AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052150


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052150.t1 MTR_1g017080 95.238 189 4 2 1 188 42 226 3.63e-130 370
MS.gene052150.t1 MTR_5g033720 55.882 102 45 0 81 182 72 173 7.37e-34 122
MS.gene052150.t1 MTR_2g024390 51.515 99 48 0 81 179 117 215 1.14e-28 109
MS.gene052150.t1 MTR_5g085860 49.020 102 48 1 82 179 47 148 4.49e-24 96.3
MS.gene052150.t1 MTR_1g084060 46.602 103 49 2 81 182 19 116 3.75e-23 93.2
MS.gene052150.t1 MTR_1g084060 44.118 102 53 1 81 182 19 116 7.36e-23 93.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052150.t1 AT4G08150 54.696 181 63 4 11 187 76 241 2.06e-56 183
MS.gene052150.t1 AT1G23380 51.456 103 50 0 80 182 84 186 1.95e-30 113
MS.gene052150.t1 AT1G62360 50.000 122 59 1 57 176 95 216 1.09e-29 112
MS.gene052150.t1 AT1G23380 50.476 105 50 1 80 182 84 188 1.77e-28 108
MS.gene052150.t1 AT1G70510 48.077 104 54 0 80 183 68 171 2.36e-27 105
MS.gene052150.t1 AT1G70510 46.296 108 54 1 80 183 68 175 3.22e-25 99.8

