Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050073.t1 | XP_003615175.1 | 94 | 469 | 26 | 1 | 1 | 469 | 1 | 467 | 5.90E-249 | 869.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050073.t1 | Q9LHN7 | 73.9 | 436 | 112 | 1 | 30 | 465 | 33 | 466 | 1.1e-187 | 657.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050073.t1 | G7KAH9 | 94.0 | 469 | 26 | 1 | 1 | 469 | 1 | 467 | 4.2e-249 | 869.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene050073 | 0.813134 | 2.99E-51 | -1.69E-46 |
| MS.gene049435 | MS.gene050073 | 0.818317 | 2.09E-52 | -1.69E-46 |
| MS.gene050073 | MS.gene051279 | 0.825339 | 4.95E-54 | -1.69E-46 |
| MS.gene050073 | MS.gene05415 | 0.800214 | 1.60E-48 | -1.69E-46 |
| MS.gene050073 | MS.gene054236 | 0.806769 | 7.00E-50 | -1.69E-46 |
| MS.gene050073 | MS.gene054651 | 0.802561 | 5.29E-49 | -1.69E-46 |
| MS.gene050073 | MS.gene055227 | 0.805851 | 1.09E-49 | -1.69E-46 |
| MS.gene050073 | MS.gene056174 | 0.828477 | 8.80E-55 | -1.69E-46 |
| MS.gene050073 | MS.gene057208 | 0.812053 | 5.14E-51 | -1.69E-46 |
| MS.gene050073 | MS.gene057869 | 0.813572 | 2.39E-51 | -1.69E-46 |
| MS.gene050073 | MS.gene058010 | 0.801247 | 9.85E-49 | -1.69E-46 |
| MS.gene050073 | MS.gene058979 | 0.801802 | 7.58E-49 | -1.69E-46 |
| MS.gene050073 | MS.gene06269 | 0.817765 | 2.78E-52 | -1.69E-46 |
| MS.gene050073 | MS.gene063407 | 0.805665 | 1.20E-49 | -1.69E-46 |
| MS.gene050073 | MS.gene065904 | 0.805556 | 1.26E-49 | -1.69E-46 |
| MS.gene050073 | MS.gene066320 | 0.804506 | 2.09E-49 | -1.69E-46 |
| MS.gene050073 | MS.gene066335 | 0.807651 | 4.56E-50 | -1.69E-46 |
| MS.gene050073 | MS.gene06643 | 0.805248 | 1.46E-49 | -1.69E-46 |
| MS.gene050073 | MS.gene067173 | 0.818731 | 1.68E-52 | -1.69E-46 |
| MS.gene050073 | MS.gene06723 | 0.821379 | 4.17E-53 | -1.69E-46 |
| MS.gene050073 | MS.gene067410 | 0.826908 | 2.10E-54 | -1.69E-46 |
| MS.gene050073 | MS.gene068526 | 0.811705 | 6.12E-51 | -1.69E-46 |
| MS.gene050073 | MS.gene06857 | 0.805648 | 1.21E-49 | -1.69E-46 |
| MS.gene050073 | MS.gene068924 | 0.807819 | 4.20E-50 | -1.69E-46 |
| MS.gene050073 | MS.gene070301 | 0.814937 | 1.19E-51 | -1.69E-46 |
| MS.gene050073 | MS.gene070478 | 0.827024 | 1.97E-54 | -1.69E-46 |
| MS.gene050073 | MS.gene073139 | 0.811983 | 5.33E-51 | -1.69E-46 |
| MS.gene050073 | MS.gene073727 | 0.817879 | 2.62E-52 | -1.69E-46 |
| MS.gene050073 | MS.gene073853 | 0.810101 | 1.36E-50 | -1.69E-46 |
| MS.gene050073 | MS.gene074604 | 0.800255 | 1.57E-48 | -1.69E-46 |
| MS.gene050073 | MS.gene21927 | 0.804427 | 2.17E-49 | -1.69E-46 |
| MS.gene050073 | MS.gene21929 | 0.812229 | 4.71E-51 | -1.69E-46 |
| MS.gene050073 | MS.gene22271 | 0.805958 | 1.04E-49 | -1.69E-46 |
| MS.gene050073 | MS.gene23030 | 0.808191 | 3.50E-50 | -1.69E-46 |
| MS.gene050073 | MS.gene24067 | 0.820949 | 5.23E-53 | -1.69E-46 |
| MS.gene050073 | MS.gene25151 | 0.802922 | 4.46E-49 | -1.69E-46 |
| MS.gene050073 | MS.gene27054 | 0.808642 | 2.80E-50 | -1.69E-46 |
| MS.gene050073 | MS.gene30301 | 0.81517 | 1.06E-51 | -1.69E-46 |
| MS.gene050073 | MS.gene31142 | 0.807375 | 5.21E-50 | -1.69E-46 |
| MS.gene050073 | MS.gene31283 | 0.820001 | 8.64E-53 | -1.69E-46 |
| MS.gene050073 | MS.gene32747 | 0.800977 | 1.12E-48 | -1.69E-46 |
| MS.gene050073 | MS.gene32976 | 0.810847 | 9.40E-51 | -1.69E-46 |
| MS.gene050073 | MS.gene35107 | 0.815265 | 1.01E-51 | -1.69E-46 |
| MS.gene050073 | MS.gene36440 | 0.804416 | 2.18E-49 | -1.69E-46 |
| MS.gene050073 | MS.gene37086 | 0.800041 | 1.74E-48 | -1.69E-46 |
