Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38349.t1 | XP_003603039.1 | 100 | 86 | 0 | 0 | 1 | 86 | 1 | 86 | 5.50E-28 | 133.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38349.t1 | G7J6D3 | 100.0 | 86 | 0 | 0 | 1 | 86 | 1 | 86 | 4.0e-28 | 133.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049596 | MS.gene38349 | 0.815073 | 1.11E-51 | -1.69E-46 |
| MS.gene050297 | MS.gene38349 | 0.853157 | 2.88E-61 | -1.69E-46 |
| MS.gene054643 | MS.gene38349 | 0.869618 | 2.71E-66 | -1.69E-46 |
| MS.gene058844 | MS.gene38349 | 0.826549 | 2.55E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38349.t1 | MTR_3g101680 | 100.000 | 86 | 0 | 0 | 1 | 86 | 1 | 86 | 9.71e-54 | 176 |
| MS.gene38349.t1 | MTR_4g086240 | 43.000 | 100 | 38 | 4 | 1 | 86 | 1 | 95 | 5.37e-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGCTTAAGAGCTTTGAGTT+TGG | 0.397722 | 3.2:+84662774 | None:intergenic |
| AGATAAAGCTTAACGGCGTT+AGG | 0.399449 | 3.2:+84662727 | None:intergenic |
| GATAAAGCTTAACGGCGTTA+GGG | 0.483757 | 3.2:+84662728 | None:intergenic |
| GATGTGAAGATAAAGCTTAA+CGG | 0.516648 | 3.2:+84662720 | None:intergenic |
| GTGGCGCTCACAGCCGCGAC+GGG | 0.543026 | 3.2:-84662924 | MS.gene38349:CDS |
| GGTGGCGCTCACAGCCGCGA+CGG | 0.558055 | 3.2:-84662925 | MS.gene38349:CDS |
| AAGCTTTATCTTCACATCGG+TGG | 0.566382 | 3.2:-84662715 | MS.gene38349:CDS |
| GTTAAGCTTTATCTTCACAT+CGG | 0.621435 | 3.2:-84662718 | MS.gene38349:CDS |
| TCAACTTCATCTACAGATGA+CGG | 0.624942 | 3.2:+84662799 | None:intergenic |
| AACTTTCGAGTAACCCGTCG+CGG | 0.640215 | 3.2:+84662911 | None:intergenic |
| AACTACGAAGATGCAAACGA+AGG | 0.673113 | 3.2:-84662850 | MS.gene38349:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTTCTTCGTATTCTTCAT+CGG | + | chr3.2:84662736-84662755 | None:intergenic | 25.0% |
| GATGTGAAGATAAAGCTTAA+CGG | + | chr3.2:84662904-84662923 | None:intergenic | 30.0% | |
| ! | GTTAAGCTTTATCTTCACAT+CGG | - | chr3.2:84662903-84662922 | MS.gene38349:CDS | 30.0% |
| TCAACTTCATCTACAGATGA+CGG | + | chr3.2:84662825-84662844 | None:intergenic | 35.0% | |
| !! | AAGCTTAAGAGCTTTGAGTT+TGG | + | chr3.2:84662850-84662869 | None:intergenic | 35.0% |
| AACTACGAAGATGCAAACGA+AGG | - | chr3.2:84662771-84662790 | MS.gene38349:CDS | 40.0% | |
| AGATAAAGCTTAACGGCGTT+AGG | + | chr3.2:84662897-84662916 | None:intergenic | 40.0% | |
| GATAAAGCTTAACGGCGTTA+GGG | + | chr3.2:84662896-84662915 | None:intergenic | 40.0% | |
| ! | AAGCTTTATCTTCACATCGG+TGG | - | chr3.2:84662906-84662925 | MS.gene38349:CDS | 40.0% |
| GTAATTCCCCCAACGCAAAA+TGG | - | chr3.2:84662928-84662947 | MS.gene38349:CDS | 45.0% | |
| ! | AACTTTCGAGTAACCCGTCG+CGG | + | chr3.2:84662713-84662732 | None:intergenic | 50.0% |
| GGTGGCGCTCACAGCCGCGA+CGG | - | chr3.2:84662696-84662715 | MS.gene38349:CDS | 75.0% | |
| GTGGCGCTCACAGCCGCGAC+GGG | - | chr3.2:84662697-84662716 | MS.gene38349:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 84662693 | 84662950 | 84662693 | ID=MS.gene38349 |
| chr3.2 | mRNA | 84662693 | 84662950 | 84662693 | ID=MS.gene38349.t1;Parent=MS.gene38349 |
| chr3.2 | exon | 84662693 | 84662950 | 84662693 | ID=MS.gene38349.t1.exon1;Parent=MS.gene38349.t1 |
| chr3.2 | CDS | 84662693 | 84662950 | 84662693 | ID=cds.MS.gene38349.t1;Parent=MS.gene38349.t1 |
| Gene Sequence |
| Protein sequence |
