Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37802.t1 | KEH26836.1 | 92.6 | 122 | 9 | 0 | 25 | 146 | 327 | 448 | 1.10E-55 | 226.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37802.t1 | Q9M1S9 | 40.0 | 85 | 51 | 0 | 54 | 138 | 390 | 474 | 1.4e-11 | 70.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37802.t1 | A0A072UAU7 | 92.6 | 122 | 9 | 0 | 25 | 146 | 409 | 530 | 7.7e-56 | 226.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050026 | MS.gene37802 | 0.813887 | 2.04E-51 | -1.69E-46 |
| MS.gene05265 | MS.gene37802 | 0.800137 | 1.66E-48 | -1.69E-46 |
| MS.gene056050 | MS.gene37802 | 0.803973 | 2.70E-49 | -1.69E-46 |
| MS.gene056854 | MS.gene37802 | 0.818252 | 2.16E-52 | -1.69E-46 |
| MS.gene059272 | MS.gene37802 | 0.845106 | 4.99E-59 | -1.69E-46 |
| MS.gene059625 | MS.gene37802 | 0.80403 | 2.63E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37802.t1 | MTR_6g477680 | 92.623 | 122 | 9 | 0 | 25 | 146 | 327 | 448 | 2.68e-76 | 233 |
| MS.gene37802.t1 | MTR_6g077680 | 92.623 | 122 | 9 | 0 | 25 | 146 | 409 | 530 | 3.03e-75 | 233 |
| MS.gene37802.t1 | MTR_6g077630 | 85.714 | 126 | 16 | 1 | 23 | 146 | 39 | 164 | 5.14e-73 | 216 |
| MS.gene37802.t1 | MTR_6g079130 | 85.714 | 126 | 16 | 1 | 23 | 146 | 91 | 216 | 6.81e-72 | 215 |
| MS.gene37802.t1 | MTR_6g077680 | 94.175 | 103 | 6 | 0 | 25 | 127 | 409 | 511 | 2.04e-62 | 202 |
| MS.gene37802.t1 | MTR_6g077630 | 87.500 | 104 | 11 | 1 | 23 | 124 | 39 | 142 | 5.42e-60 | 182 |
| MS.gene37802.t1 | MTR_6g078940 | 57.258 | 124 | 26 | 1 | 23 | 146 | 27 | 123 | 2.53e-39 | 129 |
| MS.gene37802.t1 | MTR_6g078920 | 87.324 | 71 | 8 | 1 | 23 | 93 | 133 | 202 | 6.76e-36 | 123 |
| MS.gene37802.t1 | MTR_6g078990 | 88.462 | 52 | 6 | 0 | 26 | 77 | 193 | 244 | 3.22e-25 | 96.7 |
| MS.gene37802.t1 | MTR_6g078950 | 80.952 | 42 | 8 | 0 | 95 | 136 | 66 | 107 | 5.74e-17 | 76.3 |
| MS.gene37802.t1 | MTR_1g070170 | 41.860 | 86 | 50 | 0 | 53 | 138 | 516 | 601 | 9.57e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37802.t1 | AT3G54710 | 40.476 | 84 | 50 | 0 | 55 | 138 | 391 | 474 | 5.21e-15 | 71.2 |
| MS.gene37802.t1 | AT2G31270 | 39.286 | 84 | 51 | 0 | 55 | 138 | 475 | 558 | 3.62e-14 | 68.9 |
Find 18 sgRNAs with CRISPR-Local
Find 101 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAGAACAAATTGAGATTT+TGG | 0.155684 | 6.3:-73232565 | MS.gene37802:CDS |
| TGCAAGAAGGTGCTTTCTTC+TGG | 0.218302 | 6.3:-73232519 | MS.gene37802:CDS |
| AATGTGATTGACTCTATATT+TGG | 0.242735 | 6.3:-73232758 | MS.gene37802:CDS |
| TGCAAGACTGGATCTGAAAA+AGG | 0.288868 | 6.3:-73234229 | MS.gene37802:intron |
| CAATGTAGATCAACATTGTT+TGG | 0.317145 | 6.3:-73232822 | MS.gene37802:CDS |
| TGTCAAGAATGTGTTAGATT+TGG | 0.348709 | 6.3:-73232158 | MS.gene37802:CDS |
| ACCTTCTTGCAAAGCCAGTC+TGG | 0.406749 | 6.3:+73232531 | None:intergenic |
