Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37500.t1 | XP_003627654.1 | 95.7 | 513 | 15 | 1 | 218 | 723 | 1 | 513 | 3.00E-244 | 854.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37500.t1 | Q9SWE5 | 79.1 | 196 | 40 | 1 | 12 | 207 | 9 | 203 | 2.4e-93 | 344.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37500.t1 | G7LCR3 | 95.7 | 513 | 15 | 1 | 218 | 723 | 1 | 513 | 2.2e-244 | 854.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050073 | MS.gene37500 | 0.805723 | 1.16E-49 | -1.69E-46 |
| MS.gene056173 | MS.gene37500 | 0.849303 | 3.53E-60 | -1.69E-46 |
| MS.gene056174 | MS.gene37500 | 0.815089 | 1.11E-51 | -1.69E-46 |
| MS.gene057869 | MS.gene37500 | 0.814114 | 1.82E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37500.t1 | MTR_8g035590 | 97.271 | 513 | 7 | 1 | 218 | 723 | 1 | 513 | 0.0 | 997 |
| MS.gene37500.t1 | MTR_7g075790 | 80.971 | 515 | 88 | 3 | 218 | 723 | 1 | 514 | 0.0 | 828 |
| MS.gene37500.t1 | MTR_8g035610 | 99.043 | 209 | 2 | 0 | 1 | 209 | 2 | 210 | 2.21e-150 | 436 |
| MS.gene37500.t1 | MTR_8g035610 | 98.980 | 196 | 2 | 0 | 14 | 209 | 1 | 196 | 2.57e-140 | 410 |
| MS.gene37500.t1 | MTR_4g115660 | 38.718 | 390 | 228 | 7 | 232 | 616 | 15 | 398 | 3.55e-85 | 280 |
| MS.gene37500.t1 | MTR_4g115660 | 44.231 | 104 | 55 | 2 | 480 | 580 | 440 | 543 | 1.62e-15 | 80.5 |
| MS.gene37500.t1 | MTR_3g106660 | 39.312 | 407 | 227 | 10 | 218 | 617 | 1 | 394 | 5.43e-82 | 271 |
| MS.gene37500.t1 | MTR_3g106660 | 40.206 | 97 | 57 | 1 | 480 | 575 | 439 | 535 | 4.60e-13 | 72.8 |
| MS.gene37500.t1 | MTR_7g075760 | 55.238 | 210 | 88 | 3 | 7 | 212 | 4 | 211 | 2.71e-75 | 242 |
| MS.gene37500.t1 | MTR_7g076050 | 55.238 | 210 | 88 | 3 | 7 | 212 | 4 | 211 | 2.71e-75 | 242 |
| MS.gene37500.t1 | MTR_7g076070 | 56.338 | 213 | 80 | 3 | 1 | 209 | 2 | 205 | 4.47e-73 | 236 |
| MS.gene37500.t1 | MTR_7g075710 | 56.338 | 213 | 80 | 3 | 1 | 209 | 1 | 204 | 8.65e-73 | 235 |
| MS.gene37500.t1 | MTR_7g075750 | 57.360 | 197 | 79 | 2 | 14 | 209 | 1 | 193 | 1.75e-68 | 223 |
| MS.gene37500.t1 | MTR_4g073400 | 31.343 | 402 | 255 | 11 | 231 | 624 | 18 | 406 | 1.11e-57 | 205 |
| MS.gene37500.t1 | MTR_1g094810 | 32.312 | 359 | 228 | 10 | 244 | 596 | 30 | 379 | 4.46e-54 | 195 |
| MS.gene37500.t1 | MTR_1g025550 | 31.951 | 410 | 250 | 12 | 218 | 613 | 1 | 395 | 9.48e-52 | 188 |
| MS.gene37500.t1 | MTR_7g076045 | 59.231 | 130 | 47 | 2 | 75 | 204 | 5 | 128 | 5.64e-43 | 152 |
| MS.gene37500.t1 | MTR_7g074480 | 60.784 | 51 | 15 | 2 | 109 | 159 | 3 | 48 | 1.03e-11 | 62.8 |
| MS.gene37500.t1 | MTR_2g072180 | 24.523 | 367 | 238 | 13 | 285 | 629 | 74 | 423 | 1.21e-11 | 68.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37500.t1 | AT3G61050 | 75.728 | 515 | 108 | 4 | 218 | 721 | 1 | 509 | 0.0 | 754 |
| MS.gene37500.t1 | AT3G61050 | 75.728 | 515 | 108 | 4 | 218 | 721 | 1 | 509 | 0.0 | 754 |