| MS.gene050073 | MS.gene37500 | 0.805723 | 1.16E-49 | -1.69E-46 |
| MS.gene050073 | MS.gene37612 | 0.820409 | 6.97E-53 | -1.69E-46 |
| MS.gene050073 | MS.gene41990 | 0.821215 | 4.55E-53 | -1.69E-46 |
| MS.gene050073 | MS.gene42645 | 0.81828 | 2.13E-52 | -1.69E-46 |
| MS.gene050073 | MS.gene43430 | 0.809851 | 1.54E-50 | -1.69E-46 |
| MS.gene050073 | MS.gene45422 | 0.80086 | 1.18E-48 | -1.69E-46 |
| MS.gene050073 | MS.gene45507 | 0.804719 | 1.89E-49 | -1.69E-46 |
| MS.gene050073 | MS.gene53064 | 0.813786 | 2.15E-51 | -1.69E-46 |
| MS.gene050073 | MS.gene53476 | 0.801358 | 9.35E-49 | -1.69E-46 |
| MS.gene050073 | MS.gene54026 | 0.816007 | 6.90E-52 | -1.69E-46 |
| MS.gene050073 | MS.gene58686 | 0.800347 | 1.50E-48 | -1.69E-46 |
| MS.gene050073 | MS.gene58919 | 0.806559 | 7.76E-50 | -1.69E-46 |
| MS.gene050073 | MS.gene60566 | 0.810873 | 9.28E-51 | -1.69E-46 |
| MS.gene050073 | MS.gene60680 | 0.801768 | 7.70E-49 | -1.69E-46 |
| MS.gene050073 | MS.gene60827 | 0.810675 | 1.02E-50 | -1.69E-46 |
| MS.gene050073 | MS.gene62250 | 0.81699 | 4.16E-52 | -1.69E-46 |
| MS.gene050073 | MS.gene63030 | 0.815116 | 1.09E-51 | -1.69E-46 |
| MS.gene050073 | MS.gene64143 | 0.827406 | 1.59E-54 | -1.69E-46 |
| MS.gene050073 | MS.gene64772 | 0.805212 | 1.49E-49 | -1.69E-46 |
| MS.gene050073 | MS.gene67662 | 0.816502 | 5.35E-52 | -1.69E-46 |
| MS.gene050073 | MS.gene67893 | 0.809838 | 1.55E-50 | -1.69E-46 |
| MS.gene050073 | MS.gene67930 | 0.806231 | 9.10E-50 | -1.69E-46 |
| MS.gene050073 | MS.gene68999 | 0.800494 | 1.40E-48 | -1.69E-46 |
| MS.gene050073 | MS.gene71597 | 0.815289 | 9.98E-52 | -1.69E-46 |
| MS.gene050073 | MS.gene72400 | 0.809326 | 2.00E-50 | -1.69E-46 |
| MS.gene050073 | MS.gene75184 | 0.81313 | 2.99E-51 | -1.69E-46 |
| MS.gene050073 | MS.gene79285 | 0.81691 | 4.33E-52 | -1.69E-46 |
| MS.gene050073 | MS.gene81047 | 0.832767 | 7.83E-56 | -1.69E-46 |
| MS.gene050073 | MS.gene83538 | 0.830717 | 2.51E-55 | -1.69E-46 |
| MS.gene050073 | MS.gene84865 | 0.805112 | 1.56E-49 | -1.69E-46 |
| MS.gene050073 | MS.gene86036 | 0.804298 | 2.31E-49 | -1.69E-46 |
| MS.gene050073 | MS.gene89344 | 0.828759 | 7.52E-55 | -1.69E-46 |
| MS.gene050073 | MS.gene90585 | 0.800203 | 1.61E-48 | -1.69E-46 |
| MS.gene050073 | MS.gene96511 | -0.802354 | 5.84E-49 | -1.69E-46 |
| MS.gene050073 | MS.gene97534 | 0.802345 | 5.86E-49 | -1.69E-46 |
| MS.gene050073 | MS.gene99746 | 0.801527 | 8.63E-49 | -1.69E-46 |
| MS.gene050073 | MS.gene99858 | 0.803825 | 2.90E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene050073 | MS.gene56252 | PPI |
| MS.gene050073 | MS.gene51623 | PPI |
| MS.gene050073 | MS.gene84379 | PPI |
| MS.gene050073 | MS.gene009450 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050073.t1 | MTR_5g064750 | 97.015 | 469 | 12 | 2 | 1 | 469 | 1 | 467 | 0.0 | 900 |
| MS.gene050073.t1 | MTR_8g085630 | 63.801 | 442 | 160 | 0 | 19 | 460 | 32 | 473 | 0.0 | 587 |
| MS.gene050073.t1 | MTR_1g112950 | 47.380 | 458 | 238 | 2 | 5 | 462 | 4 | 458 | 3.78e-146 | 426 |
| MS.gene050073.t1 | MTR_5g026780 | 46.847 | 444 | 234 | 2 | 19 | 462 | 22 | 463 | 4.83e-137 | 403 |
| MS.gene050073.t1 | MTR_5g026780 | 46.847 | 444 | 234 | 2 | 19 | 462 | 38 | 479 | 5.54e-137 | 403 |
| MS.gene050073.t1 | MTR_0031s0060 | 44.912 | 452 | 216 | 4 | 11 | 462 | 130 | 548 | 3.79e-130 | 392 |
| MS.gene050073.t1 | MTR_5g005160 | 43.750 | 448 | 248 | 3 | 18 | 464 | 1 | 445 | 3.45e-123 | 368 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050073.t1 | AT3G13620 | 72.807 | 456 | 122 | 1 | 13 | 468 | 16 | 469 | 0.0 | 683 |