| TCGAGAAGTTTGTGCAAGAC+TGG | 0.426946 | 6.3:-73234241 | MS.gene37802:CDS |
| TGGAAAAGACTGTGCCAGAC+TGG | 0.439177 | 6.3:-73232545 | MS.gene37802:CDS |
| ATCAGTTCCACCAATGATGA+AGG | 0.461776 | 6.3:+73232782 | None:intergenic |
| GGATAACAATCCTTCATCAT+TGG | 0.485163 | 6.3:-73232792 | MS.gene37802:CDS |
| TTCAAAATATTTGCAACAAG+AGG | 0.503578 | 6.3:-73233958 | MS.gene37802:intron |
| AAGAACTTCAATCACAAAAG+AGG | 0.504889 | 6.3:-73232726 | MS.gene37802:CDS |
| CTTCTGGAGACACTATGTAC+TGG | 0.511897 | 6.3:-73232503 | MS.gene37802:intron |
| TCAACATTGTTTGGATGCAT+CGG | 0.523522 | 6.3:-73232813 | MS.gene37802:CDS |
| TAACAATCCTTCATCATTGG+TGG | 0.549598 | 6.3:-73232789 | MS.gene37802:CDS |
| GCCAGACTGGCTTTGCAAGA+AGG | 0.550066 | 6.3:-73232532 | MS.gene37802:CDS |
| CTTCTCAGCAATGCGAACAA+AGG | 0.588099 | 6.3:-73232118 | MS.gene37802:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAAATCAATTAAATTTG+AGG | + | chr6.3:73232451-73232470 | None:intergenic | 10.0% |
| !! | AATAAATCAATTAAATTTGA+GGG | + | chr6.3:73232450-73232469 | None:intergenic | 10.0% |
| !! | TTGAAAAATATATAAATATG+AGG | + | chr6.3:73232253-73232272 | None:intergenic | 10.0% |
| !!! | TCATATTTATATATTTTTCA+AGG | - | chr6.3:73232252-73232271 | MS.gene37802:intron | 10.0% |
| !! | TTTAACAATAAAGAGAATTA+AGG | + | chr6.3:73233426-73233445 | None:intergenic | 15.0% |
| !!! | ATAATTTCCTATTTTAATAC+AGG | - | chr6.3:73232301-73232320 | MS.gene37802:intron | 15.0% |
| !!! | ATCATAAGTCTTTTTTTTTA+AGG | - | chr6.3:73232758-73232777 | MS.gene37802:CDS | 15.0% |
| !! | AAAAAAAGACTTATGATAGA+AGG | + | chr6.3:73232755-73232774 | None:intergenic | 20.0% |
| !! | ATCAAAATACAACCATAAAA+TGG | + | chr6.3:73232485-73232504 | None:intergenic | 20.0% |
| !! | CCAAAATAATGAAATTACTT+TGG | + | chr6.3:73233311-73233330 | None:intergenic | 20.0% |
| !! | TTATAATGTACACAATTTCT+TGG | - | chr6.3:73233726-73233745 | MS.gene37802:intron | 20.0% |
| !!! | AATAGGAAATTATACTTTTC+TGG | + | chr6.3:73232294-73232313 | None:intergenic | 20.0% |
| !!! | CCAAAGTAATTTCATTATTT+TGG | - | chr6.3:73233308-73233327 | MS.gene37802:intron | 20.0% |
| !!! | GTAATTGATTTCTTGTTAAT+TGG | - | chr6.3:73233468-73233487 | MS.gene37802:intron | 20.0% |
| !!! | TATAATGGAATAATAGAACT+TGG | - | chr6.3:73234036-73234055 | MS.gene37802:intron | 20.0% |
| !!! | TCATAATTTTACATATGTCA+TGG | - | chr6.3:73234012-73234031 | MS.gene37802:intron | 20.0% |
| ! | AACTTCTCATATAATGTCAA+CGG | - | chr6.3:73233020-73233039 | MS.gene37802:intron | 25.0% |
| ! | AATCAATGAATCAAAGGAAA+TGG | + | chr6.3:73232937-73232956 | None:intergenic | 25.0% |
| ! | ATATTGGATGTCAAGAATAT+GGG | + | chr6.3:73233364-73233383 | None:intergenic | 25.0% |
| ! | ATCAATGAATCAAAGGAAAT+GGG | + | chr6.3:73232936-73232955 | None:intergenic | 25.0% |
| ! | ATTAAATTTCAGCCTTCTAT+AGG | - | chr6.3:73233979-73233998 | MS.gene37802:intron | 25.0% |
