Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37668.t1 | XP_003592388.2 | 92.9 | 168 | 12 | 0 | 1 | 168 | 77 | 244 | 4.20E-80 | 307.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37668.t1 | Q9FLI1 | 46.1 | 165 | 79 | 2 | 1 | 155 | 4 | 168 | 1.3e-26 | 120.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37668.t1 | G7ID49 | 92.9 | 168 | 12 | 0 | 1 | 168 | 77 | 244 | 3.0e-80 | 307.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene37668.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene070993 | MS.gene37668 | PPI |
| MS.gene006365 | MS.gene37668 | PPI |
| MS.gene34423 | MS.gene37668 | PPI |
| MS.gene37667 | MS.gene37668 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37668.t1 | MTR_1g102370 | 92.857 | 168 | 12 | 0 | 1 | 168 | 77 | 244 | 7.30e-112 | 318 |
| MS.gene37668.t1 | MTR_0007s0380 | 54.762 | 168 | 72 | 2 | 1 | 166 | 73 | 238 | 2.61e-55 | 174 |
| MS.gene37668.t1 | MTR_0007s0330 | 39.306 | 173 | 95 | 3 | 1 | 167 | 20 | 188 | 6.11e-32 | 114 |
| MS.gene37668.t1 | MTR_0007s0330 | 40.645 | 155 | 85 | 2 | 1 | 149 | 8 | 161 | 9.64e-31 | 109 |
| MS.gene37668.t1 | MTR_1g102380 | 37.126 | 167 | 85 | 3 | 1 | 154 | 21 | 180 | 2.90e-28 | 103 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37668.t1 | AT5G51780 | 45.509 | 167 | 81 | 2 | 1 | 157 | 4 | 170 | 4.39e-37 | 126 |
| MS.gene37668.t1 | AT5G51780 | 45.509 | 167 | 81 | 2 | 1 | 157 | 27 | 193 | 9.09e-37 | 126 |
| MS.gene37668.t1 | AT4G25400 | 45.860 | 157 | 78 | 3 | 1 | 154 | 27 | 179 | 5.70e-35 | 121 |
| MS.gene37668.t1 | AT4G25410 | 42.857 | 168 | 80 | 5 | 3 | 157 | 47 | 211 | 5.90e-34 | 119 |
| MS.gene37668.t1 | AT4G25400 | 46.897 | 145 | 70 | 3 | 1 | 142 | 27 | 167 | 1.26e-32 | 115 |
| MS.gene37668.t1 | AT5G51790 | 42.424 | 165 | 87 | 4 | 1 | 157 | 29 | 193 | 3.58e-31 | 112 |
| MS.gene37668.t1 | AT5G51790 | 42.424 | 165 | 87 | 4 | 1 | 157 | 94 | 258 | 1.70e-30 | 112 |
| MS.gene37668.t1 | AT5G51790 | 44.000 | 150 | 76 | 4 | 1 | 142 | 29 | 178 | 1.33e-29 | 107 |
| MS.gene37668.t1 | AT5G51790 | 44.000 | 150 | 76 | 4 | 1 | 142 | 94 | 243 | 6.99e-29 | 107 |
| MS.gene37668.t1 | AT1G62975 | 33.708 | 178 | 94 | 3 | 1 | 154 | 76 | 253 | 7.68e-23 | 91.7 |
| MS.gene37668.t1 | AT1G12540 | 35.429 | 175 | 98 | 5 | 3 | 163 | 79 | 252 | 8.12e-22 | 89.0 |
| MS.gene37668.t1 | AT1G62975 | 34.337 | 166 | 85 | 3 | 1 | 142 | 76 | 241 | 1.04e-20 | 85.9 |
Find 31 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGAGGGAGAAGGGATCTA+AGG | 0.225427 | 1.1:-73395145 | None:intergenic |
| ATAAGAAGAAAATGGTTCAT+AGG | 0.350975 | 1.1:+73395075 | None:intergenic |
| AAATCACTTGAAGAAGAATA+TGG | 0.357120 | 1.1:+73395327 | MS.gene37668:CDS |
| TACTAGTACTTGCTTTATAG+AGG | 0.425326 | 1.1:+73395474 | MS.gene37668:CDS |
| GTACCTCACACTGCACACTA+TGG | 0.438771 | 1.1:-73395591 | None:intergenic |
| CTACAGTTCTATTATTCTTG+TGG | 0.453618 | 1.1:-73395438 | None:intergenic |
| AAGAGGGAGAAGGGATCTAA+GGG | 0.467872 | 1.1:-73395144 | None:intergenic |
| TAATCTGAACAACTAAATGA+TGG | 0.468144 | 1.1:-73395772 | None:intergenic |
| TAAGAAGAAAATGGTTCATA+GGG | 0.475156 | 1.1:+73395076 | None:intergenic |
