AlfalfaGEDB | MS.gene058844

M. sativa cultivar XinJiangDaYe / MS.gene058844

KEGG: K11251
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF00125.27 PF16211.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058844.t1	RHN60978.1	96.4	138	4	1	1	137	1	138	2.90E-64	254.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058844.t1	Q1S053	96.2	133	5	0	1	133	1	133	9.9e-66	250.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058844.t1	A0A396I838	96.4	138	4	1	1	137	1	138	2.1e-64	254.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049596	MS.gene058844	0.899973	1.17E-77	-1.69E-46
MS.gene04972	MS.gene058844	0.825772	3.91E-54	-1.69E-46
MS.gene049989	MS.gene058844	0.841892	3.61E-58	-1.69E-46
MS.gene050297	MS.gene058844	0.871309	7.54E-67	-1.69E-46
MS.gene050648	MS.gene058844	0.8168	4.59E-52	-1.69E-46
MS.gene05124	MS.gene058844	0.884206	2.34E-71	-1.69E-46
MS.gene051263	MS.gene058844	0.80469	1.91E-49	-1.69E-46
MS.gene052143	MS.gene058844	0.814923	1.20E-51	-1.69E-46
MS.gene052367	MS.gene058844	0.813651	2.30E-51	-1.69E-46
MS.gene053173	MS.gene058844	0.82889	6.99E-55	-1.69E-46
MS.gene053416	MS.gene058844	0.836695	8.05E-57	-1.69E-46
MS.gene053417	MS.gene058844	0.836489	9.08E-57	-1.69E-46
MS.gene054551	MS.gene058844	0.807864	4.10E-50	-1.69E-46
MS.gene054643	MS.gene058844	0.887613	1.22E-72	-1.69E-46
MS.gene055957	MS.gene058844	0.829035	6.45E-55	-1.69E-46
MS.gene057458	MS.gene058844	0.80751	4.88E-50	-1.69E-46
MS.gene057483	MS.gene058844	0.819643	1.04E-52	-1.69E-46
MS.gene05820	MS.gene058844	0.827139	1.84E-54	-1.69E-46
MS.gene058818	MS.gene058844	0.882093	1.39E-70	-1.69E-46
MS.gene058844	MS.gene06057	0.818467	1.93E-52	-1.69E-46
MS.gene058844	MS.gene06093	0.835197	1.93E-56	-1.69E-46
MS.gene058844	MS.gene061260	0.828654	7.97E-55	-1.69E-46
MS.gene058844	MS.gene061275	0.809839	1.55E-50	-1.69E-46
MS.gene058844	MS.gene061303	0.800237	1.58E-48	-1.69E-46
MS.gene058844	MS.gene061805	0.825167	5.43E-54	-1.69E-46
MS.gene058844	MS.gene064834	0.810456	1.14E-50	-1.69E-46
MS.gene058844	MS.gene066026	0.818449	1.95E-52	-1.69E-46
MS.gene058844	MS.gene066031	0.85494	8.80E-62	-1.69E-46
MS.gene058844	MS.gene066551	0.806196	9.25E-50	-1.69E-46
MS.gene058844	MS.gene066552	0.816225	6.17E-52	-1.69E-46
MS.gene058844	MS.gene066875	0.803717	3.05E-49	-1.69E-46
MS.gene058844	MS.gene067030	0.854118	1.52E-61	-1.69E-46
MS.gene058844	MS.gene067039	0.843763	1.15E-58	-1.69E-46
MS.gene058844	MS.gene067143	0.805174	1.52E-49	-1.69E-46
MS.gene058844	MS.gene068305	0.817546	3.12E-52	-1.69E-46