| MS.gene050073.t1 | AT1G31830 | 47.991 | 448 | 224 | 5 | 19 | 462 | 23 | 465 | 1.14e-142 | 417 |
| MS.gene050073.t1 | AT1G31830 | 47.991 | 448 | 224 | 5 | 19 | 462 | 39 | 481 | 1.88e-142 | 417 |
| MS.gene050073.t1 | AT3G19553 | 47.390 | 479 | 229 | 6 | 1 | 464 | 1 | 471 | 4.34e-142 | 416 |
| MS.gene050073.t1 | AT5G05630 | 45.514 | 457 | 246 | 2 | 5 | 459 | 29 | 484 | 1.15e-138 | 407 |
| MS.gene050073.t1 | AT1G31820 | 47.392 | 441 | 225 | 4 | 21 | 459 | 30 | 465 | 3.21e-132 | 391 |
Find 94 sgRNAs with CRISPR-Local
Find 141 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATAAGCACTACTACTTAAT+TGG | 0.091375 | 5.4:+50467167 | None:intergenic |
| CTGGAAAATGGTTGAAAATT+TGG | 0.098359 | 5.4:-50467234 | MS.gene050073:CDS |
| TACACCTTGGTTGGGCATTT+TGG | 0.165003 | 5.4:-50467077 | MS.gene050073:CDS |
| AACAATTGCTGTTTCTTATA+TGG | 0.175964 | 5.4:-50467041 | MS.gene050073:CDS |
| CCCATTAAAGAGCCTGAAAA+TGG | 0.209394 | 5.4:+50467787 | None:intergenic |
| CATTTATGTGGATTTATCTT+AGG | 0.215603 | 5.4:+50467523 | None:intergenic |
| TCAGCAGTTATTAGAGCTTC+TGG | 0.230357 | 5.4:+50467871 | None:intergenic |
| GTATAGTTTGGGTATGATAT+TGG | 0.268899 | 5.4:-50466984 | MS.gene050073:CDS |
| TCCTTATCTTCCCTTTCATT+TGG | 0.274826 | 5.4:-50467900 | MS.gene050073:CDS |
| CTAAGATAAATCCACATAAA+TGG | 0.281292 | 5.4:-50467522 | MS.gene050073:CDS |
| CTGAGATTATTGCTGGAAAA+TGG | 0.284737 | 5.4:-50467246 | MS.gene050073:CDS |
| TAGTAGTGCTTATCAAGTTT+TGG | 0.284965 | 5.4:-50467158 | MS.gene050073:CDS |
| TTGAAAATTTGGGTTGAAAT+TGG | 0.294482 | 5.4:-50467223 | MS.gene050073:CDS |
| TGAGGAATATGAGAGGGATT+AGG | 0.299565 | 5.4:+50468004 | None:intergenic |
| AAATTCATTGGAATCTTATA+AGG | 0.308786 | 5.4:+50466911 | None:intergenic |
| GAGGAATATGAGAGGGATTA+GGG | 0.310047 | 5.4:+50468005 | None:intergenic |
| CCACATAAATGGATTAGTTT+AGG | 0.314833 | 5.4:-50467511 | MS.gene050073:CDS |
| GGCCCTCTCTTTGCACTTCT+CGG | 0.317963 | 5.4:-50467925 | MS.gene050073:CDS |
| TGGAAAATGGTTGAAAATTT+GGG | 0.326002 | 5.4:-50467233 | MS.gene050073:CDS |
| GTGAGTGGTTTGATGACTTT+AGG | 0.338550 | 5.4:-50466806 | MS.gene050073:CDS |
| ATGGATTAGTTTAGGTCAAA+AGG | 0.342823 | 5.4:-50467503 | MS.gene050073:CDS |
| TTTGGGAATGGTGGTGGTGG+TGG | 0.349220 | 5.4:+50468050 | None:intergenic |
| AATCAAAGTGAATGGGAAAC+TGG | 0.353220 | 5.4:-50467286 | MS.gene050073:CDS |
| ATAACAAAACCACCGTTTCC+TGG | 0.365386 | 5.4:+50467832 | None:intergenic |
| AATTGTGTAGGATGAAGAAT+TGG | 0.368991 | 5.4:-50466757 | MS.gene050073:CDS |
| TTTGTGGAATTAGGTCAAAA+TGG | 0.373378 | 5.4:-50467105 | MS.gene050073:CDS |
| GACCTAATTCCACAAAACTT+TGG | 0.398820 | 5.4:+50467112 | None:intergenic |
| ATAAGCACTACTACTTAATT+GGG | 0.402363 | 5.4:+50467168 | None:intergenic |
| GATTAGGGTTAGTTTCTTTG+TGG | 0.402905 | 5.4:+50468020 | None:intergenic |
| TATACTGGTTTAACAATTGT+TGG | 0.403572 | 5.4:-50467613 | MS.gene050073:CDS |
| AAAAGGGTGTTAAGAAAGAT+TGG | 0.406094 | 5.4:-50467486 | MS.gene050073:CDS |
| GGGCCGGCGGCTTGAACGGC+GGG | 0.407242 | 5.4:+50467943 | None:intergenic |
| CAAGCTGCTGAGATTATTGC+TGG | 0.414273 | 5.4:-50467253 | MS.gene050073:CDS |
| ATATAATCGATGCAGAGAAC+AGG | 0.424951 | 5.4:+50467721 | None:intergenic |
| AGGGCCGGCGGCTTGAACGG+CGG | 0.426646 | 5.4:+50467942 | None:intergenic |
| ACCACCGTTTCCTGGATAAA+CGG | 0.427767 | 5.4:+50467840 | None:intergenic |
| CAGGCTCTTTAATGGGTACA+TGG | 0.427973 | 5.4:-50467780 | MS.gene050073:CDS |
| TGGATTAGTTTAGGTCAAAA+GGG | 0.430138 | 5.4:-50467502 | MS.gene050073:CDS |
