAAC+TGG | 0.383504 | 8.4:-76967492 | MS.gene36953:CDS |
| CTGAATCTTTCCTTTGGCAA+TGG | 0.387020 | 8.4:-76970919 | MS.gene36953:CDS |
| CATCCACATTGTTGATCATA+TGG | 0.387385 | 8.4:-76971588 | MS.gene36953:CDS |
| GGATCTCGCGTAGGAAATTA+CGG | 0.391373 | 8.4:-76970272 | MS.gene36953:CDS |
| TCAGGTTGCAAGGTGTGAAT+AGG | 0.391944 | 8.4:-76973451 | MS.gene36953:CDS |
| AGAATGGGCAGACCATAATA+TGG | 0.399984 | 8.4:-76970342 | MS.gene36953:CDS |
| ATGGGTCTGAATCTTTCCTT+TGG | 0.411939 | 8.4:-76970925 | MS.gene36953:CDS |
| TCCTAAAAGATCGTCCTTTG+TGG | 0.414014 | 8.4:-76972144 | MS.gene36953:CDS |
| CCATCATTTCTTCAGGAGAA+TGG | 0.415643 | 8.4:+76970086 | None:intergenic |
| GCATGTGAACTCAGATTAGA+AGG | 0.416254 | 8.4:+76969151 | None:intergenic |
| CTAAGTGCCCGCAGAGCTGC+TGG | 0.418507 | 8.4:+76971153 | None:intergenic |
| GAATTTCTTTCAAAGGCTAC+TGG | 0.419741 | 8.4:-76972616 | MS.gene36953:CDS |
| AACCAAACTTTGATTTGCCT+TGG | 0.419790 | 8.4:+76974205 | None:intergenic |
| ATACTAACCGGCTCAATCTC+TGG | 0.425078 | 8.4:+76971130 | None:intergenic |
| ATATAATACTGCGAACATAT+TGG | 0.427160 | 8.4:+76969206 | None:intergenic |
| CATTGTTGATCATATGGACT+TGG | 0.428519 | 8.4:-76971582 | MS.gene36953:CDS |
| GTTTCTCAGCCCAATGTCAC+TGG | 0.430041 | 8.4:-76971189 | MS.gene36953:CDS |
| TTCAGACCCATTAACTTGTC+AGG | 0.430051 | 8.4:+76970937 | None:intergenic |
| TCAAGAGCTTCAACCTGCTC+AGG | 0.430316 | 8.4:+76974313 | None:intergenic |
| CCAAGGGATGCAAGTCCTGC+AGG | 0.431428 | 8.4:-76972784 | MS.gene36953:CDS |
| AGGCACAAACCCTTGCTCAT+TGG | 0.433054 | 8.4:-76969099 | MS.gene36953:CDS |
| CTCAATGGATTTACTGATGA+AGG | 0.445576 | 8.4:-76971018 | MS.gene36953:CDS |
| TCATCACCTGACAAGTTAAT+GGG | 0.446259 | 8.4:-76970943 | MS.gene36953:CDS |
| CATCATTTCTTCAGGAGAAT+GGG | 0.448210 | 8.4:+76970087 | None:intergenic |
| TATGTTCACGCAGGAACCTT+AGG | 0.448822 | 8.4:+76970369 | None:intergenic |
| AGCAAGAGCTTGTGGTCTAG+TGG | 0.450671 | 8.4:-76972277 | MS.gene36953:CDS |
| TGTGGCACAGGAGGCATGCT+TGG | 0.453662 | 8.4:+76971677 | None:intergenic |
| TCAGCAAATGAAGCATCAAT+TGG | 0.455580 | 8.4:+76969554 | None:intergenic |
| TTCACTTTCTCAAACCAGGC+AGG | 0.455998 | 8.4:-76967933 | MS.gene36953:CDS |
| TGCTAAAGTTTCTTCTGCAA+TGG | 0.458146 | 8.4:+76972639 | None:intergenic |
| CTTCCCCACCCAGTGACATT+GGG | 0.458697 | 8.4:+76971180 | None:intergenic |
