AlfalfaGEDB | MS.gene37264

M. sativa cultivar XinJiangDaYe / MS.gene37264

KEGG: -
GO: GO:0000723 GO:0003674 GO:0003678 GO:0003824 GO:0004386 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008094 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032200 GO:0033554 GO:0034641 GO:0042592 GO:0042623 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0060249 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0090304 GO:0140097 GO:1901360
PFAM: PF05970.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene37264.t1	XP_024638646.1	81.7	109	20	0	7	115	1335	1443	6.60E-45	189.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene37264.t1	A0A072V9G8	76.1	113	23	1	7	115	225	337	2.7e-40	174.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene054174	MS.gene37264	0.814441	1.54E-51	-1.69E-46
MS.gene054175	MS.gene37264	0.8182	2.22E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene37264.t1	MTR_2g055890	76.106	113	23	1	7	115	225	337	4.95e-54	174
MS.gene37264.t1	MTR_4g037500	75.229	109	27	0	7	115	157	265	8.40e-54	171
MS.gene37264.t1	MTR_0014s0220	75.472	106	22	1	7	108	318	423	4.82e-48	164
MS.gene37264.t1	MTR_7g050355	53.913	115	47	1	7	115	337	451	8.76e-36	128
MS.gene37264.t1	MTR_1g054110	50.435	115	51	1	7	115	135	249	1.35e-32	115
MS.gene37264.t1	MTR_4g062370	49.565	115	52	1	7	115	385	499	3.21e-32	119
MS.gene37264.t1	MTR_2g016390	52.174	115	49	1	7	115	175	289	4.08e-32	118
MS.gene37264.t1	MTR_5g032840	57.732	97	41	0	19	115	104	200	4.29e-32	116
MS.gene37264.t1	MTR_0296s0010	49.107	112	48	2	7	112	12	120	2.78e-31	113
MS.gene37264.t1	MTR_4g076760	50.000	120	49	2	7	115	196	315	3.03e-31	115
MS.gene37264.t1	MTR_7g060105	52.577	97	46	0	19	115	55	151	4.14e-31	108
MS.gene37264.t1	MTR_5g053570	75.000	68	13	1	7	70	397	464	1.55e-28	107
MS.gene37264.t1	MTR_6g071545	46.957	115	55	2	7	115	72	186	4.12e-28	104
MS.gene37264.t1	MTR_1g057360	46.296	108	52	1	7	108	108	215	1.10e-27	103
MS.gene37264.t1	MTR_5g071700	51.064	94	40	1	7	94	95	188	2.23e-27	103
MS.gene37264.t1	MTR_1g044190	46.491	114	55	1	8	115	274	387	2.31e-27	104
MS.gene37264.t1	MTR_1g012040	43.333	120	62	1	2	115	147	266	1.10e-26	103
MS.gene37264.t1	MTR_0017s0290	42.609	115	60	1	7	115	151	265	6.68e-26	100
MS.gene37264.t1	MTR_1g021210	45.217	115	57	1	7	115	351	465	1.70e-25	99.8
MS.gene37264.t1	MTR_6g055280	74.138	58	10	1	7	59	65	122	2.50e-25	93.6
MS.gene37264.t1	MTR_5g061500	43.478	115	59	1	7	115	151	265	5.60e-25	98.2
MS.gene37264.t1	MTR_2g066670	43.478	115	63	1	1	115	98	210	2.15e-24	96.3
MS.gene37264.t1	MTR_2g064230	43.478	115	63	1	1	115	92	204	2.22e-24	96.7
MS.gene37264.t1	MTR_0550s0010	44.762	105	58	0	11	115	5	109	3.99e-24	93.2
MS.gene37264.t1	MTR_8g469120	40.708	113	61	1	9	115	1	113	6.27e-23	87.4
MS.gene37264.t1	MTR_0003s0390	41.739	115	61	1	7	115	190	304	6.78e-23	91.7
MS.gene37264.t1	MTR_8g023250	40.708	113	61	1	9	115	1	113	1.04e-21	87.4
MS.gene37264.t1	MTR_3g034020	43.119	109	60	1	7	115	149	255	1.40e-21	88.2
MS.gene37264.t1	MTR_3g052540	38.938	113	63	1	9	115	1	113	2.01e-21	86.3
MS.gene37264.t1	MTR_2g084465	40.909	110	59	1	7	110	87	196	2.89e-21	86.3
MS.gene37264.t1	MTR_8g045705	41.053	95	56	0	21	115	68	162	4.26e-21	85.5
MS.gene37264.t1	MTR_1g107470	42.105	114	55	2	7	114	111	219	6.21e-21	86.3
MS.gene37264.t1	MTR_3g434910	42.202	109	52	2	7	109	193	296	2.66e-20	84.0
MS.gene37264.t1	MTR_7g027013	40.517	116	47	3	6	115	456	555	5.89e-19	81.3
MS.gene37264.t1	MTR_2g047010	59.016	61	25	0	32	92	16	76	1.92e-18	77.0
MS.gene37264.t1	MTR_4g050743	59.016	61	25	0	32	92	16	76	2.03e-18	77.0
MS.gene37264.t1	MTR_4g086180	36.792	106	56	3	7	112	153	247	3.62e-18	78.6
MS.gene37264.t1	MTR_5g054110	36.697	109	58	3	7	115	35	132	8.03e-18	74.3
MS.gene37264.t1	MTR_3g100990	43.243	74	42	0	42	115	187	260	1.79e-17	77.0
MS.gene37264.t1	MTR_8g061330	51.220	82	34	1	7	82	142	223	3.92e-17	75.9
MS.gene37264.t1	MTR_0073s0010	46.512	86	40	1	7	86	161	246	4.59e-17	73.9
MS.gene37264.t1	MTR_1g093700	34.188	117	72	1	4	115	20	136	5.23e-17	74.7
MS.gene37264.t1	MTR_8g022960	43.373	83	47	0	33	115	2	84	5.35e-17	74.3
MS.gene37264.t1	MTR_7g018160	51.724	87	29	2	11	84	3	89	1.02e-16	73.9
MS.gene37264.t1	MTR_3g113230	57.143	70	24	1	7	70	176	245	1.53e-16	72.8
MS.gene37264.t1	MTR_1g106680	52.239	67	32	0	21	87	10	76	1.68e-16	68.6
MS.gene37264.t1	MTR_4g132830	46.667	90	42	1	7	90	98	187	3.15e-16	72.8
MS.gene37264.t1	MTR_5g024850	43.750	96	48	1	1	90	110	205	6.39e-16	72.4
