Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37734.t1 | XP_003620169.1 | 97.5 | 203 | 5 | 0 | 1 | 203 | 1 | 203 | 9.40E-111 | 409.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37734.t1 | Q9LMU2 | 48.3 | 207 | 88 | 8 | 3 | 203 | 2 | 195 | 1.6e-42 | 174.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37734.t1 | G7KKP1 | 97.5 | 203 | 5 | 0 | 1 | 203 | 1 | 203 | 6.8e-111 | 409.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05221 | MS.gene37734 | 0.924426 | 7.16E-90 | -1.69E-46 |
| MS.gene05224 | MS.gene37734 | 0.920689 | 9.31E-88 | -1.69E-46 |
| MS.gene055465 | MS.gene37734 | 0.805407 | 1.36E-49 | -1.69E-46 |
| MS.gene055469 | MS.gene37734 | 0.807041 | 6.13E-50 | -1.69E-46 |
| MS.gene059170 | MS.gene37734 | 0.805557 | 1.26E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37734.t1 | MTR_6g078070 | 97.537 | 203 | 5 | 0 | 1 | 203 | 1 | 203 | 2.00e-147 | 408 |
| MS.gene37734.t1 | MTR_6g078100 | 82.039 | 206 | 33 | 2 | 1 | 203 | 1 | 205 | 1.65e-118 | 335 |
| MS.gene37734.t1 | MTR_6g078290 | 44.444 | 207 | 98 | 8 | 5 | 201 | 11 | 210 | 3.14e-45 | 149 |
| MS.gene37734.t1 | MTR_7g037410 | 41.935 | 186 | 94 | 7 | 23 | 203 | 26 | 202 | 6.37e-37 | 127 |
| MS.gene37734.t1 | MTR_6g078120 | 40.933 | 193 | 99 | 7 | 21 | 203 | 26 | 213 | 3.01e-35 | 124 |
| MS.gene37734.t1 | MTR_6g078140 | 40.415 | 193 | 100 | 7 | 21 | 203 | 26 | 213 | 6.72e-34 | 120 |
| MS.gene37734.t1 | MTR_6g078040 | 66.292 | 89 | 25 | 3 | 93 | 177 | 1 | 88 | 7.11e-32 | 111 |
| MS.gene37734.t1 | MTR_6g078280 | 36.923 | 195 | 104 | 7 | 21 | 203 | 25 | 212 | 2.08e-30 | 111 |
| MS.gene37734.t1 | MTR_6g078250 | 40.000 | 175 | 93 | 7 | 24 | 191 | 25 | 194 | 9.98e-30 | 109 |
| MS.gene37734.t1 | MTR_6g078260 | 32.961 | 179 | 109 | 6 | 26 | 203 | 31 | 199 | 1.62e-23 | 93.2 |
| MS.gene37734.t1 | MTR_3g106585 | 33.014 | 209 | 113 | 9 | 5 | 203 | 6 | 197 | 1.01e-22 | 91.3 |
| MS.gene37734.t1 | MTR_6g065460 | 35.211 | 213 | 119 | 9 | 1 | 203 | 1 | 204 | 2.07e-22 | 90.5 |
| MS.gene37734.t1 | MTR_3g106620 | 32.057 | 209 | 117 | 10 | 5 | 203 | 6 | 199 | 9.42e-20 | 83.6 |
| MS.gene37734.t1 | MTR_2g072980 | 31.188 | 202 | 127 | 6 | 3 | 203 | 9 | 199 | 1.39e-19 | 82.8 |
| MS.gene37734.t1 | MTR_0100s0150 | 31.884 | 207 | 122 | 10 | 4 | 202 | 5 | 200 | 5.04e-19 | 81.3 |
| MS.gene37734.t1 | MTR_2g073010 | 32.039 | 206 | 119 | 8 | 3 | 203 | 9 | 198 | 6.85e-19 | 80.9 |
| MS.gene37734.t1 | MTR_0302s0030 | 30.097 | 206 | 128 | 9 | 4 | 202 | 5 | 201 | 1.49e-17 | 77.4 |