| AAGACGACCAGCAGCTCTGC+GGG | 0.458787 | 8.4:-76971160 | MS.gene36953:CDS |
| CCAGCATGTCAAGCCCTGCC+TGG | 0.459209 | 8.4:+76967919 | None:intergenic |
| TATTGAGCTGCTTTATATGC+AGG | 0.461758 | 8.4:-76972062 | MS.gene36953:intron |
| CTTGCATCTGCCCTTGACAT+TGG | 0.463238 | 8.4:-76969355 | MS.gene36953:CDS |
| GAGAAAATATTTGATGATAA+TGG | 0.466405 | 8.4:-76967861 | MS.gene36953:CDS |
| CGAAATCCAGAGGGTAGAAG+TGG | 0.468647 | 8.4:+76969524 | None:intergenic |
| CCAGGCAGGGCTTGACATGC+TGG | 0.469882 | 8.4:-76967919 | MS.gene36953:CDS |
| TCTTCCCCACCCAGTGACAT+TGG | 0.470531 | 8.4:+76971179 | None:intergenic |
| CGAGAGAGCTGTTGCTTGGA+AGG | 0.471268 | 8.4:-76967545 | MS.gene36953:CDS |
| TGCATTACAAGATATAAGTC+TGG | 0.474357 | 8.4:-76967883 | MS.gene36953:CDS |
| GTCTTCTGAGCCAGCACCAT+CGG | 0.476703 | 8.4:+76971429 | None:intergenic |
| TTTAGCAGAATTTCTTTCAA+AGG | 0.476915 | 8.4:-76972623 | MS.gene36953:CDS |
| GCTGGCTCAGAAGACTACTA+TGG | 0.477145 | 8.4:-76971421 | MS.gene36953:CDS |
| CAGCCCAATGTCACTGGGTG+GGG | 0.478064 | 8.4:-76971183 | MS.gene36953:CDS |
| GGTTTCAATGAGGCACTCAA+TGG | 0.478467 | 8.4:-76971033 | MS.gene36953:CDS |
| TGTATTATGTTGCACTCTAA+AGG | 0.481143 | 8.4:-76968324 | MS.gene36953:intron |
| GCAAGATCAAGAGTGCGATT+AGG | 0.482451 | 8.4:+76969373 | None:intergenic |
| TGCAGGACTTGCATCCCTTG+GGG | 0.482836 | 8.4:+76972786 | None:intergenic |
| GTTGAGTGGGTCCAAATGCC+TGG | 0.484697 | 8.4:-76972586 | MS.gene36953:CDS |
| CTGATATGGACAGCCTGGTC+CGG | 0.485461 | 8.4:-76972346 | MS.gene36953:intron |
| GTAGACCTGTTGAATAATCT+GGG | 0.486629 | 8.4:+76967812 | None:intergenic |
| TGTTGTCTTCGAATTGAACT+TGG | 0.487679 | 8.4:+76974265 | None:intergenic |
| CAGCATTATCACTTGATGCT+CGG | 0.488270 | 8.4:+76969320 | None:intergenic |
| ACAGCTGCTGCCATTAAAGT+TGG | 0.491933 | 8.4:-76970311 | MS.gene36953:CDS |
| AAATGATAGGTTGCAGAAGC+AGG | 0.492355 | 8.4:-76973393 | MS.gene36953:CDS |
| ATTCCAATGGAATCCGGACC+AGG | 0.494411 | 8.4:+76972333 | None:intergenic |
| GCTATTTCTCATGGTTGCAC+TGG | 0.496228 | 8.4:-76972306 | MS.gene36953:CDS |
| GGTTCAATATTAGAAAGAAT+AGG | 0.496315 | 8.4:+76974226 | None:intergenic |
| TTGCAACAGAAACAATTCTC+TGG | 0.496348 | 8.4:+76970063 | None:intergenic |
| TCTAATCTGAGTTCACATGC+TGG | 0.496973 | 8.4:-76969148 | MS.gene36953:CDS |
| AAAGTGCTAACGCTACCCTT+TGG | 0.500197 | 8.4:+76969176 | None:intergenic |