| MS.gene37500.t1 | AT3G61030 | 61.207 | 348 | 68 | 8 | 235 | 582 | 1 | 281 | 2.41e-126 | 385 |
| MS.gene37500.t1 | AT3G60950 | 60.057 | 348 | 79 | 6 | 235 | 582 | 1 | 288 | 2.30e-125 | 387 |
| MS.gene37500.t1 | AT3G18030 | 79.082 | 196 | 40 | 1 | 12 | 207 | 9 | 203 | 4.14e-117 | 350 |
| MS.gene37500.t1 | AT1G48605 | 76.650 | 197 | 45 | 1 | 13 | 209 | 2 | 197 | 6.48e-113 | 339 |
| MS.gene37500.t1 | AT1G05500 | 40.050 | 397 | 227 | 7 | 218 | 609 | 1 | 391 | 6.97e-95 | 305 |
| MS.gene37500.t1 | AT1G05500 | 46.250 | 80 | 41 | 2 | 480 | 557 | 434 | 513 | 4.57e-13 | 72.8 |
| MS.gene37500.t1 | AT5G04220 | 34.959 | 369 | 225 | 10 | 278 | 638 | 62 | 423 | 6.67e-59 | 208 |
| MS.gene37500.t1 | AT5G04220 | 33.676 | 389 | 231 | 11 | 282 | 663 | 4 | 372 | 2.41e-58 | 205 |
| MS.gene37500.t1 | AT1G20080 | 31.773 | 406 | 256 | 12 | 218 | 613 | 1 | 395 | 2.20e-55 | 199 |
| MS.gene37500.t1 | AT1G20080 | 31.773 | 406 | 256 | 12 | 218 | 613 | 1 | 395 | 2.20e-55 | 199 |
| MS.gene37500.t1 | AT1G20080 | 31.773 | 406 | 256 | 12 | 218 | 613 | 1 | 395 | 2.20e-55 | 199 |
| MS.gene37500.t1 | AT1G20080 | 31.773 | 406 | 256 | 12 | 218 | 613 | 1 | 395 | 2.20e-55 | 199 |
| MS.gene37500.t1 | AT1G20080 | 31.773 | 406 | 256 | 12 | 218 | 613 | 1 | 395 | 2.20e-55 | 199 |
| MS.gene37500.t1 | AT1G05500 | 40.316 | 253 | 141 | 6 | 361 | 609 | 1 | 247 | 1.20e-54 | 194 |
| MS.gene37500.t1 | AT1G05500 | 46.250 | 80 | 41 | 2 | 480 | 557 | 290 | 369 | 4.48e-13 | 72.4 |
| MS.gene37500.t1 | AT2G20990 | 28.668 | 443 | 294 | 11 | 218 | 646 | 1 | 435 | 2.65e-51 | 187 |
| MS.gene37500.t1 | AT2G20990 | 26.981 | 467 | 295 | 11 | 218 | 646 | 1 | 459 | 4.02e-47 | 176 |
| MS.gene37500.t1 | AT2G20990 | 27.500 | 480 | 288 | 14 | 218 | 645 | 1 | 472 | 3.66e-45 | 171 |
| MS.gene37500.t1 | AT5G04220 | 31.718 | 227 | 133 | 7 | 442 | 663 | 3 | 212 | 1.51e-20 | 93.6 |
| MS.gene37500.t1 | AT3G14590 | 25.733 | 307 | 203 | 11 | 285 | 570 | 70 | 372 | 4.27e-13 | 73.2 |
| MS.gene37500.t1 | AT3G14590 | 25.733 | 307 | 203 | 11 | 285 | 570 | 39 | 341 | 4.56e-13 | 73.2 |
| MS.gene37500.t1 | AT3G14590 | 25.733 | 307 | 203 | 11 | 285 | 570 | 25 | 327 | 4.90e-13 | 73.2 |
| MS.gene37500.t1 | AT1G53590 | 25.338 | 296 | 199 | 10 | 285 | 561 | 70 | 362 | 3.69e-12 | 70.5 |
| MS.gene37500.t1 | AT1G50260 | 23.164 | 354 | 240 | 11 | 285 | 614 | 190 | 535 | 2.56e-11 | 67.4 |
| MS.gene37500.t1 | AT3G19830 | 23.125 | 320 | 221 | 9 | 285 | 581 | 202 | 519 | 2.84e-11 | 67.4 |
| MS.gene37500.t1 | AT3G19830 | 23.125 | 320 | 221 | 9 | 285 | 581 | 202 | 519 | 2.84e-11 | 67.4 |
| MS.gene37500.t1 | AT3G19830 | 23.125 | 320 | 221 | 9 | 285 | 581 | 202 | 519 | 2.84e-11 | 67.4 |
| MS.gene37500.t1 | AT3G19830 | 23.125 | 320 | 221 | 9 | 285 | 581 | 202 | 519 | 2.84e-11 | 67.4 |
Find 192 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTCACTATAGTCACTTTA+AGG | 0.123179 | 8.3:-60195066 | None:intergenic |