| MS.gene37734.t1 | MTR_6g059650 | 31.720 | 186 | 118 | 7 | 20 | 202 | 22 | 201 | 6.51e-17 | 75.9 |
| MS.gene37734.t1 | MTR_6g059680 | 34.595 | 185 | 114 | 6 | 22 | 203 | 28 | 208 | 6.73e-17 | 76.3 |
| MS.gene37734.t1 | MTR_3g463590 | 30.233 | 172 | 115 | 4 | 18 | 189 | 21 | 187 | 2.68e-16 | 74.3 |
| MS.gene37734.t1 | MTR_0084s0030 | 28.293 | 205 | 131 | 9 | 4 | 202 | 7 | 201 | 2.97e-16 | 73.9 |
| MS.gene37734.t1 | MTR_6g059530 | 31.553 | 206 | 127 | 7 | 1 | 203 | 1 | 195 | 3.57e-16 | 73.9 |
| MS.gene37734.t1 | MTR_6g059730 | 33.333 | 183 | 112 | 6 | 24 | 203 | 29 | 204 | 6.62e-16 | 73.6 |
| MS.gene37734.t1 | MTR_6g059410 | 31.553 | 206 | 126 | 8 | 1 | 203 | 1 | 194 | 1.76e-15 | 72.0 |
| MS.gene37734.t1 | MTR_8g461050 | 32.843 | 204 | 117 | 13 | 10 | 202 | 7 | 201 | 8.36e-15 | 70.1 |
| MS.gene37734.t1 | MTR_6g059760 | 30.622 | 209 | 126 | 9 | 1 | 200 | 1 | 199 | 1.74e-13 | 66.6 |
| MS.gene37734.t1 | MTR_8g060550 | 28.426 | 197 | 122 | 10 | 4 | 190 | 2 | 189 | 2.37e-13 | 66.2 |
| MS.gene37734.t1 | MTR_8g461690 | 28.426 | 197 | 122 | 10 | 4 | 190 | 2 | 189 | 2.37e-13 | 66.2 |
| MS.gene37734.t1 | MTR_6g009563 | 30.693 | 202 | 124 | 9 | 10 | 203 | 10 | 203 | 4.40e-12 | 62.8 |
| MS.gene37734.t1 | MTR_6g009650 | 30.882 | 204 | 127 | 9 | 6 | 203 | 7 | 202 | 4.65e-12 | 62.8 |
| MS.gene37734.t1 | MTR_6g066210 | 28.846 | 208 | 132 | 8 | 5 | 203 | 4 | 204 | 5.86e-12 | 62.8 |
| MS.gene37734.t1 | MTR_6g017225 | 30.435 | 161 | 102 | 6 | 6 | 161 | 7 | 162 | 9.58e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37734.t1 | AT1G17860 | 48.309 | 207 | 88 | 8 | 3 | 203 | 2 | 195 | 2.36e-54 | 172 |
| MS.gene37734.t1 | AT1G73260 | 37.017 | 181 | 106 | 5 | 26 | 203 | 30 | 205 | 2.93e-29 | 108 |
| MS.gene37734.t1 | AT1G73325 | 33.659 | 205 | 120 | 7 | 4 | 203 | 10 | 203 | 4.38e-23 | 92.8 |
Find 40 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTTAGGGTTGTGTTTAAA+AGG | 0.160681 | 6.3:-72046662 | MS.gene37734:CDS |
| GTGATCAAATTCCATCATTT+AGG | 0.169249 | 6.3:-72046677 | MS.gene37734:CDS |
| ACACCTTGTTTAGCATTATA+AGG | 0.259304 | 6.3:+72046990 | None:intergenic |
| GTATGGCAACTGTTGGTAAT+TGG | 0.271386 | 6.3:-72046830 | MS.gene37734:CDS |
| GAACCAGTTCTTGACATTTC+AGG | 0.287884 | 6.3:-72047176 | MS.gene37734:CDS |
| GCTTCTCTTGAACAACATAA+AGG | 0.342977 | 6.3:+72047048 | None:intergenic |
| TGATCAAATTCCATCATTTA+GGG | 0.346172 | 6.3:-72046676 | MS.gene37734:CDS |
| CAAACTTTGTGTAGGGAACT+AGG | 0.366708 | 6.3:-72046741 | MS.gene37734:CDS |
