Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38167.t1 | XP_003618014.1 | 96.4 | 56 | 2 | 0 | 1 | 56 | 1 | 56 | 1.10E-15 | 93.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38167.t1 | G7K500 | 96.4 | 56 | 2 | 0 | 1 | 56 | 1 | 56 | 7.9e-16 | 93.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050648 | MS.gene38167 | 0.80049 | 1.41E-48 | -1.69E-46 |
| MS.gene051648 | MS.gene38167 | 0.818594 | 1.81E-52 | -1.69E-46 |
| MS.gene052150 | MS.gene38167 | 0.816748 | 4.71E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38167.t1 | MTR_5g098020 | 90.256 | 195 | 19 | 0 | 1 | 195 | 1 | 195 | 9.05e-79 | 233 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 37 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAGCTGAAGATTCTTCTT+TGG | 0.248746 | 5.3:+79264603 | None:intergenic |
| TTCTGTTGTTGAGGCTGTTC+AGG | 0.288375 | 5.3:-79264401 | MS.gene38167:CDS |
| TCTGATGCTGCTGGTGTGAT+TGG | 0.302513 | 5.3:+79264628 | None:intergenic |
| GTCGAGGAAACAAAAGAAAA+TGG | 0.336894 | 5.3:-79264427 | MS.gene38167:CDS |
| CTCAACAGGTTCCTTTGTCT+CGG | 0.374352 | 5.3:+79264480 | None:intergenic |
| TCTGTCTCAGATTCTGCAGC+TGG | 0.400444 | 5.3:+79264373 | None:intergenic |
| TGGACTCTCAATGATTTCTT+CGG | 0.404920 | 5.3:+79264648 | None:intergenic |
| CTCAGCTTTAAGCAAAGGTT+TGG | 0.421527 | 5.3:+79264576 | None:intergenic |
| CCTTACTCACTCTTCTCAGT+TGG | 0.426357 | 5.3:+79264166 | None:intergenic |
| TGAGACAGAAAATGTTGTTA+AGG | 0.433126 | 5.3:-79264359 | MS.gene38167:CDS |
| GGTAGCTGAGGCTGAAACTA+AGG | 0.438659 | 5.3:-79264326 | MS.gene38167:CDS |
| CCAACTGAGAAGAGTGAGTA+AGG | 0.442677 | 5.3:-79264166 | None:intergenic |
| GGTTGAGAAAGAAGAAGCCT+TGG | 0.446199 | 5.3:-79264257 | MS.gene38167:CDS |
| TCTTTGGTTTCTGATGCTGC+TGG | 0.462673 | 5.3:+79264619 | None:intergenic |
| GCTTAAAGCTGAGGCAAAAG+AGG | 0.486761 | 5.3:-79264566 | MS.gene38167:CDS |
| TCCAATTATGGCCACTGTTG+AGG | 0.502667 | 5.3:-79265445 | None:intergenic |
| TACAGCAGAGGAAACAAAGA+AGG | 0.515632 | 5.3:-79264512 | MS.gene38167:CDS |
| AATTGAGAAGAGTGAAGAAG+AGG | 0.516526 | 5.3:-79264191 | MS.gene38167:CDS |
| ACTCTCAATGATTTCTTCGG+TGG | 0.520132 | 5.3:+79264651 | None:intergenic |
| GGAAGAACAAACCGAGACAA+AGG | 0.535864 | 5.3:-79264491 | MS.gene38167:CDS |
| AGAAGAGAAAAGTGAGGAGA+AGG | 0.537941 | 5.3:-79264278 | MS.gene38167:CDS |
| GTTGAGGAAACAAAAGAAAA+CGG | 0.538839 | 5.3:-79264463 | MS.gene38167:CDS |
| TTTGCCTCAGCTTTAAGCAA+AGG | 0.549317 | 5.3:+79264571 | None:intergenic |
| AGCCTTGGATGAAAAGAAAG+AGG | 0.562181 | 5.3:-79264242 | MS.gene38167:CDS |
| CAAACCTTTGCTTAAAGCTG+AGG | 0.572895 | 5.3:-79264575 | MS.gene38167:CDS |