MS.gene37264.t1	MTR_5g071480	46.237	93	44	1	3	89	33	125	7.15e-16	71.2
MS.gene37264.t1	MTR_0002s0870	55.714	70	25	1	7	70	122	191	2.19e-15	70.5
MS.gene37264.t1	MTR_1g116150	46.591	88	41	1	1	82	77	164	3.53e-15	70.1
MS.gene37264.t1	MTR_5g059380	46.591	88	41	1	1	82	108	195	3.87e-15	70.1
MS.gene37264.t1	MTR_3g032470	48.684	76	39	0	10	85	8	83	8.85e-15	67.8
MS.gene37264.t1	MTR_3g109055	48.750	80	35	1	7	80	135	214	2.28e-14	66.6
MS.gene37264.t1	MTR_5g020370	51.667	60	23	2	7	60	157	216	6.57e-14	66.6
MS.gene37264.t1	MTR_2g013330	41.429	70	41	0	46	115	197	266	9.69e-13	63.5
MS.gene37264.t1	MTR_5g032940	36.082	97	39	1	19	115	53	126	1.26e-12	62.8
MS.gene37264.t1	MTR_2g058780	55.000	60	21	1	3	56	66	125	2.48e-12	62.0
MS.gene37264.t1	MTR_3g048710	67.500	40	12	1	19	58	101	139	1.02e-11	58.5
MS.gene37264.t1	MTR_2g049690	34.188	117	68	4	7	115	146	261	1.80e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 12 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCATAAATTCTTCTTACATC+TGG	0.298534	1.2:+40161547	MS.gene37264:CDS
ATTTCTGCATTTCCATCATT+TGG	0.302976	1.2:-40161699	None:intergenic
AAATGCAGAAATTGATATAT+CGG	0.425213	1.2:+40161710	MS.gene37264:CDS
CATAAATTCTTCTTACATCT+GGG	0.477819	1.2:+40161548	MS.gene37264:CDS
AGGCTAGTCGAGCCAAATGA+TGG	0.501488	1.2:+40161687	MS.gene37264:CDS
ATGTCTGAACGATCCGCTCT+AGG	0.529790	1.2:-40161513	None:intergenic
TCTGAATGGCTGTTGAAAGT+TGG	0.539937	1.2:+40161657	MS.gene37264:CDS
CGATCCGCTCTAGGTACGAC+AGG	0.557806	1.2:-40161504	None:intergenic
TTACATCTGGGATGAATGTG+AGG	0.579872	1.2:+40161560	MS.gene37264:CDS
TTTACCTGTCGTACCTAGAG+CGG	0.619638	1.2:+40161500	MS.gene37264:CDS
TGTTGAAAGTTGGTGAAGGA+AGG	0.633000	1.2:+40161667	MS.gene37264:CDS
TGGCTGTTGAAAGTTGGTGA+AGG	0.661679	1.2:+40161663	MS.gene37264:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATGCAGAAATTGATATAT+CGG	+	chr1.2:40161710-40161729	MS.gene37264:CDS	20.0%
!!	AATTAACAAAACTGATCATA+TGG	+	chr1.2:40161352-40161371	MS.gene37264:intron	20.0%
!!	ATTAACAAAACTGATCATAT+GGG	+	chr1.2:40161353-40161372	MS.gene37264:intron	20.0%
!!!	AGTTAAAAATGTTTTCTGAA+TGG	+	chr1.2:40161643-40161662	MS.gene37264:CDS	20.0%
!	CATAAATTCTTCTTACATCT+GGG	+	chr1.2:40161548-40161567	MS.gene37264:CDS	25.0%
	AAAGCAAAATTTATGAGCCA+TGG	-	chr1.2:40161388-40161407	None:intergenic	30.0%
	AAGCAAAATTTATGAGCCAT+GGG	-	chr1.2:40161387-40161406	None:intergenic	30.0%
	ATTTCTGCATTTCCATCATT+TGG	-	chr1.2:40161702-40161721	None:intergenic	30.0%
	GCATAAATTCTTCTTACATC+TGG	+	chr1.2:40161547-40161566	MS.gene37264:CDS	30.0%
!	ATGTCCTCTGATAAGATTTT+TGG	+	chr1.2:40161438-40161457	MS.gene37264:CDS	30.0%
!	TGTCCTCTGATAAGATTTTT+GGG	+	chr1.2:40161439-40161458	MS.gene37264:CDS	30.0%
!!!	GGAAAAGTTATTGTTTTTGG+AGG	+	chr1.2:40161462-40161481	MS.gene37264:CDS	30.0%
!	GGGGGAAAAGTTATTGTTTT+TGG	+	chr1.2:40161459-40161478	MS.gene37264:CDS	35.0%
!	GTCCTCTGATAAGATTTTTG+GGG	+	chr1.2:40161440-40161459	MS.gene37264:CDS	35.0%
!	TTGTTGCAGACGCATATTTT+TGG	-	chr1.2:40161598-40161617	None:intergenic	35.0%
!!	TCCTCTGATAAGATTTTTGG+GGG	+	chr1.2:40161441-40161460	MS.gene37264:CDS	35.0%
	TCCCCCAAAAATCTTATCAG+AGG	-	chr1.2:40161445-40161464	None:intergenic	40.0%
	TCTGAATGGCTGTTGAAAGT+TGG	+	chr1.2:40161657-40161676	MS.gene37264:CDS	40.0%
!	TTACATCTGGGATGAATGTG+AGG	+	chr1.2:40161560-40161579	MS.gene37264:CDS	40.0%
!!	TGTTGAAAGTTGGTGAAGGA+AGG	+	chr1.2:40161667-40161686	MS.gene37264:CDS	40.0%
	TTTACCTGTCGTACCTAGAG+CGG	+	chr1.2:40161500-40161519	MS.gene37264:CDS	45.0%
!!	TGGCTGTTGAAAGTTGGTGA+AGG	+	chr1.2:40161663-40161682	MS.gene37264:CDS	45.0%
	ATGTCTGAACGATCCGCTCT+AGG	-	chr1.2:40161516-40161535	None:intergenic	50.0%
!	AGGCTAGTCGAGCCAAATGA+TGG	+	chr1.2:40161687-40161706	MS.gene37264:CDS	50.0%
!	CATATGGGATGAAGCACCCA+TGG	+	chr1.2:40161368-40161387	MS.gene37264:intron	50.0%
!	CGATCCGCTCTAGGTACGAC+AGG	-	chr1.2:40161507-40161526	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	40161311	40161749	40161311	ID=MS.gene37264
chr1.2	mRNA	40161311	40161749	40161311	ID=MS.gene37264.t1;Parent=MS.gene37264
chr1.2	exon	40161311	40161326	40161311	ID=MS.gene37264.t1.exon1;Parent=MS.gene37264.t1
chr1.2	CDS	40161311	40161326	40161311	ID=cds.MS.gene37264.t1;Parent=MS.gene37264.t1
chr1.2	exon	40161418	40161749	40161418	ID=MS.gene37264.t1.exon2;Parent=MS.gene37264.t1
chr1.2	CDS	40161418	40161749	40161418	ID=cds.MS.gene37264.t1;Parent=MS.gene37264.t1