MS.gene058844	MS.gene068547	0.819141	1.36E-52	-1.69E-46
MS.gene058844	MS.gene068548	0.804373	2.23E-49	-1.69E-46
MS.gene058844	MS.gene069090	0.806861	6.70E-50	-1.69E-46
MS.gene058844	MS.gene069140	0.800105	1.68E-48	-1.69E-46
MS.gene058844	MS.gene07028	0.806324	8.69E-50	-1.69E-46
MS.gene058844	MS.gene07048	0.815682	8.16E-52	-1.69E-46
MS.gene058844	MS.gene07051	0.807421	5.10E-50	-1.69E-46
MS.gene058844	MS.gene071185	0.806684	7.30E-50	-1.69E-46
MS.gene058844	MS.gene071851	0.832099	1.15E-55	-1.69E-46
MS.gene058844	MS.gene073862	0.834575	2.77E-56	-1.69E-46
MS.gene058844	MS.gene073863	0.825608	4.27E-54	-1.69E-46
MS.gene058844	MS.gene07425	0.831879	1.30E-55	-1.69E-46
MS.gene058844	MS.gene08109	0.840005	1.13E-57	-1.69E-46
MS.gene058844	MS.gene08860	0.816269	6.03E-52	-1.69E-46
MS.gene058844	MS.gene09234	0.832953	7.04E-56	-1.69E-46
MS.gene058844	MS.gene20224	0.845879	3.08E-59	-1.69E-46
MS.gene058844	MS.gene21320	0.843479	1.37E-58	-1.69E-46
MS.gene058844	MS.gene22500	0.801054	1.08E-48	-1.69E-46
MS.gene058844	MS.gene23323	0.831822	1.34E-55	-1.69E-46
MS.gene058844	MS.gene23886	0.805999	1.02E-49	-1.69E-46
MS.gene058844	MS.gene24699	0.866232	3.31E-65	-1.69E-46
MS.gene058844	MS.gene26224	0.817787	2.75E-52	-1.69E-46
MS.gene058844	MS.gene27823	0.869539	2.87E-66	-1.69E-46
MS.gene058844	MS.gene27941	0.804111	2.53E-49	-1.69E-46
MS.gene058844	MS.gene28050	0.818533	1.86E-52	-1.69E-46
MS.gene058844	MS.gene28787	0.809843	1.55E-50	-1.69E-46
MS.gene058844	MS.gene28867	0.808934	2.43E-50	-1.69E-46
MS.gene058844	MS.gene30854	0.806029	1.00E-49	-1.69E-46
MS.gene058844	MS.gene32306	0.822334	2.50E-53	-1.69E-46
MS.gene058844	MS.gene32703	0.8313	1.81E-55	-1.69E-46
MS.gene058844	MS.gene32740	0.808028	3.79E-50	-1.69E-46
MS.gene058844	MS.gene35715	0.806065	9.86E-50	-1.69E-46
MS.gene058844	MS.gene36485	0.823746	1.17E-53	-1.69E-46
MS.gene058844	MS.gene37495	0.800315	1.53E-48	-1.69E-46
MS.gene058844	MS.gene37573	0.835658	1.48E-56	-1.69E-46
MS.gene058844	MS.gene38349	0.826549	2.55E-54	-1.69E-46
MS.gene058844	MS.gene38714	0.885567	7.27E-72	-1.69E-46
MS.gene058844	MS.gene38828	0.810315	1.23E-50	-1.69E-46
MS.gene058844	MS.gene38942	0.837914	3.92E-57	-1.69E-46
MS.gene058844	MS.gene39058	0.814745	1.32E-51	-1.69E-46
MS.gene058844	MS.gene40329	0.80321	3.89E-49	-1.69E-46
MS.gene058844	MS.gene41161	0.820377	7.08E-53	-1.69E-46
MS.gene058844	MS.gene42665	0.835613	1.52E-56	-1.69E-46
MS.gene058844	MS.gene44222	0.827482	1.53E-54	-1.69E-46
MS.gene058844	MS.gene44251	0.817295	3.55E-52	-1.69E-46