| CATACGAGAGAGCTGTTGCT+TGG | 0.500411 | 8.4:-76967549 | MS.gene36953:CDS |
| TACTGGAACCGCTGTTGAGT+GGG | 0.504776 | 8.4:-76972599 | MS.gene36953:CDS |
| TTGATGCTCGGTTTCCAGTA+GGG | 0.507932 | 8.4:+76969332 | None:intergenic |
| ATATGAATCACCGATGGTGC+TGG | 0.508312 | 8.4:-76971439 | MS.gene36953:CDS |
| AGCCATGAATAAGCTGTTGA+TGG | 0.508413 | 8.4:-76973420 | MS.gene36953:CDS |
| TACACTTCTGTTGTAGAAGA+TGG | 0.508557 | 8.4:-76971895 | MS.gene36953:CDS |
| ATCAATCACACTCACCCTGC+AGG | 0.508922 | 8.4:+76972769 | None:intergenic |
| CGAGATCCAGTTAAGCTACA+AGG | 0.509433 | 8.4:+76970287 | None:intergenic |
| GGAATCGTCGCTATTTCTCA+TGG | 0.511365 | 8.4:-76972315 | MS.gene36953:CDS |
| ATCAAGAGTGCGATTAGGAC+TGG | 0.512797 | 8.4:+76969378 | None:intergenic |
| CTAATCTGAGTTCACATGCT+GGG | 0.513411 | 8.4:-76969147 | MS.gene36953:CDS |
| CCATTCTCCTGAAGAAATGA+TGG | 0.515783 | 8.4:-76970086 | MS.gene36953:CDS |
| GGTCCAAATGCCTGGAATGA+AGG | 0.515906 | 8.4:-76972578 | MS.gene36953:intron |
| ACCATCGGTGATTCATATAG+TGG | 0.517896 | 8.4:+76971444 | None:intergenic |
| GCGTGAACATAGATCAGAAT+GGG | 0.519247 | 8.4:-76970357 | MS.gene36953:CDS |
| CTTGATGCTCGGTTTCCAGT+AGG | 0.520139 | 8.4:+76969331 | None:intergenic |
| TCTCACCTCGTGTATGAGAA+TGG | 0.523269 | 8.4:-76973368 | MS.gene36953:CDS |
| TTCCAGTAGGGCCAATGTCA+AGG | 0.524338 | 8.4:+76969344 | None:intergenic |
| TGTTGATCATATGGACTTGG+AGG | 0.525009 | 8.4:-76971579 | MS.gene36953:intron |
| CAGCCTGGTCCGGATTCCAT+TGG | 0.526878 | 8.4:-76972336 | MS.gene36953:CDS |
| ATGATCAACAATGTGGATGA+TGG | 0.527724 | 8.4:+76971592 | None:intergenic |
| GATCTCGCGTAGGAAATTAC+GGG | 0.527880 | 8.4:-76970271 | MS.gene36953:CDS |
| TGTTGATGGAGGAAAATGAT+AGG | 0.529771 | 8.4:-76973406 | MS.gene36953:CDS |
| TGCCTCCAGCCATTCTCCTA+AGG | 0.530016 | 8.4:-76970385 | MS.gene36953:CDS |
| GCGACGATTCCAATGGAATC+CGG | 0.530819 | 8.4:+76972327 | None:intergenic |
| TTGGTAGCTCTGCAGTGGAA+TGG | 0.533406 | 8.4:-76969618 | MS.gene36953:intron |
| GCTCAATAGTGTGTGCTAGC+GGG | 0.534034 | 8.4:+76970234 | None:intergenic |
| GGAATGGATGAGAATGCTAT+TGG | 0.534330 | 8.4:-76969602 | MS.gene36953:CDS |
| GATGCCCCACTTCTACCCTC+TGG | 0.535941 | 8.4:-76969530 | MS.gene36953:CDS |
| AGCAGGTTGAAGCTCTTGAA+AGG | 0.536769 | 8.4:-76974309 | MS.gene36953:CDS |
| TTGGAAGTTATTAAGTTGGA+CGG | 0.536877 | 8.4:-76970115 | MS.gene36953:CDS |
