AlfalfaGEDB | MS.gene054174

M. sativa cultivar XinJiangDaYe / MS.gene054174

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054174.t1	XP_024634530.1	72.4	174	48	0	1	174	872	1045	1.70E-68	268.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054174.t1	A0A392MKB5	66.1	174	59	0	1	174	181	354	5.0e-62	246.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049853	MS.gene054174	0.805534	1.27E-49	-1.69E-46
MS.gene052447	MS.gene054174	0.818709	1.70E-52	-1.69E-46
MS.gene054174	MS.gene054175	0.98848	3.50E-174	-1.69E-46
MS.gene054174	MS.gene061245	0.813523	2.45E-51	-1.69E-46
MS.gene054174	MS.gene063846	0.804448	2.15E-49	-1.69E-46
MS.gene054174	MS.gene064718	0.827009	1.98E-54	-1.69E-46
MS.gene054174	MS.gene068185	0.816589	5.12E-52	-1.69E-46
MS.gene054174	MS.gene068474	0.815256	1.01E-51	-1.69E-46
MS.gene054174	MS.gene069223	0.803513	3.36E-49	-1.69E-46
MS.gene054174	MS.gene069271	0.840341	9.22E-58	-1.69E-46
MS.gene054174	MS.gene071058	0.802902	4.50E-49	-1.69E-46
MS.gene054174	MS.gene071806	0.844439	7.55E-59	-1.69E-46
MS.gene054174	MS.gene08231	0.81179	5.87E-51	-1.69E-46
MS.gene054174	MS.gene09504	0.837256	5.79E-57	-1.69E-46
MS.gene054174	MS.gene20657	0.833122	6.39E-56	-1.69E-46
MS.gene054174	MS.gene24211	0.821385	4.15E-53	-1.69E-46
MS.gene054174	MS.gene25539	0.823077	1.68E-53	-1.69E-46
MS.gene054174	MS.gene26056	0.82079	5.70E-53	-1.69E-46
MS.gene054174	MS.gene27717	0.820901	5.37E-53	-1.69E-46
MS.gene054174	MS.gene27720	0.872083	4.18E-67	-1.69E-46
MS.gene054174	MS.gene28508	0.80954	1.80E-50	-1.69E-46
MS.gene054174	MS.gene29654	0.816345	5.80E-52	-1.69E-46
MS.gene054174	MS.gene30073	0.84929	3.56E-60	-1.69E-46
MS.gene054174	MS.gene30139	0.806482	8.05E-50	-1.69E-46
MS.gene054174	MS.gene34615	0.832516	9.04E-56	-1.69E-46
MS.gene054174	MS.gene37264	0.814441	1.54E-51	-1.69E-46
MS.gene054174	MS.gene38429	0.83318	6.18E-56	-1.69E-46
MS.gene054174	MS.gene38850	0.853807	1.87E-61	-1.69E-46
MS.gene054174	MS.gene42573	0.852262	5.18E-61	-1.69E-46
MS.gene054174	MS.gene42887	0.864543	1.13E-64	-1.69E-46
MS.gene054174	MS.gene42891	0.865358	6.26E-65	-1.69E-46
MS.gene054174	MS.gene44575	0.806586	7.66E-50	-1.69E-46
MS.gene054174	MS.gene45794	0.844056	9.57E-59	-1.69E-46
MS.gene054174	MS.gene47943	0.81914	1.36E-52	-1.69E-46
MS.gene054174	MS.gene51252	0.801544	8.56E-49	-1.69E-46