| GATAATGTTAGTACTTTAGC+TGG | 0.431843 | 5.4:-50467421 | MS.gene050073:CDS |
| ATTAAAGAGGATGAAGAAGA+TGG | 0.432819 | 5.4:-50466722 | MS.gene050073:CDS |
| GGTGGTGGTGGTTGGAGCGG+TGG | 0.435676 | 5.4:+50468068 | None:intergenic |
| TATGTTGGTGGTGATGTGTT+TGG | 0.437368 | 5.4:-50466885 | MS.gene050073:CDS |
| ACGGCGGGTTCTTCGCCGTA+TGG | 0.438043 | 5.4:+50467958 | None:intergenic |
| TAGGGTTAGTTTCTTTGTGG+TGG | 0.446224 | 5.4:+50468023 | None:intergenic |
| ATGGTTTAATACACCTTGGT+TGG | 0.446678 | 5.4:-50467086 | MS.gene050073:CDS |
| TGGTTTAATACACCTTGGTT+GGG | 0.448792 | 5.4:-50467085 | MS.gene050073:CDS |
| CGGCGGGTTCTTCGCCGTAT+GGG | 0.457464 | 5.4:+50467959 | None:intergenic |
| TTGATGACTTTAGGTGGGAT+TGG | 0.459288 | 5.4:-50466797 | MS.gene050073:CDS |
| ACCAACATAGGCAAATTCAT+TGG | 0.461925 | 5.4:+50466899 | None:intergenic |
| GTGATGTGTTTGGTACCATC+TGG | 0.469712 | 5.4:-50466875 | MS.gene050073:CDS |
| CCTAAACTAATCCATTTATG+TGG | 0.478543 | 5.4:+50467511 | None:intergenic |
| ATAAGAGACATTAAAACAAA+TGG | 0.479214 | 5.4:+50467553 | None:intergenic |
| TTCTTCGCCGTATGGGCCGC+CGG | 0.479835 | 5.4:+50467966 | None:intergenic |
| GAATTAACCACCGTTTATCC+AGG | 0.481954 | 5.4:-50467850 | MS.gene050073:CDS |
| GAGAGGGCCGGCGGCTTGAA+CGG | 0.488019 | 5.4:+50467939 | None:intergenic |
| TGGTGGTTGGAGCGGTGGAA+AGG | 0.490716 | 5.4:+50468073 | None:intergenic |
| TCCAATGAATTTGCCTATGT+TGG | 0.491241 | 5.4:-50466900 | MS.gene050073:CDS |
| TGAATGTGAATCAAAGTGAA+TGG | 0.492330 | 5.4:-50467294 | MS.gene050073:CDS |
| TCCAAATGAAAGGGAAGATA+AGG | 0.504290 | 5.4:+50467899 | None:intergenic |
| GGTGAAGTTTGAGATTAAAG+AGG | 0.504848 | 5.4:-50466735 | MS.gene050073:CDS |
| GAAGTAGCCGGCGGCCCATA+CGG | 0.505411 | 5.4:-50467973 | MS.gene050073:CDS |
| TGAAGAAGATGGGAATATAG+TGG | 0.508863 | 5.4:-50466711 | MS.gene050073:CDS |
| CCATTAAAGAGCCTGAAAAT+GGG | 0.509099 | 5.4:+50467788 | None:intergenic |
| AGTGGTTTGATGACTTTAGG+TGG | 0.509956 | 5.4:-50466803 | MS.gene050073:CDS |
| AATGAATTTGCCTATGTTGG+TGG | 0.511920 | 5.4:-50466897 | MS.gene050073:CDS |
| CAAAATGGTTTAATACACCT+TGG | 0.521677 | 5.4:-50467090 | MS.gene050073:CDS |
| CTGGAATGCTCCAAATGAAA+GGG | 0.522384 | 5.4:+50467890 | None:intergenic |
| AAACACATCACCACCAACAT+AGG | 0.527049 | 5.4:+50466887 | None:intergenic |
| GTGGTTTGATGACTTTAGGT+GGG | 0.528330 | 5.4:-50466802 | MS.gene050073:CDS |
| TTAAAGAGGATGAAGAAGAT+GGG | 0.529578 | 5.4:-50466721 | MS.gene050073:CDS |
| TTATAAGGTCTCACTAACAT+TGG | 0.534080 | 5.4:+50466926 | None:intergenic |
| GAACCCGCCGTTCAAGCCGC+CGG | 0.542038 | 5.4:-50467946 | MS.gene050073:CDS |
| TCTGGAATGCTCCAAATGAA+AGG | 0.543914 | 5.4:+50467889 | None:intergenic |
| ATCAAAGTGAATGGGAAACT+GGG | 0.545968 | 5.4:-50467285 | MS.gene050073:CDS |
| ACCACCGTTTATCCAGGAAA+CGG | 0.548188 | 5.4:-50467844 | MS.gene050073:CDS |
| GGTACATGGAAATTCCTTAG+TGG | 0.551806 | 5.4:-50467766 | MS.gene050073:CDS |
| GAGAAGTGCAAAGAGAGGGC+CGG | 0.553396 | 5.4:+50467927 | None:intergenic |
| GAATGTGAATCAAAGTGAAT+GGG | 0.554110 | 5.4:-50467293 | MS.gene050073:CDS |
| AGCCGAGAAGTGCAAAGAGA+GGG | 0.558173 | 5.4:+50467923 | None:intergenic |
| CTATATTAATGACACCACTA+AGG | 0.563126 | 5.4:+50467752 | None:intergenic |
| ATTGTGTAGGATGAAGAATT+GGG | 0.588960 | 5.4:-50466756 | MS.gene050073:CDS |
| ACAGCAATTAAAAGAGCCAA+AGG | 0.600069 | 5.4:+50467370 | None:intergenic |
| AAGCCGAGAAGTGCAAAGAG+AGG | 0.611962 | 5.4:+50467922 | None:intergenic |
| AAATAGATGAGGAATATGAG+AGG | 0.613347 | 5.4:+50467997 | None:intergenic |
| AATAGATGAGGAATATGAGA+GGG | 0.616828 | 5.4:+50467998 | None:intergenic |