| ! | TACATATGTCATGGATATAA+TGG | - | chr6.3:73234021-73234040 | MS.gene37802:intron | 25.0% |
| ! | TCTTGAACCTGTATTAAAAT+AGG | + | chr6.3:73232311-73232330 | None:intergenic | 25.0% |
| ! | TTCAAAATATTTGCAACAAG+AGG | - | chr6.3:73232403-73232422 | MS.gene37802:intron | 25.0% |
| ! | TTCCTTAATCAATGAATCAA+AGG | + | chr6.3:73232943-73232962 | None:intergenic | 25.0% |
| !! | AATGTGATTGACTCTATATT+TGG | - | chr6.3:73233603-73233622 | MS.gene37802:intron | 25.0% |
| !! | GATTTTTAATAGCCTATAGA+AGG | + | chr6.3:73233994-73234013 | None:intergenic | 25.0% |
| !! | TGAAGAACAAATTGAGATTT+TGG | - | chr6.3:73233796-73233815 | MS.gene37802:intron | 25.0% |
| !! | TTCCTTTGATTCATTGATTA+AGG | - | chr6.3:73232938-73232957 | MS.gene37802:intron | 25.0% |
| !!! | GAAATGTGTCTTTTAAAAGA+TGG | + | chr6.3:73233102-73233121 | None:intergenic | 25.0% |
| !!! | GGTTTGTTAAAATTCTACTA+CGG | + | chr6.3:73232838-73232857 | None:intergenic | 25.0% |
| !!! | TTTTCTGAAAAGAATGACTA+AGG | + | chr6.3:73233518-73233537 | None:intergenic | 25.0% |
| AAGAACTTCAATCACAAAAG+AGG | - | chr6.3:73233635-73233654 | MS.gene37802:intron | 30.0% | |
| ACATCTTCTATCATAAGTGT+TGG | - | chr6.3:73232627-73232646 | MS.gene37802:intron | 30.0% | |
| ATACACTTGAATCAATGCTA+TGG | - | chr6.3:73233199-73233218 | MS.gene37802:intron | 30.0% | |
| ATCACTAATCAAATCAGCAA+TGG | + | chr6.3:73232334-73232353 | None:intergenic | 30.0% | |
| CAATGTAGATCAACATTGTT+TGG | - | chr6.3:73233539-73233558 | MS.gene37802:intron | 30.0% | |
| GATATTGGATGTCAAGAATA+TGG | + | chr6.3:73233365-73233384 | None:intergenic | 30.0% | |
| GATCATAACCTTTGACAAAT+GGG | + | chr6.3:73233177-73233196 | None:intergenic | 30.0% | |
| GATCATTCTATCATAAGTGT+TGG | - | chr6.3:73232780-73232799 | MS.gene37802:CDS | 30.0% | |
| TGAAAAAGGTTAGATTCTAC+TGG | - | chr6.3:73232146-73232165 | MS.gene37802:CDS | 30.0% | |
| TGATCATAACCTTTGACAAA+TGG | + | chr6.3:73233178-73233197 | None:intergenic | 30.0% | |
| TGTAAGTTGTAACTCTTCAT+GGG | + | chr6.3:73233258-73233277 | None:intergenic | 30.0% | |
| TGTCAAGAATGTGTTAGATT+TGG | - | chr6.3:73234203-73234222 | MS.gene37802:intron | 30.0% | |
| TTGTAAGTTGTAACTCTTCA+TGG | + | chr6.3:73233259-73233278 | None:intergenic | 30.0% | |
| ! | AGCAGAACTTTTGATGTTAT+TGG | - | chr6.3:73234073-73234092 | MS.gene37802:intron | 30.0% |
| ! | GGAAATTATACTTTTCTGGA+AGG | + | chr6.3:73232290-73232309 | None:intergenic | 30.0% |
| ! | TATGGGTACTAACCATTTTA+TGG | - | chr6.3:73232470-73232489 | MS.gene37802:intron | 30.0% |
| !! | AATTATACTTTTCTGGAAGG+TGG | + | chr6.3:73232287-73232306 | None:intergenic | 30.0% |
| !! | ATTATACTTTTCTGGAAGGT+GGG | + | chr6.3:73232286-73232305 | None:intergenic | 30.0% |
| AATAGAACTTGGCAGTTTGA+TGG | - | chr6.3:73234047-73234066 | MS.gene37802:intron | 35.0% | |
| CTTGACACTGCAAATATAAC+AGG | + | chr6.3:73234190-73234209 | None:intergenic | 35.0% | |