Find 38 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAGGATGAGCAATGATTT+TGG 0.278077 1.3:+77158171 None:intergenic
ACCATGAATTGGTCTAGTTC+TGG 0.338115 1.3:+77157733 None:intergenic
ACCAAACGAGCCACCACTTC+CGG 0.349272 1.3:+77157823 None:intergenic
ACCGGAAGTGGTGGCTCGTT+TGG 0.365361 1.3:-77157824 MS.gene052150:CDS
AACTTTCATCTTCAATCAAA+TGG 0.370332 1.3:-77158412 MS.gene052150:CDS
TGAAGATGAAAGTTGTTCAT+TGG 0.383854 1.3:+77158421 None:intergenic
AAATCCATAGCCTCTTGTAT+AGG 0.443002 1.3:+77157464 None:intergenic
ATGATGATTATGCATAATAT+TGG 0.443654 1.3:+77158252 None:intergenic
ATATTTGTTCTCATTAAAGC+TGG 0.445786 1.3:+77158286 None:intergenic
TGTTGATGTTGTACTTGATC+AGG 0.447416 1.3:+77158370 None:intergenic
TCCAGAACTAGACCAATTCA+TGG 0.475218 1.3:-77157734 MS.gene052150:intron
CACTTTGCAATGGACCCCTT+CGG 0.484187 1.3:-77157412 MS.gene052150:CDS
GATTGGAGCTCCACCGGAAG+TGG 0.502488 1.3:-77157836 MS.gene052150:CDS
GGACCCCTTCGGATCTTCCC+TGG 0.503797 1.3:-77157401 None:intergenic
AAGGCCTATACAAGAGGCTA+TGG 0.514686 1.3:-77157468 MS.gene052150:CDS
TGGCTCGTTTGGTTGCGTCT+AGG 0.518848 1.3:-77157813 MS.gene052150:CDS
AGCTTACATGGATTGTCAAA+AGG 0.519024 1.3:-77158131 MS.gene052150:intron
CATCATCATCAAGGAGGAGG+AGG 0.530576 1.3:-77158235 MS.gene052150:CDS
GCTTCACCACTAGAACTGCT+TGG 0.545490 1.3:+77158208 None:intergenic
TATGTAGATTGGAGCTCCAC+CGG 0.547316 1.3:-77157842 MS.gene052150:intron
TATTTGTTCTCATTAAAGCT+GGG 0.551107 1.3:+77158287 None:intergenic
ATGCATAATCATCATCATCA+AGG 0.555030 1.3:-77158244 MS.gene052150:CDS
CAGCTAAATACACTTTGCAA+TGG 0.562110 1.3:-77157422 MS.gene052150:CDS
TGGAGCTCCACCGGAAGTGG+TGG 0.570424 1.3:-77157833 MS.gene052150:CDS
TGTAAAAGACTAGAGTATTG+AGG 0.573212 1.3:+77158154 None:intergenic
GGGAGTCCAAGCAGTTCTAG+TGG 0.587384 1.3:-77158214 MS.gene052150:CDS
AATCATCATCATCAAGGAGG+AGG 0.600518 1.3:-77158238 MS.gene052150:CDS
ACTCACCAGGGAAGATCCGA+AGG 0.608503 1.3:+77157396 None:intergenic
ATCATCATCAAGGAGGAGGA+GGG 0.622603 1.3:-77158234 MS.gene052150:CDS
ATTAACAAGGCCTATACAAG+AGG 0.622954 1.3:-77157474 MS.gene052150:CDS
AAACGAGCCACCACTTCCGG+TGG 0.627824 1.3:+77157826 None:intergenic
CATGCTTGTGAAGTATAGAG+AGG 0.648293 1.3:-77157498 MS.gene052150:CDS
CTCACCAGGGAAGATCCGAA+GGG 0.678902 1.3:+77157397 None:intergenic
CTAGGCAAGAGTTTGAAGCA+AGG 0.689197 1.3:-77157795 MS.gene052150:CDS
CATAATCATCATCATCAAGG+AGG 0.690149 1.3:-77158241 MS.gene052150:CDS
TTATGCATAATATTGGTGTG+TGG 0.710252 1.3:+77158259 None:intergenic
AGTATAGAGAGGAATTAACA+AGG 0.714149 1.3:-77157487 MS.gene052150:CDS
TCACCAGGGAAGATCCGAAG+GGG 0.724320 1.3:+77157398 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATATATATATAAAAAAA+TGG + chr1.3:77157923-77157942 None:intergenic 0.0%
!! AAATATATATATAAAAAAAT+GGG + chr1.3:77157922-77157941 None:intergenic 0.0%
!! TTTAAACTTAATAATTATCA+TGG + chr1.3:77158185-77158204 None:intergenic 10.0%
!!! TTTTTAAATTGTTATCATGA+TGG + chr1.3:77157892-77157911 None:intergenic 15.0%
!! ATGATGATTATGCATAATAT+TGG + chr1.3:77157571-77157590 None:intergenic 20.0%
!! TAAACAAACTATATGTAGAT+TGG - chr1.3:77157967-77157986 MS.gene052150:intron 20.0%
!! TTTCAGTCAGAAATTAAATT+TGG - chr1.3:77157859-77157878 MS.gene052150:intron 20.0%
!!! ATTAGAACTTATAGATTTTG+TGG - chr1.3:77158251-77158270 MS.gene052150:CDS 20.0%
! AAATGGTGTTTATGTTTAAC+AGG - chr1.3:77158286-77158305 MS.gene052150:CDS 25.0%
! AACTTTCATCTTCAATCAAA+TGG - chr1.3:77157408-77157427 MS.gene052150:CDS 25.0%
! ATATTTGTTCTCATTAAAGC+TGG + chr1.3:77157537-77157556 None:intergenic 25.0%
! TAAATTGTTATCATGATGGT+GGG + chr1.3:77157888-77157907 None:intergenic 25.0%
! TATTTGTTCTCATTAAAGCT+GGG + chr1.3:77157536-77157555 None:intergenic 25.0%
! TTAAATTGTTATCATGATGG+TGG + chr1.3:77157889-77157908 None:intergenic 25.0%
! TTAATCTCAAATTTAGTGGA+AGG - chr1.3:77157802-77157821 MS.gene052150:CDS 25.0%
! TTGGTTAATCTCAAATTTAG+TGG - chr1.3:77157798-77157817 MS.gene052150:CDS 25.0%
AAAGCTGGGTAATGAAAAAT+CGG + chr1.3:77157522-77157541 None:intergenic 30.0%
AGTATAGAGAGGAATTAACA+AGG - chr1.3:77158333-77158352 MS.gene052150:CDS 30.0%
ATGCATAATCATCATCATCA+AGG - chr1.3:77157576-77157595 MS.gene052150:intron 30.0%