| TATAATCGATGCAGAGAACA+GGG | 0.617544 | 5.4:+50467722 | None:intergenic |
| AAGTGCAAAGAGAGGGCCGG+CGG | 0.633960 | 5.4:+50467930 | None:intergenic |
| CGGCTACTTCAAAATAGATG+AGG | 0.639131 | 5.4:+50467986 | None:intergenic |
| ACCGTTTCCTGGATAAACGG+TGG | 0.655312 | 5.4:+50467843 | None:intergenic |
| ATGAATACAAGAAAACCAGA+TGG | 0.659413 | 5.4:+50466860 | None:intergenic |
| ACCGTTTATCCAGGAAACGG+TGG | 0.661303 | 5.4:-50467841 | MS.gene050073:CDS |
| GATACCAAAATGCCCAACCA+AGG | 0.663483 | 5.4:+50467073 | None:intergenic |
| AAAATTCAAGTTCCAAAACA+GGG | 0.673667 | 5.4:+50467447 | None:intergenic |
| AAGAGAATTGCTATGTAACG+AGG | 0.693948 | 5.4:+50467664 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCTTTTTTATAAAATTGTGT+AGG | - | chr5.4:50468018-50468037 | MS.gene050073:CDS | 15.0% |
| !! | AAATTCATTGGAATCTTATA+AGG | + | chr5.4:50467879-50467898 | None:intergenic | 20.0% |
| !! | ATAAGAGACATTAAAACAAA+TGG | + | chr5.4:50467237-50467256 | None:intergenic | 20.0% |
| !!! | AGCTAATTTTTTGTATAGTT+TGG | - | chr5.4:50467791-50467810 | MS.gene050073:CDS | 20.0% |
| !!! | GCTAATTTTTTGTATAGTTT+GGG | - | chr5.4:50467792-50467811 | MS.gene050073:CDS | 20.0% |
| !!! | TTGTCTTTTCTTAATTATAC+TGG | - | chr5.4:50467159-50467178 | MS.gene050073:CDS | 20.0% |
| ! | ATAAGCACTACTACTTAATT+GGG | + | chr5.4:50467622-50467641 | None:intergenic | 25.0% |
| ! | CATTTATGTGGATTTATCTT+AGG | + | chr5.4:50467267-50467286 | None:intergenic | 25.0% |
| ! | CTAAGATAAATCCACATAAA+TGG | - | chr5.4:50467265-50467284 | MS.gene050073:CDS | 25.0% |
| ! | TGGAAAATGGTTGAAAATTT+GGG | - | chr5.4:50467554-50467573 | MS.gene050073:CDS | 25.0% |
| !! | AAAAATTCAAGTTCCAAAAC+AGG | + | chr5.4:50467344-50467363 | None:intergenic | 25.0% |
| !! | AAAATTCAAGTTCCAAAACA+GGG | + | chr5.4:50467343-50467362 | None:intergenic | 25.0% |
| !! | TGGTTTTCTTGTATTCATAA+TGG | - | chr5.4:50467932-50467951 | MS.gene050073:CDS | 25.0% |
| !!! | AACAATTGCTGTTTCTTATA+TGG | - | chr5.4:50467746-50467765 | MS.gene050073:CDS | 25.0% |
| !!! | GTTTTGGAACTTGAATTTTT+GGG | - | chr5.4:50467344-50467363 | MS.gene050073:CDS | 25.0% |
| !!! | TATACTGGTTTAACAATTGT+TGG | - | chr5.4:50467174-50467193 | MS.gene050073:CDS | 25.0% |
| !!! | TGTTTTGGAACTTGAATTTT+TGG | - | chr5.4:50467343-50467362 | MS.gene050073:CDS | 25.0% |
| !!! | TTGAAAATTTGGGTTGAAAT+TGG | - | chr5.4:50467564-50467583 | MS.gene050073:CDS | 25.0% |
| AAATAGATGAGGAATATGAG+AGG | + | chr5.4:50466793-50466812 | None:intergenic | 30.0% | |
| AATAGATGAGGAATATGAGA+GGG | + | chr5.4:50466792-50466811 | None:intergenic | 30.0% | |
| AATTGTGTAGGATGAAGAAT+TGG | - | chr5.4:50468030-50468049 | MS.gene050073:CDS | 30.0% | |
| ATGAATACAAGAAAACCAGA+TGG | + | chr5.4:50467930-50467949 | None:intergenic | 30.0% | |
| ATTAAAGAGGATGAAGAAGA+TGG | - | chr5.4:50468065-50468084 | MS.gene050073:CDS | 30.0% | |
| ATTGTGTAGGATGAAGAATT+GGG | - | chr5.4:50468031-50468050 | MS.gene050073:CDS | 30.0% | |
| CAAAATGGTTTAATACACCT+TGG | - | chr5.4:50467697-50467716 | MS.gene050073:CDS | 30.0% | |
| CCTAAACTAATCCATTTATG+TGG | + | chr5.4:50467279-50467298 | None:intergenic | 30.0% | |
| CTATATTAATGACACCACTA+AGG | + | chr5.4:50467038-50467057 | None:intergenic | 30.0% | |
| CTGGAAAATGGTTGAAAATT+TGG | - | chr5.4:50467553-50467572 | MS.gene050073:CDS | 30.0% | |
| GAATGTGAATCAAAGTGAAT+GGG | - | chr5.4:50467494-50467513 | MS.gene050073:CDS | 30.0% | |
| GATAAGCACTACTACTTAAT+TGG | + | chr5.4:50467623-50467642 | None:intergenic | 30.0% | |
