AlfalfaGEDB | MS.gene054175

M. sativa cultivar XinJiangDaYe / MS.gene054175

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054175.t1	XP_024634530.1	72.4	174	48	0	1	174	872	1045	1.70E-68	268.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054175.t1	A0A392MKB5	66.1	174	59	0	1	174	181	354	5.0e-62	246.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049853	MS.gene054175	0.809194	2.13E-50	-1.69E-46
MS.gene051932	MS.gene054175	0.801287	9.67E-49	-1.69E-46
MS.gene052447	MS.gene054175	0.811978	5.34E-51	-1.69E-46
MS.gene054174	MS.gene054175	0.98848	3.50E-174	-1.69E-46
MS.gene054175	MS.gene061245	0.800429	1.45E-48	-1.69E-46
MS.gene054175	MS.gene063846	0.81012	1.35E-50	-1.69E-46
MS.gene054175	MS.gene064718	0.821862	3.22E-53	-1.69E-46
MS.gene054175	MS.gene068185	0.820238	7.62E-53	-1.69E-46
MS.gene054175	MS.gene068474	0.819478	1.14E-52	-1.69E-46
MS.gene054175	MS.gene069223	0.808229	3.43E-50	-1.69E-46
MS.gene054175	MS.gene069271	0.843377	1.46E-58	-1.69E-46
MS.gene054175	MS.gene071058	0.80892	2.44E-50	-1.69E-46
MS.gene054175	MS.gene071806	0.834423	3.02E-56	-1.69E-46
MS.gene054175	MS.gene09504	0.840689	7.47E-58	-1.69E-46
MS.gene054175	MS.gene20657	0.83793	3.89E-57	-1.69E-46
MS.gene054175	MS.gene24211	0.826992	2.00E-54	-1.69E-46
MS.gene054175	MS.gene25539	0.828326	9.57E-55	-1.69E-46
MS.gene054175	MS.gene26056	0.825613	4.26E-54	-1.69E-46
MS.gene054175	MS.gene27717	0.828107	1.08E-54	-1.69E-46
MS.gene054175	MS.gene27720	0.879275	1.42E-69	-1.69E-46
MS.gene054175	MS.gene28508	0.802419	5.66E-49	-1.69E-46
MS.gene054175	MS.gene29654	0.80528	1.44E-49	-1.69E-46
MS.gene054175	MS.gene30073	0.854173	1.47E-61	-1.69E-46
MS.gene054175	MS.gene30139	0.811612	6.42E-51	-1.69E-46
MS.gene054175	MS.gene34615	0.83743	5.22E-57	-1.69E-46
MS.gene054175	MS.gene37264	0.8182	2.22E-52	-1.69E-46
MS.gene054175	MS.gene38429	0.836799	7.57E-57	-1.69E-46
MS.gene054175	MS.gene38850	0.857949	1.15E-62	-1.69E-46
MS.gene054175	MS.gene42573	0.854149	1.49E-61	-1.69E-46
MS.gene054175	MS.gene42887	0.870013	2.01E-66	-1.69E-46
MS.gene054175	MS.gene42891	0.871094	8.88E-67	-1.69E-46
MS.gene054175	MS.gene44575	0.810491	1.12E-50	-1.69E-46
MS.gene054175	MS.gene45794	0.847848	8.92E-60	-1.69E-46
MS.gene054175	MS.gene47943	0.820644	6.15E-53	-1.69E-46
MS.gene054175	MS.gene51252	0.806904	6.56E-50	-1.69E-46