MS.gene054174	MS.gene56618	0.80094	1.14E-48	-1.69E-46
MS.gene054174	MS.gene57443	0.837245	5.82E-57	-1.69E-46
MS.gene054174	MS.gene59780	0.847465	1.14E-59	-1.69E-46
MS.gene054174	MS.gene61388	0.803967	2.71E-49	-1.69E-46
MS.gene054174	MS.gene62053	0.966308	9.52E-126	-1.69E-46
MS.gene054174	MS.gene62748	0.852765	3.72E-61	-1.69E-46
MS.gene054174	MS.gene63142	0.800205	1.61E-48	-1.69E-46
MS.gene054174	MS.gene67182	0.827681	1.37E-54	-1.69E-46
MS.gene054174	MS.gene73540	0.841852	3.69E-58	-1.69E-46
MS.gene054174	MS.gene76061	0.808292	3.33E-50	-1.69E-46
MS.gene054174	MS.gene78647	0.841897	3.60E-58	-1.69E-46
MS.gene054174	MS.gene79147	0.839681	1.37E-57	-1.69E-46
MS.gene054174	MS.gene79824	0.873274	1.67E-67	-1.69E-46
MS.gene054174	MS.gene81140	0.843744	1.16E-58	-1.69E-46
MS.gene054174	MS.gene81193	0.844856	5.83E-59	-1.69E-46
MS.gene054174	MS.gene82668	0.819725	9.99E-53	-1.69E-46
MS.gene054174	MS.gene83109	0.820833	5.57E-53	-1.69E-46
MS.gene054174	MS.gene83166	0.832503	9.11E-56	-1.69E-46
MS.gene054174	MS.gene83609	0.836074	1.16E-56	-1.69E-46
MS.gene054174	MS.gene84001	0.854866	9.25E-62	-1.69E-46
MS.gene054174	MS.gene84968	0.830381	3.03E-55	-1.69E-46
MS.gene054174	MS.gene85079	0.844881	5.74E-59	-1.69E-46
MS.gene054174	MS.gene85361	0.828768	7.48E-55	-1.69E-46
MS.gene054174	MS.gene88533	0.856318	3.49E-62	-1.69E-46
MS.gene054174	MS.gene88539	0.84995	2.33E-60	-1.69E-46
MS.gene054174	MS.gene88548	0.826956	2.04E-54	-1.69E-46
MS.gene054174	MS.gene91451	0.85867	7.02E-63	-1.69E-46
MS.gene054174	MS.gene93832	0.811972	5.36E-51	-1.69E-46
MS.gene054174	MS.gene94237	0.839084	1.96E-57	-1.69E-46
MS.gene054174	MS.gene98550	0.802341	5.87E-49	-1.69E-46
MS.gene054174	MS.gene98673	0.868615	5.72E-66	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 58 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTCGCCTCCATTAAGAAAA+AGG	0.196883	6.1:-28528511	MS.gene054174:CDS
GAAGTAGGGGCGAAGCTTCC+TGG	0.305732	6.1:+28528224	None:intergenic
ACCCTGCTGACAAAATAAAT+TGG	0.306090	6.1:+28528312	None:intergenic
ATAAGAACTTCTTTATTTGC+TGG	0.308076	6.1:+28528451	None:intergenic
ACAGGAAAAGAATGGCGTTC+TGG	0.327901	6.1:+28528400	None:intergenic
TTCCCAGCACTTTCCTGATT+GGG	0.330463	6.1:+28528169	None:intergenic
TGGAAAGCGTCCTCACATTC+TGG	0.348410	6.1:+28528471	None:intergenic
CAACGCCATGAGCACGGTTT+TGG	0.365971	6.1:-28528361	MS.gene054174:CDS