| CGAGACAAAGGAACCTGTTG+AGG | 0.574462 | 5.3:-79264479 | MS.gene38167:CDS |
| AAATGGTGATTCTGTTGTTG+AGG | 0.613322 | 5.3:-79264410 | MS.gene38167:CDS |
| TGTTGTTAAGGATGAGAATG+TGG | 0.621253 | 5.3:-79264347 | MS.gene38167:CDS |
| TGATAACTGAGCAGTGTCGT+CGG | 0.622323 | 5.3:+79264693 | None:intergenic |
| CGGTGATTCAGTTAATGTCG+AGG | 0.622446 | 5.3:-79264443 | MS.gene38167:CDS |
| AGCCACAGAAGAGAAAAGTG+AGG | 0.638283 | 5.3:-79264284 | MS.gene38167:CDS |
| CAACTGAGAAGAGTGAGTAA+GGG | 0.654993 | 5.3:-79264165 | None:intergenic |
| GGATGAGAATGTGGTAGCTG+AGG | 0.670593 | 5.3:-79264338 | MS.gene38167:CDS |
| TAAAGCTGAGGCAAAAGAGG+TGG | 0.674001 | 5.3:-79264563 | MS.gene38167:CDS |
| GGTGGTAGAAGAAGTTCTTG+TGG | 0.675484 | 5.3:-79264545 | MS.gene38167:CDS |
| GGCTGTGAAAGATACAGCAG+AGG | 0.684792 | 5.3:-79264524 | MS.gene38167:CDS |
| TGGTAGAGTTACCTCAACAG+TGG | 0.712754 | 5.3:+79265434 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGGAAATATTAATTATAAT+TGG | - | chr5.3:79264549-79264568 | MS.gene38167:CDS | 10.0% |
| !!! | AATCAATGTTTAAAATCAAA+TGG | + | chr5.3:79264244-79264263 | None:intergenic | 15.0% |
| !!! | AATTTTGAGTCTTTTATTTT+TGG | - | chr5.3:79264655-79264674 | MS.gene38167:CDS | 15.0% |
| !! | AAAAATTGAAGCTCTTAAAA+TGG | - | chr5.3:79264530-79264549 | MS.gene38167:CDS | 20.0% |
| !! | AATCAAATTGTGATTCATAT+TGG | - | chr5.3:79264367-79264386 | MS.gene38167:CDS | 20.0% |
| !! | AGATAATTTATCCTTATGAA+CGG | + | chr5.3:79264693-79264712 | None:intergenic | 20.0% |
| !!! | AATATTTTAAAGCATCTAGT+AGG | - | chr5.3:79264278-79264297 | MS.gene38167:CDS | 20.0% |
| !!! | GAACAAAATTTGTTTTAGAT+AGG | - | chr5.3:79264748-79264767 | MS.gene38167:intron | 20.0% |
| !!! | TTTACTTTTTAATCGAACTT+TGG | + | chr5.3:79264724-79264743 | None:intergenic | 20.0% |
| ! | AAAAACCAAGTACTTGAAAT+TGG | + | chr5.3:79264195-79264214 | None:intergenic | 25.0% |
| ! | GATAATTTATCCTTATGAAC+GGG | + | chr5.3:79264692-79264711 | None:intergenic | 25.0% |
| ! | TCATATCTTCTTGAGTAAAT+AGG | + | chr5.3:79264503-79264522 | None:intergenic | 25.0% |
| ! | TTTCAATACTCTGACTAATA+TGG | + | chr5.3:79264627-79264646 | None:intergenic | 25.0% |
| !! | CAAATGTTGTTTATTAGACA+AGG | + | chr5.3:79264841-79264860 | None:intergenic | 25.0% |
| !!! | ACCTTTTTTAATGCATTAGA+TGG | - | chr5.3:79264468-79264487 | MS.gene38167:CDS | 25.0% |
| !!! | TCAATATTTTAATGTCTCTC+CGG | + | chr5.3:79264446-79264465 | None:intergenic | 25.0% |
| ATCTTCTTGAGTAAATAGGA+AGG | + | chr5.3:79264499-79264518 | None:intergenic | 30.0% | |
| ATGTGCATGATTAATTCATG+TGG | + | chr5.3:79264593-79264612 | None:intergenic | 30.0% | |