| GATAATGTTAGTACTTTAGC+TGG | - | chr5.4:50467366-50467385 | MS.gene050073:CDS | 30.0% | |
| GTATAGTTTGGGTATGATAT+TGG | - | chr5.4:50467803-50467822 | MS.gene050073:CDS | 30.0% | |
| TGAATGTGAATCAAAGTGAA+TGG | - | chr5.4:50467493-50467512 | MS.gene050073:CDS | 30.0% | |
| TTAAAGAGGATGAAGAAGAT+GGG | - | chr5.4:50468066-50468085 | MS.gene050073:CDS | 30.0% | |
| TTATAAGGTCTCACTAACAT+TGG | + | chr5.4:50467864-50467883 | None:intergenic | 30.0% | |
| TTTGTGGAATTAGGTCAAAA+TGG | - | chr5.4:50467682-50467701 | MS.gene050073:CDS | 30.0% | |
| ! | ACCAAAGAAAACTTTTCCTT+TGG | - | chr5.4:50467401-50467420 | MS.gene050073:CDS | 30.0% |
| ! | AGTAGTGCTTATCAAGTTTT+GGG | - | chr5.4:50467630-50467649 | MS.gene050073:CDS | 30.0% |
| ! | TAGTAGTGCTTATCAAGTTT+TGG | - | chr5.4:50467629-50467648 | MS.gene050073:CDS | 30.0% |
| ! | TTTTCTTCAATACCCTGTTT+TGG | - | chr5.4:50467328-50467347 | MS.gene050073:CDS | 30.0% |
| !! | AAAAGGGTGTTAAGAAAGAT+TGG | - | chr5.4:50467301-50467320 | MS.gene050073:CDS | 30.0% |
| !! | ATGGATTAGTTTAGGTCAAA+AGG | - | chr5.4:50467284-50467303 | MS.gene050073:CDS | 30.0% |
| !! | CCACATAAATGGATTAGTTT+AGG | - | chr5.4:50467276-50467295 | MS.gene050073:CDS | 30.0% |
| !! | TGGAATTTGCTTCTTTTCTT+TGG | - | chr5.4:50467823-50467842 | MS.gene050073:CDS | 30.0% |
| !! | TGGATTAGTTTAGGTCAAAA+GGG | - | chr5.4:50467285-50467304 | MS.gene050073:CDS | 30.0% |
| !!! | TGCTACTAAGACTGTTTTTT+TGG | - | chr5.4:50467959-50467978 | MS.gene050073:CDS | 30.0% |
| !!! | TTCTTTGTGGTGGATTTTTT+GGG | + | chr5.4:50466757-50466776 | None:intergenic | 30.0% |
| !!! | TTTCTTTGTGGTGGATTTTT+TGG | + | chr5.4:50466758-50466777 | None:intergenic | 30.0% |
| AAGAGAATTGCTATGTAACG+AGG | + | chr5.4:50467126-50467145 | None:intergenic | 35.0% | |
| AATCAAAGTGAATGGGAAAC+TGG | - | chr5.4:50467501-50467520 | MS.gene050073:CDS | 35.0% | |
| AATGAATTTGCCTATGTTGG+TGG | - | chr5.4:50467890-50467909 | MS.gene050073:CDS | 35.0% | |
| ACAGCAATTAAAAGAGCCAA+AGG | + | chr5.4:50467420-50467439 | None:intergenic | 35.0% | |
| ACCAACATAGGCAAATTCAT+TGG | + | chr5.4:50467891-50467910 | None:intergenic | 35.0% | |
| ATATAATCGATGCAGAGAAC+AGG | + | chr5.4:50467069-50467088 | None:intergenic | 35.0% | |
| ATCAAAGTGAATGGGAAACT+GGG | - | chr5.4:50467502-50467521 | MS.gene050073:CDS | 35.0% | |
| ATGGTTTAATACACCTTGGT+TGG | - | chr5.4:50467701-50467720 | MS.gene050073:CDS | 35.0% | |
| CCATTAAAGAGCCTGAAAAT+GGG | + | chr5.4:50467002-50467021 | None:intergenic | 35.0% | |
| CTGAGATTATTGCTGGAAAA+TGG | - | chr5.4:50467541-50467560 | MS.gene050073:CDS | 35.0% | |
| GACCTAATTCCACAAAACTT+TGG | + | chr5.4:50467678-50467697 | None:intergenic | 35.0% | |
| TATAATCGATGCAGAGAACA+GGG | + | chr5.4:50467068-50467087 | None:intergenic | 35.0% | |
| TCCAAATGAAAGGGAAGATA+AGG | + | chr5.4:50466891-50466910 | None:intergenic | 35.0% | |
| TCCAATGAATTTGCCTATGT+TGG | - | chr5.4:50467887-50467906 | MS.gene050073:CDS | 35.0% | |
| TCCTTATCTTCCCTTTCATT+TGG | - | chr5.4:50466887-50466906 | MS.gene050073:CDS | 35.0% | |
| TGAAGAAGATGGGAATATAG+TGG | - | chr5.4:50468076-50468095 | MS.gene050073:CDS | 35.0% | |
| TGGTTTAATACACCTTGGTT+GGG | - | chr5.4:50467702-50467721 | MS.gene050073:CDS | 35.0% | |
| ! | CTTCTTTTCTTTGGTTGAGA+TGG | - | chr5.4:50467832-50467851 | MS.gene050073:CDS | 35.0% |
| ! | GCCAAAGGAAAAGTTTTCTT+TGG | + | chr5.4:50467405-50467424 | None:intergenic | 35.0% |
| ! | GGAATTTTGCCAAAGTTTTG+TGG | - | chr5.4:50467666-50467685 | MS.gene050073:CDS | 35.0% |
| ! | GGTGAAGTTTGAGATTAAAG+AGG | - | chr5.4:50468052-50468071 | MS.gene050073:CDS | 35.0% |