| GTGAAGATGGGTTTGATTTC+TGG | 0.174093 | 8.3:+60181440 | MS.gene37500:CDS |
| ATTGTTCCTCTTTGTTAAAT+TGG | 0.216095 | 8.3:-60197458 | None:intergenic |
| TGGTGATCCCTGTATTATTT+TGG | 0.224332 | 8.3:+60193504 | MS.gene37500:CDS |
| TAGCAAGGCAGGAAAGTTTA+TGG | 0.234578 | 8.3:+60197689 | MS.gene37500:CDS |
| GGACTTGTTGGCAGTGGTTT+TGG | 0.244235 | 8.3:+60197648 | MS.gene37500:CDS |
| CCAACTGAACTCACAGGTTC+TGG | 0.249432 | 8.3:-60169056 | None:intergenic |
| GGAATCTGTTGAACCTCTTC+TGG | 0.253712 | 8.3:+60191931 | MS.gene37500:CDS |
| AGGTTAGCTTGTGGGGATTA+TGG | 0.262541 | 8.3:+60170769 | MS.gene37500:CDS |
| GAGCCTTACAGTAGAGTAAA+TGG | 0.285370 | 8.3:-60170818 | None:intergenic |
| GAGTAAAATCCGAGGCTTCC+TGG | 0.300240 | 8.3:-60169108 | None:intergenic |
| GCGGTATCCCTGTTGATATT+AGG | 0.312854 | 8.3:+60194906 | MS.gene37500:CDS |
| AAAATTACCCCTTATTGAAT+TGG | 0.312912 | 8.3:+60196611 | MS.gene37500:CDS |
| GTCCATTATGATTTGACCTT+TGG | 0.317086 | 8.3:-60193464 | None:intergenic |
| TGAAGATGGGTTTGATTTCT+GGG | 0.322511 | 8.3:+60181441 | MS.gene37500:CDS |
| TTAGTAATACCTTCAACTTT+TGG | 0.323824 | 8.3:-60192019 | None:intergenic |
| ACAACAACTTGAATCCTGTT+TGG | 0.333128 | 8.3:+60195185 | MS.gene37500:CDS |
| GTTCCACATAAAAGTGTTCA+TGG | 0.333551 | 8.3:-60170674 | None:intergenic |
| TTTGTACTTCCAATTCAATA+AGG | 0.338968 | 8.3:-60196620 | None:intergenic |
| GTTCATAATCCACAGGAAAA+TGG | 0.339237 | 8.3:+60197765 | MS.gene37500:CDS |
| CAACAACTTGAATCCTGTTT+GGG | 0.344781 | 8.3:+60195186 | MS.gene37500:CDS |
| TTTGTCATTGTTTCTCTGAA+TGG | 0.349362 | 8.3:+60169171 | MS.gene37500:CDS |
| CTTTAAATCTTCTCTGTTGA+GGG | 0.353969 | 8.3:-60184746 | None:intergenic |
| CATATTTACCTAATATCAAC+AGG | 0.355808 | 8.3:-60194914 | None:intergenic |
| GATCACTGCATGTACCTTTA+CGG | 0.356358 | 8.3:-60196724 | None:intergenic |
| GGAAGTTTAACTGCTCTTCC+TGG | 0.356926 | 8.3:+60194712 | MS.gene37500:CDS |
| CTGAAAGCCGCCGGCGTCAT+AGG | 0.357515 | 8.3:+60197528 | MS.gene37500:CDS |
| CTTCTTATCTTTCACCTTGA+GGG | 0.358015 | 8.3:-60196685 | None:intergenic |
| CTTTGTTAAATTGGTAATAC+AGG | 0.360114 | 8.3:-60197449 | None:intergenic |
| AGTGGCATTGGCGCTGGAGC+TGG | 0.362210 | 8.3:+60197627 | MS.gene37500:CDS |
| TTGTCATTGTTTCTCTGAAT+GGG | 0.368648 | 8.3:+60169172 | MS.gene37500:CDS |
| ACAGGAAAAGATATCCATTC+AGG | 0.370019 | 8.3:-60184790 | None:intergenic |
| TTGTACTTCCAATTCAATAA+GGG | 0.372498 | 8.3:-60196619 | None:intergenic |
| TGTTTGCTGTTGTAGATTGC+TGG | 0.375544 | 8.3:+60170577 | MS.gene37500:intron |
| GTATACAAAATCACATCTTT+AGG | 0.376959 | 8.3:-60169254 | None:intergenic |
| GTATAATCAATTCTCGGCTT+TGG | 0.377247 | 8.3:-60194673 | None:intergenic |
| TTCAGAGTATAATCAATTCT+CGG | 0.384703 | 8.3:-60194679 | None:intergenic |
| TCACATGTATCGTGCGAGCC+TGG | 0.385273 | 8.3:+60170620 | MS.gene37500:CDS |
| CAAGCTAACCTCTTTGTAAC+AGG | 0.386122 | 8.3:-60170757 | None:intergenic |
