Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05224.t1 | XP_003620169.1 | 96.6 | 203 | 7 | 0 | 1 | 203 | 1 | 203 | 5.20E-109 | 403.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05224.t1 | Q9LMU2 | 50.3 | 187 | 78 | 7 | 19 | 203 | 22 | 195 | 4.6e-42 | 172.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05224.t1 | G7KKP1 | 96.6 | 203 | 7 | 0 | 1 | 203 | 1 | 203 | 3.7e-109 | 403.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05221 | MS.gene05224 | 0.925158 | 2.68E-90 | -1.69E-46 |
| MS.gene05222 | MS.gene05224 | 0.801695 | 7.98E-49 | -1.69E-46 |
| MS.gene05224 | MS.gene055469 | 0.819509 | 1.12E-52 | -1.69E-46 |
| MS.gene05224 | MS.gene058720 | 0.846941 | 1.58E-59 | -1.69E-46 |
| MS.gene05224 | MS.gene064243 | 0.815683 | 8.15E-52 | -1.69E-46 |
| MS.gene05224 | MS.gene069441 | 0.912303 | 2.27E-83 | -1.69E-46 |
| MS.gene05224 | MS.gene06949 | 0.807869 | 4.09E-50 | -1.69E-46 |
| MS.gene05224 | MS.gene070339 | 0.806922 | 6.50E-50 | -1.69E-46 |
| MS.gene05224 | MS.gene07382 | 0.826542 | 2.56E-54 | -1.69E-46 |
| MS.gene05224 | MS.gene21416 | 0.813349 | 2.68E-51 | -1.69E-46 |
| MS.gene05224 | MS.gene27350 | 0.809572 | 1.77E-50 | -1.69E-46 |
| MS.gene05224 | MS.gene28979 | 0.800568 | 1.36E-48 | -1.69E-46 |
| MS.gene05224 | MS.gene29355 | 0.800705 | 1.27E-48 | -1.69E-46 |
| MS.gene05224 | MS.gene34485 | 0.808493 | 3.02E-50 | -1.69E-46 |
| MS.gene05224 | MS.gene36285 | 0.801013 | 1.10E-48 | -1.69E-46 |
| MS.gene05224 | MS.gene37734 | 0.920689 | 9.31E-88 | -1.69E-46 |
| MS.gene05224 | MS.gene41427 | 0.829808 | 4.18E-55 | -1.69E-46 |
| MS.gene05224 | MS.gene45281 | 0.801575 | 8.44E-49 | -1.69E-46 |
| MS.gene05224 | MS.gene49467 | 0.822328 | 2.51E-53 | -1.69E-46 |
| MS.gene05224 | MS.gene51171 | 0.812175 | 4.84E-51 | -1.69E-46 |
| MS.gene05224 | MS.gene51384 | 0.819143 | 1.36E-52 | -1.69E-46 |
| MS.gene05224 | MS.gene56476 | 0.805049 | 1.61E-49 | -1.69E-46 |
| MS.gene05224 | MS.gene63229 | 0.812995 | 3.20E-51 | -1.69E-46 |
| MS.gene05224 | MS.gene66928 | 0.809625 | 1.73E-50 | -1.69E-46 |
| MS.gene05224 | MS.gene67881 | 0.816002 | 6.92E-52 | -1.69E-46 |
| MS.gene05224 | MS.gene68106 | 0.808515 | 2.98E-50 | -1.69E-46 |
| MS.gene05224 | MS.gene68420 | 0.806479 | 8.06E-50 | -1.69E-46 |
| MS.gene05224 | MS.gene72170 | 0.807355 | 5.26E-50 | -1.69E-46 |