| CGAGCATCAAGTGATAATGC+TGG | 0.543560 | 8.4:-76969319 | MS.gene36953:CDS |
| GCTCTAACAAAATGTGGCAC+AGG | 0.544412 | 8.4:+76971665 | None:intergenic |
| CGAGCAGGTGCCAATGTGGT+TGG | 0.545131 | 8.4:+76971937 | None:intergenic |
| TGAGTTCACATGCTGGGCTG+AGG | 0.546668 | 8.4:-76969141 | MS.gene36953:CDS |
| TCAGCCCAATGTCACTGGGT+GGG | 0.546882 | 8.4:-76971184 | MS.gene36953:CDS |
| CTGTAATAGCATTGATGCCT+TGG | 0.547972 | 8.4:+76970864 | None:intergenic |
| AGCAAATATAAGTTCTGCAC+AGG | 0.548261 | 8.4:+76969577 | None:intergenic |
| GCAGGACTTGCATCCCTTGG+GGG | 0.548645 | 8.4:+76972787 | None:intergenic |
| GCTACTATGGCATGCAAGGA+TGG | 0.548904 | 8.4:-76974379 | None:intergenic |
| ACAGCTCTCTCGTATGAGAC+AGG | 0.550158 | 8.4:+76967556 | None:intergenic |
| TGGTATTTGTCTCTCAAGCA+TGG | 0.550328 | 8.4:-76967584 | MS.gene36953:CDS |
| GAGCAAGGGTTTGTGCCTCA+GGG | 0.553358 | 8.4:+76969104 | None:intergenic |
| ACGCAGGAACCTTAGGAGAA+TGG | 0.554205 | 8.4:+76970376 | None:intergenic |
| AACAACCAGAAGTCGCGAGC+AGG | 0.555588 | 8.4:+76971922 | None:intergenic |
| TATTATATCATCAGTCCAAA+GGG | 0.557286 | 8.4:-76969191 | MS.gene36953:CDS |
| CTCAGCCCAATGTCACTGGG+TGG | 0.560247 | 8.4:-76971185 | MS.gene36953:CDS |
| AGGAACCTTAGGAGAATGGC+TGG | 0.561926 | 8.4:+76970380 | None:intergenic |
| AAAGAGTCTTTCAGGTTGCA+AGG | 0.562032 | 8.4:-76973461 | MS.gene36953:CDS |
| AGTACTTCTGGAACGCTCCA+TGG | 0.562310 | 8.4:+76971471 | None:intergenic |
| CTACTTAAATCTAATACTGA+AGG | 0.567758 | 8.4:-76968389 | MS.gene36953:CDS |
| CTGATAAGACCTTGTGAAGG+AGG | 0.568786 | 8.4:-76971620 | MS.gene36953:CDS |
| TGCTCAATAGTGTGTGCTAG+CGG | 0.571029 | 8.4:+76970233 | None:intergenic |
| GCAAGAGAATGTAGCATGCA+TGG | 0.572833 | 8.4:-76969233 | MS.gene36953:CDS |
| TACAGCTACTATGGCATGCA+AGG | 0.573092 | 8.4:-76974383 | None:intergenic |
| TTCTTTCTAATATTGAACCA+AGG | 0.579624 | 8.4:-76974222 | MS.gene36953:CDS |
| GCAAGAGCTTGTGGTCTAGT+GGG | 0.580275 | 8.4:-76972276 | MS.gene36953:CDS |
| GAGCAGACATGCTGCCTAGT+GGG | 0.581394 | 8.4:-76971646 | MS.gene36953:CDS |
| TCACTTTCTCAAACCAGGCA+GGG | 0.582033 | 8.4:-76967932 | MS.gene36953:CDS |
| CTACTGGAACCGCTGTTGAG+TGG | 0.586215 | 8.4:-76972600 | MS.gene36953:CDS |
| TAGTGGGACTAGAACCCACT+AGG | 0.586368 | 8.4:-76972260 | MS.gene36953:CDS |
| TGATGAAGGATGGACAATGA+TGG | 0.587248 | 8.4:-76971004 | MS.gene36953:CDS |