| GGCGCTGGAGCTGGACTTGT+TGG | 0.387957 | 8.3:+60197636 | MS.gene37500:CDS |
| GCTATTTGGCATAGCATTGA+TGG | 0.388207 | 8.3:+60181478 | MS.gene37500:CDS |
| TGAACCACTATGACCTGTTA+TGG | 0.394539 | 8.3:-60197718 | None:intergenic |
| ACTGGCATTGGTGCCGGAGC+TGG | 0.397729 | 8.3:+60197594 | MS.gene37500:CDS |
| AACTTCAATGAAGTAATTCC+AGG | 0.401415 | 8.3:-60191968 | None:intergenic |
| AGCAAGGCAGGAAAGTTTAT+GGG | 0.401661 | 8.3:+60197690 | MS.gene37500:CDS |
| GAAGATTTAAAGAAAATTTG+TGG | 0.419290 | 8.3:+60184757 | MS.gene37500:CDS |
| GTATATTCGACCTTTATTCA+AGG | 0.419952 | 8.3:+60195144 | MS.gene37500:CDS |
| TGTTGCATCAATCCCTATCC+AGG | 0.420271 | 8.3:+60193543 | MS.gene37500:CDS |
| TTCGACAGCCAAAATAATAC+AGG | 0.421110 | 8.3:-60193512 | None:intergenic |
| TACCAAAGGTCAAATCATAA+TGG | 0.422939 | 8.3:+60193462 | MS.gene37500:CDS |
| CCATACCTTTGCAATTCTCT+TGG | 0.424354 | 8.3:-60181534 | None:intergenic |
| TTTATGGGAAGGACCATAAC+AGG | 0.424952 | 8.3:+60197705 | MS.gene37500:CDS |
| ATTTAACAAAGAGGAACAAT+TGG | 0.429158 | 8.3:+60197461 | MS.gene37500:CDS |
| TTCACATTGAGCTTCGCAAA+TGG | 0.429618 | 8.3:+60169324 | MS.gene37500:CDS |
| AGTCTCACCTGCTCATATAC+AGG | 0.436315 | 8.3:-60184808 | None:intergenic |
| AAGATTTAAAGAAAATTTGT+GGG | 0.441480 | 8.3:+60184758 | MS.gene37500:CDS |
| ATCACTAACAATTGTGTTCA+CGG | 0.442584 | 8.3:-60194846 | None:intergenic |
| TCAAATGTTTGATCCCAAAC+AGG | 0.447590 | 8.3:-60195199 | None:intergenic |
| CATAGGATTGTTGTTCCACT+TGG | 0.449847 | 8.3:+60194884 | MS.gene37500:CDS |
| ACAAGCTCCTGAGCAAGCTT+TGG | 0.455620 | 8.3:+60191158 | MS.gene37500:CDS |
| CAACTGAACTCACAGGTTCT+GGG | 0.458227 | 8.3:-60169055 | None:intergenic |
| CCATTGTCGGCAAACACTCT+TGG | 0.468148 | 8.3:+60169368 | MS.gene37500:CDS |
| AGTAAAATCCGAGGCTTCCT+GGG | 0.469471 | 8.3:-60169107 | None:intergenic |
| CAAGTTGTTGTTAACAACCT+TGG | 0.472630 | 8.3:-60195173 | None:intergenic |
| TCTTTAAATCTTCTCTGTTG+AGG | 0.472679 | 8.3:-60184747 | None:intergenic |
| TTACAAAGAGGTTAGCTTGT+GGG | 0.472912 | 8.3:+60170761 | MS.gene37500:CDS |
| TCTGCTAAGTAAACCTGGAT+AGG | 0.474530 | 8.3:-60193556 | None:intergenic |
| GTTGGAAGTGGCATTGGCGC+TGG | 0.474839 | 8.3:+60197621 | MS.gene37500:CDS |
| GGCTGCAACAATGGTGATCA+AGG | 0.475012 | 8.3:+60191910 | MS.gene37500:intron |
| CTACCATTTACTCTACTGTA+AGG | 0.476278 | 8.3:+60170815 | MS.gene37500:CDS |
| CCTTCTTATCTTTCACCTTG+AGG | 0.476760 | 8.3:-60196686 | None:intergenic |
| AAACCTCAAGAATGAGTGAC+TGG | 0.477259 | 8.3:-60195246 | None:intergenic |
| TCACATTGAGCTTCGCAAAT+GGG | 0.477326 | 8.3:+60169325 | MS.gene37500:CDS |
| CGAATATACAGAACAACATA+AGG | 0.479749 | 8.3:-60195130 | None:intergenic |
| TCAATCATATCTGAAATTCC+AGG | 0.484752 | 8.3:-60194730 | None:intergenic |
| TAATGGACGTAGATCTCCGC+TGG | 0.485216 | 8.3:+60193479 | MS.gene37500:CDS |
| CACATGTATCGTGCGAGCCT+GGG | 0.485764 | 8.3:+60170621 | MS.gene37500:CDS |