Gene Sequence

>MS.gene37264

ATGTACTATAGACAGTGTAAGTGAACTTTCTCAACTTCTAAAATTAACAAAACTGATCATATGGGATGAAGCACCCATGGCTCATAAATTTTGCTTTGATGCTTTAGATAGAACACTCAAAGACATTATGTCCTCTGATAAGATTTTTGGGGGAAAAGTTATTGTTTTTGGAGGTGACTTTAGACAGATTTTACCTGTCGTACCTAGAGCGGATCGTTCAGACATTGTGCATGCTAGCATAAATTCTTCTTACATCTGGGATGAATGTGAGGTTCTAACACTAACCAAAAATATGCGTCTGCAACAACATGCATTGTCTTCTGACTCTGAAGAGTTAAAAATGTTTTCTGAATGGCTGTTGAAAGTTGGTGAAGGAAGGCTAGTCGAGCCAAATGATGGAAATGCAGAAATTGATATATCGGCTGAGTTTTTGATATAA

Protein sequence

>MS.gene37264.t1

MYYRQYRTLKDIMSSDKIFGGKVIVFGGDFRQILPVVPRADRSDIVHASINSSYIWDECEVLTLTKNMRLQQHALSSDSEELKMFSEWLLKVGEGRLVEPNDGNAEIDISAEFLI