| TTTCTCAGCCCAATGTCACT+GGG | 0.588485 | 8.4:-76971188 | MS.gene36953:CDS |
| TCCAGTAGGGCCAATGTCAA+GGG | 0.589155 | 8.4:+76969345 | None:intergenic |
| GTATTATATCATCAGTCCAA+AGG | 0.589604 | 8.4:-76969192 | MS.gene36953:CDS |
| CACTTAGCCAGAGATTGAGC+CGG | 0.590279 | 8.4:-76971137 | MS.gene36953:intron |
| TTGTAGATGCTACTTAGGCG+TGG | 0.590476 | 8.4:-76968414 | MS.gene36953:intron |
| AGTGTTCACTTTCTCAAACC+AGG | 0.592651 | 8.4:-76967937 | MS.gene36953:CDS |
| ATGGATTTACTGATGAAGGA+TGG | 0.595578 | 8.4:-76971014 | MS.gene36953:CDS |
| TGTACGTTATACACCTGAGC+AGG | 0.597312 | 8.4:-76974326 | MS.gene36953:CDS |
| TGTTGCAGATCCAATGAGCA+AGG | 0.597888 | 8.4:+76969089 | None:intergenic |
| GCTTTATGCACCAACCACAT+TGG | 0.599013 | 8.4:-76971947 | MS.gene36953:CDS |
| AGAAATAGCGACGATTCCAA+TGG | 0.599267 | 8.4:+76972320 | None:intergenic |
| GGTATTTGTCTCTCAAGCAT+GGG | 0.600880 | 8.4:-76967583 | MS.gene36953:CDS |
| TGTTTGCAGGGGTTTCAATG+AGG | 0.601250 | 8.4:-76971043 | MS.gene36953:intron |
| ATAAGACCTTGTGAAGGAGG+TGG | 0.601925 | 8.4:-76971617 | MS.gene36953:CDS |
| AATAGTTCCACCGCTAGCAG+TGG | 0.607297 | 8.4:+76972081 | None:intergenic |
| TCTGGACCACTTACCCTAGT+GGG | 0.608488 | 8.4:+76972246 | None:intergenic |
| CAAGGGATGCAAGTCCTGCA+GGG | 0.611498 | 8.4:-76972783 | MS.gene36953:intron |
| AACACAGCATTACTGACAGA+AGG | 0.611683 | 8.4:+76970892 | None:intergenic |
| CTAACAAAATGTGGCACAGG+AGG | 0.612984 | 8.4:+76971668 | None:intergenic |
| AATGTGCTGCCCACTGCTAG+CGG | 0.616645 | 8.4:-76972091 | MS.gene36953:CDS |
| ATGTCTGCTCTAACAAAATG+TGG | 0.616648 | 8.4:+76971659 | None:intergenic |
| GGTGTCGCAGCAAGAGCTTG+TGG | 0.617510 | 8.4:-76972285 | MS.gene36953:CDS |
| GATGAAGGATGGACAATGAT+GGG | 0.617941 | 8.4:-76971003 | MS.gene36953:CDS |
| ATAGTTCCACCGCTAGCAGT+GGG | 0.625406 | 8.4:+76972082 | None:intergenic |
| AGCAAGGGTTTGTGCCTCAG+GGG | 0.625642 | 8.4:+76969105 | None:intergenic |
| GAAATCCAGAGGGTAGAAGT+GGG | 0.626534 | 8.4:+76969525 | None:intergenic |
| CATGAATAAGCTGTTGATGG+AGG | 0.627357 | 8.4:-76973417 | MS.gene36953:CDS |
| AGAAGGAAATCCATTGCCAA+AGG | 0.629899 | 8.4:+76970909 | None:intergenic |
| GGTCTTATCAGGTACCCACT+AGG | 0.630106 | 8.4:+76971632 | None:intergenic |
| GTGCTGCCCACTGCTAGCGG+TGG | 0.632331 | 8.4:-76972088 | MS.gene36953:CDS |
| TGCGTGAACATAGATCAGAA+TGG | 0.632509 | 8.4:-76970358 | MS.gene36953:CDS |