| CTCATCTCCATTGACGAGCT+TGG | 0.486057 | 8.3:+60170718 | MS.gene37500:CDS |
| GGGTCAGGTGTTGGACTAGT+TGG | 0.486254 | 8.3:+60197570 | MS.gene37500:CDS |
| ACAATGGATGCAGTAGGGTC+AGG | 0.488631 | 8.3:+60197555 | MS.gene37500:CDS |
| GGTGCCGGAGCTGGACTTGT+TGG | 0.490540 | 8.3:+60197603 | MS.gene37500:CDS |
| ACAGTAGAGTAAATGGTAGA+AGG | 0.491124 | 8.3:-60170811 | None:intergenic |
| GCTGTTGACATCAAAATCCT+TGG | 0.493910 | 8.3:+60184721 | MS.gene37500:CDS |
| ATGGCTGGTGCAACAAAGAA+TGG | 0.494922 | 8.3:-60170655 | None:intergenic |
| AGAAATACCAAGCTCGTCAA+TGG | 0.495582 | 8.3:-60170725 | None:intergenic |
| GGACTAGTTGGAACTGGCAT+TGG | 0.497335 | 8.3:+60197582 | MS.gene37500:CDS |
| TCGCAAATGGGCTGATATCA+TGG | 0.497396 | 8.3:+60169337 | MS.gene37500:CDS |
| GTGGGGAGAATCTTCCTGAA+TGG | 0.499769 | 8.3:+60184776 | MS.gene37500:CDS |
| CAGGTTTATGATGAAGACAT+TGG | 0.499930 | 8.3:+60196564 | MS.gene37500:intron |
| CTGGAAGAGTACAGACCTCC+TGG | 0.500265 | 8.3:+60191950 | MS.gene37500:CDS |
| AGGTTTATGATGAAGACATT+GGG | 0.500537 | 8.3:+60196565 | MS.gene37500:intron |
| CCAAGAGTGTTTGCCGACAA+TGG | 0.502597 | 8.3:-60169368 | None:intergenic |
| CATGGTCATTGCTCCATTGT+CGG | 0.504477 | 8.3:+60169355 | MS.gene37500:CDS |
| CTGCTAAGTAAACCTGGATA+GGG | 0.504937 | 8.3:-60193555 | None:intergenic |
| GGAGCTGGACTTGTTGGCAG+TGG | 0.507435 | 8.3:+60197642 | MS.gene37500:CDS |
| ATACTCACTTCTGCTACAAA+TGG | 0.507555 | 8.3:-60191181 | None:intergenic |
| CAAATGGCCAAAGCTTGCTC+AGG | 0.509120 | 8.3:-60191165 | None:intergenic |
| ACTTCCAACAAGTCCAGCTC+CGG | 0.509759 | 8.3:-60197607 | None:intergenic |
| GGTGTTGGACTAGTTGGAAC+TGG | 0.510124 | 8.3:+60197576 | MS.gene37500:CDS |
| TGTATACGGACGATGATGAA+TGG | 0.510618 | 8.3:+60169270 | MS.gene37500:CDS |
| CATAGCATTGATGGCAGGAT+GGG | 0.514159 | 8.3:+60181487 | MS.gene37500:CDS |
| GGACTTGTTGGAAGTGGCAT+TGG | 0.514269 | 8.3:+60197615 | MS.gene37500:CDS |
| CTTCTCTGTTGAGGGATCCA+AGG | 0.514720 | 8.3:-60184738 | None:intergenic |
| CACATAAAAGTGTTCATGGC+TGG | 0.518632 | 8.3:-60170670 | None:intergenic |
| GTTGGAACTGGCATTGGTGC+CGG | 0.519477 | 8.3:+60197588 | MS.gene37500:CDS |
| GGTAGCGGACTTAGCAAGGC+AGG | 0.519809 | 8.3:+60197678 | MS.gene37500:CDS |
| CCTCAAGGTGAAAGATAAGA+AGG | 0.519976 | 8.3:+60196686 | MS.gene37500:CDS |
| TACAAAGAGGTTAGCTTGTG+GGG | 0.520289 | 8.3:+60170762 | MS.gene37500:CDS |
| AAGTTCAGCAAGCTGTCACT+TGG | 0.526433 | 8.3:+60191986 | MS.gene37500:CDS |
| CCAGAACCTGTGAGTTCAGT+TGG | 0.528188 | 8.3:+60169056 | MS.gene37500:CDS |
| TGCATGTACCTTTACGGTCA+AGG | 0.529547 | 8.3:-60196718 | None:intergenic |
| ATGAGATGCCGCTCTGTGAA+AGG | 0.531219 | 8.3:-60170700 | None:intergenic |
| CAACAATCCTATGTGGCCAT+TGG | 0.532619 | 8.3:-60194874 | None:intergenic |
| CATTGTGCTACCTATGACGC+CGG | 0.533916 | 8.3:-60197538 | None:intergenic |
| ACGATGATGAATGGTCTAGT+TGG | 0.538549 | 8.3:+60169279 | MS.gene37500:CDS |
| GAATGGCTTGCTATAGTCCC+AGG | 0.538623 | 8.3:-60170638 | None:intergenic |