MS.gene058844	MS.gene47007	0.849797	2.57E-60	-1.69E-46
MS.gene058844	MS.gene47280	0.832059	1.17E-55	-1.69E-46
MS.gene058844	MS.gene50734	0.83678	7.66E-57	-1.69E-46
MS.gene058844	MS.gene50736	0.809137	2.20E-50	-1.69E-46
MS.gene058844	MS.gene52607	0.809691	1.67E-50	-1.69E-46
MS.gene058844	MS.gene52703	0.824091	9.74E-54	-1.69E-46
MS.gene058844	MS.gene52975	0.86231	5.55E-64	-1.69E-46
MS.gene058844	MS.gene52980	0.822993	1.76E-53	-1.69E-46
MS.gene058844	MS.gene53887	0.864008	1.66E-64	-1.69E-46
MS.gene058844	MS.gene55687	0.812326	4.49E-51	-1.69E-46
MS.gene058844	MS.gene55782	0.810718	1.00E-50	-1.69E-46
MS.gene058844	MS.gene55817	0.836642	8.30E-57	-1.69E-46
MS.gene058844	MS.gene57219	0.80431	2.30E-49	-1.69E-46
MS.gene058844	MS.gene57320	0.82513	5.54E-54	-1.69E-46
MS.gene058844	MS.gene57750	0.826747	2.29E-54	-1.69E-46
MS.gene058844	MS.gene57752	0.80607	9.83E-50	-1.69E-46
MS.gene058844	MS.gene58096	0.816578	5.14E-52	-1.69E-46
MS.gene058844	MS.gene58754	0.800441	1.44E-48	-1.69E-46
MS.gene058844	MS.gene58835	0.813551	2.42E-51	-1.69E-46
MS.gene058844	MS.gene59357	0.801667	8.08E-49	-1.69E-46
MS.gene058844	MS.gene59466	0.82101	5.07E-53	-1.69E-46
MS.gene058844	MS.gene60598	0.802972	4.35E-49	-1.69E-46
MS.gene058844	MS.gene61977	0.817585	3.06E-52	-1.69E-46
MS.gene058844	MS.gene64852	0.812841	3.46E-51	-1.69E-46
MS.gene058844	MS.gene64859	0.81284	3.46E-51	-1.69E-46
MS.gene058844	MS.gene65301	0.808198	3.48E-50	-1.69E-46
MS.gene058844	MS.gene66158	0.820748	5.82E-53	-1.69E-46
MS.gene058844	MS.gene66159	0.832829	7.56E-56	-1.69E-46
MS.gene058844	MS.gene66429	0.808685	2.74E-50	-1.69E-46
MS.gene058844	MS.gene66433	0.808619	2.83E-50	-1.69E-46
MS.gene058844	MS.gene67555	0.800478	1.41E-48	-1.69E-46
MS.gene058844	MS.gene67576	0.84111	5.80E-58	-1.69E-46
MS.gene058844	MS.gene68101	0.806148	9.47E-50	-1.69E-46
MS.gene058844	MS.gene69328	0.809589	1.76E-50	-1.69E-46
MS.gene058844	MS.gene69952	0.816864	4.44E-52	-1.69E-46
MS.gene058844	MS.gene70240	0.810314	1.23E-50	-1.69E-46
MS.gene058844	MS.gene70261	0.816356	5.77E-52	-1.69E-46
MS.gene058844	MS.gene70263	0.801208	1.00E-48	-1.69E-46
MS.gene058844	MS.gene70838	0.806076	9.80E-50	-1.69E-46
MS.gene058844	MS.gene70896	0.833595	4.87E-56	-1.69E-46
MS.gene058844	MS.gene70898	0.832664	8.31E-56	-1.69E-46
MS.gene058844	MS.gene70990	0.811011	8.66E-51	-1.69E-46
MS.gene058844	MS.gene71419	0.843972	1.01E-58	-1.69E-46
MS.gene058844	MS.gene71431	0.824669	7.12E-54	-1.69E-46
MS.gene058844	MS.gene72088	0.83312	6.40E-56	-1.69E-46