MS.gene054175	MS.gene56618	0.805923	1.06E-49	-1.69E-46
MS.gene054175	MS.gene57443	0.820725	5.89E-53	-1.69E-46
MS.gene054175	MS.gene59780	0.852011	6.10E-61	-1.69E-46
MS.gene054175	MS.gene61898	0.801766	7.71E-49	-1.69E-46
MS.gene054175	MS.gene62053	0.976414	8.84E-142	-1.69E-46
MS.gene054175	MS.gene62748	0.858015	1.10E-62	-1.69E-46
MS.gene054175	MS.gene63142	0.803722	3.04E-49	-1.69E-46
MS.gene054175	MS.gene65816	0.80085	1.19E-48	-1.69E-46
MS.gene054175	MS.gene67182	0.830427	2.95E-55	-1.69E-46
MS.gene054175	MS.gene73540	0.847482	1.12E-59	-1.69E-46
MS.gene054175	MS.gene76061	0.803285	3.75E-49	-1.69E-46
MS.gene054175	MS.gene78647	0.846767	1.77E-59	-1.69E-46
MS.gene054175	MS.gene79147	0.845348	4.30E-59	-1.69E-46
MS.gene054175	MS.gene79824	0.878315	3.10E-69	-1.69E-46
MS.gene054175	MS.gene81140	0.850934	1.23E-60	-1.69E-46
MS.gene054175	MS.gene81193	0.851789	7.06E-61	-1.69E-46
MS.gene054175	MS.gene82668	0.822224	2.66E-53	-1.69E-46
MS.gene054175	MS.gene83109	0.824959	6.08E-54	-1.69E-46
MS.gene054175	MS.gene83166	0.832605	8.59E-56	-1.69E-46
MS.gene054175	MS.gene83609	0.825007	5.93E-54	-1.69E-46
MS.gene054175	MS.gene84001	0.859316	4.50E-63	-1.69E-46
MS.gene054175	MS.gene84968	0.835686	1.45E-56	-1.69E-46
MS.gene054175	MS.gene85079	0.832903	7.25E-56	-1.69E-46
MS.gene054175	MS.gene85361	0.834748	2.50E-56	-1.69E-46
MS.gene054175	MS.gene88533	0.845517	3.87E-59	-1.69E-46
MS.gene054175	MS.gene88539	0.838942	2.13E-57	-1.69E-46
MS.gene054175	MS.gene88548	0.816299	5.94E-52	-1.69E-46
MS.gene054175	MS.gene91451	0.861975	7.03E-64	-1.69E-46
MS.gene054175	MS.gene93832	0.801332	9.47E-49	-1.69E-46
MS.gene054175	MS.gene94237	0.833315	5.72E-56	-1.69E-46
MS.gene054175	MS.gene98550	0.80776	4.32E-50	-1.69E-46
MS.gene054175	MS.gene98673	0.873889	1.04E-67	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 58 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTCGCCTCCATTAAGAAAA+AGG	0.196883	6.1:-28541047	MS.gene054175:CDS
GAAGTAGGGGCGAAGCTTCC+TGG	0.305732	6.1:+28540760	None:intergenic
ACCCTGCTGACAAAATAAAT+TGG	0.306090	6.1:+28540848	None:intergenic
ATAAGAACTTCTTTATTTGC+TGG	0.308076	6.1:+28540987	None:intergenic
ACAGGAAAAGAATGGCGTTC+TGG	0.327901	6.1:+28540936	None:intergenic
TTCCCAGCACTTTCCTGATT+GGG	0.330463	6.1:+28540705	None:intergenic
TGGAAAGCGTCCTCACATTC+TGG	0.348410	6.1:+28541007	None:intergenic