| AACGTAGCTCCAAAAGTTGA+AGG | 0.539990 | 8.3:+60192010 | MS.gene37500:CDS |
| TGCTGTTGTAGATTGCTGGA+GGG | 0.541864 | 8.3:+60170581 | MS.gene37500:intron |
| GCTTGTGGGGATTATGGCAA+TGG | 0.544428 | 8.3:+60170775 | MS.gene37500:CDS |
| GCATAGCATTGATGGCAGGA+TGG | 0.544883 | 8.3:+60181486 | MS.gene37500:CDS |
| CAAAATCACATCTTTAGGAA+TGG | 0.549135 | 8.3:-60169249 | None:intergenic |
| CTTTGGTAGCGGACTTAGCA+AGG | 0.551100 | 8.3:+60197674 | MS.gene37500:CDS |
| ATTGGGCAAGATAAGCGATT+GGG | 0.551386 | 8.3:+60196582 | MS.gene37500:CDS |
| AGGATTGTTGTTCCACTTGG+CGG | 0.551442 | 8.3:+60194887 | MS.gene37500:CDS |
| TGTACTTCCAATTCAATAAG+GGG | 0.551442 | 8.3:-60196618 | None:intergenic |
| GGATTATGGCAATGGTGCAA+TGG | 0.551856 | 8.3:+60170783 | MS.gene37500:CDS |
| GGTAGCACAATGGATGCAGT+AGG | 0.553013 | 8.3:+60197549 | MS.gene37500:CDS |
| TGAGTTGATTGCAGAAGACA+AGG | 0.554967 | 8.3:+60195219 | MS.gene37500:CDS |
| TTGCTGCTCCCAGGAAGCCT+CGG | 0.556191 | 8.3:+60169099 | MS.gene37500:CDS |
| GACTTGGAGCTTAAACCTCA+TGG | 0.556221 | 8.3:+60195040 | MS.gene37500:CDS |
| AACTCCAGTTCATAATCCAC+AGG | 0.559720 | 8.3:+60197758 | MS.gene37500:CDS |
| GGAGCTGGACTTGTTGGAAG+TGG | 0.560309 | 8.3:+60197609 | MS.gene37500:CDS |
| CAGTGAATATTGCTGCTCCC+AGG | 0.561605 | 8.3:+60169090 | MS.gene37500:CDS |
| ATATGCTCCAATGGCCACAT+AGG | 0.564164 | 8.3:+60194867 | MS.gene37500:CDS |
| GACCCAGTCACTCATTCTTG+AGG | 0.564381 | 8.3:+60195243 | MS.gene37500:CDS |
| CATTGGGCAAGATAAGCGAT+TGG | 0.564623 | 8.3:+60196581 | MS.gene37500:CDS |
| TGAACTCACAGGTTCTGGGC+TGG | 0.571240 | 8.3:-60169051 | None:intergenic |
| TGGTCTAGTTGGAAGAAACT+AGG | 0.573969 | 8.3:+60169290 | MS.gene37500:CDS |
| TTTGGCATAGCATTGATGGC+AGG | 0.576322 | 8.3:+60181482 | MS.gene37500:CDS |
| TGTTGTTGCTCTTCTTGCTG+AGG | 0.577292 | 8.3:+60193738 | MS.gene37500:CDS |
| TCATTCCACCTGTTACAAAG+AGG | 0.579111 | 8.3:+60170749 | MS.gene37500:CDS |
| TTTATTCAAGGTTAAAACCA+AGG | 0.579947 | 8.3:+60195156 | MS.gene37500:CDS |
| AAGGGATCTCTTCAGCAAGT+TGG | 0.580409 | 8.3:-60193702 | None:intergenic |
| GATGCAGTAGGGTCAGGTGT+TGG | 0.583496 | 8.3:+60197561 | MS.gene37500:CDS |
| GATTATACTCTGAAAGCTGT+CGG | 0.584378 | 8.3:+60194688 | MS.gene37500:CDS |
| TCGAGGAACCTTGACCGTAA+AGG | 0.588579 | 8.3:+60196710 | MS.gene37500:CDS |
| GCTAACCTCTTTGTAACAGG+TGG | 0.589007 | 8.3:-60170754 | None:intergenic |
| GTATTACCAATTTAACAAAG+AGG | 0.591199 | 8.3:+60197452 | MS.gene37500:CDS |
| GGATAGGGATTGATGCAACA+AGG | 0.592253 | 8.3:-60193540 | None:intergenic |
| TTCTCTCCAACTGAACTCAC+AGG | 0.597337 | 8.3:-60169062 | None:intergenic |
| TTGTTAGTGATATGCTCCAA+TGG | 0.602275 | 8.3:+60194858 | MS.gene37500:CDS |
| CTTGCTAAGTCCGCTACCAA+AGG | 0.604267 | 8.3:-60197673 | None:intergenic |
| CAGCAAGTTGGAATATAACA+CGG | 0.604979 | 8.3:-60193690 | None:intergenic |
| GTCGGCAAACACTCTTGGCA+AGG | 0.605734 | 8.3:+60169373 | MS.gene37500:CDS |