TTTCCCAGCACTTTCCTGAT+TGG	0.367347	6.1:+28528168	None:intergenic
GTCATACTCTGAAAGCTCTA+TGG	0.369364	6.1:+28528113	None:intergenic
ACCATTCTCCCTGCCAAGTC+AGG	0.376615	6.1:+28528144	None:intergenic
CTGGGTTGGTCGCTGAAGGA+TGG	0.391946	6.1:+28528419	None:intergenic
TTGGTCGAAGATTCAGCAAC+AGG	0.406040	6.1:-28528342	MS.gene054174:CDS
CAGGAAAAGAATGGCGTTCT+GGG	0.412313	6.1:+28528401	None:intergenic
TACTCCCTCTTGGTACGAAC+TGG	0.425354	6.1:+28528088	None:intergenic
TCAAATCAGATTACCCAATC+AGG	0.431912	6.1:-28528182	MS.gene054174:CDS
CTGGGAAAACCTGACTTGGC+AGG	0.444702	6.1:-28528153	MS.gene054174:CDS
AAAGGAGGTTGTTCCTCTGC+TGG	0.447721	6.1:+28528021	None:intergenic
ACCTGACTTGGCAGGGAGAA+TGG	0.448765	6.1:-28528145	MS.gene054174:CDS
TGGGTTGGTCGCTGAAGGAT+GGG	0.452250	6.1:+28528420	None:intergenic
TGGCAGGGAGAATGGTGGCC+TGG	0.462166	6.1:-28528137	MS.gene054174:CDS
TCGCTGAAGGATGGGCGGAC+TGG	0.472689	6.1:+28528428	None:intergenic
TGACGACGACTTTGTGACTT+TGG	0.475829	6.1:+28528202	None:intergenic
AAGGAGGTTGTTCCTCTGCT+GGG	0.480377	6.1:+28528022	None:intergenic
CTACAAAGTCTGCCAACACT+TGG	0.484208	6.1:+28528058	None:intergenic
ACTTTGTGACTTTGGAAGTA+GGG	0.493033	6.1:+28528210	None:intergenic
TGTCAGATATCGCCAGGTAC+AGG	0.496623	6.1:+28528382	None:intergenic
TGGGATTTAATACTCCCTCT+TGG	0.506548	6.1:+28528078	None:intergenic
CTCTGAAAGCTCTATGGACC+AGG	0.516584	6.1:+28528119	None:intergenic
TTACCCAATCAGGAAAGTGC+TGG	0.533563	6.1:-28528172	MS.gene054174:CDS
TTCGACCAAAACCGTGCTCA+TGG	0.540082	6.1:+28528356	None:intergenic
TGGCGTTGTCAGATATCGCC+AGG	0.540141	6.1:+28528376	None:intergenic
ATCTGACAACGCCATGAGCA+CGG	0.541033	6.1:-28528367	MS.gene054174:CDS
AAAAGAATGGCGTTCTGGGT+TGG	0.542582	6.1:+28528405	None:intergenic
GACTTTGTGACTTTGGAAGT+AGG	0.543347	6.1:+28528209	None:intergenic
CGTTCTGGGTTGGTCGCTGA+AGG	0.550779	6.1:+28528415	None:intergenic
AGGTTGTTCCTCTGCTGGGG+AGG	0.552428	6.1:+28528026	None:intergenic
TCGCTGTCATCATAACGGCC+AGG	0.564320	6.1:-28528242	MS.gene054174:CDS
TACCCAATCAGGAAAGTGCT+GGG	0.576886	6.1:-28528171	MS.gene054174:CDS
CGCCAGGTACAGGAAAAGAA+TGG	0.578521	6.1:+28528392	None:intergenic
AGTGCTGGGAAAACCTGACT+TGG	0.583617	6.1:-28528157	MS.gene054174:CDS
CTTTGTGACTTTGGAAGTAG+GGG	0.583829	6.1:+28528211	None:intergenic
GTTGGTCGCTGAAGGATGGG+CGG	0.592827	6.1:+28528423	None:intergenic
TACAAAGTCTGCCAACACTT+GGG	0.599588	6.1:+28528059	None:intergenic