| MS.gene05224 | MS.gene73245 | 0.805254 | 1.46E-49 | -1.69E-46 |
| MS.gene05224 | MS.gene86822 | 0.803199 | 3.91E-49 | -1.69E-46 |
| MS.gene05224 | MS.gene88718 | 0.807667 | 4.52E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05224.t1 | MTR_6g078070 | 96.552 | 203 | 7 | 0 | 1 | 203 | 1 | 203 | 5.05e-145 | 402 |
| MS.gene05224.t1 | MTR_6g078100 | 81.068 | 206 | 35 | 2 | 1 | 203 | 1 | 205 | 6.23e-116 | 328 |
| MS.gene05224.t1 | MTR_6g078290 | 42.927 | 205 | 100 | 6 | 1 | 191 | 1 | 202 | 2.28e-46 | 152 |
| MS.gene05224.t1 | MTR_7g037410 | 42.473 | 186 | 93 | 7 | 23 | 203 | 26 | 202 | 1.83e-38 | 132 |
| MS.gene05224.t1 | MTR_6g078120 | 41.436 | 181 | 93 | 5 | 21 | 191 | 26 | 203 | 6.88e-35 | 122 |
| MS.gene05224.t1 | MTR_6g078140 | 40.884 | 181 | 94 | 5 | 21 | 191 | 26 | 203 | 1.41e-33 | 119 |
| MS.gene05224.t1 | MTR_6g078040 | 70.000 | 80 | 22 | 2 | 93 | 171 | 1 | 79 | 7.58e-32 | 111 |
| MS.gene05224.t1 | MTR_6g078280 | 36.923 | 195 | 104 | 7 | 21 | 203 | 25 | 212 | 7.13e-30 | 110 |
| MS.gene05224.t1 | MTR_6g078250 | 40.000 | 175 | 93 | 7 | 24 | 191 | 25 | 194 | 9.36e-30 | 109 |
| MS.gene05224.t1 | MTR_6g078260 | 33.533 | 167 | 102 | 4 | 26 | 191 | 31 | 189 | 3.59e-23 | 92.4 |
| MS.gene05224.t1 | MTR_6g065460 | 35.211 | 213 | 119 | 9 | 1 | 203 | 1 | 204 | 1.17e-21 | 88.6 |
| MS.gene05224.t1 | MTR_3g106585 | 34.031 | 191 | 101 | 8 | 21 | 203 | 24 | 197 | 1.64e-21 | 88.2 |
| MS.gene05224.t1 | MTR_3g106620 | 33.333 | 189 | 103 | 9 | 23 | 203 | 26 | 199 | 2.82e-19 | 82.4 |
| MS.gene05224.t1 | MTR_0100s0150 | 31.527 | 203 | 120 | 10 | 8 | 202 | 9 | 200 | 7.67e-19 | 80.9 |
| MS.gene05224.t1 | MTR_2g072980 | 32.597 | 181 | 109 | 7 | 10 | 182 | 14 | 189 | 1.21e-18 | 80.5 |
| MS.gene05224.t1 | MTR_2g073010 | 32.367 | 207 | 116 | 10 | 5 | 203 | 8 | 198 | 1.31e-18 | 80.1 |
| MS.gene05224.t1 | MTR_0302s0030 | 32.065 | 184 | 109 | 9 | 26 | 202 | 27 | 201 | 1.55e-17 | 77.4 |
| MS.gene05224.t1 | MTR_6g059680 | 34.054 | 185 | 115 | 6 | 22 | 203 | 28 | 208 | 5.58e-17 | 76.3 |
| MS.gene05224.t1 | MTR_6g059650 | 31.720 | 186 | 118 | 7 | 20 | 202 | 22 | 201 | 1.55e-16 | 75.1 |
| MS.gene05224.t1 | MTR_3g463590 | 30.233 | 172 | 115 | 4 | 18 | 189 | 21 | 187 | 3.81e-16 | 73.9 |
| MS.gene05224.t1 | MTR_6g059730 | 32.227 | 211 | 128 | 8 | 1 | 203 | 1 | 204 | 1.13e-15 | 72.8 |