| TTGCTGTTGTAGATTGCTGG+AGG | 0.607972 | 8.3:+60170580 | MS.gene37500:intron |
| AGGAAGAAACTGAAAGCCGC+CGG | 0.609628 | 8.3:+60197519 | MS.gene37500:CDS |
| CAGGTCATAGTGGTTCAAGA+AGG | 0.610308 | 8.3:+60197724 | MS.gene37500:CDS |
| AATGGACGTAGATCTCCGCT+GGG | 0.611373 | 8.3:+60193480 | MS.gene37500:CDS |
| GGTCTGTACTCTTCCAGAAG+AGG | 0.614089 | 8.3:-60191944 | None:intergenic |
| GACGTAGATCTCCGCTGGGG+TGG | 0.614726 | 8.3:+60193484 | MS.gene37500:CDS |
| AGGACCATAACAGGTCATAG+TGG | 0.621920 | 8.3:+60197714 | MS.gene37500:CDS |
| AGATTTAAAGAAAATTTGTG+GGG | 0.624990 | 8.3:+60184759 | MS.gene37500:CDS |
| TATGAGTCAAAGGCTCAGCA+AGG | 0.625267 | 8.3:+60170844 | MS.gene37500:CDS |
| AACCTCAAGAATGAGTGACT+GGG | 0.625333 | 8.3:-60195245 | None:intergenic |
| CAGGATGGGCTCGTATGATG+AGG | 0.626744 | 8.3:+60181501 | MS.gene37500:CDS |
| ACAACAACAGCAGAAATGCA+AGG | 0.628716 | 8.3:-60193721 | None:intergenic |
| AAGTGCCAAGAGAATTGCAA+AGG | 0.631578 | 8.3:+60181529 | MS.gene37500:CDS |
| TACTAGTGGAAGTGTTGCTG+CGG | 0.634094 | 8.3:+60169133 | MS.gene37500:CDS |
| AGCAAGTTGGAATATAACAC+GGG | 0.634787 | 8.3:-60193689 | None:intergenic |
| GTGAAAGATAAGAAGGATCG+AGG | 0.635254 | 8.3:+60196693 | MS.gene37500:CDS |
| CATAGTGGTTCAAGAAGGAG+TGG | 0.636026 | 8.3:+60197729 | MS.gene37500:CDS |
| TATACTCTGAAAGCTGTCGG+TGG | 0.639325 | 8.3:+60194691 | MS.gene37500:CDS |
| AGGCAGGAAAGTTTATGGGA+AGG | 0.640868 | 8.3:+60197694 | MS.gene37500:CDS |
| CAACAACAGCAGAAATGCAA+GGG | 0.643698 | 8.3:-60193720 | None:intergenic |
| GTTACAAAGAGGTTAGCTTG+TGG | 0.645490 | 8.3:+60170760 | MS.gene37500:CDS |
| GTCATCGCTTGATACCCTCA+AGG | 0.648469 | 8.3:+60196671 | MS.gene37500:CDS |
| GATAGGGATTGATGCAACAA+GGG | 0.649326 | 8.3:-60193539 | None:intergenic |
| GTAGCACAATGGATGCAGTA+GGG | 0.650123 | 8.3:+60197550 | MS.gene37500:CDS |
| TGACCATTAGGCTGCAACAA+TGG | 0.651157 | 8.3:+60191901 | MS.gene37500:intron |
| AGTGGAACAACAATCCTATG+TGG | 0.660298 | 8.3:-60194881 | None:intergenic |
| TCGACAGCCAAAATAATACA+GGG | 0.688231 | 8.3:-60193511 | None:intergenic |
| CGTGTGCAGAGTCTTACCAA+AGG | 0.692568 | 8.3:+60193448 | MS.gene37500:CDS |
| GTCACTTTAAGGTTTCCATG+AGG | 0.693888 | 8.3:-60195055 | None:intergenic |
| CTAGTAGCGAGTAAAATCCG+AGG | 0.704126 | 8.3:-60169116 | None:intergenic |
| TCAACAGGGATACCGCCAAG+TGG | 0.705197 | 8.3:-60194899 | None:intergenic |
| TTCAATGAAGTAATTCCAGG+AGG | 0.708678 | 8.3:-60191965 | None:intergenic |
| CGGCGTCATAGGTAGCACAA+TGG | 0.721145 | 8.3:+60197539 | MS.gene37500:CDS |
| ATATTTACCTAATATCAACA+GGG | 0.721909 | 8.3:-60194913 | None:intergenic |
| TACAGGGATCACCACCCCAG+CGG | 0.723983 | 8.3:-60193495 | None:intergenic |
| GGAACAATCCTTTCACAGAG+CGG | 0.724970 | 8.3:+60170692 | MS.gene37500:CDS |
| TGTGCTACCTATGACGCCGG+CGG | 0.725015 | 8.3:-60197535 | None:intergenic |