| CTTCTCTTGAACAACATAAA+GGG | 0.369437 | 6.3:+72047049 | None:intergenic |
| AGGGTTGTTTGTTGATGATA+AGG | 0.370874 | 6.3:-72046721 | MS.gene37734:CDS |
| AATGTCCTCAAACACAAGTT+TGG | 0.377880 | 6.3:-72046917 | MS.gene37734:CDS |
| TTACCAACAGTTGCCATACT+TGG | 0.386300 | 6.3:+72046834 | None:intergenic |
| CAACATAAAGGGGACATGTT+TGG | 0.398228 | 6.3:+72047060 | None:intergenic |
| AGCTTCCAAACTTGTGTTTG+AGG | 0.408512 | 6.3:+72046912 | None:intergenic |
| GGGTTGTTTGTTGATGATAA+GGG | 0.441383 | 6.3:-72046720 | MS.gene37734:CDS |
| TTAAACACAACCCTAAATGA+TGG | 0.447080 | 6.3:+72046666 | None:intergenic |
| TGTAGAAGGTAATCCAAGTA+TGG | 0.452203 | 6.3:-72046847 | MS.gene37734:CDS |
| ATTTAGGGTTGTGTTTAAAA+GGG | 0.479397 | 6.3:-72046661 | MS.gene37734:CDS |
| GAAATGTCAAGAACTGGTTC+AGG | 0.479615 | 6.3:+72047179 | None:intergenic |
| TGACATTTGCAAGCTTCAGC+AGG | 0.493041 | 6.3:+72046762 | None:intergenic |
| AAGCTTCTTAAAGAGTTGAC+AGG | 0.493248 | 6.3:-72046894 | MS.gene37734:CDS |
| GCAAATGTCAAACTTTGTGT+AGG | 0.501531 | 6.3:-72046749 | MS.gene37734:CDS |
| TCAGGCAAACAAGTGACAAC+TGG | 0.504943 | 6.3:-72047158 | MS.gene37734:CDS |
| TTGCCTGAAATGTCAAGAAC+TGG | 0.505604 | 6.3:+72047173 | None:intergenic |
| CAAATGTCAAACTTTGTGTA+GGG | 0.511038 | 6.3:-72046748 | MS.gene37734:CDS |
| TTACCAGTCATAAGAGGTAA+AGG | 0.515215 | 6.3:-72047119 | MS.gene37734:CDS |
| AAACATGAAAACCTCACTCT+TGG | 0.517251 | 6.3:-72047249 | None:intergenic |
| TTAAAGAGTTGACAGGGGTG+TGG | 0.531517 | 6.3:-72046887 | MS.gene37734:CDS |
| GAGAAGCTTGAAGTAAAGAA+TGG | 0.531990 | 6.3:-72047032 | MS.gene37734:CDS |
| AAACTTTGTGTAGGGAACTA+GGG | 0.532296 | 6.3:-72046740 | MS.gene37734:CDS |
| GTCATAAGAGGTAAAGGTGG+TGG | 0.556783 | 6.3:-72047113 | MS.gene37734:CDS |
| CCACCTTTACCTCTTATGAC+TGG | 0.557512 | 6.3:+72047116 | None:intergenic |
| AGCTTCTTAAAGAGTTGACA+GGG | 0.566536 | 6.3:-72046893 | MS.gene37734:CDS |
| TTCTCTTGAACAACATAAAG+GGG | 0.573118 | 6.3:+72047050 | None:intergenic |
| AATCCAAGTATGGCAACTGT+TGG | 0.585675 | 6.3:-72046837 | MS.gene37734:CDS |
| ACACCTTATAATGCTAAACA+AGG | 0.651507 | 6.3:-72046993 | MS.gene37734:CDS |
| GGTGGAAAATGCCAAGAGTG+AGG | 0.651966 | 6.3:+72047238 | None:intergenic |
| CCAGTCATAAGAGGTAAAGG+TGG | 0.660270 | 6.3:-72047116 | MS.gene37734:CDS |
| GGTTTAACAGTTGCAAACCA+TGG | 0.676406 | 6.3:-72047092 | MS.gene37734:CDS |
| GCTTCTTAAAGAGTTGACAG+GGG | 0.746376 | 6.3:-72046892 | MS.gene37734:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACATTTAGTTTTTGTTACAA+AGG | + | chr6.3:72046969-72046988 | None:intergenic | 20.0% |