| !! | CCATTTTCAGGCTCTTTAAT+GGG | - | chr5.4:50467000-50467019 | MS.gene050073:CDS | 35.0% |
| !! | GATTAGGGTTAGTTTCTTTG+TGG | + | chr5.4:50466770-50466789 | None:intergenic | 35.0% |
| !! | TGCCAAAGTTTTGTGGAATT+AGG | - | chr5.4:50467673-50467692 | MS.gene050073:CDS | 35.0% |
| !!! | AAACGGTGGTTTTGTTATCT+GGG | - | chr5.4:50466960-50466979 | MS.gene050073:CDS | 35.0% |
| !!! | AAGACTGTTTTTTTGGTGAG+TGG | - | chr5.4:50467966-50467985 | MS.gene050073:CDS | 35.0% |
| !!! | ATTCCACTTTTTGCTGTTAC+TGG | - | chr5.4:50467465-50467484 | MS.gene050073:CDS | 35.0% |
| !!! | CTCATCTATTTTGAAGTAGC+CGG | - | chr5.4:50466802-50466821 | MS.gene050073:CDS | 35.0% |
| !!! | CTTTTCCCTGTTTTTGAATC+TGG | - | chr5.4:50467096-50467115 | MS.gene050073:CDS | 35.0% |
| !!! | GTGCTGTTTTATCAGCAATT+GGG | - | chr5.4:50467586-50467605 | MS.gene050073:CDS | 35.0% |
| !!! | TGCTTATCAAGTTTTGGGTA+TGG | - | chr5.4:50467635-50467654 | MS.gene050073:CDS | 35.0% |
| AAACACATCACCACCAACAT+AGG | + | chr5.4:50467903-50467922 | None:intergenic | 40.0% | |
| ATAACAAAACCACCGTTTCC+TGG | + | chr5.4:50466958-50466977 | None:intergenic | 40.0% | |
| CCCATTAAAGAGCCTGAAAA+TGG | + | chr5.4:50467003-50467022 | None:intergenic | 40.0% | |
| CGGCTACTTCAAAATAGATG+AGG | + | chr5.4:50466804-50466823 | None:intergenic | 40.0% | |
| CTGGAATGCTCCAAATGAAA+GGG | + | chr5.4:50466900-50466919 | None:intergenic | 40.0% | |
| GAATTAACCACCGTTTATCC+AGG | - | chr5.4:50466937-50466956 | MS.gene050073:CDS | 40.0% | |
| GAGGAATATGAGAGGGATTA+GGG | + | chr5.4:50466785-50466804 | None:intergenic | 40.0% | |
| GCCAACCAGATTCAAAAACA+GGG | + | chr5.4:50467104-50467123 | None:intergenic | 40.0% | |
| GGTACATGGAAATTCCTTAG+TGG | - | chr5.4:50467021-50467040 | MS.gene050073:CDS | 40.0% | |
| TCAGCAGTTATTAGAGCTTC+TGG | + | chr5.4:50466919-50466938 | None:intergenic | 40.0% | |
| TGAGGAATATGAGAGGGATT+AGG | + | chr5.4:50466786-50466805 | None:intergenic | 40.0% | |
| ! | AAATGGGCCGAAAGCTTTTT+CGG | + | chr5.4:50466986-50467005 | None:intergenic | 40.0% |
| ! | AGTGGTTTGATGACTTTAGG+TGG | - | chr5.4:50467984-50468003 | MS.gene050073:CDS | 40.0% |
| ! | GTGAGTGGTTTGATGACTTT+AGG | - | chr5.4:50467981-50468000 | MS.gene050073:CDS | 40.0% |
| ! | GTGGTTTGATGACTTTAGGT+GGG | - | chr5.4:50467985-50468004 | MS.gene050073:CDS | 40.0% |
| ! | TCTGGAATGCTCCAAATGAA+AGG | + | chr5.4:50466901-50466920 | None:intergenic | 40.0% |
| ! | TTGATGACTTTAGGTGGGAT+TGG | - | chr5.4:50467990-50468009 | MS.gene050073:CDS | 40.0% |
| !! | CCCATTTTCAGGCTCTTTAA+TGG | - | chr5.4:50466999-50467018 | MS.gene050073:CDS | 40.0% |
| !! | GTTTTGGGTATGGCTGAAAT+TGG | - | chr5.4:50467645-50467664 | MS.gene050073:CDS | 40.0% |
| !! | TAGGGTTAGTTTCTTTGTGG+TGG | + | chr5.4:50466767-50466786 | None:intergenic | 40.0% |
| !! | TATGTTGGTGGTGATGTGTT+TGG | - | chr5.4:50467902-50467921 | MS.gene050073:CDS | 40.0% |
| !!! | ATCTATTTTGAAGTAGCCGG+CGG | - | chr5.4:50466805-50466824 | MS.gene050073:CDS | 40.0% |
| !!! | GAAACGGTGGTTTTGTTATC+TGG | - | chr5.4:50466959-50466978 | MS.gene050073:CDS | 40.0% |
| !!! | GGTGCTGTTTTATCAGCAAT+TGG | - | chr5.4:50467585-50467604 | MS.gene050073:CDS | 40.0% |
| !!! | TCCCTGTTTTTGAATCTGGT+TGG | - | chr5.4:50467100-50467119 | MS.gene050073:CDS | 40.0% |
| !!! | TGTGGTGGATTTTTTGGGAA+TGG | + | chr5.4:50466752-50466771 | None:intergenic | 40.0% |
| ACCACCGTTTATCCAGGAAA+CGG | - | chr5.4:50466943-50466962 | MS.gene050073:CDS | 45.0% | |
| ACCACCGTTTCCTGGATAAA+CGG | + | chr5.4:50466950-50466969 | None:intergenic | 45.0% | |