TTATGCATAATATTGGTGTG+TGG + chr1.3:77157564-77157583 None:intergenic 30.0%
! TAGTCTTTTACAAGCTTACA+TGG - chr1.3:77157677-77157696 MS.gene052150:intron 30.0%
!! TGTAAAAGACTAGAGTATTG+AGG + chr1.3:77157669-77157688 None:intergenic 30.0%
!!! AGGGGGTATATTTTGTTTTA+TGG - chr1.3:77158113-77158132 MS.gene052150:intron 30.0%
AAATCCATAGCCTCTTGTAT+AGG + chr1.3:77158359-77158378 None:intergenic 35.0%
ATTAACAAGGCCTATACAAG+AGG - chr1.3:77158346-77158365 MS.gene052150:CDS 35.0%
CAGCTAAATACACTTTGCAA+TGG - chr1.3:77158398-77158417 MS.gene052150:CDS 35.0%
CATAATCATCATCATCAAGG+AGG - chr1.3:77157579-77157598 MS.gene052150:intron 35.0%
TAGACCAATTCATGGTACAA+GGG - chr1.3:77158094-77158113 MS.gene052150:intron 35.0%
TGTTGATGTTGTACTTGATC+AGG + chr1.3:77157453-77157472 None:intergenic 35.0%
TTCAAGAGAAAAAGAGGGTT+TGG - chr1.3:77157779-77157798 MS.gene052150:CDS 35.0%
! AAACCCTCTTTTTCTCTTGA+AGG + chr1.3:77157780-77157799 None:intergenic 35.0%
! AACCCTCTTTTTCTCTTGAA+GGG + chr1.3:77157779-77157798 None:intergenic 35.0%
! AGCTTACATGGATTGTCAAA+AGG - chr1.3:77157689-77157708 MS.gene052150:intron 35.0%
! TTGAGGATGAGCAATGATTT+TGG + chr1.3:77157652-77157671 None:intergenic 35.0%
!!! TGTGGTTTTAGACGTTGAAA+TGG - chr1.3:77158269-77158288 MS.gene052150:CDS 35.0%
AATCATCATCATCAAGGAGG+AGG - chr1.3:77157582-77157601 MS.gene052150:intron 40.0%
AGACCAATTCATGGTACAAG+GGG - chr1.3:77158095-77158114 MS.gene052150:intron 40.0%
CATGCTTGTGAAGTATAGAG+AGG - chr1.3:77158322-77158341 MS.gene052150:CDS 40.0%
CTAGACCAATTCATGGTACA+AGG - chr1.3:77158093-77158112 MS.gene052150:intron 40.0%
TCCAGAACTAGACCAATTCA+TGG - chr1.3:77158086-77158105 MS.gene052150:intron 40.0%
TGCCCTTCAAGAGAAAAAGA+GGG - chr1.3:77157774-77157793 MS.gene052150:CDS 40.0%
! ACCATGAATTGGTCTAGTTC+TGG + chr1.3:77158090-77158109 None:intergenic 40.0%
! TCTTTTTCTCTTGAAGGGCA+GGG + chr1.3:77157774-77157793 None:intergenic 40.0%
! TTCGATTTGCACTGTGATGT+CGG - chr1.3:77158210-77158229 MS.gene052150:CDS 40.0%
AAGGCCTATACAAGAGGCTA+TGG - chr1.3:77158352-77158371 MS.gene052150:CDS 45.0%
ATCATCATCAAGGAGGAGGA+GGG - chr1.3:77157586-77157605 MS.gene052150:intron 45.0%
CTGCCCTTCAAGAGAAAAAG+AGG - chr1.3:77157773-77157792 MS.gene052150:CDS 45.0%
GACCAATTCATGGTACAAGG+GGG - chr1.3:77158096-77158115 MS.gene052150:intron 45.0%
TACCCCCTTGTACCATGAAT+TGG + chr1.3:77158101-77158120 None:intergenic 45.0%
TATGTAGATTGGAGCTCCAC+CGG - chr1.3:77157978-77157997 MS.gene052150:intron 45.0%
! CTAGGCAAGAGTTTGAAGCA+AGG - chr1.3:77158025-77158044 MS.gene052150:intron 45.0%
! CTCTTTTTCTCTTGAAGGGC+AGG + chr1.3:77157775-77157794 None:intergenic 45.0%
!! AATATATATATAAAAAAATG+GGG + chr1.3:77157921-77157940 None:intergenic 5.0%
CACTTTGCAATGGACCCCTT+CGG - chr1.3:77158408-77158427 MS.gene052150:CDS 50.0%
CATCATCATCAAGGAGGAGG+AGG - chr1.3:77157585-77157604 MS.gene052150:intron 50.0%
GCTTCACCACTAGAACTGCT+TGG + chr1.3:77157615-77157634 None:intergenic 50.0%
ACCAAACGAGCCACCACTTC+CGG + chr1.3:77158000-77158019 None:intergenic 55.0%
TGGCTCGTTTGGTTGCGTCT+AGG - chr1.3:77158007-77158026 MS.gene052150:intron 55.0%
! GGGAGTCCAAGCAGTTCTAG+TGG - chr1.3:77157606-77157625 MS.gene052150:intron 55.0%
AAACGAGCCACCACTTCCGG+TGG + chr1.3:77157997-77158016 None:intergenic 60.0%
ACCGGAAGTGGTGGCTCGTT+TGG - chr1.3:77157996-77158015 MS.gene052150:intron 60.0%
GATTGGAGCTCCACCGGAAG+TGG - chr1.3:77157984-77158003 MS.gene052150:intron 60.0%
TGGAGCTCCACCGGAAGTGG+TGG - chr1.3:77157987-77158006 MS.gene052150:intron 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 77157402 77158440 77157402 ID=MS.gene052150
chr1.3 mRNA 77157402 77158440 77157402 ID=MS.gene052150.t1;Parent=MS.gene052150
chr1.3 exon 77158132 77158440 77158132 ID=MS.gene052150.t1.exon1;Parent=MS.gene052150.t1
chr1.3 CDS 77158132 77158440 77158132 ID=cds.MS.gene052150.t1;Parent=MS.gene052150.t1
chr1.3 exon 77157735 77157857 77157735 ID=MS.gene052150.t1.exon2;Parent=MS.gene052150.t1
chr1.3 CDS 77157735 77157857 77157735 ID=cds.MS.gene052150.t1;Parent=MS.gene052150.t1
chr1.3 exon 77157402 77157534 77157402 ID=MS.gene052150.t1.exon3;Parent=MS.gene052150.t1
chr1.3 CDS 77157402 77157534 77157402 ID=cds.MS.gene052150.t1;Parent=MS.gene052150.t1
Gene Sequence