| GCCATCTAATGCATTAAAAA+AGG | + | chr5.3:79264472-79264491 | None:intergenic | 30.0% | |
| GTTGAGGAAACAAAAGAAAA+CGG | - | chr5.3:79265143-79265162 | MS.gene38167:intron | 30.0% | |
| TGAGACAGAAAATGTTGTTA+AGG | - | chr5.3:79265247-79265266 | MS.gene38167:intron | 30.0% | |
| ! | CTCAATTTTTGCATCATTGT+TGG | + | chr5.3:79265402-79265421 | None:intergenic | 30.0% |
| !! | TTTTCTTTTGTTTCCTCAAC+AGG | + | chr5.3:79265143-79265162 | None:intergenic | 30.0% |
| !!! | ACATGACATGTTTTAATCAC+GGG | - | chr5.3:79264341-79264360 | MS.gene38167:CDS | 30.0% |
| !!! | CACATGACATGTTTTAATCA+CGG | - | chr5.3:79264340-79264359 | MS.gene38167:CDS | 30.0% |
| AAATGGTGATTCTGTTGTTG+AGG | - | chr5.3:79265196-79265215 | MS.gene38167:intron | 35.0% | |
| AATTGAGAAGAGTGAAGAAG+AGG | - | chr5.3:79265415-79265434 | MS.gene38167:intron | 35.0% | |
| AATTTATCCTTATGAACGGG+AGG | + | chr5.3:79264689-79264708 | None:intergenic | 35.0% | |
| ATTTATCCTTATGAACGGGA+GGG | + | chr5.3:79264688-79264707 | None:intergenic | 35.0% | |
| CGTGATTAAAACATGTCATG+TGG | + | chr5.3:79264342-79264361 | None:intergenic | 35.0% | |
| GTCGAGGAAACAAAAGAAAA+TGG | - | chr5.3:79265179-79265198 | MS.gene38167:intron | 35.0% | |
| TGATCTGAATATGATTCTGC+AGG | - | chr5.3:79264866-79264885 | MS.gene38167:intron | 35.0% | |
| TGGACTCTCAATGATTTCTT+CGG | + | chr5.3:79264961-79264980 | None:intergenic | 35.0% | |
| TGTTGTTAAGGATGAGAATG+TGG | - | chr5.3:79265259-79265278 | MS.gene38167:intron | 35.0% | |
| TTCAGCTGAAGATTCTTCTT+TGG | + | chr5.3:79265006-79265025 | None:intergenic | 35.0% | |
| ! | CTCTACCAATTTCAAGTACT+TGG | - | chr5.3:79264187-79264206 | MS.gene38167:CDS | 35.0% |
| ! | TTCCTCTTTCTTTTCATCCA+AGG | + | chr5.3:79265369-79265388 | None:intergenic | 35.0% |
| ACTCTCAATGATTTCTTCGG+TGG | + | chr5.3:79264958-79264977 | None:intergenic | 40.0% | |
| AGAAGAGAAAAGTGAGGAGA+AGG | - | chr5.3:79265328-79265347 | MS.gene38167:intron | 40.0% | |
| AGCCTTGGATGAAAAGAAAG+AGG | - | chr5.3:79265364-79265383 | MS.gene38167:intron | 40.0% | |
| ATGTCATGTGGAAGTAATGC+AGG | + | chr5.3:79264330-79264349 | None:intergenic | 40.0% | |
| CAAACCTTTGCTTAAAGCTG+AGG | - | chr5.3:79265031-79265050 | MS.gene38167:intron | 40.0% | |
| CTCTGACTAATATGGACACA+TGG | + | chr5.3:79264619-79264638 | None:intergenic | 40.0% | |
| TACAGCAGAGGAAACAAAGA+AGG | - | chr5.3:79265094-79265113 | MS.gene38167:intron | 40.0% | |
| TGTCATGTGGAAGTAATGCA+GGG | + | chr5.3:79264329-79264348 | None:intergenic | 40.0% | |
| !! | CTCAGCTTTAAGCAAAGGTT+TGG | + | chr5.3:79265033-79265052 | None:intergenic | 40.0% |