MS.gene058844	MS.gene72127	0.80353	3.34E-49	-1.69E-46
MS.gene058844	MS.gene72129	0.800344	1.51E-48	-1.69E-46
MS.gene058844	MS.gene74328	0.819198	1.32E-52	-1.69E-46
MS.gene058844	MS.gene75320	0.827575	1.45E-54	-1.69E-46
MS.gene058844	MS.gene75567	0.828421	9.08E-55	-1.69E-46
MS.gene058844	MS.gene75579	0.847987	8.17E-60	-1.69E-46
MS.gene058844	MS.gene75642	0.829451	5.11E-55	-1.69E-46
MS.gene058844	MS.gene75737	0.8117	6.14E-51	-1.69E-46
MS.gene058844	MS.gene77475	0.830061	3.63E-55	-1.69E-46
MS.gene058844	MS.gene78112	0.812098	5.03E-51	-1.69E-46
MS.gene058844	MS.gene78156	0.814485	1.50E-51	-1.69E-46
MS.gene058844	MS.gene78972	0.854914	8.96E-62	-1.69E-46
MS.gene058844	MS.gene79126	0.820323	7.29E-53	-1.69E-46
MS.gene058844	MS.gene79921	0.822717	2.04E-53	-1.69E-46
MS.gene058844	MS.gene80180	0.840399	8.90E-58	-1.69E-46
MS.gene058844	MS.gene80183	0.835838	1.33E-56	-1.69E-46
MS.gene058844	MS.gene84014	0.8016	8.34E-49	-1.69E-46
MS.gene058844	MS.gene84088	0.826262	2.99E-54	-1.69E-46
MS.gene058844	MS.gene85599	0.808549	2.93E-50	-1.69E-46
MS.gene058844	MS.gene89565	0.808309	3.30E-50	-1.69E-46
MS.gene058844	MS.gene90876	0.840463	8.56E-58	-1.69E-46
MS.gene058844	MS.gene93102	0.835989	1.22E-56	-1.69E-46
MS.gene058844	MS.gene97703	0.814065	1.86E-51	-1.69E-46
MS.gene058844	MS.gene99093	0.82442	8.15E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058844.t1	MTR_8g086640	98.000	150	3	0	1	150	1	150	3.92e-101	286
MS.gene058844.t1	MTR_4g063280	95.455	132	5	1	20	150	14	145	1.59e-84	244
MS.gene058844.t1	MTR_2g096610	87.333	150	17	1	1	150	1	148	1.63e-83	242
MS.gene058844.t1	MTR_4g064967	94.000	150	7	1	1	150	1	148	2.88e-81	236
MS.gene058844.t1	MTR_4g071150	91.503	153	9	2	1	150	1	152	8.92e-80	233
MS.gene058844.t1	MTR_4g064005	91.333	150	11	1	1	150	1	148	4.09e-79	231
MS.gene058844.t1	MTR_4g063410	90.667	150	12	1	1	150	1	148	9.41e-79	230
MS.gene058844.t1	MTR_2g096570	89.610	154	11	3	1	150	1	153	6.78e-78	228
MS.gene058844.t1	MTR_7g113160	81.955	133	23	1	10	142	13	144	2.68e-72	213
MS.gene058844.t1	MTR_7g108320	70.213	141	39	2	3	142	2	140	1.69e-60	183
MS.gene058844.t1	MTR_7g114060	70.000	140	38	1	3	142	2	137	7.19e-60	182
MS.gene058844.t1	MTR_7g114040	70.000	140	38	1	3	142	2	137	7.19e-60	182
MS.gene058844.t1	MTR_2g082510	74.790	119	30	0	11	129	3	121	1.62e-58	178
MS.gene058844.t1	MTR_7g093910	72.222	126	32	1	4	129	5	127	2.60e-58	178
MS.gene058844.t1	MTR_2g082220	73.950	119	31	0	11	129	3	121	2.40e-57	175
MS.gene058844.t1	MTR_6g005150	64.567	127	31	3	24	149	26	139	6.55e-48	151