CAACGCCATGAGCACGGTTT+TGG	0.365971	6.1:-28540897	MS.gene054175:CDS
TTTCCCAGCACTTTCCTGAT+TGG	0.367347	6.1:+28540704	None:intergenic
GTCATACTCTGAAAGCTCTA+TGG	0.369364	6.1:+28540649	None:intergenic
ACCATTCTCCCTGCCAAGTC+AGG	0.376615	6.1:+28540680	None:intergenic
CTGGGTTGGTCGCTGAAGGA+TGG	0.391946	6.1:+28540955	None:intergenic
TTGGTCGAAGATTCAGCAAC+AGG	0.406040	6.1:-28540878	MS.gene054175:CDS
CAGGAAAAGAATGGCGTTCT+GGG	0.412313	6.1:+28540937	None:intergenic
TACTCCCTCTTGGTACGAAC+TGG	0.425354	6.1:+28540624	None:intergenic
TCAAATCAGATTACCCAATC+AGG	0.431912	6.1:-28540718	MS.gene054175:CDS
CTGGGAAAACCTGACTTGGC+AGG	0.444702	6.1:-28540689	MS.gene054175:CDS
AAAGGAGGTTGTTCCTCTGC+TGG	0.447721	6.1:+28540557	None:intergenic
ACCTGACTTGGCAGGGAGAA+TGG	0.448765	6.1:-28540681	MS.gene054175:CDS
TGGGTTGGTCGCTGAAGGAT+GGG	0.452250	6.1:+28540956	None:intergenic
TGGCAGGGAGAATGGTGGCC+TGG	0.462166	6.1:-28540673	MS.gene054175:CDS
TCGCTGAAGGATGGGCGGAC+TGG	0.472689	6.1:+28540964	None:intergenic
TGACGACGACTTTGTGACTT+TGG	0.475829	6.1:+28540738	None:intergenic
AAGGAGGTTGTTCCTCTGCT+GGG	0.480377	6.1:+28540558	None:intergenic
CTACAAAGTCTGCCAACACT+TGG	0.484208	6.1:+28540594	None:intergenic
ACTTTGTGACTTTGGAAGTA+GGG	0.493033	6.1:+28540746	None:intergenic
TGTCAGATATCGCCAGGTAC+AGG	0.496623	6.1:+28540918	None:intergenic
TGGGATTTAATACTCCCTCT+TGG	0.506548	6.1:+28540614	None:intergenic
CTCTGAAAGCTCTATGGACC+AGG	0.516584	6.1:+28540655	None:intergenic
TTACCCAATCAGGAAAGTGC+TGG	0.533563	6.1:-28540708	MS.gene054175:CDS
TTCGACCAAAACCGTGCTCA+TGG	0.540082	6.1:+28540892	None:intergenic
TGGCGTTGTCAGATATCGCC+AGG	0.540141	6.1:+28540912	None:intergenic
ATCTGACAACGCCATGAGCA+CGG	0.541033	6.1:-28540903	MS.gene054175:CDS
AAAAGAATGGCGTTCTGGGT+TGG	0.542582	6.1:+28540941	None:intergenic
GACTTTGTGACTTTGGAAGT+AGG	0.543347	6.1:+28540745	None:intergenic
CGTTCTGGGTTGGTCGCTGA+AGG	0.550779	6.1:+28540951	None:intergenic
AGGTTGTTCCTCTGCTGGGG+AGG	0.552428	6.1:+28540562	None:intergenic
TCGCTGTCATCATAACGGCC+AGG	0.564320	6.1:-28540778	MS.gene054175:CDS
TACCCAATCAGGAAAGTGCT+GGG	0.576886	6.1:-28540707	MS.gene054175:CDS
CGCCAGGTACAGGAAAAGAA+TGG	0.578521	6.1:+28540928	None:intergenic
AGTGCTGGGAAAACCTGACT+TGG	0.583617	6.1:-28540693	MS.gene054175:CDS
CTTTGTGACTTTGGAAGTAG+GGG	0.583829	6.1:+28540747	None:intergenic
GTTGGTCGCTGAAGGATGGG+CGG	0.592827	6.1:+28540959	None:intergenic