| AGCATCAGAGTGGTGCCACA+AGG | 0.633858 | 8.4:+76968346 | None:intergenic |
| GTTGCAGATCCAATGAGCAA+GGG | 0.634819 | 8.4:+76969090 | None:intergenic |
| AGACACGAGTTGTGAGTCAG+TGG | 0.635890 | 8.4:-76972845 | MS.gene36953:CDS |
| AGCACACACTATTGAGCATG+AGG | 0.637565 | 8.4:-76970228 | MS.gene36953:CDS |
| ATCTGGACCACTTACCCTAG+TGG | 0.637854 | 8.4:+76972245 | None:intergenic |
| GCTGGGCTGAGGTCACCGCT+AGG | 0.640780 | 8.4:-76969130 | MS.gene36953:CDS |
| AGAGCAGACATGCTGCCTAG+TGG | 0.643425 | 8.4:-76971647 | MS.gene36953:CDS |
| TGGACAATGATGGGCAATGA+CGG | 0.644598 | 8.4:-76970994 | MS.gene36953:CDS |
| GAAGACGACCAGCAGCTCTG+CGG | 0.647462 | 8.4:-76971161 | MS.gene36953:CDS |
| CATTGACAATATCAACAGCT+CGG | 0.648913 | 8.4:+76972110 | None:intergenic |
| GTCGCGAGCAGGTGCCAATG+TGG | 0.649892 | 8.4:+76971933 | None:intergenic |
| TAATGCTGTGTTATGTGCCA+AGG | 0.650005 | 8.4:-76970881 | MS.gene36953:CDS |
| TAGACCTGTTGAATAATCTG+GGG | 0.651755 | 8.4:+76967813 | None:intergenic |
| ATGGAACCACCTCCTTCACA+AGG | 0.652482 | 8.4:+76971611 | None:intergenic |
| GAGATCCAGTTAAGCTACAA+GGG | 0.653186 | 8.4:+76970288 | None:intergenic |
| AATCTGTACTCAAGTCCTTG+TGG | 0.653586 | 8.4:-76968361 | MS.gene36953:CDS |
| AGCTTAACTGGATCTCGCGT+AGG | 0.653798 | 8.4:-76970281 | MS.gene36953:CDS |
| TCCACTATATGAATCACCGA+TGG | 0.655585 | 8.4:-76971445 | MS.gene36953:CDS |
| TGTGAGTCAGTGGTGACTAG+TGG | 0.658501 | 8.4:-76972835 | MS.gene36953:CDS |
| CTGAACATAATGTCAAACAA+AGG | 0.670183 | 8.4:+76967468 | None:intergenic |
| AGGGATGATACGAAATCCAG+AGG | 0.676709 | 8.4:+76969514 | None:intergenic |
| GTGTGCCTTAGATCTGTGAG+AGG | 0.677270 | 8.4:-76971724 | MS.gene36953:intron |
| ATCTCGCGTAGGAAATTACG+GGG | 0.678972 | 8.4:-76970270 | MS.gene36953:CDS |
| GGCTCAGAAGACTACTATGG+CGG | 0.682781 | 8.4:-76971418 | MS.gene36953:intron |
| TACCTGATAAGACCTTGTGA+AGG | 0.684274 | 8.4:-76971623 | MS.gene36953:CDS |
| TGTAGAAGATGGCAGCCTAG+TGG | 0.684422 | 8.4:-76971884 | MS.gene36953:intron |
| GGGATGATACGAAATCCAGA+GGG | 0.685666 | 8.4:+76969515 | None:intergenic |
| AGTCCATATGATCAACAATG+TGG | 0.687782 | 8.4:+76971585 | None:intergenic |
| CGGCAATCACGAAACCACAA+AGG | 0.688045 | 8.4:+76972130 | None:intergenic |
| TGTGCCTCAGGGGTTCCTAG+CGG | 0.689894 | 8.4:+76969115 | None:intergenic |
| AAATCCAGAGGGTAGAAGTG+GGG | 0.695513 | 8.4:+76969526 | None:intergenic |