MS.gene058844.t1	MTR_7g114050	65.385	130	30	3	17	142	10	128	2.08e-46	147
MS.gene058844.t1	MTR_4g063300	76.190	105	20	1	45	149	10	109	7.34e-46	145
MS.gene058844.t1	MTR_1g011750	59.292	113	44	2	21	132	23	134	5.99e-36	121
MS.gene058844.t1	MTR_1g011850	59.292	113	44	2	21	132	23	134	5.99e-36	121
MS.gene058844.t1	MTR_1g114050	59.633	109	42	2	21	128	20	127	5.83e-35	118
MS.gene058844.t1	MTR_1g071040	61.538	104	38	2	26	128	28	130	2.86e-34	117
MS.gene058844.t1	MTR_1g069770	53.333	90	41	1	45	133	9	98	1.46e-24	91.3
MS.gene058844.t1	MTR_7g010440	70.588	51	15	0	78	128	122	172	3.09e-16	73.9
MS.gene058844.t1	MTR_4g048490	43.810	105	30	2	24	128	26	101	3.42e-16	70.1
MS.gene058844.t1	MTR_4g005910	74.419	43	11	0	87	129	4	46	5.10e-16	68.2
MS.gene058844.t1	MTR_7g027610	40.517	116	43	3	20	134	10	100	3.12e-15	67.8
MS.gene058844.t1	MTR_2g096590	72.500	40	11	0	110	149	12	51	3.03e-12	58.2
MS.gene058844.t1	MTR_0653s0030	53.704	54	25	0	56	109	44	97	1.38e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058844.t1	AT5G02560	82.353	153	24	1	1	150	1	153	2.22e-80	234
MS.gene058844.t1	AT5G02560	71.186	177	24	2	1	150	1	177	2.21e-75	223
MS.gene058844.t1	AT5G27670	75.000	152	34	3	1	150	1	150	1.39e-72	214
MS.gene058844.t1	AT5G59870	75.000	148	36	1	4	150	3	150	8.98e-72	212
MS.gene058844.t1	AT1G51060	76.667	120	28	0	11	130	3	122	9.15e-61	184
MS.gene058844.t1	AT4G27230	75.630	119	29	0	11	129	3	121	1.76e-59	181
MS.gene058844.t1	AT4G27230	75.630	119	29	0	11	129	3	121	1.76e-59	181
MS.gene058844.t1	AT3G20670	75.630	119	29	0	11	129	3	121	7.39e-59	179
MS.gene058844.t1	AT1G08880	71.318	129	34	1	7	132	2	130	1.84e-58	178
MS.gene058844.t1	AT5G54640	74.790	119	30	0	11	129	3	121	4.41e-58	177
MS.gene058844.t1	AT1G54690	72.800	125	31	1	11	132	6	130	8.23e-58	177
MS.gene058844.t1	AT2G38810	59.821	112	43	2	21	131	25	135	1.96e-36	122
MS.gene058844.t1	AT2G38810	59.821	112	43	2	21	131	25	135	1.96e-36	122
MS.gene058844.t1	AT2G38810	59.821	112	43	2	21	131	25	135	1.96e-36	122
MS.gene058844.t1	AT2G38810	59.821	112	43	2	21	131	25	135	1.96e-36	122
MS.gene058844.t1	AT1G52740	59.633	109	42	2	25	132	27	134	1.08e-34	118
MS.gene058844.t1	AT3G54560	58.716	109	43	2	25	132	29	136	1.73e-34	117
MS.gene058844.t1	AT3G54560	58.716	109	43	2	25	132	29	136	1.73e-34	117
MS.gene058844.t1	AT4G13570	47.826	92	46	2	32	122	28	118	8.57e-20	79.7
MS.gene058844.t1	AT4G13570	47.253	91	46	2	33	122	35	124	2.67e-19	78.6

Find 45 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTAGCCTTAGAAGGAGATTT+AGG	0.266583	4.3:+13848379	None:intergenic