TACAAAGTCTGCCAACACTT+GGG	0.599588	6.1:+28540595	None:intergenic
TGGGAAAACCTGACTTGGCA+GGG	0.602071	6.1:-28540688	MS.gene054175:CDS
AGGAGGTTGTTCCTCTGCTG+GGG	0.603319	6.1:+28540559	None:intergenic
TTTGTCAGCAGGGTGTTCAG+AGG	0.615325	6.1:-28540839	MS.gene054175:CDS
CGCCCTCGCTGTCATCATAA+CGG	0.617862	6.1:-28540783	MS.gene054175:CDS
GATAAGAGCCATACGAAAGG+AGG	0.621263	6.1:+28540542	None:intergenic
AGAACTCGCCTCCCCAGCAG+AGG	0.628705	6.1:-28540570	MS.gene054175:CDS
GAGTATTAAATCCCAAGTGT+TGG	0.629081	6.1:-28540606	MS.gene054175:CDS
ACGTCCAGTTCGTACCAAGA+GGG	0.634089	6.1:-28540628	MS.gene054175:CDS
CGAGTGGACTCCAGAATGTG+AGG	0.643129	6.1:-28541017	MS.gene054175:CDS
GACGTCCAGTTCGTACCAAG+AGG	0.643966	6.1:-28540629	MS.gene054175:CDS
GGCCGTTATGATGACAGCGA+GGG	0.653340	6.1:+28540781	None:intergenic
AATGGAGGCGAAGAAAGAGA+AGG	0.666696	6.1:+28541057	None:intergenic
TGACTTGGCAGGGAGAATGG+TGG	0.673305	6.1:-28540678	MS.gene054175:CDS
TGGCCGTTATGATGACAGCG+AGG	0.687031	6.1:+28540780	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAAGAACTTCTTTATTTGC+TGG	+	chr6.1:28540631-28540650	None:intergenic	25.0%
!	GAATCTCTCCTTTTTCTTAA+TGG	+	chr6.1:28540579-28540598	None:intergenic	30.0%
	ACCCTGCTGACAAAATAAAT+TGG	+	chr6.1:28540770-28540789	None:intergenic	35.0%
	GAGTATTAAATCCCAAGTGT+TGG	-	chr6.1:28541009-28541028	MS.gene054175:CDS	35.0%
	TCAAATCAGATTACCCAATC+AGG	-	chr6.1:28540897-28540916	MS.gene054175:CDS	35.0%
!	ACTTTGTGACTTTGGAAGTA+GGG	+	chr6.1:28540872-28540891	None:intergenic	35.0%
!	TCTCTCCTTTTTCTTAATGG+AGG	+	chr6.1:28540576-28540595	None:intergenic	35.0%
!!!	AGCCAATTTATTTTGTCAGC+AGG	-	chr6.1:28540765-28540784	MS.gene054175:CDS	35.0%
!!!	GCCAATTTATTTTGTCAGCA+GGG	-	chr6.1:28540766-28540785	MS.gene054175:CDS	35.0%
	AGAAAAAGGAGAGATTCGAG+TGG	-	chr6.1:28540582-28540601	MS.gene054175:CDS	40.0%
	CTTCGCCTCCATTAAGAAAA+AGG	-	chr6.1:28540568-28540587	MS.gene054175:CDS	40.0%
	TACAAAGTCTGCCAACACTT+GGG	+	chr6.1:28541023-28541042	None:intergenic	40.0%
	TGGGATTTAATACTCCCTCT+TGG	+	chr6.1:28541004-28541023	None:intergenic	40.0%
!	CTTTGTGACTTTGGAAGTAG+GGG	+	chr6.1:28540871-28540890	None:intergenic	40.0%
!	GACTTTGTGACTTTGGAAGT+AGG	+	chr6.1:28540873-28540892	None:intergenic	40.0%
!	GTCATACTCTGAAAGCTCTA+TGG	+	chr6.1:28540969-28540988	None:intergenic	40.0%
	AATGGAGGCGAAGAAAGAGA+AGG	+	chr6.1:28540561-28540580	None:intergenic	45.0%