| GAGGATCAAAATCTTGATTG+AGG | + | chr6.3:73232234-73232253 | None:intergenic | 35.0% | |
| GAGTTACAACTTACAACTCT+TGG | - | chr6.3:73233263-73233282 | MS.gene37802:intron | 35.0% | |
| GGAAATATACTATATCCTGC+AGG | - | chr6.3:73232167-73232186 | MS.gene37802:intron | 35.0% | |
| GGATAACAATCCTTCATCAT+TGG | - | chr6.3:73233569-73233588 | MS.gene37802:intron | 35.0% | |
| TAACAATCCTTCATCATTGG+TGG | - | chr6.3:73233572-73233591 | MS.gene37802:intron | 35.0% | |
| TCAACATTGTTTGGATGCAT+CGG | - | chr6.3:73233548-73233567 | MS.gene37802:intron | 35.0% | |
| ! | CTGCTACTCTAAAAGAACAA+AGG | + | chr6.3:73233921-73233940 | None:intergenic | 35.0% |
| ! | TGGTGAAGAGTTACAACTTT+TGG | - | chr6.3:73233283-73233302 | MS.gene37802:intron | 35.0% |
| !! | TGCAAAGTGTTGTCAAATAG+AGG | - | chr6.3:73232867-73232886 | MS.gene37802:intron | 35.0% |
| !!! | TTTTAAAACAGATCACCTGC+AGG | + | chr6.3:73232185-73232204 | None:intergenic | 35.0% |
| ACACATTCTATCACAGGTGT+TGG | - | chr6.3:73232686-73232705 | MS.gene37802:CDS | 40.0% | |
| ACTAATCTTGTCCTCCCAAT+AGG | - | chr6.3:73233767-73233786 | MS.gene37802:intron | 40.0% | |
| ATCAAGCACCCATTTGTCAA+AGG | - | chr6.3:73233166-73233185 | MS.gene37802:intron | 40.0% | |
| ATCAGTTCCACCAATGATGA+AGG | + | chr6.3:73233582-73233601 | None:intergenic | 40.0% | |
| CAAATAGAGGCTAACGCTAA+AGG | - | chr6.3:73232880-73232899 | MS.gene37802:intron | 40.0% | |
| CAACACTTTGCACTAAATCG+TGG | + | chr6.3:73232859-73232878 | None:intergenic | 40.0% | |
| CAGCGACAGTTCAATTTCAT+TGG | + | chr6.3:73233061-73233080 | None:intergenic | 40.0% | |
| GTTCTTCAGCTTCTCCTATT+GGG | + | chr6.3:73233784-73233803 | None:intergenic | 40.0% | |
| TGCAAGACTGGATCTGAAAA+AGG | - | chr6.3:73232132-73232151 | MS.gene37802:CDS | 40.0% | |
| TGTTATGAGCCAAAATCCTC+AGG | - | chr6.3:73234103-73234122 | MS.gene37802:intron | 40.0% | |
| TGTTCTTCAGCTTCTCCTAT+TGG | + | chr6.3:73233785-73233804 | None:intergenic | 40.0% | |
| TGTTGATCAAATGCGAAGTG+TGG | + | chr6.3:73233396-73233415 | None:intergenic | 40.0% | |
| TTGAGCACACATTCTATCAC+AGG | - | chr6.3:73232680-73232699 | MS.gene37802:CDS | 40.0% | |
| ! | AAGTAAACGCCTGAGGATTT+TGG | + | chr6.3:73234115-73234134 | None:intergenic | 40.0% |
| ! | GTTAATTGGTTTCTCGATGC+AGG | - | chr6.3:73233482-73233501 | MS.gene37802:intron | 40.0% |
| ! | TAGTTTGAAGTAAACGCCTG+AGG | + | chr6.3:73234122-73234141 | None:intergenic | 40.0% |
| AAATGCGAAGTGTGGAAGGA+AGG | + | chr6.3:73233388-73233407 | None:intergenic | 45.0% | |
| AATGCGAAGTGTGGAAGGAA+GGG | + | chr6.3:73233387-73233406 | None:intergenic | 45.0% | |
| AGTGTGGAAGGAAGGGATAT+TGG | + | chr6.3:73233380-73233399 | None:intergenic | 45.0% | |
| CTTCTCAGCAATGCGAACAA+AGG | - | chr6.3:73234243-73234262 | MS.gene37802:CDS | 45.0% | |
| GATCAAATGCGAAGTGTGGA+AGG | + | chr6.3:73233392-73233411 | None:intergenic | 45.0% | |
| TCGAGAAGTTTGTGCAAGAC+TGG | - | chr6.3:73232120-73232139 | MS.gene37802:CDS | 45.0% | |