AAACAAATCTAAGCCTTCTT+AGG	0.296900	4.3:+13848349	None:intergenic
GGAGATTTCTTAGCCTTAGA+AGG	0.321412	4.3:+13848370	None:intergenic
CAAAAGCACAGGGTTAATAT+TGG	0.326164	4.3:+13848432	None:intergenic
CAATTCCCAGTCGGAAGAAT+TGG	0.342053	4.3:-13848688	MS.gene058844:CDS
CTTGTTACCGACTTCTTCCT+TGG	0.365854	4.3:+13848730	None:intergenic
TGCAGCTGAAGTTCTTGAAT+TGG	0.368829	4.3:-13848584	MS.gene058844:CDS
GGAGCCTAAATCTCCTTCTA+AGG	0.376066	4.3:-13848383	MS.gene058844:CDS
GAAAACTGAGAAAGCTGTTA+AGG	0.380382	4.3:-13848404	MS.gene058844:CDS
GAGCTTGGGAAATTGCTTAC+TGG	0.397564	4.3:-13848484	MS.gene058844:CDS
AGCACAAAGGCGACGAAGAA+AGG	0.403145	4.3:-13848778	MS.gene058844:CDS
AAAAGCACAGGGTTAATATT+GGG	0.429889	4.3:+13848433	None:intergenic
AGAACAGCGGCTAAGTAAAC+AGG	0.447906	4.3:+13848616	None:intergenic
GCTGAAGTTCTTGAATTGGC+TGG	0.464803	4.3:-13848580	MS.gene058844:CDS
TCCGACTGGGAATTGAAGTC+CGG	0.472609	4.3:+13848696	None:intergenic
GAAGGAAAGGCGGAGGACCA+AGG	0.493860	4.3:-13848747	MS.gene058844:CDS
TATACCAAGGCATGTGTTGT+TGG	0.495755	4.3:-13848524	MS.gene058844:CDS
TACGCTCAACGTGTCGGCAC+CGG	0.496373	4.3:-13848643	MS.gene058844:CDS
GGCTAAGAAATCTCCTAAGA+AGG	0.510257	4.3:-13848362	MS.gene058844:CDS
ATTGGCCGTTACTTGAAGAA+AGG	0.513109	4.3:-13848670	MS.gene058844:CDS
TAACGGCCAATTCTTCCGAC+TGG	0.515556	4.3:+13848682	None:intergenic
AAAGGAGCTGGAGGAAGGAA+AGG	0.517528	4.3:-13848760	MS.gene058844:CDS
CACGTGATAACAAGAAGAAT+CGG	0.520472	4.3:-13848549	MS.gene058844:CDS
CGAAGAAAGGAGCTGGAGGA+AGG	0.526335	4.3:-13848765	MS.gene058844:CDS
GCTGGAGGAAGGAAAGGCGG+AGG	0.531971	4.3:-13848754	MS.gene058844:CDS
AAGGCGACGAAGAAAGGAGC+TGG	0.543027	4.3:-13848772	MS.gene058844:CDS
GTGAGGAATGATGAAGAGCT+TGG	0.543487	4.3:-13848499	MS.gene058844:CDS
ACTGGTGTTACCATTGCTCA+TGG	0.545887	4.3:-13848466	MS.gene058844:CDS
ACAGCCAACAACACATGCCT+TGG	0.551257	4.3:+13848520	None:intergenic
GGCTAAGTAAACAGGAGCGC+CGG	0.556678	4.3:+13848624	None:intergenic
TGAGGAATGATGAAGAGCTT+GGG	0.560327	4.3:-13848498	MS.gene058844:CDS
ATCGACCTTTCTTCAAGTAA+CGG	0.579425	4.3:+13848665	None:intergenic
GGAGCTGGAGGAAGGAAAGG+CGG	0.593373	4.3:-13848757	MS.gene058844:CDS
AACGGCCAATTCTTCCGACT+GGG	0.600319	4.3:+13848683	None:intergenic
GTAACAAGATCAATCAGAGC+CGG	0.606604	4.3:-13848715	MS.gene058844:CDS
AAGAACACCACCATGAGCAA+TGG	0.622543	4.3:+13848456	None:intergenic
GCCGGACTTCAATTCCCAGT+CGG	0.639243	4.3:-13848697	MS.gene058844:CDS
CGGAGGACCAAGGAAGAAGT+CGG	0.640675	4.3:-13848737	MS.gene058844:CDS
GCGACGAAGAAAGGAGCTGG+AGG	0.642298	4.3:-13848769	MS.gene058844:CDS