TGGGAAAACCTGACTTGGCA+GGG	0.602071	6.1:-28528152	MS.gene054174:CDS
AGGAGGTTGTTCCTCTGCTG+GGG	0.603319	6.1:+28528023	None:intergenic
TTTGTCAGCAGGGTGTTCAG+AGG	0.615325	6.1:-28528303	MS.gene054174:CDS
CGCCCTCGCTGTCATCATAA+CGG	0.617862	6.1:-28528247	MS.gene054174:CDS
GATAAGAGCCATACGAAAGG+AGG	0.621263	6.1:+28528006	None:intergenic
AGAACTCGCCTCCCCAGCAG+AGG	0.628705	6.1:-28528034	MS.gene054174:CDS
GAGTATTAAATCCCAAGTGT+TGG	0.629081	6.1:-28528070	MS.gene054174:CDS
ACGTCCAGTTCGTACCAAGA+GGG	0.634089	6.1:-28528092	MS.gene054174:CDS
CGAGTGGACTCCAGAATGTG+AGG	0.643129	6.1:-28528481	MS.gene054174:CDS
GACGTCCAGTTCGTACCAAG+AGG	0.643966	6.1:-28528093	MS.gene054174:CDS
GGCCGTTATGATGACAGCGA+GGG	0.653340	6.1:+28528245	None:intergenic
AATGGAGGCGAAGAAAGAGA+AGG	0.666696	6.1:+28528521	None:intergenic
TGACTTGGCAGGGAGAATGG+TGG	0.673305	6.1:-28528142	MS.gene054174:CDS
TGGCCGTTATGATGACAGCG+AGG	0.687031	6.1:+28528244	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAAGAACTTCTTTATTTGC+TGG	+	chr6.1:28528095-28528114	None:intergenic	25.0%
!	GAATCTCTCCTTTTTCTTAA+TGG	+	chr6.1:28528043-28528062	None:intergenic	30.0%
	ACCCTGCTGACAAAATAAAT+TGG	+	chr6.1:28528234-28528253	None:intergenic	35.0%
	GAGTATTAAATCCCAAGTGT+TGG	-	chr6.1:28528473-28528492	MS.gene054174:CDS	35.0%
	TCAAATCAGATTACCCAATC+AGG	-	chr6.1:28528361-28528380	MS.gene054174:CDS	35.0%
!	ACTTTGTGACTTTGGAAGTA+GGG	+	chr6.1:28528336-28528355	None:intergenic	35.0%
!	TCTCTCCTTTTTCTTAATGG+AGG	+	chr6.1:28528040-28528059	None:intergenic	35.0%
!!!	AGCCAATTTATTTTGTCAGC+AGG	-	chr6.1:28528229-28528248	MS.gene054174:CDS	35.0%
!!!	GCCAATTTATTTTGTCAGCA+GGG	-	chr6.1:28528230-28528249	MS.gene054174:CDS	35.0%
	AGAAAAAGGAGAGATTCGAG+TGG	-	chr6.1:28528046-28528065	MS.gene054174:CDS	40.0%
	CTTCGCCTCCATTAAGAAAA+AGG	-	chr6.1:28528032-28528051	MS.gene054174:CDS	40.0%
	TACAAAGTCTGCCAACACTT+GGG	+	chr6.1:28528487-28528506	None:intergenic	40.0%
	TGGGATTTAATACTCCCTCT+TGG	+	chr6.1:28528468-28528487	None:intergenic	40.0%
!	CTTTGTGACTTTGGAAGTAG+GGG	+	chr6.1:28528335-28528354	None:intergenic	40.0%
!	GACTTTGTGACTTTGGAAGT+AGG	+	chr6.1:28528337-28528356	None:intergenic	40.0%
!	GTCATACTCTGAAAGCTCTA+TGG	+	chr6.1:28528433-28528452	None:intergenic	40.0%
	AATGGAGGCGAAGAAAGAGA+AGG	+	chr6.1:28528025-28528044	None:intergenic	45.0%