| AGTGGGACTAGAACCCACTA+GGG | 0.698187 | 8.4:-76972259 | MS.gene36953:intron |
| ATTCTGATCTATGTTCACGC+AGG | 0.699759 | 8.4:+76970360 | None:intergenic |
| ACACACTATTGAGCATGAGG+AGG | 0.703064 | 8.4:-76970225 | MS.gene36953:intron |
| AGACCTGTTGAATAATCTGG+GGG | 0.704064 | 8.4:+76967814 | None:intergenic |
| AACATAATACAGCATCAGAG+TGG | 0.704741 | 8.4:+76968336 | None:intergenic |
| CATTTGGACCCACTCAACAG+CGG | 0.709948 | 8.4:+76972591 | None:intergenic |
| TGGTTTGAGAAAGTGAACAC+TGG | 0.711249 | 8.4:+76967939 | None:intergenic |
| AACCTTAGGAGAATGGCTGG+AGG | 0.716615 | 8.4:+76970383 | None:intergenic |
| AATAGCCATTCTCATACACG+AGG | 0.748291 | 8.4:+76973363 | None:intergenic |
| GTCACCGCTAGGAACCCCTG+AGG | 0.761147 | 8.4:-76969119 | MS.gene36953:CDS |
| TCTCGCGTAGGAAATTACGG+GGG | 0.824268 | 8.4:-76970269 | MS.gene36953:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 76967480 | 76974395 | 76967480 | ID=MS.gene36953 |
| chr8.4 | mRNA | 76967480 | 76974395 | 76967480 | ID=MS.gene36953.t1;Parent=MS.gene36953 |
| chr8.4 | exon | 76974193 | 76974395 | 76974193 | ID=MS.gene36953.t1.exon1;Parent=MS.gene36953.t1 |
| chr8.4 | CDS | 76974193 | 76974395 | 76974193 | ID=cds.MS.gene36953.t1;Parent=MS.gene36953.t1 |
| chr8.4 | exon | 76973343 | 76973502 | 76973343 | ID=MS.gene36953.t1.exon2;Parent=MS.gene36953.t1 |
| chr8.4 | CDS | 76973343 | 76973502 | 76973343 | ID=cds.MS.gene36953.t1;Parent=MS.gene36953.t1 |
| chr8.4 | exon | 76972784 | 76972875 | 76972784 | ID=MS.gene36953.t1.exon3;Parent=MS.gene36953.t1 |
| chr8.4 | CDS | 76972784 | 76972875 | 76972784 | ID=cds.MS.gene36953.t1;Parent=MS.gene36953.t1 |
| chr8.4 | exon | 76972579 | 76972669 | 76972579 | ID=MS.gene36953.t1.exon4;Parent=MS.gene36953.t1 |
| chr8.4 | CDS | 76972579 | 76972669 | 76972579 | ID=cds.MS.gene36953.t1;Parent=MS.gene36953.t1 |
| chr8.4 | exon | 76972260 | 76972355 | 76972260 | ID=MS.gene36953.t1.exon5;Parent=MS.gene36953.t1 |
| chr8.4 | CDS | 76972260 | 76972355 | 76972260 | ID=cds.MS.gene36953.t1;Parent=MS.gene36953.t1 |
| chr8.4 | exon | 76972063 | 76972176 | 76972063 | ID=MS.gene36953.t1.exon6;Parent=MS.gene36953.t1 |
| chr8.4 | CDS | 76972063 | 76972176 | 76972063 | ID=cds.MS.gene36953.t1;Parent=MS.gene36953.t1 |
| chr8.4 | exon | 76971885 | 76971968 | 76971885 | ID=MS.gene36953.t1.exon7;Parent=MS.gene36953.t1 |