>MS.gene052150

ATGAACAACTTTCATCTTCAATCAAATGGATCAGACAATTGTTTTCAACCTGATCAAGTACAACATCAACACTCTGCTGTGAAAACTGAAGCAAATAGTACTTCACAACTTCACACTCCGATTTTTCATTACCCAGCTTTAATGAGAACAAATATTATTCCACACACCAATATTATGCATAATCATCATCATCAAGGAGGAGGAGGGAGTCCAAGCAGTTCTAGTGGTGAAGCTGAAGCTATAAAAGCCAAAATCATTGCTCATCCTCAATACTCTAGTCTTTTACAAGCTTACATGGATTGTCAAAAGGTCACTAGTTAATTATTATTTTTATTCATTTTCATCATTATAACAATTTATTTTTCTTAACCCTGCCCTTCAAGAGAAAAAGAGGGTTTGGTTAATCTCAAATTTAGTGGAAGGTAAGTAAAATAGTATAATCAATAATTAATGATTGTTTCAGTCAGAAATTAAATTTGGACTCCCACCATCATGATAACAATTTAAAAAAGCATACCCCATTTTTTTATATATATATTTTAATTAATTGCTATATCTCTTTAATTAAACAAACTATATGTAGATTGGAGCTCCACCGGAAGTGGTGGCTCGTTTGGTTGCGTCTAGGCAAGAGTTTGAAGCAAGGCAAAGATCTTCAGTTAACTCAAGAGAAGCTTCTAAAGATCCAGAACTAGACCAATTCATGGTACAAGGGGGTATATTTTGTTTTATGGTTTATGTTTTTTTAAAAAAATAAAAATAATTTTCGTTTAGTTTTTACCATGATAATTATTAAGTTTAAATTATGTTCGATTTGCACTGTGATGTCGGCAAAAATCTTTATTATTTATTAGAACTTATAGATTTTGTGGTTTTAGACGTTGAAATGGTGTTTATGTTTAACAGGAAGCATATTATGACATGCTTGTGAAGTATAGAGAGGAATTAACAAGGCCTATACAAGAGGCTATGGATTTCATGAGAAGAATAGAAACTCAGCTAAATACACTTTGCAATGGACCCCTTCGGATCTTCCCTG

Protein sequence

>MS.gene052150.t1

MNNFHLQSNGSDNCFQPDQVQHQHSAVKTEANSTSQLHTPIFHYPALMRTNIIPHTNIMHNHHHQGGGGSPSSSSGEAEAIKAKIIAHPQYSSLLQAYMDCQKIGAPPEVVARLVASRQEFEARQRSSVNSREASKDPELDQFMEAYYDMLVKYREELTRPIQEAMDFMRRIETQLNTLCNGPLRIFP