	ACAGGAAAAGAATGGCGTTC+TGG	+	chr6.1:28528146-28528165	None:intergenic	45.0%
	CAGGAAAAGAATGGCGTTCT+GGG	+	chr6.1:28528145-28528164	None:intergenic	45.0%
	CTACAAAGTCTGCCAACACT+TGG	+	chr6.1:28528488-28528507	None:intergenic	45.0%
	TGACGACGACTTTGTGACTT+TGG	+	chr6.1:28528344-28528363	None:intergenic	45.0%
	TTCCCAGCACTTTCCTGATT+GGG	+	chr6.1:28528377-28528396	None:intergenic	45.0%
	TTGGTCGAAGATTCAGCAAC+AGG	-	chr6.1:28528201-28528220	MS.gene054174:CDS	45.0%
	TTTCCCAGCACTTTCCTGAT+TGG	+	chr6.1:28528378-28528397	None:intergenic	45.0%
!	AAAAGAATGGCGTTCTGGGT+TGG	+	chr6.1:28528141-28528160	None:intergenic	45.0%
!	TTACCCAATCAGGAAAGTGC+TGG	-	chr6.1:28528371-28528390	MS.gene054174:CDS	45.0%
!!	TACCCAATCAGGAAAGTGCT+GGG	-	chr6.1:28528372-28528391	MS.gene054174:CDS	45.0%
	AAAGGAGGTTGTTCCTCTGC+TGG	+	chr6.1:28528525-28528544	None:intergenic	50.0%
	AAGGAGGTTGTTCCTCTGCT+GGG	+	chr6.1:28528524-28528543	None:intergenic	50.0%
	ACGTCCAGTTCGTACCAAGA+GGG	-	chr6.1:28528451-28528470	MS.gene054174:CDS	50.0%
	ATCTGACAACGCCATGAGCA+CGG	-	chr6.1:28528176-28528195	MS.gene054174:CDS	50.0%
	CGCCAGGTACAGGAAAAGAA+TGG	+	chr6.1:28528154-28528173	None:intergenic	50.0%
	TACTCCCTCTTGGTACGAAC+TGG	+	chr6.1:28528458-28528477	None:intergenic	50.0%
	TGTCAGATATCGCCAGGTAC+AGG	+	chr6.1:28528164-28528183	None:intergenic	50.0%
	TTCGACCAAAACCGTGCTCA+TGG	+	chr6.1:28528190-28528209	None:intergenic	50.0%
!	AGTGCTGGGAAAACCTGACT+TGG	-	chr6.1:28528386-28528405	MS.gene054174:CDS	50.0%
!	CGCCATTCTTTTCCTGTACC+TGG	-	chr6.1:28528149-28528168	MS.gene054174:CDS	50.0%
!	CTCTGAAAGCTCTATGGACC+AGG	+	chr6.1:28528427-28528446	None:intergenic	50.0%
!	TTTGTCAGCAGGGTGTTCAG+AGG	-	chr6.1:28528240-28528259	MS.gene054174:CDS	50.0%
!!	TGGAAAGCGTCCTCACATTC+TGG	+	chr6.1:28528075-28528094	None:intergenic	50.0%
!!	TGGGAAAACCTGACTTGGCA+GGG	-	chr6.1:28528391-28528410	MS.gene054174:CDS	50.0%
	ACCATTCTCCCTGCCAAGTC+AGG	+	chr6.1:28528402-28528421	None:intergenic	55.0%
	AGGAGGTTGTTCCTCTGCTG+GGG	+	chr6.1:28528523-28528542	None:intergenic	55.0%
	CGCCCTCGCTGTCATCATAA+CGG	-	chr6.1:28528296-28528315	MS.gene054174:CDS	55.0%
	GACGTCCAGTTCGTACCAAG+AGG	-	chr6.1:28528450-28528469	MS.gene054174:CDS	55.0%
	GGCCGTTATGATGACAGCGA+GGG	+	chr6.1:28528301-28528320	None:intergenic	55.0%
	TCGCTGTCATCATAACGGCC+AGG	-	chr6.1:28528301-28528320	MS.gene054174:CDS	55.0%
	TGGCCGTTATGATGACAGCG+AGG	+	chr6.1:28528302-28528321	None:intergenic	55.0%