| MS.gene05224.t1 | MTR_8g461050 | 31.553 | 206 | 122 | 12 | 7 | 202 | 5 | 201 | 1.74e-15 | 72.0 |
| MS.gene05224.t1 | MTR_0211s0080 | 29.756 | 205 | 122 | 10 | 5 | 202 | 8 | 197 | 3.25e-15 | 71.2 |
| MS.gene05224.t1 | MTR_6g059530 | 30.583 | 206 | 129 | 7 | 1 | 203 | 1 | 195 | 1.27e-14 | 69.7 |
| MS.gene05224.t1 | MTR_6g059410 | 30.583 | 206 | 128 | 8 | 1 | 203 | 1 | 194 | 5.16e-14 | 68.2 |
| MS.gene05224.t1 | MTR_8g060550 | 28.866 | 194 | 119 | 10 | 6 | 189 | 4 | 188 | 6.26e-14 | 67.8 |
| MS.gene05224.t1 | MTR_8g461690 | 28.866 | 194 | 119 | 10 | 6 | 189 | 4 | 188 | 6.26e-14 | 67.8 |
| MS.gene05224.t1 | MTR_6g066210 | 29.327 | 208 | 131 | 8 | 5 | 203 | 4 | 204 | 9.50e-13 | 64.7 |
| MS.gene05224.t1 | MTR_6g059760 | 30.952 | 210 | 124 | 10 | 1 | 200 | 1 | 199 | 1.22e-12 | 64.3 |
| MS.gene05224.t1 | MTR_6g009650 | 30.435 | 207 | 128 | 9 | 5 | 203 | 4 | 202 | 1.38e-12 | 64.3 |
| MS.gene05224.t1 | MTR_6g009563 | 30.769 | 208 | 127 | 10 | 5 | 203 | 4 | 203 | 4.77e-12 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05224.t1 | AT1G17860 | 47.368 | 209 | 90 | 9 | 1 | 203 | 1 | 195 | 5.79e-53 | 168 |
| MS.gene05224.t1 | AT1G73260 | 36.464 | 181 | 107 | 5 | 26 | 203 | 30 | 205 | 1.92e-28 | 106 |
| MS.gene05224.t1 | AT1G73325 | 33.645 | 214 | 120 | 8 | 1 | 203 | 1 | 203 | 5.64e-23 | 92.4 |
Find 39 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTTGGGGTTGTGTTTAAA+AGG | 0.147873 | 6.2:-77614837 | MS.gene05224:CDS |
| ACACCTTGTTTAGCATTATA+AGG | 0.259304 | 6.2:+77615165 | None:intergenic |
| AGTGATCAAATTCCATCATT+TGG | 0.262408 | 6.2:-77614853 | MS.gene05224:CDS |
| GTGATCAAATTCCATCATTT+GGG | 0.264038 | 6.2:-77614852 | MS.gene05224:CDS |
| GTATGGCAACTGTTGGTAAT+TGG | 0.271386 | 6.2:-77615005 | MS.gene05224:CDS |
| GAACCAGTTCTTGACATTTC+AGG | 0.287884 | 6.2:-77615351 | MS.gene05224:CDS |
| GCTTCTCTTGAACAACATAA+AGG | 0.342977 | 6.2:+77615223 | None:intergenic |
| CAAACTTTGTGTAGGGAACT+AGG | 0.366708 | 6.2:-77614916 | MS.gene05224:CDS |
| CTTCTCTTGAACAACATAAA+GGG | 0.369437 | 6.2:+77615224 | None:intergenic |
| AGGGTTGTTTGTTGATGATA+AGG | 0.370874 | 6.2:-77614896 | MS.gene05224:CDS |
| AATGTCCTCAAACACAAGTT+TGG | 0.377880 | 6.2:-77615092 | MS.gene05224:CDS |
| TTACCAACAGTTGCCATACT+TGG | 0.386300 | 6.2:+77615009 | None:intergenic |
| AGCTTCCAAACTTGTGTTTG+AGG | 0.408512 | 6.2:+77615087 | None:intergenic |