| ! | TGATCAAATTCCATCATTTA+GGG | - | chr6.3:72047225-72047244 | MS.gene37734:CDS | 25.0% |
| !! | ATTTAGGGTTGTGTTTAAAA+GGG | - | chr6.3:72047240-72047259 | MS.gene37734:CDS | 25.0% |
| !! | TATATTTTACCAGTCATAAG+AGG | - | chr6.3:72046776-72046795 | MS.gene37734:CDS | 25.0% |
| ACACCTTATAATGCTAAACA+AGG | - | chr6.3:72046908-72046927 | MS.gene37734:CDS | 30.0% | |
| CAAATGTCAAACTTTGTGTA+GGG | - | chr6.3:72047153-72047172 | MS.gene37734:CDS | 30.0% | |
| CTTCTCTTGAACAACATAAA+GGG | + | chr6.3:72046855-72046874 | None:intergenic | 30.0% | |
| GTGATCAAATTCCATCATTT+AGG | - | chr6.3:72047224-72047243 | MS.gene37734:CDS | 30.0% | |
| TTAAACACAACCCTAAATGA+TGG | + | chr6.3:72047238-72047257 | None:intergenic | 30.0% | |
| TTCTCTTGAACAACATAAAG+GGG | + | chr6.3:72046854-72046873 | None:intergenic | 30.0% | |
| ! | ACACCTTGTTTAGCATTATA+AGG | + | chr6.3:72046914-72046933 | None:intergenic | 30.0% |
| ! | CATTTAGGGTTGTGTTTAAA+AGG | - | chr6.3:72047239-72047258 | MS.gene37734:CDS | 30.0% |
| !! | TGCAATAGTTTTGCAAATGA+AGG | + | chr6.3:72046699-72046718 | None:intergenic | 30.0% |
| AAACTTTGTGTAGGGAACTA+GGG | - | chr6.3:72047161-72047180 | MS.gene37734:CDS | 35.0% | |
| AAGCTTCTTAAAGAGTTGAC+AGG | - | chr6.3:72047007-72047026 | MS.gene37734:CDS | 35.0% | |
| AATGAAGGCTAAAAAGATGG+TGG | + | chr6.3:72046684-72046703 | None:intergenic | 35.0% | |
| AATGTCCTCAAACACAAGTT+TGG | - | chr6.3:72046984-72047003 | MS.gene37734:CDS | 35.0% | |
| AGCTTCTTAAAGAGTTGACA+GGG | - | chr6.3:72047008-72047027 | MS.gene37734:CDS | 35.0% | |
| GAGAAGCTTGAAGTAAAGAA+TGG | - | chr6.3:72046869-72046888 | MS.gene37734:CDS | 35.0% | |
| GCAAATGAAGGCTAAAAAGA+TGG | + | chr6.3:72046687-72046706 | None:intergenic | 35.0% | |
| GCAAATGTCAAACTTTGTGT+AGG | - | chr6.3:72047152-72047171 | MS.gene37734:CDS | 35.0% | |
| GCTTCTCTTGAACAACATAA+AGG | + | chr6.3:72046856-72046875 | None:intergenic | 35.0% | |
| TGTAGAAGGTAATCCAAGTA+TGG | - | chr6.3:72047054-72047073 | MS.gene37734:CDS | 35.0% | |
| TTACCAGTCATAAGAGGTAA+AGG | - | chr6.3:72046782-72046801 | MS.gene37734:CDS | 35.0% | |
| !! | AGGGTTGTTTGTTGATGATA+AGG | - | chr6.3:72047180-72047199 | MS.gene37734:CDS | 35.0% |
| !! | ATGTTTGGTTGTTTTCACCA+TGG | + | chr6.3:72046829-72046848 | None:intergenic | 35.0% |
| !! | GGGTTGTTTGTTGATGATAA+GGG | - | chr6.3:72047181-72047200 | MS.gene37734:CDS | 35.0% |