GGTCGATACGCTCAACGTGT+CGG	0.644736	4.3:-13848649	MS.gene058844:CDS
GAAAATGGACGCAAGCACAA+AGG	0.663268	4.3:-13848791	None:intergenic
GGCATGTGTTGTTGGCTGTG+AGG	0.671767	4.3:-13848516	MS.gene058844:CDS
AGAAGAATCGGATTATACCA+AGG	0.672842	4.3:-13848537	MS.gene058844:CDS
TGCAAGATATTCAAGAACAG+CGG	0.685369	4.3:+13848603	None:intergenic
GGTGTTACCATTGCTCATGG+TGG	0.690637	4.3:-13848463	MS.gene058844:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAAGCACAGGGTTAATATT+GGG	+	chr4.3:13848714-13848733	None:intergenic	30.0%
	AGAAGAATCGGATTATACCA+AGG	-	chr4.3:13848607-13848626	MS.gene058844:CDS	35.0%
	ATCGACCTTTCTTCAAGTAA+CGG	+	chr4.3:13848482-13848501	None:intergenic	35.0%
	CACGTGATAACAAGAAGAAT+CGG	-	chr4.3:13848595-13848614	MS.gene058844:CDS	35.0%
	GAAAACTGAGAAAGCTGTTA+AGG	-	chr4.3:13848740-13848759	MS.gene058844:CDS	35.0%
	TGCAAGATATTCAAGAACAG+CGG	+	chr4.3:13848544-13848563	None:intergenic	35.0%
!	ACAGCTTTCTCAGTTTTCTT+AGG	+	chr4.3:13848738-13848757	None:intergenic	35.0%
!	CAAAAGCACAGGGTTAATAT+TGG	+	chr4.3:13848715-13848734	None:intergenic	35.0%
!!	TTAGCCTTAGAAGGAGATTT+AGG	+	chr4.3:13848768-13848787	None:intergenic	35.0%
!!	TTTTCTTAGGCAAAAGCACA+GGG	+	chr4.3:13848725-13848744	None:intergenic	35.0%
	ATTGGCCGTTACTTGAAGAA+AGG	-	chr4.3:13848474-13848493	MS.gene058844:CDS	40.0%
	GGCTAAGAAATCTCCTAAGA+AGG	-	chr4.3:13848782-13848801	MS.gene058844:CDS	40.0%
	GTAACAAGATCAATCAGAGC+CGG	-	chr4.3:13848429-13848448	MS.gene058844:CDS	40.0%
	TATACCAAGGCATGTGTTGT+TGG	-	chr4.3:13848620-13848639	MS.gene058844:CDS	40.0%
	TGAGGAATGATGAAGAGCTT+GGG	-	chr4.3:13848646-13848665	MS.gene058844:CDS	40.0%
	TGCAGCTGAAGTTCTTGAAT+TGG	-	chr4.3:13848560-13848579	MS.gene058844:CDS	40.0%
!!	GGAGATTTCTTAGCCTTAGA+AGG	+	chr4.3:13848777-13848796	None:intergenic	40.0%
!!	GTTTTCTTAGGCAAAAGCAC+AGG	+	chr4.3:13848726-13848745	None:intergenic	40.0%
	AAGAACACCACCATGAGCAA+TGG	+	chr4.3:13848691-13848710	None:intergenic	45.0%
	AGAACAGCGGCTAAGTAAAC+AGG	+	chr4.3:13848531-13848550	None:intergenic	45.0%
	CAATTCCCAGTCGGAAGAAT+TGG	-	chr4.3:13848456-13848475	MS.gene058844:CDS	45.0%
	GAGCTTGGGAAATTGCTTAC+TGG	-	chr4.3:13848660-13848679	MS.gene058844:CDS	45.0%
	GCTGAAGTTCTTGAATTGGC+TGG	-	chr4.3:13848564-13848583	MS.gene058844:CDS	45.0%
	GGAGCCTAAATCTCCTTCTA+AGG	-	chr4.3:13848761-13848780	MS.gene058844:CDS	45.0%
	GTGAGGAATGATGAAGAGCT+TGG	-	chr4.3:13848645-13848664	MS.gene058844:CDS	45.0%
!!	ACTGGTGTTACCATTGCTCA+TGG	-	chr4.3:13848678-13848697	MS.gene058844:CDS	45.0%
!!	CTTGTTACCGACTTCTTCCT+TGG	+	chr4.3:13848417-13848436	None:intergenic	45.0%