	ACAGGAAAAGAATGGCGTTC+TGG	+	chr6.1:28540682-28540701	None:intergenic	45.0%
	CAGGAAAAGAATGGCGTTCT+GGG	+	chr6.1:28540681-28540700	None:intergenic	45.0%
	CTACAAAGTCTGCCAACACT+TGG	+	chr6.1:28541024-28541043	None:intergenic	45.0%
	TGACGACGACTTTGTGACTT+TGG	+	chr6.1:28540880-28540899	None:intergenic	45.0%
	TTCCCAGCACTTTCCTGATT+GGG	+	chr6.1:28540913-28540932	None:intergenic	45.0%
	TTGGTCGAAGATTCAGCAAC+AGG	-	chr6.1:28540737-28540756	MS.gene054175:CDS	45.0%
	TTTCCCAGCACTTTCCTGAT+TGG	+	chr6.1:28540914-28540933	None:intergenic	45.0%
!	AAAAGAATGGCGTTCTGGGT+TGG	+	chr6.1:28540677-28540696	None:intergenic	45.0%
!	TTACCCAATCAGGAAAGTGC+TGG	-	chr6.1:28540907-28540926	MS.gene054175:CDS	45.0%
!!	TACCCAATCAGGAAAGTGCT+GGG	-	chr6.1:28540908-28540927	MS.gene054175:CDS	45.0%
	AAAGGAGGTTGTTCCTCTGC+TGG	+	chr6.1:28541061-28541080	None:intergenic	50.0%
	AAGGAGGTTGTTCCTCTGCT+GGG	+	chr6.1:28541060-28541079	None:intergenic	50.0%
	ACGTCCAGTTCGTACCAAGA+GGG	-	chr6.1:28540987-28541006	MS.gene054175:CDS	50.0%
	ATCTGACAACGCCATGAGCA+CGG	-	chr6.1:28540712-28540731	MS.gene054175:CDS	50.0%
	CGCCAGGTACAGGAAAAGAA+TGG	+	chr6.1:28540690-28540709	None:intergenic	50.0%
	TACTCCCTCTTGGTACGAAC+TGG	+	chr6.1:28540994-28541013	None:intergenic	50.0%
	TGTCAGATATCGCCAGGTAC+AGG	+	chr6.1:28540700-28540719	None:intergenic	50.0%
	TTCGACCAAAACCGTGCTCA+TGG	+	chr6.1:28540726-28540745	None:intergenic	50.0%
!	AGTGCTGGGAAAACCTGACT+TGG	-	chr6.1:28540922-28540941	MS.gene054175:CDS	50.0%
!	CGCCATTCTTTTCCTGTACC+TGG	-	chr6.1:28540685-28540704	MS.gene054175:CDS	50.0%
!	CTCTGAAAGCTCTATGGACC+AGG	+	chr6.1:28540963-28540982	None:intergenic	50.0%
!	TTTGTCAGCAGGGTGTTCAG+AGG	-	chr6.1:28540776-28540795	MS.gene054175:CDS	50.0%
!!	TGGAAAGCGTCCTCACATTC+TGG	+	chr6.1:28540611-28540630	None:intergenic	50.0%
!!	TGGGAAAACCTGACTTGGCA+GGG	-	chr6.1:28540927-28540946	MS.gene054175:CDS	50.0%
	ACCATTCTCCCTGCCAAGTC+AGG	+	chr6.1:28540938-28540957	None:intergenic	55.0%
	AGGAGGTTGTTCCTCTGCTG+GGG	+	chr6.1:28541059-28541078	None:intergenic	55.0%
	CGCCCTCGCTGTCATCATAA+CGG	-	chr6.1:28540832-28540851	MS.gene054175:CDS	55.0%
	GACGTCCAGTTCGTACCAAG+AGG	-	chr6.1:28540986-28541005	MS.gene054175:CDS	55.0%
	GGCCGTTATGATGACAGCGA+GGG	+	chr6.1:28540837-28540856	None:intergenic	55.0%
	TCGCTGTCATCATAACGGCC+AGG	-	chr6.1:28540837-28540856	MS.gene054175:CDS	55.0%