| AATCCAAGTATGGCAACTGT+TGG | - | chr6.3:72047064-72047083 | MS.gene37734:CDS | 40.0% | |
| AGCTTCCAAACTTGTGTTTG+AGG | + | chr6.3:72046992-72047011 | None:intergenic | 40.0% | |
| CAAACTTTGTGTAGGGAACT+AGG | - | chr6.3:72047160-72047179 | MS.gene37734:CDS | 40.0% | |
| CAACATAAAGGGGACATGTT+TGG | + | chr6.3:72046844-72046863 | None:intergenic | 40.0% | |
| GAACCAGTTCTTGACATTTC+AGG | - | chr6.3:72046725-72046744 | MS.gene37734:CDS | 40.0% | |
| GCTTCTTAAAGAGTTGACAG+GGG | - | chr6.3:72047009-72047028 | MS.gene37734:CDS | 40.0% | |
| GGTTTAACAGTTGCAAACCA+TGG | - | chr6.3:72046809-72046828 | MS.gene37734:CDS | 40.0% | |
| TTACCAACAGTTGCCATACT+TGG | + | chr6.3:72047070-72047089 | None:intergenic | 40.0% | |
| TTGCCTGAAATGTCAAGAAC+TGG | + | chr6.3:72046731-72046750 | None:intergenic | 40.0% | |
| ! | GAAATGTCAAGAACTGGTTC+AGG | + | chr6.3:72046725-72046744 | None:intergenic | 40.0% |
| !! | GTATGGCAACTGTTGGTAAT+TGG | - | chr6.3:72047071-72047090 | MS.gene37734:CDS | 40.0% |
| CCACCTTTACCTCTTATGAC+TGG | + | chr6.3:72046788-72046807 | None:intergenic | 45.0% | |
| CCAGTCATAAGAGGTAAAGG+TGG | - | chr6.3:72046785-72046804 | MS.gene37734:CDS | 45.0% | |
| GTCATAAGAGGTAAAGGTGG+TGG | - | chr6.3:72046788-72046807 | MS.gene37734:CDS | 45.0% | |
| TCAGGCAAACAAGTGACAAC+TGG | - | chr6.3:72046743-72046762 | MS.gene37734:CDS | 45.0% | |
| TGACATTTGCAAGCTTCAGC+AGG | + | chr6.3:72047142-72047161 | None:intergenic | 45.0% | |
| TTAAAGAGTTGACAGGGGTG+TGG | - | chr6.3:72047014-72047033 | MS.gene37734:CDS | 45.0% | |
| !!! | GGGTGTGGTTTTTAGCTACA+GGG | - | chr6.3:72047029-72047048 | MS.gene37734:CDS | 45.0% |
| !!! | GGTGTGGTTTTTAGCTACAG+GGG | - | chr6.3:72047030-72047049 | MS.gene37734:CDS | 45.0% |
| !!! | GTGTGGTTTTTAGCTACAGG+GGG | - | chr6.3:72047031-72047050 | MS.gene37734:CDS | 45.0% |
| GGTGGAAAATGCCAAGAGTG+AGG | + | chr6.3:72046666-72046685 | None:intergenic | 50.0% | |
| ! | TTAGCTACAGGGGGTGTAGA+AGG | - | chr6.3:72047040-72047059 | MS.gene37734:CDS | 50.0% |
| !!! | GGGGTGTGGTTTTTAGCTAC+AGG | - | chr6.3:72047028-72047047 | MS.gene37734:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 72046656 | 72047267 | 72046656 | ID=MS.gene37734 |
| chr6.3 | mRNA | 72046656 | 72047267 | 72046656 | ID=MS.gene37734.t1;Parent=MS.gene37734 |
| chr6.3 | exon | 72046656 | 72047267 | 72046656 | ID=MS.gene37734.t1.exon1;Parent=MS.gene37734.t1 |
| chr6.3 | CDS | 72046656 | 72047267 | 72046656 | ID=cds.MS.gene37734.t1;Parent=MS.gene37734.t1 |
| Gene Sequence |
| Protein sequence |