| !! | TTTGCCTCAGCTTTAAGCAA+AGG | + | chr5.3:79265038-79265057 | None:intergenic | 40.0% |
| AAATCGCGTCACGTTGTAAC+CGG | - | chr5.3:79264424-79264443 | MS.gene38167:CDS | 45.0% | |
| AGCCACAGAAGAGAAAAGTG+AGG | - | chr5.3:79265322-79265341 | MS.gene38167:intron | 45.0% | |
| CGGTGATTCAGTTAATGTCG+AGG | - | chr5.3:79265163-79265182 | MS.gene38167:intron | 45.0% | |
| CTCAACAGGTTCCTTTGTCT+CGG | + | chr5.3:79265129-79265148 | None:intergenic | 45.0% | |
| GCTTAAAGCTGAGGCAAAAG+AGG | - | chr5.3:79265040-79265059 | MS.gene38167:intron | 45.0% | |
| GGAAGAACAAACCGAGACAA+AGG | - | chr5.3:79265115-79265134 | MS.gene38167:intron | 45.0% | |
| GGTGGTAGAAGAAGTTCTTG+TGG | - | chr5.3:79265061-79265080 | MS.gene38167:intron | 45.0% | |
| GGTTGAGAAAGAAGAAGCCT+TGG | - | chr5.3:79265349-79265368 | MS.gene38167:intron | 45.0% | |
| TAAAGCTGAGGCAAAAGAGG+TGG | - | chr5.3:79265043-79265062 | MS.gene38167:intron | 45.0% | |
| TGATAACTGAGCAGTGTCGT+CGG | + | chr5.3:79264916-79264935 | None:intergenic | 45.0% | |
| TGGTAGAGTTACCTCAACAG+TGG | + | chr5.3:79264175-79264194 | None:intergenic | 45.0% | |
| TTCTGTTGTTGAGGCTGTTC+AGG | - | chr5.3:79265205-79265224 | MS.gene38167:intron | 45.0% | |
| ! | TCTTTGGTTTCTGATGCTGC+TGG | + | chr5.3:79264990-79265009 | None:intergenic | 45.0% |
| !! | CTCCTCACTTTTCTCTTCTG+TGG | + | chr5.3:79265327-79265346 | None:intergenic | 45.0% |
| CGAGACAAAGGAACCTGTTG+AGG | - | chr5.3:79265127-79265146 | MS.gene38167:intron | 50.0% | |
| CTCAAACCCTCCCGTTCATA+AGG | - | chr5.3:79264679-79264698 | MS.gene38167:CDS | 50.0% | |
| GGATGAGAATGTGGTAGCTG+AGG | - | chr5.3:79265268-79265287 | MS.gene38167:intron | 50.0% | |
| GGCTGTGAAAGATACAGCAG+AGG | - | chr5.3:79265082-79265101 | MS.gene38167:intron | 50.0% | |
| GGTAGCTGAGGCTGAAACTA+AGG | - | chr5.3:79265280-79265299 | MS.gene38167:intron | 50.0% | |
| TCTGATGCTGCTGGTGTGAT+TGG | + | chr5.3:79264981-79265000 | None:intergenic | 50.0% | |
| TCTGTCTCAGATTCTGCAGC+TGG | + | chr5.3:79265236-79265255 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 79264168 | 79265460 | 79264168 | ID=MS.gene38167 |
| chr5.3 | mRNA | 79264168 | 79265460 | 79264168 | ID=MS.gene38167.t1;Parent=MS.gene38167 |
| chr5.3 | exon | 79265446 | 79265460 | 79265446 | ID=MS.gene38167.t1.exon1;Parent=MS.gene38167.t1 |
| chr5.3 | CDS | 79265446 | 79265460 | 79265446 | ID=cds.MS.gene38167.t1;Parent=MS.gene38167.t1 |
| chr5.3 | exon | 79264168 | 79264740 | 79264168 | ID=MS.gene38167.t1.exon2;Parent=MS.gene38167.t1 |
| chr5.3 | CDS | 79264168 | 79264740 | 79264168 | ID=cds.MS.gene38167.t1;Parent=MS.gene38167.t1 |
| Gene Sequence |
| Protein sequence |