| ATGGACGTAGATCTCCGCTG+GGG | 0.777081 | 8.3:+60193481 | MS.gene37500:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 60169047 | 60197809 | 60169047 | ID=MS.gene37500 |
| chr8.3 | mRNA | 60169047 | 60197809 | 60169047 | ID=MS.gene37500.t1;Parent=MS.gene37500 |
| chr8.3 | exon | 60169047 | 60169394 | 60169047 | ID=MS.gene37500.t1.exon1;Parent=MS.gene37500.t1 |
| chr8.3 | CDS | 60169047 | 60169394 | 60169047 | ID=cds.MS.gene37500.t1;Parent=MS.gene37500.t1 |
| chr8.3 | exon | 60170592 | 60170865 | 60170592 | ID=MS.gene37500.t1.exon2;Parent=MS.gene37500.t1 |
| chr8.3 | CDS | 60170592 | 60170865 | 60170592 | ID=cds.MS.gene37500.t1;Parent=MS.gene37500.t1 |
| chr8.3 | exon | 60181417 | 60181550 | 60181417 | ID=MS.gene37500.t1.exon3;Parent=MS.gene37500.t1 |
| chr8.3 | CDS | 60181417 | 60181550 | 60181417 | ID=cds.MS.gene37500.t1;Parent=MS.gene37500.t1 |
| chr8.3 | exon | 60184721 | 60184822 | 60184721 | ID=MS.gene37500.t1.exon4;Parent=MS.gene37500.t1 |
| chr8.3 | CDS | 60184721 | 60184822 | 60184721 | ID=cds.MS.gene37500.t1;Parent=MS.gene37500.t1 |
| chr8.3 | exon | 60191145 | 60191195 | 60191145 | ID=MS.gene37500.t1.exon5;Parent=MS.gene37500.t1 |
| chr8.3 | CDS | 60191145 | 60191195 | 60191145 | ID=cds.MS.gene37500.t1;Parent=MS.gene37500.t1 |
| chr8.3 | exon | 60191911 | 60192031 | 60191911 | ID=MS.gene37500.t1.exon6;Parent=MS.gene37500.t1 |
| chr8.3 | CDS | 60191911 | 60192031 | 60191911 | ID=cds.MS.gene37500.t1;Parent=MS.gene37500.t1 |
| chr8.3 | exon | 60193443 | 60193564 | 60193443 | ID=MS.gene37500.t1.exon7;Parent=MS.gene37500.t1 |
| chr8.3 | CDS | 60193443 | 60193564 | 60193443 | ID=cds.MS.gene37500.t1;Parent=MS.gene37500.t1 |
| chr8.3 | exon | 60193661 | 60193759 | 60193661 | ID=MS.gene37500.t1.exon8;Parent=MS.gene37500.t1 |
| chr8.3 | CDS | 60193661 | 60193759 | 60193661 | ID=cds.MS.gene37500.t1;Parent=MS.gene37500.t1 |
| chr8.3 | exon | 60194673 | 60194753 | 60194673 | ID=MS.gene37500.t1.exon9;Parent=MS.gene37500.t1 |
| chr8.3 | CDS | 60194673 | 60194753 | 60194673 | ID=cds.MS.gene37500.t1;Parent=MS.gene37500.t1 |
| chr8.3 | exon | 60194842 | 60194927 | 60194842 | ID=MS.gene37500.t1.exon10;Parent=MS.gene37500.t1 |
| chr8.3 | CDS | 60194842 | 60194927 | 60194842 | ID=cds.MS.gene37500.t1;Parent=MS.gene37500.t1 |
| chr8.3 | exon | 60195039 | 60195264 | 60195039 | ID=MS.gene37500.t1.exon11;Parent=MS.gene37500.t1 |
| chr8.3 | CDS | 60195039 | 60195264 | 60195039 | ID=cds.MS.gene37500.t1;Parent=MS.gene37500.t1 |
| chr8.3 | exon | 60196567 | 60196731 | 60196567 | ID=MS.gene37500.t1.exon12;Parent=MS.gene37500.t1 |
| chr8.3 | CDS | 60196567 | 60196731 | 60196567 | ID=cds.MS.gene37500.t1;Parent=MS.gene37500.t1 |
| chr8.3 | exon | 60197447 | 60197809 | 60197447 | ID=MS.gene37500.t1.exon13;Parent=MS.gene37500.t1 |
| chr8.3 | CDS | 60197447 | 60197809 | 60197447 | ID=cds.MS.gene37500.t1;Parent=MS.gene37500.t1 |
| Gene Sequence |
| Protein sequence |