| GATGGAAAATGCTAAGACTA+AGG | 0.440045 | 6.2:+77615413 | None:intergenic |
| GGGTTGTTTGTTGATGATAA+GGG | 0.441383 | 6.2:-77614895 | MS.gene05224:CDS |
| TGTAGAAGGTAATCCAAGTA+TGG | 0.452203 | 6.2:-77615022 | MS.gene05224:CDS |
| TTAAACACAACCCCAAATGA+TGG | 0.464112 | 6.2:+77614841 | None:intergenic |
| GAAATGTCAAGAACTGGTTC+AGG | 0.479615 | 6.2:+77615354 | None:intergenic |
| TGACATTTGCAAGCTTCAGC+AGG | 0.493041 | 6.2:+77614937 | None:intergenic |
| AAGCTTCTTAAAGAGTTGAC+AGG | 0.493248 | 6.2:-77615069 | MS.gene05224:CDS |
| TTACCAGTCATAAAAGGTAA+AGG | 0.497856 | 6.2:-77615294 | MS.gene05224:CDS |
| GCAAATGTCAAACTTTGTGT+AGG | 0.501531 | 6.2:-77614924 | MS.gene05224:CDS |
| TTGCCTGAAATGTCAAGAAC+TGG | 0.505604 | 6.2:+77615348 | None:intergenic |
| ATTTGGGGTTGTGTTTAAAA+GGG | 0.506662 | 6.2:-77614836 | MS.gene05224:CDS |
| CAAATGTCAAACTTTGTGTA+GGG | 0.511038 | 6.2:-77614923 | MS.gene05224:CDS |
| TCAGGCAAACAAGTAACAAC+GGG | 0.511476 | 6.2:-77615333 | MS.gene05224:CDS |
| TTAAAGAGTTGACAGGGGTG+TGG | 0.531517 | 6.2:-77615062 | MS.gene05224:CDS |
| GAGAAGCTTGAAGTAAAGAA+TGG | 0.531990 | 6.2:-77615207 | MS.gene05224:CDS |
| AAACTTTGTGTAGGGAACTA+GGG | 0.532296 | 6.2:-77614915 | MS.gene05224:CDS |
| GTCATAAAAGGTAAAGGTGG+TGG | 0.532373 | 6.2:-77615288 | MS.gene05224:CDS |
| AGCTTCTTAAAGAGTTGACA+GGG | 0.566536 | 6.2:-77615068 | MS.gene05224:CDS |
| TTCTCTTGAACAACATAAAG+GGG | 0.573118 | 6.2:+77615225 | None:intergenic |
| AATCCAAGTATGGCAACTGT+TGG | 0.585675 | 6.2:-77615012 | MS.gene05224:CDS |
| TTCAGGCAAACAAGTAACAA+CGG | 0.605095 | 6.2:-77615334 | MS.gene05224:CDS |
| CCAGTCATAAAAGGTAAAGG+TGG | 0.625936 | 6.2:-77615291 | MS.gene05224:CDS |
| TGATCAAATTCCATCATTTG+GGG | 0.645084 | 6.2:-77614851 | MS.gene05224:CDS |
| ACACCTTATAATGCTAAACA+AGG | 0.651507 | 6.2:-77615168 | MS.gene05224:CDS |
| GGTTTAACAGTTGCAAACCA+TGG | 0.676406 | 6.2:-77615267 | MS.gene05224:CDS |
| GCTTCTTAAAGAGTTGACAG+GGG | 0.746376 | 6.2:-77615067 | MS.gene05224:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACATTTAGTTTTTGTTACAA+AGG | + | chr6.2:77615144-77615163 | None:intergenic | 20.0% |
| !!! | TATATTTTACCAGTCATAAA+AGG | - | chr6.2:77614951-77614970 | MS.gene05224:CDS | 20.0% |
| AATGAAGGCTAAAAAGATGA+TGG | + | chr6.2:77614859-77614878 | None:intergenic | 30.0% | |