	TGGCGTTGTCAGATATCGCC+AGG	+	chr6.1:28528170-28528189	None:intergenic	55.0%
!	CAACGCCATGAGCACGGTTT+TGG	-	chr6.1:28528182-28528201	MS.gene054174:CDS	55.0%
!	CGAGTGGACTCCAGAATGTG+AGG	-	chr6.1:28528062-28528081	MS.gene054174:CDS	55.0%
!	TGACTTGGCAGGGAGAATGG+TGG	-	chr6.1:28528401-28528420	MS.gene054174:CDS	55.0%
!!	ACCTGACTTGGCAGGGAGAA+TGG	-	chr6.1:28528398-28528417	MS.gene054174:CDS	55.0%
!!	CTGGGAAAACCTGACTTGGC+AGG	-	chr6.1:28528390-28528409	MS.gene054174:CDS	55.0%
!!	TGGGTTGGTCGCTGAAGGAT+GGG	+	chr6.1:28528126-28528145	None:intergenic	55.0%
	AGGTTGTTCCTCTGCTGGGG+AGG	+	chr6.1:28528520-28528539	None:intergenic	60.0%
	GAAGTAGGGGCGAAGCTTCC+TGG	+	chr6.1:28528322-28528341	None:intergenic	60.0%
	GTTGGTCGCTGAAGGATGGG+CGG	+	chr6.1:28528123-28528142	None:intergenic	60.0%
!!	CGTTCTGGGTTGGTCGCTGA+AGG	+	chr6.1:28528131-28528150	None:intergenic	60.0%
!!	CTGGGTTGGTCGCTGAAGGA+TGG	+	chr6.1:28528127-28528146	None:intergenic	60.0%
	AGAACTCGCCTCCCCAGCAG+AGG	-	chr6.1:28528509-28528528	MS.gene054174:CDS	65.0%
	TCGCTGAAGGATGGGCGGAC+TGG	+	chr6.1:28528118-28528137	None:intergenic	65.0%
	TGGCAGGGAGAATGGTGGCC+TGG	-	chr6.1:28528406-28528425	MS.gene054174:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	28528020	28528545	28528020	ID=MS.gene054174
chr6.1	mRNA	28528020	28528545	28528020	ID=MS.gene054174.t1;Parent=MS.gene054174
chr6.1	exon	28528020	28528545	28528020	ID=MS.gene054174.t1.exon1;Parent=MS.gene054174.t1
chr6.1	CDS	28528020	28528545	28528020	ID=cds.MS.gene054174.t1;Parent=MS.gene054174.t1

Gene Sequence

>MS.gene054174

AGCCTTCTCTTTCTTCGCCTCCATTAAGAAAAAGGAGAGATTCGAGTGGACTCCAGAATGTGAGGACGCTTTCCAGCAAATAAAGAAGTTCTTATCCAGTCCGCCCATCCTTCAGCGACCAACCCAGAACGCCATTCTTTTCCTGTACCTGGCGATATCTGACAACGCCATGAGCACGGTTTTGGTCGAAGATTCAGCAACAGGAGAGAAGCCAATTTATTTTGTCAGCAGGGTGTTCAGAGGAGCAGAGCTGCGATATCAGAAAATAGAACGTCTCGCCCTCGCTGTCATCATAACGGCCAGGAAGCTTCGCCCCTACTTCCAAAGTCACAAAGTCGTCGTCAAATCAGATTACCCAATCAGGAAAGTGCTGGGAAAACCTGACTTGGCAGGGAGAATGGTGGCCTGGTCCATAGAGCTTTCAGAGTATGACGTCCAGTTCGTACCAAGAGGGAGTATTAAATCCCAAGTGTTGGCAGACTTTGTAGTAGAACTCGCCTCCCCAGCAGAGGAACAACCTCCTTTC

Protein sequence

>MS.gene054174.t1

SLLFLRLHUEKGEIRVDSRMUGRFPANKEVLIQSAHPSATNPERHSFPVPGDIUQRHEHGFGRRFSNRREANLFCQQGVQRSRAAISENRTSRPRCHHNGQEASPLLPKSQSRRQIRLPNQESAGKTULGRENGGLVHRAFRVURPVRTKREYUIPSVGRLCSRTRLPSRGTTSF