| chr8.4 | CDS | 76971885 | 76971968 | 76971885 | ID=cds.MS.gene36953.t1;Parent=MS.gene36953.t1 |
| chr8.4 | exon | 76971580 | 76971735 | 76971580 | ID=MS.gene36953.t1.exon8;Parent=MS.gene36953.t1 |
| chr8.4 | CDS | 76971580 | 76971735 | 76971580 | ID=cds.MS.gene36953.t1;Parent=MS.gene36953.t1 |
| chr8.4 | exon | 76971419 | 76971493 | 76971419 | ID=MS.gene36953.t1.exon9;Parent=MS.gene36953.t1 |
| chr8.4 | CDS | 76971419 | 76971493 | 76971419 | ID=cds.MS.gene36953.t1;Parent=MS.gene36953.t1 |
| chr8.4 | exon | 76971138 | 76971244 | 76971138 | ID=MS.gene36953.t1.exon10;Parent=MS.gene36953.t1 |
| chr8.4 | CDS | 76971138 | 76971244 | 76971138 | ID=cds.MS.gene36953.t1;Parent=MS.gene36953.t1 |
| chr8.4 | exon | 76970864 | 76971056 | 76970864 | ID=MS.gene36953.t1.exon11;Parent=MS.gene36953.t1 |
| chr8.4 | CDS | 76970864 | 76971056 | 76970864 | ID=cds.MS.gene36953.t1;Parent=MS.gene36953.t1 |
| chr8.4 | exon | 76970226 | 76970411 | 76970226 | ID=MS.gene36953.t1.exon12;Parent=MS.gene36953.t1 |
| chr8.4 | CDS | 76970226 | 76970411 | 76970226 | ID=cds.MS.gene36953.t1;Parent=MS.gene36953.t1 |
| chr8.4 | exon | 76970063 | 76970140 | 76970063 | ID=MS.gene36953.t1.exon13;Parent=MS.gene36953.t1 |
| chr8.4 | CDS | 76970063 | 76970140 | 76970063 | ID=cds.MS.gene36953.t1;Parent=MS.gene36953.t1 |
| chr8.4 | exon | 76969499 | 76969633 | 76969499 | ID=MS.gene36953.t1.exon14;Parent=MS.gene36953.t1 |
| chr8.4 | CDS | 76969499 | 76969633 | 76969499 | ID=cds.MS.gene36953.t1;Parent=MS.gene36953.t1 |
| chr8.4 | exon | 76969082 | 76969407 | 76969082 | ID=MS.gene36953.t1.exon15;Parent=MS.gene36953.t1 |
| chr8.4 | CDS | 76969082 | 76969407 | 76969082 | ID=cds.MS.gene36953.t1;Parent=MS.gene36953.t1 |
| chr8.4 | exon | 76968325 | 76968430 | 76968325 | ID=MS.gene36953.t1.exon16;Parent=MS.gene36953.t1 |
| chr8.4 | CDS | 76968325 | 76968430 | 76968325 | ID=cds.MS.gene36953.t1;Parent=MS.gene36953.t1 |
| chr8.4 | exon | 76967818 | 76967967 | 76967818 | ID=MS.gene36953.t1.exon17;Parent=MS.gene36953.t1 |
| chr8.4 | CDS | 76967818 | 76967967 | 76967818 | ID=cds.MS.gene36953.t1;Parent=MS.gene36953.t1 |
| chr8.4 | exon | 76967480 | 76967626 | 76967480 | ID=MS.gene36953.t1.exon18;Parent=MS.gene36953.t1 |
| chr8.4 | CDS | 76967480 | 76967626 | 76967480 | ID=cds.MS.gene36953.t1;Parent=MS.gene36953.t1 |
| Gene Sequence |
| Protein sequence |