| ACACCTTATAATGCTAAACA+AGG | - | chr6.2:77615083-77615102 | MS.gene05224:CDS | 30.0% | |
| AGTGATCAAATTCCATCATT+TGG | - | chr6.2:77615398-77615417 | MS.gene05224:CDS | 30.0% | |
| CAAATGTCAAACTTTGTGTA+GGG | - | chr6.2:77615328-77615347 | MS.gene05224:CDS | 30.0% | |
| CTTCTCTTGAACAACATAAA+GGG | + | chr6.2:77615030-77615049 | None:intergenic | 30.0% | |
| GTGATCAAATTCCATCATTT+GGG | - | chr6.2:77615399-77615418 | MS.gene05224:CDS | 30.0% | |
| TGATCAAATTCCATCATTTG+GGG | - | chr6.2:77615400-77615419 | MS.gene05224:CDS | 30.0% | |
| TTACCAGTCATAAAAGGTAA+AGG | - | chr6.2:77614957-77614976 | MS.gene05224:CDS | 30.0% | |
| TTCTCTTGAACAACATAAAG+GGG | + | chr6.2:77615029-77615048 | None:intergenic | 30.0% | |
| ! | ACACCTTGTTTAGCATTATA+AGG | + | chr6.2:77615089-77615108 | None:intergenic | 30.0% |
| ! | ATGTTTGATTGTTTTCACCA+TGG | + | chr6.2:77615004-77615023 | None:intergenic | 30.0% |
| ! | ATTTGGGGTTGTGTTTAAAA+GGG | - | chr6.2:77615415-77615434 | MS.gene05224:CDS | 30.0% |
| !! | TGCAATAGTTTTGCAAATGA+AGG | + | chr6.2:77614874-77614893 | None:intergenic | 30.0% |
| AAACTTTGTGTAGGGAACTA+GGG | - | chr6.2:77615336-77615355 | MS.gene05224:CDS | 35.0% | |
| AAGCTTCTTAAAGAGTTGAC+AGG | - | chr6.2:77615182-77615201 | MS.gene05224:CDS | 35.0% | |
| AATGTCCTCAAACACAAGTT+TGG | - | chr6.2:77615159-77615178 | MS.gene05224:CDS | 35.0% | |
| AGCTTCTTAAAGAGTTGACA+GGG | - | chr6.2:77615183-77615202 | MS.gene05224:CDS | 35.0% | |
| GAGAAGCTTGAAGTAAAGAA+TGG | - | chr6.2:77615044-77615063 | MS.gene05224:CDS | 35.0% | |
| GATGGAAAATGCTAAGACTA+AGG | + | chr6.2:77614841-77614860 | None:intergenic | 35.0% | |
| GCAAATGTCAAACTTTGTGT+AGG | - | chr6.2:77615327-77615346 | MS.gene05224:CDS | 35.0% | |
| GCTTCTCTTGAACAACATAA+AGG | + | chr6.2:77615031-77615050 | None:intergenic | 35.0% | |
| TGTAGAAGGTAATCCAAGTA+TGG | - | chr6.2:77615229-77615248 | MS.gene05224:CDS | 35.0% | |
| TTAAACACAACCCCAAATGA+TGG | + | chr6.2:77615413-77615432 | None:intergenic | 35.0% | |
| TTCAGGCAAACAAGTAACAA+CGG | - | chr6.2:77614917-77614936 | MS.gene05224:CDS | 35.0% | |
| ! | CATTTGGGGTTGTGTTTAAA+AGG | - | chr6.2:77615414-77615433 | MS.gene05224:CDS | 35.0% |
| !! | AGGGTTGTTTGTTGATGATA+AGG | - | chr6.2:77615355-77615374 | MS.gene05224:CDS | 35.0% |
| !! | GGGTTGTTTGTTGATGATAA+GGG | - | chr6.2:77615356-77615375 | MS.gene05224:CDS | 35.0% |