	AAAGGAGCTGGAGGAAGGAA+AGG	-	chr4.3:13848384-13848403	MS.gene058844:CDS	50.0%
	AACGGCCAATTCTTCCGACT+GGG	+	chr4.3:13848464-13848483	None:intergenic	50.0%
	ACAGCCAACAACACATGCCT+TGG	+	chr4.3:13848627-13848646	None:intergenic	50.0%
	AGCACAAAGGCGACGAAGAA+AGG	-	chr4.3:13848366-13848385	MS.gene058844:CDS	50.0%
	TAACGGCCAATTCTTCCGAC+TGG	+	chr4.3:13848465-13848484	None:intergenic	50.0%
	TCCGACTGGGAATTGAAGTC+CGG	+	chr4.3:13848451-13848470	None:intergenic	50.0%
!	GGTGTTACCATTGCTCATGG+TGG	-	chr4.3:13848681-13848700	MS.gene058844:CDS	50.0%
	AAGGCGACGAAGAAAGGAGC+TGG	-	chr4.3:13848372-13848391	MS.gene058844:CDS	55.0%
	CGAAGAAAGGAGCTGGAGGA+AGG	-	chr4.3:13848379-13848398	MS.gene058844:CDS	55.0%
	CGGAGGACCAAGGAAGAAGT+CGG	-	chr4.3:13848407-13848426	MS.gene058844:CDS	55.0%
	GCCGGACTTCAATTCCCAGT+CGG	-	chr4.3:13848447-13848466	MS.gene058844:CDS	55.0%
	GGTCGATACGCTCAACGTGT+CGG	-	chr4.3:13848495-13848514	MS.gene058844:CDS	55.0%
!	GGCATGTGTTGTTGGCTGTG+AGG	-	chr4.3:13848628-13848647	MS.gene058844:CDS	55.0%
!	GGCTAAGTAAACAGGAGCGC+CGG	+	chr4.3:13848523-13848542	None:intergenic	55.0%
	GAAGGAAAGGCGGAGGACCA+AGG	-	chr4.3:13848397-13848416	MS.gene058844:CDS	60.0%
	GCGACGAAGAAAGGAGCTGG+AGG	-	chr4.3:13848375-13848394	MS.gene058844:CDS	60.0%
	GGAGCTGGAGGAAGGAAAGG+CGG	-	chr4.3:13848387-13848406	MS.gene058844:CDS	60.0%
!	TACGCTCAACGTGTCGGCAC+CGG	-	chr4.3:13848501-13848520	MS.gene058844:CDS	60.0%
	GCTGGAGGAAGGAAAGGCGG+AGG	-	chr4.3:13848390-13848409	MS.gene058844:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	13848357	13848809	13848357	ID=MS.gene058844
chr4.3	mRNA	13848357	13848809	13848357	ID=MS.gene058844.t1;Parent=MS.gene058844
chr4.3	exon	13848357	13848809	13848357	ID=MS.gene058844.t1.exon1;Parent=MS.gene058844.t1
chr4.3	CDS	13848357	13848809	13848357	ID=cds.MS.gene058844.t1;Parent=MS.gene058844.t1

Gene Sequence

>MS.gene058844

ATGGACGCAAGCACAAAGGCGACGAAGAAAGGAGCTGGAGGAAGGAAAGGCGGAGGACCAAGGAAGAAGTCGGTAACAAGATCAATCAGAGCCGGACTTCAATTCCCAGTCGGAAGAATTGGCCGTTACTTGAAGAAAGGTCGATACGCTCAACGTGTCGGCACCGGCGCTCCTGTTTACTTAGCCGCTGTTCTTGAATATCTTGCAGCTGAAGTTCTTGAATTGGCTGGAAATGCAGCACGTGATAACAAGAAGAATCGGATTATACCAAGGCATGTGTTGTTGGCTGTGAGGAATGATGAAGAGCTTGGGAAATTGCTTACTGGTGTTACCATTGCTCATGGTGGTGTTCTTCCCAATATTAACCCTGTGCTTTTGCCTAAGAAAACTGAGAAAGCTGTTAAGGAGCCTAAATCTCCTTCTAAGGCTAAGAAATCTCCTAAGAAGGCTTAG

Protein sequence

>MS.gene058844.t1

MDASTKATKKGAGGRKGGGPRKKSVTRSIRAGLQFPVGRIGRYLKKGRYAQRVGTGAPVYLAAVLEYLAAEVLELAGNAARDNKKNRIIPRHVLLAVRNDEELGKLLTGVTIAHGGVLPNINPVLLPKKTEKAVKEPKSPSKAKKSPKKA