| TAGATCTAACTGAGCTAAGA+AGG | 0.478104 | 1.1:+73395731 | MS.gene37668:CDS |
| GTTCCAGAAATTTAGAAAGT+GGG | 0.495839 | 1.1:-73395504 | None:intergenic |
| TGTTCCAGAAATTTAGAAAG+TGG | 0.503124 | 1.1:-73395505 | None:intergenic |
| GGGATCTAAGGGAAGCATGA+AGG | 0.513749 | 1.1:-73395133 | None:intergenic |
| TGGAAGAACTAAGTTACAAG+AGG | 0.531556 | 1.1:+73395347 | MS.gene37668:CDS |
| GAAGCAAAGGAGACAAGAAA+TGG | 0.533586 | 1.1:+73395106 | MS.gene37668:CDS |
| TCTCCCTCTTCACTTCATCA+AGG | 0.533778 | 1.1:+73395157 | MS.gene37668:CDS |
| TCACCTTGATGAAGTGAAGA+GGG | 0.538188 | 1.1:-73395160 | None:intergenic |
| GATGAAGTGAAGAGGGAGAA+GGG | 0.546212 | 1.1:-73395153 | None:intergenic |
| TTCCAGAAATTTAGAAAGTG+GGG | 0.547614 | 1.1:-73395503 | None:intergenic |
| AGTACTTGCTTTATAGAGGA+AGG | 0.558027 | 1.1:+73395478 | MS.gene37668:CDS |
| CACAAGAATAATAGAACTGT+AGG | 0.565202 | 1.1:+73395439 | MS.gene37668:CDS |
| ACTAAATGATGGATTCACAT+TGG | 0.566776 | 1.1:-73395761 | None:intergenic |
| ATAGGGAAATTGAGAAGCAA+AGG | 0.575199 | 1.1:+73395093 | MS.gene37668:CDS |
| TGATGAAGTGAAGAGGGAGA+AGG | 0.577558 | 1.1:-73395154 | None:intergenic |
| CTCAGTTAGATCTACACTGT+CGG | 0.580625 | 1.1:-73395722 | None:intergenic |
| GGATCTAAGGGAAGCATGAA+GGG | 0.581442 | 1.1:-73395132 | None:intergenic |
| TTCACCTTGATGAAGTGAAG+AGG | 0.603402 | 1.1:-73395161 | None:intergenic |
| TCCAGAAATTTAGAAAGTGG+GGG | 0.626747 | 1.1:-73395502 | None:intergenic |
| GGAAGAACTAAGTTACAAGA+GGG | 0.642455 | 1.1:+73395348 | MS.gene37668:CDS |
| ACTCCATAGTGTGCAGTGTG+AGG | 0.658037 | 1.1:+73395588 | MS.gene37668:CDS |
| TAGTGAAACTACAAGAAACA+TGG | 0.661078 | 1.1:-73395414 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATCATTAATTAAAGTTTTCT+AGG | + | chr1.1:73395203-73395222 | MS.gene37668:intron | 15.0% |
| !! | TCTCTTCTATTATAACTTTA+GGG | + | chr1.1:73395232-73395251 | MS.gene37668:intron | 20.0% |
| !! | TTCTCTTCTATTATAACTTT+AGG | + | chr1.1:73395231-73395250 | MS.gene37668:intron | 20.0% |
| !!! | AAACATGGCTTTTATTTTTA+AGG | - | chr1.1:73395402-73395421 | None:intergenic | 20.0% |
| ! | AAATCACTTGAAGAAGAATA+TGG | + | chr1.1:73395327-73395346 | MS.gene37668:CDS | 25.0% |
| ! | TAATCTGAACAACTAAATGA+TGG | - | chr1.1:73395775-73395794 | None:intergenic | 25.0% |
| !!! | TTATTGTTTCTGATCTAACA+GGG | + | chr1.1:73395259-73395278 | MS.gene37668:intron | 25.0% |
| !!! | TTTATTGTTTCTGATCTAAC+AGG | + | chr1.1:73395258-73395277 | MS.gene37668:intron | 25.0% |
| CACAAGAATAATAGAACTGT+AGG | + | chr1.1:73395439-73395458 | MS.gene37668:CDS | 30.0% | |
| CTACAGTTCTATTATTCTTG+TGG | - | chr1.1:73395441-73395460 | None:intergenic | 30.0% | |
| GTTCCAGAAATTTAGAAAGT+GGG | - | chr1.1:73395507-73395526 | None:intergenic | 30.0% | |
| TACTAGTACTTGCTTTATAG+AGG | + | chr1.1:73395474-73395493 | MS.gene37668:CDS | 30.0% | |
| TAGTGAAACTACAAGAAACA+TGG | - | chr1.1:73395417-73395436 | None:intergenic | 30.0% | |
| TGTTCCAGAAATTTAGAAAG+TGG | - | chr1.1:73395508-73395527 | None:intergenic | 30.0% | |
| TTCCAGAAATTTAGAAAGTG+GGG | - | chr1.1:73395506-73395525 | None:intergenic | 30.0% | |
| ! | TGCTTTTCTATTCAAGTCAA+TGG | + | chr1.1:73395559-73395578 | MS.gene37668:CDS | 30.0% |