| AATCCAAGTATGGCAACTGT+TGG | - | chr6.2:77615239-77615258 | MS.gene05224:CDS | 40.0% | |
| AGCTTCCAAACTTGTGTTTG+AGG | + | chr6.2:77615167-77615186 | None:intergenic | 40.0% | |
| CAAACTTTGTGTAGGGAACT+AGG | - | chr6.2:77615335-77615354 | MS.gene05224:CDS | 40.0% | |
| CCAGTCATAAAAGGTAAAGG+TGG | - | chr6.2:77614960-77614979 | MS.gene05224:CDS | 40.0% | |
| GAACCAGTTCTTGACATTTC+AGG | - | chr6.2:77614900-77614919 | MS.gene05224:CDS | 40.0% | |
| GCTTCTTAAAGAGTTGACAG+GGG | - | chr6.2:77615184-77615203 | MS.gene05224:CDS | 40.0% | |
| GGTTTAACAGTTGCAAACCA+TGG | - | chr6.2:77614984-77615003 | MS.gene05224:CDS | 40.0% | |
| GTCATAAAAGGTAAAGGTGG+TGG | - | chr6.2:77614963-77614982 | MS.gene05224:CDS | 40.0% | |
| TCAGGCAAACAAGTAACAAC+GGG | - | chr6.2:77614918-77614937 | MS.gene05224:CDS | 40.0% | |
| TTACCAACAGTTGCCATACT+TGG | + | chr6.2:77615245-77615264 | None:intergenic | 40.0% | |
| TTGCCTGAAATGTCAAGAAC+TGG | + | chr6.2:77614906-77614925 | None:intergenic | 40.0% | |
| ! | CCACCTTTACCTTTTATGAC+TGG | + | chr6.2:77614963-77614982 | None:intergenic | 40.0% |
| ! | GAAATGTCAAGAACTGGTTC+AGG | + | chr6.2:77614900-77614919 | None:intergenic | 40.0% |
| !! | GTATGGCAACTGTTGGTAAT+TGG | - | chr6.2:77615246-77615265 | MS.gene05224:CDS | 40.0% |
| TGACATTTGCAAGCTTCAGC+AGG | + | chr6.2:77615317-77615336 | None:intergenic | 45.0% | |
| TTAAAGAGTTGACAGGGGTG+TGG | - | chr6.2:77615189-77615208 | MS.gene05224:CDS | 45.0% | |
| !!! | GGGTGTGGTTTTTAGCTACA+GGG | - | chr6.2:77615204-77615223 | MS.gene05224:CDS | 45.0% |
| !!! | GGTGTGGTTTTTAGCTACAG+GGG | - | chr6.2:77615205-77615224 | MS.gene05224:CDS | 45.0% |
| !!! | GTGTGGTTTTTAGCTACAGG+GGG | - | chr6.2:77615206-77615225 | MS.gene05224:CDS | 45.0% |
| ! | TTAGCTACAGGGGGTGTAGA+AGG | - | chr6.2:77615215-77615234 | MS.gene05224:CDS | 50.0% |
| !!! | GGGGTGTGGTTTTTAGCTAC+AGG | - | chr6.2:77615203-77615222 | MS.gene05224:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 77614831 | 77615442 | 77614831 | ID=MS.gene05224 |
| chr6.2 | mRNA | 77614831 | 77615442 | 77614831 | ID=MS.gene05224.t1;Parent=MS.gene05224 |
| chr6.2 | exon | 77614831 | 77615442 | 77614831 | ID=MS.gene05224.t1.exon1;Parent=MS.gene05224.t1 |
| chr6.2 | CDS | 77614831 | 77615442 | 77614831 | ID=cds.MS.gene05224.t1;Parent=MS.gene05224.t1 |
| Gene Sequence |
| Protein sequence |