| ATCTGGGCCGAAAAAGCTTT+CGG | - | chr5.4:50466976-50466995 | MS.gene050073:CDS | 45.0% | |
| CAAGCTGCTGAGATTATTGC+TGG | - | chr5.4:50467534-50467553 | MS.gene050073:CDS | 45.0% | |
| GATACCAAAATGCCCAACCA+AGG | + | chr5.4:50467717-50467736 | None:intergenic | 45.0% | |
| GCTCCAGTAACAGCAAAAAG+TGG | + | chr5.4:50467471-50467490 | None:intergenic | 45.0% | |
| GGCCAACCAGATTCAAAAAC+AGG | + | chr5.4:50467105-50467124 | None:intergenic | 45.0% | |
| GTGATGTGTTTGGTACCATC+TGG | - | chr5.4:50467912-50467931 | MS.gene050073:CDS | 45.0% | |
| ! | AAAGCTTTCGGCCCATTTTC+AGG | - | chr5.4:50466988-50467007 | MS.gene050073:CDS | 45.0% |
| ! | CAGGCTCTTTAATGGGTACA+TGG | - | chr5.4:50467007-50467026 | MS.gene050073:CDS | 45.0% |
| ! | TACACCTTGGTTGGGCATTT+TGG | - | chr5.4:50467710-50467729 | MS.gene050073:CDS | 45.0% |
| !!! | GGATTTTTTGGGAATGGTGG+TGG | + | chr5.4:50466746-50466765 | None:intergenic | 45.0% |
| !!! | GGTGGATTTTTTGGGAATGG+TGG | + | chr5.4:50466749-50466768 | None:intergenic | 45.0% |
| !!! | TTTTTTGGGAATGGTGGTGG+TGG | + | chr5.4:50466743-50466762 | None:intergenic | 45.0% |
| ACCGTTTATCCAGGAAACGG+TGG | - | chr5.4:50466946-50466965 | MS.gene050073:CDS | 50.0% | |
| ACCGTTTCCTGGATAAACGG+TGG | + | chr5.4:50466947-50466966 | None:intergenic | 50.0% | |
| ! | AAGCCGAGAAGTGCAAAGAG+AGG | + | chr5.4:50466868-50466887 | None:intergenic | 50.0% |
| ! | AGCCGAGAAGTGCAAAGAGA+GGG | + | chr5.4:50466867-50466886 | None:intergenic | 50.0% |
| GGCCCTCTCTTTGCACTTCT+CGG | - | chr5.4:50466862-50466881 | MS.gene050073:CDS | 55.0% | |
| ! | GAGAAGTGCAAAGAGAGGGC+CGG | + | chr5.4:50466863-50466882 | None:intergenic | 55.0% |
| !! | TTTGGGAATGGTGGTGGTGG+TGG | + | chr5.4:50466740-50466759 | None:intergenic | 55.0% |
| AAGTGCAAAGAGAGGGCCGG+CGG | + | chr5.4:50466860-50466879 | None:intergenic | 60.0% | |
| TGGTGGTTGGAGCGGTGGAA+AGG | + | chr5.4:50466717-50466736 | None:intergenic | 60.0% | |
| !! | AATGGTGGTGGTGGTGGTGG+TGG | + | chr5.4:50466734-50466753 | None:intergenic | 60.0% |
| GAAGTAGCCGGCGGCCCATA+CGG | - | chr5.4:50466814-50466833 | MS.gene050073:CDS | 65.0% | |
| TTCTTCGCCGTATGGGCCGC+CGG | + | chr5.4:50466824-50466843 | None:intergenic | 65.0% | |
| ! | ACGGCGGGTTCTTCGCCGTA+TGG | + | chr5.4:50466832-50466851 | None:intergenic | 65.0% |
| ! | CGGCGGGTTCTTCGCCGTAT+GGG | + | chr5.4:50466831-50466850 | None:intergenic | 65.0% |
| !! | GGGAATGGTGGTGGTGGTGG+TGG | + | chr5.4:50466737-50466756 | None:intergenic | 65.0% |
| !! | GGTGGTGGTGGTGGTTGGAG+CGG | + | chr5.4:50466725-50466744 | None:intergenic | 65.0% |
| !! | GTGGTGGTGGTGGTGGTGGT+TGG | + | chr5.4:50466730-50466749 | None:intergenic | 65.0% |
| GAACCCGCCGTTCAAGCCGC+CGG | - | chr5.4:50466841-50466860 | MS.gene050073:CDS | 70.0% | |
| GAGAGGGCCGGCGGCTTGAA+CGG | + | chr5.4:50466851-50466870 | None:intergenic | 70.0% | |
| !! | GGTGGTGGTGGTTGGAGCGG+TGG | + | chr5.4:50466722-50466741 | None:intergenic | 70.0% |
| AGGGCCGGCGGCTTGAACGG+CGG | + | chr5.4:50466848-50466867 | None:intergenic | 75.0% | |
| ! | GGGCCGGCGGCTTGAACGGC+GGG | + | chr5.4:50466847-50466866 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 50466700 | 50468109 | 50466700 | ID=MS.gene050073 |
| chr5.4 | mRNA | 50466700 | 50468109 | 50466700 | ID=MS.gene050073.t1;Parent=MS.gene050073 |
| chr5.4 | exon | 50466700 | 50468109 | 50466700 | ID=MS.gene050073.t1.exon1;Parent=MS.gene050073.t1 |
| chr5.4 | CDS | 50466700 | 50468109 | 50466700 | ID=cds.MS.gene050073.t1;Parent=MS.gene050073.t1 |
| Gene Sequence |
| Protein sequence |