	TGGCCGTTATGATGACAGCG+AGG	+	chr6.1:28540838-28540857	None:intergenic	55.0%
	TGGCGTTGTCAGATATCGCC+AGG	+	chr6.1:28540706-28540725	None:intergenic	55.0%
!	CAACGCCATGAGCACGGTTT+TGG	-	chr6.1:28540718-28540737	MS.gene054175:CDS	55.0%
!	CGAGTGGACTCCAGAATGTG+AGG	-	chr6.1:28540598-28540617	MS.gene054175:CDS	55.0%
!	TGACTTGGCAGGGAGAATGG+TGG	-	chr6.1:28540937-28540956	MS.gene054175:CDS	55.0%
!!	ACCTGACTTGGCAGGGAGAA+TGG	-	chr6.1:28540934-28540953	MS.gene054175:CDS	55.0%
!!	CTGGGAAAACCTGACTTGGC+AGG	-	chr6.1:28540926-28540945	MS.gene054175:CDS	55.0%
!!	TGGGTTGGTCGCTGAAGGAT+GGG	+	chr6.1:28540662-28540681	None:intergenic	55.0%
	AGGTTGTTCCTCTGCTGGGG+AGG	+	chr6.1:28541056-28541075	None:intergenic	60.0%
	GAAGTAGGGGCGAAGCTTCC+TGG	+	chr6.1:28540858-28540877	None:intergenic	60.0%
	GTTGGTCGCTGAAGGATGGG+CGG	+	chr6.1:28540659-28540678	None:intergenic	60.0%
!!	CGTTCTGGGTTGGTCGCTGA+AGG	+	chr6.1:28540667-28540686	None:intergenic	60.0%
!!	CTGGGTTGGTCGCTGAAGGA+TGG	+	chr6.1:28540663-28540682	None:intergenic	60.0%
	AGAACTCGCCTCCCCAGCAG+AGG	-	chr6.1:28541045-28541064	MS.gene054175:CDS	65.0%
	TCGCTGAAGGATGGGCGGAC+TGG	+	chr6.1:28540654-28540673	None:intergenic	65.0%
	TGGCAGGGAGAATGGTGGCC+TGG	-	chr6.1:28540942-28540961	MS.gene054175:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	28540556	28541081	28540556	ID=MS.gene054175
chr6.1	mRNA	28540556	28541081	28540556	ID=MS.gene054175.t1;Parent=MS.gene054175
chr6.1	exon	28540556	28541081	28540556	ID=MS.gene054175.t1.exon1;Parent=MS.gene054175.t1
chr6.1	CDS	28540556	28541081	28540556	ID=cds.MS.gene054175.t1;Parent=MS.gene054175.t1

Gene Sequence

>MS.gene054175

AGCCTTCTCTTTCTTCGCCTCCATTAAGAAAAAGGAGAGATTCGAGTGGACTCCAGAATGTGAGGACGCTTTCCAGCAAATAAAGAAGTTCTTATCCAGTCCGCCCATCCTTCAGCGACCAACCCAGAACGCCATTCTTTTCCTGTACCTGGCGATATCTGACAACGCCATGAGCACGGTTTTGGTCGAAGATTCAGCAACAGGAGAGAAGCCAATTTATTTTGTCAGCAGGGTGTTCAGAGGAGCAGAGCTGCGATATCAGAAAATAGAACGTCTCGCCCTCGCTGTCATCATAACGGCCAGGAAGCTTCGCCCCTACTTCCAAAGTCACAAAGTCGTCGTCAAATCAGATTACCCAATCAGGAAAGTGCTGGGAAAACCTGACTTGGCAGGGAGAATGGTGGCCTGGTCCATAGAGCTTTCAGAGTATGACGTCCAGTTCGTACCAAGAGGGAGTATTAAATCCCAAGTGTTGGCAGACTTTGTAGTAGAACTCGCCTCCCCAGCAGAGGAACAACCTCCTTTC

Protein sequence

>MS.gene054175.t1

SLLFLRLHUEKGEIRVDSRMUGRFPANKEVLIQSAHPSATNPERHSFPVPGDIUQRHEHGFGRRFSNRREANLFCQQGVQRSRAAISENRTSRPRCHHNGQEASPLLPKSQSRRQIRLPNQESAGKTULGRENGGLVHRAFRVURPVRTKREYUIPSVGRLCSRTRLPSRGTTSF