| ! | CTTCTGGAGACACTATGTAC+TGG | - | chr6.3:73233858-73233877 | MS.gene37802:intron | 45.0% |
| ! | TGGAGACACTATGTACTGGT+GGG | - | chr6.3:73233862-73233881 | MS.gene37802:intron | 45.0% |
| !! | TGCAAGAAGGTGCTTTCTTC+TGG | - | chr6.3:73233842-73233861 | MS.gene37802:intron | 45.0% |
| !! | TTTTCTGGAAGGTGGGTTTG+TGG | + | chr6.3:73232279-73232298 | None:intergenic | 45.0% |
| !!! | AAATTTAATTGATTTATTTA+TGG | - | chr6.3:73232452-73232471 | MS.gene37802:intron | 5.0% |
| !!! | AATTTAATTGATTTATTTAT+GGG | - | chr6.3:73232453-73232472 | MS.gene37802:intron | 5.0% |
| !!! | ATTTTTTTTCAAATAATTTT+CGG | + | chr6.3:73232587-73232606 | None:intergenic | 5.0% |
| ACCTTCTTGCAAAGCCAGTC+TGG | + | chr6.3:73233833-73233852 | None:intergenic | 50.0% | |
| AGAGTTGTGTGCGTGTGTGT+CGG | - | chr6.3:73232654-73232673 | MS.gene37802:intron | 50.0% | |
| CTGGAGACACTATGTACTGG+TGG | - | chr6.3:73233861-73233880 | MS.gene37802:intron | 50.0% | |
| CTTCAGCTTCTCCTATTGGG+AGG | + | chr6.3:73233781-73233800 | None:intergenic | 50.0% | |
| TGGAAAAGACTGTGCCAGAC+TGG | - | chr6.3:73233816-73233835 | MS.gene37802:intron | 50.0% | |
| !! | ACAGGTGTTGGTGCTACAGT+GGG | - | chr6.3:73232698-73232717 | MS.gene37802:CDS | 50.0% |
| TGGGGTGTGTGTGTGTGTGT+CGG | - | chr6.3:73232717-73232736 | MS.gene37802:CDS | 55.0% | |
| !! | CACAGGTGTTGGTGCTACAG+TGG | - | chr6.3:73232697-73232716 | MS.gene37802:CDS | 55.0% |
| !! | CAGGTGTTGGTGCTACAGTG+GGG | - | chr6.3:73232699-73232718 | MS.gene37802:CDS | 55.0% |
| !! | GCCAGACTGGCTTTGCAAGA+AGG | - | chr6.3:73233829-73233848 | MS.gene37802:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 73232108 | 73234275 | 73232108 | ID=MS.gene37802 |
| chr6.3 | mRNA | 73232108 | 73234275 | 73232108 | ID=MS.gene37802.t1;Parent=MS.gene37802 |
| chr6.3 | exon | 73234230 | 73234275 | 73234230 | ID=MS.gene37802.t1.exon1;Parent=MS.gene37802.t1 |
| chr6.3 | CDS | 73234230 | 73234275 | 73234230 | ID=cds.MS.gene37802.t1;Parent=MS.gene37802.t1 |
| chr6.3 | exon | 73233959 | 73233990 | 73233959 | ID=MS.gene37802.t1.exon2;Parent=MS.gene37802.t1 |
| chr6.3 | CDS | 73233959 | 73233990 | 73233959 | ID=cds.MS.gene37802.t1;Parent=MS.gene37802.t1 |
| chr6.3 | exon | 73232678 | 73232879 | 73232678 | ID=MS.gene37802.t1.exon3;Parent=MS.gene37802.t1 |
| chr6.3 | CDS | 73232678 | 73232879 | 73232678 | ID=cds.MS.gene37802.t1;Parent=MS.gene37802.t1 |
| chr6.3 | exon | 73232504 | 73232594 | 73232504 | ID=MS.gene37802.t1.exon4;Parent=MS.gene37802.t1 |
| chr6.3 | CDS | 73232504 | 73232594 | 73232504 | ID=cds.MS.gene37802.t1;Parent=MS.gene37802.t1 |
| chr6.3 | exon | 73232108 | 73232180 | 73232108 | ID=MS.gene37802.t1.exon5;Parent=MS.gene37802.t1 |
| chr6.3 | CDS | 73232108 | 73232180 | 73232108 | ID=cds.MS.gene37802.t1;Parent=MS.gene37802.t1 |
| Gene Sequence |
| Protein sequence |