| !! | ACTAAATGATGGATTCACAT+TGG | - | chr1.1:73395764-73395783 | None:intergenic | 30.0% |
| !!! | GGCTTTTATTTTTAAGGCTA+GGG | - | chr1.1:73395396-73395415 | None:intergenic | 30.0% |
| !!! | TGGCTTTTATTTTTAAGGCT+AGG | - | chr1.1:73395397-73395416 | None:intergenic | 30.0% |
| AGTACTTGCTTTATAGAGGA+AGG | + | chr1.1:73395478-73395497 | MS.gene37668:CDS | 35.0% | |
| ATAGGGAAATTGAGAAGCAA+AGG | + | chr1.1:73395093-73395112 | MS.gene37668:CDS | 35.0% | |
| CTGGAACAACTGATGAAAAA+TGG | + | chr1.1:73395520-73395539 | MS.gene37668:CDS | 35.0% | |
| GGAAGAACTAAGTTACAAGA+GGG | + | chr1.1:73395348-73395367 | MS.gene37668:CDS | 35.0% | |
| TAGATCTAACTGAGCTAAGA+AGG | + | chr1.1:73395731-73395750 | MS.gene37668:CDS | 35.0% | |
| TCCAGAAATTTAGAAAGTGG+GGG | - | chr1.1:73395505-73395524 | None:intergenic | 35.0% | |
| TGGAAGAACTAAGTTACAAG+AGG | + | chr1.1:73395347-73395366 | MS.gene37668:CDS | 35.0% | |
| ! | TATTGAGCTTGATGATTCAC+AGG | + | chr1.1:73395690-73395709 | MS.gene37668:intron | 35.0% |
| CTCAGTTAGATCTACACTGT+CGG | - | chr1.1:73395725-73395744 | None:intergenic | 40.0% | |
| GAAGCAAAGGAGACAAGAAA+TGG | + | chr1.1:73395106-73395125 | MS.gene37668:CDS | 40.0% | |
| TCACCTTGATGAAGTGAAGA+GGG | - | chr1.1:73395163-73395182 | None:intergenic | 40.0% | |
| TCCCCCACTTTCTAAATTTC+TGG | + | chr1.1:73395501-73395520 | MS.gene37668:CDS | 40.0% | |
| TTCACCTTGATGAAGTGAAG+AGG | - | chr1.1:73395164-73395183 | None:intergenic | 40.0% | |
| AAGAGGGAGAAGGGATCTAA+GGG | - | chr1.1:73395147-73395166 | None:intergenic | 45.0% | |
| GATGAAGTGAAGAGGGAGAA+GGG | - | chr1.1:73395156-73395175 | None:intergenic | 45.0% | |
| TCTCCCTCTTCACTTCATCA+AGG | + | chr1.1:73395157-73395176 | MS.gene37668:CDS | 45.0% | |
| TGATGAAGTGAAGAGGGAGA+AGG | - | chr1.1:73395157-73395176 | None:intergenic | 45.0% | |
| ! | GGATCTAAGGGAAGCATGAA+GGG | - | chr1.1:73395135-73395154 | None:intergenic | 45.0% |
| ACTCCATAGTGTGCAGTGTG+AGG | + | chr1.1:73395588-73395607 | MS.gene37668:CDS | 50.0% | |
| GAAGAGGGAGAAGGGATCTA+AGG | - | chr1.1:73395148-73395167 | None:intergenic | 50.0% | |
| GTACCTCACACTGCACACTA+TGG | - | chr1.1:73395594-73395613 | None:intergenic | 50.0% | |
| ! | GGGATCTAAGGGAAGCATGA+AGG | - | chr1.1:73395136-73395155 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 73395086 | 73395795 | 73395086 | ID=MS.gene37668 |
| chr1.1 | mRNA | 73395086 | 73395795 | 73395086 | ID=MS.gene37668.t1;Parent=MS.gene37668 |
| chr1.1 | exon | 73395086 | 73395178 | 73395086 | ID=MS.gene37668.t1.exon1;Parent=MS.gene37668.t1 |
| chr1.1 | CDS | 73395086 | 73395178 | 73395086 | ID=cds.MS.gene37668.t1;Parent=MS.gene37668.t1 |
| chr1.1 | exon | 73395280 | 73395609 | 73395280 | ID=MS.gene37668.t1.exon2;Parent=MS.gene37668.t1 |
| chr1.1 | CDS | 73395280 | 73395609 | 73395280 | ID=cds.MS.gene37668.t1;Parent=MS.gene37668.t1 |
| chr1.1 | exon | 73395712 | 73395795 | 73395712 | ID=MS.gene37668.t1.exon3;Parent=MS.gene37668.t1 |
| chr1.1 | CDS | 73395712 | 73395795 | 73395712 | ID=cds.MS.gene37668.t1;Parent=MS.gene37668.t1 |
| Gene Sequence |
| Protein sequence |
